CsGy2G025350 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G025350
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionEF hand calcium-binding protein family
LocationGy14Chr2: 32809977 .. 32810762 (-)
RNA-Seq ExpressionCsGy2G025350
SyntenyCsGy2G025350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCGCTGAATTTGGGCTTTTTCCACGTTTTCTAGACTCTTCAATTCATTATTTAATCCCACCAACATTTTTCACACACAATTCTTTATTCCACAATCCACTTCGTTGAATCCGTGTAACAAAAGAAAAAAAGTAATGGTCGACCATCCTCTCCACCAGTTGACGGCAACTACCATTCTCATTTACCAATTCCGACGGTCAAACCACGGAACCAGCACGCGCATTCTCTTTCTTATAATTTCACACCAGATGTTTCCCCAATCCCCCAATTCTTCAACTTCAATTCTCTCTTACTTTACCCTTCACTCGAATTCAAAATCATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAAGTCGACGGCGACGGCGATGGGAAACTCTCTCCGCCGGAGCTGCAGCGGTGTATATTGGGAGTTGGGGGCAGTTTGACGATCGAGGAGGCGGAGACGGTGGTAGAGAAATTGGATTCGGACGGTGATGGGTTGGTGGGATGGGATGAGTTTGTTGAATTTGTGGAAGGAGTTGGAGAGGAAGAGAAAGTGAATGATTTGAAAGAAGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTTATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGGAGTATTGATGATTGCAAGAAAATGATTGCCAAATTTGATCTTGATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCTTGAACTTTCTTCCATTGTAGCTTCTCAACCTTTTTTTTCCTTTTTG

mRNA sequence

CTTCGCTGAATTTGGGCTTTTTCCACGTTTTCTAGACTCTTCAATTCATTATTTAATCCCACCAACATTTTTCACACACAATTCTTTATTCCACAATCCACTTCGTTGAATCCGTGTAACAAAAGAAAAAAAGTAATGGTCGACCATCCTCTCCACCAGTTGACGGCAACTACCATTCTCATTTACCAATTCCGACGGTCAAACCACGGAACCAGCACGCGCATTCTCTTTCTTATAATTTCACACCAGATGTTTCCCCAATCCCCCAATTCTTCAACTTCAATTCTCTCTTACTTTACCCTTCACTCGAATTCAAAATCATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAAGTCGACGGCGACGGCGATGGGAAACTCTCTCCGCCGGAGCTGCAGCGGTGTATATTGGGAGTTGGGGGCAGTTTGACGATCGAGGAGGCGGAGACGGTGGTAGAGAAATTGGATTCGGACGGTGATGGGTTGGTGGGATGGGATGAGTTTGTTGAATTTGTGGAAGGAGTTGGAGAGGAAGAGAAAGTGAATGATTTGAAAGAAGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTTATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGGAGTATTGATGATTGCAAGAAAATGATTGCCAAATTTGATCTTGATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCTTGAACTTTCTTCCATTGTAGCTTCTCAACCTTTTTTTTCCTTTTTG

Coding sequence (CDS)

ATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAAGTCGACGGCGACGGCGATGGGAAACTCTCTCCGCCGGAGCTGCAGCGGTGTATATTGGGAGTTGGGGGCAGTTTGACGATCGAGGAGGCGGAGACGGTGGTAGAGAAATTGGATTCGGACGGTGATGGGTTGGTGGGATGGGATGAGTTTGTTGAATTTGTGGAAGGAGTTGGAGAGGAAGAGAAAGTGAATGATTTGAAAGAAGCGTTTAGGATGTATGAAATGGATGGTTGTGGGTTTATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGGAGTATTGATGATTGCAAGAAAATGATTGCCAAATTTGATCTTGATTCTGATGGTGTTCTTAATTTTGATGAGTTTAAGTTCATGATGTCTTGA

Protein sequence

MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKFDLDSDGVLNFDEFKFMMS*
Homology
BLAST of CsGy2G025350 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.9e-30
Identity = 68/138 (49.28%), Postives = 94/138 (68.12%), Query Frame = 0

Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+  + +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAK 121
           EF + ++   EEEK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ DDC+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLDSDGVLNFDEFKFMM 140
           FDL++DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

BLAST of CsGy2G025350 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.4e-29
Identity = 69/139 (49.64%), Postives = 93/139 (66.91%), Query Frame = 0

Query: 5   ALYEKVFKQVDGDGDGKLSPPELQRCI-LGVGGSLTIEEAETVVEKLDSDGDGLVGWDEF 64
           A + +VF   D D DGK+S  EL+ C+   +G  ++ EEAE +V   D+D DGL+  +EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  VEF---VEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 124
            +    +E   EEE+   L EAFRMYEM+G G IT  SLKRMLSKLG  + I++C+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDLDSDGVLNFDEFKFMM 140
           +FDLD DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

BLAST of CsGy2G025350 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 5.4e-29
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 10  VFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVEG 69
           VF  +D + DGK+S  ELQ C+  +GG+L+  E E VV+  D DGDG + ++EF++ +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKFDLDSD 129
               +EE+  +LKEAF MY M+G  FIT  SL+R LS+LGES ++D CK MI  FD + D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

BLAST of CsGy2G025350 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 5.1e-27
Identity = 64/138 (46.38%), Postives = 91/138 (65.94%), Query Frame = 0

Query: 8   EKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVE-F 67
           +KVF++ D +GDGK+S  EL+  I  +  + + EE  T++++ D DG+G +  DEFV  F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  VEGVG----EEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKF 127
             G+G        V+DLKEAF +Y++DG G I+ K L  ++  LGE  S+ DCKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLDSDGVLNFDEFKFMMS 141
           D+D DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

BLAST of CsGy2G025350 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-26
Identity = 66/136 (48.53%), Postives = 90/136 (66.18%), Query Frame = 0

Query: 10  VFKQVDGDGDGKLSPPELQRCI-LGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVE 69
           +F   D DGDG++S  EL+ C+   +G  ++ EEA  +V  +D+DGDGL+   EFV  V+
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  GVGEEE----KVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKFDL 129
               EE    +   L+EAF MYEM+G G IT  SL+RML +LG  + IDDC+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 DSDGVLNFDEFKFMMS 141
           + DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

BLAST of CsGy2G025350 vs. NCBI nr
Match: XP_004138612.1 (calcium-binding protein CML38 [Cucumis sativus] >KGN63180.1 hypothetical protein Csa_021832 [Cucumis sativus])

HSP 1 Score: 281 bits (719), Expect = 1.89e-95
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
           DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CsGy2G025350 vs. NCBI nr
Match: XP_008441354.1 (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0056590.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa] >TYK24272.1 putative calcium-binding protein CML19 [Cucumis melo var. makuwa])

HSP 1 Score: 278 bits (710), Expect = 4.45e-94
Identity = 138/140 (98.57%), Postives = 140/140 (100.00%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
           DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI+DCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CsGy2G025350 vs. NCBI nr
Match: XP_038886217.1 (putative calcium-binding protein CML19 [Benincasa hispida])

HSP 1 Score: 271 bits (692), Expect = 2.48e-91
Identity = 134/140 (95.71%), Postives = 137/140 (97.86%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRC+ GVGG LTIEEAETVVE LDSDGDGL+GW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCMAGVGGVLTIEEAETVVENLDSDGDGLLGW 60

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
           DEFVEFVEGVGEEEKV+DLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CsGy2G025350 vs. NCBI nr
Match: XP_022938684.1 (putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 putative calcium-binding protein CML19 isoform X2 [Cucurbita pepo subsp. pepo] >KAG7015957.1 putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 248 bits (634), Expect = 1.79e-82
Identity = 122/140 (87.14%), Postives = 132/140 (94.29%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+ GVGGSLT+EEAE VVE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
            EFV FVEGVGEEEKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSI++C KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDL+SDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

BLAST of CsGy2G025350 vs. NCBI nr
Match: XP_022992628.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 248 bits (633), Expect = 2.55e-82
Identity = 122/140 (87.14%), Postives = 132/140 (94.29%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+ GVGGSLT+EEAE  VE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
            EFV FVE VGEEEKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSI++CKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

BLAST of CsGy2G025350 vs. ExPASy TrEMBL
Match: A0A0A0LMM5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G406780 PE=4 SV=1)

HSP 1 Score: 281 bits (719), Expect = 9.13e-96
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
           DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CsGy2G025350 vs. ExPASy TrEMBL
Match: A0A5A7UNE9 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00360 PE=4 SV=1)

HSP 1 Score: 278 bits (710), Expect = 2.15e-94
Identity = 138/140 (98.57%), Postives = 140/140 (100.00%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
           DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI+DCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CsGy2G025350 vs. ExPASy TrEMBL
Match: A0A1S3B3Z8 (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103485494 PE=4 SV=1)

HSP 1 Score: 278 bits (710), Expect = 2.15e-94
Identity = 138/140 (98.57%), Postives = 140/140 (100.00%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
           DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI+DCKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDLDSDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CsGy2G025350 vs. ExPASy TrEMBL
Match: A0A6J1FET5 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111444836 PE=4 SV=1)

HSP 1 Score: 248 bits (634), Expect = 8.67e-83
Identity = 122/140 (87.14%), Postives = 132/140 (94.29%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+ GVGGSLT+EEAE VVE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMVVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
            EFV FVEGVGEEEKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSI++C KMIA
Sbjct: 62  GEFVAFVEGVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECNKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDL+SDGVLNFDEF FMMS
Sbjct: 122 KFDLNSDGVLNFDEFMFMMS 141

BLAST of CsGy2G025350 vs. ExPASy TrEMBL
Match: A0A6J1JZS3 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111488916 PE=4 SV=1)

HSP 1 Score: 248 bits (633), Expect = 1.23e-82
Identity = 122/140 (87.14%), Postives = 132/140 (94.29%), Query Frame = 0

Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60
           MDK+ALYEKVFKQVDGDGDGKLSP ELQRC+ GVGGSLT+EEAE  VE LD+DGDGL+GW
Sbjct: 2   MDKMALYEKVFKQVDGDGDGKLSPLELQRCMAGVGGSLTLEEAEMAVENLDTDGDGLMGW 61

Query: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120
            EFV FVE VGEEEKV+DLKEAFRMYEMDGCGFITTKSLKRMLS+LGESRSI++CKKMIA
Sbjct: 62  GEFVAFVEEVGEEEKVSDLKEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 121

Query: 121 KFDLDSDGVLNFDEFKFMMS 140
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 122 KFDLNSDGVLNFDEFKFMMS 141

BLAST of CsGy2G025350 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 132.1 bits (331), Expect = 3.5e-31
Identity = 68/138 (49.28%), Postives = 94/138 (68.12%), Query Frame = 0

Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+  + +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAK 121
           EF + ++   EEEK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ DDC+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLDSDGVLNFDEFKFMM 140
           FDL++DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

BLAST of CsGy2G025350 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 132.1 bits (331), Expect = 3.5e-31
Identity = 68/138 (49.28%), Postives = 94/138 (68.12%), Query Frame = 0

Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+  + +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAK 121
           EF + ++   EEEK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ DDC+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLDSDGVLNFDEFKFMM 140
           FDL++DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

BLAST of CsGy2G025350 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 128.6 bits (322), Expect = 3.8e-30
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 10  VFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFVEG 69
           VF  +D + DGK+S  ELQ C+  +GG+L+  E E VV+  D DGDG + ++EF++ +EG
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  V--GEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKFDLDSD 129
               +EE+  +LKEAF MY M+G  FIT  SL+R LS+LGES ++D CK MI  FD + D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

BLAST of CsGy2G025350 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 122.1 bits (305), Expect = 3.6e-28
Identity = 64/138 (46.38%), Postives = 91/138 (65.94%), Query Frame = 0

Query: 8   EKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVE-F 67
           +KVF++ D +GDGK+S  EL+  I  +  + + EE  T++++ D DG+G +  DEFV  F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  VEGVG----EEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKF 127
             G+G        V+DLKEAF +Y++DG G I+ K L  ++  LGE  S+ DCKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 128 DLDSDGVLNFDEFKFMMS 141
           D+D DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

BLAST of CsGy2G025350 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.0e-27
Identity = 67/135 (49.63%), Postives = 87/135 (64.44%), Query Frame = 0

Query: 8   EKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGWDEFVEFV 67
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++  +EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 68  EG---VGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIAKFDL 127
           +G     EEEK   + EAFRMY  DG   IT  SLK ML KLGESR+ DDCK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 128 DSDGVLNFDEFKFMM 140
           ++DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRE64.9e-3049.28Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1[more]
Q8RYJ82.4e-2949.64Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9FIH95.4e-2950.76Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1[more]
P250705.1e-2746.38Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2[more]
Q8RYK01.1e-2648.53Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
XP_004138612.11.89e-95100.00calcium-binding protein CML38 [Cucumis sativus] >KGN63180.1 hypothetical protein... [more]
XP_008441354.14.45e-9498.57PREDICTED: putative calcium-binding protein CML19 [Cucumis melo] >KAA0056590.1 p... [more]
XP_038886217.12.48e-9195.71putative calcium-binding protein CML19 [Benincasa hispida][more]
XP_022938684.11.79e-8287.14putative calcium-binding protein CML19 [Cucurbita moschata] >XP_023550776.1 puta... [more]
XP_022992628.12.55e-8287.14putative calcium-binding protein CML19 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0LMM59.13e-96100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G406780 PE=4 SV=1[more]
A0A5A7UNE92.15e-9498.57Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3Z82.15e-9498.57putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103485494 P... [more]
A0A6J1FET58.67e-8387.14putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1JZS31.23e-8287.14putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114889... [more]
Match NameE-valueIdentityDescription
AT1G76650.13.5e-3149.28calmodulin-like 38 [more]
AT1G76650.33.5e-3149.28calmodulin-like 38 [more]
AT5G42380.13.8e-3050.76calmodulin like 37 [more]
AT5G37770.13.6e-2846.38EF hand calcium-binding protein family [more]
AT1G76640.11.0e-2749.63Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 114..140
e-value: 1.1
score: 17.5
coord: 42..70
e-value: 11.0
score: 9.4
coord: 6..34
e-value: 0.75
score: 18.8
coord: 78..106
e-value: 0.19
score: 20.8
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 78..139
e-value: 1.8E-10
score: 41.1
coord: 6..67
e-value: 9.6E-14
score: 51.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 38..73
score: 13.034948
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 110..140
score: 14.373928
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 74..109
score: 11.361225
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 2..37
score: 12.672309
IPR002048EF-hand domainCDDcd00051EFhcoord: 78..140
e-value: 2.56588E-14
score: 61.4097
NoneNo IPR availableGENE3D1.10.238.10coord: 1..71
e-value: 3.8E-15
score: 57.8
NoneNo IPR availableGENE3D1.10.238.10coord: 72..140
e-value: 7.0E-19
score: 69.8
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 2..140
NoneNo IPR availablePANTHERPTHR10891:SF858EF HAND CALCIUM-BINDING FAMILY PROTEINcoord: 2..140
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 123..135
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 15..27
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 51..63
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 3..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G025350.1CsGy2G025350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding