CsGy3G013500 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G013500
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	3-dehydrosphinganine reductase TSC10A
Location	Gy14Chr3: 10089783 .. 10091918 (-)
RNA-Seq Expression	CsGy3G013500
Synteny	CsGy3G013500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGACCTCAATCTCGCTTTCATTTCTCTCGTTCTTCTTCTTCCTTTCGCGCTCCTCCTTTTCCTATACCTAATTGTCCGTCCACGTCCCGTTAGGATTTCTCTGAAGAGTCGCCATGTCTTTATTACCGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCCGCGACCGAGGGCGCGCGAGTCTCCATCCTAGCGCGTTCTCTCAAGAAGCTCGAGGAGGCAAAAGATGCTATTCGACTCTCCACTGGCATTGATGTGGCGGTCTACGCTGCCGATGTTCGAGATTACGATGCCATTTTGAAGGCTGTTGATGAAGCAGGTCCAATTGACGTTCTCATTGTGAATCAAGGCGTGTTTGTACCTCAGGAGCTGGCGGAGCAGGAATTGGATGAGGTCAAATTCATGTTAGATGTGAATTTATTGGGCACGTTTAACATGATTAAAGCTGCCTTACCTGCAATGAAGAATAGAGTGGACCGTCGACCTGCATCGATCGCTCTCATGTCTTCTCAGGCCGGTCAGGTAATTCATATAAAAGACGGAGAAATGAGCTTGGTTAGCATAAAGTACTTTGGAGTAGTTTTATGAAGTGTTTGTGTTCGAAACTTATACTCTAGAATGAGCTTCTTCGATTATTAGGGTGAATTCATTGACATTGGTGACATCCAGATACTCGCAAGCTGTAATTTCAATAGAATTGTAAATCTTTTACTCCTGAACGTCTTCTTATAAATCTGTGTAGGTGGGTATTTATGGTTACACGGCGTATTCAGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTTATTGGAGATGACATACATGTTTCTCTTATATTTCCTCCAGACACTGATACTCCTGGATTTGCAGAAGGTATGTGAAGAGCAAGTATCTGGTTTATGTTTACCTTGTAGTATTGTTTTGCTGGTCCTCAATTTGGGGTTTCAATACTTATCCGGTGAGTTGGAATATTCTTAGCTTAGTTTTCAGTTCATTGGTTTGTATTAAGATATGATGGGAAATGTCTTGGATGGATAAGCCGACTACAATATCACCTGGGTAGAATTTATTTATGTTAGAAATCATATTTATTGGATACCTCAGGCTAACAAAAATCCTTCAATTTATACCACAGAATCTTTTCTTAGTTGGTTTCTTCAATTATAAGTTGCTTATCCTTCATTGCAGAGCAAAAGAAAAGACCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCCGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTCATAATTCCCTGCAACTTTGAGGGAACATTATTGTCTATTGCAACTGCTGGCTTATCTCCTCAGAGATCATTCTTTATGGCGTTCATTGAAGTTGTTGGTGCTGGAGTAATAAGGATTGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATTGAAAAATGGCATGCTCAAAGAAACAGTAAGTTTTCTTTTAAATGATGGAACCATCAGCTTGTTTAGCCCATTATATATTTTCCGATAAAAGTAAAAAAATTAGTTCATTATTAATATCTGTGTTCTACAATTGCTTCGATCTTCTAAAAGCTCAATGTGCGGTTGGACTATTAGAAATGGCACTTTTTATCAGGCAGCAAAGAGATTTGTTTAAAATGGTCAAATTTTCACACATTCAAAAGCTATTAGATTTTTTATTTTGTTTTTTAACATAAGCATACAATTTTTGGTTCTTGCTTTCATTTTTTGTTAAGATTAAGATCTTCCTTCTCTTCTATTTTCTAACATATGATGATATGAATGACTCTGTCTTCCTTCTGAACTCTTGCTTCAACTGCTCTATTGTTAATGTCATATTAGATCAATATTCCAATTATCATATCTTTAACACATGTTCTCATACTGTTGGTGTCTGTTACGTCTTGTCTAAAGCTGATTCATGCTGTGAATTGACCAGTCACCTACCTAAGTAACAGGCAATCTATCAAATTGCATTCTTTGTTTGTTCCTTTCTCTCTGATTGTAAATTTAAGCCATTTTGATGACCGTGTTGTACTAGTAACCTTACCTATATTCTTTTTCTAGGAACCTAG

mRNA sequence

ATGGCGGACCTCAATCTCGCTTTCATTTCTCTCGTTCTTCTTCTTCCTTTCGCGCTCCTCCTTTTCCTATACCTAATTGTCCGTCCACGTCCCGTTAGGATTTCTCTGAAGAGTCGCCATGTCTTTATTACCGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCCGCGACCGAGGGCGCGCGAGTCTCCATCCTAGCGCGTTCTCTCAAGAAGCTCGAGGAGGCAAAAGATGCTATTCGACTCTCCACTGGCATTGATGTGGCGGTCTACGCTGCCGATGTTCGAGATTACGATGCCATTTTGAAGGCTGTTGATGAAGCAGGTCCAATTGACGTTCTCATTGTGAATCAAGGCGTGTTTGTACCTCAGGAGCTGGCGGAGCAGGAATTGGATGAGGTCAAATTCATGTTAGATGTGAATTTATTGGGCACGTTTAACATGATTAAAGCTGCCTTACCTGCAATGAAGAATAGAGTGGACCGTCGACCTGCATCGATCGCTCTCATGTCTTCTCAGGCCGGTCAGGTGGGTATTTATGGTTACACGGCGTATTCAGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTTATTGGAGATGACATACATGTTTCTCTTATATTTCCTCCAGACACTGATACTCCTGGATTTGCAGAAGAGCAAAAGAAAAGACCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCCGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTCATAATTCCCTGCAACTTTGAGGGAACATTATTGTCTATTGCAACTGCTGGCTTATCTCCTCAGAGATCATTCTTTATGGCGTTCATTGAAGTTGTTGGTGCTGGAGTAATAAGGATTGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATTGAAAAATGGCATGCTCAAAGAAACAGAACCTAG

Coding sequence (CDS)

ATGGCGGACCTCAATCTCGCTTTCATTTCTCTCGTTCTTCTTCTTCCTTTCGCGCTCCTCCTTTTCCTATACCTAATTGTCCGTCCACGTCCCGTTAGGATTTCTCTGAAGAGTCGCCATGTCTTTATTACCGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCCGCGACCGAGGGCGCGCGAGTCTCCATCCTAGCGCGTTCTCTCAAGAAGCTCGAGGAGGCAAAAGATGCTATTCGACTCTCCACTGGCATTGATGTGGCGGTCTACGCTGCCGATGTTCGAGATTACGATGCCATTTTGAAGGCTGTTGATGAAGCAGGTCCAATTGACGTTCTCATTGTGAATCAAGGCGTGTTTGTACCTCAGGAGCTGGCGGAGCAGGAATTGGATGAGGTCAAATTCATGTTAGATGTGAATTTATTGGGCACGTTTAACATGATTAAAGCTGCCTTACCTGCAATGAAGAATAGAGTGGACCGTCGACCTGCATCGATCGCTCTCATGTCTTCTCAGGCCGGTCAGGTGGGTATTTATGGTTACACGGCGTATTCAGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTTATTGGAGATGACATACATGTTTCTCTTATATTTCCTCCAGACACTGATACTCCTGGATTTGCAGAAGAGCAAAAGAAAAGACCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCCGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTCATAATTCCCTGCAACTTTGAGGGAACATTATTGTCTATTGCAACTGCTGGCTTATCTCCTCAGAGATCATTCTTTATGGCGTTCATTGAAGTTGTTGGTGCTGGAGTAATAAGGATTGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATTGAAAAATGGCATGCTCAAAGAAACAGAACCTAG

Protein sequence

MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGVIRIVALCFQWVWYGSIEKWHAQRNRT*

Homology

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BLAST of CsGy3G013500 vs. ExPASy Swiss-Prot
Match: Q0WRJ2 (3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana OX=3702 GN=TSC10A PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.3e-121
Identity = 219/320 (68.44%), Postives = 272/320 (85.00%), Query Frame = 0


Query: 6   LAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGARV 65
           L  + L+ ++P +LL  L LIVRPRP++I +KSRH FITGGSSGIGLALAH+AA+EGARV
Sbjct: 7   LFLLFLIPIIPLSLLAILALIVRPRPIKIPIKSRHAFITGGSSGIGLALAHRAASEGARV 66

Query: 66  SILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQGVFVP 125
           SILARS  KLEEAK +I+L+TG++VA ++ADVRDYDA+ KA+DE+GPIDVLIVNQGVF  
Sbjct: 67  SILARSGSKLEEAKKSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFTA 126

Query: 126 QELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYT 185
           +EL +   ++VKF +DVNL+G+FN+IKAALPAMK R DR PASI+L+SSQAGQVG+YGY 
Sbjct: 127 KELVKHSPEDVKFTIDVNLVGSFNVIKAALPAMKARKDRGPASISLVSSQAGQVGVYGYA 186

Query: 186 AYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIAAGGGS 245
           AYSASKFG++GLA+ALQQEVI DDIHV+LIFPPDT+TPGF EEQK RPE+T IIAA  GS
Sbjct: 187 AYSASKFGLQGLAQALQQEVISDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAIIAASSGS 246

Query: 246 MKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGVIRIVA 305
           M+ E VAKKA +GIK+G+F + CNFEG LLS+AT G+SPQRSF++AF+EV+ AG IR++A
Sbjct: 247 METEEVAKKAMDGIKAGNFTVSCNFEGFLLSLATTGMSPQRSFWLAFLEVITAGPIRLIA 306

Query: 306 LCFQWVWYGSIEKWHAQRNR 326
           L FQW WY +IEKW   + +
Sbjct: 307 LFFQWDWYKAIEKWSKTKTK 326

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BLAST of CsGy3G013500 vs. ExPASy Swiss-Prot
Match: F4JZN6 (3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana OX=3702 GN=TSC10B PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.4e-112
Identity = 214/325 (65.85%), Postives = 266/325 (81.85%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MA +   F+  +L +  +LL+ L  IVRPR V I +K RHVFITGGSSGIGLALAH+A +
Sbjct: 1   MAAIFSLFLFFILFI-VSLLIILSFIVRPRSVTIPIKFRHVFITGGSSGIGLALAHRAVS 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGA+VSILARS +KL EAK +I+L+TG++VA ++ADVRDYDA+ KA+DE+GPIDVLIVNQ
Sbjct: 61  EGAKVSILARSTEKLAEAKRSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVF+ +EL +Q  +EVKFM+DVNL G+FN+IKAALPAMK R  R PASI+L+SSQAGQ G
Sbjct: 121 GVFIGKELEKQSPEEVKFMIDVNLTGSFNVIKAALPAMKAREGRGPASISLVSSQAGQAG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFG++GLA+ALQQEVI D IHV+L+FPPDTDTPGF +E KKRPELT+IIA
Sbjct: 181 IYGYTAYSASKFGLQGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           A  GSMK   VAK  F+GIK+G F + C+F G LLSIA+ G+SPQ SF++A  EV+  G+
Sbjct: 241 ASSGSMKTNEVAKICFDGIKAGKFTVTCHFIGFLLSIASTGMSPQGSFWLALTEVMFGGL 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRNR 326
           IR+ +L FQW WY +IEKW +QRN+
Sbjct: 301 IRLASLVFQWQWYKTIEKW-SQRNK 323

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BLAST of CsGy3G013500 vs. ExPASy Swiss-Prot
Match: Q06136 (3-ketodihydrosphingosine reductase OS=Homo sapiens OX=9606 GN=KDSR PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.6e-44
Identity = 117/330 (35.45%), Postives = 192/330 (58.18%), Query Frame = 0


Query: 6   LAFISLVLLLPFALLLFLYL-IVRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGAR 65
           +  ++   L+ F LLL++   ++ P+P  ++L   HV +TGGSSGIG  +A +   +GA 
Sbjct: 1   MLLLAAAFLVAFVLLLYMVSPLISPKP--LALPGAHVVVTGGSSGIGKCIAIECYKQGAF 60

Query: 66  VSILARSLKKLEEAKDAIRLSTGID---VAVYAADV-RDY----DAILKAVDEAGPIDVL 125
           ++++AR+  KL +AK  I + +  D   V   + DV +DY    + I +A ++ GP+D+L
Sbjct: 61  ITLVARNEDKLLQAKKEIEMHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDML 120

Query: 126 IVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQA 185
           +   G+ V  +  + E+   + ++ +N LG+    +A +  MK   +RR   I  +SSQA
Sbjct: 121 VNCAGMAVSGKFEDLEVSTFERLMSINYLGSVYPSRAVITTMK---ERRVGRIVFVSSQA 180

Query: 186 GQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELT 245
           GQ+G++G+TAYSASKF IRGLAEALQ EV   ++++++ +PPDTDTPGFAEE + +P  T
Sbjct: 181 GQLGLFGFTAYSASKFAIRGLAEALQMEVKPYNVYITVAYPPDTDTPGFAEENRTKPLET 240

Query: 246 TIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVV 305
            +I+      KPE VAK+       G+F      +G +LS  T G++P  S      +VV
Sbjct: 241 RLISETTSVCKPEQVAKQIVKDAIQGNFNSSLGSDGYMLSALTCGMAPVTSITEGLQQVV 300

Query: 306 GAGVIRIVALCFQWVWYGSIEKWHAQRNRT 327
             G+ R +AL +   +   + +   QR ++
Sbjct: 301 TMGLFRTIALFYLGSFDSIVRRCMMQREKS 325

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BLAST of CsGy3G013500 vs. ExPASy Swiss-Prot
Match: Q6GV12 (3-ketodihydrosphingosine reductase OS=Mus musculus OX=10090 GN=Kdsr PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.2e-43
Identity = 115/304 (37.83%), Postives = 179/304 (58.88%), Query Frame = 0


Query: 14  LLPFALLLFLYL-IVRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGARVSILARSL 73
           L+ F LLL++   ++ P+P  ++L   HV +TGGSSGIG  +A +   +GA ++++AR+ 
Sbjct: 9   LVAFVLLLYMVSPLISPKP--LALPGAHVVVTGGSSGIGKCIAIECYKQGAFITLVARNE 68

Query: 74  KKLEEAKDAIRLSTGID---VAVYAADV-RDY----DAILKAVDEAGPIDVLIVNQGVFV 133
            KL +AK  I   +  D   V   + DV +DY    + I +A ++ GP+D+L+   G  +
Sbjct: 69  DKLLQAKKDIEKHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNCAGTSM 128

Query: 134 PQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGY 193
             +  E E+   + ++ +N LG+    +A +  MK   +RR   I  +SSQAGQ+G++G+
Sbjct: 129 SGKFEELEVSSFEKLMSINYLGSVYPSRAVITTMK---ERRVGRIVFVSSQAGQLGLFGF 188

Query: 194 TAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIAAGGG 253
           TAYS+SKF IRGLAEALQ EV   +++V++ +PPDTDTPG AEE K +P  T +I+    
Sbjct: 189 TAYSSSKFAIRGLAEALQMEVKPYNVYVTVAYPPDTDTPGLAEENKTKPLETRLISETTA 248

Query: 254 SMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGVIRIV 309
             KPE VAK+       G+F      +G +LS  T G++P  S      +VV  G+ R +
Sbjct: 249 ICKPEQVAKQIVKDAIQGNFNSSIGSDGYMLSSLTCGMAPVTSITEGLQQVVTMGLFRTI 307

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BLAST of CsGy3G013500 vs. ExPASy Swiss-Prot
Match: Q2KIJ5 (3-ketodihydrosphingosine reductase OS=Bos taurus OX=9913 GN=KDSR PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-41
Identity = 112/312 (35.90%), Postives = 186/312 (59.62%), Query Frame = 0


Query: 6   LAFISLVLLLPFALLLFLYL-IVRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGAR 65
           +  ++   L+ F LLL++   ++ P+P  ++L   HV +TGGSSGIG  +A +   +GA 
Sbjct: 1   MLLLAAASLVAFVLLLYMVSPLISPKP--LALPGAHVVVTGGSSGIGKCIAIECYKQGAF 60

Query: 66  VSILARSLKKLEEAKDAIRLSTGID---VAVYAADV-RDY----DAILKAVDEAGPIDVL 125
           ++++AR+  KL +AK  I   +  D   V   + DV +DY    + I +A ++ GP+D+L
Sbjct: 61  ITLVARNEDKLLQAKKEIEKHSINDKQVVLCISVDVSQDYSQVENVIKQAQEKLGPVDML 120

Query: 126 IVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQA 185
           +   G+ +  +  + E+   + ++ +N LG+    +A +  MK   +RR   +  +SSQA
Sbjct: 121 VNCAGMSLSGKFEDLEVSTFERLMSINYLGSVYPSRAVIATMK---ERRMGRVVFVSSQA 180

Query: 186 GQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELT 245
           GQ+G++GYTAYS+SKF +RGLAEALQ EV   +++V++ +PPDTDTPGFA+E + +P  T
Sbjct: 181 GQLGLFGYTAYSSSKFALRGLAEALQMEVKPYNVYVTVAYPPDTDTPGFAKENQTKPLET 240

Query: 246 TIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVV 305
            +I+      KPE VAK+    ++ G+F      +G +LS  T G++P  S      +VV
Sbjct: 241 RLISETTSVCKPEQVAKQIVKDVQ-GNFNSSIGSDGYMLSSLTCGMAPVTSIMEGLQQVV 300

Query: 306 GAGVIRIVALCF 309
             G+ R +AL +
Sbjct: 301 TMGLFRTIALFY 306

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BLAST of CsGy3G013500 vs. NCBI nr
Match: XP_004134147.1 (3-dehydrosphinganine reductase TSC10A [Cucumis sativus] >KGN57010.1 hypothetical protein Csa_009962 [Cucumis sativus])

HSP 1 Score: 617 bits (1591), Expect = 3.33e-222
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326
           IRIVALCFQWVWYGSIEKWHAQRNRT
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326

[Close]

BLAST of CsGy3G013500 vs. NCBI nr
Match: XP_008438707.1 (PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo] >KAA0049383.1 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo var. makuwa] >TYK17176.1 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 597 bits (1540), Expect = 1.83e-214
Identity = 313/324 (96.60%), Postives = 318/324 (98.15%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLN AFISL+LLLP ALLLFLYLIVRPRPV+I LKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNFAFISLILLLPLALLLFLYLIVRPRPVKIPLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFFMAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFFMAFLEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRN 324
           IRIVALCFQWVWYGSIEKWHAQR 
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of CsGy3G013500 vs. NCBI nr
Match: XP_016899126.1 (PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X1 [Cucumis melo])

HSP 1 Score: 597 bits (1540), Expect = 1.90e-214
Identity = 313/324 (96.60%), Postives = 318/324 (98.15%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLN AFISL+LLLP ALLLFLYLIVRPRPV+I LKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNFAFISLILLLPLALLLFLYLIVRPRPVKIPLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFFMAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFFMAFLEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRN 324
           IRIVALCFQWVWYGSIEKWHAQR 
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of CsGy3G013500 vs. NCBI nr
Match: XP_038892516.1 (3-dehydrosphinganine reductase TSC10A-like isoform X2 [Benincasa hispida])

HSP 1 Score: 586 bits (1511), Expect = 5.18e-210
Identity = 307/326 (94.17%), Postives = 316/326 (96.93%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MAD N AFISL+LLLPFALL+ LYLIVRPRPV+I LK+RHVFITGGSSGIGLALAHQAA+
Sbjct: 1   MADFNYAFISLLLLLPFALLVLLYLIVRPRPVKIPLKNRHVFITGGSSGIGLALAHQAAS 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           E ARVSILARS KKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAV EAGPIDVLIVNQ
Sbjct: 61  EAARVSILARSHKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVHEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKK+FNGIKSGSFIIPCNFEG LLSIATAGLSPQRSF MAF+EVVGAG+
Sbjct: 241 AGGGSMKPELVAKKSFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGI 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326
           IRIVALCFQWVWYGSIEKWHAQRNRT
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326

[Close]

BLAST of CsGy3G013500 vs. NCBI nr
Match: XP_038892507.1 (3-dehydrosphinganine reductase TSC10A-like isoform X1 [Benincasa hispida])

HSP 1 Score: 582 bits (1500), Expect = 4.96e-208
Identity = 305/324 (94.14%), Postives = 314/324 (96.91%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MAD N AFISL+LLLPFALL+ LYLIVRPRPV+I LK+RHVFITGGSSGIGLALAHQAA+
Sbjct: 1   MADFNYAFISLLLLLPFALLVLLYLIVRPRPVKIPLKNRHVFITGGSSGIGLALAHQAAS 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           E ARVSILARS KKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAV EAGPIDVLIVNQ
Sbjct: 61  EAARVSILARSHKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVHEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKK+FNGIKSGSFIIPCNFEG LLSIATAGLSPQRSF MAF+EVVGAG+
Sbjct: 241 AGGGSMKPELVAKKSFNGIKSGSFIIPCNFEGALLSIATAGLSPQRSFLMAFLEVVGAGI 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRN 324
           IRIVALCFQWVWYGSIEKWHAQRN
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRN 324

[Close]

BLAST of CsGy3G013500 vs. ExPASy TrEMBL
Match: A0A0A0L506 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149900 PE=4 SV=1)

HSP 1 Score: 617 bits (1591), Expect = 1.61e-222
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326
           IRIVALCFQWVWYGSIEKWHAQRNRT
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326

[Close]

BLAST of CsGy3G013500 vs. ExPASy TrEMBL
Match: A0A5D3D2X9 (3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00200 PE=4 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 8.87e-215
Identity = 313/324 (96.60%), Postives = 318/324 (98.15%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLN AFISL+LLLP ALLLFLYLIVRPRPV+I LKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNFAFISLILLLPLALLLFLYLIVRPRPVKIPLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFFMAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFFMAFLEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRN 324
           IRIVALCFQWVWYGSIEKWHAQR 
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of CsGy3G013500 vs. ExPASy TrEMBL
Match: A0A1S3AX60 (3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483732 PE=4 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 8.87e-215
Identity = 313/324 (96.60%), Postives = 318/324 (98.15%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLN AFISL+LLLP ALLLFLYLIVRPRPV+I LKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNFAFISLILLLPLALLLFLYLIVRPRPVKIPLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFFMAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFFMAFLEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRN 324
           IRIVALCFQWVWYGSIEKWHAQR 
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of CsGy3G013500 vs. ExPASy TrEMBL
Match: A0A1S4DT08 (3-dehydrosphinganine reductase TSC10A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483732 PE=4 SV=1)

HSP 1 Score: 597 bits (1540), Expect = 9.21e-215
Identity = 313/324 (96.60%), Postives = 318/324 (98.15%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MADLN AFISL+LLLP ALLLFLYLIVRPRPV+I LKSRHVFITGGSSGIGLALAHQAAT
Sbjct: 1   MADLNFAFISLILLLPLALLLFLYLIVRPRPVKIPLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAISKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTTIIA
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFFMAF+EVVGAGV
Sbjct: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFFMAFLEVVGAGV 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRN 324
           IRIVALCFQWVWYGSIEKWHAQR 
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRK 324

[Close]

BLAST of CsGy3G013500 vs. ExPASy TrEMBL
Match: A0A6J1IDV1 (3-dehydrosphinganine reductase TSC10A OS=Cucurbita maxima OX=3661 GN=LOC111472258 PE=4 SV=1)

HSP 1 Score: 552 bits (1423), Expect = 6.40e-197
Identity = 285/326 (87.42%), Postives = 305/326 (93.56%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MAD N AFISLVLLLP ALL+FLYLIVRPR V I LK+RHVFITGGSSGIGLALAHQAA+
Sbjct: 1   MADFNYAFISLVLLLPLALLIFLYLIVRPRSVNIPLKNRHVFITGGSSGIGLALAHQAAS 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGARVSILARSLKKLEEAKDA+RLSTGIDVAV+AADVRDYDAI KAVDEAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAVRLSTGIDVAVFAADVRDYDAISKAVDEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVFVPQELAEQ++DEVKFMLDVNLLG F+MIKAALP MKNRVDRRPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQDMDEVKFMLDVNLLGAFHMIKAALPTMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           +YGYTAYSASKFG+RGLAEALQQEVIGD+IH+SLIFPPDTDTPG  EE+K+RPELT IIA
Sbjct: 181 VYGYTAYSASKFGLRGLAEALQQEVIGDNIHISLIFPPDTDTPGLVEEEKRRPELTNIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           AGGG MK ELVAKKA NGIKSGSFIIPCNFEG LLS+ATAGLSPQRSF MAF+EVV AG+
Sbjct: 241 AGGGRMKAELVAKKALNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFLMAFLEVVTAGI 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326
           IRIV LCFQWVWY SI+KWHAQ+ RT
Sbjct: 301 IRIVGLCFQWVWYDSIQKWHAQKKRT 326

[Close]

BLAST of CsGy3G013500 vs. TAIR 10
Match: AT3G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 435.3 bits (1118), Expect = 4.5e-122
Identity = 219/320 (68.44%), Postives = 272/320 (85.00%), Query Frame = 0


Query: 6   LAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGARV 65
           L  + L+ ++P +LL  L LIVRPRP++I +KSRH FITGGSSGIGLALAH+AA+EGARV
Sbjct: 7   LFLLFLIPIIPLSLLAILALIVRPRPIKIPIKSRHAFITGGSSGIGLALAHRAASEGARV 66

Query: 66  SILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQGVFVP 125
           SILARS  KLEEAK +I+L+TG++VA ++ADVRDYDA+ KA+DE+GPIDVLIVNQGVF  
Sbjct: 67  SILARSGSKLEEAKKSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFTA 126

Query: 126 QELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYT 185
           +EL +   ++VKF +DVNL+G+FN+IKAALPAMK R DR PASI+L+SSQAGQVG+YGY 
Sbjct: 127 KELVKHSPEDVKFTIDVNLVGSFNVIKAALPAMKARKDRGPASISLVSSQAGQVGVYGYA 186

Query: 186 AYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIAAGGGS 245
           AYSASKFG++GLA+ALQQEVI DDIHV+LIFPPDT+TPGF EEQK RPE+T IIAA  GS
Sbjct: 187 AYSASKFGLQGLAQALQQEVISDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAIIAASSGS 246

Query: 246 MKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGVIRIVA 305
           M+ E VAKKA +GIK+G+F + CNFEG LLS+AT G+SPQRSF++AF+EV+ AG IR++A
Sbjct: 247 METEEVAKKAMDGIKAGNFTVSCNFEGFLLSLATTGMSPQRSFWLAFLEVITAGPIRLIA 306

Query: 306 LCFQWVWYGSIEKWHAQRNR 326
           L FQW WY +IEKW   + +
Sbjct: 307 LFFQWDWYKAIEKWSKTKTK 326

[Close]

BLAST of CsGy3G013500 vs. TAIR 10
Match: AT5G19200.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 406.8 bits (1044), Expect = 1.7e-113
Identity = 214/325 (65.85%), Postives = 266/325 (81.85%), Query Frame = 0


Query: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60
           MA +   F+  +L +  +LL+ L  IVRPR V I +K RHVFITGGSSGIGLALAH+A +
Sbjct: 1   MAAIFSLFLFFILFI-VSLLIILSFIVRPRSVTIPIKFRHVFITGGSSGIGLALAHRAVS 60

Query: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120
           EGA+VSILARS +KL EAK +I+L+TG++VA ++ADVRDYDA+ KA+DE+GPIDVLIVNQ
Sbjct: 61  EGAKVSILARSTEKLAEAKRSIQLATGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180
           GVF+ +EL +Q  +EVKFM+DVNL G+FN+IKAALPAMK R  R PASI+L+SSQAGQ G
Sbjct: 121 GVFIGKELEKQSPEEVKFMIDVNLTGSFNVIKAALPAMKAREGRGPASISLVSSQAGQAG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTTIIA 240
           IYGYTAYSASKFG++GLA+ALQQEVI D IHV+L+FPPDTDTPGF +E KKRPELT+IIA
Sbjct: 181 IYGYTAYSASKFGLQGLAQALQQEVISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIA 240

Query: 241 AGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300
           A  GSMK   VAK  F+GIK+G F + C+F G LLSIA+ G+SPQ SF++A  EV+  G+
Sbjct: 241 ASSGSMKTNEVAKICFDGIKAGKFTVTCHFIGFLLSIASTGMSPQGSFWLALTEVMFGGL 300

Query: 301 IRIVALCFQWVWYGSIEKWHAQRNR 326
           IR+ +L FQW WY +IEKW +QRN+
Sbjct: 301 IRLASLVFQWQWYKTIEKW-SQRNK 323

[Close]

BLAST of CsGy3G013500 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 3.1e-14
Identity = 67/223 (30.04%), Postives = 106/223 (47.53%), Query Frame = 0


Query: 36  LKSRHVFITGGSSGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAA 95
           L+ +   ITGG+SGIG A A +  ++GA+V I+       EEA   +    G        
Sbjct: 36  LEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDID----EEAGHMVATELGSAAHFLRC 95

Query: 96  DVRDYDAILKAVDEA----GPIDVLIVNQGV---FVPQELAEQELDEVKFMLDVNLLGTF 155
           DV + + I KAV+ A    G +DV++ + G+     P  +A+ ++D    ++ +N+ GT 
Sbjct: 96  DVTEEEQIAKAVETAVTRHGKLDVMLNSAGISCSISPPSIADLDMDTYDKVMRLNVRGTV 155

Query: 156 NMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGD 215
             IK A  AM   +     SI  +SS +G +G  G  AYS SKF I G+ + +  E+   
Sbjct: 156 LGIKHAARAM---IPAGSGSILCLSSISGLMGGLGPHAYSISKFTIPGVVKTVASELCKH 215

Query: 216 DIHVSLIFPPDTDTP--------GFAEEQKKRPELTTIIAAGG 244
            + ++ I P    TP         FA    +  +L  I+ A G
Sbjct: 216 GLRINCISPAGIPTPLTLRMFREAFAGHSIREEQLLAIVNATG 251

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BLAST of CsGy3G013500 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.2e-13
Identity = 68/237 (28.69%), Postives = 109/237 (45.99%), Query Frame = 0


Query: 27  VRPRPVRISLKSRHVFITGGSSGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLST 86
           +R +P + SL+     +TGGS G+G A+  + A  GARV   AR   +L+E+    + + 
Sbjct: 8   LRDKP-KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQ-AK 67

Query: 87  GIDVAVYAADVRDYDAILKAVDEA-----GPIDVLIVNQGVFVPQELAEQELDEVKFMLD 146
           G+ V     DV   D   K ++       G + +L+ N G+ V +   E   +E  F++ 
Sbjct: 68  GLQVTTSVCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIA 127

Query: 147 VNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEAL 206
            NL  TF+  + A P +K        +I LMSS AG V +   + Y A+K  +  LA  L
Sbjct: 128 TNLESTFHFSQLAHPLLK---ASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNL 187

Query: 207 QQEVIGDDIHVSLIFPPDTDTP------GFAEEQKKRPELTTIIAAGGGSMKPELVA 253
             E   D+I  + + P    TP      G  + ++K   +T +   G  +    LVA
Sbjct: 188 ACEWASDNIRANSVCPWFITTPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVA 239

[Close]

BLAST of CsGy3G013500 vs. TAIR 10
Match: AT5G50600.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-13
Identity = 60/200 (30.00%), Postives = 98/200 (49.00%), Query Frame = 0


Query: 35  SLKSRHVFITGGSSGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYA 94
           +L  + V ITG SSGIG  LA++ A  GA +++ AR   +LEE  +  R     +V    
Sbjct: 44  NLYGKVVLITGASSGIGEQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVH 103

Query: 95  ADVRDYDAILKAVDEA----GPIDVLIVNQGVFVPQELAE-QELDEVKFMLDVNLLGTFN 154
           ADV   D   + VD+     G +D L+ N G+         +++   K +LD N  G+  
Sbjct: 104 ADVSKPDDCRRIVDDTITHFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVY 163

Query: 155 MIKAALPAMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDD 214
             +AALP ++    +    I  MSS A  +     + Y+ASK  +    E ++ E +G D
Sbjct: 164 TTRAALPYLR----QSNGKIVAMSSSAAWLTAPRMSFYNASKAALLSFFETMRIE-LGGD 223

Query: 215 IHVSLIFPPDTDTPGFAEEQ 230
           +H++++      TPG+ E +
Sbjct: 224 VHITIV------TPGYIESE 232

The following BLAST results are available for this feature:

BLAST of CsGy3G013500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WRJ2	6.3e-121	68.44	3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana OX=3702 GN=TSC10A ...	[more]
F4JZN6	2.4e-112	65.85	3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana OX=3702 GN=TSC10B ...	[more]
Q06136	1.6e-44	35.45	3-ketodihydrosphingosine reductase OS=Homo sapiens OX=9606 GN=KDSR PE=1 SV=1	[more]
Q6GV12	5.2e-43	37.83	3-ketodihydrosphingosine reductase OS=Mus musculus OX=10090 GN=Kdsr PE=1 SV=1	[more]
Q2KIJ5	1.3e-41	35.90	3-ketodihydrosphingosine reductase OS=Bos taurus OX=9913 GN=KDSR PE=2 SV=1	[more]

BLAST of CsGy3G013500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134147.1	3.33e-222	100.00	3-dehydrosphinganine reductase TSC10A [Cucumis sativus] >KGN57010.1 hypothetical...	[more]
XP_008438707.1	1.83e-214	96.60	PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X2 [Cucumis melo] >KAA0...	[more]
XP_016899126.1	1.90e-214	96.60	PREDICTED: 3-dehydrosphinganine reductase TSC10A isoform X1 [Cucumis melo]	[more]
XP_038892516.1	5.18e-210	94.17	3-dehydrosphinganine reductase TSC10A-like isoform X2 [Benincasa hispida]	[more]
XP_038892507.1	4.96e-208	94.14	3-dehydrosphinganine reductase TSC10A-like isoform X1 [Benincasa hispida]	[more]

BLAST of CsGy3G013500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L506	1.61e-222	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149900 PE=4 SV=1	[more]
A0A5D3D2X9	8.87e-215	96.60	3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3AX60	8.87e-215	96.60	3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S4DT08	9.21e-215	96.60	3-dehydrosphinganine reductase TSC10A isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1IDV1	6.40e-197	87.42	3-dehydrosphinganine reductase TSC10A OS=Cucurbita maxima OX=3661 GN=LOC11147225...	[more]

BLAST of CsGy3G013500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06060.1	4.5e-122	68.44	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G19200.1	1.7e-113	65.85	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26760.1	3.1e-14	30.04	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	1.2e-13	28.69	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50600.1	2.0e-13	30.00	hydroxysteroid dehydrogenase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 187..206 score: 34.31 coord: 40..57 score: 44.59 coord: 161..177 score: 23.5 coord: 111..122 score: 45.43
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 40..231 e-value: 4.3E-45 score: 153.6
None	No IPR available	GENE3D	3.40.50.720		coord: 33..274 e-value: 2.5E-58 score: 199.6
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 43..221 e-value: 1.7E-30 score: 104.0
None	No IPR available	PANTHER	PTHR43550:SF7	3-DEHYDROSPHINGANINE REDUCTASE TSC10A-LIKE ISOFORM X1	coord: 10..325
None	No IPR available	PANTHER	PTHR43550	3-KETODIHYDROSPHINGOSINE REDUCTASE	coord: 10..325
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 174..202
IPR045022	3-ketodihydrosphingosine reductase KDSR-like	CDD	cd08939	KDSR-like_SDR_c	coord: 39..272 e-value: 4.23153E-90 score: 267.197
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 34..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G013500.2	CsGy3G013500.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006666	3-keto-sphinganine metabolic process
biological_process	GO:0030148	sphingolipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0047560	3-dehydrosphinganine reductase activity
molecular_function	GO:0016491	oxidoreductase activity