CsGy2G025045 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G025045
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionFibroin heavy chain
LocationGy14Chr2: 32635176 .. 32635975 (-)
RNA-Seq ExpressionCsGy2G025045
SyntenyCsGy2G025045
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATGGGATAAGAAATATGTGAAGAACAACAAACATAGAGTTCAAAATTCACATCTCTCTCTCACTCTCTCTTTTTCATCCTCTCACTATGAAATCAGACTCCTCCAATTCCCCATGGAACAACTTCCCCACCATTTTCAGTTTCGACCAGGACCGCCGCAACACAAAGGAACTACCAGCCTTAGTCTTGGAAGCCGGGGGAGGAGCAGGAGTTGGGTGCGGCCTTGGAATCGGGTTCGGACTCGTGGGAGGGATCGGCCACGCCGGTGCCTCGCCCTGGAATCACCTTCACCTCGTATTTGGCCTGGGTGCCGGCTGTGGCGTTGGGTTAGGGCTTGGAATTGGGCAAGGCTTTGGATATGGCGTCTCCTTTCAATCTGTTGATTCTTATTTCTCTCATCTCATTTCTAACCCTAAACCTAAGCAGCCTTCCCTCATTCAATTTTGAAATTTCCTCTCTTACCCATTTCCTTACCCTTTCATTTTTATCTTCTCCTTTCAGAATTTTTTTTTTGCATCTTTCAACATCTTTTTCGACTTCTTATTTCTCCATTTAATTTGTATGCATTAATTGCACATTCATTTTATTAATCCACCACTATCAAATTCTTCAACTAATATTAATCATTTTCATATTTGTGAGTATGCTTTTATAGTAAAAATGTTAGTAAACTAGCATGGTGACATGAACAACAATTCAGTTTACAAAAGAAAAATGGTTACCGTAACCCTAATTTGAGTGGTAGCATATATCCATAGGCTTATATAAAACTTAAATTAAAAAACTGACCAACAATTCA

mRNA sequence

CGATGGGATAAGAAATATGTGAAGAACAACAAACATAGAGTTCAAAATTCACATCTCTCTCTCACTCTCTCTTTTTCATCCTCTCACTATGAAATCAGACTCCTCCAATTCCCCATGGAACAACTTCCCCACCATTTTCAGTTTCGACCAGGACCGCCGCAACACAAAGGAACTACCAGCCTTAGTCTTGGAAGCCGGGGGAGGAGCAGGAGTTGGGTGCGGCCTTGGAATCGGGTTCGGACTCGTGGGAGGGATCGGCCACGCCGGTGCCTCGCCCTGGAATCACCTTCACCTCGTATTTGGCCTGGGTGCCGGCTGTGGCGTTGGGTTAGGGCTTGGAATTGGGCAAGGCTTTGGATATGGCGTCTCCTTTCAATCTGTTGATTCTTATTTCTCTCATCTCATTTCTAACCCTAAACCTAAGCAGCCTTCCCTCATTCAATTTTGAAATTTCCTCTCTTACCCATTTCCTTACCCTTTCATTTTTATCTTCTCCTTTCAGAATTTTTTTTTTGCATCTTTCAACATCTTTTTCGACTTCTTATTTCTCCATTTAATTTGTATGCATTAATTGCACATTCATTTTATTAATCCACCACTATCAAATTCTTCAACTAATATTAATCATTTTCATATTTGTGAGTATGCTTTTATAGTAAAAATGTTAGTAAACTAGCATGGTGACATGAACAACAATTCAGTTTACAAAAGAAAAATGGTTACCGTAACCCTAATTTGAGTGGTAGCATATATCCATAGGCTTATATAAAACTTAAATTAAAAAACTGACCAACAATTCA

Coding sequence (CDS)

ATGAAATCAGACTCCTCCAATTCCCCATGGAACAACTTCCCCACCATTTTCAGTTTCGACCAGGACCGCCGCAACACAAAGGAACTACCAGCCTTAGTCTTGGAAGCCGGGGGAGGAGCAGGAGTTGGGTGCGGCCTTGGAATCGGGTTCGGACTCGTGGGAGGGATCGGCCACGCCGGTGCCTCGCCCTGGAATCACCTTCACCTCGTATTTGGCCTGGGTGCCGGCTGTGGCGTTGGGTTAGGGCTTGGAATTGGGCAAGGCTTTGGATATGGCGTCTCCTTTCAATCTGTTGATTCTTATTTCTCTCATCTCATTTCTAACCCTAAACCTAAGCAGCCTTCCCTCATTCAATTTTGA

Protein sequence

MKSDSSNSPWNNFPTIFSFDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF*
Homology
BLAST of CsGy2G025045 vs. NCBI nr
Match: KGN63146.1 (hypothetical protein Csa_022245 [Cucumis sativus])

HSP 1 Score: 236 bits (603), Expect = 1.95e-78
Identity = 118/119 (99.16%), Postives = 118/119 (99.16%), Query Frame = 0

Query: 1   MKSDSSNSPWNNFPTIFSFDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAG 60
           MKSDSSNSPWNNFPTIF FDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAG
Sbjct: 1   MKSDSSNSPWNNFPTIFCFDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAG 60

Query: 61  ASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF 119
           ASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF
Sbjct: 61  ASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF 119

BLAST of CsGy2G025045 vs. NCBI nr
Match: KAA0051071.1 (fibroin heavy chain [Cucumis melo var. makuwa] >TYK02741.1 fibroin heavy chain [Cucumis melo var. makuwa])

HSP 1 Score: 206 bits (525), Expect = 1.69e-66
Identity = 108/122 (88.52%), Postives = 112/122 (91.80%), Query Frame = 0

Query: 1   MKSDSSNSPWNNF-PTIFSFDQDRRNTKELPAL--VLEAGGGAGVGCGLGIGFGLVGGIG 60
           MKSDSS+SPWNNF  TIFSFD DRRNTKELPAL  VLEAGGGAGVGCGLG GFGLVGGIG
Sbjct: 1   MKSDSSSSPWNNFHTTIFSFDHDRRNTKELPALALVLEAGGGAGVGCGLGFGFGLVGGIG 60

Query: 61  HAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLI 119
           H GASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYG SF+S+DSYFSHL S+PK KQPSLI
Sbjct: 61  HGGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGFSFESLDSYFSHLNSDPKHKQPSLI 120

BLAST of CsGy2G025045 vs. NCBI nr
Match: KAG6578355.1 (hypothetical protein SDJN03_22803, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 156 bits (394), Expect = 2.19e-46
Identity = 91/134 (67.91%), Postives = 100/134 (74.63%), Query Frame = 0

Query: 1   MKSDSSNSPW-------NNFPTI-----FS----FDQDRRNTKELPALVLEAGGGAGVGC 60
           MKSDS NSPW       NNF T      FS    FD +RRN K+ P LVL+AGGGAGVGC
Sbjct: 1   MKSDS-NSPWKWNTAITNNFHTKADHHHFSLPNIFDANRRNAKQPPPLVLDAGGGAGVGC 60

Query: 61  GLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSH 118
           G+G+GFGLVGGIGH GASPWNHLHLVFGLG GCGVGLGLGIG+G GYG+SF S+DSYFS 
Sbjct: 61  GVGLGFGLVGGIGHGGASPWNHLHLVFGLGLGCGVGLGLGIGKGIGYGISFDSLDSYFSD 120

BLAST of CsGy2G025045 vs. NCBI nr
Match: XP_007033168.1 (PREDICTED: keratin-associated protein 21-1 [Theobroma cacao] >EOY04094.1 Uncharacterized protein TCM_019361 [Theobroma cacao])

HSP 1 Score: 100 bits (250), Expect = 1.03e-24
Identity = 50/79 (63.29%), Postives = 62/79 (78.48%), Query Frame = 0

Query: 23  RRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLG 82
           R + +++   VL  GGGAG+GCG+G+GFGLVGGIG+ G  PWNHL L FG+GAGCGVGLG
Sbjct: 34  RESRQKVEENVLGPGGGAGIGCGIGVGFGLVGGIGYGG-WPWNHLKLAFGVGAGCGVGLG 93

Query: 83  LGIGQGFGYGVSFQSVDSY 101
            G GQG GYG S +S++SY
Sbjct: 94  FGFGQGIGYGFSLESLESY 111

BLAST of CsGy2G025045 vs. NCBI nr
Match: XP_022769621.1 (keratin-associated protein 21-1 [Durio zibethinus])

HSP 1 Score: 100 bits (249), Expect = 1.88e-24
Identity = 52/90 (57.78%), Postives = 66/90 (73.33%), Query Frame = 0

Query: 23  RRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLG 82
           R + +++   VL  G GAG+GCG+G+GFGLVGG+G+ G  PWNHL LVFG+GAGCGVG+G
Sbjct: 34  RESRQKVGENVLGPGCGAGIGCGIGVGFGLVGGVGYGG-WPWNHLKLVFGVGAGCGVGIG 93

Query: 83  LGIGQGFGYGVSFQSVDSYFSHLISNPKPK 112
            G GQG GYG S +S++SY S   SN   K
Sbjct: 94  FGFGQGIGYGFSLESLESYLSGDSSNSNRK 122

BLAST of CsGy2G025045 vs. ExPASy TrEMBL
Match: A0A0A0LSZ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G404970 PE=4 SV=1)

HSP 1 Score: 236 bits (603), Expect = 9.43e-79
Identity = 118/119 (99.16%), Postives = 118/119 (99.16%), Query Frame = 0

Query: 1   MKSDSSNSPWNNFPTIFSFDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAG 60
           MKSDSSNSPWNNFPTIF FDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAG
Sbjct: 1   MKSDSSNSPWNNFPTIFCFDQDRRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAG 60

Query: 61  ASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF 119
           ASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF
Sbjct: 61  ASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLIQF 119

BLAST of CsGy2G025045 vs. ExPASy TrEMBL
Match: A0A5A7UC28 (Fibroin heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154G00440 PE=4 SV=1)

HSP 1 Score: 206 bits (525), Expect = 8.20e-67
Identity = 108/122 (88.52%), Postives = 112/122 (91.80%), Query Frame = 0

Query: 1   MKSDSSNSPWNNF-PTIFSFDQDRRNTKELPAL--VLEAGGGAGVGCGLGIGFGLVGGIG 60
           MKSDSS+SPWNNF  TIFSFD DRRNTKELPAL  VLEAGGGAGVGCGLG GFGLVGGIG
Sbjct: 1   MKSDSSSSPWNNFHTTIFSFDHDRRNTKELPALALVLEAGGGAGVGCGLGFGFGLVGGIG 60

Query: 61  HAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYFSHLISNPKPKQPSLI 119
           H GASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYG SF+S+DSYFSHL S+PK KQPSLI
Sbjct: 61  HGGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGFSFESLDSYFSHLNSDPKHKQPSLI 120

BLAST of CsGy2G025045 vs. ExPASy TrEMBL
Match: A0A061EP29 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_019361 PE=4 SV=1)

HSP 1 Score: 100 bits (250), Expect = 5.00e-25
Identity = 50/79 (63.29%), Postives = 62/79 (78.48%), Query Frame = 0

Query: 23  RRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLG 82
           R + +++   VL  GGGAG+GCG+G+GFGLVGGIG+ G  PWNHL L FG+GAGCGVGLG
Sbjct: 34  RESRQKVEENVLGPGGGAGIGCGIGVGFGLVGGIGYGG-WPWNHLKLAFGVGAGCGVGLG 93

Query: 83  LGIGQGFGYGVSFQSVDSY 101
            G GQG GYG S +S++SY
Sbjct: 94  FGFGQGIGYGFSLESLESY 111

BLAST of CsGy2G025045 vs. ExPASy TrEMBL
Match: A0A6P6AXV7 (keratin-associated protein 21-1 OS=Durio zibethinus OX=66656 GN=LOC111313209 PE=4 SV=1)

HSP 1 Score: 100 bits (249), Expect = 9.12e-25
Identity = 52/90 (57.78%), Postives = 66/90 (73.33%), Query Frame = 0

Query: 23  RRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLG 82
           R + +++   VL  G GAG+GCG+G+GFGLVGG+G+ G  PWNHL LVFG+GAGCGVG+G
Sbjct: 34  RESRQKVGENVLGPGCGAGIGCGIGVGFGLVGGVGYGG-WPWNHLKLVFGVGAGCGVGIG 93

Query: 83  LGIGQGFGYGVSFQSVDSYFSHLISNPKPK 112
            G GQG GYG S +S++SY S   SN   K
Sbjct: 94  FGFGQGIGYGFSLESLESYLSGDSSNSNRK 122

BLAST of CsGy2G025045 vs. ExPASy TrEMBL
Match: A0A7N2KWH8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 100 bits (249), Expect = 1.27e-24
Identity = 50/89 (56.18%), Postives = 64/89 (71.91%), Query Frame = 0

Query: 25  NTKELPALVLEAGGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLGLG 84
           N K+   +VL  GGG G+GCG+G+GFGLVGG+G+ G  PWNHL LV G+G GCGVG+G G
Sbjct: 46  NPKKAEEIVLGPGGGVGIGCGVGLGFGLVGGVGYGG-WPWNHLQLVIGIGIGCGVGVGFG 105

Query: 85  IGQGFGYGVSFQSVDSYFSHLISNPKPKQ 113
            GQG GYG S +S++SY S   S+   K+
Sbjct: 106 YGQGIGYGFSLESLESYLSKQSSSDSKKR 133

BLAST of CsGy2G025045 vs. TAIR 10
Match: AT1G66820.1 (glycine-rich protein )

HSP 1 Score: 62.0 bits (149), Expect = 3.8e-10
Identity = 36/105 (34.29%), Postives = 51/105 (48.57%), Query Frame = 0

Query: 5   SSNSPWNNFPTIFSFDQD-------RRNTKELPALVLEAGGGAGVGCGLGIGFGLVGGIG 64
           S+N  W+  P + +   +       R     +    +  G G G+GCG GIG GL GG+G
Sbjct: 3   STNKLWSRKPDVKTGKSEFPVTRFLRTQIDNIKTTTVGPGIGGGIGCGAGIGIGLSGGLG 62

Query: 65  HAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSVDSYF 103
              +   +H ++V G G GCG+G G G G G G G SF  +   F
Sbjct: 63  IGASEGLDHSNVVLGFGIGCGIGFGFGYGFGVGGGYSFDDIKERF 107

BLAST of CsGy2G025045 vs. TAIR 10
Match: AT4G10330.1 (glycine-rich protein )

HSP 1 Score: 53.1 bits (126), Expect = 1.7e-07
Identity = 30/65 (46.15%), Postives = 38/65 (58.46%), Query Frame = 0

Query: 39  GAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQSV 98
           G GVGCG G G GL+GG+G     P     L FGLG G G G+G+G G G G G ++   
Sbjct: 34  GLGVGCGFGFGAGLIGGVGFGPGVP----GLQFGLGFGAGCGIGVGFGYGVGRGAAYDHS 93

Query: 99  DSYFS 104
            SY++
Sbjct: 94  RSYYN 94

BLAST of CsGy2G025045 vs. TAIR 10
Match: AT1G27695.1 (glycine-rich protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.1e-04
Identity = 29/61 (47.54%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 37 GGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQ 96
          G G GVGCG G+G+G        G  P N L +  G G GCGVGLGLG G G  +G  ++
Sbjct: 12 GFGFGVGCGFGVGWGF-------GGMPMNILGV--GAGGGCGVGLGLGWGFGTAFGSHYR 63

Query: 97 S 98
          S
Sbjct: 72 S 63

BLAST of CsGy2G025045 vs. TAIR 10
Match: AT1G27695.2 (glycine-rich protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.1e-04
Identity = 29/61 (47.54%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 37 GGGAGVGCGLGIGFGLVGGIGHAGASPWNHLHLVFGLGAGCGVGLGLGIGQGFGYGVSFQ 96
          G G GVGCG G+G+G        G  P N L +  G G GCGVGLGLG G G  +G  ++
Sbjct: 12 GFGFGVGCGFGVGWGF-------GGMPMNILGV--GAGGGCGVGLGLGWGFGTAFGSHYR 63

Query: 97 S 98
          S
Sbjct: 72 S 63

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KGN63146.11.95e-7899.16hypothetical protein Csa_022245 [Cucumis sativus][more]
KAA0051071.11.69e-6688.52fibroin heavy chain [Cucumis melo var. makuwa] >TYK02741.1 fibroin heavy chain [... [more]
KAG6578355.12.19e-4667.91hypothetical protein SDJN03_22803, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_007033168.11.03e-2463.29PREDICTED: keratin-associated protein 21-1 [Theobroma cacao] >EOY04094.1 Unchara... [more]
XP_022769621.11.88e-2457.78keratin-associated protein 21-1 [Durio zibethinus][more]
Match NameE-valueIdentityDescription
A0A0A0LSZ29.43e-7999.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G404970 PE=4 SV=1[more]
A0A5A7UC288.20e-6788.52Fibroin heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1154... [more]
A0A061EP295.00e-2563.29Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_019361 PE=4 SV=1[more]
A0A6P6AXV79.12e-2557.78keratin-associated protein 21-1 OS=Durio zibethinus OX=66656 GN=LOC111313209 PE=... [more]
A0A7N2KWH81.27e-2456.18Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G66820.13.8e-1034.29glycine-rich protein [more]
AT4G10330.11.7e-0746.15glycine-rich protein [more]
AT1G27695.11.1e-0447.54glycine-rich protein [more]
AT1G27695.21.1e-0447.54glycine-rich protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34201GLYCINE-RICH PROTEINcoord: 7..111
NoneNo IPR availablePANTHERPTHR34201:SF6GLYCINE-RICH PROTEINcoord: 7..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G025045.1CsGy2G025045.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane