CsGy2G016097 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G016097
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionaspartic proteinase Asp1-like
LocationGy14Chr2: 16073418 .. 16076484 (+)
RNA-Seq ExpressionCsGy2G016097
SyntenyCsGy2G016097
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAAAAGGGTATTGGTGGTACTGGTTCTGATGGTGGCCTCCATGAGTTGTTTGGCTCCGTGTTCAGCTTCGTCGTTTTTTAAGGATAAGCCATGGGAGCGAAAGAGGCCAATCCTGTCGGTTCCGACTGCATCTTCTTCGTTTGCTTCATCCTCCATTGTATTGCCTCTTCAAGGGAACGTCTATCCAAATGGGTAAGCTATAACTCTGCTCAAAGATACGACGATGTTCATCTTTTGTTTCCCCCTTTCAATTTTACTCTACTGTTATATAGTACAAAGTCCAACTCCCAATATACTTTTCACATTGATGTATTGTAATAATCTCAGTTGTCCTTCCCCATTATTGACCTTTTATTGTATCTTCGCTTGCGTGTGAAAATTAGAGAAAAGATGATTACGAAACTGTGTTCTCTCATGTTTGCTTTTGAGGAGTGTCTTTATGAGTGATTTTTTTCTCAACTTTGTAGCAAAATATAACATCCCCATATCAAATACTTTTAAAATTTTCATACCCATTTTACCGAATGTTTTTATTTACAAACAGTTTGAAAAATATATATATATATATATTTCCTTGTTCTCGTCGCAGGCTAACTTTTTTACTGTCAGATCGTAAATACCATTTCATCAATATGTTTTGAGGTTACTATAATCCGTGTCTTTTTTCAGGTTCTATAACGTTACTCTTTATGTAGGACAGCCTCCAAAGCCTTACTTTCTAGATCCCGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAACAGTGCACTGAGGTAAACTTTTCTTTGACGCAAATTTGGAGATTCTTTTAAATATGATGAACTAAACGATGTAACATAGTTATTAAGATACAGCTTTGGCTTCAGTTAAATTAATTTTGATCACAAGTAACCCAAAATTAGTTAATTTTGATCATAAGTAACCCGAAATTTTATTCTTGAATGGTCTATTTTGTTCTTTGTTCCCGCTATATTTCTTCTTGGTGTGCTTAGTGTTTTTTTTCCTTCTTTCCCTCAGTCTCTCACTACCCTTTGCATTTCTTTTGGGGTTGAGAATTAAGGTTGACTGCTTCAAACTGATGGCCTTGTTTCCTTTACAGACACTTCATCCACTTTATCAACCAAGCAACGATCTTGTCCCGTGTAAGGACCCTCTGTGCATGTCCTTGCACTCATCTATGGACCACAGATGCGAAAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCATCTCTTGGAGTCCTTGTCAGGGATGTATTTCCTCTCAACTTAACCAATGGAGACCCAATTAGACCCCGTCTGGCCCTCGGGTAAACTGCTCGACATCCTTCAGTAATTCATCTCAATTCAGTGTACTGATGAGTTCCTAGCTCTCTAAATCAGTCTTGCTACTTCCAATTACAATGTCACACGCGAGTCTTGAAAAAGAAACATCATATAATGCTGTCATATCTTATGTTTTGCACTTTCCTGATATTGCAAGGTAATGATAGCATGAATGTTAAACTAGAGTATATATTGAATAACAAACGTGTGCATTCATGTGATGGGTTACATCAAGAAGACACTTAATTAAGATCGCAACGGGCACTAGAATTTGTGATGGGCATAATGATTCTGATTTGTAACTTTTATAGTCATCAAGAAAGAACATCTTGGACCAGAGATTGATTCACTTTGCTAATTTCTCATCCTTCCGAACTGCAGTTGTCAGCTCATGTGGGTAAATAGCATGCATGTGTTTTTAGCTATTTGAATCTGGAGTGCTAAGTTAGTTTTCATTTTATTACTCAGATGTGGTTATGATCAAGATCCTGGATCGTCATCTTATCACCCCATGGATGGAATACTGGGCCTTGGAAGAGGAGCAGTAAGCATCGTCTCACAACTGCATAACCAAGGCATTGTCCGTAACGTCGTTGGTCACTGTTTCAACAGCAAAGGAGGAGGATATCTTTTCTTTGGGGATGGCATATATGATCCCTATCGTTTAGTTTGGACGCCCATGTCACGGGACTACCCGTAAACAACATGCCTTGATCATATATATATATATATATGTTATTTTAATGATTCATGTTAGCTTCTGTTTTTATAGAACCTGCCCCTTATGTGACTATCAGGAAGCACTACTCCCCTGGGTTTGGAGAACTAATTTTCAATGGAAGAAGTACTGGACTCAGAAATTTGTTTGTAGTTTTTGACAGTGGGAGCTCTTACACATACTTCAATGCTCAGGCTTATCAAGTTTTAACATCATTGGTAAATCATCTGTAAAGGAAACTTGACTGCCTTGGAAGATTAGATCTTTCTCTCTCATGAAAACTTTGGGAATACGCAGTGATTCTTCAATTCATGTGGCACTGTTTTTGTAGTTGAATAGAGAACTAGCTGGAAAACCGCTAAGAGAAGCCATGGATGATGATACGCTTCCGCTCTGCTGGAGAGGGCGGAAGCCAATCAAAAGCTTACGTGATGTGAGAAAATATTTCAAGCCATTGGCCTTGAGCTTTTCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAACGGAAGGTTACATGATAATATCGGTAAAAGCTCCATATCTTCAACTTCAACTGCTATTAACTTGTTTTCTTTGGATTTTCAAGTTGAACGACTTCAATTATATAAGATAACTTTGGATTTTCAAGTTGAACGACTTCAATTATATAAGATAACTTTGGAATGGCAGTCCATGGGAAATGTTTGCTTGGGAATTCTGAACGGCACCGACGTTGGTCTTGAAAACTCGAATATCATCGGTGGTACGTTATTTTAATTTATTGCATGCACATGTTATATTATTTTCTCGCAATGACTTCGAAACCATGCAAGAATATTCTTTTATTAGAGAGAGAAAAAAAAGAATCCCCATCTTCTGTATGCTTTTGTTGACAGATATTTCGATGCAAGATAAAATGGTAGTATACAACAATGAGAAGCAAGCAATTGGATGGGCTACTGCCAACTGTGATCGGGTTCCCAAGTCTCAAGTCTCGAGTTGGTAG

mRNA sequence

ATGGGCAAAAGGGTATTGGTGGTACTGGTTCTGATGGTGGCCTCCATGAGTTGTTTGGCTCCGTGTTCAGCTTCGTCGTTTTTTAAGGATAAGCCATGGGAGCGAAAGAGGCCAATCCTGTCGGTTCCGACTGCATCTTCTTCGTTTGCTTCATCCTCCATTGTATTGCCTCTTCAAGGGAACGTCTATCCAAATGGGTTCTATAACGTTACTCTTTATGTAGGACAGCCTCCAAAGCCTTACTTTCTAGATCCCGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAACAGTGCACTGAGACACTTCATCCACTTTATCAACCAAGCAACGATCTTGTCCCGTGTAAGGACCCTCTGTGCATGTCCTTGCACTCATCTATGGACCACAGATGCGAAAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCATCTCTTGGAGTCCTTGTCAGGGATGTATTTCCTCTCAACTTAACCAATGGAGACCCAATTAGACCCCGTCTGGCCCTCGGATGTGGTTATGATCAAGATCCTGGATCGTCATCTTATCACCCCATGGATGGAATACTGGGCCTTGGAAGAGGAGCAGTAAGCATCGTCTCACAACTGCATAACCAAGGCATTGTCCGTAACGTCGTTGGTCACTGTTTCAACAGCAAAGGAGGAGGATATCTTTTCTTTGGGGATGGCATATATGATCCCTATCGTTTAGTTTGGACGCCCATGTCACGGGACTACCCGAAGCACTACTCCCCTGGGTTTGGAGAACTAATTTTCAATGGAAGAAGTACTGGACTCAGAAATTTGTTTGTAGTTTTTGACAGTGGGAGCTCTTACACATACTTCAATGCTCAGGCTTATCAAGTTTTAACATCATTGTTGAATAGAGAACTAGCTGGAAAACCGCTAAGAGAAGCCATGGATGATGATACGCTTCCGCTCTGCTGGAGAGGGCGGAAGCCAATCAAAAGCTTACGTGATGTGAGAAAATATTTCAAGCCATTGGCCTTGAGCTTTTCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAACGGAAGGTTACATGATAATATCGTCCATGGGAAATGTTTGCTTGGGAATTCTGAACGGCACCGACGTTGGTCTTGAAAACTCGAATATCATCGGTGATATTTCGATGCAAGATAAAATGGTAGTATACAACAATGAGAAGCAAGCAATTGGATGGGCTACTGCCAACTGTGATCGGGTTCCCAAGTCTCAAGTCTCGAGTTGGTAG

Coding sequence (CDS)

ATGGGCAAAAGGGTATTGGTGGTACTGGTTCTGATGGTGGCCTCCATGAGTTGTTTGGCTCCGTGTTCAGCTTCGTCGTTTTTTAAGGATAAGCCATGGGAGCGAAAGAGGCCAATCCTGTCGGTTCCGACTGCATCTTCTTCGTTTGCTTCATCCTCCATTGTATTGCCTCTTCAAGGGAACGTCTATCCAAATGGGTTCTATAACGTTACTCTTTATGTAGGACAGCCTCCAAAGCCTTACTTTCTAGATCCCGACACCGGTAGTGATCTCACTTGGCTTCAATGTGACGCTCCATGTCAACAGTGCACTGAGACACTTCATCCACTTTATCAACCAAGCAACGATCTTGTCCCGTGTAAGGACCCTCTGTGCATGTCCTTGCACTCATCTATGGACCACAGATGCGAAAACCCAGATCAATGTGACTACGAGGTTGAGTATGCAGATGGTGGTTCATCTCTTGGAGTCCTTGTCAGGGATGTATTTCCTCTCAACTTAACCAATGGAGACCCAATTAGACCCCGTCTGGCCCTCGGATGTGGTTATGATCAAGATCCTGGATCGTCATCTTATCACCCCATGGATGGAATACTGGGCCTTGGAAGAGGAGCAGTAAGCATCGTCTCACAACTGCATAACCAAGGCATTGTCCGTAACGTCGTTGGTCACTGTTTCAACAGCAAAGGAGGAGGATATCTTTTCTTTGGGGATGGCATATATGATCCCTATCGTTTAGTTTGGACGCCCATGTCACGGGACTACCCGAAGCACTACTCCCCTGGGTTTGGAGAACTAATTTTCAATGGAAGAAGTACTGGACTCAGAAATTTGTTTGTAGTTTTTGACAGTGGGAGCTCTTACACATACTTCAATGCTCAGGCTTATCAAGTTTTAACATCATTGTTGAATAGAGAACTAGCTGGAAAACCGCTAAGAGAAGCCATGGATGATGATACGCTTCCGCTCTGCTGGAGAGGGCGGAAGCCAATCAAAAGCTTACGTGATGTGAGAAAATATTTCAAGCCATTGGCCTTGAGCTTTTCCAGTGGTGGAAGAAGCAAAGCAGTGTTTGAAATACCAACGGAAGGTTACATGATAATATCGTCCATGGGAAATGTTTGCTTGGGAATTCTGAACGGCACCGACGTTGGTCTTGAAAACTCGAATATCATCGGTGATATTTCGATGCAAGATAAAATGGTAGTATACAACAATGAGAAGCAAGCAATTGGATGGGCTACTGCCAACTGTGATCGGGTTCCCAAGTCTCAAGTCTCGAGTTGGTAG

Protein sequence

MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDRVPKSQVSSW*
Homology
BLAST of CsGy2G016097 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 4.3e-93
Identity = 183/389 (47.04%), Postives = 256/389 (65.81%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++VL L GNVYP G + +T+ +G P K YFLD DTGS LTWLQCDAPC  C    H L
Sbjct: 21  SSAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVL 80

Query: 111 YQPS-NDLVPCKDPLCMSLHSSM--DHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNL 170
           Y+P+   LV C D LC  L++ +    RC +  QCDY ++Y D  SS+GVLV D F L+ 
Sbjct: 81  YKPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSA 140

Query: 171 TNGDPIRPRLALGCGYDQDPGSSSYH-PMDGILGLGRGAVSIVSQLHNQGIV-RNVVGHC 230
           +NG      +A GCGYDQ   + +   P+D ILGL RG V+++SQL +QG++ ++V+GHC
Sbjct: 141 SNGTN-PTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 231 FNSKGGGYLFFGDGIYDPYRLVWTPMSRDYPKHYSPGFGELIF--NGRSTGLRNLFVVFD 290
            +SKGGG+LFFGD       + WTPM+R++ K+YSPG G L F  N ++     + V+FD
Sbjct: 201 ISSKGGGFLFFGDAQVPTSGVTWTPMNREH-KYYSPGHGTLHFDSNSKAISAAPMAVIFD 260

Query: 291 SGSSYTYFNAQAYQ----VLTSLLNRELAGKPLREAMDDD-TLPLCWRGRKPIKSLRDVR 350
           SG++YTYF AQ YQ    V+ S LN E   K L E  + D  L +CW+G+  I ++ +V+
Sbjct: 261 SGATYTYFAAQPYQATLSVVKSTLNSEC--KFLTEVTEKDRALTVCWKGKDKIVTIDEVK 320

Query: 351 KYFKPLALSFSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGT--DVGLENSNIIGDIS 410
           K F+ L+L F+ G + KA  EIP E Y+IIS  G+VCLGIL+G+   + L  +N+IG I+
Sbjct: 321 KCFRSLSLEFADGDK-KATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGIT 380

Query: 411 MQDKMVVYNNEKQAIGWATANCDRVPKSQ 426
           M D+MV+Y++E+  +GW    CDR+P+S+
Sbjct: 381 MLDQMVIYDSERSLLGWVNYQCDRIPRSE 403

BLAST of CsGy2G016097 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 326.6 bits (836), Expect = 4.1e-88
Identity = 173/386 (44.82%), Postives = 249/386 (64.51%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++VL L GNVYP G + VT+ +G P KPYFLD DTGS LTWLQCD PC  C +  H L
Sbjct: 21  SSAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGL 80

Query: 111 YQPS-NDLVPCKDPLCMSLHSSM--DHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNL 170
           Y+P     V C +  C  L++ +    +C   +QC Y ++Y  GGSS+GVL+ D F L  
Sbjct: 81  YKPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYV-GGSSIGVLIVDSFSLPA 140

Query: 171 TNGDPIRPRLALGCGYDQDPGSSSY-HPMDGILGLGRGAVSIVSQLHNQGIV-RNVVGHC 230
           +NG      +A GCGY+Q   + +   P++GILGLGRG V+++SQL +QG++ ++V+GHC
Sbjct: 141 SNGTN-PTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 231 FNSKGGGYLFFGDGIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGL--RNLFVVFD 290
            +SKG G+LFFGD       + W+PM+R++ KHYSP  G L FN  S  +    + V+FD
Sbjct: 201 ISSKGKGFLFFGDAKVPTSGVTWSPMNREH-KHYSPRQGTLQFNSNSKPISAAPMEVIFD 260

Query: 291 SGSSYTYFNAQAYQVLTSLLNRELAG--KPLREAMDDD-TLPLCWRGRKPIKSLRDVRKY 350
           SG++YTYF  Q Y    S++   L+   K L E  + D  L +CW+G+  I+++ +V+K 
Sbjct: 261 SGATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKC 320

Query: 351 FKPLALSFSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGT--DVGLENSNIIGDISMQ 410
           F+ L+L F+ G + KA  EIP E Y+IIS  G+VCLGIL+G+     L  +N+IG I+M 
Sbjct: 321 FRSLSLKFADGDK-KATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITML 380

Query: 411 DKMVVYNNEKQAIGWATANCDRVPKS 425
           D+MV+Y++E+  +GW    CDR+P+S
Sbjct: 381 DQMVIYDSERSLLGWVNYQCDRIPRS 402

BLAST of CsGy2G016097 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 5.4e-80
Identity = 170/397 (42.82%), Postives = 237/397 (59.70%), Query Frame = 0

Query: 44  TASSSFASSSIVLPLQGNVYPNGFYNVTLYVGQPP--KPYFLDPDTGSDLTWLQCDAPCQ 103
           T++ S  SS+ + P+ GNVYP+G Y   + VG+P   + Y LD DTGS+LTW+QCDAPC 
Sbjct: 179 TSAGSIDSSTTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCT 238

Query: 104 QCTETLHPLYQPSND-LVPCKDPLCMSL-HSSMDHRCENPDQCDYEVEYADGGSSLGVLV 163
            C +  + LY+P  D LV   +  C+ +  + +   CEN  QCDYE+EYAD   S+GVL 
Sbjct: 239 SCAKGANQLYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCHQCDYEIEYADHSYSMGVLT 298

Query: 164 RDVFPLNLTNGDPIRPRLALGCGYDQDP-GSSSYHPMDGILGLGRGAVSIVSQLHNQGIV 223
           +D F L L NG      +  GCGYDQ     ++    DGILGL R  +S+ SQL ++GI+
Sbjct: 299 KDKFHLKLHNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGII 358

Query: 224 RNVVGHCFNS--KGGGYLFFGDGIYDPYRLVWTPMSRD--------YPKHYSPGFGELIF 283
            NVVGHC  S   G GY+F G  +   + + W PM  D             S G G L  
Sbjct: 359 SNVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSL 418

Query: 284 NGRSTGLRNLFVVFDSGSSYTYFNAQAY-QVLTSLLNRELAGKPLREAMDDDTLPLCWRG 343
           +G +  +    V+FD+GSSYTYF  QAY Q++TSL  +E++G  L     D+TLP+CWR 
Sbjct: 419 DGENGRVGK--VLFDTGSSYTYFPNQAYSQLVTSL--QEVSGLELTRDDSDETLPICWRA 478

Query: 344 RK--PIKSLRDVRKYFKPLALSFSSGGR--SKAVFEIPTEGYMIISSMGNVCLGILNGTD 403
           +   P  SL DV+K+F+P+ L   S     S+ +  I  E Y+IIS+ GNVCLGIL+G+ 
Sbjct: 479 KTNFPFSSLSDVKKFFRPITLQIGSKWLIISRKLL-IQPEDYLIISNKGNVCLGILDGSS 538

Query: 404 VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 421
           V   ++ I+GDISM+  ++VY+N K+ IGW  ++C R
Sbjct: 539 VHDGSTIILGDISMRGHLIVYDNVKRRIGWMKSDCVR 570

BLAST of CsGy2G016097 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 3.8e-33
Identity = 124/438 (28.31%), Postives = 190/438 (43.38%), Query Frame = 0

Query: 5   VLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQGN--V 64
           V+ V V+++   S      A   F  K  +      S  T   S   +SI LPL G+  V
Sbjct: 10  VVAVFVIVIEFASANFVFKAQHKFAGKK-KNLEHFKSHDTRRHSRMLASIDLPLGGDSRV 69

Query: 65  YPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPS-------- 124
              G Y   + +G PPK Y +  DTGSD+ W+ C  PC +C    +  ++ S        
Sbjct: 70  DSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPTKTNLNFRLSLFDMNASS 129

Query: 125 -NDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGD-- 184
            +  V C D  C  +  S    C+    C Y + YAD  +S G  +RD+  L    GD  
Sbjct: 130 TSKKVGCDDDFCSFI--SQSDSCQPALGCSYHIVYADESTSDGKFIRDMLTLEQVTGDLK 189

Query: 185 --PIRPRLALGCGYDQDPG-SSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCF-N 244
             P+   +  GCG DQ     +    +DG++G G+   S++SQL   G  + V  HC  N
Sbjct: 190 TGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDN 249

Query: 245 SKGGGYLFFGDGIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGL-----RNLFVVF 304
            KGGG   F  G+ D  ++  TPM  +   HY+     +  +G S  L     RN   + 
Sbjct: 250 VKGGG--IFAVGVVDSPKVKTTPMVPN-QMHYNVMLMGMDVDGTSLDLPRSIVRNGGTIV 309

Query: 305 DSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFK 364
           DSG++  YF    Y    SL+   LA +P++  + ++T        +      +V + F 
Sbjct: 310 DSGTTLAYFPKVLYD---SLIETILARQPVKLHIVEETF-------QCFSFSTNVDEAFP 369

Query: 365 PLALSFSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNII--GDISMQDK 419
           P++  F    +      +    Y+        C G   G     E S +I  GD+ + +K
Sbjct: 370 PVSFEFEDSVK----LTVYPHDYLFTLEEELYCFGWQAGGLTTDERSEVILLGDLVLSNK 426

BLAST of CsGy2G016097 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.3e-28
Identity = 114/375 (30.40%), Postives = 164/375 (43.73%), Query Frame = 0

Query: 67  FYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP---------LYQP---- 126
           + NVT  VG P   + +  DTGSDL WL CD  C  C   L           +Y P    
Sbjct: 105 YANVT--VGTPSDWFMVALDTGSDLFWLPCD--CTNCVRELKAPGGSSLDLNIYSPNASS 164

Query: 127 SNDLVPCKDPLCMSLHSSMDHRCENPD-QCDYEVEY-ADGGSSLGVLVRDVFPL--NLTN 186
           ++  VPC   LC     +   RC +P+  C Y++ Y ++G SS GVLV DV  L  N  +
Sbjct: 165 TSTKVPCNSTLC-----TRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSNDKS 224

Query: 187 GDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSK 246
              I  R+  GCG  Q          +G+ GLG   +S+ S L  +GI  N    CF + 
Sbjct: 225 SKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFGND 284

Query: 247 GGGYLFFGD-GIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSY 306
           G G + FGD G  D      TP++   P          I  G +TG      VFDSG+S+
Sbjct: 285 GAGRISFGDKGSVDQRE---TPLNIRQPHPTYNITVTKISVGGNTGDLEFDAVFDSGTSF 344

Query: 307 TYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPL--CWRGRKPIKSLRDVRKYFKPLAL 366
           TY    AY +++   N     K  R    D  LP   C+       +L   +  F+  A+
Sbjct: 345 TYLTDAAYTLISESFNSLALDK--RYQTTDSELPFEYCY-------ALSPNKDSFQYPAV 404

Query: 367 SFSSGGRSKAVFEIPTEGYMIISSMGNV---CLGILNGTDVGLENSNIIGDISMQDKMVV 419
           + +  G S      P    +++  M +    CL I+      +E+ +IIG   M    VV
Sbjct: 405 NLTMKGGS----SYPVYHPLVVIPMKDTDVYCLAIMK-----IEDISIIGQNFMTGYRVV 449

BLAST of CsGy2G016097 vs. NCBI nr
Match: XP_004147327.2 (aspartic proteinase Asp1 isoform X1 [Cucumis sativus] >KAE8651999.1 hypothetical protein Csa_016941 [Cucumis sativus])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSSW 429
           VPKSQVSSW
Sbjct: 421 VPKSQVSSW 429

BLAST of CsGy2G016097 vs. NCBI nr
Match: XP_008460823.1 (PREDICTED: aspartic proteinase Asp1 isoform X2 [Cucumis melo])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 424/429 (98.83%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSSW 429
           VPKSQVSSW
Sbjct: 421 VPKSQVSSW 429

BLAST of CsGy2G016097 vs. NCBI nr
Match: TYK02025.1 (aspartic proteinase Asp1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 424/429 (98.83%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 42  MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 101

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 102 NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 161

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 162 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 221

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 222 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 281

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 282 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 341

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 342 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 401

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 402 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 461

Query: 421 VPKSQVSSW 429
           VPKSQVSSW
Sbjct: 462 VPKSQVSSW 470

BLAST of CsGy2G016097 vs. NCBI nr
Match: XP_008460822.1 (PREDICTED: aspartic proteinase Asp1 isoform X1 [Cucumis melo])

HSP 1 Score: 876 bits (2263), Expect = 0.0
Identity = 424/433 (97.92%), Postives = 425/433 (98.15%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 C----GYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240
           C    GYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF
Sbjct: 181 CQLICGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240

Query: 241 GDGIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300
           GDGIYDPYRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAY
Sbjct: 241 GDGIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300

Query: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKA 360
           QVLTSLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKA
Sbjct: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA 360

Query: 361 VFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420
           VFEIP EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA
Sbjct: 361 VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420

Query: 421 NCDRVPKSQVSSW 429
           NCDRVPKSQVSSW
Sbjct: 421 NCDRVPKSQVSSW 433

BLAST of CsGy2G016097 vs. NCBI nr
Match: XP_038900559.1 (aspartic proteinase Asp1 isoform X2 [Benincasa hispida])

HSP 1 Score: 866 bits (2237), Expect = 0.0
Identity = 412/428 (96.26%), Postives = 422/428 (98.60%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           M KRVL++LVLMVASMSCLAPCSASSFFKDKPWER+RPILSVP ASSSFASSSIV+PLQG
Sbjct: 1   MEKRVLMILVLMVASMSCLAPCSASSFFKDKPWERRRPILSVPIASSSFASSSIVMPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDG+LGLGRGAVS+VSQLHNQGIVRNVVGHCF+SKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGVLGLGRGAVSMVSQLHNQGIVRNVVGHCFSSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYR+VWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRIVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRGRKP KSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGY+IISSMGN CLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PMEGYLIISSMGNACLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSS 428
           VPKS+V S
Sbjct: 421 VPKSRVGS 428

BLAST of CsGy2G016097 vs. ExPASy TrEMBL
Match: A0A5D3BS69 (Aspartic proteinase Asp1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00070 PE=4 SV=1)

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 424/429 (98.83%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 42  MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 101

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 102 NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 161

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 162 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 221

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 222 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 281

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 282 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 341

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 342 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 401

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 402 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 461

Query: 421 VPKSQVSSW 429
           VPKSQVSSW
Sbjct: 462 VPKSQVSSW 470

BLAST of CsGy2G016097 vs. ExPASy TrEMBL
Match: A0A1S3CDB2 (aspartic proteinase Asp1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4 SV=1)

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 424/429 (98.83%), Postives = 425/429 (99.07%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI
Sbjct: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YDPYRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR
Sbjct: 361 PIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSSW 429
           VPKSQVSSW
Sbjct: 421 VPKSQVSSW 429

BLAST of CsGy2G016097 vs. ExPASy TrEMBL
Match: A0A1S3CDB4 (aspartic proteinase Asp1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4 SV=1)

HSP 1 Score: 876 bits (2263), Expect = 0.0
Identity = 424/433 (97.92%), Postives = 425/433 (98.15%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MGK VLVVL LMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG
Sbjct: 1   MGKWVLVVLALMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180

Query: 181 C----GYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240
           C    GYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF
Sbjct: 181 CQLICGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFF 240

Query: 241 GDGIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300
           GDGIYDPYRLVWTPMSRDYPKHYSPGFGEL+FNGRSTGLRNLFVVFDSGSSYTYFNAQAY
Sbjct: 241 GDGIYDPYRLVWTPMSRDYPKHYSPGFGELMFNGRSTGLRNLFVVFDSGSSYTYFNAQAY 300

Query: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKA 360
           QVLTSLLNRELAGKPLREAMDDDTLPLCWR RKPIKSLRDVRKYFKPLALSFSSGGRSKA
Sbjct: 301 QVLTSLLNRELAGKPLREAMDDDTLPLCWRERKPIKSLRDVRKYFKPLALSFSSGGRSKA 360

Query: 361 VFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420
           VFEIP EGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA
Sbjct: 361 VFEIPIEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATA 420

Query: 421 NCDRVPKSQVSSW 429
           NCDRVPKSQVSSW
Sbjct: 421 NCDRVPKSQVSSW 433

BLAST of CsGy2G016097 vs. ExPASy TrEMBL
Match: A0A6J1I590 (aspartic proteinase Asp1-like OS=Cucurbita maxima OX=3661 GN=LOC111469733 PE=4 SV=1)

HSP 1 Score: 817 bits (2110), Expect = 4.20e-298
Identity = 387/430 (90.00%), Postives = 409/430 (95.12%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVP--TASSSFASSSIVLPL 60
           MGK VL++LVLMV+S+SCLAPCSASSFFKDK WER+RP LSVP  +ASSS AS SIVLPL
Sbjct: 1   MGKGVLMILVLMVSSISCLAPCSASSFFKDKLWERRRPTLSVPIASASSSIASPSIVLPL 60

Query: 61  QGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLV 120
           QGNV+PNGFYNVTL++GQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLYQPSNDLV
Sbjct: 61  QGNVFPNGFYNVTLFIGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYQPSNDLV 120

Query: 121 PCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLA 180
           PCKDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRL 
Sbjct: 121 PCKDPLCMSLHSSIDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLT 180

Query: 181 LGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGD 240
           LGCGYDQ+PGSSSYHPMDG+LGLG+GAVSIVSQLHNQGIVRNVVGHCF+SKGGGYLFFGD
Sbjct: 181 LGCGYDQNPGSSSYHPMDGVLGLGKGAVSIVSQLHNQGIVRNVVGHCFSSKGGGYLFFGD 240

Query: 241 GIYDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQV 300
            IYDPYRL WTPMSRDYPKHYSPGFG+L FNGRSTGLRNLFVVFDSGSSYTYFNAQAYQ+
Sbjct: 241 DIYDPYRLAWTPMSRDYPKHYSPGFGDLFFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQI 300

Query: 301 LTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVF 360
           +TSLLNREL GKPLREA DDDTLPLCWRGR P KSLRDVRKYFKPLALSFSSG RSKAVF
Sbjct: 301 ITSLLNRELTGKPLREAKDDDTLPLCWRGRNPFKSLRDVRKYFKPLALSFSSGRRSKAVF 360

Query: 361 EIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420
           E+PTE Y+IISS GNVCLGILNG++VGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC
Sbjct: 361 EMPTESYLIISSKGNVCLGILNGSEVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANC 420

Query: 421 DRVPKSQVSS 428
           DRVPKS V S
Sbjct: 421 DRVPKSSVGS 430

BLAST of CsGy2G016097 vs. ExPASy TrEMBL
Match: A0A6J1DZ15 (aspartic proteinase Asp1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024375 PE=4 SV=1)

HSP 1 Score: 816 bits (2109), Expect = 5.74e-298
Identity = 385/428 (89.95%), Postives = 410/428 (95.79%), Query Frame = 0

Query: 1   MGKRVLVVLVLMVASMSCLAPCSASSFFKDKPWERKRPILSVPTASSSFASSSIVLPLQG 60
           MG  +L +LVLMVASM+CLAP SASSFFKDKPWER++PILSV   SSSFASSSIVLPLQG
Sbjct: 1   MGTGLLKILVLMVASMNCLAPSSASSFFKDKPWERRKPILSVSATSSSFASSSIVLPLQG 60

Query: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPLYQPSNDLVPC 120
           NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTET HPLY+PS+DLVPC
Sbjct: 61  NVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETPHPLYRPSDDLVPC 120

Query: 121 KDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTNGDPIRPRLALG 180
           KDPLCMSLHSS+DHRCENPDQCDYEVEYADGGSSLGVLVRD+FPLNLTNGDPIRPRLALG
Sbjct: 121 KDPLCMSLHSSVDHRCENPDQCDYEVEYADGGSSLGVLVRDIFPLNLTNGDPIRPRLALG 180

Query: 181 CGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSKGGGYLFFGDGI 240
           CGYDQ PGSS YHPMDGILGLG+GAVSIVSQLHNQGI+RNV+GHCF+S+GGGYLFFGD I
Sbjct: 181 CGYDQIPGSSYYHPMDGILGLGKGAVSIVSQLHNQGIIRNVIGHCFSSRGGGYLFFGDDI 240

Query: 241 YDPYRLVWTPMSRDYPKHYSPGFGELIFNGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLT 300
           YD +R+VWTPMSRDYPKHYSPG GELIFNGRSTGLRNLF VFDSGSSYTYFNAQAYQVLT
Sbjct: 241 YDSHRVVWTPMSRDYPKHYSPGLGELIFNGRSTGLRNLFAVFDSGSSYTYFNAQAYQVLT 300

Query: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360
           SLLNRELAGKPLREAMDDDTLPLCWRGRKP KSLRDVRKYFKPLALSFSSGGRSKAVFEI
Sbjct: 301 SLLNRELAGKPLREAMDDDTLPLCWRGRKPFKSLRDVRKYFKPLALSFSSGGRSKAVFEI 360

Query: 361 PTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEKQAIGWATANCDR 420
           P EGY+I+SSMGNVCLGILNGT+VGL+NSNIIGDISM DK+V+YNNEKQAIGWATANCDR
Sbjct: 361 PMEGYLILSSMGNVCLGILNGTEVGLQNSNIIGDISMHDKIVIYNNEKQAIGWATANCDR 420

Query: 421 VPKSQVSS 428
           VPKS+ ++
Sbjct: 421 VPKSRAAA 428

BLAST of CsGy2G016097 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.1e-165
Identity = 259/373 (69.44%), Postives = 314/373 (84.18%), Query Frame = 0

Query: 50  ASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP 109
           A SS+V P+ GNVYP G+YNVT+ +GQPP+PY+LD DTGSDLTWLQCDAPC +C E  HP
Sbjct: 42  AVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHP 101

Query: 110 LYQPSNDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTN 169
           LYQPS+DL+PC DPLC +LH + + RCE P+QCDYEVEYADGGSSLGVLVRDVF +N T 
Sbjct: 102 LYQPSSDLIPCNDPLCKALHLNSNQRCETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQ 161

Query: 170 GDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSK 229
           G  + PRLALGCGYDQ PG+SS+HP+DG+LGLGRG VSI+SQLH+QG V+NV+GHC +S 
Sbjct: 162 GLRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSL 221

Query: 230 GGGYLFFGDGIYDPYRLVWTPMSRDYPKHYSPGF-GELIFNGRSTGLRNLFVVFDSGSSY 289
           GGG LFFGD +YD  R+ WTPMSR+Y KHYSP   GEL+F GR+TGL+NL  VFDSGSSY
Sbjct: 222 GGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSY 281

Query: 290 TYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSF 349
           TYFN++AYQ +T LL REL+GKPL+EA DD TLPLCW+GR+P  S+ +V+KYFKPLALSF
Sbjct: 282 TYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSF 341

Query: 350 SSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNEK 409
            +G RSK +FEIP E Y+IIS  GNVCLGILNGT++GL+N N+IGDISMQD+M++Y+NEK
Sbjct: 342 KTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEK 401

Query: 410 QAIGWATANCDRV 422
           Q+IGW   +CD +
Sbjct: 402 QSIGWMPVDCDEL 414

BLAST of CsGy2G016097 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 538.1 bits (1385), Expect = 6.4e-153
Identity = 242/345 (70.14%), Postives = 290/345 (84.06%), Query Frame = 0

Query: 50  ASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHP 109
           A SS+V P+ GNVYP G+YNVT+ +GQPP+PY+LD DTGSDLTWLQCDAPC +C E  HP
Sbjct: 39  AVSSVVFPVHGNVYPLGYYNVTINIGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHP 98

Query: 110 LYQPSNDLVPCKDPLCMSLHSSMDHRCENPDQCDYEVEYADGGSSLGVLVRDVFPLNLTN 169
           LYQPS+DL+PC DPLC +LH + + RCE P+QCDYEVEYADGGSSLGVLVRDVF +N T 
Sbjct: 99  LYQPSSDLIPCNDPLCKALHLNSNQRCETPEQCDYEVEYADGGSSLGVLVRDVFSMNYTQ 158

Query: 170 GDPIRPRLALGCGYDQDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNSK 229
           G  + PRLALGCGYDQ PG+SS+HP+DG+LGLGRG VSI+SQLH+QG V+NV+GHC +S 
Sbjct: 159 GLRLTPRLALGCGYDQIPGASSHHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSL 218

Query: 230 GGGYLFFGDGIYDPYRLVWTPMSRDYPKHYSPGF-GELIFNGRSTGLRNLFVVFDSGSSY 289
           GGG LFFGD +YD  R+ WTPMSR+Y KHYSP   GEL+F GR+TGL+NL  VFDSGSSY
Sbjct: 219 GGGILFFGDDLYDSSRVSWTPMSREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSY 278

Query: 290 TYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALSF 349
           TYFN++AYQ +T LL REL+GKPL+EA DD TLPLCW+GR+P  S+ +V+KYFKPLALSF
Sbjct: 279 TYFNSKAYQAVTYLLKRELSGKPLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSF 338

Query: 350 SSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIG 394
            +G RSK +FEIP E Y+IIS  GNVCLGILNGT++GL+N N+IG
Sbjct: 339 KTGWRSKTLFEIPPEAYLIISMKGNVCLGILNGTEIGLQNLNLIG 383

BLAST of CsGy2G016097 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 9.3e-120
Identity = 196/396 (49.49%), Postives = 284/396 (71.72%), Query Frame = 0

Query: 39  ILSVPTASSSF-------ASSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDL 98
           ++ VP + SS        + SS+V PL GNV+P G+Y+V + +G PPK +  D DTGSDL
Sbjct: 13  LVIVPLSKSSIFKTFIKSSPSSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDL 72

Query: 99  TWLQCDAPCQQCTETLHPLYQPSNDLVPCKDPLCMSLHSSMDHRCENP-DQCDYEVEYAD 158
           TW+QCDAPC  CT   +  Y+P  +++PC +P+C +LH      C NP +QCDYEV+YAD
Sbjct: 73  TWVQCDAPCSGCTLPPNLQYKPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYAD 132

Query: 159 GGSSLGVLVRDVFPLNLTNGDPIRPRLALGCGYDQDPGSSSYHPMD-GILGLGRGAVSIV 218
            GSS+G LV D FPL L NG  ++P +A GCGYDQ   S+   P   G+LGLGRG + ++
Sbjct: 133 QGSSMGALVTDQFPLKLVNGSFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLL 192

Query: 219 SQLHNQGIVRNVVGHCFNSKGGGYLFFGDGIYDPYRLVWTP-MSRDYPKHYSPGFGELIF 278
           +QL + G+ RNVVGHC +SKGGG+LFFGD +     + WTP +S+D   HY+ G  +L+F
Sbjct: 193 TQLVSAGLTRNVVGHCLSSKGGGFLFFGDNLVPSIGVAWTPLLSQD--NHYTTGPADLLF 252

Query: 279 NGRSTGLRNLFVVFDSGSSYTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGR 338
           NG+ TGL+ L ++FD+GSSYTYFN++AYQ + +L+  +L   PL+ A +D TLP+CW+G 
Sbjct: 253 NGKPTGLKGLKLIFDTGSSYTYFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGA 312

Query: 339 KPIKSLRDVRKYFKPLALSFSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLEN 398
           KP KS+ +V+ +FK + ++F++G R+  ++  P E Y+I+S  GNVCLG+LNG++VGL+N
Sbjct: 313 KPFKSVLEVKNFFKTITINFTNGRRNTQLYLAP-ELYLIVSKTGNVCLGLLNGSEVGLQN 372

Query: 399 SNIIGDISMQDKMVVYNNEKQAIGWATANCDRVPKS 425
           SN+IGDISMQ  M++Y+NEKQ +GW +++C+++PK+
Sbjct: 373 SNVIGDISMQGLMMIYDNEKQQLGWVSSDCNKLPKT 405

BLAST of CsGy2G016097 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 411.8 bits (1057), Expect = 6.9e-115
Identity = 193/378 (51.06%), Postives = 263/378 (69.58%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++V P+ GNVYP G+Y V L +G PPK + LD DTGSDLTW+QCDAPC  CT+     
Sbjct: 50  SSTVVFPVSGNVYPLGYYYVLLNIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQ 109

Query: 111 YQPSNDLVPCKDPLCMSLHSSMDHRCENP-DQCDYEVEYADGGSSLGVLVRDVFPLNLTN 170
           Y+P+++ +PC   LC  L    D  C +P DQCDYE+ Y+D  SS+G LV D  PL L N
Sbjct: 110 YKPNHNTLPCSHILCSGLDLPQDRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLAN 169

Query: 171 GDPIRPRLALGCGYD-QDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNS 230
           G  +  RL  GCGYD Q+PG     P  GILGLGRG V + +QL + GI +NV+ HC + 
Sbjct: 170 GSIMNLRLTFGCGYDQQNPGPHPPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSH 229

Query: 231 KGGGYLFFGDGIYDPYRLVWTPMSRDYP-KHYSPGFGELIFNGRSTGLRNLFVVFDSGSS 290
            G G+L  GD +     + WT ++ + P K+Y  G  EL+FN ++TG++ + VVFDSGSS
Sbjct: 230 TGKGFLSIGDELVPSSGVTWTSLATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSS 289

Query: 291 YTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALS 350
           YTYFNA+AYQ +  L+ ++L GKPL +  DD +LP+CW+G+KP+KSL +V+KYFK + L 
Sbjct: 290 YTYFNAEAYQAILDLIRKDLNGKPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLR 349

Query: 351 FSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNE 410
           F +  ++  +F++P E Y+II+  G VCLGILNGT++GLE  NIIGDIS Q  MV+Y+NE
Sbjct: 350 FGN-QKNGQLFQVPPESYLIITEKGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNE 409

Query: 411 KQAIGWATANCDRVPKSQ 426
           KQ IGW +++CD++PKS+
Sbjct: 410 KQRIGWISSDCDKLPKSE 426

BLAST of CsGy2G016097 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 407.5 bits (1046), Expect = 1.3e-113
Identity = 191/375 (50.93%), Postives = 260/375 (69.33%), Query Frame = 0

Query: 51  SSSIVLPLQGNVYPNGFYNVTLYVGQPPKPYFLDPDTGSDLTWLQCDAPCQQCTETLHPL 110
           SS++V P+ GNVYP G+Y V L +G PPK + LD DTGSDLTW+QCDAPC  CT+     
Sbjct: 50  SSTVVFPVSGNVYPLGYYYVLLNIGNPPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQ 109

Query: 111 YQPSNDLVPCKDPLCMSLHSSMDHRCENP-DQCDYEVEYADGGSSLGVLVRDVFPLNLTN 170
           Y+P+++ +PC   LC  L    D  C +P DQCDYE+ Y+D  SS+G LV D  PL L N
Sbjct: 110 YKPNHNTLPCSHILCSGLDLPQDRPCADPEDQCDYEIGYSDHASSIGALVTDEVPLKLAN 169

Query: 171 GDPIRPRLALGCGYD-QDPGSSSYHPMDGILGLGRGAVSIVSQLHNQGIVRNVVGHCFNS 230
           G  +  RL  GCGYD Q+PG     P  GILGLGRG V + +QL + GI +NV+ HC + 
Sbjct: 170 GSIMNLRLTFGCGYDQQNPGPHPPPPTAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSH 229

Query: 231 KGGGYLFFGDGIYDPYRLVWTPMSRDYP-KHYSPGFGELIFNGRSTGLRNLFVVFDSGSS 290
            G G+L  GD +     + WT ++ + P K+Y  G  EL+FN ++TG++ + VVFDSGSS
Sbjct: 230 TGKGFLSIGDELVPSSGVTWTSLATNSPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSS 289

Query: 291 YTYFNAQAYQVLTSLLNRELAGKPLREAMDDDTLPLCWRGRKPIKSLRDVRKYFKPLALS 350
           YTYFNA+AYQ +  L+ ++L GKPL +  DD +LP+CW+G+KP+KSL +V+KYFK + L 
Sbjct: 290 YTYFNAEAYQAILDLIRKDLNGKPLTDTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLR 349

Query: 351 FSSGGRSKAVFEIPTEGYMIISSMGNVCLGILNGTDVGLENSNIIGDISMQDKMVVYNNE 410
           F +  ++  +F++P E Y+II+  G VCLGILNGT++GLE  NIIGDIS Q  MV+Y+NE
Sbjct: 350 FGN-QKNGQLFQVPPESYLIITEKGRVCLGILNGTEIGLEGYNIIGDISFQGIMVIYDNE 409

Query: 411 KQAIGWATANCDRVP 423
           KQ IGW +++CD++P
Sbjct: 410 KQRIGWISSDCDKLP 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0IU524.3e-9347.04Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S... [more]
A2ZC674.1e-8844.82Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=... [more]
Q9M9A85.4e-8042.82Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Q9S9K43.8e-3328.31Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q8VYV96.3e-2830.40Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_004147327.20.0100.00aspartic proteinase Asp1 isoform X1 [Cucumis sativus] >KAE8651999.1 hypothetical... [more]
XP_008460823.10.098.83PREDICTED: aspartic proteinase Asp1 isoform X2 [Cucumis melo][more]
TYK02025.10.098.83aspartic proteinase Asp1 isoform X2 [Cucumis melo var. makuwa][more]
XP_008460822.10.097.92PREDICTED: aspartic proteinase Asp1 isoform X1 [Cucumis melo][more]
XP_038900559.10.096.26aspartic proteinase Asp1 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A5D3BS690.098.83Aspartic proteinase Asp1 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3CDB20.098.83aspartic proteinase Asp1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4... [more]
A0A1S3CDB40.097.92aspartic proteinase Asp1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499584 PE=4... [more]
A0A6J1I5904.20e-29890.00aspartic proteinase Asp1-like OS=Cucurbita maxima OX=3661 GN=LOC111469733 PE=4 S... [more]
A0A6J1DZ155.74e-29889.95aspartic proteinase Asp1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110243... [more]
Match NameE-valueIdentityDescription
AT4G33490.21.1e-16569.44Eukaryotic aspartyl protease family protein [more]
AT4G33490.16.4e-15370.14Eukaryotic aspartyl protease family protein [more]
AT1G44130.19.3e-12049.49Eukaryotic aspartyl protease family protein [more]
AT1G77480.26.9e-11551.06Eukaryotic aspartyl protease family protein [more]
AT1G77480.11.3e-11350.93Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 68..238
e-value: 3.7E-48
score: 164.1
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 280..414
e-value: 1.2E-15
score: 57.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 47..237
e-value: 2.6E-45
score: 156.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 242..422
e-value: 3.0E-28
score: 100.4
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 60..421
NoneNo IPR availablePANTHERPTHR13683:SF800EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 8..423
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..18
score: 6.0
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 8..423
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 68..414
score: 34.367401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G016097.1CsGy2G016097.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity