CsGy3G015450 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G015450
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptiondiacylglycerol O-acyltransferase 3, cytosolic-like
LocationGy14Chr3: 11441246 .. 11442826 (-)
RNA-Seq ExpressionCsGy3G015450
SyntenyCsGy3G015450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTCCTTCAAATCTTCAATCAATCAATCAATAATTTCGAATACTTTTTCCCCAAATCCATGGAGGTCTACGGCACCGTTTCCGGCCAGCTTCCTGGCCTCTTCTGCCTCGATTTTTCTCAATCCGTTCGAGTCTCCGACCTAGATACCCGTCGGGGCCCCTCTACCCTGTCCGTTTCCGGTCGTTCGGTTGGTACTGAAACTCGTGCTTTGGGGCTCGTTGGTCGTCGGAGAATGGGTTCTTCTAGGTTCTGCGATGACGGTTATTTGCGGTATTATGTTGGGCCGACTTGTCATGGGGGGAATGTGAAGAAGGAGAAGGAGGCGGTGAAGAAGAAATTGAAATTGTTGAAAGGATTGTCTGCCACGGATTATGAATCGAGCTTGTTGTTTCGTTTCGATCATGGTTCTATTGAAGAATTTCAGTCGGATCGCTTTTCTGTAAGTTGAAAATTTCTGGAATCCGCCTACTAATTCATTCCTGTTTGCTTGCGCCACTTTATCTATACCGGTTGAATTAAATTGGAGTTGGACTTGAAATTTCTTCGCCATGGAATTCTTCTGTTTTCTCTCGATCATCATGAATGATTCCTTGGTGTATTTATTTTGGCTGATAACATTCATTAATCAGATTGTTGTTTCCTTGATTGCAGATTGAGGAAAGAAGAGAAGCATTGACAAGACAACTCCAACAGCTAAAATCAGAAGAGAAAGAACAGAAAAGAAAAAAGAAGCTAGAAAAGGCCAAGCTAAAGGCAGCTAGAACACAAAACATTCACGACTCAACATCTTCATCCTCTGAATCCAGTGACAATGAAGGCCATATGAACACCACAAGCTACCGATTAAAGAAAGCGCTTTCACAGCCATTCCCAGATCAATACCAGGCCAACACCATCCATGTATCAACATTGCCTCTTCCATTGCAAACCCAACTTCTCAATTCGAAAACCGAAAACATCGCAGTAACCCATTCGACGTCTGTAGGGAGGATTCAAGTGTGCATGGGAAACAAGTGCAAGAAGGCAGGAGCAGCAGCATTGATGGAAGAATTTGGGAGGGTGATGGGGGATGAAGCTGCTGTTTGTGGGTGTAAATGTATGGGAAAATGTAGAGATGGACCAAATGTGAGAGTTTTGGGTTCTATGGAGATGCAAATTCCTTTGTGCATTGGAGTTGGGGTGGAAGATGTTGGTAGAATTGTAGCTGAGTATCTTGGACAAGAAGGGGGACAGACACAATCAAGGTTAGCACCTGCAGCAATTTAAATTGATACTTTTCATAGAAGTCAAATTTTAGTGCAGTTTATTTGATGAGTTTGTACAAAGTTAATCATCCTCTTTTTTAGTTATACTAAGGTGGGTTGGGATAGAGAAGTTTTTAGACCTAAACCAACTATTAGGATTAAAAAAAATTTCATCCATTGGAGCTAGAGGGTTGTTTCAATCTCAATTCCATCAAGGAATTTGAGTTGGGTTAGTTGATTTAGGTGTTTCAAATTATTTGTACCCATTTGTGAATAAAATATTGCAATTAGAAGTTCTTTTAATGCTTATTTACTTGTTTTATCATTGTGTTAAA

mRNA sequence

CTTTTCCTTCAAATCTTCAATCAATCAATCAATAATTTCGAATACTTTTTCCCCAAATCCATGGAGGTCTACGGCACCGTTTCCGGCCAGCTTCCTGGCCTCTTCTGCCTCGATTTTTCTCAATCCGTTCGAGTCTCCGACCTAGATACCCGTCGGGGCCCCTCTACCCTGTCCGTTTCCGGTCGTTCGGTTGGTACTGAAACTCGTGCTTTGGGGCTCGTTGGTCGTCGGAGAATGGGTTCTTCTAGGTTCTGCGATGACGGTTATTTGCGGTATTATGTTGGGCCGACTTGTCATGGGGGGAATGTGAAGAAGGAGAAGGAGGCGGTGAAGAAGAAATTGAAATTGTTGAAAGGATTGTCTGCCACGGATTATGAATCGAGCTTGTTGTTTCGTTTCGATCATGGTTCTATTGAAGAATTTCAGTCGGATCGCTTTTCTATTGAGGAAAGAAGAGAAGCATTGACAAGACAACTCCAACAGCTAAAATCAGAAGAGAAAGAACAGAAAAGAAAAAAGAAGCTAGAAAAGGCCAAGCTAAAGGCAGCTAGAACACAAAACATTCACGACTCAACATCTTCATCCTCTGAATCCAGTGACAATGAAGGCCATATGAACACCACAAGCTACCGATTAAAGAAAGCGCTTTCACAGCCATTCCCAGATCAATACCAGGCCAACACCATCCATGTATCAACATTGCCTCTTCCATTGCAAACCCAACTTCTCAATTCGAAAACCGAAAACATCGCAGTAACCCATTCGACGTCTGTAGGGAGGATTCAAGTGTGCATGGGAAACAAGTGCAAGAAGGCAGGAGCAGCAGCATTGATGGAAGAATTTGGGAGGGTGATGGGGGATGAAGCTGCTGTTTGTGGGTGTAAATGTATGGGAAAATGTAGAGATGGACCAAATGTGAGAGTTTTGGGTTCTATGGAGATGCAAATTCCTTTGTGCATTGGAGTTGGGGTGGAAGATGTTGGTAGAATTGTAGCTGAGTATCTTGGACAAGAAGGGGGACAGACACAATCAAGGTTAGCACCTGCAGCAATTTAAATTGATACTTTTCATAGAAGTCAAATTTTAGTGCAGTTTATTTGATGAGTTTGTACAAAGTTAATCATCCTCTTTTTTAGTTATACTAAGGTGGGTTGGGATAGAGAAGTTTTTAGACCTAAACCAACTATTAGGATTAAAAAAAATTTCATCCATTGGAGCTAGAGGGTTGTTTCAATCTCAATTCCATCAAGGAATTTGAGTTGGGTTAGTTGATTTAGGTGTTTCAAATTATTTGTACCCATTTGTGAATAAAATATTGCAATTAGAAGTTCTTTTAATGCTTATTTACTTGTTTTATCATTGTGTTAAA

Coding sequence (CDS)

ATGGAGGTCTACGGCACCGTTTCCGGCCAGCTTCCTGGCCTCTTCTGCCTCGATTTTTCTCAATCCGTTCGAGTCTCCGACCTAGATACCCGTCGGGGCCCCTCTACCCTGTCCGTTTCCGGTCGTTCGGTTGGTACTGAAACTCGTGCTTTGGGGCTCGTTGGTCGTCGGAGAATGGGTTCTTCTAGGTTCTGCGATGACGGTTATTTGCGGTATTATGTTGGGCCGACTTGTCATGGGGGGAATGTGAAGAAGGAGAAGGAGGCGGTGAAGAAGAAATTGAAATTGTTGAAAGGATTGTCTGCCACGGATTATGAATCGAGCTTGTTGTTTCGTTTCGATCATGGTTCTATTGAAGAATTTCAGTCGGATCGCTTTTCTATTGAGGAAAGAAGAGAAGCATTGACAAGACAACTCCAACAGCTAAAATCAGAAGAGAAAGAACAGAAAAGAAAAAAGAAGCTAGAAAAGGCCAAGCTAAAGGCAGCTAGAACACAAAACATTCACGACTCAACATCTTCATCCTCTGAATCCAGTGACAATGAAGGCCATATGAACACCACAAGCTACCGATTAAAGAAAGCGCTTTCACAGCCATTCCCAGATCAATACCAGGCCAACACCATCCATGTATCAACATTGCCTCTTCCATTGCAAACCCAACTTCTCAATTCGAAAACCGAAAACATCGCAGTAACCCATTCGACGTCTGTAGGGAGGATTCAAGTGTGCATGGGAAACAAGTGCAAGAAGGCAGGAGCAGCAGCATTGATGGAAGAATTTGGGAGGGTGATGGGGGATGAAGCTGCTGTTTGTGGGTGTAAATGTATGGGAAAATGTAGAGATGGACCAAATGTGAGAGTTTTGGGTTCTATGGAGATGCAAATTCCTTTGTGCATTGGAGTTGGGGTGGAAGATGTTGGTAGAATTGTAGCTGAGTATCTTGGACAAGAAGGGGGACAGACACAATCAAGGTTAGCACCTGCAGCAATTTAA

Protein sequence

MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMGSSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEEFQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSDNEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGRIQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCIGVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI*
Homology
BLAST of CsGy3G015450 vs. ExPASy Swiss-Prot
Match: Q9C5W0 (Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 2.9e-33
Identity = 137/367 (37.33%), Postives = 188/367 (51.23%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEV G V  Q+P   C+    S  V+ L   R  S  S + R+VG  TR      R  + 
Sbjct: 1   MEVSGVVLRQIP---CV---SSGSVAGL---RLVSEFSGNTRTVGFRTRRF----RGIVC 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGN------VKKEKEAVKKKLKLLKGLSAT-DYESSLLFRF 120
           ++ F D G++ YY+ PT  G        ++KEK+A+KKK K+LK LS   D  SS+ F  
Sbjct: 61  NNEFADKGHVNYYIEPTRCGEEKEKVKVMEKEKKALKKKAKVLKSLSKNLDMFSSIGFGL 120

Query: 121 D--HGSIEEFQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDS 180
           D   G + E Q+   +I E  E L +QL+QLK+EEK  K+++K EKAK KA +     DS
Sbjct: 121 DPEAGLVGEIQTK--TISEATEILVKQLEQLKAEEKILKKQRKEEKAKAKAMKKMTEMDS 180

Query: 181 -TSSSSESSDNE---GHMNTTSYRLKKALSQPFPDQYQANTIHVSTLP------------ 240
            +SSSSESSD++   G +   S    KA     P Q +A    V+TLP            
Sbjct: 181 ESSSSSESSDSDCDKGKVVDMSSLRNKAKPVLEPLQPEAT---VATLPRIQEDAISCKNT 240

Query: 241 -----LPLQTQLLNSKTENIAVTHST---------SVGRIQVCMGNKCKKAGAAALMEEF 300
                + LQT  +     N   T  T          + R++VCMG KCK++G A L++EF
Sbjct: 241 SEALQIALQTSTIFPSMANPGQTLKTVEAVSVVGLPLNRVEVCMGGKCKRSGGALLLDEF 300

Query: 301 GRVM-GDEAAVCGCKCMGKCRDGPNVRVLGSMEMQI---------PLCIGVGVEDVGRIV 319
            R M G E +   CKCMGKCRDGPNVRV+   +  +          LC+GVG++DV  IV
Sbjct: 301 QRAMTGFEGSAVACKCMGKCRDGPNVRVVKETDAVMTDSVRTPSKTLCVGVGLQDVETIV 349

BLAST of CsGy3G015450 vs. NCBI nr
Match: XP_004147631.1 (diacylglycerol O-acyltransferase 3 [Cucumis sativus] >KGN57209.1 hypothetical protein Csa_011218 [Cucumis sativus])

HSP 1 Score: 623 bits (1606), Expect = 2.54e-224
Identity = 326/331 (98.49%), Postives = 326/331 (98.49%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG
Sbjct: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGNVKKEKEAVKKKLKLLKGLSA DYESSLLFRFDHGSIEE
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNVKKEKEAVKKKLKLLKGLSAADYESSLLFRFDHGSIEE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD
Sbjct: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNTTSYRLKKALSQP PDQYQANTIHVSTLPLPLQTQLLNSKTENIAVT STSVGR
Sbjct: 181 NEGHMNTTSYRLKKALSQPSPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTQSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI
Sbjct: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. NCBI nr
Match: ADN34049.1 (diacylglycerol acyltransferase [Cucumis melo subsp. melo])

HSP 1 Score: 566 bits (1459), Expect = 6.37e-202
Identity = 297/331 (89.73%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEVYG VS QLPGLF  DFSQSV V D DTRRGP +LS+SGRSVG ETRALGLV RRRM 
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGN+KKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSI+E
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIDE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIE+ REALTRQLQQLK EEKEQKRKKKLEKAKLKAART+ IHDS+SSSSESSD
Sbjct: 121 FQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNT SY+LKKALSQPFPDQY+ N IHVSTLPLPLQTQLLNSKTENI VT STSVGR
Sbjct: 181 NEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEF RVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDV RIVAEYLGQ+GGQTQSRLAPAAI
Sbjct: 301 GVGVEDVDRIVAEYLGQKGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. NCBI nr
Match: KAA0052150.1 (diacylglycerol acyltransferase [Cucumis melo var. makuwa] >TYK05115.1 diacylglycerol acyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 564 bits (1453), Expect = 5.22e-201
Identity = 296/331 (89.43%), Postives = 307/331 (92.75%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEV+G VS QLPGLF  DFSQSV V D DTRRGP +LS+ GRSVG ETRALGLV RRRM 
Sbjct: 1   MEVFGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSIYGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGN+KKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSI+E
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIDE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIE+ REALTRQLQQLK EEKEQKRKKKLEKAKLKAART+ IHDS+SSSSESSD
Sbjct: 121 FQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNT SYRLKKALSQPFPDQY+ N IHVSTLPLPLQTQLLNSKTENI VT STSVGR
Sbjct: 181 NEGHMNTRSYRLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEF RVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDV RIVAEYLGQ+GGQTQSRLAPAAI
Sbjct: 301 GVGVEDVDRIVAEYLGQKGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. NCBI nr
Match: XP_008439001.1 (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103483927 [Cucumis melo])

HSP 1 Score: 559 bits (1440), Expect = 4.99e-199
Identity = 293/331 (88.52%), Postives = 306/331 (92.45%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEVYG VS QLPGLF  DFSQSV V D DTRRGP +LS+SGRSVG ETRALGLV RRRM 
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGN+KKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSI+E
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIDE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIE+ REALTRQLQQLK EEK  ++KKKLEKAKLKAART+ IHDS+SSSSESSD
Sbjct: 121 FQSDRFSIEKTREALTRQLQQLKLEEKXAEKKKKLEKAKLKAARTKTIHDSSSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNT SY+LKKALSQPFPDQY+ N IHVSTLPLPLQTQLLNSKTENI VT STSVGR
Sbjct: 181 NEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEF RVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDV RIVAEYLGQ+GGQTQSRLAPAAI
Sbjct: 301 GVGVEDVDRIVAEYLGQKGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. NCBI nr
Match: XP_038900889.1 (diacylglycerol O-acyltransferase 3 [Benincasa hispida])

HSP 1 Score: 481 bits (1238), Expect = 2.74e-168
Identity = 258/329 (78.42%), Postives = 276/329 (83.89%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEV G VS  L   F  DF QS+ +S  D RR   +LS+SG SVGT TR LGL  R  M 
Sbjct: 1   MEVSGAVSRHLSAFFRHDFDQSIELSRADARRASCSLSISGGSVGTGTRTLGLASRWNMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDG+LRYYVGPTCHGG +KKEKEAVKKKLKLLKGLSATDYESSLLF  DHGSI E
Sbjct: 61  SSRFCDDGHLRYYVGPTCHGGRLKKEKEAVKKKLKLLKGLSATDYESSLLFHLDHGSIGE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQ+DRFSIEE REALTRQLQQLKSEEKEQKRKKKLEKAKLKAAR Q IHDS+ SSSESSD
Sbjct: 121 FQADRFSIEETREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARAQTIHDSSFSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NE HM   SYR++KALSQPFPDQYQ N I  STLP PLQTQLLNSK+ N+ V  STSVGR
Sbjct: 181 NEAHMINKSYRIRKALSQPFPDQYQTNAIQGSTLPPPLQTQLLNSKSGNVGV--STSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEFGRVMG + AVCGCKCMGKCRDGPNVRV G++++Q PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFGRVMGADGAVCGCKCMGKCRDGPNVRVSGALDIQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPA 329
           GVGVEDVGRIVAEYLGQE GQ QSR APA
Sbjct: 301 GVGVEDVGRIVAEYLGQERGQQQSRFAPA 327

BLAST of CsGy3G015450 vs. ExPASy TrEMBL
Match: A0A0A0L863 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171170 PE=4 SV=1)

HSP 1 Score: 623 bits (1606), Expect = 1.23e-224
Identity = 326/331 (98.49%), Postives = 326/331 (98.49%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG
Sbjct: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGNVKKEKEAVKKKLKLLKGLSA DYESSLLFRFDHGSIEE
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNVKKEKEAVKKKLKLLKGLSAADYESSLLFRFDHGSIEE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD
Sbjct: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNTTSYRLKKALSQP PDQYQANTIHVSTLPLPLQTQLLNSKTENIAVT STSVGR
Sbjct: 181 NEGHMNTTSYRLKKALSQPSPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTQSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI
Sbjct: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. ExPASy TrEMBL
Match: E5GC43 (Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 566 bits (1459), Expect = 3.08e-202
Identity = 297/331 (89.73%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEVYG VS QLPGLF  DFSQSV V D DTRRGP +LS+SGRSVG ETRALGLV RRRM 
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGN+KKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSI+E
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIDE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIE+ REALTRQLQQLK EEKEQKRKKKLEKAKLKAART+ IHDS+SSSSESSD
Sbjct: 121 FQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNT SY+LKKALSQPFPDQY+ N IHVSTLPLPLQTQLLNSKTENI VT STSVGR
Sbjct: 181 NEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEF RVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDV RIVAEYLGQ+GGQTQSRLAPAAI
Sbjct: 301 GVGVEDVDRIVAEYLGQKGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. ExPASy TrEMBL
Match: A0A5A7UAC7 (Diacylglycerol acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1769G00190 PE=4 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 2.53e-201
Identity = 296/331 (89.43%), Postives = 307/331 (92.75%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEV+G VS QLPGLF  DFSQSV V D DTRRGP +LS+ GRSVG ETRALGLV RRRM 
Sbjct: 1   MEVFGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSIYGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGN+KKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSI+E
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIDE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIE+ REALTRQLQQLK EEKEQKRKKKLEKAKLKAART+ IHDS+SSSSESSD
Sbjct: 121 FQSDRFSIEKTREALTRQLQQLKLEEKEQKRKKKLEKAKLKAARTKTIHDSSSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNT SYRLKKALSQPFPDQY+ N IHVSTLPLPLQTQLLNSKTENI VT STSVGR
Sbjct: 181 NEGHMNTRSYRLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEF RVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDV RIVAEYLGQ+GGQTQSRLAPAAI
Sbjct: 301 GVGVEDVDRIVAEYLGQKGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. ExPASy TrEMBL
Match: A0A1S3AXR9 (LOW QUALITY PROTEIN: uncharacterized protein LOC103483927 OS=Cucumis melo OX=3656 GN=LOC103483927 PE=4 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 2.42e-199
Identity = 293/331 (88.52%), Postives = 306/331 (92.45%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEVYG VS QLPGLF  DFSQSV V D DTRRGP +LS+SGRSVG ETRALGLV RRRM 
Sbjct: 1   MEVYGAVSRQLPGLFRRDFSQSVEVYDADTRRGPCSLSISGRSVGNETRALGLVSRRRMD 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIEE 120
           SSRFCDDGYLRYYVGPTC GGN+KKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSI+E
Sbjct: 61  SSRFCDDGYLRYYVGPTCQGGNLKKEKEAVKKKLKLLKGLSATDYESSLLFRFDHGSIDE 120

Query: 121 FQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSSESSD 180
           FQSDRFSIE+ REALTRQLQQLK EEK  ++KKKLEKAKLKAART+ IHDS+SSSSESSD
Sbjct: 121 FQSDRFSIEKTREALTRQLQQLKLEEKXAEKKKKLEKAKLKAARTKTIHDSSSSSSESSD 180

Query: 181 NEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHSTSVGR 240
           NEGHMNT SY+LKKALSQPFPDQY+ N IHVSTLPLPLQTQLLNSKTENI VT STSVGR
Sbjct: 181 NEGHMNTRSYQLKKALSQPFPDQYKTNAIHVSTLPLPLQTQLLNSKTENIGVTRSTSVGR 240

Query: 241 IQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQIPLCI 300
           I+VCMGNKCKKAGAAALMEEF RVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQ PLCI
Sbjct: 241 IEVCMGNKCKKAGAAALMEEFERVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQNPLCI 300

Query: 301 GVGVEDVGRIVAEYLGQEGGQTQSRLAPAAI 331
           GVGVEDV RIVAEYLGQ+GGQTQSRLAPAAI
Sbjct: 301 GVGVEDVDRIVAEYLGQKGGQTQSRLAPAAI 331

BLAST of CsGy3G015450 vs. ExPASy TrEMBL
Match: A0A6J1GV38 (diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662 GN=LOC111457454 PE=4 SV=1)

HSP 1 Score: 414 bits (1065), Expect = 3.00e-142
Identity = 228/333 (68.47%), Postives = 267/333 (80.18%), Query Frame = 0

Query: 1   MEVYGTVSGQLPGLFCLDFSQSVRVSDLDTRRGPSTLSVSGRSVGTETRALGLVGRRRMG 60
           MEV G V+ +L  LF  + +QSV V+  D RR P +LSVSG SV T T  +GLVGRR + 
Sbjct: 1   MEVSGAVTRRLSSLFRCEIAQSVDVASADARRAPCSLSVSGGSVSTGTHVIGLVGRRNID 60

Query: 61  SSRFCDDGYLRYYVGPTCHGGNVKKEKEAVKKKLKLLKGLS---ATDYESSLLFRFDHGS 120
           SSRF D+G+LRYYVGP C G  +KKEKEAVKKKLKLLKGLS   +TD+ES L+FRFD   
Sbjct: 61  SSRFSDNGHLRYYVGPNCQGRRLKKEKEAVKKKLKLLKGLSGYSSTDHESGLVFRFDDCL 120

Query: 121 IEEFQSDRFSIEERREALTRQLQQLKSEEKEQKRKKKLEKAKLKAARTQNIHDSTSSSS- 180
           I +FQ+DRFSIEE REAL +QLQQLKSEEK+QKR++KLEKAKLKA R + IH+S+SSSS 
Sbjct: 121 IGDFQADRFSIEETREALVKQLQQLKSEEKDQKRRRKLEKAKLKATRVKTIHESSSSSSS 180

Query: 181 ESSDNEGHMNTTSYRLKKALSQPFPDQYQANTIHVSTLPLPLQTQLLNSKTENIAVTHST 240
           ESSDNEG+M  TSY+++KALSQPFPDQ++ N I  STLP PLQ+QLLNS T+NI V  S 
Sbjct: 181 ESSDNEGYMTNTSYQIRKALSQPFPDQWKTNGIKESTLPPPLQSQLLNSDTKNIGVARSV 240

Query: 241 SVGRIQVCMGNKCKKAGAAALMEEFGRVMGDEAAVCGCKCMGKCRDGPNVRVLGSMEMQI 300
           SVGR++VCMGNKCKKAGAAALMEEF RVMG + AVCGCKCMGKCRDGPNVRV G++  Q 
Sbjct: 241 SVGRVEVCMGNKCKKAGAAALMEEFERVMGADGAVCGCKCMGKCRDGPNVRVSGALAGQS 300

Query: 301 ----PLCIGVGVEDVGRIVAEYLGQEGGQTQSR 325
               PLCIGVGVEDVGRIVA++LGQ+G   QSR
Sbjct: 301 CSANPLCIGVGVEDVGRIVADHLGQQGELKQSR 333

BLAST of CsGy3G015450 vs. TAIR 10
Match: AT1G48300.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 96 Blast hits to 87 proteins in 37 species: Archae - 0; Bacteria - 2; Metazoa - 12; Fungi - 2; Plants - 65; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 124.8 bits (312), Expect = 1.3e-28
Identity = 109/279 (39.07%), Postives = 149/279 (53.41%), Query Frame = 0

Query: 83  VKKEKEAVKKKLKLLKGLSAT-DYESSLLFRFD--HGSIEEFQSDRFSIEERREALTRQL 142
           ++KEK+A+KKK K+LK LS   D  SS+ F  D   G + E Q+   +I E  E L +QL
Sbjct: 1   MEKEKKALKKKAKVLKSLSKNLDMFSSIGFGLDPEAGLVGEIQTK--TISEATEILVKQL 60

Query: 143 QQLKSEEKEQKRKKKLEKAKLKAARTQNIHDS-TSSSSESSDNE---GHMNTTSYRLKKA 202
           +QLK+EEK  K+++K EKAK KA +     DS +SSSSESSD++   G +   S    KA
Sbjct: 61  EQLKAEEKILKKQRKEEKAKAKAMKKMTEMDSESSSSSESSDSDCDKGKVVDMSSLRNKA 120

Query: 203 LSQPFPDQYQANTIHVSTLP-----------------LPLQTQLLNSKTENIAVTHST-- 262
                P Q +A    V+TLP                 + LQT  +     N   T  T  
Sbjct: 121 KPVLEPLQPEAT---VATLPRIQEDAISCKNTSEALQIALQTSTIFPSMANPGQTLKTVE 180

Query: 263 -------SVGRIQVCMGNKCKKAGAAALMEEFGRVM-GDEAAVCGCKCMGKCRDGPNVRV 319
                   + R++VCMG KCK++G A L++EF R M G E +   CKCMGKCRDGPNVRV
Sbjct: 181 AVSVVGLPLNRVEVCMGGKCKRSGGALLLDEFQRAMTGFEGSAVACKCMGKCRDGPNVRV 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5W02.9e-3337.33Diacylglycerol O-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=DGAT3 PE=1... [more]
Match NameE-valueIdentityDescription
XP_004147631.12.54e-22498.49diacylglycerol O-acyltransferase 3 [Cucumis sativus] >KGN57209.1 hypothetical pr... [more]
ADN34049.16.37e-20289.73diacylglycerol acyltransferase [Cucumis melo subsp. melo][more]
KAA0052150.15.22e-20189.43diacylglycerol acyltransferase [Cucumis melo var. makuwa] >TYK05115.1 diacylglyc... [more]
XP_008439001.14.99e-19988.52PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103483927 [Cucumis me... [more]
XP_038900889.12.74e-16878.42diacylglycerol O-acyltransferase 3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0L8631.23e-22498.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G171170 PE=4 SV=1[more]
E5GC433.08e-20289.73Diacylglycerol acyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A5A7UAC72.53e-20189.43Diacylglycerol acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3AXR92.42e-19988.52LOW QUALITY PROTEIN: uncharacterized protein LOC103483927 OS=Cucumis melo OX=365... [more]
A0A6J1GV383.00e-14268.47diacylglycerol O-acyltransferase 3, cytosolic-like OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT1G48300.11.3e-2839.07unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 118..163
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 239..327
e-value: 1.4E-7
score: 33.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 165..186
NoneNo IPR availableCDDcd02980TRX_Fd_familycoord: 240..314
e-value: 3.07189E-9
score: 51.0888
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 240..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G015450.2CsGy3G015450.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity