CsGy3G026530 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G026530
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	E3 ubiquitin-protein ligase RNF144B-like
Location	Gy14Chr3: 27356533 .. 27358502 (-)
RNA-Seq Expression	CsGy3G026530
Synteny	CsGy3G026530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAGGATTGTTTCTTTCTCGGCCGAGGGTACTGATTCGAAGACACATATCAATAAATCATCCACAAAAACCCAATAGTCGTCTTCCTATGATTCATTTCGAAAGGCTTTCCCTTCCCACCATCCCTTAAATTAATTCACCCTTTCAAACCCCCTTCCTCCACCACTTCAAATTTCTCTCTAATGGCCTCTTCTTCCTCTTCTTCTTCTATCCTCCTCCAAAATCTCTCCGATGAAAATTACGCAGAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCCTCTCCAATTAATCCATCCCCAACACCCCACTCTTCTACCTCCCAACTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACCAACAGAGTATGTACACATACATTCTGCACCGCTTGCATCTCAAACCACATCGCTGCAAAGCTTGAGGTTGCAATGGCAGTGAAGTGTCCGGAGCCGAATTGCGGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGGATGCTCTGTTTGAGGCGATGATTCTGAAGTGGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATAATTGATGAAGGGGGTGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTTTGTGCAGAATGTAGAGTGGGATGGCATGGGGAAATGGAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGACAGTGAAACTAGCTGAGAACAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTATGTTGAGAAGACTGTTGGTTGTGTTCATATTGTCTGCAGGTTTGTTCTCTAATTTACCCTTTTACTTTTTGGTTTCTATGCTTCCAATTAATTCTCAACCTAGTTTGTGTTTAAATTGTAGAGCTATATGTGGATGACCTTGTCATGATTCAAAGGGATAGGTTGTATGGGTTTGGTATCTGGGGTTGGGTTAGGACGAAAAACTAAGGGCAGTTAAATCAAATATTAATATAATAGAGAAGAGCATACATAAAATAAGTATGAAGATAAGTTGTATATGGTGCAGTATTTATAATTAAGAATAAGATGCAATTGATGGAGATCTTAATTGAATAATGAAATTTGTAGGTGTGGATCGGATTTTTGCTACAGCTGTGGAGCTAAGTGGGGTGGTGGTCATGCTTGTAAGGCTTCAGCTTATGAACGTACTGTCTATCGACGTTGATTACAACCCATCTTTCATCCATATGCATGAATTCAAAATTGTTAGAATTTCATTTTAATGTTTTAACAGTTGGATTTTAAAACTAAATTTTCAGATTTTATTGAACAGGCATAACTAGATGTTCAATGGTATGTTATGAAATGAAATAAAACAAGATGTTAAAAAGTTTACGTACCAAAGTAAAGAGAAACATCTCTTTTACTTGAAATTGAAAATAGTGATAGAAATGGTCTCCCCATATTTGCATTTGTCCTTTAGAGGACTTGTTTGAGAGGGCAGAAATTTATTATTTGGGGTTGAAGGAATATGGACAGTAAATGGCCAATGTCGCCTTTACTTCTTGATGGGATGGTTATCTTCACTGGAGAAGCGATAAGAATAATTTTTCAGTCCATCAAACAACCAAACATAGGCTGTAATGCAACACCCAGGAAATAAAAAGAAGAAGAATTCTTAAGAATCCATTGAAAGGTATGGTGGATTTGGGTGTGGGATAGATAAAGGATGGGTGTCCGTTTGGGGGATTTGAGAAAAGTAAAAGGGTGTTTATTAGATGGAAAACTGACAAAGGAAGAGGAGAGGGTAGGGAACAGTCCATTCCCATCTTTCTTATCACCAAAAACATTTTATCACTTTCTTTTAATCCCTTTACTTGTTACCATCTAAACCTCACTTA

mRNA sequence

TTAGGATTGTTTCTTTCTCGGCCGAGGGTACTGATTCGAAGACACATATCAATAAATCATCCACAAAAACCCAATAGTCGTCTTCCTATGATTCATTTCGAAAGGCTTTCCCTTCCCACCATCCCTTAAATTAATTCACCCTTTCAAACCCCCTTCCTCCACCACTTCAAATTTCTCTCTAATGGCCTCTTCTTCCTCTTCTTCTTCTATCCTCCTCCAAAATCTCTCCGATGAAAATTACGCAGAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCCTCTCCAATTAATCCATCCCCAACACCCCACTCTTCTACCTCCCAACTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACCAACAGAGTATGTACACATACATTCTGCACCGCTTGCATCTCAAACCACATCGCTGCAAAGCTTGAGGTTGCAATGGCAGTGAAGTGTCCGGAGCCGAATTGCGGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGGATGCTCTGTTTGAGGCGATGATTCTGAAGTGGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATAATTGATGAAGGGGGTGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTTTGTGCAGAATGTAGAGTGGGATGGCATGGGGAAATGGAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGACAGTGAAACTAGCTGAGAACAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTATGTTGAGAAGACTGTTGGTTGTGTTCATATTGTCTGCAGGTGTGGATCGGATTTTTGCTACAGCTGTGGAGCTAAGTGGGGTGGTGGTCATGCTTGTAAGGCTTCAGCTTATGAACGTACTGTCTATCGACGTTGATTACAACCCATCTTTCATCCATATGCATGAATTCAAAATTGTTAGAATTTCATTTTAATGTTTTAACAGTTGGATTTTAAAACTAAATTTTCAGATTTTATTGAACAGGCATAACTAGATGTTCAATGGTATGTTATGAAATGAAATAAAACAAGATGTTAAAAAGTTTACGTACCAAAGTAAAGAGAAACATCTCTTTTACTTGAAATTGAAAATAGTGATAGAAATGGTCTCCCCATATTTGCATTTGTCCTTTAGAGGACTTGTTTGAGAGGGCAGAAATTTATTATTTGGGGTTGAAGGAATATGGACAGTAAATGGCCAATGTCGCCTTTACTTCTTGATGGGATGGTTATCTTCACTGGAGAAGCGATAAGAATAATTTTTCAGTCCATCAAACAACCAAACATAGGCTGTAATGCAACACCCAGGAAATAAAAAGAAGAAGAATTCTTAAGAATCCATTGAAAGGTATGGTGGATTTGGGTGTGGGATAGATAAAGGATGGGTGTCCGTTTGGGGGATTTGAGAAAAGTAAAAGGGTGTTTATTAGATGGAAAACTGACAAAGGAAGAGGAGAGGGTAGGGAACAGTCCATTCCCATCTTTCTTATCACCAAAAACATTTTATCACTTTCTTTTAATCCCTTTACTTGTTACCATCTAAACCTCACTTA

Coding sequence (CDS)

ATGGCCTCTTCTTCCTCTTCTTCTTCTATCCTCCTCCAAAATCTCTCCGATGAAAATTACGCAGAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCCTCTCCAATTAATCCATCCCCAACACCCCACTCTTCTACCTCCCAACTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACCAACAGAGTATGTACACATACATTCTGCACCGCTTGCATCTCAAACCACATCGCTGCAAAGCTTGAGGTTGCAATGGCAGTGAAGTGTCCGGAGCCGAATTGCGGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGGATGCTCTGTTTGAGGCGATGATTCTGAAGTGGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATAATTGATGAAGGGGGTGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTTTGTGCAGAATGTAGAGTGGGATGGCATGGGGAAATGGAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGACAGTGAAACTAGCTGAGAACAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTATGTTGAGAAGACTGTTGGTTGTGTTCATATTGTCTGCAGGTGTGGATCGGATTTTTGCTACAGCTGTGGAGCTAAGTGGGGTGGTGGTCATGCTTGTAAGGCTTCAGCTTATGAACGTACTGTCTATCGACGTTGA

Protein sequence

MASSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAKSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCYSCGAKWGGGHACKASAYERTVYRR*

Homology

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BLAST of CsGy3G026530 vs. ExPASy Swiss-Prot
Match: A4IIY1 (Probable E3 ubiquitin-protein ligase RNF144A OS=Xenopus tropicalis OX=8364 GN=rnf144a PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 55/200 (27.50%), Postives = 93/200 (46.50%), Query Frame = 0


Query: 54  CSICTDAKSHSQMFTNRVCTHTFCTACISNHIAAKLEVAM--AVKCPEPNC---GTVLEP 113
           C +C    +  QM T   C   FCT C+  ++   ++  +  A+ CP+ +C   G + E 
Sbjct: 20  CKLCLGEYTVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDASCPKRGHLQEN 79

Query: 114 EMCGSFVPKRVLERWADALFE-AMILKWKRLNCPFKDCGAAI-IDEGGEEGVTAVECGSC 173
           E+    V   +++++    FE  ++L   R  CP   C A   + E G +    V+C +C
Sbjct: 80  EI-ECMVAAEIMQKYKKLQFEKEILLDPCRTWCPSSSCQAVCKLQEKGIQNPQLVQCSAC 139

Query: 174 WRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEK 233
              FC+ C+  WH    C E   +    G S     +    L ++   +RCP CK+Y+E+
Sbjct: 140 DIEFCSACKANWHPGQGCPENMAITFLPGDS----SSFFKSLEDDVPIKRCPKCKVYIER 199

Query: 234 TVGCVHIVCR-CGSDFCYSC 246
             GC  ++C+ C   FC+ C
Sbjct: 200 DEGCAQMMCKNCKHAFCWYC 214

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BLAST of CsGy3G026530 vs. ExPASy Swiss-Prot
Match: Q7Z419 (E3 ubiquitin-protein ligase RNF144B OS=Homo sapiens OX=9606 GN=RNF144B PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.3e-17
Identity = 62/214 (28.97%), Postives = 98/214 (45.79%), Query Frame = 0


Query: 40  NPSPTPHSSTSQLLCSICTDAKSHSQMFTNRVCTHTFCTACISNH--IAAKLEVAMAVKC 99
           NP+P   +    + C +C   +S  +M T + C   FCTAC+  +  +A +      + C
Sbjct: 16  NPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITC 75

Query: 100 PEPNC---GTVLEPEMCGSFVPKRVLERWADALFEAMI-LKWKRLNCPFKDCGAAIIDEG 159
           P+  C   GT+ E E+    VP    + +    FE  + L   R  CP  DC        
Sbjct: 76  PDMVCLNHGTLQEAEI-ACLVPVDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVAS 135

Query: 160 GEEG-VTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENK 219
            + G    VEC SC   FC+ C+  WH E+ C + Q +     V   +  A+    AE  
Sbjct: 136 SDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPI-----VLPTEHRALFGTDAE-A 195

Query: 220 KWRRCPHCKIYVEKTVGCVHIVCR-CGSDFCYSC 246
             ++CP C++Y+E+  GC  ++C+ C   FC+ C
Sbjct: 196 PIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYC 222

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BLAST of CsGy3G026530 vs. ExPASy Swiss-Prot
Match: A5PK27 (E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 6.9e-17
Identity = 64/217 (29.49%), Postives = 99/217 (45.62%), Query Frame = 0


Query: 40  NPSPTPHSSTSQLLCSICTDAKSHSQMFTNRVCTHTFCTACISNH--IAAKLEVAMAVKC 99
           NP+P   +    + C +C   +S  +M T + C   FCTAC+  +  +A +      + C
Sbjct: 17  NPTPGDLALVPLVTCKLCLCEQSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITC 76

Query: 100 PEPNC---GTVLEPEMCGSFVPKRVLERWADALFEAMI-LKWKRLNCPFKDCGAAIIDEG 159
           P+  C   GT+ E E+    VP    + +    FE  + L   R  CP  DC        
Sbjct: 77  PDMVCLNHGTLQEAEI-ACLVPVDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVAT 136

Query: 160 GEEG-VTAVECGSCWRLFCAECRVGWHGEMECGEFQR--LRKEAG-VSGDKDDAMTVKLA 219
            + G    VEC SC   FC+ C+  WH E+ C + Q   L  E G + G + DA      
Sbjct: 137 SDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPGILPTEHGTLFGTETDAPI---- 196

Query: 220 ENKKWRRCPHCKIYVEKTVGCVHIVCR-CGSDFCYSC 246
                ++CP C++Y+E+  GC  ++C+ C   FC+ C
Sbjct: 197 -----KQCPVCRVYIERNEGCAQMMCKNCKHTFCWYC 223

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BLAST of CsGy3G026530 vs. ExPASy Swiss-Prot
Match: Q8BKD6 (E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 57/200 (28.50%), Postives = 86/200 (43.00%), Query Frame = 0


Query: 54  CSICTDAKSHSQMFTNRVCTHTFCTACISNHIAAKLE--VAMAVKCPEPNC---GTVLEP 113
           C +C   +S  +M   + C   FCT C+  ++   +       + CP+  C   GT+ E 
Sbjct: 30  CKLCLCEQSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCLNHGTLQET 89

Query: 114 EMCGSFVPKRVLERWADALFEAMI-LKWKRLNCPFKDCGAAIIDEGGEEG-VTAVECGSC 173
           E+    VP    + +    FE  + +   R  CP  DC        G+ G    VEC SC
Sbjct: 90  EI-ACLVPLDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECPSC 149

Query: 174 WRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEK 233
              FC+ C+  WH E  C + Q    E G     D    +K        +CP C+IY+E+
Sbjct: 150 HLKFCSCCKDAWHEESSCRDSQSAMPEHGALFGTDADAPIK--------QCPVCRIYIER 209

Query: 234 TVGCVHIVCR-CGSDFCYSC 246
             GC  ++C+ C   FC+ C
Sbjct: 210 NEGCAQMMCKNCKHTFCWYC 220

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BLAST of CsGy3G026530 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.0e-16
Identity = 55/210 (26.19%), Postives = 92/210 (43.81%), Query Frame = 0


Query: 54  CSICTDAKSHSQMFTNRVCTHTFCTACISNHIAAKLE---VAMAVKCPEPNCGTVLEPEM 113
           C IC +    +Q F   +C H +C  C  N++  K+      +   CP P C  ++  + 
Sbjct: 139 CLICLEDYPPTQTFA-LICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 198

Query: 114 CGSFVPKRVLERWADALFEAMI-----LKWKRLNCPFKDCGAAI-IDEGGEEGVTAVECG 173
               V   V ER+ + + ++ +     +KW    CP   C  +I  D    +     +CG
Sbjct: 199 FKQIVSPEVFERFNNFILKSYVDDNPQVKW----CPAPGCIYSIRCDRKERKEAVNCKCG 258

Query: 174 SCWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYV 233
             +   C +  +G H    C +  +  ++A    D+ + +T  LA  KK   CP C+  +
Sbjct: 259 FQYCFNCNDYEIGDHMPCPCSQVDKWLQKA---SDESENVTWMLANTKK---CPECRSPI 318

Query: 234 EKTVGCVHIVCR-----CGSDFCYSCGAKW 250
           EK  GC+H+ CR     CG +FC+ C   W
Sbjct: 319 EKNGGCMHMTCRKNAGGCGFEFCWLCRGPW 337

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BLAST of CsGy3G026530 vs. NCBI nr
Match: XP_004144476.2 (probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypothetical protein Csa_001774 [Cucumis sativus])

HSP 1 Score: 556 bits (1433), Expect = 4.49e-200
Identity = 265/266 (99.62%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 2   ASSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK 61
           +SSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK
Sbjct: 4   SSSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK 63

Query: 62  SHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLE 121
           SHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLE
Sbjct: 64  SHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLE 123

Query: 122 RWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE 181
           RWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE
Sbjct: 124 RWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE 183

Query: 182 MECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDF 241
           MECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDF
Sbjct: 184 MECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDF 243

Query: 242 CYSCGAKWGGGHACKASAYERTVYRR 267
           CYSCGAKWGGGHACKASAYERTVYRR
Sbjct: 244 CYSCGAKWGGGHACKASAYERTVYRR 269

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BLAST of CsGy3G026530 vs. NCBI nr
Match: XP_008460341.1 (PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa] >TYK19145.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 505 bits (1300), Expect = 8.49e-180
Identity = 241/267 (90.26%), Postives = 250/267 (93.63%), Query Frame = 0


Query: 5   SSSSSILLQNLSDENYAEELQLQEVITSSLLSSPI----NPSPTPHSSTSQLLCSICTDA 64
           S SSSI   +LSDENYAEELQLQEVITSSLLS PI    +PSPTPHSSTSQ+LCSICTDA
Sbjct: 3   SPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDA 62

Query: 65  KSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVL 124
           KSHSQMFTNRVCTHTFCT CIS HIAAKLEV MAVKCPEPNCGTVLEPEMCGSFVPKRVL
Sbjct: 63  KSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVL 122

Query: 125 ERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHG 184
           ERWADALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGWHG
Sbjct: 123 ERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHG 182

Query: 185 EMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSD 244
           EM+CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCGS+
Sbjct: 183 EMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSE 242

Query: 245 FCYSCGAKWGGGHACKASAYERTVYRR 267
           FCYSCG KWGG HAC+ SAYERTV+RR
Sbjct: 243 FCYSCGGKWGGAHACRGSAYERTVHRR 269

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BLAST of CsGy3G026530 vs. NCBI nr
Match: XP_038889081.1 (probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida])

HSP 1 Score: 387 bits (993), Expect = 6.29e-132
Identity = 182/264 (68.94%), Postives = 216/264 (81.82%), Query Frame = 0


Query: 6   SSSSILLQNLSDENYAEELQLQEVITSSLLSSPI-----NPSPTPHSSTSQLLCSICTDA 65
           SSSS  L +LSDEN+A ELQLQE ITSSL S+P      N SP P  S    +C+ICTDA
Sbjct: 12  SSSSHFLHDLSDENFAYELQLQEAITSSLSSTPAEIPNNNSSPKPQDS----ICTICTDA 71

Query: 66  KSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVL 125
           K  S+MFTN +C+HTFCT CIS +IAAKLE +MAVKCP+P CGT+LEPEMC SF+PK VL
Sbjct: 72  KLASEMFTNGLCSHTFCTDCISKYIAAKLEDSMAVKCPQPKCGTLLEPEMCRSFIPKEVL 131

Query: 126 ERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHG 185
           ERW DALFEAMILKWKRLNCPFKDC A +I EG E+GVTAVEC +CWRLFCA+C+VGWHG
Sbjct: 132 ERWGDALFEAMILKWKRLNCPFKDCSAPMIYEGDEKGVTAVECLNCWRLFCAQCKVGWHG 191

Query: 186 EMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSD 245
           +MEC EF+++RKE     + +D MT+KLA+ KKW++CP+CK+YVEKTVGCVHI+CRCG+ 
Sbjct: 192 DMECREFEKVRKE-----NTEDMMTMKLADEKKWKKCPNCKVYVEKTVGCVHIICRCGTQ 251

Query: 246 FCYSCGAKWGGGHACKASAYERTV 264
           FCYSCGAKWGGGHAC  SAYE ++
Sbjct: 252 FCYSCGAKWGGGHACPNSAYEHSM 266

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BLAST of CsGy3G026530 vs. NCBI nr
Match: XP_022956145.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata])

HSP 1 Score: 360 bits (925), Expect = 1.72e-122
Identity = 170/256 (66.41%), Postives = 196/256 (76.56%), Query Frame = 0


Query: 15  LSDENYAEELQLQEVITSSLLSSPI---NPSPTPHSSTSQLLCSICTDAKSHSQMFTNRV 74
           +SDE +AEELQLQE I SSL +S I    PSP P SS S  LC+ICTD KS S+MFT+  
Sbjct: 32  ISDEIFAEELQLQESIASSLFTSTIMSNKPSPNPESSGSTTLCTICTDTKSASEMFTSTG 91

Query: 75  CTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAM 134
           C+HTFC  CIS HIAAKLE +MAVKCPEP C  VLEPEMC SFVPK VLERW DAL +AM
Sbjct: 92  CSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKEVLERWGDALLKAM 151

Query: 135 ILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLR 194
           +LKWK + CPFKDC A ++DE   E + AVEC  CWR+FCA+C VGWHG+M C EFQRL 
Sbjct: 152 LLKWKMVYCPFKDCSAGMVDE---EDMEAVECPKCWRMFCAKCGVGWHGDMNCTEFQRLL 211

Query: 195 KEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCYSCGAKWGG 254
           K  GV  D+++ MT+KLA+ +KW+RCPHCKI VEK  GC+HI CRCGS+FCY CGAKW G
Sbjct: 212 KNGGV--DREEEMTMKLADERKWKRCPHCKILVEKIEGCLHITCRCGSEFCYCCGAKWNG 271

Query: 255 GHACKASAYERTVYRR 267
            HAC  SAYERT YRR
Sbjct: 272 HHACPPSAYERTAYRR 282

[Close]

BLAST of CsGy3G026530 vs. NCBI nr
Match: XP_022980686.1 (probable E3 ubiquitin-protein ligase ARI5 [Cucurbita maxima])

HSP 1 Score: 346 bits (888), Expect = 7.00e-117
Identity = 163/255 (63.92%), Postives = 194/255 (76.08%), Query Frame = 0


Query: 15  LSDENYAEELQLQEVITSSLLSSPIN--PSPTPHSSTSQLLCSICTDAKSHSQMFTNRVC 74
           +SDE +AEELQLQE I SS  S+ ++  PSP P SS S+ LC+ICTD KS S+MFT+  C
Sbjct: 32  ISDEIFAEELQLQESIASSFTSTIMSNKPSPNPESSGSKTLCTICTDTKSASEMFTSTGC 91

Query: 75  THTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMI 134
           +HTFC  CIS HIAAKL  ++AVKCPEP C  VL+PEMC SFVPK VLERW DAL +AM+
Sbjct: 92  SHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKEVLERWGDALLKAML 151

Query: 135 LKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRK 194
           LKWK + CPFKDC A ++DE   E   AVEC  CWR+FCA+C VGWH +M C EFQRL K
Sbjct: 152 LKWKMVYCPFKDCSAGMVDEEDRE---AVECPKCWRMFCAKCGVGWHADMNCTEFQRLLK 211

Query: 195 EAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCYSCGAKWGGG 254
             GV  D+++ MT+KLA+ +KW+RCPHCKI VEK +GC+HI CRCGS+FCYSCG KW   
Sbjct: 212 NGGV--DREEEMTMKLADERKWKRCPHCKILVEKILGCLHITCRCGSEFCYSCGGKWNDR 271

Query: 255 HACKASAYERTVYRR 267
           HAC  SAYERTV RR
Sbjct: 272 HACPPSAYERTVNRR 281

[Close]

BLAST of CsGy3G026530 vs. ExPASy TrEMBL
Match: A0A0A0L952 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 2.17e-200
Identity = 265/266 (99.62%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 2   ASSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK 61
           +SSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK
Sbjct: 4   SSSSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK 63

Query: 62  SHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLE 121
           SHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLE
Sbjct: 64  SHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLE 123

Query: 122 RWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE 181
           RWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE
Sbjct: 124 RWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE 183

Query: 182 MECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDF 241
           MECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDF
Sbjct: 184 MECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDF 243

Query: 242 CYSCGAKWGGGHACKASAYERTVYRR 267
           CYSCGAKWGGGHACKASAYERTVYRR
Sbjct: 244 CYSCGAKWGGGHACKASAYERTVYRR 269

[Close]

BLAST of CsGy3G026530 vs. ExPASy TrEMBL
Match: A0A5A7UED3 (E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00700 PE=3 SV=1)

HSP 1 Score: 505 bits (1300), Expect = 4.11e-180
Identity = 241/267 (90.26%), Postives = 250/267 (93.63%), Query Frame = 0


Query: 5   SSSSSILLQNLSDENYAEELQLQEVITSSLLSSPI----NPSPTPHSSTSQLLCSICTDA 64
           S SSSI   +LSDENYAEELQLQEVITSSLLS PI    +PSPTPHSSTSQ+LCSICTDA
Sbjct: 3   SPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDA 62

Query: 65  KSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVL 124
           KSHSQMFTNRVCTHTFCT CIS HIAAKLEV MAVKCPEPNCGTVLEPEMCGSFVPKRVL
Sbjct: 63  KSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVL 122

Query: 125 ERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHG 184
           ERWADALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGWHG
Sbjct: 123 ERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHG 182

Query: 185 EMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSD 244
           EM+CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCGS+
Sbjct: 183 EMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSE 242

Query: 245 FCYSCGAKWGGGHACKASAYERTVYRR 267
           FCYSCG KWGG HAC+ SAYERTV+RR
Sbjct: 243 FCYSCGGKWGGAHACRGSAYERTVHRR 269

[Close]

BLAST of CsGy3G026530 vs. ExPASy TrEMBL
Match: A0A1S3CDI0 (E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194 PE=3 SV=1)

HSP 1 Score: 505 bits (1300), Expect = 4.11e-180
Identity = 241/267 (90.26%), Postives = 250/267 (93.63%), Query Frame = 0


Query: 5   SSSSSILLQNLSDENYAEELQLQEVITSSLLSSPI----NPSPTPHSSTSQLLCSICTDA 64
           S SSSI   +LSDENYAEELQLQEVITSSLLS PI    +PSPTPHSSTSQ+LCSICTDA
Sbjct: 3   SPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICTDA 62

Query: 65  KSHSQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVL 124
           KSHSQMFTNRVCTHTFCT CIS HIAAKLEV MAVKCPEPNCGTVLEPEMCGSFVPKRVL
Sbjct: 63  KSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKRVL 122

Query: 125 ERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHG 184
           ERWADALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGWHG
Sbjct: 123 ERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGWHG 182

Query: 185 EMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSD 244
           EM+CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCGS+
Sbjct: 183 EMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCGSE 242

Query: 245 FCYSCGAKWGGGHACKASAYERTVYRR 267
           FCYSCG KWGG HAC+ SAYERTV+RR
Sbjct: 243 FCYSCGGKWGGAHACRGSAYERTVHRR 269

[Close]

BLAST of CsGy3G026530 vs. ExPASy TrEMBL
Match: A0A6J1GX07 (probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC111457924 PE=3 SV=1)

HSP 1 Score: 360 bits (925), Expect = 8.32e-123
Identity = 170/256 (66.41%), Postives = 196/256 (76.56%), Query Frame = 0


Query: 15  LSDENYAEELQLQEVITSSLLSSPI---NPSPTPHSSTSQLLCSICTDAKSHSQMFTNRV 74
           +SDE +AEELQLQE I SSL +S I    PSP P SS S  LC+ICTD KS S+MFT+  
Sbjct: 32  ISDEIFAEELQLQESIASSLFTSTIMSNKPSPNPESSGSTTLCTICTDTKSASEMFTSTG 91

Query: 75  CTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAM 134
           C+HTFC  CIS HIAAKLE +MAVKCPEP C  VLEPEMC SFVPK VLERW DAL +AM
Sbjct: 92  CSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKEVLERWGDALLKAM 151

Query: 135 ILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLR 194
           +LKWK + CPFKDC A ++DE   E + AVEC  CWR+FCA+C VGWHG+M C EFQRL 
Sbjct: 152 LLKWKMVYCPFKDCSAGMVDE---EDMEAVECPKCWRMFCAKCGVGWHGDMNCTEFQRLL 211

Query: 195 KEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCYSCGAKWGG 254
           K  GV  D+++ MT+KLA+ +KW+RCPHCKI VEK  GC+HI CRCGS+FCY CGAKW G
Sbjct: 212 KNGGV--DREEEMTMKLADERKWKRCPHCKILVEKIEGCLHITCRCGSEFCYCCGAKWNG 271

Query: 255 GHACKASAYERTVYRR 267
            HAC  SAYERT YRR
Sbjct: 272 HHACPPSAYERTAYRR 282

[Close]

BLAST of CsGy3G026530 vs. ExPASy TrEMBL
Match: A0A6J1IU95 (probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC111479983 PE=3 SV=1)

HSP 1 Score: 346 bits (888), Expect = 3.39e-117
Identity = 163/255 (63.92%), Postives = 194/255 (76.08%), Query Frame = 0


Query: 15  LSDENYAEELQLQEVITSSLLSSPIN--PSPTPHSSTSQLLCSICTDAKSHSQMFTNRVC 74
           +SDE +AEELQLQE I SS  S+ ++  PSP P SS S+ LC+ICTD KS S+MFT+  C
Sbjct: 32  ISDEIFAEELQLQESIASSFTSTIMSNKPSPNPESSGSKTLCTICTDTKSASEMFTSTGC 91

Query: 75  THTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMI 134
           +HTFC  CIS HIAAKL  ++AVKCPEP C  VL+PEMC SFVPK VLERW DAL +AM+
Sbjct: 92  SHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKEVLERWGDALLKAML 151

Query: 135 LKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRK 194
           LKWK + CPFKDC A ++DE   E   AVEC  CWR+FCA+C VGWH +M C EFQRL K
Sbjct: 152 LKWKMVYCPFKDCSAGMVDEEDRE---AVECPKCWRMFCAKCGVGWHADMNCTEFQRLLK 211

Query: 195 EAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCYSCGAKWGGG 254
             GV  D+++ MT+KLA+ +KW+RCPHCKI VEK +GC+HI CRCGS+FCYSCG KW   
Sbjct: 212 NGGV--DREEEMTMKLADERKWKRCPHCKILVEKILGCLHITCRCGSEFCYSCGGKWNDR 271

Query: 255 HACKASAYERTVYRR 267
           HAC  SAYERTV RR
Sbjct: 272 HACPPSAYERTVNRR 281

[Close]

BLAST of CsGy3G026530 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 3.6e-61
Identity = 112/266 (42.11%), Postives = 152/266 (57.14%), Query Frame = 0


Query: 15  LSDENYAEELQLQEVITSSLLSSP--INPSPTPHSS-------------------TSQLL 74
           ++DE+YA E+ LQE + SS  +S   +N  P  H +                    S+ L
Sbjct: 35  IADESYATEIHLQEAMFSSFGASTVGVNHHPQVHRNLVTLVKQEPEIKAENEPMEPSRRL 94

Query: 75  CSICTDAKSHSQMFTNRV-CTHTFCTACISNHIAAKL-EVAMAVKCPEPNCGTVLEPEMC 134
           C IC D K  S +F     CTH +CT C   ++A K+ E A  +KCP+  C  ++EP  C
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTC 154

Query: 135 GSFVPKRVLERWADALFEAMILKWKRLNCPFKDCGAAII-DEGGEEGVTAVECGSCWRLF 194
              +PK V +RW   L E++I  W +  CPFKDC A ++ +E G+  VT  EC SC RLF
Sbjct: 155 RDLIPKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLF 214

Query: 195 CAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGC 254
           C +C+V WH  + C EFQR       S D+DDA+ +++A+NK+WRRCP CK YV+K  GC
Sbjct: 215 CVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEGC 274

Query: 255 VHIVCRCGSDFCYSCGAKWGGGHACK 257
            HI CRCG  FCY CG+ W   HAC+
Sbjct: 275 QHIKCRCGYQFCYGCGSVWSSSHACQ 300

[Close]

BLAST of CsGy3G026530 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 3.7e-50
Identity = 87/201 (43.28%), Postives = 123/201 (61.19%), Query Frame = 0


Query: 47  SSTSQLLCSICTDAKSHSQMFTNRVCTHTFCTACISNHIAAKL-EVAMAVKCPEPNCGTV 106
           S T+   C IC D+KS  + F    C+H +C  C+S +IAAKL +  ++++CP   C   
Sbjct: 108 SKTATFDCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGR 167

Query: 107 LEPEMCGSFVPKRVLERWADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGS 166
           LEP+ C   +PK V +RW DAL EA++++ K+  CP+KDC A +  E  E  +   EC  
Sbjct: 168 LEPDQCRQILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPH 227

Query: 167 CWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVE 226
           C R+ C EC   WH EM C EFQ+L   A     +DD +   +A+ KKW+RCP CK Y+E
Sbjct: 228 CHRMVCVECGTQWHPEMTCEEFQKL---AANERGRDDILLATMAKQKKWKRCPSCKFYIE 287

Query: 227 KTVGCVHIVCRCGSDFCYSCG 247
           K+ GC+++ CRCG  FCY+CG
Sbjct: 288 KSQGCLYMKCRCGLAFCYNCG 305

[Close]

BLAST of CsGy3G026530 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 145.6 bits (366), Expect = 5.8e-35
Identity = 81/256 (31.64%), Postives = 120/256 (46.88%), Query Frame = 0


Query: 6   SSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLL--CSICTDAKSH 65
           SS   +L   +D  +A +L  + ++      S + P   P  + + L   C+IC +    
Sbjct: 257 SSYQHVLVARNDVKFAYKLARESIL------SMVTPHEDPRQAKAVLKEECAICFNDIVA 316

Query: 66  SQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERW 125
             MF+   C H FC  C+  H+  KL   MA KCP   C + L  + CG  +  ++ + W
Sbjct: 317 EGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSELVIDACGKLLTPKLSKLW 376

Query: 126 ADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAV----------ECGSCWRLFCAE 185
              L E  I   +R+ CP+  C A +      E   ++           C  C  LFC +
Sbjct: 377 QQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVD 436

Query: 186 CRVGWHGEMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHI 245
           C+V WHG + C E+++L  E       DD     LA NK WR+C  C+  +E + GC HI
Sbjct: 437 CKVPWHGNLSCTEYKKLHPEP----PADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHI 496

Query: 246 VCRCGSDFCYSCGAKW 250
            CRCG +FCY+CG  W
Sbjct: 497 TCRCGHEFCYNCGGGW 502

[Close]

BLAST of CsGy3G026530 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 145.6 bits (366), Expect = 5.8e-35
Identity = 76/253 (30.04%), Postives = 119/253 (47.04%), Query Frame = 0


Query: 6   SSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAKSHSQ 65
           +S+  +L    D+N+A  L +  ++ +              +    L CSIC+D      
Sbjct: 147 ASTGTVLVATRDDNFALRLAIDALVKA--------------TQEKPLTCSICSDKTDAEH 206

Query: 66  MFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWAD 125
           M  N  C H  C +C+   +  KL   +   C E  C + L  E C   +  +++E W  
Sbjct: 207 MLLNDKCLHRHCFSCVKQQVKVKLRSGIVPPCLEDGCKSELTLESCSMVLTPKLIEMWKR 266

Query: 126 ALFEAMILKWKRLNCPFKDCG-----AAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHG 185
            + E +I   +++ CP++ C        +  E  +  V A  C  C  LFC +C+V WH 
Sbjct: 267 KMEEDLIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRA--CIKCSELFCIDCKVPWHS 326

Query: 186 EMECGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSD 245
           ++ C +++R+  E  V    +D M   LA ++ WR+C  CK  +E T GC HI CRCG +
Sbjct: 327 DLSCADYKRIHSERLV----NDMMLKVLANDQMWRQCSECKHMIELTEGCNHITCRCGYE 379

Query: 246 FCYSCGAKWGGGH 254
           FCY CG KW   H
Sbjct: 387 FCYRCGHKWTKYH 379

[Close]

BLAST of CsGy3G026530 vs. TAIR 10
Match: AT3G45540.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 137.9 bits (346), Expect = 1.2e-32
Identity = 74/240 (30.83%), Postives = 120/240 (50.00%), Query Frame = 0


Query: 19  NYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAK-SHSQMFTNRVCTHTFC 78
           +YA +L  + ++  S +S  +NP P    +T +  C IC D   + +QMF    C H FC
Sbjct: 68  SYAYKLARETIV--SEISISVNP-PRQPKATRKTTCKICLDDDINENQMFCVGKCRHRFC 127

Query: 79  TACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERWADALFEAMILKWKR 138
           + C+  HI  +L     ++CP   C T L+   C + +  ++ E W   + E +I    R
Sbjct: 128 SDCMRRHIEVRLLEGSVMRCPHYRCKTTLKFGGCINLLTPKIREMWQQRIKEDLIPVTGR 187

Query: 139 LNCPFKDCGAAIIDEGGEEGVTAVE------CGSCWRLFCAECRVGWHGEMECGEFQRLR 198
           + CP   C A + +   E  ++  E      C  C ++FC +C+V WH  + C ++++L 
Sbjct: 188 IYCPNSRCSALMSET--ELSISTKEDEVRRCCFKCGQIFCIKCKVSWHSNLSCNDYKKLN 247

Query: 199 KEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCYSCGAKWGG 252
                   ++D     LA  K+WR+C  C+  +E + GCV + CRCG  FCY CG + GG
Sbjct: 248 PYP----TENDGKIKALANQKRWRQCGKCQHMIELSKGCVQVKCRCGHKFCYRCGVQAGG 298

The following BLAST results are available for this feature:

BLAST of CsGy3G026530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4IIY1	1.1e-17	27.50	Probable E3 ubiquitin-protein ligase RNF144A OS=Xenopus tropicalis OX=8364 GN=rn...	[more]
Q7Z419	5.3e-17	28.97	E3 ubiquitin-protein ligase RNF144B OS=Homo sapiens OX=9606 GN=RNF144B PE=1 SV=1	[more]
A5PK27	6.9e-17	29.49	E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1	[more]
Q8BKD6	1.2e-16	28.50	E3 ubiquitin-protein ligase RNF144B OS=Mus musculus OX=10090 GN=Rnf144b PE=2 SV=...	[more]
Q6T486	2.0e-16	26.19	Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G...	[more]

BLAST of CsGy3G026530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144476.2	4.49e-200	99.62	probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypot...	[more]
XP_008460341.1	8.49e-180	90.26	PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1...	[more]
XP_038889081.1	6.29e-132	68.94	probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida]	[more]
XP_022956145.1	1.72e-122	66.41	probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata]	[more]
XP_022980686.1	7.00e-117	63.92	probable E3 ubiquitin-protein ligase ARI5 [Cucurbita maxima]	[more]

BLAST of CsGy3G026530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L952	2.17e-200	99.62	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1	[more]
A0A5A7UED3	4.11e-180	90.26	E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3CDI0	4.11e-180	90.26	E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194...	[more]
A0A6J1GX07	8.32e-123	66.41	probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1IU95	3.39e-117	63.92	probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of CsGy3G026530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14250.1	3.6e-61	42.11	RING/U-box superfamily protein	[more]
AT3G53690.1	3.7e-50	43.28	RING/U-box superfamily protein	[more]
AT5G60250.1	5.8e-35	31.64	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G37560.1	5.8e-35	30.04	RING/U-box superfamily protein	[more]
AT3G45540.1	1.2e-32	30.83	RING/U-box protein with C6HC-type zinc finger	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 121..184 e-value: 1.3E-13 score: 61.2 coord: 199..255 e-value: 0.0084 score: 24.9
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 125..184 e-value: 4.9E-10 score: 39.5 coord: 207..255 e-value: 1.1E-8 score: 35.2
None	No IPR available	GENE3D	2.20.25.20		coord: 138..185 e-value: 3.7E-6 score: 28.9
None	No IPR available	GENE3D	1.20.120.1750		coord: 186..266 e-value: 1.5E-15 score: 59.7
None	No IPR available	PANTHER	PTHR11685:SF327	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 11..247
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 49..130
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 203..256
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 125..189
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 47..133 e-value: 1.3E-18 score: 68.7
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 11..247
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 72..81
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 50..264 score: 28.006748
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 54..98 score: 9.833738

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G026530.2	CsGy3G026530.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity