CsGy3G035320 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G035320
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationGy14Chr3: 33832498 .. 33833813 (-)
RNA-Seq ExpressionCsGy3G035320
SyntenyCsGy3G035320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAATGTATGATTCCGATTCCGATTCCAAACCAGTAAACGAAGAAGGAGATCATCCATGGAAGGAAGCCATAAACAAAGGATATGGAACGACAGGGCTTTCTAAAATGCGTCTCAGAGCACTCCCTAAAGCATTCATTCAGCCTCCAGAAAAGAGATCGAGCTCGATGATCAGAGCGTCGTCACAGGCATCAGGAGACACAATACCGATAATCGACATGTCGAAAGCAGAGGCAGCAGAATTAATCTGCGAGGCGGCAGAGAAATGGGGTTTTTTCCAGGTGATAAACCATGGAGTTCCGGCGGTACTGATGAGTGATGTAATGCATGCAGCTCGGAGATTCTTGGGTCAGGCGGCGGAGGAGAAGAGGAGGTTTCTGAAGGAGAATACGAGTTGTTCGAATGTGGTTTATATGACAAGCTTTTTCGCCGAGGCAGAGAAAGGTTTGGAGTGGAGCGATTACTTGAGTATGAATTTTGTTTCTGAGGAGGAAGCTTATGCCTTCTGGCCTGCCATTTCCAAGTAATGAACCAAGCATTACATTTCACTTTTGCTTCCCCCCCCCCCCCCCCCCTTTTTGCATATTAACAAGAATTTAAAAGTTATTTGTTCTTCAGTTTTCAAAATCTGGTTTGATTTGAAAATATTGGTAAAAAGTAGATATATAGCTGAAATTTTCATGAAACTTTTGGTTACCAATTTCCGAAAATCTAACTCAATAAATGAATGGTTTGACAATGCAGAGATGCAACAATTGAATATCTAAAGAGCTCAAACACACTAATCAAACGTGTATTAAAGATTCTAATGAACAAACTAAATGCCAACGAACTAGACGAAACAAATCAAACTCGCAGAATTCAATTCAACTACTATCCCATCAGTCCAACCCCCGATCTCAGTGTCGGAGTTCGCAGCCACTCGGACGTTTCTCTTTTGACGGTTCTGCTGCAAGACGATGTCGGCGGGCTCCACGTGCGACGAGAGATCGACAGCAAAGGAGATGAATGGGTTCAGGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGAGATTTTTTGGAGATAATGAGCAATGGAATTTACAAGAGCGTGGAGCATTGTGTGGTGTCCAATGGAAATATCGATAGAATGTCGATTCCCATATTTGTGGGTCTGCAAAATTCTCCCACTATTGGACCGTTGCCGGAGGTGCTTGCGACGGGGAAGAAAGCAGAGTACAAGGAAGTTGTGTTTTCGGATTATATTAAGTATTTCTTGAACAAGCCACTTGATGGGAAGAGCACCCTTAAGCTTGCCAAAATATAG

mRNA sequence

ATGGCTTCAATGTATGATTCCGATTCCGATTCCAAACCAGTAAACGAAGAAGGAGATCATCCATGGAAGGAAGCCATAAACAAAGGATATGGAACGACAGGGCTTTCTAAAATGCGTCTCAGAGCACTCCCTAAAGCATTCATTCAGCCTCCAGAAAAGAGATCGAGCTCGATGATCAGAGCGTCGTCACAGGCATCAGGAGACACAATACCGATAATCGACATGTCGAAAGCAGAGGCAGCAGAATTAATCTGCGAGGCGGCAGAGAAATGGGGTTTTTTCCAGGTGATAAACCATGGAGTTCCGGCGGTACTGATGAGTGATGTAATGCATGCAGCTCGGAGATTCTTGGGTCAGGCGGCGGAGGAGAAGAGGAGGTTTCTGAAGGAGAATACGAGTTGTTCGAATGTGGTTTATATGACAAGCTTTTTCGCCGAGGCAGAGAAAGGTTTGGAGTGGAGCGATTACTTGAGTATGAATTTTGTTTCTGAGGAGGAAGCTTATGCCTTCTGGCCTGCCATTTCCAAAGATGCAACAATTGAATATCTAAAGAGCTCAAACACACTAATCAAACGTGTATTAAAGATTCTAATGAACAAACTAAATGCCAACGAACTAGACGAAACAAATCAAACTCGCAGAATTCAATTCAACTACTATCCCATCAGTCCAACCCCCGATCTCAGTGTCGGAGTTCGCAGCCACTCGGACGTTTCTCTTTTGACGGTTCTGCTGCAAGACGATGTCGGCGGGCTCCACGTGCGACGAGAGATCGACAGCAAAGGAGATGAATGGGTTCAGGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGAGATTTTTTGGAGATAATGAGCAATGGAATTTACAAGAGCGTGGAGCATTGTGTGGTGTCCAATGGAAATATCGATAGAATGTCGATTCCCATATTTGTGGGTCTGCAAAATTCTCCCACTATTGGACCGTTGCCGGAGGTGCTTGCGACGGGGAAGAAAGCAGAGTACAAGGAAGTTGTGTTTTCGGATTATATTAAGTATTTCTTGAACAAGCCACTTGATGGGAAGAGCACCCTTAAGCTTGCCAAAATATAG

Coding sequence (CDS)

ATGGCTTCAATGTATGATTCCGATTCCGATTCCAAACCAGTAAACGAAGAAGGAGATCATCCATGGAAGGAAGCCATAAACAAAGGATATGGAACGACAGGGCTTTCTAAAATGCGTCTCAGAGCACTCCCTAAAGCATTCATTCAGCCTCCAGAAAAGAGATCGAGCTCGATGATCAGAGCGTCGTCACAGGCATCAGGAGACACAATACCGATAATCGACATGTCGAAAGCAGAGGCAGCAGAATTAATCTGCGAGGCGGCAGAGAAATGGGGTTTTTTCCAGGTGATAAACCATGGAGTTCCGGCGGTACTGATGAGTGATGTAATGCATGCAGCTCGGAGATTCTTGGGTCAGGCGGCGGAGGAGAAGAGGAGGTTTCTGAAGGAGAATACGAGTTGTTCGAATGTGGTTTATATGACAAGCTTTTTCGCCGAGGCAGAGAAAGGTTTGGAGTGGAGCGATTACTTGAGTATGAATTTTGTTTCTGAGGAGGAAGCTTATGCCTTCTGGCCTGCCATTTCCAAAGATGCAACAATTGAATATCTAAAGAGCTCAAACACACTAATCAAACGTGTATTAAAGATTCTAATGAACAAACTAAATGCCAACGAACTAGACGAAACAAATCAAACTCGCAGAATTCAATTCAACTACTATCCCATCAGTCCAACCCCCGATCTCAGTGTCGGAGTTCGCAGCCACTCGGACGTTTCTCTTTTGACGGTTCTGCTGCAAGACGATGTCGGCGGGCTCCACGTGCGACGAGAGATCGACAGCAAAGGAGATGAATGGGTTCAGGTGAAGCCAGTAGCAGAGTCTTTAGTAATCAACATTGGAGATTTTTTGGAGATAATGAGCAATGGAATTTACAAGAGCGTGGAGCATTGTGTGGTGTCCAATGGAAATATCGATAGAATGTCGATTCCCATATTTGTGGGTCTGCAAAATTCTCCCACTATTGGACCGTTGCCGGAGGTGCTTGCGACGGGGAAGAAAGCAGAGTACAAGGAAGTTGTGTTTTCGGATTATATTAAGTATTTCTTGAACAAGCCACTTGATGGGAAGAGCACCCTTAAGCTTGCCAAAATATAG

Protein sequence

MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI*
Homology
BLAST of CsGy3G035320 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 7.3e-94
Identity = 177/347 (51.01%), Postives = 233/347 (67.15%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSKAE---AAE 85
           + KG G  GLS+  ++ALP+ +IQP E+R   +I      + + IP+IDMS  +    AE
Sbjct: 21  VYKGNGVKGLSETGIKALPEQYIQPLEER---LINKFVNETDEAIPVIDMSNPDEDRVAE 80

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            +C+AAEKWGFFQVINHGVP  ++ DV  A  +F     EEKR+F KEN+  + V + TS
Sbjct: 81  AVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTS 140

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F   AE+ LEW DYLS+ FVSE EA  FWP I ++ T+EY+  S  +++R+L+ L   LN
Sbjct: 141 FSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLN 200

Query: 206 ANELDETNQTR-----RIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             ELDET ++      R+  NYYPI P PDL+VGV  HSDVS LT+LLQD +GGLHVR  
Sbjct: 201 VKELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVR-- 260

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                  WV V PVA S VINIGD ++IMSNG+YKSVEH V++NG  +R+S+PIFV  + 
Sbjct: 261 -SLASGNWVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHRVLANGYNNRISVPIFVNPKP 320

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
              IGPLPEV+A G++  Y++V++SDY+KYF  K  DGK T+  AKI
Sbjct: 321 ESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKKTVDYAKI 361

BLAST of CsGy3G035320 vs. ExPASy Swiss-Prot
Match: G9M9M1 (Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-92
Identity = 171/348 (49.14%), Postives = 242/348 (69.54%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK----AEAA 85
           +++G G  GL+ M L ALPK ++QP E+R    I  S+    DTIP+ID+S+     +  
Sbjct: 17  VHEGNGVKGLADMGLEALPKQYVQPEEER----ITTSTVIVDDTIPVIDLSEWGSDPKVG 76

Query: 86  ELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMT 145
           ++ICEAAEKWGFFQ++NHGVP  ++ +V  A  RF    AEEK +  K+N+  +NV Y T
Sbjct: 77  DMICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHCKDNSPSNNVRYGT 136

Query: 146 SFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKL 205
           SF   AEK LEW D+LS+ +VS+EEA A WP+  +D  + ++++ + +IKR+LK LM  L
Sbjct: 137 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 196

Query: 206 NANELDETNQ-----TRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRR 265
           N  E+D T +     ++RI  NYYP  P P+L+VGV  HSDVS LT+LLQD +GGL+VR+
Sbjct: 197 NVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVRK 256

Query: 266 EIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQ 325
            +DS  D WV V P+  ++VIN+GD L+I+SNG YKS+EH V++NG+ +R+S+PIFV  +
Sbjct: 257 -LDS--DTWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNPR 316

Query: 326 NSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
            +  IGPLPE+L +G+KA YK V++SDY+K+F  K  DGK T+  AKI
Sbjct: 317 PNDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

BLAST of CsGy3G035320 vs. ExPASy Swiss-Prot
Match: G9M9M2 (Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-92
Identity = 172/348 (49.43%), Postives = 243/348 (69.83%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK----AEAA 85
           +++G G  GL+ M L++LPK +IQP E+R    I  S+    DTIP+ID+S+     +  
Sbjct: 17  VHEGNGVKGLADMGLQSLPKQYIQPAEER----ITTSTVIVDDTIPVIDLSEWGSDPKVG 76

Query: 86  ELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMT 145
           +LICEAAEKWGFFQ++NHGVP  ++ +V  A  RF    AEEK +  K+N+  +NV Y T
Sbjct: 77  DLICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSPSNNVRYGT 136

Query: 146 SFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKL 205
           SF   AEK LEW D+LS+ +VS+EEA A WP+  +D  + ++++ + +IKR+LK LM  L
Sbjct: 137 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 196

Query: 206 NANELDETNQ-----TRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRR 265
           N  E+D T +     ++RI  NYYP  P P+L+VGV  HSDVS LT+LLQD +GGL+VR+
Sbjct: 197 NVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVRK 256

Query: 266 EIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQ 325
            +DS  D WV V P+  ++VIN+GD L+I+SNG YKS+EH V++NG+ +R+S+PIFV  +
Sbjct: 257 -LDS--DTWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNPR 316

Query: 326 NSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
            +  IGPLPE+L +G+KA YK V++SDY+K+F  K  DGK T+  AKI
Sbjct: 317 PNDIIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

BLAST of CsGy3G035320 vs. ExPASy Swiss-Prot
Match: G9M9M0 (Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.3e-92
Identity = 171/348 (49.14%), Postives = 242/348 (69.54%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK----AEAA 85
           +++G G  GL+ M L ALPK ++QP E+R    I  S+    DTIP+ID+S+     +  
Sbjct: 17  VHEGNGVKGLADMGLEALPKQYVQPEEER----ITTSTVIVDDTIPVIDLSEWGSDPKVG 76

Query: 86  ELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMT 145
           ++ CEAAEKWGFFQ++NHGVP  ++ +V  A  RF    AEEK +  K+N+  +NV Y T
Sbjct: 77  DMNCEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSPSNNVRYGT 136

Query: 146 SFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKL 205
           SF   AEK LEW D+LS+ +VS+EEA A WP+  +D  + ++++ + +IKR+LK LM  L
Sbjct: 137 SFTPHAEKALEWKDFLSLFYVSDEEAAALWPSACRDEALTFMRNCDAVIKRLLKSLMKGL 196

Query: 206 NANELDETNQ-----TRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRR 265
           N  E+D T +     ++RI  NYYP  P P+L+VGV  HSDVS LT+LLQD +GGL+VR+
Sbjct: 197 NVTEIDGTKESLLMGSKRINMNYYPKCPNPELTVGVGRHSDVSTLTILLQDQIGGLYVRK 256

Query: 266 EIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQ 325
            +DS  DEWV V P+  ++VIN+GD L+I+SNG YKS+EH V++NG+ +R+S+PIFV  +
Sbjct: 257 -LDS--DEWVHVPPINGAIVINVGDALQILSNGRYKSIEHRVIANGSNNRISVPIFVNPR 316

Query: 326 NSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
            +  IGPLPE+L +G+KA YK V++SDY+K+F  K  DGK T+  AKI
Sbjct: 317 PNDVIGPLPELLESGEKAVYKNVLYSDYVKHFFRKAHDGKETVDFAKI 357

BLAST of CsGy3G035320 vs. ExPASy Swiss-Prot
Match: G9M9M4 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-1 OS=Ipomoea batatas OX=4120 GN=F6H2-1-1 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 7.5e-91
Identity = 169/347 (48.70%), Postives = 236/347 (68.01%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSKAE---AAE 85
           + +G+G  GLS++ L+ LP  ++ PPE+R SSM       S D+IP+ID+S  E    A+
Sbjct: 18  VKQGHGVKGLSELGLQTLPNQYVHPPEERLSSM----DVVSDDSIPVIDVSNWEDPKVAK 77

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
           LIC+AAEK GFFQ++NHG+P  ++     A  RF  + AEEK+++ KEN   S+V Y TS
Sbjct: 78  LICDAAEKRGFFQIVNHGIPLEMLEKAKAATYRFFREPAEEKKKYSKENCPTSHVRYSTS 137

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  + EK LEW D+LSM +VS+EEA  +WP   +D  +EYLKS   + +++L+ LM  LN
Sbjct: 138 FLPQIEKALEWKDHLSMFYVSDEEAAQYWPPSCRDDALEYLKSCEMVSRKLLEALMQGLN 197

Query: 206 ANELDETNQ-----TRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
            NE+D+  +     +RRI  NYYP  P PDL+VGV  HSD+S LT+LLQDD+GGL+VR+ 
Sbjct: 198 VNEIDDAKESLLMGSRRININYYPKCPNPDLTVGVGRHSDISTLTLLLQDDIGGLYVRK- 257

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
              + + W  V PV  +LVINIGD L+IMSNG YKS+EH V++N   DR+S+P+FV  + 
Sbjct: 258 --LEHEAWSHVPPVKGALVINIGDALQIMSNGRYKSIEHRVLANETNDRISVPVFVNPKP 317

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
           +  +GPLPEVLA+G+K  YK V++SDY K+F  K  +GK T+  A+I
Sbjct: 318 NDIVGPLPEVLASGEKPVYKPVLYSDYAKHFYRKAHNGKDTIAFARI 357

BLAST of CsGy3G035320 vs. NCBI nr
Match: XP_011652189.1 (feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical protein Csa_001767 [Cucumis sativus])

HSP 1 Score: 726 bits (1875), Expect = 3.30e-264
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60
           MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR
Sbjct: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60

Query: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120
           ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA
Sbjct: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120

Query: 121 AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATI 180
           AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATI
Sbjct: 121 AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATI 180

Query: 181 EYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSL 240
           EYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSL
Sbjct: 181 EYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSL 240

Query: 241 LTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVS 300
           LTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVS
Sbjct: 241 LTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVS 300

Query: 301 NGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLK 360
           NGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLK
Sbjct: 301 NGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLK 360

Query: 361 LAKI 364
           LAKI
Sbjct: 361 LAKI 364

BLAST of CsGy3G035320 vs. NCBI nr
Match: KAF9865102.1 (hypothetical protein H0E87_024538 [Populus deltoides])

HSP 1 Score: 370 bits (950), Expect = 1.42e-123
Identity = 187/347 (53.89%), Postives = 246/347 (70.89%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK---AEAAE 85
           +NKG G  GLS+M L +LPK +IQP E+R       +   S ++IPIIDMSK    + AE
Sbjct: 20  LNKGNGVKGLSEMGLESLPKQYIQPLEERMCG----TKIMSHESIPIIDMSKWDDPKVAE 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            ICEAAEKWGFFQ+INHGVP  ++ +V  A  +F    AEEKR++LKE +  +NV + TS
Sbjct: 80  AICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSPSNNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  EAEK LEW DYLS+ +VSE+EA A WPA+ KD  +EY+K S T+I+++L +LM  LN
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASALWPAVCKDQVLEYMKRSETVIRKLLDVLMKNLN 199

Query: 206 ANELDETNQT-----RRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             E+DET ++     +R   NYYPI P P+L+VGV  HSDVS LT LLQDD+GGL+VR  
Sbjct: 200 VTEIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVR-- 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                D W+ V PV+ S+VIN+GD L+IMSNG YKS+EH V++NG+ +R+S+PIF+  + 
Sbjct: 260 --GNNDSWIHVPPVSGSIVINVGDALQIMSNGRYKSIEHRVIANGSNNRISVPIFINPRP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 364
           S  I P PEVLA+G+KA YKEV++SDY+K+F  K  DGK T+ LAKI
Sbjct: 320 SDKISPFPEVLASGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

BLAST of CsGy3G035320 vs. NCBI nr
Match: KAE9462342.1 (hypothetical protein C3L33_05761, partial [Rhododendron williamsianum])

HSP 1 Score: 370 bits (950), Expect = 1.68e-123
Identity = 194/372 (52.15%), Postives = 258/372 (69.35%), Query Frame = 0

Query: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60
           ++ + DSDSDS  V        K A+ +G G  GLS++ L+ LPK +IQP E+R SSM  
Sbjct: 5   LSLISDSDSDSLDVT-------KFAVYQGNGVKGLSELGLKTLPKQYIQPVEERMSSM-- 64

Query: 61  ASSQASGDTIPIIDMSK---AEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFL 120
             S    ++IPIIDM+     + A+ IC+AAEKWGFFQ+INHGVP  ++ DV  A  RF 
Sbjct: 65  --SHIEXESIPIIDMANWDDPKVADAICDAAEKWGFFQIINHGVPIEVLEDVKTATHRFF 124

Query: 121 GQAAEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKD 180
           G  AEEK ++ KEN+  +NV Y TSF  +AEK LEW DYLS+ +VS+ EA + WP+  KD
Sbjct: 125 GLPAEEKGKYSKENSCTNNVRYGTSFSPQAEKALEWKDYLSLFYVSDGEAASRWPSQCKD 184

Query: 181 ATIEYLKSSNTLIKRVLKILMNKLNANELDETNQT-----RRIQFNYYPISPTPDLSVGV 240
             +E+++ S+  IKR+L+ L  +LN  E+DET+QT     +RI  NYYPI P P+L+VGV
Sbjct: 185 QALEFMRRSDCAIKRLLEALTKRLNVKEMDETHQTLLMGSKRINLNYYPICPNPELTVGV 244

Query: 241 RSHSDVSLLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYK 300
             HSDVS LT+LLQD++GGL+VR+  D   D W+ V P++ SLVINIGD L+IMSNG YK
Sbjct: 245 GRHSDVSTLTILLQDEIGGLYVRKLDDE--DTWIHVPPISGSLVINIGDALQIMSNGRYK 304

Query: 301 SVEHCVVSNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKP 360
           SVEH V++NG+ +R+S+PIFV    S  IGPLPEVL  G+K  YK+V++SDY+KYF  K 
Sbjct: 305 SVEHRVMANGSKNRISVPIFVNPXPSAIIGPLPEVLEGGEKPVYKQVLYSDYVKYFFRKG 363

Query: 361 LDGKSTLKLAKI 364
            DGK T++ AKI
Sbjct: 365 HDGKDTVEFAKI 363

BLAST of CsGy3G035320 vs. NCBI nr
Match: XP_034923410.1 (feruloyl CoA ortho-hydroxylase F6H1-3-like [Populus alba] >KAG6792990.1 hypothetical protein POTOM_002157 [Populus tomentosa] >TKS07853.1 oxidoreductase family protein [Populus alba])

HSP 1 Score: 369 bits (948), Expect = 2.86e-123
Identity = 187/347 (53.89%), Postives = 244/347 (70.32%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK---AEAAE 85
           +NKG G  GLS M L +LPK +IQP E+R       +   S ++IPIIDMSK    + AE
Sbjct: 20  LNKGNGVKGLSDMGLESLPKQYIQPLEERMCG----TKIMSHESIPIIDMSKWDDPKVAE 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            ICEAAEKWGFFQ+INHGVP  ++ +V  A  +F    AEEKR++LKE +  +NV + TS
Sbjct: 80  AICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSPSNNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  EAEK LEW DYLS+ +VSE+EA A WPA+ KD  +EY+K S  +I+++L +LM  LN
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASAMWPAVCKDQVLEYMKRSEIVIRKLLDVLMKNLN 199

Query: 206 ANELDETNQT-----RRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             E+DET ++     +R   NYYPI P P+L+VGV  HSDVS LT LLQDD+GGL+VR  
Sbjct: 200 VTEIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVR-- 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                D W+ V PV+ S+VIN+GD L+IMSNG YKS+EH V++NGN +R+S+PIF+  + 
Sbjct: 260 --GNNDSWIHVPPVSGSIVINVGDALQIMSNGRYKSIEHRVIANGNSNRISVPIFINPRP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 364
           S  I P PEVLA+G+KA YKEV++SDY+K+F  K  DGK T+ LAKI
Sbjct: 320 SDKISPFPEVLASGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

BLAST of CsGy3G035320 vs. NCBI nr
Match: XP_024449267.1 (feruloyl CoA ortho-hydroxylase 1-like [Populus trichocarpa] >PNS22122.1 hypothetical protein POPTR_T170500 [Populus trichocarpa])

HSP 1 Score: 368 bits (944), Expect = 1.15e-122
Identity = 187/347 (53.89%), Postives = 244/347 (70.32%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK---AEAAE 85
           +NKG G  GLS+M L +LPK +IQP E+R       +   S ++IPIIDMSK    + AE
Sbjct: 20  LNKGNGVKGLSEMGLESLPKQYIQPLEERMCG----TKIMSHESIPIIDMSKWDDPKVAE 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            ICEAAEKWGFFQ+INHGVP  ++ +V  A  +F    AEEKR++LKE +  +NV + TS
Sbjct: 80  AICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSPSNNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  EAEK LEW DYLS+ +VSE+EA A WPA+ KD  +EY+K S T+I+++L +LM  LN
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASALWPAVCKDQVLEYMKRSETVIRKLLDVLMKNLN 199

Query: 206 ANELDETNQT-----RRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             E+DET ++     +R   NYYPI P P+L+VGV  HSDVS LT LLQDD+GGL+VR  
Sbjct: 200 VTEIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVR-- 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                D W+ V PV+ S+VIN+GD L+IMSNG YKS+EH V++NG+ +R+S+PIF+    
Sbjct: 260 --GNNDSWIHVPPVSCSIVINVGDALQIMSNGRYKSIEHRVIANGSNNRISVPIFINPMP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 364
           S  I P PEVLA G+KA YKEV++SDY+K+F  K  DGK T+ LAKI
Sbjct: 320 SDKISPFPEVLAGGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

BLAST of CsGy3G035320 vs. ExPASy TrEMBL
Match: A0A0A0LFH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G821020 PE=3 SV=1)

HSP 1 Score: 726 bits (1875), Expect = 1.60e-264
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60
           MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR
Sbjct: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60

Query: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120
           ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA
Sbjct: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120

Query: 121 AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATI 180
           AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATI
Sbjct: 121 AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATI 180

Query: 181 EYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSL 240
           EYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSL
Sbjct: 181 EYLKSSNTLIKRVLKILMNKLNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSL 240

Query: 241 LTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVS 300
           LTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVS
Sbjct: 241 LTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVS 300

Query: 301 NGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLK 360
           NGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLK
Sbjct: 301 NGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLK 360

Query: 361 LAKI 364
           LAKI
Sbjct: 361 LAKI 364

BLAST of CsGy3G035320 vs. ExPASy TrEMBL
Match: A0A6A4LYV6 (Fe2OG dioxygenase domain-containing protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_05761 PE=3 SV=1)

HSP 1 Score: 370 bits (950), Expect = 8.15e-124
Identity = 194/372 (52.15%), Postives = 258/372 (69.35%), Query Frame = 0

Query: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60
           ++ + DSDSDS  V        K A+ +G G  GLS++ L+ LPK +IQP E+R SSM  
Sbjct: 5   LSLISDSDSDSLDVT-------KFAVYQGNGVKGLSELGLKTLPKQYIQPVEERMSSM-- 64

Query: 61  ASSQASGDTIPIIDMSK---AEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFL 120
             S    ++IPIIDM+     + A+ IC+AAEKWGFFQ+INHGVP  ++ DV  A  RF 
Sbjct: 65  --SHIEXESIPIIDMANWDDPKVADAICDAAEKWGFFQIINHGVPIEVLEDVKTATHRFF 124

Query: 121 GQAAEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKD 180
           G  AEEK ++ KEN+  +NV Y TSF  +AEK LEW DYLS+ +VS+ EA + WP+  KD
Sbjct: 125 GLPAEEKGKYSKENSCTNNVRYGTSFSPQAEKALEWKDYLSLFYVSDGEAASRWPSQCKD 184

Query: 181 ATIEYLKSSNTLIKRVLKILMNKLNANELDETNQT-----RRIQFNYYPISPTPDLSVGV 240
             +E+++ S+  IKR+L+ L  +LN  E+DET+QT     +RI  NYYPI P P+L+VGV
Sbjct: 185 QALEFMRRSDCAIKRLLEALTKRLNVKEMDETHQTLLMGSKRINLNYYPICPNPELTVGV 244

Query: 241 RSHSDVSLLTVLLQDDVGGLHVRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYK 300
             HSDVS LT+LLQD++GGL+VR+  D   D W+ V P++ SLVINIGD L+IMSNG YK
Sbjct: 245 GRHSDVSTLTILLQDEIGGLYVRKLDDE--DTWIHVPPISGSLVINIGDALQIMSNGRYK 304

Query: 301 SVEHCVVSNGNIDRMSIPIFVGLQNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKP 360
           SVEH V++NG+ +R+S+PIFV    S  IGPLPEVL  G+K  YK+V++SDY+KYF  K 
Sbjct: 305 SVEHRVMANGSKNRISVPIFVNPXPSAIIGPLPEVLEGGEKPVYKQVLYSDYVKYFFRKG 363

Query: 361 LDGKSTLKLAKI 364
            DGK T++ AKI
Sbjct: 365 HDGKDTVEFAKI 363

BLAST of CsGy3G035320 vs. ExPASy TrEMBL
Match: A0A4U5QC14 (Oxidoreductase family protein OS=Populus alba OX=43335 GN=D5086_0000108930 PE=3 SV=1)

HSP 1 Score: 369 bits (948), Expect = 1.38e-123
Identity = 187/347 (53.89%), Postives = 244/347 (70.32%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK---AEAAE 85
           +NKG G  GLS M L +LPK +IQP E+R       +   S ++IPIIDMSK    + AE
Sbjct: 20  LNKGNGVKGLSDMGLESLPKQYIQPLEERMCG----TKIMSHESIPIIDMSKWDDPKVAE 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            ICEAAEKWGFFQ+INHGVP  ++ +V  A  +F    AEEKR++LKE +  +NV + TS
Sbjct: 80  AICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSPSNNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  EAEK LEW DYLS+ +VSE+EA A WPA+ KD  +EY+K S  +I+++L +LM  LN
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASAMWPAVCKDQVLEYMKRSEIVIRKLLDVLMKNLN 199

Query: 206 ANELDETNQT-----RRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             E+DET ++     +R   NYYPI P P+L+VGV  HSDVS LT LLQDD+GGL+VR  
Sbjct: 200 VTEIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVR-- 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                D W+ V PV+ S+VIN+GD L+IMSNG YKS+EH V++NGN +R+S+PIF+  + 
Sbjct: 260 --GNNDSWIHVPPVSGSIVINVGDALQIMSNGRYKSIEHRVIANGNSNRISVPIFINPRP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 364
           S  I P PEVLA+G+KA YKEV++SDY+K+F  K  DGK T+ LAKI
Sbjct: 320 SDKISPFPEVLASGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

BLAST of CsGy3G035320 vs. ExPASy TrEMBL
Match: A0A2K1R4B1 (Fe2OG dioxygenase domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_T170500 PE=3 SV=1)

HSP 1 Score: 368 bits (944), Expect = 5.58e-123
Identity = 187/347 (53.89%), Postives = 244/347 (70.32%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSK---AEAAE 85
           +NKG G  GLS+M L +LPK +IQP E+R       +   S ++IPIIDMSK    + AE
Sbjct: 20  LNKGNGVKGLSEMGLESLPKQYIQPLEERMCG----TKIMSHESIPIIDMSKWDDPKVAE 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            ICEAAEKWGFFQ+INHGVP  ++ +V  A  +F    AEEKR++LKE +  +NV + TS
Sbjct: 80  AICEAAEKWGFFQIINHGVPIEVLENVKEATHQFFRLPAEEKRKYLKEFSPSNNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  EAEK LEW DYLS+ +VSE+EA A WPA+ KD  +EY+K S T+I+++L +LM  LN
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEDEASALWPAVCKDQVLEYMKRSETVIRKLLDVLMKNLN 199

Query: 206 ANELDETNQT-----RRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             E+DET ++     +R   NYYPI P P+L+VGV  HSDVS LT LLQDD+GGL+VR  
Sbjct: 200 VTEIDETKESLLMGSKRTNLNYYPICPNPELTVGVGRHSDVSTLTFLLQDDIGGLYVR-- 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                D W+ V PV+ S+VIN+GD L+IMSNG YKS+EH V++NG+ +R+S+PIF+    
Sbjct: 260 --GNNDSWIHVPPVSCSIVINVGDALQIMSNGRYKSIEHRVIANGSNNRISVPIFINPMP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 364
           S  I P PEVLA G+KA YKEV++SDY+K+F  K  DGK T+ LAKI
Sbjct: 320 SDKISPFPEVLAGGEKAVYKEVLYSDYVKHFFRKAHDGKKTIDLAKI 358

BLAST of CsGy3G035320 vs. ExPASy TrEMBL
Match: A0A2N9GN50 (Fe2OG dioxygenase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS28825 PE=3 SV=1)

HSP 1 Score: 366 bits (940), Expect = 2.25e-122
Identity = 187/347 (53.89%), Postives = 244/347 (70.32%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMS---KAEAAE 85
           ++KG G  GLS+M L+ LP  +IQP E+R S     S+    ++IPIIDMS   + + AE
Sbjct: 20  VSKGNGVKGLSEMGLKTLPNQYIQPLEERLSM----SNIMPLESIPIIDMSNWDEPKVAE 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            IC+AAEKWGFFQ+INHGVP  ++ +V  A   F G  AEEKR+FLKEN+  +NV + TS
Sbjct: 80  SICDAAEKWGFFQIINHGVPIEVLENVKDATHSFFGLPAEEKRKFLKENSPTNNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F  EAEK LEW DYLS+ +VSE EA   WP + KD  +EY+K S  +IKR+L+ LM +LN
Sbjct: 140 FSPEAEKALEWKDYLSLFYVSEHEASTLWPLVCKDQVLEYMKRSELVIKRLLETLMKRLN 199

Query: 206 ANELDETNQT-----RRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             E+DE  ++     +RI  NYYPI P P+L+VGV  HSDVS LT+LLQDD+GGL+VR  
Sbjct: 200 VKEIDEIKESLLMGSKRINLNYYPICPNPELTVGVGRHSDVSTLTILLQDDIGGLYVR-- 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                D W+ V P++ SLVINIGD L+IMSNG YKS+EH VV+NG+ +R+S+PIFV  + 
Sbjct: 260 --GNNDSWIHVSPISGSLVINIGDALQIMSNGRYKSIEHRVVANGSKNRISVPIFVNPRP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 364
              IGP  EVLA G+KA YK+V++SDY+K+F NK  DGK T++ AKI
Sbjct: 320 GDMIGPFQEVLAGGEKAAYKQVLYSDYVKHFFNKAHDGKQTVEFAKI 358

BLAST of CsGy3G035320 vs. TAIR 10
Match: AT3G13610.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 5.2e-95
Identity = 177/347 (51.01%), Postives = 233/347 (67.15%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSKAE---AAE 85
           + KG G  GLS+  ++ALP+ +IQP E+R   +I      + + IP+IDMS  +    AE
Sbjct: 21  VYKGNGVKGLSETGIKALPEQYIQPLEER---LINKFVNETDEAIPVIDMSNPDEDRVAE 80

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            +C+AAEKWGFFQVINHGVP  ++ DV  A  +F     EEKR+F KEN+  + V + TS
Sbjct: 81  AVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTS 140

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F   AE+ LEW DYLS+ FVSE EA  FWP I ++ T+EY+  S  +++R+L+ L   LN
Sbjct: 141 FSPLAEQALEWKDYLSLFFVSEAEAEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLN 200

Query: 206 ANELDETNQTR-----RIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             ELDET ++      R+  NYYPI P PDL+VGV  HSDVS LT+LLQD +GGLHVR  
Sbjct: 201 VKELDETKESLFMGSIRVNLNYYPICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVR-- 260

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
                  WV V PVA S VINIGD ++IMSNG+YKSVEH V++NG  +R+S+PIFV  + 
Sbjct: 261 -SLASGNWVHVPPVAGSFVINIGDAMQIMSNGLYKSVEHRVLANGYNNRISVPIFVNPKP 320

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
              IGPLPEV+A G++  Y++V++SDY+KYF  K  DGK T+  AKI
Sbjct: 321 ESVIGPLPEVIANGEEPIYRDVLYSDYVKYFFRKAHDGKKTVDYAKI 361

BLAST of CsGy3G035320 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 1.8e-87
Identity = 162/347 (46.69%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMSKAE---AAE 85
           +++G G  GLS+  ++ LP  +IQP E+R  +      + S ++IP+ID+S  +    ++
Sbjct: 20  VHEGNGVKGLSETGIKVLPDQYIQPFEERLINF--HVKEDSDESIPVIDISNLDEKSVSK 79

Query: 86  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 145
            +C+AAE+WGFFQVINHGV   ++ ++  A  RF G   EEKR+F +E +  +NV + TS
Sbjct: 80  AVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRKFSREKSLSTNVRFGTS 139

Query: 146 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNKLN 205
           F   AEK LEW DYLS+ FVSE EA   WP   +  T+EY+  +  L+K++L+ L   LN
Sbjct: 140 FSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRSETLEYMNETKPLVKKLLRFLGENLN 199

Query: 206 ANELDETNQ-----TRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRRE 265
             ELD+T +     + RI  NYYPI P P+L+VGV  HSDVS LT+LLQD++GGLHVR  
Sbjct: 200 VKELDKTKESFFMGSTRINLNYYPICPNPELTVGVGRHSDVSSLTILLQDEIGGLHVRSL 259

Query: 266 IDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQN 325
              +   WV V P++ SLVINIGD ++IMSNG YKSVEH V++NG+ +R+S+PIFV  + 
Sbjct: 260 TTGR---WVHVPPISGSLVINIGDAMQIMSNGRYKSVEHRVLANGSYNRISVPIFVSPKP 319

Query: 326 SPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
              IGPL EV+  G+K  YK+++++DY+K+F  K  DGK T+  A I
Sbjct: 320 ESVIGPLLEVIENGEKPVYKDILYTDYVKHFFRKAHDGKKTIDFANI 361

BLAST of CsGy3G035320 vs. TAIR 10
Match: AT3G12900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.9e-68
Identity = 138/344 (40.12%), Postives = 200/344 (58.14%), Query Frame = 0

Query: 26  INKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASG--DTIPIIDMSKAEAAEL 85
           + +G G  G+    L ++P+ F+QP  +R  +    + +A+   D   +      E A+ 
Sbjct: 16  VREGNGVKGMIDSGLSSVPRPFVQPLSERIPTQKALTCEATQPIDLSNLDGPQHKEVAKQ 75

Query: 86  ICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTSF 145
           I EAAE  GFFQV+NHGV   L+  +  +A  F  QA EEK  +LKE +    V Y TSF
Sbjct: 76  IVEAAETLGFFQVVNHGVSVELLELLKSSAHEFFAQAPEEKSMYLKEVSPSKLVKYGTSF 135

Query: 146 FAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNK--- 205
             + EK +EW DY+SM + ++ EA   WP   ++  +E+L SS  ++K V+ ILM     
Sbjct: 136 VPDKEKAIEWKDYVSMLYTNDSEALQHWPQPCREVALEFLNSSMEMVKNVVNILMENVGV 195

Query: 206 -LNANELDETNQTRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRREID 265
            L   +++    T+ +  NYYP  P+P+L+VGV  HSD+ +LTVLLQD +GGL+V+ +  
Sbjct: 196 TLEEEKMNGLMGTKMVNMNYYPTCPSPELTVGVGRHSDMGMLTVLLQDGIGGLYVKLD-- 255

Query: 266 SKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVGLQNSP 325
               EW ++ PV  +LVINIGD L+I+SNG YKS EH V +     R+S+PIF     S 
Sbjct: 256 --NGEWAEIPPVHGALVINIGDTLQILSNGKYKSAEHRVRTTNIGSRVSVPIFTAPNPSQ 315

Query: 326 TIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAK 364
            +GPLPEV+     A YKE +F DY+  F  +P DGK +L  A+
Sbjct: 316 KVGPLPEVVKRDGVARYKEFLFQDYMNNFFGQPHDGKKSLDFAR 355

BLAST of CsGy3G035320 vs. TAIR 10
Match: AT5G12270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-66
Identity = 138/352 (39.20%), Postives = 207/352 (58.81%), Query Frame = 0

Query: 26  INKGYGTTGL-SKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDMS---KAEAA 85
           +N+  G  GL   + L  LP  +IQPP++R +S         G  +P+ID+S   +   A
Sbjct: 15  VNQKNGVKGLVDFLTLTTLPSPYIQPPQERFTS----DKILLGSPVPVIDVSNWNEPHVA 74

Query: 86  ELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMT 145
             IC AA K G FQ++NHG+       V+ AAR F    AEE+RR+ + ++        T
Sbjct: 75  REICHAASKLGLFQIVNHGIAPAEFKGVIAAARGFFELPAEERRRYWRGSSVSETAWLTT 134

Query: 146 SFFAEAEKGLEWSDYLSMNFVSEEEAY-AFWPAISKDATIEYLKSSNTLIKRVLKILMNK 205
           SF    E  LEW D+L   ++ +   + A WP++ K+  I++ K    + +R+L IL+N 
Sbjct: 135 SFNPCIESVLEWRDFLKFEYLPQRHDFAATWPSVCKEQVIDHFKRIKPITERILNILINN 194

Query: 206 LNANELDETNQ-----TRRIQFNYYPISPTPDLSVGVRSHSDVSLLTVLLQDD--VGGLH 265
           LN   +DE+N+     T R+ FNYYP  P P L++G   HSD++ LT+LLQ+D  +  L+
Sbjct: 195 LN-TIIDESNKETLMGTMRMNFNYYPKCPEPSLAIGTGRHSDINTLTLLLQEDGVLSSLY 254

Query: 266 VRREIDSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFV 325
            R   D  GD+W+ V P+  ++V+NIGD L+I+SN  Y+SVEHCVV N    R+SIP+F 
Sbjct: 255 ARATED--GDKWIHVPPIPGAIVVNIGDVLQILSNDRYRSVEHCVVVNKYCSRVSIPVFC 314

Query: 326 GLQNSPTIGPLPEVL-ATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
           G  +   I PLPEVL    + A Y+++V+SDY+K+F  +P DGK T++  K+
Sbjct: 315 GPVHDSVIEPLPEVLDKNNEMARYRKIVYSDYLKFFFGRPHDGKKTIESIKL 359

BLAST of CsGy3G035320 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.9e-45
Identity = 117/349 (33.52%), Postives = 175/349 (50.14%), Query Frame = 0

Query: 31  GTTGLSKMRLRALPKAFIQPPEKRSSSMIRASSQASGDTIPIIDM--------SKAEAAE 90
           G  GL    +  +P  F  PP   +S    +SS  S   IP ID+        ++    E
Sbjct: 26  GVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFS---IPTIDLKGGGTDSITRRSLVE 85

Query: 91  LICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQAAEEKRRFLKENTSCSNVVYMTS 150
            I +AAEKWGFFQVINHG+P  ++  ++   R F  Q  E K+ F   + + S +VY ++
Sbjct: 86  KIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDPA-SKMVYSSN 145

Query: 151 FFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISKDATIEYLKSSNTLIKRVLKILMNK-- 210
           F   +     W D L      +       PA   +  IEY K    L K + ++L     
Sbjct: 146 FDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLLFELLSEALG 205

Query: 211 LNANELDETNQTRRIQF--NYYPISPTPDLSVGVRSHSDVSLLTVLLQDDVGGLHVRREI 270
           LN N L + + T  +    +YYP  P PDL++G+  HSD S LT+LLQD +GGL V  + 
Sbjct: 206 LNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHIGGLQVLHD- 265

Query: 271 DSKGDEWVQVKPVAESLVINIGDFLEIMSNGIYKSVEHCVVSNGNIDRMSIPIFVG---L 330
                 WV V PV  +LV+N+GD L++++N  + SVEH V++N    R+S+  F     +
Sbjct: 266 ----QYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLANVAGPRISVACFFSSYLM 325

Query: 331 QNSPTIGPLPEVLATGKKAEYKEVVFSDYIKYFLNKPLDGKSTLKLAKI 365
            N    GP+ E+L+      Y++   ++Y K++ +K  DG S L   KI
Sbjct: 326 ANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTSGLLYLKI 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN87.3e-9451.01Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S... [more]
G9M9M11.8e-9249.14Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 ... [more]
G9M9M21.8e-9249.43Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 ... [more]
G9M9M02.3e-9249.14Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 ... [more]
G9M9M47.5e-9148.70Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase F6H2-1-1 OS=Ipomo... [more]
Match NameE-valueIdentityDescription
XP_011652189.13.30e-264100.00feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical prot... [more]
KAF9865102.11.42e-12353.89hypothetical protein H0E87_024538 [Populus deltoides][more]
KAE9462342.11.68e-12352.15hypothetical protein C3L33_05761, partial [Rhododendron williamsianum][more]
XP_034923410.12.86e-12353.89feruloyl CoA ortho-hydroxylase F6H1-3-like [Populus alba] >KAG6792990.1 hypothet... [more]
XP_024449267.11.15e-12253.89feruloyl CoA ortho-hydroxylase 1-like [Populus trichocarpa] >PNS22122.1 hypothet... [more]
Match NameE-valueIdentityDescription
A0A0A0LFH91.60e-264100.00Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6A4LYV68.15e-12452.15Fe2OG dioxygenase domain-containing protein (Fragment) OS=Rhododendron williamsi... [more]
A0A4U5QC141.38e-12353.89Oxidoreductase family protein OS=Populus alba OX=43335 GN=D5086_0000108930 PE=3 ... [more]
A0A2K1R4B15.58e-12353.89Fe2OG dioxygenase domain-containing protein OS=Populus trichocarpa OX=3694 GN=PO... [more]
A0A2N9GN502.25e-12253.89Fe2OG dioxygenase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_L... [more]
Match NameE-valueIdentityDescription
AT3G13610.15.2e-9551.012-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G55290.11.8e-8746.692-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G12900.11.9e-6840.122-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G12270.12.3e-6639.202-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G06620.12.9e-4533.522-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 84..101
score: 48.48
coord: 303..321
score: 37.32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..24
NoneNo IPR availablePANTHERPTHR10209:SF764OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE-RELATEDcoord: 26..356
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 26..356
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 32..356
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 23..347
e-value: 1.0E-83
score: 283.7
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 217..313
e-value: 6.1E-23
score: 81.2
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 70..165
e-value: 8.8E-17
score: 61.9
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 208..315
score: 12.775874

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G035320.2CsGy3G035320.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity