CsGy3G026030 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G026030
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description4-coumarate-CoA ligase
LocationGy14Chr3: 26803149 .. 26807553 (+)
RNA-Seq ExpressionCsGy3G026030
SyntenyCsGy3G026030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAATTGTGGACCAACATTATGAGTTTTATTGGTTTGATCACTGAATATTTAGTTCACACGATGTAATGTTTTGATATTTATTTGCTTGTTCTTTTCTTTCAGTTTATGAAAAATGGCACGGCTCATAAACAATCAGTTCAGTTTTGAAACGCCAGAAAATGAGGCTGTTATCACTAAAAAAATTCCTCGCTCTGAACGCACCAACAAGTATCCTAATTGGTATTCACCAGATACAGGAATTTGTCGTAGCGTCCATGGCTACAGAAAACTTCCCTCTGATCCATTTCTTGACGTTGTCTCCTTCATCTTCTCCTTCCAGTTCCAACACAATGGACATTCAGCTCTCATTGATTCCTCAACTGGAAATTCAATATCTTACAAAGAATTGTTTCCTATGGTAAAATCCATGGCTTCTGGCCTCCATAATTTAGGTATATCACAAGGGGATGTAGTTTTGCTTATGTTACCAAATTCAATTTTCTTTCCCATTATTATTTTGGGTGCCCTGTATTTGGGAGCTGTTGTTACAACCATGTTTCCTCAAAGCAGCTCCTCTGAAATCAAGAAACGAATTACTGATTGCAATGTGCGTTTGGCATTTGCAATAACTCAGAAAATCAAGAATTTTGAAGCATTGGGAATTAAGACAATTGGTGTACCAGAAAATACGAACTTCAACTTGATGAGGTCTATGGGATTTTCTTCTTTTTATGAACTTATTTCAGGTGGTTCTGATCTGATTAAAAAACCAGTGATCAGACAAGAAGATACAGCTGCTATATTGTTTTCTTCGGGTACTACTGGAGTGAGCAAAGGGGTTATGTTATCACATAGAAATTTTATATCTACCATCGAGCTTTTCGTTCGGTTTGAAGCTTCACAGTATGAATATTTGCCTACAAAGAATGTGTATTTGGCTGCTGTACCAATGTTTCATATCTATGGTCTCTCCATTTTTGTGATGGGATTAATGTCATTGGGGTCTAGTGTTGTTGTAATGAGCAAATTTGATGTCAAAGATGTGGTCAAGGCCATTGATAGATTTAAGGTCACACATTTTCCTGTTGTTCCACCTATATTGACAGCTTTGGCTAGGACTGCAGAGAAAATTGGTGTGCATAGATTTCGAAGTTTGAAACAAGTTTCTTGTGGGGCTGCTGCTTCAAGCAAGAAGACAATAGATAATTTTGTACACGCTCTCCCTCATGTTGACTTCATTCAGGTATTGATTAAACCAATTCTGTTTATGATGGATTGAGATAACTTAGAACAGGCTTAATTTCAAAAACAAAAGGGTGAAAAAATTGGTTTAATTTTCAGAAATGAAAAACAAAATGGTTACCTTACTTTTGTTTGTGAAGCATCCAAAATAATGTCTGAGTCCTGTTCTGTATCTTGTTTGGAAATAGAGGAACTTTGACTCATAATGTTGATGTGCTATGTTTGTTAGTTTGAACAAAGCTTGGTTTGGTGTATTTGTGGTTAGGGATATGGCATGACAGAGTCCACAGCTGTAGGAACCCGTGGTTTCAATACCAAAAATGCTAGAAATTATTTGTCAGTGGGACTTTTAGCTCCAAACACAGAAGCTAAGGTCGTCGATTGGGTTACTGGATCTTCCATGCCTCCTGGTAAAACCGGCGAACTTTTATTACGTGGACCTGGTTCAATGAAAGGTTTGATTGATTCTTTTTAACACAATCTGCGCACTGCCCGAAGATCTTGTTAACTACCATTTACTTTAAAGTTTATACTAATAGGTTTCAATATATCTTTTGTTTATATTACTACTAAATCTAAAGGTCGAAGTTGATGAGTTATAACTCACAACATATTTAATACTTTGTTTCTATTCTTAACCTATCTATTTGTTGGCTGTAGTGAATTTCACATTTCTCTTCCGTATTTTTCTGTCACTTTCATCAGTCTAGAGTTCATAGCTGTCTCCATTACAGTGTATCTACCCTCACCATTTAAGAAGCACGAGTATGGATATGGCGTAACATAAACATAGTGACATGCCATATTTTAGACATCTAGGGCACTACACGGTGATGCCACATTTATTAGAATACGCATCTTTCAAAATATATATCATTTTTATACAAAAGGAAAATTTAAAGCAAATGGGTTGAATGTTTATGAATGTATGTACTCAAAAAACACTTAGCTTGATGTATTCATTCAAAAGTTATTATTAATTATTGTCATGCAGGTGTCTTTTCAATATTCTCAACAAGTTAACACATTTGTTGCATTAATAAGTGTTTGATACATGTCTAACAAGTATATATATAGTAGTGTGTTGAACACAGAAACACAATGTCAAACTGAAGTGTTTGTGCTTCTTTGTAGTCACCACAATCACAAAGATAAACTAACTACAATTGGAATGCTTATTCTGAAACGTTGTTTTGTTTTGTGTTTACAGGGTACTTGAATAATCCAGAGGCAACAACGTTCACAATGGACCAAGAAAATTGGCTACATACGGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTGTTGATCGCTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGGTACAATATATCCAAATTTTCATGTCTGTTGCTTTGTCAAAAATACTACTACAGCTTATGAGTAATCACACTCGTGCAAGTTTAGAGTGATTTACATTTGTTTTTAGCCAAGGACAATTGCAGAGATGACAAAATTTAGACTTATCACTAGGAGTGATCTATCACTGCTAAATCATATCACTTGAAGAAGCCAATCAACAATAAAATCTATCGTCAATAGATTTTGTTATATTTGCAATTCTGTAAAAATGTTACTATATACTTAATTATTATTTTAAAAAATGCTATCTAATGTGCAATTATCCTAGCTTTATATAACTGACGTTAACTTCTCAGCCAATAAAAATAAAATATGGTTACCAAACCAAACAGAGCTTTAAATACATACAAGTTTATCCTTTCGAGGAGTTAAAGCATTGATTTTTCACTCTGATCTTGCTTGTGATATGTCAATGTTTTGAATGAAGTTCGTGATGGATAAAGCTAAGGTAGTCATGTAATTTGCTTAATCTGTTATATTTTTGTAGATTGCACCTACAGATTTAGAAGCTGTAGTGATCACACATCCTGAAGTTCTTGATACTGCAGTAGCAGCGTAAGTATCATTCTAATCTTTTAGTAAGCCACTGTGTCATTTCAGTCAAAACTATTACACTTTAGAATCTAGGGGGACATTAAATTATTCTAAAAGTGTGCATATATAACTTTTAAGTGAATATAAAAACATCCCTAGATTTATGAAGAAGATTGTAAGAAAATATCACAAAGAAAAAATTTAGAAATATCTCAAGCTCTCTCTTTAATCTCGAAGGAATTCTAAAACTCTTTTAGGCAAGGACAATGTCATAAAAATATCTAGAATAGTTAAGATAAAGAGAAAGAAAGTGTTGAAAAATCCTATGGCTAAGCTCAAAGATCTCACACATATAAACAGAACGTTTTATTCTCATTTGTAAAGCAAATGAAGAAAATAAAAGAAGAAAGAAATCGAAGCTACGAAGTTAAAAAGTTAGACAACATAGATGAAGAAAATTGAGGGGCCATCTTTATAGATTCTTTATAAAAGTTTCTTTGTTCCTCGTGATTCTCTCTCTCTCTCTCTCTCTCTTTTTTCTTTTGCCAATTTCTTCAACACAAGTGTCAAACAAGAAAACACAAACAAATTGGGGGACAAAGTGACGTGAATCCTTGTAAATTTTAATCATCATCAGCCAAGCCTATTTGGAGGCTGTTTTAATTTAATTTTCAGATAATTTCAGATCATATGTTAGAGGCATTTTTCATGTGTTTTTTCAGGGCTAAAGATGAAGAATGTGGTGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGTGCACTCACACAAAAAGATGTTGTTGATTATGTTGCTCAACAGGTTTCCCATATTTTTCGAATCAATCTGCCCATGCATTTTTTAAACATTAAAAACGTTTAAATTATATACATATATTCTTTTGTCTCTGCAGGTTGCACCATATAAAAAAGTTAGAAAAGTGATTTTTACGGAATCAATACCAAAGTCTGCTGCTGGGAAAGTACTTCGAAGGGAACTACAGAAACACTTCCCAGCTTCTAAATTATGATGACCTCTCCAGCCATTGTTTTTGTTCCTTTTTTTTTCCTTATAACTAATTGGCTCTACCACCCCTAATATTTGTAATAACGGTATTTTGTGACAATTACTTTATTGCAATTCAATCCCTATCCTTGAAGAGTTCTTGTGTTAAAAAAGTCTAAGTTAAATCTCCGAAATCTAATAACTTCAACATCTAGTAACTTCAACTAAGATTTTGTTTTGTGT

mRNA sequence

GGAAAATTGTGGACCAACATTATGAGTTTTATTGGTTTGATCACTGAATATTTAGTTCACACGATGTAATGTTTTGATATTTATTTGCTTGTTCTTTTCTTTCAGTTTATGAAAAATGGCACGGCTCATAAACAATCAGTTCAGTTTTGAAACGCCAGAAAATGAGGCTGTTATCACTAAAAAAATTCCTCGCTCTGAACGCACCAACAAGTATCCTAATTGGTATTCACCAGATACAGGAATTTGTCGTAGCGTCCATGGCTACAGAAAACTTCCCTCTGATCCATTTCTTGACGTTGTCTCCTTCATCTTCTCCTTCCAGTTCCAACACAATGGACATTCAGCTCTCATTGATTCCTCAACTGGAAATTCAATATCTTACAAAGAATTGTTTCCTATGGTAAAATCCATGGCTTCTGGCCTCCATAATTTAGGTATATCACAAGGGGATGTAGTTTTGCTTATGTTACCAAATTCAATTTTCTTTCCCATTATTATTTTGGGTGCCCTGTATTTGGGAGCTGTTGTTACAACCATGTTTCCTCAAAGCAGCTCCTCTGAAATCAAGAAACGAATTACTGATTGCAATGTGCGTTTGGCATTTGCAATAACTCAGAAAATCAAGAATTTTGAAGCATTGGGAATTAAGACAATTGGTGTACCAGAAAATACGAACTTCAACTTGATGAGGTCTATGGGATTTTCTTCTTTTTATGAACTTATTTCAGGTGGTTCTGATCTGATTAAAAAACCAGTGATCAGACAAGAAGATACAGCTGCTATATTGTTTTCTTCGGGTACTACTGGAGTGAGCAAAGGGGTTATGTTATCACATAGAAATTTTATATCTACCATCGAGCTTTTCGTTCGGTTTGAAGCTTCACAGTATGAATATTTGCCTACAAAGAATGTGTATTTGGCTGCTGTACCAATGTTTCATATCTATGGTCTCTCCATTTTTGTGATGGGATTAATGTCATTGGGGTCTAGTGTTGTTGTAATGAGCAAATTTGATGTCAAAGATGTGGTCAAGGCCATTGATAGATTTAAGGTCACACATTTTCCTGTTGTTCCACCTATATTGACAGCTTTGGCTAGGACTGCAGAGAAAATTGGTGTGCATAGATTTCGAAGTTTGAAACAAGTTTCTTGTGGGGCTGCTGCTTCAAGCAAGAAGACAATAGATAATTTTGTACACGCTCTCCCTCATGTTGACTTCATTCAGGGATATGGCATGACAGAGTCCACAGCTGTAGGAACCCGTGGTTTCAATACCAAAAATGCTAGAAATTATTTGTCAGTGGGACTTTTAGCTCCAAACACAGAAGCTAAGGTCGTCGATTGGGTTACTGGATCTTCCATGCCTCCTGGTAAAACCGGCGAACTTTTATTACGTGGACCTGGTTCAATGAAAGGGTACTTGAATAATCCAGAGGCAACAACGTTCACAATGGACCAAGAAAATTGGCTACATACGGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTGTTGATCGCTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGATTGCACCTACAGATTTAGAAGCTGTAGTGATCACACATCCTGAAGTTCTTGATACTGCAGTAGCAGCGGCTAAAGATGAAGAATGTGGTGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGTGCACTCACACAAAAAGATGTTGTTGATTATGTTGCTCAACAGGTTGCACCATATAAAAAAGTTAGAAAAGTGATTTTTACGGAATCAATACCAAAGTCTGCTGCTGGGAAAGTACTTCGAAGGGAACTACAGAAACACTTCCCAGCTTCTAAATTATGATGACCTCTCCAGCCATTGTTTTTGTTCCTTTTTTTTTCCTTATAACTAATTGGCTCTACCACCCCTAATATTTGTAATAACGGTATTTTGTGACAATTACTTTATTGCAATTCAATCCCTATCCTTGAAGAGTTCTTGTGTTAAAAAAGTCTAAGTTAAATCTCCGAAATCTAATAACTTCAACATCTAGTAACTTCAACTAAGATTTTGTTTTGTGT

Coding sequence (CDS)

ATGGCACGGCTCATAAACAATCAGTTCAGTTTTGAAACGCCAGAAAATGAGGCTGTTATCACTAAAAAAATTCCTCGCTCTGAACGCACCAACAAGTATCCTAATTGGTATTCACCAGATACAGGAATTTGTCGTAGCGTCCATGGCTACAGAAAACTTCCCTCTGATCCATTTCTTGACGTTGTCTCCTTCATCTTCTCCTTCCAGTTCCAACACAATGGACATTCAGCTCTCATTGATTCCTCAACTGGAAATTCAATATCTTACAAAGAATTGTTTCCTATGGTAAAATCCATGGCTTCTGGCCTCCATAATTTAGGTATATCACAAGGGGATGTAGTTTTGCTTATGTTACCAAATTCAATTTTCTTTCCCATTATTATTTTGGGTGCCCTGTATTTGGGAGCTGTTGTTACAACCATGTTTCCTCAAAGCAGCTCCTCTGAAATCAAGAAACGAATTACTGATTGCAATGTGCGTTTGGCATTTGCAATAACTCAGAAAATCAAGAATTTTGAAGCATTGGGAATTAAGACAATTGGTGTACCAGAAAATACGAACTTCAACTTGATGAGGTCTATGGGATTTTCTTCTTTTTATGAACTTATTTCAGGTGGTTCTGATCTGATTAAAAAACCAGTGATCAGACAAGAAGATACAGCTGCTATATTGTTTTCTTCGGGTACTACTGGAGTGAGCAAAGGGGTTATGTTATCACATAGAAATTTTATATCTACCATCGAGCTTTTCGTTCGGTTTGAAGCTTCACAGTATGAATATTTGCCTACAAAGAATGTGTATTTGGCTGCTGTACCAATGTTTCATATCTATGGTCTCTCCATTTTTGTGATGGGATTAATGTCATTGGGGTCTAGTGTTGTTGTAATGAGCAAATTTGATGTCAAAGATGTGGTCAAGGCCATTGATAGATTTAAGGTCACACATTTTCCTGTTGTTCCACCTATATTGACAGCTTTGGCTAGGACTGCAGAGAAAATTGGTGTGCATAGATTTCGAAGTTTGAAACAAGTTTCTTGTGGGGCTGCTGCTTCAAGCAAGAAGACAATAGATAATTTTGTACACGCTCTCCCTCATGTTGACTTCATTCAGGGATATGGCATGACAGAGTCCACAGCTGTAGGAACCCGTGGTTTCAATACCAAAAATGCTAGAAATTATTTGTCAGTGGGACTTTTAGCTCCAAACACAGAAGCTAAGGTCGTCGATTGGGTTACTGGATCTTCCATGCCTCCTGGTAAAACCGGCGAACTTTTATTACGTGGACCTGGTTCAATGAAAGGGTACTTGAATAATCCAGAGGCAACAACGTTCACAATGGACCAAGAAAATTGGCTACATACGGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTGTTGATCGCTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGATTGCACCTACAGATTTAGAAGCTGTAGTGATCACACATCCTGAAGTTCTTGATACTGCAGTAGCAGCGGCTAAAGATGAAGAATGTGGTGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGTGCACTCACACAAAAAGATGTTGTTGATTATGTTGCTCAACAGGTTGCACCATATAAAAAAGTTAGAAAAGTGATTTTTACGGAATCAATACCAAAGTCTGCTGCTGGGAAAGTACTTCGAAGGGAACTACAGAAACACTTCCCAGCTTCTAAATTATGA

Protein sequence

MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL*
Homology
BLAST of CsGy3G026030 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 636.3 bits (1640), Expect = 3.3e-181
Identity = 318/548 (58.03%), Postives = 423/548 (77.19%), Query Frame = 0

Query: 25  PRSERTNKYPN-WYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSST 84
           P+++ +++ P  W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDS T
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLT 71

Query: 85  GNSISYKELFPMVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMF 144
           G SIS+ EL  MV+SMA+G+ H LG+ QGDVV L+LPNS++FP+I L  + LGA+VTTM 
Sbjct: 72  GFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 131

Query: 145 PQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYEL 204
           P SS  EIKK++++C+V LAF  T+ ++   +LG+  I V E+ +F+ +R +    FY +
Sbjct: 132 PSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSI 191

Query: 205 ISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 264
           +      + KP+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  
Sbjct: 192 MKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPG 251

Query: 265 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 324
           + NVYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+
Sbjct: 252 SSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 311

Query: 325 LTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGT 384
           L AL + A+ +    F+SLKQVS GAA  S+K I++F+  LPHVD IQGYGMTESTAVGT
Sbjct: 312 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT 371

Query: 385 RGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 444
           RGFN++    Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT
Sbjct: 372 RGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKAT 431

Query: 445 TFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTA 504
             ++ +++WL TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D A
Sbjct: 432 QMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAA 491

Query: 505 VAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 564
           V AA +EECGEIPVAFVV++  + L+++DV+ YVA QVAPY+KVRKV+   SIPKS  GK
Sbjct: 492 VTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGK 551

Query: 565 VLRRELQK 571
           +LR+EL++
Sbjct: 552 ILRKELKR 556

BLAST of CsGy3G026030 vs. ExPASy Swiss-Prot
Match: Q6YYZ2 (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.9e-144
Identity = 286/550 (52.00%), Postives = 366/550 (66.55%), Query Frame = 0

Query: 34  PNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFS-FQFQHNGHSALIDSSTGNSISYKEL 93
           P +YS  TGI  S+H    LP+DP L +V+ +F+       G   L+D++T +++S  +L
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 94  FPMVKSMASGLHNL-GISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIK 153
             +V S+A+GL    G+ +G VVLL+LPNS+ FP+  L  L  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 154 KRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMG------FSSFYELISG 213
            +       L  A          L    + VPE  + +     G      F++F  ++ G
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 214 G---SDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 273
           G         PV+ Q+D  AIL+SSGT+G SKGV+L+HRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 274 TK-NVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 333
            + NVY+AA+PM H+YGLS+F +GL+S+G++VVVM +FD  D V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 334 ILTALARTAEKIGV--HRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTA 393
           I+ A+ R A   GV   +  SL QVSCGAA  +   I  F+ A PHVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 394 VGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNP 453
           VGTRGFNT   + Y SVGLLAPN  AK+V   + S +PPG +GEL L GPG MKGYL++ 
Sbjct: 385 VGTRGFNTSKHKKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSDD 444

Query: 454 EATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVL 513
           +      D   WL TGDI YFD DGYLY+V RLK+ IKYKGFQIAP DLE V+I HPE+L
Sbjct: 445 DDACTRKD--GWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEIL 504

Query: 514 DTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSA 570
           D AV +A+DEE GEIPVAFVV++ GS L+ K V++YVA+QVAPYK+VRKV+F E+IPKS 
Sbjct: 505 DVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKSP 564

BLAST of CsGy3G026030 vs. ExPASy Swiss-Prot
Match: Q336M7 (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 464.5 bits (1194), Expect = 1.7e-129
Identity = 262/515 (50.87%), Postives = 341/515 (66.21%), Query Frame = 0

Query: 35  NWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFP 94
           ++YS  TG+  S+H    LPSDP L +V  +FS    H+ HS L+D+ T  ++S  +   
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHH-HSLLVDAPTAATLSCADFRR 73

Query: 95  MVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKR 154
           +V S+A+GL   L I++G +VLL+LPNS+ FP+  L  L  GAV TTM P S+ +EI  R
Sbjct: 74  LVSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAAR 133

Query: 155 ITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDL-IKK 214
           + D    L  A T        L    + VP+              F+ L+    +  ++ 
Sbjct: 134 LRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEM 193

Query: 215 PV-IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAV 274
            V + Q+D AA+L+SSGT+G SKGV+++HRN I+ +ELFVRFEASQY      NVYLAA+
Sbjct: 194 GVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAAL 253

Query: 275 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAE 334
           PMFH+YGLS+F +GL+SLG +VVVM +F+V D VKAI ++KVTH P+VPPI++AL R   
Sbjct: 254 PMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANP 313

Query: 335 KIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNAR 394
            +      SL QVS GAA  +   I +F+HA PHVDFIQGYGMTESTAVGTRGFNT   +
Sbjct: 314 PL---ELDSLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKHK 373

Query: 395 NYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMK------GYLNNPEATTFT 454
            Y SVGLLAPN  AK+V   +GS +PPG  GEL L GP  MK      GYLN+ +   FT
Sbjct: 374 KYASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDD-DAFT 433

Query: 455 MDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAA 514
             ++ WL TGDI YFD DGYL++V RLK+ IKYKGFQIAP DLEAV+I HPE++D AV +
Sbjct: 434 R-KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVTS 493

Query: 515 AKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 541
            +DEE GEIPVAFVV+K GS L+   V++YVA+QV
Sbjct: 494 DEDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV 522

BLAST of CsGy3G026030 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.9e-122
Identity = 227/527 (43.07%), Postives = 350/527 (66.41%), Query Frame = 0

Query: 52  KLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQG 111
           +LP +  +DV +FI S    H+G  A ID++TG  +++ +L+  V S+A+ L  +GI +G
Sbjct: 30  QLPQNHSVDVTTFISS--RAHHGKIAFIDAATGRHLTFPQLWRAVDSVATCLSAMGIRKG 89

Query: 112 DVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKN 171
           DV+LL+ PNSI+FP++ L  + LGA++TT  P ++  EI K+ITD    LAF I Q +  
Sbjct: 90  DVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSKPVLAFTIPQLVSK 149

Query: 172 FEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPV-------IRQEDTAAIL 231
                +  + + +    +L +++   S     S G  + K+P        + QEDTA +L
Sbjct: 150 IAGSNLPIVIIDDEVKSSLEKTLNIVS-----SLGEMMRKEPSPNRIGYRVNQEDTATLL 209

Query: 232 FSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVM 291
           +SSGTTG SKGV+ SH+N I+ ++  +    S++     ++ ++  VPMFHIYGL+ F M
Sbjct: 210 YSSGTTGASKGVVSSHKNLIAMVQTIL----SRFGTEDGEHTFICTVPMFHIYGLAAFAM 269

Query: 292 GLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAEKI-GVHRFRSLKQ 351
           GL+S GS++V++SKF++ +++ AI++++ T+ P+VPPIL AL + A  I   +   SL+ 
Sbjct: 270 GLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIRAKYDLSSLQS 329

Query: 352 VSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNT 411
           V  G A  SK+ I+ FV   P V  +QGYG+TEST +G      + +R Y + G+L+P+ 
Sbjct: 330 VLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRYGTAGMLSPSM 389

Query: 412 EAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRD 471
           EAK+V+  TG ++   +TGEL LRGP  MKGY +N EAT+ T+D E WL TGD+ Y D D
Sbjct: 390 EAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLRTGDLCYIDED 449

Query: 472 GYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKP 531
           G+++VVDRLKE+IKYKG+Q+AP +LEA++++HPE+ D AV    D+E G+ P+A+VV+K 
Sbjct: 450 GFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEAGQFPMAYVVRKG 509

Query: 532 GSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRRELQK 571
           GS L++  V+D++A+ VAPYK++RKV F  SIPK+ +GK+LR++L K
Sbjct: 510 GSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIK 545

BLAST of CsGy3G026030 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 434.9 bits (1117), Expect = 1.4e-120
Identity = 236/549 (42.99%), Postives = 351/549 (63.93%), Query Frame = 0

Query: 26  RSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGN 85
           RS   N    +YS  T I         LP +P LDV +FI S    H G  A ID+STG 
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISS--QAHRGRIAFIDASTGQ 66

Query: 86  SISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQS 145
           ++++ EL+  V+S+A  L  +GI +G VVLL+ PNSI FP++ L  + LGA++TT  P +
Sbjct: 67  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 126

Query: 146 SSSEIKKRITDCNVRLAFAITQKIKNFEALGIK--TIGVPENTNFNLMRSMGFSSFYELI 205
           +S+EI K+I D N  LAF  +Q +    A   K   + + E    ++          +  
Sbjct: 127 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 186

Query: 206 SGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQYEYLP 265
             G+ +  K  + Q+DTA +L+SSGTTG+SKGV+ SHRN I+ ++  V RF +   E   
Sbjct: 187 PSGNRV--KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGE--- 246

Query: 266 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 325
               ++  VPMFHIYGL+ F  GL++ GS+++V+SKF++ +++ AI +++ T  P+VPPI
Sbjct: 247 --QRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 306

Query: 326 LTALARTAEKI-GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVG 385
           L A+   A++I   +   S+  V CG A  SK+  + F    P V  +QGYG+TEST +G
Sbjct: 307 LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIG 366

Query: 386 TRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEA 445
                 + +R Y + G L+ + E ++VD VTG  + P +TGEL L+GP  MKGY +N EA
Sbjct: 367 ASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEA 426

Query: 446 TTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDT 505
           T+ T+D E WL TGD+ Y D DG+++VVDRLKE+IKYKG+Q+AP +LEA+++THPE+ D 
Sbjct: 427 TSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDA 486

Query: 506 AVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAG 565
           AV    D+E G+ P+A+VV+K GS+L++K ++++VA+QVAPYK++RKV F  SIPK+ +G
Sbjct: 487 AVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSG 538

Query: 566 KVLRRELQK 571
           K+LR++L K
Sbjct: 547 KILRKDLIK 538

BLAST of CsGy3G026030 vs. NCBI nr
Match: XP_004144410.1 (4-coumarate--CoA ligase-like 6 [Cucumis sativus] >KGN58405.1 hypothetical protein Csa_001031 [Cucumis sativus])

HSP 1 Score: 1130 bits (2923), Expect = 0.0
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
           VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
           HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577
           APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

BLAST of CsGy3G026030 vs. NCBI nr
Match: TYK21080.1 (4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 546/578 (94.46%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577
           VAPYKKVRKVIFTESIPKSAAGKVLRRELQKH PASKL
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578

BLAST of CsGy3G026030 vs. NCBI nr
Match: XP_008460452.1 (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 512/552 (92.75%), Postives = 527/552 (95.47%), Query Frame = 0

Query: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIF 551
           V+   ++   IF
Sbjct: 541 VSHIFQICPCIF 552

BLAST of CsGy3G026030 vs. NCBI nr
Match: KAA0068160.1 (4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa])

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 509/540 (94.26%), Postives = 521/540 (96.48%), Query Frame = 0

Query: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 539
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of CsGy3G026030 vs. NCBI nr
Match: XP_038888724.1 (4-coumarate--CoA ligase-like 6 [Benincasa hispida])

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 499/574 (86.93%), Postives = 542/574 (94.43%), Query Frame = 0

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MA L+N+ FS++TPENE VITKKIPRSE+TNKYPNWYSPDTGICRSVH  R +PSDPFLD
Sbjct: 1   MAPLLNDHFSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           VVSFIFSF+  HNG+SA+IDSSTG+SISYKE+F MVKSMASGL   GISQGDVVLL+LPN
Sbjct: 61  VVSFIFSFK--HNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           SIF+PII+LG LYLGAVVTTMFPQSSSSEIKKRI DCNVRLAF ITQKIKNFE+LGI+ I
Sbjct: 121 SIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQAI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPE TNF+LM+ MGFS F+ELIS GSDL ++PVIRQEDTAA+LFSSGTTGVSKGVML+H
Sbjct: 181 GVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLTH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFISTIELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGL+SLGSSVV+MSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKFD 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
           VK+VVKAIDRFKVTHFPVVPPI+TA+ARTAEKIGVHRFRSLKQVSCGAA+ +KKTID+FV
Sbjct: 301 VKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
            A PHVDFIQGYGMTESTA+GTRGFNTKNARNY SVGLLAPNTEAKVVDWV+GS MPPGK
Sbjct: 361 KAFPHVDFIQGYGMTESTAIGTRGFNTKNARNYTSVGLLAPNTEAKVVDWVSGSFMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEAT  T+D+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           FQIAPTDLEAVV+THPEVLDTAV AAKDEECGEIPVAFVVKKPGS L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574
           APYKKVRKV+FTESIPKSAAGKVLRREL KH P+
Sbjct: 541 APYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 572

BLAST of CsGy3G026030 vs. ExPASy TrEMBL
Match: A0A0A0L8X1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1130 bits (2923), Expect = 0.0
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD
Sbjct: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN
Sbjct: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI
Sbjct: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH
Sbjct: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD
Sbjct: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
           VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV
Sbjct: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
           HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK
Sbjct: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
           TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV
Sbjct: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577
           APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL
Sbjct: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

BLAST of CsGy3G026030 vs. ExPASy TrEMBL
Match: A0A5D3DC80 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00450 PE=4 SV=1)

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 546/578 (94.46%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577
           VAPYKKVRKVIFTESIPKSAAGKVLRRELQKH PASKL
Sbjct: 541 VAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578

BLAST of CsGy3G026030 vs. ExPASy TrEMBL
Match: A0A1S3CCH7 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo OX=3656 GN=LOC103499264 PE=4 SV=1)

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 512/552 (92.75%), Postives = 527/552 (95.47%), Query Frame = 0

Query: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 540
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

Query: 541 VAPYKKVRKVIF 551
           V+   ++   IF
Sbjct: 541 VSHIFQICPCIF 552

BLAST of CsGy3G026030 vs. ExPASy TrEMBL
Match: A0A5A7VLT7 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G001280 PE=4 SV=1)

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 509/540 (94.26%), Postives = 521/540 (96.48%), Query Frame = 0

Query: 1   MARLINN-QFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60
           MARL++N QFSFETPENEA ITKKIP SERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENTNF+LMR MGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV RFRSLKQVSCGAAASSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420
           V AL HVDFIQGYGMTESTA+GTRGFN K  RNYLSVGLLAPNTEAKVVDWVTGSSMPPG
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNKKTRNYLSVGLLAPNTEAKVVDWVTGSSMPPG 420

Query: 421 KTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480
           KTGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK
Sbjct: 421 KTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYK 480

Query: 481 GFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQ 539
           GFQIAPTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQQ
Sbjct: 481 GFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQ 540

BLAST of CsGy3G026030 vs. ExPASy TrEMBL
Match: A0A6J1HGK9 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 472/574 (82.23%), Postives = 522/574 (90.94%), Query Frame = 0

Query: 1   MARLINNQFSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLD 60
           MA L+N+QFSF+TPE++ V TKKIP     NKYP WYSPDTGICRSVH  R LP+DPFLD
Sbjct: 1   MAPLLNDQFSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFLD 60

Query: 61  VVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPN 120
           V S+I SFQ  HNG SALIDSSTG+SISY+EL+ MV SMASGL  LG+SQGDVVLL+LPN
Sbjct: 61  VASYILSFQ--HNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLPN 120

Query: 121 SIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTI 180
           SIF+PI++LG LYLGAV+TTMFPQSSS EIKKRI++CNVRLAFA  Q + NFEA G++ I
Sbjct: 121 SIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQAI 180

Query: 181 GVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSH 240
           GVPENTN +LMR MGFSSFYELISGG DL K+PVIRQEDTAAILFSSGTTGVSKGV+L+H
Sbjct: 181 GVPENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLTH 240

Query: 241 RNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFD 300
           RNFIST+ELFVRFEASQYEYL T+NVYLAA+PMFH+YGLSIFVMGL+SLGSS+VVMSKFD
Sbjct: 241 RNFISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKFD 300

Query: 301 VKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFV 360
           VK+VV AIDRFKVTHFPVVPPI+  +ARTA K G H+FRSLKQVSCGAA+ SKK I +FV
Sbjct: 301 VKEVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDFV 360

Query: 361 HALPHVDFIQGYGMTESTAVGTRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGK 420
            ALPHVDFIQGYGMTE+TAVGTRGFNTK A+NYLSVGLLAPN EAKVVDWV+GS MPPGK
Sbjct: 361 QALPHVDFIQGYGMTETTAVGTRGFNTKKAQNYLSVGLLAPNMEAKVVDWVSGSIMPPGK 420

Query: 421 TGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480
           TGELLLRGPG MKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG
Sbjct: 421 TGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKG 480

Query: 481 FQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQV 540
           FQIAPTDLE+VVITHPEVLDTAVAAA+DEECGEIPVAFVVKKPGS+L+QKDV+DYVAQQV
Sbjct: 481 FQIAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQQV 540

Query: 541 APYKKVRKVIFTESIPKSAAGKVLRRELQKHFPA 574
           APYKK+RKV+FTESIPKSAAGKVLR+EL KH P+
Sbjct: 541 APYKKIRKVVFTESIPKSAAGKVLRKELGKHLPS 567

BLAST of CsGy3G026030 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 636.3 bits (1640), Expect = 2.3e-182
Identity = 318/548 (58.03%), Postives = 423/548 (77.19%), Query Frame = 0

Query: 25  PRSERTNKYPN-WYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSST 84
           P+++ +++ P  W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDS T
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLT 71

Query: 85  GNSISYKELFPMVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMF 144
           G SIS+ EL  MV+SMA+G+ H LG+ QGDVV L+LPNS++FP+I L  + LGA+VTTM 
Sbjct: 72  GFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 131

Query: 145 PQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYEL 204
           P SS  EIKK++++C+V LAF  T+ ++   +LG+  I V E+ +F+ +R +    FY +
Sbjct: 132 PSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSI 191

Query: 205 ISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 264
           +      + KP+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  
Sbjct: 192 MKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPG 251

Query: 265 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 324
           + NVYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+
Sbjct: 252 SSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 311

Query: 325 LTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGT 384
           L AL + A+ +    F+SLKQVS GAA  S+K I++F+  LPHVD IQGYGMTESTAVGT
Sbjct: 312 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT 371

Query: 385 RGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 444
           RGFN++    Y SVGLLAPN +AKVVDW +GS +PPG  GEL ++GPG MKGYLNNP+AT
Sbjct: 372 RGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKAT 431

Query: 445 TFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTA 504
             ++ +++WL TGDI YFD DGYL++VDR+KE+IKYKGFQIAP DLEAV+++HP ++D A
Sbjct: 432 QMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAA 491

Query: 505 VAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 564
           V AA +EECGEIPVAFVV++  + L+++DV+ YVA QVAPY+KVRKV+   SIPKS  GK
Sbjct: 492 VTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTGK 551

Query: 565 VLRRELQK 571
           +LR+EL++
Sbjct: 552 ILRKELKR 556

BLAST of CsGy3G026030 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 434.9 bits (1117), Expect = 1.0e-121
Identity = 236/549 (42.99%), Postives = 351/549 (63.93%), Query Frame = 0

Query: 26  RSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGN 85
           RS   N    +YS  T I         LP +P LDV +FI S    H G  A ID+STG 
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISS--QAHRGRIAFIDASTGQ 66

Query: 86  SISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQS 145
           ++++ EL+  V+S+A  L  +GI +G VVLL+ PNSI FP++ L  + LGA++TT  P +
Sbjct: 67  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 126

Query: 146 SSSEIKKRITDCNVRLAFAITQKIKNFEALGIK--TIGVPENTNFNLMRSMGFSSFYELI 205
           +S+EI K+I D N  LAF  +Q +    A   K   + + E    ++          +  
Sbjct: 127 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 186

Query: 206 SGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQYEYLP 265
             G+ +  K  + Q+DTA +L+SSGTTG+SKGV+ SHRN I+ ++  V RF +   E   
Sbjct: 187 PSGNRV--KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGE--- 246

Query: 266 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 325
               ++  VPMFHIYGL+ F  GL++ GS+++V+SKF++ +++ AI +++ T  P+VPPI
Sbjct: 247 --QRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 306

Query: 326 LTALARTAEKI-GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVG 385
           L A+   A++I   +   S+  V CG A  SK+  + F    P V  +QGYG+TEST +G
Sbjct: 307 LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIG 366

Query: 386 TRGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEA 445
                 + +R Y + G L+ + E ++VD VTG  + P +TGEL L+GP  MKGY +N EA
Sbjct: 367 ASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEA 426

Query: 446 TTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDT 505
           T+ T+D E WL TGD+ Y D DG+++VVDRLKE+IKYKG+Q+AP +LEA+++THPE+ D 
Sbjct: 427 TSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDA 486

Query: 506 AVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAG 565
           AV    D+E G+ P+A+VV+K GS+L++K ++++VA+QVAPYK++RKV F  SIPK+ +G
Sbjct: 487 AVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSG 538

Query: 566 KVLRRELQK 571
           K+LR++L K
Sbjct: 547 KILRKDLIK 538

BLAST of CsGy3G026030 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 419.1 bits (1076), Expect = 5.8e-117
Identity = 227/535 (42.43%), Postives = 338/535 (63.18%), Query Frame = 0

Query: 42  GICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMAS 101
           GI RS+     LP DP   +VSF+F     +    A+ DS TG+S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 102 GLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRL 161
           G H LGI + DVVL+  PNS  FP+  L    +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 162 AFAITQ---KIKNFE----ALGIK-TIGVPENTNFNLMRSMGFSSFYELISGGSDLIKKP 221
             ++ Q   KIK F+     LG K T+ +P  +N  +   + F +  EL    S+     
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKI---LSFDNVMELSEPVSE-YPFV 189

Query: 222 VIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPM 281
            I+Q DTAA+L+SSGTTG SKGV L+H NFI+   +    +    EY     V+L  +PM
Sbjct: 190 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPM 249

Query: 282 FHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALARTAEKI 341
           FH++GL++     +  G+++V M++F+++ V+K I++F+VTH  VVPP+  AL++ +  +
Sbjct: 250 FHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQS-IV 309

Query: 342 GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFNTKNARNY 401
                 SLK +  GAA   K  ++     +P+V  +QGYGMTE+  + +        RN 
Sbjct: 310 KKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNS 369

Query: 402 LSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLH 461
            S G+LAP  EA++V   TG S PP + GE+ +RGP  MKGYLNNP+AT  T+D+++W+H
Sbjct: 370 GSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVH 429

Query: 462 TGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGE 521
           TGD+ YF+ DG LYVVDR+KE+IKYKGFQ+AP +LE ++++HP++LD  V    DEE GE
Sbjct: 430 TGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGE 489

Query: 522 IPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRREL 569
           +P+AFVV+ P S++T++D+  ++A+QVAPYK++R+V F   +PKSAAGK+LRREL
Sbjct: 490 VPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

BLAST of CsGy3G026030 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 402.9 bits (1034), Expect = 4.3e-112
Identity = 222/544 (40.81%), Postives = 331/544 (60.85%), Query Frame = 0

Query: 37  YSPDTGICRSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMV 96
           +   T I  S      LP + FLDV SFI S    H G +  +D+ TG  +S+ EL+  V
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIAS--QPHRGKTVFVDAVTGRRLSFPELWLGV 86

Query: 97  KSMASGLHNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITD 156
           + +A  L+ LG+ +G+VV+++ PNSI FPI+ L  + LGA++TT  P ++S EI K+I D
Sbjct: 87  ERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGD 146

Query: 157 CNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLIK----- 216
               LAF   + +    A     + V    +++ + S  +    +L+     +I+     
Sbjct: 147 SRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYH-VPSQSYGDRVKLVGRLETMIETEPSE 206

Query: 217 ---KPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELF-VRFEASQYEYLPTKNVY 276
              K  + Q+DTAA+L+SSGTTG SKGVMLSHRN I+ ++ +  RF   Q          
Sbjct: 207 SRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT-------- 266

Query: 277 LAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALA 336
           +  +PM HI+G   F  GL++LG ++VV+ KFD+  ++ A++  + ++  +VPPI+ A+ 
Sbjct: 267 ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 326

Query: 337 RTAEKI-GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGTRGFN 396
             A +I   +   SL  V  G A  S++  + FV   P V  +QGYG+TESTA+    FN
Sbjct: 327 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 386

Query: 397 TKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEATTFTM 456
            +  + Y + GLLAPN E K+VD  TG  +   +TGEL +R P  MKGY  N EAT  T+
Sbjct: 387 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 446

Query: 457 DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTAVAAA 516
           D E WL TGD+ Y D DG+++VVDRLKE+IK  G+Q+AP +LEA+++ HPE+ D AV   
Sbjct: 447 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 506

Query: 517 KDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGKVLRR 571
            D + G+ P+A++V+K GS L++ +++ +VA+QV+PYKK+RKV F  SIPK+ +GK+LRR
Sbjct: 507 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 559

BLAST of CsGy3G026030 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 399.1 bits (1024), Expect = 6.3e-111
Identity = 223/548 (40.69%), Postives = 334/548 (60.95%), Query Frame = 0

Query: 39  PDTGICRSVHGY------RKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKEL 98
           P +G C+S   +        LP +   DV +FI S    H G +A ID++TG  +++ +L
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISS--QPHRGKTAFIDAATGQCLTFSDL 71

Query: 99  FPMVKSMASGL-HNLGISQGDVVLLMLPNSIFFPIIILGALYLGAVVTTMFPQSSSSEIK 158
           +  V  +A  L H +GI +GDVVL++ PNSIF P++ L  + LGAV TT    ++S EI 
Sbjct: 72  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 131

Query: 159 KRITDCNVRLAFAITQKIKNFEALGIKTIGVPENTNFNLMRSMGFSSFYELISGGSDLIK 218
           K+I D N  L F  T+++     + I  +   +     L      +S   ++   S+++K
Sbjct: 132 KQIADSNPTLVFT-TRQLAPKLPVAISVVLTDDEVYQEL------TSAIRVVGILSEMVK 191

Query: 219 KPV--------IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPT 278
           K          + Q+DTA +L+SSGTTG SKGV+ SHRN  + +  F+       + L  
Sbjct: 192 KEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKR 251

Query: 279 KNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPIL 338
            ++++  VPMFH YGL  F MG ++LGS+VV++ +F + D++ A+++ + T   + PP+L
Sbjct: 252 DDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVL 311

Query: 339 TALARTAEKI-GVHRFRSLKQVSCGAAASSKKTIDNFVHALPHVDFIQGYGMTESTAVGT 398
            A+   A+ I   +   SLK V CG A  SK+  + F+   P VD +QGY +TES   G 
Sbjct: 312 VAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGA 371

Query: 399 RGFNTKNARNYLSVGLLAPNTEAKVVDWVTGSSMPPGKTGELLLRGPGSMKGYLNNPEAT 458
              + + +R Y + G L  + EA++VD  TG  M   +TGEL L+GP   KGY  N EAT
Sbjct: 372 FTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEAT 431

Query: 459 TFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQIAPTDLEAVVITHPEVLDTA 518
             T++ E WL TGD+ Y D DG+L+VVDRLKE+IKYKG+Q+ P +LEA++ITHP++LD A
Sbjct: 432 NETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAA 491

Query: 519 VAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQQVAPYKKVRKVIFTESIPKSAAGK 571
           V    D+E G+ P+A+VV+K  S L++K V+D++++QVAPYKK+RKV F  SIPK+A+GK
Sbjct: 492 VIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGK 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84P243.3e-18158.034-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=... [more]
Q6YYZ21.9e-14452.004-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3... [more]
Q336M71.7e-12950.874-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2... [more]
M4IQR75.9e-12243.07Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1[more]
Q84P211.4e-12042.994-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_004144410.10.0100.004-coumarate--CoA ligase-like 6 [Cucumis sativus] >KGN58405.1 hypothetical protei... [more]
TYK21080.10.094.464-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa][more]
XP_008460452.10.092.75PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo][more]
KAA0068160.10.094.264-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa][more]
XP_038888724.10.086.934-coumarate--CoA ligase-like 6 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0L8X10.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1[more]
A0A5D3DC800.094.464-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CCH70.092.754-coumarate--CoA ligase-like 6 OS=Cucumis melo OX=3656 GN=LOC103499264 PE=4 SV=1[more]
A0A5A7VLT70.094.264-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A6J1HGK90.082.234-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT4G19010.12.3e-18258.03AMP-dependent synthetase and ligase family protein [more]
AT1G20510.11.0e-12142.99OPC-8:0 CoA ligase1 [more]
AT4G05160.15.8e-11742.43AMP-dependent synthetase and ligase family protein [more]
AT1G20480.14.3e-11240.81AMP-dependent synthetase and ligase family protein [more]
AT1G20500.16.3e-11140.69AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 474..574
e-value: 2.3E-29
score: 104.0
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 28..571
NoneNo IPR availablePANTHERPTHR24096:SF2614-COUMARATE--COA LIGASE-LIKE 6coord: 28..571
NoneNo IPR availableCDDcd059044CLcoord: 53..568
e-value: 0.0
score: 725.566
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 40..572
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 71..478
e-value: 1.1E-100
score: 337.2
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 49..473
e-value: 8.8E-117
score: 392.4
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 488..562
e-value: 2.5E-16
score: 60.4
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 223..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G026030.2CsGy3G026030.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006744 ubiquinone biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005777 peroxisome
molecular_function GO:0016405 CoA-ligase activity