CsGy2G021120 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy2G021120
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	dihydrofolate reductase-like
Location	Gy14Chr2: 29821219 .. 29824135 (+)
RNA-Seq Expression	CsGy2G021120
Synteny	CsGy2G021120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATAAATATATTAAAATTAAACAAACACACTGCCGTCAGCACGTTCGTTTTCTTTCTTTCTTTTTTAAAACGAGTCCCAATCAGAAGTTTCCCGGTGAGGAGGAAGACGGAAGGATGAATGCTCTACCAATTGCTTTTTAAATGCCTCCATTCTTCCCTTCCCTCTCTTCACATCAATCTCTCACTACAATTCCACAATTTCGTTTCCCCACGATTCCAATCATGGGAAGCGATCAAAAATTCTTCACAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGTACCAGTGCAGCAATCCTCAAGAAGCAGGTCGGAAAATGGCCGCTTTCTGTTCTCGATCAACTCGATCTCCATTTCCTCGACGCTCCCTTTCCCGCTGAAGGAAAGTCCGACGTCGAAGGAATCTTTGATCCTCCTTATTTTGAGTGGTTCCAATTCAGTCCAGTACGATTTTGCTTCCCCTAATTTCGATTTCCTTGTAAACCTCTCCATTAATTTTTTTAATTTTTATTTTTGTCCCTTTATTTTTCCATTACAGGAGTTCACGGAATATAGGAATTTCGACGAGTGTCTCTCATTCATTGAGAATTACATGATTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGTAATTTCGTCTTCCTCTAATTCTTTTCCTAATTACTTAAGCGGATCTTAATCGAGTGTCAAATTGTTGTTGAATTTCGCTATTTCAGGGGGCGATACTATCCGCGGCTCTCCCTGGATTTCAAGCTAAGGTACCCTAATTAACAACAGCTTCCTCTGTTCTGAGCTATTAATACAATTATTTCATTTGAATTCTCTAAAAATAAGATTCTAAGGCGTCGGTGCGGTTGATTAATGGTTGCAGGGAATTGCTCTGACGAAAGTCCCGAAGATCAAATTCGTGATCATCGTGAGCGGTGCGAAATTCAGATCGGAATCTGTGGCTGAGAAAGCTTACTCGACTCCAATTGGATGCCCATCCCTCCATTTTCTAGGTACCTCCGCATGATTTCTTTTGTCCTTACCCTCTGCTTTTTCAAATATTTTGATTCCTTCCTTTGTTTTTTCCATAACAAACAAACCACTCAACAAAATTTGTCATTAATCTATAATTTTCTACCAGATATACAGATCGTTGCAATTGACTATTTTTCTACATTTCTCAGTGTCTTCTCGTTAAACTATACGTTACTCTACTCATTCACTTCTCGACCATCTAAGTAGTTTTCATAGGAAAAGATAATGATTTTATTAAAATGAATTTGAGGGAATTAAGTCCATGAAGAATATAAATTATTATTATATTCTTCTAAAAGTAAATAAAAAAAGAAAAAGGCTAATTGATTTCTAATGGTCCTGGCTGAACATGGGAAGTTGTTAAATGTGAAATCACATTTATTATTTAATTTAAGATGCACTTATTTGTTTTGAAATGTTTGATTACAACTTTCATTCTAAAATTATAATTATTTAATCAACTTATAAAACTTATGCTTAAAGAAATTTTGTAAAATAATGGTGGCAGGTGAGGAAGACTTCTTAATGCCACATGGGAAGAAGCTTCTGGAATCATATATAGAACCAACAATTATTACTCATCCAAAAGGCCATACAATACCAAGACTAGGTAATACAATATTTAAACTAATTTCACTTTTGCTTAAATACATATTTACTTGCTTAATTTCTTATGCTAATTTATATTAATTTCTTCATTTTATTTATATTTTTAGATGATAAAGCTTTGGAGGTTATGGAGAGTTTCATTCACAGGATTTCAAAGATCTTAAATGAGAATGAAGAATAGATGTCTTATTTTTACTCATGGAAAAAAAAAAAGAATTATGTTTATTGTATGATAATTTTGTAATATAATCTTCATGTTTATCGCAATATATGTAATAATGGAAAAAAAATGATGATGTTGAGACGAGTTTAATCTCCCAATGTCAGGTTAAAGTTATTGGTAGTTTAACTTGATTCAACTTCTTCAAAAATTATTTATTCATTTCATGGAAAAGAAAACTATTAACGTTGCTTTTATAATTTTGAAAAGATATTTACACTAAATTTTCTCTCTC

mRNA sequence

AATAAATATATTAAAATTAAACAAACACACTGCCGTCAGCACGTTCGTTTTCTTTCTTTCTTTTTTAAAACGAGTCCCAATCAGAAGTTTCCCGGTGAGGAGGAAGACGGAAGGATGAATGCTCTACCAATTGCTTTTTAAATGCCTCCATTCTTCCCTTCCCTCTCTTCACATCAATCTCTCACTACAATTCCACAATTTCGTTTCCCCACGATTCCAATCATGGGAAGCGATCAAAAATTCTTCACAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGTACCAGTGCAGCAATCCTCAAGAAGCAGGTCGGAAAATGGCCGCTTTCTGTTCTCGATCAACTCGATCTCCATTTCCTCGACGCTCCCTTTCCCGCTGAAGGAAAGTCCGACGTCGAAGGAATCTTTGATCCTCCTTATTTTGAGTGGTTCCAATTCAGTCCAGAGTTCACGGAATATAGGAATTTCGACGAGTGTCTCTCATTCATTGAGAATTACATGATTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGGGGCGATACTATCCGCGGCTCTCCCTGGATTTCAAGCTAAGGGAATTGCTCTGACGAAAGTCCCGAAGATCAAATTCGTGATCATCGTGAGCGGTGCGAAATTCAGATCGGAATCTGTGGCTGAGAAAGCTTACTCGACTCCAATTGGATGCCCATCCCTCCATTTTCTAGGTGAGGAAGACTTCTTAATGCCACATGGGAAGAAGCTTCTGGAATCATATATAGAACCAACAATTATTACTCATCCAAAAGGCCATACAATACCAAGACTAGATGATAAAGCTTTGGAGGTTATGGAGAGTTTCATTCACAGGATTTCAAAGATCTTAAATGAGAATGAAGAATAGATGTCTTATTTTTACTCATGGAAAAAAAAAAAGAATTATGTTTATTGTATGATAATTTTGTAATATAATCTTCATGTTTATCGCAATATATGTAATAATGGAAAAAAAATGATGATGTTGAGACGAGTTTAATCTCCCAATGTCAGGTTAAAGTTATTGGTAGTTTAACTTGATTCAACTTCTTCAAAAATTATTTATTCATTTCATGGAAAAGAAAACTATTAACGTTGCTTTTATAATTTTGAAAAGATATTTACACTAAATTTTCTCTCTC

Coding sequence (CDS)

ATGCCTCCATTCTTCCCTTCCCTCTCTTCACATCAATCTCTCACTACAATTCCACAATTTCGTTTCCCCACGATTCCAATCATGGGAAGCGATCAAAAATTCTTCACAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGTACCAGTGCAGCAATCCTCAAGAAGCAGGTCGGAAAATGGCCGCTTTCTGTTCTCGATCAACTCGATCTCCATTTCCTCGACGCTCCCTTTCCCGCTGAAGGAAAGTCCGACGTCGAAGGAATCTTTGATCCTCCTTATTTTGAGTGGTTCCAATTCAGTCCAGAGTTCACGGAATATAGGAATTTCGACGAGTGTCTCTCATTCATTGAGAATTACATGATTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGGGGCGATACTATCCGCGGCTCTCCCTGGATTTCAAGCTAAGGGAATTGCTCTGACGAAAGTCCCGAAGATCAAATTCGTGATCATCGTGAGCGGTGCGAAATTCAGATCGGAATCTGTGGCTGAGAAAGCTTACTCGACTCCAATTGGATGCCCATCCCTCCATTTTCTAGGTGAGGAAGACTTCTTAATGCCACATGGGAAGAAGCTTCTGGAATCATATATAGAACCAACAATTATTACTCATCCAAAAGGCCATACAATACCAAGACTAGATGATAAAGCTTTGGAGGTTATGGAGAGTTTCATTCACAGGATTTCAAAGATCTTAAATGAGAATGAAGAATAG

Protein sequence

MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE*

Homology

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BLAST of CsGy2G021120 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 4.0e-19
Identity = 66/208 (31.73%), Postives = 98/208 (47.12%), Query Frame = 0


Query: 36  TKP-RFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSD---------- 95
           +KP + LCLHG+  S  +  K++G     +    +LHF   P  A+ ++D          
Sbjct: 2   SKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRL 61

Query: 96  --VEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAAL 155
             + G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A L
Sbjct: 62  AALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAML 121

Query: 156 PGFQAKG---IALTKVPKIKFVIIVSGAKFRSESVA-EKAYSTPIGCPSLHFLGEEDFLM 215
                 G       + P  KFV+ V G  FR+E    +  Y+  +  PSLH  G  D L+
Sbjct: 122 AQMLQPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAGTSDTLV 181

Query: 216 P--HGKKLLESYIEPTIITHPKGHTIPR 225
           P    K+L+E      ++ HP  H +P+
Sbjct: 182 PLARSKQLVERCENAHVLLHPGQHIVPQ 207

[Close]

BLAST of CsGy2G021120 vs. ExPASy Swiss-Prot
Match: Q7QBJ0 (Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3)

HSP 1 Score: 89.4 bits (220), Expect = 6.5e-17
Identity = 62/199 (31.16%), Postives = 94/199 (47.24%), Query Frame = 0


Query: 37  KPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEW 96
           K + L LHG+R +A   K ++G +   +   ++  F+ AP PA     V G  DP    W
Sbjct: 11  KLKVLALHGYRQNADSFKSKLGSFRKMLNKYVEFVFVSAPHPAAPLEAVGGEPDPNQRSW 70

Query: 97  FQFSPEFTEYR---------NFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 156
           + F+ +   ++          FDE L  +E      G   G LGFSQGA     L    A
Sbjct: 71  W-FNKDDRTFKGTNQGGPAYGFDESLRLVERTWQAEG-CHGLLGFSQGACFVGLLCDLSA 130

Query: 157 KGIALTKVPKIKFVIIVSGAKFRSESVAE-KAYSTPIGCPSLHFLGEEDFLMPH--GKKL 216
           +G+   K    +F ++ SG  FRS S+     Y   +  PSLH  GE D ++     + L
Sbjct: 131 RGMTTMKP---QFAVVASG--FRSGSLVHLNYYENKVQIPSLHIFGETDEIITKDMSEAL 190

Query: 217 LESYIEPTIITHPKGHTIP 224
            E++++P ++THP GH  P
Sbjct: 191 AETFLDPEVVTHPGGHYFP 202

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BLAST of CsGy2G021120 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 63/204 (30.88%), Postives = 88/204 (43.14%), Query Frame = 0


Query: 39  RFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGI---------F 98
           R LCL GFR S    +++ G    ++  + +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 99  DPPYFEWFQFS--------PEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAA 158
           + P   WF            E T  R  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 159 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMP-- 218
           +      G    + P  +FVI+VSG   R   + E     P+  PSLH  G+ D ++P  
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGDTDGVIPSQ 187

Query: 219 HGKKLLESYIEPTIITHPKGHTIP 224
              +L   +     +TH  GH IP
Sbjct: 188 ESMQLCSRFDGAVTLTHSGGHFIP 209

[Close]

BLAST of CsGy2G021120 vs. ExPASy Swiss-Prot
Match: Q0C7C4 (Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-15
Identity = 67/226 (29.65%), Postives = 103/226 (45.58%), Query Frame = 0


Query: 36  TKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFE 95
           +K + L LHG+R +    K ++G +   +    +L F+ AP  A    D E   +P   +
Sbjct: 23  SKLKILALHGYRQNGDGFKSKLGSFRKFIGKHAELVFVTAPHIAPPLPDSEAGTEPDPAQ 82

Query: 96  ---WFQFSPEFTEYRN-------FDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGF 155
              WF       +  N       F++ L  +E  + K     G LGFSQGA     L   
Sbjct: 83  RSWWFNKDDGTFKGTNKNGPAIGFEDSLKLVEK-VWKQEQCCGLLGFSQGACFVGLLCDL 142

Query: 156 QAKGIALTKVPKIKFVIIVSGAKFRSESVAE-KAYSTPIGCPSLHFLGEEDFLMPH--GK 215
            A+G  +T + K +F ++ SG  FRS S+     Y T +  PSLH  GE D ++P     
Sbjct: 143 SARG--MTSI-KPEFAVLSSG--FRSGSLVHLNCYETKVQIPSLHIYGEADEIIPKEMSM 202

Query: 216 KLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENE 249
            L +++ +P I+THP GH +P    +    +E F  R+   L   E
Sbjct: 203 ALADTFTDPQILTHPGGHFLPAQASQKQTYVEFFRERLQFHLEAQE 242

[Close]

BLAST of CsGy2G021120 vs. ExPASy Swiss-Prot
Match: Q9D7E3 (Esterase OVCA2 OS=Mus musculus OX=10090 GN=Ovca2 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.5e-15
Identity = 65/204 (31.86%), Postives = 90/204 (44.12%), Query Frame = 0


Query: 39  RFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPA------EGKSDVEGIFDP- 98
           R LCL GFR S    +++ G    ++  + +L  L  P P       EG     G   P 
Sbjct: 8   RVLCLAGFRQSERGFREKTGALRKTLRGRAELVCLSGPHPVPEAAAPEGSCPDSGPCSPE 67

Query: 99  --PYFEWFQ------FS--PEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAA 158
             P   WF       FS   E T  R   E L  +   +   GPFDG LGFSQGA L+A 
Sbjct: 68  EQPRGWWFSEEEADVFSALEESTVCRGLQEALETVARALDTLGPFDGLLGFSQGAALAAY 127

Query: 159 LPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMP-- 218
           +      G    + P  +F+I+VSG  F    + E    +P+  PSLH  G+ D ++P  
Sbjct: 128 VCALGQAGD--PRFPLPRFIILVSG--FCPRGLKEPILQSPMSLPSLHVFGDTDRVIPSQ 187

Query: 219 HGKKLLESYIEPTIITHPKGHTIP 224
              +L   ++    +TH  GH IP
Sbjct: 188 ESMQLASRFLGAVTLTHSGGHFIP 207

[Close]

BLAST of CsGy2G021120 vs. NCBI nr
Match: XP_004152614.1 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 513 bits (1322), Expect = 8.68e-184
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0


Query: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60
           MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180
           IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS
Sbjct: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240

Query: 241 SKILNENEE 249
           SKILNENEE
Sbjct: 241 SKILNENEE 249

[Close]

BLAST of CsGy2G021120 vs. NCBI nr
Match: XP_008444882.1 (PREDICTED: dihydrofolate reductase-like [Cucumis melo])

HSP 1 Score: 488 bits (1256), Expect = 1.00e-173
Identity = 237/249 (95.18%), Postives = 241/249 (96.79%), Query Frame = 0


Query: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60
           M  FFPSLSSHQ L+TIPQF FPT+PIMGSDQK FTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180
           I+HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS
Sbjct: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFI RI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240

Query: 241 SKILNENEE 249
           SK+LNE EE
Sbjct: 241 SKMLNETEE 249

[Close]

BLAST of CsGy2G021120 vs. NCBI nr
Match: KAE8652134.1 (hypothetical protein Csa_022239 [Cucumis sativus])

HSP 1 Score: 460 bits (1183), Expect = 5.05e-163
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 28  MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 87
           MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 147
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 207
           KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 208 YIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249
           YIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 222

[Close]

BLAST of CsGy2G021120 vs. NCBI nr
Match: KAA0065116.1 (dihydrofolate reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 447 bits (1149), Expect = 7.71e-158
Identity = 216/222 (97.30%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 28  MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 87
           MGSDQK FTKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 147
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 207
           KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 208 YIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249
           YIEPTIITHPKGHTIPRLDDKALEVMESFI RISK+LNE EE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

[Close]

BLAST of CsGy2G021120 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 429 bits (1102), Expect = 2.00e-150
Identity = 204/225 (90.67%), Postives = 213/225 (94.67%), Query Frame = 0


Query: 25  IPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSD 84
           IPIMG+D K   KPRFLCLHGFRTSAAILKKQVGKWP SVLDQ DLHFLDAPFPAEGKSD
Sbjct: 14  IPIMGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQFDLHFLDAPFPAEGKSD 73

Query: 85  VEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPG 144
           VEGIFDPPYFEWFQFS EFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGAILSAALPG
Sbjct: 74  VEGIFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPG 133

Query: 145 FQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKL 204
           FQAKGIALTKVPKIKFVIIVSGAKFRSESVA+KAYSTPIGCPSLHFLGEEDFLMPHG+KL
Sbjct: 134 FQAKGIALTKVPKIKFVIIVSGAKFRSESVAQKAYSTPIGCPSLHFLGEEDFLMPHGQKL 193

Query: 205 LESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249
           LESY+EPT+I HPKGHT+PRLDDK LEVMESFI RISK L+E EE
Sbjct: 194 LESYVEPTVINHPKGHTVPRLDDKGLEVMESFIKRISKTLDETEE 238

[Close]

BLAST of CsGy2G021120 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 513 bits (1322), Expect = 4.20e-184
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0


Query: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60
           MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180
           IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS
Sbjct: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240

Query: 241 SKILNENEE 249
           SKILNENEE
Sbjct: 241 SKILNENEE 249

[Close]

BLAST of CsGy2G021120 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 4.86e-174
Identity = 237/249 (95.18%), Postives = 241/249 (96.79%), Query Frame = 0


Query: 1   MPPFFPSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKW 60
           M  FFPSLSSHQ L+TIPQF FPT+PIMGSDQK FTKPRFLCLHGFRTSAAILKKQVGKW
Sbjct: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120
           P SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM
Sbjct: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 IKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180
           I+HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS
Sbjct: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRI 240
           TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFI RI
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240

Query: 241 SKILNENEE 249
           SK+LNE EE
Sbjct: 241 SKMLNETEE 249

[Close]

BLAST of CsGy2G021120 vs. ExPASy TrEMBL
Match: A0A5A7VFL2 (Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004510 PE=4 SV=1)

HSP 1 Score: 447 bits (1149), Expect = 3.73e-158
Identity = 216/222 (97.30%), Postives = 218/222 (98.20%), Query Frame = 0


Query: 28  MGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEG 87
           MGSDQK FTKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQA 147
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMI+HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 207
           KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 208 YIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249
           YIEPTIITHPKGHTIPRLDDKALEVMESFI RISK+LNE EE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

[Close]

BLAST of CsGy2G021120 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 3.78e-146
Identity = 202/244 (82.79%), Postives = 219/244 (89.75%), Query Frame = 0


Query: 6   PSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVL 65
           P L S  S T  P+F    IPIMG+D +   KPRFLCLHGFRTS AILKKQVGKWP SVL
Sbjct: 6   PLLCSPISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVL 65

Query: 66  DQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGP 125
           +QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGP
Sbjct: 66  EQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGP 125

Query: 126 FDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGC 185
           FDGFLGFSQGAILSAALPG QAKG+ALTKVPKIKFVIIVSGAKFRS+SVAEKAYS+ IGC
Sbjct: 126 FDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGC 185

Query: 186 PSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILN 245
           PSLHFLG+EDFLMPHG+KLLESYIEP +I HPKGHTIPRLDD+ LEVMESFI +ISK+L+
Sbjct: 186 PSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKISKMLD 245

Query: 246 ENEE 249
           E EE
Sbjct: 246 EAEE 249

[Close]

BLAST of CsGy2G021120 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 416 bits (1069), Expect = 1.54e-145
Identity = 203/244 (83.20%), Postives = 218/244 (89.34%), Query Frame = 0


Query: 6   PSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVL 65
           P L S  S +  P+F    IPIMG+D +   KPRFLCLHGFRTS AILKKQVGKWP SVL
Sbjct: 6   PLLCSPISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVL 65

Query: 66  DQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGP 125
           +QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGP
Sbjct: 66  EQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGP 125

Query: 126 FDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGC 185
           FDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS  IGC
Sbjct: 126 FDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGC 185

Query: 186 PSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILN 245
           PSLHFLG+EDFLMPHG+KLLESYIEP II HPKGHTIPRLDD+ LEVMESFI +ISK+L+
Sbjct: 186 PSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKMLD 245

Query: 246 ENEE 249
           E EE
Sbjct: 246 EAEE 249

[Close]

BLAST of CsGy2G021120 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 304.7 bits (779), Expect = 6.9e-83
Identity = 141/223 (63.23%), Postives = 176/223 (78.92%), Query Frame = 0


Query: 28  MGSD-QKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVE 87
           MGS+ +    KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 88  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQ 147
           GIFDPPY+EWFQF+ EFTEY NF++CL ++E+ MIK GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 148 AKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLE 207
           AKGIA  KVPKIKFVII+ GAK +S  +AE AYS+ +   SLHFLGE DFL P+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 208 SYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 250
           SY  P ++ HPKGHT+PRLD+K+LE + +FI  +  ++ E ++
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDK 223

[Close]

BLAST of CsGy2G021120 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 5.3e-75
Identity = 129/214 (60.28%), Postives = 162/214 (75.70%), Query Frame = 0


Query: 38  PRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWF 97
           PR LCLHGFRTS  IL+  +GKWP ++L  LDL FLDAPFPA GKSDVE  FDPPY+EW+
Sbjct: 30  PRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYEWY 89

Query: 98  QFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPK 157
           Q +  F EYRNF+ECL++IE+YMIK+GPFDG LGFSQGA L+AA+PG Q +G ALTKVPK
Sbjct: 90  QANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKVPK 149

Query: 158 IKFVIIVSGAK-----FRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPT 217
           +KF++I+SGAK     F     A  A+S+P+ CPSLHF+GE DFL   G+ L+ES++EP 
Sbjct: 150 VKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFVEPV 209

Query: 218 IITHPKGHTIPRLDDKALEVMESFIHRISKILNE 247
           +I H  GH IP+LD KA E M SF   I ++L++
Sbjct: 210 VIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

[Close]

BLAST of CsGy2G021120 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 240.7 bits (613), Expect = 1.2e-63
Identity = 114/166 (68.67%), Postives = 134/166 (80.72%), Query Frame = 0


Query: 28  MGSD-QKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVE 87
           MGS+ +    KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 88  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAILSAALPGFQ 147
           GIFDPPY+EWFQF+ EFTEY NF++CL ++E+ MIK GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 148 AKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLG 193
           AKGIA  KVPKIKFVII+ GAK +S  +AE AYS+ +   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

[Close]

BLAST of CsGy2G021120 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 82.8 bits (203), Expect = 4.3e-16
Identity = 68/217 (31.34%), Postives = 97/217 (44.70%), Query Frame = 0


Query: 37  KPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPF----------PAEGKSD-- 96
           K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  +  
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 97  ----VEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIENYMIKHGPFDGFLGFS 156
               V   FD P    +     QF P    T+   FD+ L++++    + GPFDG LGFS
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFS 472

Query: 157 QGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFL-- 216
           QGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH    
Sbjct: 473 QGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIFGS 532

Query: 217 --GEEDFLMPHGKKLLESYIE---PTIITHPKGHTIP 224
             G++  ++      L    E    TI+ H  GH IP
Sbjct: 533 QPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

The following BLAST results are available for this feature:

BLAST of CsGy2G021120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36591	4.0e-19	31.73	Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
Q7QBJ0	6.5e-17	31.16	Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3	[more]
Q3SZ07	1.6e-15	30.88	Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1	[more]
Q0C7C4	2.7e-15	29.65	Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1	[more]
Q9D7E3	3.5e-15	31.86	Esterase OVCA2 OS=Mus musculus OX=10090 GN=Ovca2 PE=1 SV=1	[more]

BLAST of CsGy2G021120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152614.1	8.68e-184	100.00	dihydrofolate reductase [Cucumis sativus]	[more]
XP_008444882.1	1.00e-173	95.18	PREDICTED: dihydrofolate reductase-like [Cucumis melo]	[more]
KAE8652134.1	5.05e-163	100.00	hypothetical protein Csa_022239 [Cucumis sativus]	[more]
KAA0065116.1	7.71e-158	97.30	dihydrofolate reductase-like [Cucumis melo var. makuwa]	[more]
XP_038885360.1	2.00e-150	90.67	dihydrofolate reductase-like [Benincasa hispida]	[more]

BLAST of CsGy2G021120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNX4	4.20e-184	100.00	FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 S...	[more]
A0A1S3BC97	4.86e-174	95.18	dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1	[more]
A0A5A7VFL2	3.73e-158	97.30	Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A6J1K928	3.78e-146	82.79	dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV...	[more]
A0A6J1HF66	1.54e-145	83.20	dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 ...	[more]

BLAST of CsGy2G021120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24380.1	6.9e-83	63.23	INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri...	[more]
AT5G65400.1	5.3e-75	60.28	alpha/beta-Hydrolases superfamily protein	[more]
AT4G24380.2	1.2e-63	68.67	CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidop...	[more]
AT1G09280.1	4.3e-16	31.34	CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005645	Serine hydrolase FSH	PFAM	PF03959	FSH1	coord: 36..227 e-value: 1.3E-47 score: 162.2
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 34..243 e-value: 9.9E-49 score: 168.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 37..223
None	No IPR available	PANTHER	PTHR48070	ESTERASE OVCA2	coord: 35..248
None	No IPR available	PANTHER	PTHR48070:SF5	DIHYDROFOLATE REDUCTASE	coord: 35..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy2G021120.1	CsGy2G021120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004146	dihydrofolate reductase activity