CsGy3G027220 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G027220
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionHydroxymethylbilane synthase
LocationGy14Chr3: 28338997 .. 28341959 (-)
RNA-Seq ExpressionCsGy3G027220
SyntenyCsGy3G027220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAATCCTATCCGCCAACGCAAATGCAGACATTTGCCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATTTCCCCTTTTTGCTTTCTTTCTTTCTTCTTCTTCCCCTCCTCCTCCTCCTCCTCATCTCTCTCAATCTCAAACAAACACTCTCTCAAATGGGTGTTTTATCTTCCCCTTCTGTAAGCCACTCTCCAATGCCTCGCCCTTGTAATGTTGGTTCTCTTTCTCTTCTTGGGTTCTCTTCACTTTCCCTTAAACCTCCGACTTTCTCTAATGGCGCCAAGAAGTTTCATGGCGTTGGTCTCATAAGAGCTGTTGCTGCGGAGCAGCAGGTAGAGAAGACCAAGGTTGCTCTTCTCAGAATTGGCACCAGAGGAAGGTAACTTTTTTGTTTTGTGCGGCTTTTTCAGATTCTCTTTTGCTTTATAGTTTTGGGTTGACTTAAGTTTCTAATTTTGAGTTTTTGGGTGATTTGAGTTTGAAAATGTGGAGGATTGAATTATTGTTGAAGGGTTTGGATCAGTTCAATTTGTGTCTTTCAATTGGGATATAATTTTGCTGTATTGTAGAATTGTTAGGAAGTACAGTATGTTTCTCGTGTTTGTACTTGAATCTATCAAAACTTGTATGTTTACGTTTGTCCAAATGAAGTAGTATGGAAGTATTTTTTGTGTGCTTGTAAATCAGACCATGAAGAACACGAAAGGGTGGAAAATTGAACAAAATTAAAGAAAGTAATAGATTCCTGAGAACTATCAATTGTGGAGAACTTCCTTCCTTACGAATGTGCTTAACTTCTGAATTGATTTAATTCGGATAAAGTATTCATGTTGCTCATGCCATCAAATTTAGGATCCAATCTGCTGCTGTTAGAGTAGAACGAGTTCTTCATAAGAAGAGTAAGTCTAAGAGGGGGTTCGAATTCAAAGTTTTCACGTTTATCTTTGGATTTCATGTTTCTTCATTTTCTAAGTTTCATGTTTCTCCTTAGGCTTTCGAGTTGTTTTTTGTGACTGTATAGGTAGTATTTTGCTTGATTGCTCCTTTTTCCTAGAAGGTCCCCTCTTTTGTAGGCTTGGTTTTTTATTTCATTTTTTCTAAAAGAAAACAGAAAGTAAAAAAAAAAAAGTTTATTCTAGTAGGAGTTAGGGGGACATTTAATTTTCTTGTTGAAACCGTGAACTATGTTGATAAACAGTCTACTTCATTTTCTATATGCTTGTTTGAGCTAAATCTTCTTTTGGGTTGCTTCATCTTCTTCTGTGTCATATATAATTTTCTGTGTGTAGGACAGCCCATTAGCACTTGCCCAGGCTCATGAGACGAGAGACAAACTCATGGCTTCCCATCCTGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTGTAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCAACTTACTTGCCTGAAAAAACCATCCTCCCATGTAACCTTCCGAGGGAGGATGTCCGCGATGCATTCATTTCTCTGAGTGCAGGTTCATTTGCTGAGCTTCCAGCTGGAAGCATCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTAAGTATGCTATGGTTCTACGTTTGCTAGGTTGTTGATCGCAGTGACTGATGTGTGAAATAAGCTCCTAGAAAATATGATAGAAAGAATGTCTCTTTGGATTTCGTTCTAAATTGGTTGATCCATAAACCCATAAACTCCATTGCAGTTGTGTTGTTTATGTTCTCAATAAATTCCAACCTTTTCCTTCTTAGTCATTATAATACAATGGGGTGTCATAATTGTTTGACAACGATAGTTTATTTTCTTATTTGAGATTGGAAGATTCCCTGCTGCTCCCATCTGTCCATTCTTTTAATCTAGAATGCCCAACTCATGATGCAACATTTACGACTATGATTTTAAAGATGTTTGAAGAGGATTAAAATCTCATTCTCTTAGATTGATTCTGACACTCTCTCTCGCATTGGTGTCATCTGTTAGGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTGTTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAATGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATTGGGATTGCCTGTCGAAGTGACGATGACATAATGGTGTGTTTTCATTAGCTATCGAAATTGATGACCCATGTTCGTCTGGCAATTGCCATTATAACTATTATCTTGTTGAGAAAACCATCGTTCCTATTCAAGTGGCTGACTTCTTATCTCCGTATAGGCTTATACGATTAAAATTCATTGCTTGGAAAAATGCTTAATCTCTCTCTTGTTTGATTTCTCATTGTGCAGGTCAATTACTTAGCCTCGTTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTTCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATAGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGCTGGTGGCTTCCCCAGATGGAACCCGAGGTACTACGATCAACATGCATGTTCATATGAAATAACTTCTACCCTTGTTTTCGTTAGCTTCTTCTCTCCATTGATAGACCATTGGTCATCTTCATCTTTTGATCCTTACCCTTGTTTCTTCCCTTTCATTTCAGTTCTTGAAACTTCTCGACGAGGTCCGTACGCCATTGAAGATATGATTGCAATGGGAAAGGATGCTGGCCAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

mRNA sequence

ATGAGAATCCTATCCGCCAACGCAAATGCAGACATTTGCCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATTTCCCCTTTTTGCTTTCTTTCTTTCTTCTTCTTCCCCTCCTCCTCCTCCTCCTCATCTCTCTCAATCTCAAACAAACACTCTCTCAAATGGGTGTTTTATCTTCCCCTTCTGTAAGCCACTCTCCAATGCCTCGCCCTTGTAATGTTGGTTCTCTTTCTCTTCTTGGGTTCTCTTCACTTTCCCTTAAACCTCCGACTTTCTCTAATGGCGCCAAGAAGTTTCATGGCGTTGGTCTCATAAGAGCTGTTGCTGCGGAGCAGCAGGTAGAGAAGACCAAGGTTGCTCTTCTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACGAGAGACAAACTCATGGCTTCCCATCCTGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTGTAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCAACTTACTTGCCTGAAAAAACCATCCTCCCATGTAACCTTCCGAGGGAGGATGTCCGCGATGCATTCATTTCTCTGAGTGCAGGTTCATTTGCTGAGCTTCCAGCTGGAAGCATCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTGTTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAATGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATTGGGATTGCCTGTCGAAGTGACGATGACATAATGGTCAATTACTTAGCCTCGTTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTTCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATAGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGCTGGTGGCTTCCCCAGATGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTCCGTACGCCATTGAAGATATGATTGCAATGGGAAAGGATGCTGGCCAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

Coding sequence (CDS)

ATGAGAATCCTATCCGCCAACGCAAATGCAGACATTTGCCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATTTCCCCTTTTTGCTTTCTTTCTTTCTTCTTCTTCCCCTCCTCCTCCTCCTCCTCATCTCTCTCAATCTCAAACAAACACTCTCTCAAATGGGTGTTTTATCTTCCCCTTCTGTAAGCCACTCTCCAATGCCTCGCCCTTGTAATGTTGGTTCTCTTTCTCTTCTTGGGTTCTCTTCACTTTCCCTTAAACCTCCGACTTTCTCTAATGGCGCCAAGAAGTTTCATGGCGTTGGTCTCATAAGAGCTGTTGCTGCGGAGCAGCAGGTAGAGAAGACCAAGGTTGCTCTTCTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACGAGAGACAAACTCATGGCTTCCCATCCTGAGCTAGCCGAAGATGGGGCCATTCAGATTGTTGTAATCAAAACAACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACTAAAGAGATCGATGATGCACTTATTAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTACCAACTTACTTGCCTGAAAAAACCATCCTCCCATGTAACCTTCCGAGGGAGGATGTCCGCGATGCATTCATTTCTCTGAGTGCAGGTTCATTTGCTGAGCTTCCAGCTGGAAGCATCATTGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATAGATATCCATCCCTCAAAGTGCTGGAGAATTTTCGGGGCAATGTCCAAACAAGGTTGAGAAAACTGAATGAAGGAGTGGTCCAAGCAACACTGTTGGCTCTAGCTGGACTTCGGCGTTTAAATATGACAGAAAATGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATTGGGATTGCCTGTCGAAGTGACGATGACATAATGGTCAATTACTTAGCCTCGTTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTTCTTGAGACTCTTGATGGATCTTGCCGAACTCCGATAGCAGGATATGCTTCCAGAGATGAAGATGGCAATTGTATATTTAAAGGGCTGGTGGCTTCCCCAGATGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTCCGTACGCCATTGAAGATATGATTGCAATGGGAAAGGATGCTGGCCAGGAGCTTCTTTCTCGAGCAGGTCCCGGTTTTTTTGATAGCTAG

Protein sequence

MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS*
Homology
[Close]
BLAST of CsGy3G027220 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 7.9e-156
Identity = 291/386 (75.39%), Postives = 322/386 (83.42%), Query Frame = 0 

Query: 50  LKQTLSQMGVLSSPSVSHSPMPRPCNVGSLSL----LGFSSLSLKPPTFSNGAKKFHGVG 109
           ++ TL      S PS   +P        SLSL      FSS    P         F    
Sbjct: 1   MEMTLYSSSSFSLPSAPSNP--------SLSLFTSSFRFSSFKTSP---------FSKCR 60

Query: 110 LIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 169
           +  ++A EQQ ++ K AL+RIGTRGSPLALAQAHETRDKLMASH ELAE+GAIQIV+IKT
Sbjct: 61  IRASLAVEQQTQQNKTALIRIGTRGSPLALAQAHETRDKLMASHTELAEEGAIQIVIIKT 120

Query: 170 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 229
           TGDKILSQPLADIGGKGLFTKEID+ALINGDIDIAVHSMKDVPTYLPE+TILPCNLPRED
Sbjct: 121 TGDKILSQPLADIGGKGLFTKEIDEALINGDIDIAVHSMKDVPTYLPEETILPCNLPRED 180

Query: 230 VRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEG 289
           VRDAFISLSA S A+LPAGS+IGTASLRRKSQ+L+RYPSL V +NFRGNVQTRLRKL+EG
Sbjct: 181 VRDAFISLSAASLADLPAGSVIGTASLRRKSQILHRYPSLTVQDNFRGNVQTRLRKLSEG 240

Query: 290 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHE 349
           VV+ATLLALAGL+RLNMTENVTS LSID+MLPAVAQGAIGIACRS+DD M  YLASLNHE
Sbjct: 241 VVKATLLALAGLKRLNMTENVTSTLSIDDMLPAVAQGAIGIACRSNDDKMAEYLASLNHE 300

Query: 350 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYA 409
           ETRLA+ CERAFL TLDGSCRTPIAGYASRD+DGNC+F+GLVASPDGTRVLETSR G Y 
Sbjct: 301 ETRLAISCERAFLTTLDGSCRTPIAGYASRDKDGNCLFRGLVASPDGTRVLETSRIGSYT 360

Query: 410 IEDMIAMGKDAGQELLSRAGPGFFDS 432
            EDM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 361 YEDMMKIGKDAGEELLSRAGPGFFNS 369

[Close]
BLAST of CsGy3G027220 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 8.8e-155
Identity = 274/356 (76.97%), Postives = 309/356 (86.80%), Query Frame = 0 

Query: 74  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPL 133
           C++GS+S +GFS   +  P      +K    G ++A  A +Q  KT+ A++RIGTRGSPL
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ--KTRTAIIRIGTRGSPL 86

Query: 134 ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 193
           ALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 87  ALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALI 146

Query: 194 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 253
           NG IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A + AELPAGS++GTASLR
Sbjct: 147 NGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLR 206

Query: 254 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 313
           RKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+D
Sbjct: 207 RKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLD 266

Query: 314 EMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 373
           EMLPAVAQGAIGIACR+DDD M  YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYA
Sbjct: 267 EMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYA 326

Query: 374 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFF 430
           S+DE+GNCIF+GLVASPDGT+VLETSR+GPY  EDM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 SKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

[Close]
BLAST of CsGy3G027220 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.6e-140
Identity = 249/325 (76.62%), Postives = 283/325 (87.08%), Query Frame = 0 

Query: 107 IRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTT 166
           +RA  A Q   + KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKTT
Sbjct: 33  VRAAVAVQAEAQAKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKTT 92

Query: 167 GDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDV 226
           GD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPREDV
Sbjct: 93  GDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPREDV 152

Query: 227 RDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGV 286
           RDAFI L+A S AELPAGS++G+ASLRR+SQ+L +YPSLKV+ NFRGNVQTRLRKL EG 
Sbjct: 153 RDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEGD 212

Query: 287 VQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEE 346
           V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M+NYL+SLNHE+
Sbjct: 213 VHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHED 272

Query: 347 TRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI 406
           TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR GPY  
Sbjct: 273 TRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYDF 332

Query: 407 EDMIAMGKDAGQELLSRAGPGFFDS 432
           + M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 DIMVEMGKDAGHELKAKAGPGFFDS 356

[Close]
BLAST of CsGy3G027220 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.5e-85
Identity = 173/307 (56.35%), Postives = 218/307 (71.01%), Query Frame = 0 

Query: 124 LRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 183
           +RIGTRGS LALAQAHETRD+LMA+H  LA D A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 184 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPA 243
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    + +ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 244 GSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMT 303
           G+++G++SLRR++QL  R P LK++E FRGNVQTRL+KL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 304 ENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDG 363
                 +  DEMLPAVAQG IG+  R+DD    + LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 364 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSR 423
           SC+TPIAG A    D     +G +  PDG+ V+   R GP A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 424 AGPGFFD 431
           A   FFD
Sbjct: 311 APADFFD 311

[Close]
BLAST of CsGy3G027220 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 6.2e-84
Identity = 174/316 (55.06%), Postives = 214/316 (67.72%), Query Frame = 0 

Query: 114 QQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 173
           Q  ++T + L  IGTRGSPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 174 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 233
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 234 SAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 293
            AGS  +LPAG+ +GTASLRR++ +L   P LKV  + RGNV+TRLRK++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 294 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVC 353
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD +  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 354 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMG 413
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G  A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 414 KDAGQELLSRAGPGFF 430
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

[Close]
BLAST of CsGy3G027220 vs. NCBI nr
Match: KGN58748.2 (hypothetical protein Csa_002701 [Cucumis sativus])

HSP 1 Score: 823 bits (2125), Expect = 4.86e-300
Identity = 429/431 (99.54%), Postives = 429/431 (99.54%), Query Frame = 0 

Query: 1   MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVL 60
           MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVL
Sbjct: 1   MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVL 60

Query: 61  SSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTK 120
           SSPSVSHSPMPRPCNVGSLS LGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTK
Sbjct: 61  SSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTK 120

Query: 121 VALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGG 180
           VALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGG
Sbjct: 121 VALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGG 180

Query: 181 KGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAE 240
           KGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAE
Sbjct: 181 KGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAE 240

Query: 241 LPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRL 300
           LPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRL
Sbjct: 241 LPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRL 300

Query: 301 NMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLET 360
           NMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIM NYLASLNHEETRLAVVCERAFLET
Sbjct: 301 NMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLET 360

Query: 361 LDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQEL 420
           LDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQEL
Sbjct: 361 LDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQEL 420

Query: 421 LSRAGPGFFDS 431
           LSRAGPGFFDS
Sbjct: 421 LSRAGPGFFDS 431

[Close]
BLAST of CsGy3G027220 vs. NCBI nr
Match: XP_004149732.1 (porphobilinogen deaminase, chloroplastic [Cucumis sativus])

HSP 1 Score: 726 bits (1875), Expect = 7.30e-263
Identity = 373/375 (99.47%), Postives = 373/375 (99.47%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 116
           MGVLSSPSVSHSPMPRPCNVGSLS LGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV
Sbjct: 1   MGVLSSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 60

Query: 117 EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 176
           EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA
Sbjct: 61  EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 120

Query: 177 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 236
           DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG
Sbjct: 121 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 180

Query: 237 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 296
           SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG
Sbjct: 181 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 240

Query: 297 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERA 356
           LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIM NYLASLNHEETRLAVVCERA
Sbjct: 241 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA 300

Query: 357 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 416
           FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA
Sbjct: 301 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 360

Query: 417 GQELLSRAGPGFFDS 431
           GQELLSRAGPGFFDS
Sbjct: 361 GQELLSRAGPGFFDS 375

[Close]
BLAST of CsGy3G027220 vs. NCBI nr
Match: KAG6581556.1 (Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 715 bits (1845), Expect = 1.22e-257
Identity = 377/432 (87.27%), Postives = 388/432 (89.81%), Query Frame = 0 

Query: 1   MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVL 60
           MRILSANANA+I PVGSSKTIPSKLTH  FL S                   + SQM  L
Sbjct: 1   MRILSANANAEIWPVGSSKTIPSKLTHLLFLSS-----------------SSSSSQMVAL 60

Query: 61  SSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKT 120
           SSPSVSHSPM  PCN+ SLSL GFSSLSLKPP F+NG KK HG GLIRA VA EQQ  KT
Sbjct: 61  SSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKT 120

Query: 121 KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG 180
           KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG
Sbjct: 121 KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG 180

Query: 181 GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFA 240
           GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL AGS A
Sbjct: 181 GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLA 240

Query: 241 ELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRR 300
           ELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLN+GVVQATLLALAGLRR
Sbjct: 241 ELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRR 300

Query: 301 LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLE 360
           LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD M  YLASLNHEETRLAVVCERAFLE
Sbjct: 301 LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLE 360

Query: 361 TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQE 420
           TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKDAG+E
Sbjct: 361 TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKE 415

Query: 421 LLSRAGPGFFDS 431
           LLSRAGPGFFDS
Sbjct: 421 LLSRAGPGFFDS 415

[Close]
BLAST of CsGy3G027220 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 702 bits (1811), Expect = 4.26e-253
Identity = 363/376 (96.54%), Postives = 367/376 (97.61%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116
           MGVLSSPSVSHSPMPRP NVGSLSLLGFSSLSLKPP FSNGAKKFHG GLIRA VA EQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176
            EKTKVAL+RIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296
           GS AELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCER 356
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACR+DDD M NYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVCER 300

Query: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 417 AGQELLSRAGPGFFDS 431
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]
BLAST of CsGy3G027220 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687 bits (1773), Expect = 4.94e-247
Identity = 360/408 (88.24%), Postives = 370/408 (90.69%), Query Frame = 0 

Query: 25  LTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVLSSPSVSHSPMPRPCNVGSLSLLGF 84
           LTHFP                IS +L   LS+MGV SSPS+SH PM RPCN+GSLS  GF
Sbjct: 2   LTHFP----------------ISSHLPLPLSEMGVFSSPSISHYPMARPCNLGSLSRHGF 61

Query: 85  SSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVALLRIGTRGSPLALAQAHETRD 144
           SSLSLKPP FS GAKKFHG GLIRA VA EQQ EKTKVAL+RIGTRGSPLALAQAHETR+
Sbjct: 62  SSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQAEKTKVALVRIGTRGSPLALAQAHETRE 121

Query: 145 KLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHS 204
           KLMA HPELAEDGAIQIV+IKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHS
Sbjct: 122 KLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHS 181

Query: 205 MKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYP 264
           MKDVPTYLPEKTILPCNLPREDVRDAFISLS GS AELPAGS+IGTASLRRKSQLLNRYP
Sbjct: 182 MKDVPTYLPEKTILPCNLPREDVRDAFISLSVGSLAELPAGSVIGTASLRRKSQLLNRYP 241

Query: 265 SLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGA 324
           SL VLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTE VTSILSIDEMLPAVAQGA
Sbjct: 242 SLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTETVTSILSIDEMLPAVAQGA 301

Query: 325 IGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIF 384
           IGIACRSDDD M NYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIF
Sbjct: 302 IGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIF 361

Query: 385 KGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 431
           KGLVASPDGTRVLETSRRGPYAI+DMIAMGKDAG+ELLSRAGPGFFDS
Sbjct: 362 KGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGKELLSRAGPGFFDS 393

[Close]
BLAST of CsGy3G027220 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 680 bits (1754), Expect = 9.93e-245
Identity = 349/376 (92.82%), Postives = 357/376 (94.95%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPP FS G KKFHG GLIRA VA EQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176
            EKTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296
           GS AELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCER 356
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD M NYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 417 AGQELLSRAGPGFFDS 431
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]
BLAST of CsGy3G027220 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 679 bits (1753), Expect = 1.41e-244
Identity = 348/376 (92.55%), Postives = 358/376 (95.21%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116
           MGV SSPS+SH PM RPCN+GSLS  GFSSLSLKPP FS G K+FHGVGLIRA VA EQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176
            EKTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS 
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296
           GS AELPAGS+IGTASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCER 356
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDD M NYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 417 AGQELLSRAGPGFFDS 431
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]
BLAST of CsGy3G027220 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 677 bits (1747), Expect = 1.16e-243
Identity = 350/376 (93.09%), Postives = 359/376 (95.48%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116
           M  LSSPSVS+SPM RPCN+ SLSL GFSSLSLKPP F+N  KKFHG GLIRA VA EQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176
            EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296
           GS AELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCER 356
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD M NYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 417 AGQELLSRAGPGFFDS 431
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]
BLAST of CsGy3G027220 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 674 bits (1738), Expect = 2.71e-242
Identity = 348/376 (92.55%), Postives = 356/376 (94.68%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116
           M  LSSPSVSHSPM  PCN+ SLSL GFSSLSLKPP F+NG KK HG GLIRA VA EQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176
             KTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296
           GS AELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCER 356
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD M  YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 417 AGQELLSRAGPGFFDS 431
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]
BLAST of CsGy3G027220 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 672 bits (1735), Expect = 7.77e-242
Identity = 343/376 (91.22%), Postives = 358/376 (95.21%), Query Frame = 0 

Query: 57  MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQ 116
           MGV SSP ++HSPM RPCNVGSLS LGFSSLSLKPP F NG KKF G+GL+RA VA EQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 117 VEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 176
            EKTKVAL+RIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 177 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 236
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 237 GSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 296
           GS AELPAGS+IGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 297 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCER 356
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DD M NYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 357 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKD 416
           AFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAI+DM+AMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 417 AGQELLSRAGPGFFDS 431
           AG+ELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]
BLAST of CsGy3G027220 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 548.1 bits (1411), Expect = 6.2e-156
Identity = 274/356 (76.97%), Postives = 309/356 (86.80%), Query Frame = 0 

Query: 74  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPL 133
           C++GS+S +GFS   +  P      +K    G ++A  A +Q  KT+ A++RIGTRGSPL
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ--KTRTAIIRIGTRGSPL 86

Query: 134 ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 193
           ALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 87  ALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALI 146

Query: 194 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 253
           NG IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A + AELPAGS++GTASLR
Sbjct: 147 NGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLR 206

Query: 254 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 313
           RKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+D
Sbjct: 207 RKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLD 266

Query: 314 EMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 373
           EMLPAVAQGAIGIACR+DDD M  YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYA
Sbjct: 267 EMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYA 326

Query: 374 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFF 430
           S+DE+GNCIF+GLVASPDGT+VLETSR+GPY  EDM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 SKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:
BLAST of CsGy3G027220 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q430827.9e-15675.39Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S... [more]
Q433168.8e-15576.97Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC... [more]
Q6H6D21.6e-14076.62Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994... [more]
A1B8532.5e-8556.35Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586... [more]
Q3SVF36.2e-8455.06Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1... [more]
BLAST of CsGy3G027220 vs. NCBI nr
Match NameE-valueIdentityDescription
KGN58748.24.86e-30099.54hypothetical protein Csa_002701 [Cucumis sativus][more]
XP_004149732.17.30e-26399.47porphobilinogen deaminase, chloroplastic [Cucumis sativus][more]
KAG6581556.11.22e-25787.27Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_038906286.14.26e-25396.54porphobilinogen deaminase, chloroplastic [Benincasa hispida][more]
XP_023523547.14.94e-24788.24porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
BLAST of CsGy3G027220 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GCC79.93e-24592.82Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ... [more]
A0A6J1KA971.41e-24492.55Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV... [more]
A0A6J1IZW21.16e-24393.09Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV... [more]
A0A6J1F3542.71e-24292.55Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ... [more]
A0A6J1DII97.77e-24291.22Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3... [more]
BLAST of CsGy3G027220 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G08280.16.2e-15676.97hydroxymethylbilane synthase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000860Porphobilinogen deaminasePRINTSPR00151PORPHBDMNASEcoord: 353..370
score: 53.17
coord: 266..283
score: 53.97
coord: 246..263
score: 54.23
coord: 167..187
score: 66.21
coord: 198..217
score: 61.19
IPR000860Porphobilinogen deaminaseTIGRFAMTIGR00212TIGR00212coord: 124..423
e-value: 4.0E-100
score: 332.6
IPR000860Porphobilinogen deaminasePANTHERPTHR11557PORPHOBILINOGEN DEAMINASEcoord: 68..430
IPR000860Porphobilinogen deaminaseHAMAPMF_00260Porphobil_deamcoord: 123..423
score: 34.154881
NoneNo IPR availableGENE3D3.40.190.10coord: 225..325
e-value: 1.6E-81
score: 274.6
NoneNo IPR availableGENE3D3.40.190.10coord: 124..333
e-value: 1.6E-81
score: 274.6
NoneNo IPR availablePANTHERPTHR11557:SF8BNAC02G01240D PROTEINcoord: 68..430
NoneNo IPR availableCDDcd13648PBP2_PBGD_1coord: 123..402
e-value: 0.0
score: 513.883
NoneNo IPR availableSUPERFAMILY53850Periplasmic binding protein-like IIcoord: 124..341
IPR022417Porphobilinogen deaminase, N-terminalPFAMPF01379Porphobil_deamcoord: 124..334
e-value: 2.5E-75
score: 252.6
IPR036803Porphobilinogen deaminase, C-terminal domain superfamilyGENE3D3.30.160.40coord: 344..430
e-value: 4.0E-24
score: 86.8
IPR036803Porphobilinogen deaminase, C-terminal domain superfamilySUPERFAMILY54782Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domaincoord: 343..430
IPR022418Porphobilinogen deaminase, C-terminalPFAMPF03900Porphobil_deamCcoord: 349..421
e-value: 4.5E-15
score: 55.8
IPR022419Porphobilinogen deaminase, dipyrromethane cofactor binding sitePROSITEPS00533PORPHOBILINOGEN_DEAMcoord: 354..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G027220.2CsGy3G027220.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018160 peptidyl-pyrromethane cofactor linkage
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0017157 regulation of exocytosis
biological_process GO:0033014 tetrapyrrole biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0004418 hydroxymethylbilane synthase activity
molecular_function GO:0045159 myosin II binding
molecular_function GO:0019905 syntaxin binding