CsGy3G025300 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G025300
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description26S proteasome regulatory subunit 4 homolog
LocationGy14Chr3: 25796593 .. 25800127 (+)
RNA-Seq ExpressionCsGy3G025300
SyntenyCsGy3G025300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAATTAAAGACAACAGTGATTCTTTTCTTGTTCAATGGGTCACCGGCGGACTGAATATTAGCTTCAATTGGGAGCCATTTCTCGGAATCTCTCTTTCAATTGCAAAAAAATGGGTCAGGGAACTCCAGGAGGTTTGAACCGGCAAGCCCCCGGCGACCGGAAGTCCGACGCCGATAAAAAGAAAGACAAAAAGTATGAACCGGCGGCGCCGCCAACTCGAGTTGGCCGCAAACAGCGGAAGCAGAAAGGCCCGGAGGCGGCTGCTCGACTCCCGACGGTTACGCCTCTTACGAAGTGCAGGCTGAGGCTTTTGAAGCTCGAACGTGTGAAGGATTACCTGCTTATGGAAGAGGAATTTGTCACTAATCAGGAGCGCCTTAAGCCGCAGGAGGAGAAGGCGGAGGAGGATCGCTCTAAGGTCGATGATCTGAGAGGATCTCCCATGAGTGTCGGGAATTTGGAGGAGTTGATTGATGAGAATCATGCCATCGTTTCCTCTTCTGTTGGGCCGGAGTATTACGTTGGTATTATGTCTTTTGTTGATAAAGATCAGCTTGAGCCTGGGTGTGCAATTTTGATGCACAATAAGGTATAGGGCTTTTGGTTTCTATTTGAAGATTTTGAATTTCTCTTGTTAACTTCTTTGTGCTTGATTTTTTGTTGGAGTAGATTTTATTTCCTCGGTGTGTTAAACTGTATGATTCTGAAAGTTAAGCTTGGGTAGTTTGTGTTGGAATTTTAGGGATTTTTTTCTTGATTGGGTGGGAACACAATGGGGCAATTTCTTAGCTTTGTCTTTTGAGTAGAAGAAAGAGGGAATGACTTAAATTGTTGATGGTATCTCAAGTTTAGTAATCGGGGTTCAGTTCATATAATGTTTCATTACGTGTTTGTGGAACCTAAAATGCTGGTTTATCTCGGAGGTACCTCTCTTTTGTTAAAAACGAGTGTATTGAGACTGGAAAATGGAACATCTCAGTACTAAAATTGTCCAGAGAACTTGACATTGTTGAGCTCTTTTATGAACTATAAGTCTCGTGTTTTAACTTTGAATGGCATGTGTTGGGAGTGATTTTGAAATGGTTAAAATCACTTGTCAGTTTTAAAATACTCCAAACATGTTGTCGATCTATATGAGCTTGAATTGTAAACTCTAAATTGCAGTTTTGATTTTATGAAATAATTTAGAAGTGTTAAGATCAAACATAAAAATGATTTTGAAGGATTAAGGACATGCTTGAAATTGATTTTGAATTTGATAGAATTGCAAACATGCCCAAAGAGTCATAGTTGGGTATCTGTGATGGAAACCACATAGCTAGCTGATGCTTTTTATGGCATTCTGCAAGTCTTTTAGTTTGTCTTTTTTGGAGTATCATCAAGACCCTGGTTGATCAATTTGCTTACTATCAACGGTTTGTTTAATAGGTACTTTCCGTTGTTGGACTTCTCCAAGATGAGGTTGATCCAATGGTGTCTGTGATGAAAGTTGAGAAGGCCCCTTTAGAGTCATATGCTGATATTGGTGGATTGGATGCGCAAATACAAGAGATCAAAGAAGCTGTTGAGTTGCCATTGACTCATCCTGAGTTATATGAGGACATTGGTATTAGGCCACCCAAAGGTGTGATACTCTATGGGGAACCAGGAACTGGCAAGACTTTGCTTGCTAAGGTGATTGTTTATTCTTCTTTCTCCCTTAAAAAACTTTGCTGTCCACTCATTCAGTAGATTTATAACCTATTCTGTTTTAAGTTGAAGTCACGCTTATGTAAACTTTTTATCATTCAGACTAATTTCTTTTGGTTCTCTATTCAACTTTAAGATAAAATTTTAAATTAGAAGCCATCTAAGAATATAGTTGTAGAGAAAACCTGGATCACCTTCTTTGGGATTGCCGGTTTGCGAGGTCTAATGGTTGTGCAATGATCAGGGAGTTCCTTCTCCATCTGCCTTCCATAGAGAAAGACCGTTTGTTGTGGTTTGTTGGAGTATGTGTTGTGGGATATTTGGGGGGAGAAAAACGAAATGTTGTTTAGAGATTTGGATAAGGACTCTAGTGAGGTTTGGTCCTTAGTGAGGTTTGAAGTTTTGCTGTGAGCTTTCGTTTCGAACTTCTTTTTGTAATTATTTATTGGTAATCGTATTTTCCCTCTTTTAGGCAGTGGCAAACTCAACTTCAGCCACTTTTCTGCGTGTGGTTGGGAGTGAACTCATTCAGAAATACTTGGGAGATGGTCCAAAGTTAGTAAGGGAACTCTTCAGGGTTGCTGATGATCTCTCACCTTCCATTGTTTTTATTGATGAGATTGATGCTGTCGGCACAAAGAGGTCAGTTTTTTACTTTCTTATTCTAAAAGAAGAAGACTTTTGTTTCTGATTCATAATCCCTACGTGCAGGTATGATGCTCATTCTGGAGGTGAACGTGAGATCCAAAGGACAATGTTGGAATTACTTAACCAATTGGATGGTTTTGATTCGAGAGGAGATGTCAAAGTGATACTTGCTACAAACAGAATTGAAAGTCTCGATCCAGCTTTGCTTCGACCCGGCCGAATAGATAGGAAGATTGAGTTTCCATTGCCTGATATAAAAACCAGGAGGCGCATTTTTCAGGTTTACTTTCCAATTGAATGCCATGTTAATGCGTGAACTTTGCATTAACTTATACCATTTGATAGTTCAGTTTGGTTAGATATCGTTTATCTTTATCTAACAACTTGACAACTCTGATATGTAGATACACACATCAAGGATGACATTAGCAGATGATGTGAACTTGGAGGAATTTGTTATGACCAAAGATGAATTTTCTGGTGCTGATATAAAGGCCATATGTACTGAAGCTGGACTGCTTGCTTTGAGAGAACGGCGTATGAAGGTAAGAGTTCCCTAAGTTTCTAAATGAAAGAGTTCGTTCCCTAAGTTTCTAAATGAAAGAGTTCCCTAGGTTTCTAAATGAATTATGATTCAAGAAAACTCTTCCACTTGAATGATCTATTAACTAAGCTTTTGAAAGCTCCGTTGAGGTAGGCTTCAAAAACTTACAATGGATGATTAAGCTCCAAAGTTTTTGGTCTATCAAACATCAAGAAGTATCGAAATCTACAGTACAGATAGAATGGGATTGAACAGTTTGTCCCTAGTCTGTTTCGTGTATTGTTACTCTTGGGGTGTAAGCTTAGGTTCTTTTTTATGTTGTCTTTTGGTTCAGGTCACACATGCCGACTTCAAGAAGGCAAAGGAAAAGGTTATGTACAAGAAGAAAGAAGGCGTGCCAGAAGGCTTATATATGTGATGAAGATTATTGTTAAGTGTGTTTGAAACTCGAAGGAATGCCTTCTTTCACGTTTTGGATTATGGAAATACTCTGTTCTCAGATTCTAATCAATATATATGTGTTTTCCATTATGAAGCTAATTTGTTACCAGCCTTTGTTGGATATGTATTTTATATGTTCTCCAAACTCTTCTGCTCATGTTTTAGAACTTGAAAGCATCAATTTTGAAGATAATCAGAAATTTCTCCCTC

mRNA sequence

CTCAAATTAAAGACAACAGTGATTCTTTTCTTGTTCAATGGGTCACCGGCGGACTGAATATTAGCTTCAATTGGGAGCCATTTCTCGGAATCTCTCTTTCAATTGCAAAAAAATGGGTCAGGGAACTCCAGGAGGTTTGAACCGGCAAGCCCCCGGCGACCGGAAGTCCGACGCCGATAAAAAGAAAGACAAAAAGTATGAACCGGCGGCGCCGCCAACTCGAGTTGGCCGCAAACAGCGGAAGCAGAAAGGCCCGGAGGCGGCTGCTCGACTCCCGACGGTTACGCCTCTTACGAAGTGCAGGCTGAGGCTTTTGAAGCTCGAACGTGTGAAGGATTACCTGCTTATGGAAGAGGAATTTGTCACTAATCAGGAGCGCCTTAAGCCGCAGGAGGAGAAGGCGGAGGAGGATCGCTCTAAGGTCGATGATCTGAGAGGATCTCCCATGAGTGTCGGGAATTTGGAGGAGTTGATTGATGAGAATCATGCCATCGTTTCCTCTTCTGTTGGGCCGGAGTATTACGTTGGTATTATGTCTTTTGTTGATAAAGATCAGCTTGAGCCTGGGTGTGCAATTTTGATGCACAATAAGGTACTTTCCGTTGTTGGACTTCTCCAAGATGAGGTTGATCCAATGGTGTCTGTGATGAAAGTTGAGAAGGCCCCTTTAGAGTCATATGCTGATATTGGTGGATTGGATGCGCAAATACAAGAGATCAAAGAAGCTGTTGAGTTGCCATTGACTCATCCTGAGTTATATGAGGACATTGGTATTAGGCCACCCAAAGGTGTGATACTCTATGGGGAACCAGGAACTGGCAAGACTTTGCTTGCTAAGGCAGTGGCAAACTCAACTTCAGCCACTTTTCTGCGTGTGGTTGGGAGTGAACTCATTCAGAAATACTTGGGAGATGGTCCAAAGTTAGTAAGGGAACTCTTCAGGGTTGCTGATGATCTCTCACCTTCCATTGTTTTTATTGATGAGATTGATGCTGTCGGCACAAAGAGGTATGATGCTCATTCTGGAGGTGAACGTGAGATCCAAAGGACAATGTTGGAATTACTTAACCAATTGGATGGTTTTGATTCGAGAGGAGATGTCAAAGTGATACTTGCTACAAACAGAATTGAAAGTCTCGATCCAGCTTTGCTTCGACCCGGCCGAATAGATAGGAAGATTGAGTTTCCATTGCCTGATATAAAAACCAGGAGGCGCATTTTTCAGATACACACATCAAGGATGACATTAGCAGATGATGTGAACTTGGAGGAATTTGTTATGACCAAAGATGAATTTTCTGGTGCTGATATAAAGGCCATATGTACTGAAGCTGGACTGCTTGCTTTGAGAGAACGGCGTATGAAGGTCACACATGCCGACTTCAAGAAGGCAAAGGAAAAGGTTATGTACAAGAAGAAAGAAGGCGTGCCAGAAGGCTTATATATGTGATGAAGATTATTGTTAAGTGTGTTTGAAACTCGAAGGAATGCCTTCTTTCACGTTTTGGATTATGGAAATACTCTGTTCTCAGATTCTAATCAATATATATGTGTTTTCCATTATGAAGCTAATTTGTTACCAGCCTTTGTTGGATATGTATTTTATATGTTCTCCAAACTCTTCTGCTCATGTTTTAGAACTTGAAAGCATCAATTTTGAAGATAATCAGAAATTTCTCCCTC

Coding sequence (CDS)

ATGGGTCAGGGAACTCCAGGAGGTTTGAACCGGCAAGCCCCCGGCGACCGGAAGTCCGACGCCGATAAAAAGAAAGACAAAAAGTATGAACCGGCGGCGCCGCCAACTCGAGTTGGCCGCAAACAGCGGAAGCAGAAAGGCCCGGAGGCGGCTGCTCGACTCCCGACGGTTACGCCTCTTACGAAGTGCAGGCTGAGGCTTTTGAAGCTCGAACGTGTGAAGGATTACCTGCTTATGGAAGAGGAATTTGTCACTAATCAGGAGCGCCTTAAGCCGCAGGAGGAGAAGGCGGAGGAGGATCGCTCTAAGGTCGATGATCTGAGAGGATCTCCCATGAGTGTCGGGAATTTGGAGGAGTTGATTGATGAGAATCATGCCATCGTTTCCTCTTCTGTTGGGCCGGAGTATTACGTTGGTATTATGTCTTTTGTTGATAAAGATCAGCTTGAGCCTGGGTGTGCAATTTTGATGCACAATAAGGTACTTTCCGTTGTTGGACTTCTCCAAGATGAGGTTGATCCAATGGTGTCTGTGATGAAAGTTGAGAAGGCCCCTTTAGAGTCATATGCTGATATTGGTGGATTGGATGCGCAAATACAAGAGATCAAAGAAGCTGTTGAGTTGCCATTGACTCATCCTGAGTTATATGAGGACATTGGTATTAGGCCACCCAAAGGTGTGATACTCTATGGGGAACCAGGAACTGGCAAGACTTTGCTTGCTAAGGCAGTGGCAAACTCAACTTCAGCCACTTTTCTGCGTGTGGTTGGGAGTGAACTCATTCAGAAATACTTGGGAGATGGTCCAAAGTTAGTAAGGGAACTCTTCAGGGTTGCTGATGATCTCTCACCTTCCATTGTTTTTATTGATGAGATTGATGCTGTCGGCACAAAGAGGTATGATGCTCATTCTGGAGGTGAACGTGAGATCCAAAGGACAATGTTGGAATTACTTAACCAATTGGATGGTTTTGATTCGAGAGGAGATGTCAAAGTGATACTTGCTACAAACAGAATTGAAAGTCTCGATCCAGCTTTGCTTCGACCCGGCCGAATAGATAGGAAGATTGAGTTTCCATTGCCTGATATAAAAACCAGGAGGCGCATTTTTCAGATACACACATCAAGGATGACATTAGCAGATGATGTGAACTTGGAGGAATTTGTTATGACCAAAGATGAATTTTCTGGTGCTGATATAAAGGCCATATGTACTGAAGCTGGACTGCTTGCTTTGAGAGAACGGCGTATGAAGGTCACACATGCCGACTTCAAGAAGGCAAAGGAAAAGGTTATGTACAAGAAGAAAGAAGGCGTGCCAGAAGGCTTATATATGTGA

Protein sequence

MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPLTKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADFKKAKEKVMYKKKEGVPEGLYM*
Homology
BLAST of CsGy3G025300 vs. ExPASy Swiss-Prot
Match: Q9SZD4 (26S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPT2A PE=1 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 8.8e-235
Identity = 419/445 (94.16%), Postives = 433/445 (97.30%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQG  GGLNRQ  GDRK D   KK+KK+EPAAPP RVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGPSGGLNRQ--GDRKPDGGDKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPS 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKC+LRLLKLER+KDYLLMEEEFV NQERLKPQEEKAEEDRSKVDDLRG+PMSVGNLEEL
Sbjct: 61  TKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGI+SFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGI+PPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTS+MTL++DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSKMTLSEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 446
           H DFKKAKEKVM+KKKEGVPEGLYM
Sbjct: 421 HPDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of CsGy3G025300 vs. ExPASy Swiss-Prot
Match: Q9SL67 (26S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPT2B PE=1 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 1.2e-234
Identity = 419/445 (94.16%), Postives = 434/445 (97.53%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQG  GGLNRQ  GDRK D  +KK+KK+EPAAPP RVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGPSGGLNRQ--GDRKPDGGEKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPS 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKC+LRLLKLER+KDYLLMEEEFV NQERLKPQEEKAEEDRSKVDDLRG+PMSVGNLEEL
Sbjct: 61  TKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGI+SFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGI+PPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DA+SGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DANSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTS+MTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 446
           H DFKKAKEKVM+KKKEGVPEGLYM
Sbjct: 421 HVDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of CsGy3G025300 vs. ExPASy Swiss-Prot
Match: P46466 (26S proteasome regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=TBP2 PE=2 SV=2)

HSP 1 Score: 800.4 bits (2066), Expect = 1.0e-230
Identity = 414/448 (92.41%), Postives = 432/448 (96.43%), Query Frame = 0

Query: 1   MGQGTPGGLNRQ--APGDRKSDAD-KKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTV 60
           MGQGTPGG+ +Q  APGDRK   D  KKD+K+EP A P+RVGRKQRKQKGPEAAARLP V
Sbjct: 1   MGQGTPGGMGKQGGAPGDRKPGGDGDKKDRKFEPPAAPSRVGRKQRKQKGPEAAARLPNV 60

Query: 61  TPLTKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNL 120
            PL+KCRLRLLKLERVKDYLLMEEEFV  QERL+P E+K EEDRSKVDDLRG+PMSVG+L
Sbjct: 61  APLSKCRLRLLKLERVKDYLLMEEEFVAAQERLRPTEDKTEEDRSKVDDLRGTPMSVGSL 120

Query: 121 EELIDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVS 180
           EE+IDE+HAIVSSSVGPEYYVGI+SFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVS
Sbjct: 121 EEIIDESHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVS 180

Query: 181 VMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGK 240
           VMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGK
Sbjct: 181 VMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGK 240

Query: 241 TLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGT 300
           TLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVAD+LSPSIVFIDEIDAVGT
Sbjct: 241 TLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADELSPSIVFIDEIDAVGT 300

Query: 301 KRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIE 360
           KRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIE
Sbjct: 301 KRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIE 360

Query: 361 FPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRM 420
           FPLPDIKTRRRIFQIHTS+MTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRM
Sbjct: 361 FPLPDIKTRRRIFQIHTSKMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRM 420

Query: 421 KVTHADFKKAKEKVMYKKKEGVPEGLYM 446
           KVTHADFKKAKEKVM+KKKEGVPEGLYM
Sbjct: 421 KVTHADFKKAKEKVMFKKKEGVPEGLYM 448

BLAST of CsGy3G025300 vs. ExPASy Swiss-Prot
Match: P48601 (26S proteasome regulatory subunit 4 OS=Drosophila melanogaster OX=7227 GN=Rpt2 PE=1 SV=2)

HSP 1 Score: 688.0 bits (1774), Expect = 7.3e-197
Identity = 344/431 (79.81%), Postives = 398/431 (92.34%), Query Frame = 0

Query: 15  GDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPLTKCRLRLLKLERVK 74
           G++K D DKK  KKYEP   PTRVG+K+R+ KGP+AA +LP VTP T+CRL+LLKLER+K
Sbjct: 12  GEKKDDKDKK--KKYEPPI-PTRVGKKKRRAKGPDAAMKLPQVTPHTRCRLKLLKLERIK 71

Query: 75  DYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSVGP 134
           DYL+ME+EF+ NQERLKPQ+EK EE+RSKVDDLRG+PMSVGNLEE+ID+NHAIVS+SVG 
Sbjct: 72  DYLMMEDEFIRNQERLKPQDEKNEEERSKVDDLRGTPMSVGNLEEIIDDNHAIVSTSVGS 131

Query: 135 EYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKAPLESYADIGG 194
           E+YV I+SFVDKDQLEPGC++L+++KV +VVG+L D+ DPMV+VMK+EKAP E+YADIGG
Sbjct: 132 EHYVSILSFVDKDQLEPGCSVLLNHKVHAVVGVLSDDTDPMVTVMKLEKAPQETYADIGG 191

Query: 195 LDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLLAKAVANSTSATFLR 254
           LD QIQEIKE+VELPLTHPE YE++GI+PPKGVILYG PGTGKTLLAKAVAN TSATFLR
Sbjct: 192 LDTQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLR 251

Query: 255 VVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTM 314
           VVGSELIQKYLGDGPKLVRELFRVA++ +PSIVFIDEIDAVGTKRYD++SGGEREIQRTM
Sbjct: 252 VVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAVGTKRYDSNSGGEREIQRTM 311

Query: 315 LELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHT 374
           LELLNQLDGFDSRGDVKVI+ATNRIE+LDPAL+RPGRIDRKIEFPLPD KT+RRIF IHT
Sbjct: 312 LELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKRRIFTIHT 371

Query: 375 SRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADFKKAKEKVMYK 434
           SRMTLA+DVNL E +M KD+ SGADIKAICTEAGL+ALRERRMKVT+ DFKK+KE V+Y+
Sbjct: 372 SRMTLAEDVNLSELIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKESVLYR 431

Query: 435 KKEGVPEGLYM 446
           KKEG PEGLY+
Sbjct: 432 KKEGTPEGLYL 439

BLAST of CsGy3G025300 vs. ExPASy Swiss-Prot
Match: P62191 (26S proteasome regulatory subunit 4 OS=Homo sapiens OX=9606 GN=PSMC1 PE=1 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 3.1e-195
Identity = 345/445 (77.53%), Postives = 401/445 (90.11%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQ   GG     PG  K D DK K KKYEP   PTRVG+K++K KGP+AA++LP VTP 
Sbjct: 1   MGQSQSGG---HGPGGGKKD-DKDKKKKYEPPV-PTRVGKKKKKTKGPDAASKLPLVTPH 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           T+CRL+LLKLER+KDYLLMEEEF+ NQE++KP EEK EE+RSKVDDLRG+PMSVG LEE+
Sbjct: 61  TQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEI 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           ID+NHAIVS+SVG E+YV I+SFVDKD LEPGC++L+++KV +V+G+L D+ DP+V+VMK
Sbjct: 121 IDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAP E+YADIGGLD QIQEIKE+VELPLTHPE YE++GI+PPKGVILYG PGTGKTLL
Sbjct: 181 VEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVAN TSATFLRVVGSELIQKYLGDGPKLVRELFRVA++ +PSIVFIDEIDA+GTKRY
Sbjct: 241 AKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           D++SGGEREIQRTMLELLNQLDGFDSRGDVKVI+ATNRIE+LDPAL+RPGRIDRKIEFPL
Sbjct: 301 DSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PD KT++RIFQIHTSRMTLADDV L++ +M KD+ SGADIKAICTEAGL+ALRERRMKVT
Sbjct: 361 PDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 446
           + DFKK+KE V+YKK+EG PEGLY+
Sbjct: 421 NEDFKKSKENVLYKKQEGTPEGLYL 440

BLAST of CsGy3G025300 vs. NCBI nr
Match: XP_004152012.1 (26S proteasome regulatory subunit 4 homolog B [Cucumis sativus] >XP_008447342.1 PREDICTED: 26S proteasome regulatory subunit 4 homolog B-like [Cucumis melo] >XP_038888689.1 26S proteasome regulatory subunit 4 homolog B-like [Benincasa hispida] >KGN58327.1 hypothetical protein Csa_017588 [Cucumis sativus])

HSP 1 Score: 859 bits (2220), Expect = 4.83e-314
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL
Sbjct: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. NCBI nr
Match: XP_022157874.1 (26S proteasome regulatory subunit 4 homolog B [Momordica charantia])

HSP 1 Score: 857 bits (2213), Expect = 5.63e-313
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQ+PGDRKSD DKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL
Sbjct: 1   MGQGTPGGLNRQSPGDRKSDGDKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. NCBI nr
Match: XP_022963682.1 (26S proteasome regulatory subunit 4 homolog B-like [Cucurbita moschata] >XP_023554101.1 26S proteasome regulatory subunit 4 homolog B-like [Cucurbita pepo subsp. pepo] >KAG6571568.1 26S proteasome regulatory subunit 4-like B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013546.1 26S proteasome regulatory subunit 4-like B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855 bits (2210), Expect = 1.61e-312
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQ+PGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGTPGGLNRQSPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPH 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. NCBI nr
Match: XP_022931149.1 (26S proteasome regulatory subunit 4 homolog B [Cucurbita moschata] >XP_022995776.1 26S proteasome regulatory subunit 4 homolog B [Cucurbita maxima] >XP_023533049.1 26S proteasome regulatory subunit 4 homolog B [Cucurbita pepo subsp. pepo] >KAG7036273.1 26S proteasome regulatory subunit 4-like B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855 bits (2209), Expect = 2.29e-312
Identity = 443/445 (99.55%), Postives = 443/445 (99.55%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQAPGDRKSD DKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGTPGGLNRQAPGDRKSDGDKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPH 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. NCBI nr
Match: TYJ98013.1 (26S proteasome regulatory subunit 4-like protein B-like [Cucumis melo var. makuwa])

HSP 1 Score: 854 bits (2207), Expect = 4.63e-312
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           M +GTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL
Sbjct: 1   MMRGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. ExPASy TrEMBL
Match: A0A0A0LC96 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G623950 PE=3 SV=1)

HSP 1 Score: 859 bits (2220), Expect = 2.34e-314
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL
Sbjct: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. ExPASy TrEMBL
Match: A0A1S3BGN2 (26S proteasome regulatory subunit 4 homolog B-like OS=Cucumis melo OX=3656 GN=LOC103489815 PE=3 SV=1)

HSP 1 Score: 859 bits (2220), Expect = 2.34e-314
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL
Sbjct: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. ExPASy TrEMBL
Match: A0A6J1DXT2 (26S proteasome regulatory subunit 4 homolog B OS=Momordica charantia OX=3673 GN=LOC111024484 PE=3 SV=1)

HSP 1 Score: 857 bits (2213), Expect = 2.72e-313
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQ+PGDRKSD DKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL
Sbjct: 1   MGQGTPGGLNRQSPGDRKSDGDKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. ExPASy TrEMBL
Match: A0A6J1HFW7 (26S proteasome regulatory subunit 4 homolog B-like OS=Cucurbita moschata OX=3662 GN=LOC111463935 PE=3 SV=1)

HSP 1 Score: 855 bits (2210), Expect = 7.81e-313
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQ+PGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGTPGGLNRQSPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPH 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. ExPASy TrEMBL
Match: A0A6J1K2V2 (26S proteasome regulatory subunit 4 homolog B OS=Cucurbita maxima OX=3661 GN=LOC111491209 PE=3 SV=1)

HSP 1 Score: 855 bits (2209), Expect = 1.11e-312
Identity = 443/445 (99.55%), Postives = 443/445 (99.55%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQGTPGGLNRQAPGDRKSD DKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGTPGGLNRQAPGDRKSDGDKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPH 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL
Sbjct: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445
           HADFKKAKEKVMYKKKEGVPEGLYM
Sbjct: 421 HADFKKAKEKVMYKKKEGVPEGLYM 445

BLAST of CsGy3G025300 vs. TAIR 10
Match: AT4G29040.1 (regulatory particle AAA-ATPase 2A )

HSP 1 Score: 813.9 bits (2101), Expect = 6.3e-236
Identity = 419/445 (94.16%), Postives = 433/445 (97.30%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQG  GGLNRQ  GDRK D   KK+KK+EPAAPP RVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGPSGGLNRQ--GDRKPDGGDKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPS 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKC+LRLLKLER+KDYLLMEEEFV NQERLKPQEEKAEEDRSKVDDLRG+PMSVGNLEEL
Sbjct: 61  TKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGI+SFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGI+PPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTS+MTL++DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSKMTLSEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 446
           H DFKKAKEKVM+KKKEGVPEGLYM
Sbjct: 421 HPDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of CsGy3G025300 vs. TAIR 10
Match: AT2G20140.1 (AAA-type ATPase family protein )

HSP 1 Score: 813.5 bits (2100), Expect = 8.2e-236
Identity = 419/445 (94.16%), Postives = 434/445 (97.53%), Query Frame = 0

Query: 1   MGQGTPGGLNRQAPGDRKSDADKKKDKKYEPAAPPTRVGRKQRKQKGPEAAARLPTVTPL 60
           MGQG  GGLNRQ  GDRK D  +KK+KK+EPAAPP RVGRKQRKQKGPEAAARLPTVTP 
Sbjct: 1   MGQGPSGGLNRQ--GDRKPDGGEKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPS 60

Query: 61  TKCRLRLLKLERVKDYLLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEEL 120
           TKC+LRLLKLER+KDYLLMEEEFV NQERLKPQEEKAEEDRSKVDDLRG+PMSVGNLEEL
Sbjct: 61  TKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLEEL 120

Query: 121 IDENHAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMK 180
           IDENHAIVSSSVGPEYYVGI+SFVDKDQLEPGC+ILMHNKVLSVVG+LQDEVDPMVSVMK
Sbjct: 121 IDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMK 180

Query: 181 VEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLL 240
           VEKAPLESYADIGGL+AQIQEIKEAVELPLTHPELYEDIGI+PPKGVILYGEPGTGKTLL
Sbjct: 181 VEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLL 240

Query: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300
           AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY
Sbjct: 241 AKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRY 300

Query: 301 DAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360
           DA+SGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL
Sbjct: 301 DANSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPL 360

Query: 361 PDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420
           PDIKTRRRIFQIHTS+MTLA+DVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT
Sbjct: 361 PDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVT 420

Query: 421 HADFKKAKEKVMYKKKEGVPEGLYM 446
           H DFKKAKEKVM+KKKEGVPEGLYM
Sbjct: 421 HVDFKKAKEKVMFKKKEGVPEGLYM 443

BLAST of CsGy3G025300 vs. TAIR 10
Match: AT5G58290.1 (regulatory particle triple-A ATPase 3 )

HSP 1 Score: 379.8 bits (974), Expect = 3.0e-105
Identity = 185/362 (51.10%), Postives = 271/362 (74.86%), Query Frame = 0

Query: 66  RLLKLERVKDYLLMEEEFVTN-QERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDEN 125
           RL  LER  ++  ++EE+V + Q+ LK +  +A+E+  ++  +   P+ +G   E++D+N
Sbjct: 33  RLKSLERQLEFTDIQEEYVKDEQKNLKRELLRAQEEVKRIQSV---PLVIGQFMEMVDQN 92

Query: 126 HAIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPMVSVMKVEKA 185
           + IV S+ G  YYV I+S ++++ L+P  ++ +H    ++V +L  E D  +S++   + 
Sbjct: 93  NGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEK 152

Query: 186 PLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGTGKTLLAKAV 245
           P  SY DIGG D Q QEI+EAVELPLTH ELY+ IGI PP+GV+LYG PGTGKT+LAKAV
Sbjct: 153 PDVSYNDIGGCDIQKQEIREAVELPLTHHELYKQIGIDPPRGVLLYGPPGTGKTMLAKAV 212

Query: 246 ANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHS 305
           AN T+A F+RVVGSE +QKYLG+GP++VR++FR+A + +P+I+FIDE+DA+ T R+DA +
Sbjct: 213 ANHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEVDAIATARFDAQT 272

Query: 306 GGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIK 365
           G +RE+QR ++ELLNQ+DGFD   +VKVI+ATNR ++LDPALLRPGR+DRKIEFPLPD +
Sbjct: 273 GADREVQRILMELLNQMDGFDQTVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRR 332

Query: 366 TRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHADF 425
            +R +FQ+ TS+M L+D+V+LE++V   D+ S A+I AIC EAG+ A+R+ R  +   DF
Sbjct: 333 QKRLVFQVCTSKMNLSDEVDLEDYVSRPDKISAAEIAAICQEAGMHAVRKNRYVILPKDF 391

Query: 426 KK 427
           +K
Sbjct: 393 EK 391

BLAST of CsGy3G025300 vs. TAIR 10
Match: AT1G09100.1 (26S proteasome AAA-ATPase subunit RPT5B )

HSP 1 Score: 335.1 bits (858), Expect = 8.6e-92
Identity = 178/381 (46.72%), Postives = 250/381 (65.62%), Query Frame = 0

Query: 77  LLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELID---ENHA------- 136
           +L EE   TN + L+  +EK +E++ K+   +  P  VGN+ E+++   E+ A       
Sbjct: 37  ILKEESQRTNLD-LESVKEKIKENQEKIKLNKQLPYLVGNIVEILEMSPEDDAEEDGANI 96

Query: 137 -----------IVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPM 196
                      ++ +S     ++ ++  VD D L+PG  + ++     ++  L  E D  
Sbjct: 97  DLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDTLKPGDLVGVNKDSYLILDTLPSEYDSR 156

Query: 197 VSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGT 256
           V  M+V++ P E Y DIGGL+ QIQE+ EA+ LP+TH E +E +GIRPPKGV+LYG PGT
Sbjct: 157 VKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKEQFEKLGIRPPKGVLLYGPPGT 216

Query: 257 GKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAV 316
           GKTL+A+A A  T+ATFL++ G +L+Q ++GDG KLVR+ F +A + SP I+FIDEIDA+
Sbjct: 217 GKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFLLAKEKSPCIIFIDEIDAI 276

Query: 317 GTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRK 376
           GTKR+D+   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRK
Sbjct: 277 GTKRFDSEVSGDREVQRTMLELLNQLDGFSSDDRIKVIAATNRADILDPALMRSGRLDRK 336

Query: 377 IEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRER 436
           IEFP P  + R RI QIH+ +M +  DVN EE   + D+F+GA +KA+C EAG+LALR  
Sbjct: 337 IEFPHPTEEARGRILQIHSRKMNVNADVNFEELARSTDDFNGAQLKAVCVEAGMLALRRD 396

BLAST of CsGy3G025300 vs. TAIR 10
Match: AT3G05530.1 (regulatory particle triple-A ATPase 5A )

HSP 1 Score: 334.3 bits (856), Expect = 1.5e-91
Identity = 174/381 (45.67%), Postives = 247/381 (64.83%), Query Frame = 0

Query: 77  LLMEEEFVTNQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDENH----------- 136
           +L E+   TN E     +EK +E++ K+   +  P  VGN+ E+++ N            
Sbjct: 38  ILKEDAQRTNLE-CDSYKEKIKENQEKIKLNKQLPYLVGNIVEILEMNPEDDAEEDGANI 97

Query: 137 ----------AIVSSSVGPEYYVGIMSFVDKDQLEPGCAILMHNKVLSVVGLLQDEVDPM 196
                      ++ +S     ++ ++  VD D L+PG  + ++     ++  L  E D  
Sbjct: 98  DLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDSLKPGDLVGVNKDSYLILDTLPSEYDSR 157

Query: 197 VSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIRPPKGVILYGEPGT 256
           V  M+V++ P E Y DIGGL+ QIQE+ EA+ LP+TH E +E +G+RPPKGV+LYG PGT
Sbjct: 158 VKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKERFEKLGVRPPKGVLLYGPPGT 217

Query: 257 GKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAV 316
           GKTL+A+A A  T+ATFL++ G +L+Q ++GDG KLVR+ F++A + +P I+FIDEIDA+
Sbjct: 218 GKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFQLAKEKAPCIIFIDEIDAI 277

Query: 317 GTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRK 376
           GTKR+D+   G+RE+QRTMLELLNQLDGF S   +KVI ATNR + LDPAL+R GR+DRK
Sbjct: 278 GTKRFDSEVSGDREVQRTMLELLNQLDGFSSDERIKVIAATNRADILDPALMRSGRLDRK 337

Query: 377 IEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRER 436
           IEFP P  + R RI QIH+ +M +  DVN EE   + D+F+GA +KA+C EAG+LALR  
Sbjct: 338 IEFPHPTEEARARILQIHSRKMNVHPDVNFEELARSTDDFNGAQLKAVCVEAGMLALRRD 397

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZD48.8e-23594.1626S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SL671.2e-23494.1626S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana OX=3702 GN... [more]
P464661.0e-23092.4126S proteasome regulatory subunit 4 homolog OS=Oryza sativa subsp. japonica OX=3... [more]
P486017.3e-19779.8126S proteasome regulatory subunit 4 OS=Drosophila melanogaster OX=7227 GN=Rpt2 P... [more]
P621913.1e-19577.5326S proteasome regulatory subunit 4 OS=Homo sapiens OX=9606 GN=PSMC1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004152012.14.83e-314100.0026S proteasome regulatory subunit 4 homolog B [Cucumis sativus] >XP_008447342.1 ... [more]
XP_022157874.15.63e-31399.5526S proteasome regulatory subunit 4 homolog B [Momordica charantia][more]
XP_022963682.11.61e-31299.5526S proteasome regulatory subunit 4 homolog B-like [Cucurbita moschata] >XP_0235... [more]
XP_022931149.12.29e-31299.5526S proteasome regulatory subunit 4 homolog B [Cucurbita moschata] >XP_022995776... [more]
TYJ98013.14.63e-31299.5526S proteasome regulatory subunit 4-like protein B-like [Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
A0A0A0LC962.34e-314100.00AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G623950 PE=3 SV... [more]
A0A1S3BGN22.34e-314100.0026S proteasome regulatory subunit 4 homolog B-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1DXT22.72e-31399.5526S proteasome regulatory subunit 4 homolog B OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1HFW77.81e-31399.5526S proteasome regulatory subunit 4 homolog B-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1K2V21.11e-31299.5526S proteasome regulatory subunit 4 homolog B OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G29040.16.3e-23694.16regulatory particle AAA-ATPase 2A [more]
AT2G20140.18.2e-23694.16AAA-type ATPase family protein [more]
AT5G58290.13.0e-10551.10regulatory particle triple-A ATPase 3 [more]
AT1G09100.18.6e-9246.7226S proteasome AAA-ATPase subunit RPT5B [more]
AT3G05530.11.5e-9145.67regulatory particle triple-A ATPase 5A [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 223..362
e-value: 3.6E-20
score: 83.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 180..360
e-value: 3.4E-68
score: 231.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 188..438
IPR00593726S proteasome regulatory subunit P45-likeTIGRFAMTIGR01242TIGR01242coord: 80..432
e-value: 2.1E-140
score: 465.9
NoneNo IPR availableGENE3D1.10.8.60coord: 365..439
e-value: 3.6E-26
score: 92.8
NoneNo IPR availableGENE3D2.40.50.140coord: 112..179
e-value: 2.7E-24
score: 86.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..110
NoneNo IPR availablePANTHERPTHR2307326S PROTEASOME REGULATORY SUBUNITcoord: 32..445
NoneNo IPR availablePANTHERPTHR23073:SF111BNAA08G13550D PROTEINcoord: 32..445
NoneNo IPR availableCDDcd00009AAAcoord: 193..360
e-value: 1.68552E-26
score: 102.609
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 227..360
e-value: 3.4E-40
score: 137.5
IPR032501Proteasomal ATPase OB C-terminal domainPFAMPF16450Prot_ATP_ID_OBcoord: 114..167
e-value: 5.0E-10
score: 39.3
IPR041569AAA ATPase, AAA+ lid domainPFAMPF17862AAA_lid_3coord: 382..426
e-value: 2.6E-10
score: 40.0
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 330..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G025300.2CsGy3G025300.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901800 positive regulation of proteasomal protein catabolic process
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0030163 protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0008540 proteasome regulatory particle, base subcomplex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0036402 proteasome-activating activity