CsGy3G017310 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G017310
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionBeta-carotene hydroxylase
LocationGy14Chr3: 13301128 .. 13304045 (-)
RNA-Seq ExpressionCsGy3G017310
SyntenyCsGy3G017310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGAAATAGAAAAAGGGCAAAGTTATTTGTTGAATGTGTTTTTGTGTATATAAGGGAAAGAAGGGTTGGAGATGGTCCAACAAAGCTCCTCTCATCGTAATTTTGCTCCCACACTTCAACTGAATAGAAAGAAACCAAAAAAAAAACTTGTGGTCTCTGTTTCTTCAAACTCCATTTTTAAACCCCTCTCCCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCCCTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCACTCTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCCATCTCCTTTTCTTTTTCCACCTTCTGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGCAGAAAAACTTTACTCACTGTCTGCGTACTCGTTGAGGGTCAAAATAGTTCCGGCAAGGTAGACAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCTCACGTTTCCGAAAGATTAGCTAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTACTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGTCCTCTCTTCTTCTTCTTCCTCTATCTACCTAGAAACATTAATTGCTTTGTTTTTTCTCGTTGGCGCCGCCTTCTTCTGATTGAATATTGAATTGCGGTTGTTTGATTTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCAATTTCCATTCATCTCTCTACTTTTCTTGATGAATTCCATATGCTTTAATCTGATTCTTCACAATCAATTTGCGATTAATCAGGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTTTGGCACTCTTCCTTATGGCATATGCACGAGGTTTCTAATCCTTCTTCACTATATAATTTCTTGATTTCTGAGCAAATTTTTGTATACATTAATCAGCTCTGAGGCTTAATTTCATACCAAACAAACAATCAAAAACCTCTTCTCTATAGATTTAACTATCGATCTCTCATGTTCCAAATAATTATTTTTACAATAAAACAGAGAAAATGGTTGAGGGGCTTTGACCATTATTGATGTTGAATGTTTTGATTATTACAACAGTCGCATCATAAACCAAGAGAAGGACCATTCGAACTGAACGATATTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCATACGGGTTCTTCCATAAAGGGCTTGTTCCTGGACTCTGCTTTGGCGCAGTAAATCTCTGAATTTCTCTCTCATTATCTTTGGAGAATCTCAGTTTGTTCATACTCCCAACTGTTTAATGTTTGTTTAATCACTGGGGATTAGTGCTAATAAGAGTGTTATAATGACGAAAATAGCCTTTCGAAAAGAAAGAAAAAAAGTTGTGGTATTGAGAAAGGGGTTTGACACGCTGGCGTGTTGTGGCAGGGTCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTAGGTCCCATCGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGGTAAACTTTTCTTCTCTTTTCCTATTTACCTTCACCCACTTGTTCTTAATTTTTTCCAATTATAGTCCATTTACGTATTTTTAAGTTCATTATAATCCTTAATTACTTGCTATACTTGCTACTTATTCTACATAATAAGTCTATTCTAGTTCGGATTAAATAGAAAAAGAAAATACATTATTTATAAATTTAAATCTTAATTTTGATACTGTGTTGACTTTCATTAATGACTTTTTTGTATATGGATCACTAGATGTAGATTCTACTAATTAACTATATGAAAAATGGGTTAAATAAAATCTTCTAGATGTCCTTATTTCTAAATTTAAGAATACCGAATAAGGTAAAAATATTATGATGAACTAAACATATAAATGTAGACTCAATAAATATGGCTTTAAATCAACCAACTATTAGATACCAATAATAGCTCAAATAATAAGATAGCCATGGAGTCTGAAAAGTTCTAGATGTGACGTTAGATTTTTTTTTTTTTTTTTTACAGTTAAAACTACTTTTACATTTTACCCGTATCTTAGAGTAAATCTAAATTTAAAATTGAAGCTTATGATTTTTCTTTTCCCCTTTTTCTCCTTTGCTTTAGCTTCACCATTCGGACAAGTTCAATGGTGTGCCATACGGGCTTTTTTTGGGTCCTAAGGTATGTTTCAGTTCATATATTCTTTAATTTTATTTAAAAGGGTTATTTACAAAAACATAACAAAGTTTCATATAACTTTCATATTTCATAGTGTATCAATAATAATCCTATTATTCTATTGGGTCTATCCGTAGTAAATATTAAATTTTGTAATATTTTGTAAATATTTAGATTGATTTTGTTATATTTAAAATTGTATTTTTTTTAAAAAAAAATTAAATGGTGGGTGTGCAGGAATTAGAGGAAGTGGGAGGGTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCGAAAATCAATAGATGGTTCTTGATCAACCAAATATTATGTGAAAATTAGAGAGGAAAAAAAAAAGAAGCTTTACTTGAGCAGTAACTTTTTTCTTTTTCTAATATTAAAAAGTAAAATATTATTTAGTTCAGAGCTTTTTCTTTTTCTATTATGTACGAAGTAAAGGAGTAAAAGAATGAGATAGTGAATTGGGTACACAATTAACAAATGTTTCACTCTACTTTTTCTTAATTTCTATATGAGTAATAAATAAAAATACACGT

mRNA sequence

GGAGAAATAGAAAAAGGGCAAAGTTATTTGTTGAATGTGTTTTTGTGTATATAAGGGAAAGAAGGGTTGGAGATGGTCCAACAAAGCTCCTCTCATCGTAATTTTGCTCCCACACTTCAACTGAATAGAAAGAAACCAAAAAAAAAACTTGTGGTCTCTGTTTCTTCAAACTCCATTTTTAAACCCCTCTCCCCGCCGTCTCTTTCTCCGAGAACCTTCCTCTTCTTCCACCGTCTTTTCACTTTTCACCCCTCTTCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCACTCTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCCATCTCCTTTTCTTTTTCCACCTTCTGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGCAGAAAAACTTTACTCACTGTCTGCGTACTCGTTGAGGGTCAAAATAGTTCCGGCAAGGTAGACAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCTCACGTTTCCGAAAGATTAGCTAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTACTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCAATTTCCATTCATCTCTCTACTTTTCTTGATGAATTCCATATGCTTTAATCTGATTCTTCACAATCAATTTGCGATTAATCAGGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTTTGGCACTCTTCCTTATGGCATATGCACGAGTCGCATCATAAACCAAGAGAAGGACCATTCGAACTGAACGATATTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCATACGGGTTCTTCCATAAAGGGCTTGTTCCTGGACTCTGCTTTGGCGCAGGTCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTAGGTCCCATCGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAATGGTGTGCCATACGGGCTTTTTTTGGGTCCTAAGGAATTAGAGGAAGTGGGAGGGTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCGAAAATCAATAGATGGTTCTTGATCAACCAAATATTATGTGAAAATTAGAGAGGAAAAAAAAAAGAAGCTTTACTTGAGCAGTAACTTTTTTCTTTTTCTAATATTAAAAAGTAAAATATTATTTAGTTCAGAGCTTTTTCTTTTTCTATTATGTACGAAGTAAAGGAGTAAAAGAATGAGATAGTGAATTGGGTACACAATTAACAAATGTTTCACTCTACTTTTTCTTAATTTCTATATGAGTAATAAATAAAAATACACGT

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCACTCTCCCCAAACCATTCCATCTCTTCCTTACTTCCTCCCATCTCTCCCCTAAACCTCCATCTCCTTTTCTTTTTCCACCTTCTGTCTTCCGGAACAGTAGATTCCAATGGAAGATGCGCAGAAAAACTTTACTCACTGTCTGCGTACTCGTTGAGGGTCAAAATAGTTCCGGCAAGGTAGACAATCTCTCCGATGAAGAATCGCCGATTGTAGTTCCTCAGATCCCATCGCCTCACGTTTCCGAAAGATTAGCTAGGAAGAAATCGGAGCGTTTCACTTATCTTGTTGCTGCGGTTATGTCTACTTTCGGAATTACATCCATGGCTGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCTGTTGGTGCCGCTGTACGTTTTCAATTTCCATTCATCTCTCTACTTTTCTTGATGAATTCCATATGCTTTAATCTGATTCTTCACAATCAATTTGCGATTAATCAGGTCGGCATGGAGTTCTGGGCAAGATGGGCTCATAGAGCTCTTTGGCACTCTTCCTTATGGCATATGCACGAGTCGCATCATAAACCAAGAGAAGGACCATTCGAACTGAACGATATTTTCGCCATAATCAACGCTGTGCCGGCCATAGCTCTTCTTTCATACGGGTTCTTCCATAAAGGGCTTGTTCCTGGACTCTGCTTTGGCGCAGGTCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTAGGTCCCATCGCCAACGTCCCTTACTTCAGAAAGGTTGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAATGGTGTGCCATACGGGCTTTTTTTGGGTCCTAAGGAATTAGAGGAAGTGGGAGGGTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATGGACGGCTCGAAAATCAATAGATGGTTCTTGA

Protein sequence

MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS*
Homology
BLAST of CsGy3G017310 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 7.5e-117
Identity = 209/309 (67.64%), Postives = 238/309 (77.02%), Query Frame = 0

Query: 25  PKPPSPFLFPPSVFR-NSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPIVVPQI 84
           P P +P ++P + F  N     + RR+   TVC ++E      + +   ++    +  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 85  PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  ++E+LARKKSERFTYLVAAVMS+FGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 145 SLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAINQVGMEFWARWAHRALWHSSLWHM 204
           +LSVGAA                               VGMEFWARWAH+ALWH+SLWHM
Sbjct: 147 ALSVGAA-------------------------------VGMEFWARWAHKALWHASLWHM 206

Query: 205 HESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMF 264
           HESHHKPREGPFELND+FAIINAVPAIALL YGFFHKGL+PGLCFGAGLGITVFGMAYMF
Sbjct: 207 HESHHKPREGPFELNDVFAIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMF 266

Query: 265 VHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELE 324
           VHDGLVHKRFPVGP+ANVPY RKVAAAH LHHS+KFNGVPYGLFLGPKELEEVGGLEELE
Sbjct: 267 VHDGLVHKRFPVGPVANVPYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELE 304

Query: 325 KEINRRIKW 333
           KE+NRR ++
Sbjct: 327 KEVNRRTRY 304

BLAST of CsGy3G017310 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-112
Identity = 214/320 (66.88%), Postives = 239/320 (74.69%), Query Frame = 0

Query: 20  SSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVEGQNSSGKVDNLSDEESPI- 79
           SS +S   P+  +FPP  F+      + R K  LTVC ++E +   GK+   SD++    
Sbjct: 30  SSPVSTSTPTLVVFPP--FKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVASDDDDGAG 89

Query: 80  -VVPQ------IPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMAVYYRFYWQMEG 139
            V  Q        +  ++++LARKKSERFTYLVAAVMS+FGITSMAV++VYYRF WQMEG
Sbjct: 90  EVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQMEG 149

Query: 140 GEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAINQVGMEFWARWA 199
           GEIP SEMFGTF+LSVGAA                               VGMEFWARWA
Sbjct: 150 GEIPLSEMFGTFALSVGAA-------------------------------VGMEFWARWA 209

Query: 200 HRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAG 259
           H ALWH+SLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGL+PGLCFGAG
Sbjct: 210 HEALWHASLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAG 269

Query: 260 LGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPK 319
           LGITVFGMAYMFVHDGLVHKRFPVGPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPK
Sbjct: 270 LGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPK 316

Query: 320 ELEEVGGLEELEKEINRRIK 332
           ELEEVGGL+ LE EINRR K
Sbjct: 330 ELEEVGGLQVLEMEINRRTK 316

BLAST of CsGy3G017310 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 4.4e-109
Identity = 216/348 (62.07%), Postives = 246/348 (70.69%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPK---PPSPFLFPPSVFRNSRFQWKMRRKTLLTVCV 60
           MAAGLS A   KP H   +SS    +   P S   F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 61  LVEGQNSSGKVDNLSDEESPIVVPQIPSPH----VSERLARKKSERFTYLVAAVMSTFGI 120
           +VE +  +  ++N    ES      I + +    ++E+L RKKSER TYL+AA++S+FGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAHRALWH+SLW+MHESHHKPREGPFELND+FAI+NA PAI LL
Sbjct: 181 --------VGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           SYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDGLVHKRFPVGPIA+VPY RKVAAAHQL
Sbjct: 241 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 300

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           HH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+RRIK   + S  GS
Sbjct: 301 HHTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYKKASGSGS 306

BLAST of CsGy3G017310 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.2e-108
Identity = 211/333 (63.36%), Postives = 241/333 (72.37%), Query Frame = 0

Query: 1   MAAGLSA-ATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 60
           MAAGLS  A   KP +    SS  +  P S  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EGQNSSGKVDNLSDEESPIVVPQI-PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAV 120
           E +  S  +D+ +  ES     +I  +  + ++  +KKSERFTYL+AAVMS+FGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFA 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAA                            
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAA---------------------------- 180

Query: 181 INQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFF 240
              VGMEFWARWAHRALWH SLW+MHESHHKPREG FELND+FAI NAVPAI LL YGF 
Sbjct: 181 ---VGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFL 240

Query: 241 HKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDK 300
           +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQLHH+DK
Sbjct: 241 NKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDK 294

Query: 301 FNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           F GVPYGLFLGPKE+EEVGG EELEKEI+RRIK
Sbjct: 301 FKGVPYGLFLGPKEVEEVGGKEELEKEISRRIK 294

BLAST of CsGy3G017310 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 387.1 bits (993), Expect = 2.0e-106
Identity = 210/353 (59.49%), Postives = 245/353 (69.41%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHL----FLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVC 60
           MAA +S +   +   L    F    + +  PP  F  P +   N     + RRK  L  C
Sbjct: 1   MAAEISISASSRAICLQRNPFPAPKYFATAPPLLFFSPLTC--NLDAILRSRRKPRLAAC 60

Query: 61  VLVEGQN----SSGK----------VDNLSDEESPIVVPQIPSPHVSERLARKKSERFTY 120
            +++        SGK          ++  ++EE  + V       ++E+ ARKKSERFTY
Sbjct: 61  FVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEEKSLAV------RLAEKFARKKSERFTY 120

Query: 121 LVAAVMSTFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLF 180
           LVAAVMS+ GITSMAV++VYYRF WQMEGGE+PFSEMF TF+L+ GAA            
Sbjct: 121 LVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAA------------ 180

Query: 181 LMNSICFNLILHNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFA 240
                              +GME+WARWAHRALWH+SLWHMHESHH+PREGPFELNDIFA
Sbjct: 181 -------------------IGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFA 240

Query: 241 IINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP 300
           IINAVPAIALLS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP
Sbjct: 241 IINAVPAIALLSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP 300

Query: 301 YFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAR 336
           YF++VAAAHQLHHSDKF+GVPYGLFLGPKELEEVG LEELEKE+NRRIK + R
Sbjct: 301 YFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIKSSKR 314

BLAST of CsGy3G017310 vs. NCBI nr
Match: XP_004140758.1 (beta-carotene hydroxylase 2, chloroplastic [Cucumis sativus] >KGN57410.1 hypothetical protein Csa_009664 [Cucumis sativus])

HSP 1 Score: 621 bits (1602), Expect = 7.06e-224
Identity = 310/341 (90.91%), Postives = 310/341 (90.91%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
           GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 310

BLAST of CsGy3G017310 vs. NCBI nr
Match: NP_001315399.1 (beta-carotene hydroxylase 2, chloroplastic [Cucumis melo] >AID51467.1 beta-carotene hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 588 bits (1515), Expect = 1.28e-210
Identity = 295/341 (86.51%), Postives = 301/341 (88.27%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA+MS+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

BLAST of CsGy3G017310 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 587 bits (1513), Expect = 2.58e-210
Identity = 292/341 (85.63%), Postives = 299/341 (87.68%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAA +PKP HL LTSSHLSPKP  PFLFPPSVFRNSR QWKMRR+TL TVCVLVE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V+NLSDE SPI+VPQIPSPHVSERLARKKSERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELND+FAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKW A KSIDGS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWAAPKSIDGS 310

BLAST of CsGy3G017310 vs. NCBI nr
Match: KAA0033696.1 (beta-carotene hydroxylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 587 bits (1512), Expect = 3.67e-210
Identity = 294/341 (86.22%), Postives = 301/341 (88.27%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA++S+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAILSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

BLAST of CsGy3G017310 vs. NCBI nr
Match: KAG7014750.1 (Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 562 bits (1449), Expect = 1.46e-200
Identity = 282/341 (82.70%), Postives = 295/341 (86.51%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA  PKP HLFL SSHLSPKP +PFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QN+SG+V++LSDEE PIVVPQIPSP+V++RLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  DQNNSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELND+FAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGG EELEKEINRRIK T  KSI+GS
Sbjct: 301 GVPYGLFLGPKELEEVGGQEELEKEINRRIKLTTTKSINGS 310

BLAST of CsGy3G017310 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 3.42e-224
Identity = 310/341 (90.91%), Postives = 310/341 (90.91%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
           GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 310

BLAST of CsGy3G017310 vs. ExPASy TrEMBL
Match: A0A1S3AZ37 (beta-carotene hydroxylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484113 PE=3 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 6.20e-211
Identity = 295/341 (86.51%), Postives = 301/341 (88.27%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA+MS+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

BLAST of CsGy3G017310 vs. ExPASy TrEMBL
Match: A0A088CIY4 (Beta-carotene hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=CHXB PE=2 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 6.20e-211
Identity = 295/341 (86.51%), Postives = 301/341 (88.27%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA+MS+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAIMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

BLAST of CsGy3G017310 vs. ExPASy TrEMBL
Match: A0A5A7SUR5 (Beta-carotene hydroxylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001660 PE=3 SV=1)

HSP 1 Score: 587 bits (1512), Expect = 1.78e-210
Identity = 294/341 (86.22%), Postives = 301/341 (88.27%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA LSAA LPKPFHLFL+SSHLS KPP+PFLFPPSVF NSRFQWKMRRKTLLTVCVLVE
Sbjct: 1   MAAALSAALLPKPFHLFLSSSHLSLKPPTPFLFPPSVFPNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V +LSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAA++S+FGITSMAVMA
Sbjct: 61  DQNSSGEVHSLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAILSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTA KS  GS
Sbjct: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTAPKSNYGS 310

BLAST of CsGy3G017310 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 1.00e-200
Identity = 281/341 (82.40%), Postives = 294/341 (86.22%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60
           MAA +S A  P+P HLFL SSHLSPKP +PFLFPPSVFRNSR Q K+RRKTL TVCVLVE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120
            QNSSG+V++LSDEE PIVVPQIPSP+V++RLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFAIN 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV                             
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAV----------------------------- 180

Query: 181 QVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFFHK 240
             GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELND+FAIINAVPAIALLSYGFFHK
Sbjct: 181 --GMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHK 240

Query: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300
           GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN
Sbjct: 241 GLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDKFN 300

Query: 301 GVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 341
           GVPYGLFLGPKELEEVGG EELEKEINRRIK T  KSIDGS
Sbjct: 301 GVPYGLFLGPKELEEVGGQEELEKEINRRIKLTTTKSIDGS 310

BLAST of CsGy3G017310 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 396.0 bits (1016), Expect = 3.1e-110
Identity = 216/348 (62.07%), Postives = 246/348 (70.69%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPK---PPSPFLFPPSVFRNSRFQWKMRRKTLLTVCV 60
           MAAGLS A   KP H   +SS    +   P S   F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 61  LVEGQNSSGKVDNLSDEESPIVVPQIPSPH----VSERLARKKSERFTYLVAAVMSTFGI 120
           +VE +  +  ++N    ES      I + +    ++E+L RKKSER TYL+AA++S+FGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAHRALWH+SLW+MHESHHKPREGPFELND+FAI+NA PAI LL
Sbjct: 181 --------VGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 240

Query: 241 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQL 300
           SYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDGLVHKRFPVGPIA+VPY RKVAAAHQL
Sbjct: 241 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 300

Query: 301 HHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKWTARKSIDGS 342
           HH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+RRIK   + S  GS
Sbjct: 301 HHTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYKKASGSGS 306

BLAST of CsGy3G017310 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 393.7 bits (1010), Expect = 1.6e-109
Identity = 211/333 (63.36%), Postives = 241/333 (72.37%), Query Frame = 0

Query: 1   MAAGLSA-ATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 60
           MAAGLS  A   KP +    SS  +  P S  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EGQNSSGKVDNLSDEESPIVVPQI-PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAV 120
           E +  S  +D+ +  ES     +I  +  + ++  +KKSERFTYL+AAVMS+FGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFA 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAA                            
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAA---------------------------- 180

Query: 181 INQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFF 240
              VGMEFWARWAHRALWH SLW+MHESHHKPREG FELND+FAI NAVPAI LL YGF 
Sbjct: 181 ---VGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFL 240

Query: 241 HKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDK 300
           +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQLHH+DK
Sbjct: 241 NKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDK 294

Query: 301 FNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           F GVPYGLFLGPKE+EEVGG EELEKEI+RRIK
Sbjct: 301 FKGVPYGLFLGPKEVEEVGGKEELEKEISRRIK 294

BLAST of CsGy3G017310 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 139/257 (54.09%), Postives = 163/257 (63.42%), Query Frame = 0

Query: 1   MAAGLSAATLPKPFHLFLTSSHLSPK---PPSPFLFPPSVFRNSRFQWKMRRKTLLTVCV 60
           MAAGLS A   KP H   +SS    +   P S   F PS+ R  RF          +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPSL-RFKRF----------SVCY 60

Query: 61  LVEGQNSSGKVDNLSDEESPIVVPQIPSPH----VSERLARKKSERFTYLVAAVMSTFGI 120
           +VE +  +  ++N    ES      I + +    ++E+L RKKSER TYL+AA++S+FGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLIL 180
           TSMAVMAVYYRF WQMEGGEI   EMFGTF+LSVGAA                       
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAA----------------------- 180

Query: 181 HNQFAINQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALL 240
                   VGMEFWARWAHRALWH+SLW+MHESHHKPREGPFELND+FAI+NA PAI LL
Sbjct: 181 --------VGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 215

Query: 241 SYGFFHKGLVPGLCFGA 251
           SYGFF+KGLVPGLCFGA
Sbjct: 241 SYGFFNKGLVPGLCFGA 215

BLAST of CsGy3G017310 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 235.7 bits (600), Expect = 5.4e-62
Identity = 151/333 (45.35%), Postives = 178/333 (53.45%), Query Frame = 0

Query: 1   MAAGLSA-ATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLV 60
           MAAGLS  A   KP +    SS  +  P S  +FPPS+  N       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EGQNSSGKVDNLSDEESPIVVPQI-PSPHVSERLARKKSERFTYLVAAVMSTFGITSMAV 120
           E +  S  +D+ +  ES     +I  +  + ++  +KKSERFTYL+AAVMS+FGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVRFQFPFISLLFLMNSICFNLILHNQFA 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGA                             
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGA----------------------------- 180

Query: 181 INQVGMEFWARWAHRALWHSSLWHMHESHHKPREGPFELNDIFAIINAVPAIALLSYGFF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 225

Query: 241 HKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHQLHHSDK 300
                      AGLGIT+FGMAYMFVHDGLVHKRFPVGPIANVPY RKVAAAHQLHH+DK
Sbjct: 241 -----------AGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDK 225

Query: 301 FNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 332
           F GVPYGLFLGPKE+EEVGG EELEKEI+RRIK
Sbjct: 301 FKGVPYGLFLGPKEVEEVGGKEELEKEISRRIK 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498147.5e-11767.64Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=... [more]
B3SGL01.9e-11266.88Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=... [more]
Q9SZZ84.4e-10962.07Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LTG02.2e-10863.36Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498152.0e-10659.49Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=... [more]
Match NameE-valueIdentityDescription
XP_004140758.17.06e-22490.91beta-carotene hydroxylase 2, chloroplastic [Cucumis sativus] >KGN57410.1 hypothe... [more]
NP_001315399.11.28e-21086.51beta-carotene hydroxylase 2, chloroplastic [Cucumis melo] >AID51467.1 beta-carot... [more]
XP_038877448.12.58e-21085.63beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida][more]
KAA0033696.13.67e-21086.22beta-carotene hydroxylase 2 [Cucumis melo var. makuwa][more]
KAG7014750.11.46e-20082.70Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argy... [more]
Match NameE-valueIdentityDescription
A0A0A0L6063.42e-22490.91Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3AZ376.20e-21186.51beta-carotene hydroxylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034841... [more]
A0A088CIY46.20e-21186.51Beta-carotene hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=CHXB PE=2 SV... [more]
A0A5A7SUR51.78e-21086.22Beta-carotene hydroxylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1E4L51.00e-20082.40beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT4G25700.13.1e-11062.07beta-hydroxylase 1 [more]
AT5G52570.11.6e-10963.36beta-carotene hydroxylase 2 [more]
AT4G25700.21.1e-6254.09beta-hydroxylase 1 [more]
AT5G52570.25.4e-6245.35beta-carotene hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 180..307
e-value: 2.8E-13
score: 50.4
IPR045019Beta-carotene hydroxylase-likePANTHERPTHR31899BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTICcoord: 11..155
coord: 181..333
NoneNo IPR availablePANTHERPTHR31899:SF10BETA-CAROTENE HYDROXYLASEcoord: 11..155
coord: 181..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G017310.2CsGy3G017310.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity