CsGy2G005940 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G005940
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description(R)-mandelonitrile lyase 1-like
LocationGy14Chr2: 4224921 .. 4228215 (-)
RNA-Seq ExpressionCsGy2G005940
SyntenyCsGy2G005940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGACACACTGCTGTATAAAAACAAAAATTAACCAAACCACAGCTTGCAATTAAATTAAGTGTCCAGCTTTGTAATCCTAAATATATATCTATATAAACCAACAGTTTGCAAATTAGAAAGGAACAAAGAAGTACCCCCACATATACATCTACATCTTTGAATAAGCATGGAGTATTCAATAGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGGCAAAACCCTGATCTCTCTATATCTTTCTTTTTCTAAACTTGTATCGTGTTTAGTTTATTGATTCTAACTGTTGTGTATTTTGTTACCTTTCCTTTTCTAGATATTAGCTACATGAAATTTGTACACAATGCCAATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCTTTTGTGTTGAATGAAGAAACTCTATCCTACACTTTTATTCTAGACGATGATGGCCAAAATCCCATCCAACGCTTTGTCTCTGAGGATGGTGTGGAGAACATACGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAACGCCGGGTTCTACTCGAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTGCATGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAGAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTAGTGGCTAACAAGTTAAATTTGGTCCTTTTTTTTTTTGGTTAATGAATTTTTAGAAGATGGTTTCTAACTTTTAAAAAGATAAGGGTTTTATATATATATATTGATATTAGAGAAAAAAATAATGATATTTTGGTCCATTTTAAGGTGCACTTTCATCACCCAACTCACTCACTGATCATCATGAAAGTAAAATATAATTTTTTAAAAAATTAATCTTTTCAGTACTCAAATATAATTTTGAAGAATAATGTGGGTTGGATTCTTAAGTTTTTAAAACACACCTTATCCTGTTATTATATAAAACTAAAATAGAAAATAGTCCATGCACCTTTTGAACATTTTTTTTTTAATTTCAAATTTAAAAACATATATTTTTAAAACTCAAGGAAAAATAGACTCGTTTTTTTTTTTTTTTTTAAGAATTAAAAGATCAAAACTCACTGGTTCATAGACTAATACAGTACTAGTTTGCAGAATAAAAAATAAATATTTTTTAAGAAAATGGTGATGATCCAGTCTGTGTGTCTCCAAAACATAATCACATCAATGACATGTTAATAAATGCATGACAACGATATACCAATGGTTAAAACTTAAAAGCAAAACGAAATTAAACTTATCATCTCTACCTAAATAAATAATAAATACTTATGTTTACTACAAAATCTACAACCTACAATTTTTGAAACGTTGACAATTTTATGGGAAAACAAATGTCAAGTAATGTACTTAGAAACGTAAAATAGATAACATAATAAGCAAAAATTTCACAGAAGTTTTCGATCTTATTTTTTTCACGTTTTTCTACTTCCAACTGACAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGGCATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATCTCGTTCACCACCAACCACATGTGGGTCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTTGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTATTGTAGGTAAATTTTCTAATGTTAGTTCTAAAGGATGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTTAGTGGAGTGAGGAAAGTGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAAAGGATTTCAATTTATGGAACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTATTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTGATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCGGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGGTGAGTTTATTTATTTATTTATCTTTTTACCTGGTGGATCTCTCAAAATATATATATGTCTGTATGTGTGTGGTTTGATTAAGTTTGGTTTGGTTTTTGGTTTAATATATAATATAAACAGTTTGTGTTTTGAACCTTGATACCTGTTTATAAGAAGCATTTTTGTAGTAAAAAAAAATGCTACTTCTTGTTATTATTTGAAATAAATGAAGGGCTTGGACCTTTGCACTATTAGTTGAACTTAGTTTTTTCTTTCTCTTTTGCATTATCTTTTATTTCCATAATTAAACTTACAAGTTCTAACTTGTTTTGCAGATATGTTGGCCTTAAAGTACTGCAACAAAGATCAAGCTAAAAGTGTAAATCAAGATAAATGATGAGAGATTTAAATCAGGGACTTTTAATTTAATTTCCTTACTAACATATTGTGGATGCATCTTACTTTCACTACTATATGTCTTTGTTTAGATGATATAAGGTTCAATTTACTTTTACCCATATGCTTAAATGATTGGGTCCAATCATTTATCATTGCTACTGGAGAATATGGTCCTGAATCCTTGCCTGTTTCAGTCAATAACCATTTAAGATGTTTAATAAATATTCTTTTTCGTTTACATTA

mRNA sequence

TGGACACACTGCTGTATAAAAACAAAAATTAACCAAACCACAGCTTGCAATTAAATTAAGTGTCCAGCTTTGTAATCCTAAATATATATCTATATAAACCAACAGTTTGCAAATTAGAAAGGAACAAAGAAGTACCCCCACATATACATCTACATCTTTGAATAAGCATGGAGTATTCAATAGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGATATTAGCTACATGAAATTTGTACACAATGCCAATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCTTTTGTGTTGAATGAAGAAACTCTATCCTACACTTTTATTCTAGACGATGATGGCCAAAATCCCATCCAACGCTTTGTCTCTGAGGATGGTGTGGAGAACATACGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAACGCCGGGTTCTACTCGAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTGCATGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAGAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGGCATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATCTCGTTCACCACCAACCACATGTGGGTCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTTGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTATTGTAGGTAAATTTTCTAATGTTAGTTCTAAAGGATGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTTAGTGGAGTGAGGAAAGTGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAAAGGATTTCAATTTATGGAACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTATTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTGATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCGGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGATATGTTGGCCTTAAAGTACTGCAACAAAGATCAAGCTAAAAGTGTAAATCAAGATAAATGATGAGAGATTTAAATCAGGGACTTTTAATTTAATTTCCTTACTAACATATTGTGGATGCATCTTACTTTCACTACTATATGTCTTTGTTTAGATGATATAAGGTTCAATTTACTTTTACCCATATGCTTAAATGATTGGGTCCAATCATTTATCATTGCTACTGGAGAATATGGTCCTGAATCCTTGCCTGTTTCAGTCAATAACCATTTAAGATGTTTAATAAATATTCTTTTTCGTTTACATTA

Coding sequence (CDS)

ATGGAGTATTCAATAGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGATATTAGCTACATGAAATTTGTACACAATGCCAATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCTTTTGTGTTGAATGAAGAAACTCTATCCTACACTTTTATTCTAGACGATGATGGCCAAAATCCCATCCAACGCTTTGTCTCTGAGGATGGTGTGGAGAACATACGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAACGCCGGGTTCTACTCGAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTGCATGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAGAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGGCATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATCTCGTTCACCACCAACCACATGTGGGTCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTTGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTATTGTAGGTAAATTTTCTAATGTTAGTTCTAAAGGATGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTTAGTGGAGTGAGGAAAGTGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAAAGGATTTCAATTTATGGAACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTATTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTGATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCGGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGATATGTTGGCCTTAAAGTACTGCAACAAAGATCAAGCTAA

Protein sequence

MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS*
Homology
BLAST of CsGy2G005940 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.9e-149
Identity = 260/549 (47.36%), Postives = 368/549 (67.03%), Query Frame = 0

Query: 4   SIATFLPLILISIEFLSSHAIPN---QDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 63
           ++   L + ++ +++    ++ N    D SY+ FV++A D  ++  YDYI++GGGT GCP
Sbjct: 8   AVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCP 67

Query: 64  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 123
           LA TLS+ +SVL+LERGS P  YP +L  +   Y    +DDG+ P++RFVSEDG++N+RG
Sbjct: 68  LAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 124 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 183
           RVLGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V  P+   WQ     
Sbjct: 128 RVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGT 187

Query: 184 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 243
             LE G++P+NGF + HL GT++ GS FDN G RH + ELLNK +P NL+V +QA V++I
Sbjct: 188 AYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKI 247

Query: 244 IF----KGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSS 303
           IF     G++A+GV+Y+DS G  H A +  +GE+ LSAG IGSPQLLLLSG+GP+SYL+S
Sbjct: 248 IFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTS 307

Query: 304 LKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLAS------ 363
           L + +V   P+VGQY+ DNPR   +I+ P  + AST  V+G +  + +  S++S      
Sbjct: 308 LNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLG-ITSDFYQCSISSLPFDTP 367

Query: 364 PLPFFPLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYY 423
           P  FFP  SY L  P  T        IV K     S G + L NSS+D +  P V+FNYY
Sbjct: 368 PFSFFPTTSYPL--PNQT-----FAHIVNKVPGPLSHGTVTL-NSSSDVRVGPNVKFNYY 427

Query: 424 SQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYC 483
           S   DLS CVSG++K+ ++L T  +E  K +DL G  GF  + +P+PEN  +D++ E +C
Sbjct: 428 SNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFC 487

Query: 484 KNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVG 540
           + +VA+YWHYHGGCLVGKV+DD ++V GI  LRVVDGSTF  +P ++P    +MLGRY+G
Sbjct: 488 RESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMG 547

BLAST of CsGy2G005940 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 9.3e-146
Identity = 258/543 (47.51%), Postives = 363/543 (66.85%), Query Frame = 0

Query: 4   SIATFLPLILISIEFLSSHAI---PNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 63
           +I   L L ++ +++   H++    N D SY++F ++A D  ++  YDY+++GGGT+GCP
Sbjct: 8   AILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 64  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 123
           LA TLS K+ VL+LERGS P  YP VL  +   Y    +DDG+ P++RFVSEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 124 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 183
           RVLGG SM+NAG Y+R +   +  +GVDWDM+LV K YEWVE+T+V +PN   WQ+    
Sbjct: 128 RVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGT 187

Query: 184 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 243
             LE GV P++GF L H  GT+I GS FDNKG RH A ELLNK N  NL+V + A+V++I
Sbjct: 188 AFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKI 247

Query: 244 IFK---GLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSL 303
           IF    GL+A GV+Y DS G  H A +  KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL
Sbjct: 248 IFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 307

Query: 304 KLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPL 363
            + +V   P+VGQ++ DNPR   +I+ P  +  +   V+G    N   Q   S LP F  
Sbjct: 308 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP-FTT 367

Query: 364 PSYGLLPPQSTSI-TSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDL 423
           P +   P  S  +  S+      K +   S G L L  SS++ + +P V+FNYYS   DL
Sbjct: 368 PPFSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTL-KSSSNVRVSPNVKFNYYSNPTDL 427

Query: 424 SRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVAT 483
           S CVSG++K+ +LL T  ++  K +DL G +GF  + +P+P++  +D++ E +C+ +VA+
Sbjct: 428 SHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVAS 487

Query: 484 YWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQ 540
           YWHYHGGCLVGKV+D +++V GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+LQ+
Sbjct: 488 YWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 546

BLAST of CsGy2G005940 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-145
Identity = 256/538 (47.58%), Postives = 364/538 (67.66%), Query Frame = 0

Query: 9   LPLILISIEFLSSHAIPN---QDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTL 68
           L L+++ +++   H++ N    D SY+KFV+NA D  ++  YDYIVIGGGT+GCPLA TL
Sbjct: 13  LHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATL 72

Query: 69  SSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGG 128
           S K+ VLLLERG+    YP  L  +  +Y     DDG+ P++RFVSEDG++N+R R+LGG
Sbjct: 73  SEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGG 132

Query: 129 GSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEG 188
            +++NAG Y+R +  F+   G++WD++LV K YEWVE+ +V +PN  +WQ+      LE 
Sbjct: 133 TTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEA 192

Query: 189 GVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF--- 248
           G++PDNGF L H  GT++ GS FDN G RH A ELLNK +P NL V +QA+V++I+F   
Sbjct: 193 GILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSN 252

Query: 249 -KGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHL 308
              LSA+GV+Y+DS G  H A +   GE+ +SAG IG+PQLLLLSG+GP+SYLSSL + +
Sbjct: 253 TSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITV 312

Query: 309 VHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYG 368
           V   P+VGQ++ +NPR   +   P  + AS   V+G   ++ + Q   S LP F  P + 
Sbjct: 313 VQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGI--RSDYYQVSLSSLP-FSTPPFS 372

Query: 369 LLPPQSTSI-TSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 428
           L P  S  +  S+   IV +     S G + L NSS+D +  P ++FNYYS   DL+ CV
Sbjct: 373 LFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTL-NSSSDVRIAPNIKFNYYSNSTDLANCV 432

Query: 429 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHY 488
           SG++K+ DLL+T+ +E  K +D+ G  GF ++ +P+PEN  +D+S E +C + VA+YWHY
Sbjct: 433 SGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHY 492

Query: 489 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRS 539
           HGG LVGKV+DD+++V+GIK LRVVD STF   P ++P    +MLGRYVGL++LQ+RS
Sbjct: 493 HGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546

BLAST of CsGy2G005940 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.9e-145
Identity = 255/543 (46.96%), Postives = 365/543 (67.22%), Query Frame = 0

Query: 4   SIATFLPLILISIEFLSSHAI---PNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 63
           +I   L + ++ +++   H++    + D SY+ F ++A D  ++  YDY+++GGGT+GCP
Sbjct: 8   AILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 64  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 123
           LA TLS K+ VL+LERGS P  YP VL  +   Y    +DDG+ P++RFVSEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 124 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 183
           RVLGG S++NAG Y+R +   +  +GVDWDM+LV + YEWVE+T+V +PN  +WQ+  + 
Sbjct: 128 RVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKT 187

Query: 184 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 243
             LE GV P++GF L H  GT+I GS FDNKG RH A ELLNK N  NL+V + A+V++I
Sbjct: 188 AFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKI 247

Query: 244 IFK---GLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSL 303
           IF    GL+A GV+Y DS G  H A +  KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL
Sbjct: 248 IFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 307

Query: 304 KLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPL 363
            + +V   P+VGQ++ DNPR   +I+ P  +  +   V+G    N   Q   S LP F  
Sbjct: 308 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP-FTT 367

Query: 364 PSYGLLPPQSTSI-TSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDL 423
           P +G  P  S  +  S+      K +   S G L L  SS++ + +P V+FNYYS   DL
Sbjct: 368 PPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTL-KSSSNVRVSPNVKFNYYSNLTDL 427

Query: 424 SRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVAT 483
           S CVSG++K+ +LL T  ++  K +DL G +GF  + +P+P++  +D++ E +C+ +VA+
Sbjct: 428 SHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVAS 487

Query: 484 YWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQ 540
           YWHYHGGCLVGKV+D +++V GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+LQ+
Sbjct: 488 YWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 546

BLAST of CsGy2G005940 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.3e-144
Identity = 255/542 (47.05%), Postives = 362/542 (66.79%), Query Frame = 0

Query: 9   LPLILISIEFLSSHAIPN---QDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTL 68
           L L ++ +++    ++ N    D SY++FV++AND  ++  YDYI++GGGT GCPLA TL
Sbjct: 14  LHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATL 73

Query: 69  SSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGG 128
           S+ +SVL+LERG+ P  YP +L  +   Y    +DDGQ P++RFVS DG++N+RGRVLGG
Sbjct: 74  SANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGG 133

Query: 129 GSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEG 188
            SM+NAG Y R +  FF   G++WDM+LV K Y+WVE+T+V +P+   WQ       LE 
Sbjct: 134 TSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEV 193

Query: 189 GVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF--- 248
           G++PDNGF L HL GT++ GS FDN G RH + ELLNK +P NL+V + A V++IIF   
Sbjct: 194 GILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKIIFSSD 253

Query: 249 -KGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHL 308
             G++A+GV+Y+DS G  H A +   GE+ LSAG IGSPQLLLLSG+G +SYL+SL + +
Sbjct: 254 SSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTSLNISV 313

Query: 309 VHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLAS------PLPFF 368
           V   P+VGQY+ DNPR   +I+ P  + AST  V+G +  + +  S++S      P  FF
Sbjct: 314 VASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLG-ITSDFYQCSISSLPFSTAPFGFF 373

Query: 369 PLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD 428
           P P+Y L  P +T        IV K     S G + L  S++D +  P V FNYYS   D
Sbjct: 374 PNPTYPL--PNTT-----FAHIVNKVPGPLSHGTV-LLQSTSDVRVAPNVTFNYYSNTTD 433

Query: 429 LSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVA 488
           L+ CVSG++K+ + L +  ++  K +DL G +GF  + +P+PEN  +D++ E +C+  VA
Sbjct: 434 LAHCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVA 493

Query: 489 TYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQ 538
           +YWHYHGGCLVG+V+DD+++V GI  LRVVDGSTF  +P ++P    +MLGRY+G K+LQ
Sbjct: 494 SYWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQ 546

BLAST of CsGy2G005940 vs. NCBI nr
Match: XP_011648923.1 ((R)-mandelonitrile lyase 3 [Cucumis sativus] >KGN61116.1 hypothetical protein Csa_021230 [Cucumis sativus])

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 537/539 (99.63%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP
Sbjct: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG
Sbjct: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN
Sbjct: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
           TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI
Sbjct: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240

Query: 241 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300
           IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH
Sbjct: 241 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300

Query: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360
           LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY
Sbjct: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360

Query: 361 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 420
           GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD+SRCV
Sbjct: 361 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCV 420

Query: 421 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHY 480
           SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSS+EEYCKNTVATYWHY
Sbjct: 421 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480

Query: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539
           HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS
Sbjct: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539

BLAST of CsGy2G005940 vs. NCBI nr
Match: XP_008451913.1 (PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo])

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 510/540 (94.44%), Postives = 527/540 (97.59%), Query Frame = 0

Query: 1   MEYSIATFLPL-ILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC 60
           ME+S+ATFL L ILISIEF+SSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC
Sbjct: 1   MEHSLATFLLLLILISIEFISSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC 60

Query: 61  PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIR 120
           PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSY FIL+DDGQNPIQRFVSEDGVENIR
Sbjct: 61  PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYVFILEDDGQNPIQRFVSEDGVENIR 120

Query: 121 GRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFR 180
           GRVLGGGSMVNAGFYSRGH+EFFETAGVDW+MELVEKAYEWVEETLVSRPNL+AWQ AFR
Sbjct: 121 GRVLGGGSMVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFR 180

Query: 181 NTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR 240
           NTLLEGGVVPDNGFDLRHL+GTK GGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR
Sbjct: 181 NTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR 240

Query: 241 IIFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKL 300
           IIF GLSAVGVLYSDSKGKLHTALIHKKGEI LSAGAIGSPQLLLLSGIGPKSYLSSLKL
Sbjct: 241 IIFNGLSAVGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKL 300

Query: 301 HLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPS 360
           H+VHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS+AQVVGTLEKNIHLQSLASPLPF+PLPS
Sbjct: 301 HVVHHQPHVGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPS 360

Query: 361 YGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRC 420
           YGLLPPQSTSIT SLV+ VGKFSNVSSKGWLHLNNSSTD+KE+PIVRFNYYSQH DLSRC
Sbjct: 361 YGLLPPQSTSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRC 420

Query: 421 VSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWH 480
           V GVRKVEDLLKTQTMER KTQDLEGNKGFQFM LPMPENLWNDSS+EEYCKNTVATYWH
Sbjct: 421 VRGVRKVEDLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWH 480

Query: 481 YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539
           YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS
Sbjct: 481 YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 540

BLAST of CsGy2G005940 vs. NCBI nr
Match: KAA0067297.1 ((R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa] >TYK10908.1 (R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 471/496 (94.96%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 32  MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 91
           MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET
Sbjct: 1   MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 60

Query: 92  LSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDM 151
           LSY FIL+DDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFETAGVDW+M
Sbjct: 61  LSYVFILEDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWEM 120

Query: 152 ELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNK 211
           ELVEKAYEWVEETLVSRPNL+AWQ AFRNTLLEGGVVPDNGFDLRHL+GTK GGSIFDNK
Sbjct: 121 ELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNK 180

Query: 212 GNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIF 271
           GNRHGAVELLNKANPKNLKVVIQATVQRIIF GLSAVGVLYSDSKGKLHTALIHKKGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSAVGVLYSDSKGKLHTALIHKKGEIL 240

Query: 272 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLAS 331
           LSAGAIGSPQLLLLSGIGPKSYLSSLKLH+VHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQFMTDNPRFSRSIVFPFQLLAS 300

Query: 332 TAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLH 391
           +AQVVGTLEKNIHLQSLASPLPF+PLPSYGLLPPQSTSIT SLV+ VGKFSNVSSKGWLH
Sbjct: 301 SAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQSTSITPSLVVFVGKFSNVSSKGWLH 360

Query: 392 LNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQF 451
           LNNSSTD+KE+PIVRFNYYSQH DLSRCV GVRKVEDLLKTQTMER KTQDLEGNKGFQF
Sbjct: 361 LNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVEDLLKTQTMERFKTQDLEGNKGFQF 420

Query: 452 MELPMPENLWNDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 511
           M LPMPENLWNDSS+EEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS
Sbjct: 421 MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 480

Query: 512 DSPGTNPMATLMMLGR 527
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of CsGy2G005940 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 839 bits (2168), Expect = 6.23e-303
Identity = 421/542 (77.68%), Postives = 469/542 (86.53%), Query Frame = 0

Query: 1   MEYSIATFLPLILISIEFL---SSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTT 60
           M+ S+AT L +ILISI  L   SSH IP+QD SY+KFVH AN+ P   +YDYI+IGGGT 
Sbjct: 1   MKRSMATILLIILISIFQLGVFSSHTIPDQDTSYLKFVHEANELPETGEYDYIIIGGGTA 60

Query: 61  GCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVEN 120
           GCPLA TLSSK+SVLLLERGSDPN YP VLNE+ L   F+ +DDGQNP Q F+SEDGVEN
Sbjct: 61  GCPLAATLSSKYSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVEN 120

Query: 121 IRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAA 180
           IRGRVLGGGSM+NAGFYSRGH+EFFETAGVDWDMELVEKAY+WVE+T+VSRP+L+AWQ+A
Sbjct: 121 IRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEQTVVSRPSLSAWQSA 180

Query: 181 FRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATV 240
           FR  LLE GV PDNGFDL+HLVGTK GGSIFDNKGNRHGAVELLNKA P+NLKV  QA V
Sbjct: 181 FRGALLEAGVGPDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVGTQAIV 240

Query: 241 QRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSL 300
           +RIIF GLSA GV YSD KGKLHTA I +KGEI LSAGAIGSPQLLLLSG+GPK +LSSL
Sbjct: 241 ERIIFTGLSASGVSYSDPKGKLHTAFIREKGEIILSAGAIGSPQLLLLSGVGPKYHLSSL 300

Query: 301 KLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPL 360
           KL +V HQPHVG++M+DNPRFS +I+ PFQL+AS+AQVVGTL  NIHLQS ASPLPFF  
Sbjct: 301 KLPVVLHQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLANNIHLQSFASPLPFFAP 360

Query: 361 PSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLS 420
           PS+ LLPPQSTSI  SL I VGKFS+V S+G L LN SS D KE+PIVRFNYYS H DL+
Sbjct: 361 PSFSLLPPQSTSIIPSLAIFVGKFSDVLSEGSLRLN-SSIDVKESPIVRFNYYSNHDDLA 420

Query: 421 RCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATY 480
           RCV GVRKV DLLKTQTME+IK QDLEGNKGFQF+ LP+PENLWNDS++EEYC+ TV TY
Sbjct: 421 RCVRGVRKVGDLLKTQTMEKIKNQDLEGNKGFQFLGLPLPENLWNDSAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQR 539
           WHYHGGCLVGKVVD N+KVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGLKVLQQR
Sbjct: 481 WHYHGGCLVGKVVDHNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQR 540

BLAST of CsGy2G005940 vs. NCBI nr
Match: XP_008452065.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 830 bits (2145), Expect = 2.05e-299
Identity = 409/523 (78.20%), Postives = 454/523 (86.81%), Query Frame = 0

Query: 16  IEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLE 75
           ++ LSS AIPNQD+SYMKFVHNA D P +E+YDYI+IGGGT GCPLATTLSSKFS+L+LE
Sbjct: 20  LQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSILVLE 79

Query: 76  RGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYS 135
           RGSDPN YP VLN + ++  F   DDG+NP Q FVSEDGVENIRGRVLGGGSMVNAGFYS
Sbjct: 80  RGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVNAGFYS 139

Query: 136 RGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLT-AWQAAFRNTLLEGGVVPDNGFD 195
           RGH+EFFE AGVDWDMELVEKAYEWVEET+VS+P L  AWQAAFR+ LLEGGVVPDNGFD
Sbjct: 140 RGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVPDNGFD 199

Query: 196 LRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSD 255
           LRHLVGTKIGGSIFDNKGNRHGAVELLNKANP N+KV IQATVQ+I+F GLSA GV YSD
Sbjct: 200 LRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSATGVSYSD 259

Query: 256 SKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTD 315
           SKGKLHTA IHK GEI +SAGAIGSPQLLLLSGIGPKS+LSSLKL +V HQPHVGQ+MTD
Sbjct: 260 SKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQFMTD 319

Query: 316 NPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSL 375
           NPRF  +I+ PFQLL S  +VVG L++NI++QS A P  F   P++ LLPPQSTSI  +L
Sbjct: 320 NPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQSTSINPTL 379

Query: 376 VIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKVEDLLKTQT 435
              VGKFS+V+SKGWLHLN SS D K++PIVRFNYYS H DL RCV GVRKV DLLKTQT
Sbjct: 380 ASFVGKFSDVNSKGWLHLN-SSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVGDLLKTQT 439

Query: 436 MERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNY 495
           +E+IKTQDLEG K F F+ LP+ ENLWNDSS++EYCK TV TYWHYHGGCLVGKVVD NY
Sbjct: 440 IEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVGKVVDGNY 499

Query: 496 KVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQR 537
           KVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGLKVL+QR
Sbjct: 500 KVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLKQR 541

BLAST of CsGy2G005940 vs. ExPASy TrEMBL
Match: A0A0A0LMB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055040 PE=3 SV=1)

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 537/539 (99.63%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP
Sbjct: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG
Sbjct: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN
Sbjct: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
           TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI
Sbjct: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240

Query: 241 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300
           IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH
Sbjct: 241 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300

Query: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360
           LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY
Sbjct: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360

Query: 361 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 420
           GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD+SRCV
Sbjct: 361 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCV 420

Query: 421 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHY 480
           SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSS+EEYCKNTVATYWHY
Sbjct: 421 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480

Query: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539
           HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS
Sbjct: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539

BLAST of CsGy2G005940 vs. ExPASy TrEMBL
Match: A0A1S3BTE4 ((R)-mandelonitrile lyase 3-like OS=Cucumis melo OX=3656 GN=LOC103493064 PE=3 SV=1)

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 510/540 (94.44%), Postives = 527/540 (97.59%), Query Frame = 0

Query: 1   MEYSIATFLPL-ILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC 60
           ME+S+ATFL L ILISIEF+SSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC
Sbjct: 1   MEHSLATFLLLLILISIEFISSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC 60

Query: 61  PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIR 120
           PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSY FIL+DDGQNPIQRFVSEDGVENIR
Sbjct: 61  PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYVFILEDDGQNPIQRFVSEDGVENIR 120

Query: 121 GRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFR 180
           GRVLGGGSMVNAGFYSRGH+EFFETAGVDW+MELVEKAYEWVEETLVSRPNL+AWQ AFR
Sbjct: 121 GRVLGGGSMVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFR 180

Query: 181 NTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR 240
           NTLLEGGVVPDNGFDLRHL+GTK GGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR
Sbjct: 181 NTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR 240

Query: 241 IIFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKL 300
           IIF GLSAVGVLYSDSKGKLHTALIHKKGEI LSAGAIGSPQLLLLSGIGPKSYLSSLKL
Sbjct: 241 IIFNGLSAVGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKL 300

Query: 301 HLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPS 360
           H+VHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS+AQVVGTLEKNIHLQSLASPLPF+PLPS
Sbjct: 301 HVVHHQPHVGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPS 360

Query: 361 YGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRC 420
           YGLLPPQSTSIT SLV+ VGKFSNVSSKGWLHLNNSSTD+KE+PIVRFNYYSQH DLSRC
Sbjct: 361 YGLLPPQSTSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRC 420

Query: 421 VSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWH 480
           V GVRKVEDLLKTQTMER KTQDLEGNKGFQFM LPMPENLWNDSS+EEYCKNTVATYWH
Sbjct: 421 VRGVRKVEDLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWH 480

Query: 481 YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539
           YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS
Sbjct: 481 YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 540

BLAST of CsGy2G005940 vs. ExPASy TrEMBL
Match: A0A5D3CI61 ((R)-mandelonitrile lyase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00870 PE=3 SV=1)

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 471/496 (94.96%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 32  MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 91
           MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET
Sbjct: 1   MKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEET 60

Query: 92  LSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDM 151
           LSY FIL+DDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGH+EFFETAGVDW+M
Sbjct: 61  LSYVFILEDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHREFFETAGVDWEM 120

Query: 152 ELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNK 211
           ELVEKAYEWVEETLVSRPNL+AWQ AFRNTLLEGGVVPDNGFDLRHL+GTK GGSIFDNK
Sbjct: 121 ELVEKAYEWVEETLVSRPNLSAWQVAFRNTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNK 180

Query: 212 GNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTALIHKKGEIF 271
           GNRHGAVELLNKANPKNLKVVIQATVQRIIF GLSAVGVLYSDSKGKLHTALIHKKGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVVIQATVQRIIFNGLSAVGVLYSDSKGKLHTALIHKKGEIL 240

Query: 272 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLAS 331
           LSAGAIGSPQLLLLSGIGPKSYLSSLKLH+VHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSYLSSLKLHVVHHQPHVGQFMTDNPRFSRSIVFPFQLLAS 300

Query: 332 TAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLH 391
           +AQVVGTLEKNIHLQSLASPLPF+PLPSYGLLPPQSTSIT SLV+ VGKFSNVSSKGWLH
Sbjct: 301 SAQVVGTLEKNIHLQSLASPLPFYPLPSYGLLPPQSTSITPSLVVFVGKFSNVSSKGWLH 360

Query: 392 LNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQF 451
           LNNSSTD+KE+PIVRFNYYSQH DLSRCV GVRKVEDLLKTQTMER KTQDLEGNKGFQF
Sbjct: 361 LNNSSTDSKESPIVRFNYYSQHDDLSRCVRGVRKVEDLLKTQTMERFKTQDLEGNKGFQF 420

Query: 452 MELPMPENLWNDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 511
           M LPMPENLWNDSS+EEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS
Sbjct: 421 MGLPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFS 480

Query: 512 DSPGTNPMATLMMLGR 527
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of CsGy2G005940 vs. ExPASy TrEMBL
Match: A0A1S3BTS5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=1)

HSP 1 Score: 830 bits (2145), Expect = 9.90e-300
Identity = 409/523 (78.20%), Postives = 454/523 (86.81%), Query Frame = 0

Query: 16  IEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLE 75
           ++ LSS AIPNQD+SYMKFVHNA D P +E+YDYI+IGGGT GCPLATTLSSKFS+L+LE
Sbjct: 20  LQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSILVLE 79

Query: 76  RGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYS 135
           RGSDPN YP VLN + ++  F   DDG+NP Q FVSEDGVENIRGRVLGGGSMVNAGFYS
Sbjct: 80  RGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSMVNAGFYS 139

Query: 136 RGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLT-AWQAAFRNTLLEGGVVPDNGFD 195
           RGH+EFFE AGVDWDMELVEKAYEWVEET+VS+P L  AWQAAFR+ LLEGGVVPDNGFD
Sbjct: 140 RGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGVVPDNGFD 199

Query: 196 LRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSD 255
           LRHLVGTKIGGSIFDNKGNRHGAVELLNKANP N+KV IQATVQ+I+F GLSA GV YSD
Sbjct: 200 LRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSATGVSYSD 259

Query: 256 SKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTD 315
           SKGKLHTA IHK GEI +SAGAIGSPQLLLLSGIGPKS+LSSLKL +V HQPHVGQ+MTD
Sbjct: 260 SKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQFMTD 319

Query: 316 NPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSL 375
           NPRF  +I+ PFQLL S  +VVG L++NI++QS A P  F   P++ LLPPQSTSI  +L
Sbjct: 320 NPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQSTSINPTL 379

Query: 376 VIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKVEDLLKTQT 435
              VGKFS+V+SKGWLHLN SS D K++PIVRFNYYS H DL RCV GVRKV DLLKTQT
Sbjct: 380 ASFVGKFSDVNSKGWLHLN-SSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVGDLLKTQT 439

Query: 436 MERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNY 495
           +E+IKTQDLEG K F F+ LP+ ENLWNDSS++EYCK TV TYWHYHGGCLVGKVVD NY
Sbjct: 440 IEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVGKVVDGNY 499

Query: 496 KVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQR 537
           KVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGLKVL+QR
Sbjct: 500 KVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLKQR 541

BLAST of CsGy2G005940 vs. ExPASy TrEMBL
Match: A0A6J1HRM5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3 SV=1)

HSP 1 Score: 823 bits (2126), Expect = 6.41e-297
Identity = 410/537 (76.35%), Postives = 461/537 (85.85%), Query Frame = 0

Query: 6   ATFLPLILISIEFL---SSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLA 65
           AT LPLILISI FL   SSH IPNQD+SYMK VH+A D P  E+YDYI+IGGGT GCPLA
Sbjct: 2   ATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLA 61

Query: 66  TTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRV 125
            TLSSKFSVLLLERGSDPN YP VLNE+ L   F+ +DDG+NP QRFVSE+GVE IRGRV
Sbjct: 62  ATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRV 121

Query: 126 LGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTL 185
           LGGGSMVNAGFYSRGH+EFFE+AGVDWDMELVEK YEWVEET+VSRP L+AWQ AFR  L
Sbjct: 122 LGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRAL 181

Query: 186 LEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF 245
           LEGGV PDNGFDLRHLVGTK GGSIFD  GNRHGAVELLN   P NLKV ++ATVQ+IIF
Sbjct: 182 LEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIF 241

Query: 246 KGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLV 305
             LSA+GV YSDS+GK HTA I KKGEI LSAGAIGSPQLLLLSG+GPKS+LSSLKL +V
Sbjct: 242 SDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVV 301

Query: 306 HHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGL 365
            HQPHVG++M+DNPRFS +++ PF L+AS+AQVVG L+ NIHLQ+ ASP PFF LP++GL
Sbjct: 302 LHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFGL 361

Query: 366 LPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSG 425
           LPPQST I  SL +++GKFS+V SKG LHLN SSTD K NPIVRFNYY+   DL++CV G
Sbjct: 362 LPPQSTLINPSLALLIGKFSDVLSKGSLHLN-SSTDVKNNPIVRFNYYTNPNDLAQCVRG 421

Query: 426 VRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVATYWHYHG 485
           +RKV D LKT+TME IKTQDLEGNKGFQF+  P+PENL ++SS++EYCK TV TYWHYHG
Sbjct: 422 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 481

Query: 486 GCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQQRSS 539
           GCLVGKVVD NY+VIGIKNLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+LQ+RSS
Sbjct: 482 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 537

BLAST of CsGy2G005940 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 497.7 bits (1280), Expect = 1.2e-140
Identity = 259/542 (47.79%), Postives = 355/542 (65.50%), Query Frame = 0

Query: 7   TFLPLILISIEFLSSHAIP--NQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATT 66
           T L ++L+      S+A P  N+   +M+F+ NA D   ++ YDYI++GGGT GCPLA T
Sbjct: 12  TALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAAT 71

Query: 67  LSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLG 126
           LS  F VLLLERG  P   P V++ +    T    ++  +P Q F+SE+GV N RGRVLG
Sbjct: 72  LSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLG 131

Query: 127 GGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLE 186
           G S +NAGFYSR  ++FFE +G+ WD+  V ++YEWVE  +V RP L  WQ A R+ LLE
Sbjct: 132 GSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLE 191

Query: 187 GGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF-- 246
            GV P NGF L H VGTKIGGS FD  G RH + +LL  A   N++V + ATV+R++   
Sbjct: 192 VGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLAS 251

Query: 247 ------KGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSS 306
                   +SA+GV+Y D  G+ H ALI  +GE+ LSAGA+GSPQLL LSGIGP+SYLS+
Sbjct: 252 SPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLST 311

Query: 307 LKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPF-F 366
             + +   QPHVG ++ DNPR   SI+ P  +  S  QVVG  E    L++ ++ +PF  
Sbjct: 312 WGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFAS 371

Query: 367 PLPSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD 426
           PL S  +  P S  +   +  I+ K     S G L L  +STD + NP+VRFNY+S   D
Sbjct: 372 PLHSVFIRAPAS-PLYVPVTTIMEKILGPVSIGLLRL--ASTDVRINPVVRFNYFSDPQD 431

Query: 427 LSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSIEEYCKNTVA 486
           L RCV+G RK+ ++L+++ M+    ++  GN+ F+F+  P+P +  ND  + ++C+ TV+
Sbjct: 432 LERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVS 491

Query: 487 TYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQ 538
           T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLGRY+GLK+L+
Sbjct: 492 TIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLR 550

BLAST of CsGy2G005940 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.6e-111
Identity = 234/560 (41.79%), Postives = 317/560 (56.61%), Query Frame = 0

Query: 18  FLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERG 77
           FL S    +       F+ +A   P    YDYI+IGGGT GCPLA TLS   SVLLLERG
Sbjct: 17  FLHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERG 76

Query: 78  SDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRG 137
             P   P +          + D    +P QRFVSEDGV N R RVLGGGS +NAGFY+R 
Sbjct: 77  DSPYNNPNITRLSAFG-AALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRA 136

Query: 138 HQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRH 197
             ++    G  WD  L  ++Y+WVE  +  +P +  WQ A R+ LLE G+VP+NGF   H
Sbjct: 137 GTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDH 196

Query: 198 LVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLS-----AVGVLY 257
           + GTK GG+IFD  GNRH A +LL  A+PK + V++ ATV RI+F+        A GV+Y
Sbjct: 197 INGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVY 256

Query: 258 SDSKGKLHTALIHKKG---EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVG 317
            D  G+ H A + K+G   EI LSAG +GSPQLL+LSG+GP + L +  + +V  QPHVG
Sbjct: 257 RDRTGQAHRAYL-KEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVG 316

Query: 318 QYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSL---------------ASPLPF 377
           Q M DNP  +  +  P  +  S  +VVG   +  ++++                +S   +
Sbjct: 317 QGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDY 376

Query: 378 FPL--PSYGLLPPQSTSITSSLVIIVGKFSNVSSKGWL---HLNNSSTDAKENPIVRFNY 437
           + +  P   LL   S +  SS     G F      G L   HL   + + K+NPIV FNY
Sbjct: 377 YAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNY 436

Query: 438 YSQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQF---MELPMPENLWN---- 497
           +    DL RCV G++ +E +++++   R K  D+     F++   +    P NL      
Sbjct: 437 FQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNLTASTPVNLRPPRSG 496

Query: 498 -----DSSIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTN 538
                  S EE+C++TV T WHYHGGC+VG+VVD +YKVIGI  LRV+D ST    PGTN
Sbjct: 497 PGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTN 556

BLAST of CsGy2G005940 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 237/556 (42.63%), Postives = 318/556 (57.19%), Query Frame = 0

Query: 30  SYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNT---YPFV 89
           S   F  N  D      YDYIVIGGGT GCPLA TLS  FSVL+LERG  P T     F+
Sbjct: 50  SSSSFSSNGQD----SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFL 109

Query: 90  LNEETLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAG 149
            N     +  + D    +  Q FVS DGV N R RVLGGGS +NAGFYSR    F + AG
Sbjct: 110 RN----FHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG 169

Query: 150 VDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGS 209
             WD +LV+++Y WVE  +V +P LT WQ A R++LLE GV P NGF   H+ GTKIGG+
Sbjct: 170 --WDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGT 229

Query: 210 IFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLS----AVGVLYSDSKGKLHTA 269
           IFD  G RH A ELL  ANP+ L+V+I ATVQ+I+F          GV++ D KG  H A
Sbjct: 230 IFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQA 289

Query: 270 LI--HKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSR 329
           L+   K  E+ LS+GAIGSPQ+L+LSGIGPK  L  LK+ +V    HVG+ M DNP  + 
Sbjct: 290 LLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTI 349

Query: 330 SIIFPFQLLASTAQVVGTLEKNIHLQSLA--SPLPFFPLPSYGLLP-------------- 389
            +     +  S  Q VG  +  +++++       P      YG++               
Sbjct: 350 LVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQR 409

Query: 390 -PQSTS--ITSSLV---------IIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQ 449
            P++T   IT +            I+ K +   S+G L L N++ D  +NP V FNY+  
Sbjct: 410 RPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKH 469

Query: 450 HGDLSRCVSGVRKVEDLLKT-----------QTMERIKTQDLEGNKGFQFMELPMPENLW 509
             DL RCV  +R V  ++ +           Q + ++ +  ++ N   +      P+ L 
Sbjct: 470 PVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLN 529

Query: 510 NDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMAT 538
           +  S+ ++CK+TV T WHYHGGCLVGKVV  N KV+G+  LRV+DGSTF +SPGTNP AT
Sbjct: 530 DTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQAT 587

BLAST of CsGy2G005940 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.0e-108
Identity = 233/574 (40.59%), Postives = 336/574 (58.54%), Query Frame = 0

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           M + I  F   IL+++ F+   +  + +    +F+ +A   P    +DYI+IGGGT GC 
Sbjct: 1   MNFHIFRFFQFILVAV-FIFHDSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCA 60

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LA TLS   +VL+LERG  P   P   +    + T +L+    +  Q F+SEDGV N R 
Sbjct: 61  LAATLSQNATVLVLERGGSPYDDPAATDIGNFANT-LLNITPNSWSQLFISEDGVFNSRA 120

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGG+++NAGFYSR  ++F   AG  W+ + VE AYEWVE+ +V  P +  WQ+AFR+
Sbjct: 121 RVLGGGTVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRD 180

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
            LLE GV P NGF   H+VGTK GG+IFD  G+RH A  LL  ANP  + V + A+V +I
Sbjct: 181 GLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKI 240

Query: 241 IF--KG---LSAVGVLYSDSKGKLHTALIHKK----GEIFLSAGAIGSPQLLLLSGIGPK 300
           +F  KG     A GV++ D+ G  + A +  +     E+ LSAGAI SPQLL+LSG+GP 
Sbjct: 241 LFTIKGNQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPA 300

Query: 301 SYLSSLKLH-LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQ---- 360
           ++L++ +++ ++  QP VGQ M DNP     I  P  +  S  Q VG  +   +++    
Sbjct: 301 AHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSA 360

Query: 361 ---SLASPLPFFP----LPSYGLLPPQSTS-----------ITSSLVIIVGKFSNVSSKG 420
              S++    FF     L     LP QS S           +T+   +I+ K +   S+G
Sbjct: 361 LSLSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRG 420

Query: 421 WLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKG 480
            L L N++ D  +NP V FNY+    DL++CV G+  +  ++ ++   + K   L   +G
Sbjct: 421 HLELRNTNPD--DNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARG 480

Query: 481 FQFMELPMPENL-----WNDSSIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLR 538
              + L +P NL      +   +E+YC +TV T +HYHGGC VGKVVD+NYKV+G+  LR
Sbjct: 481 LLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALR 540

BLAST of CsGy2G005940 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 386.0 bits (990), Expect = 5.1e-107
Identity = 230/553 (41.59%), Postives = 326/553 (58.95%), Query Frame = 0

Query: 31  YMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEE 90
           Y  F+ +A   P+  ++DYI+IGGGT+GC LA TLS   SVL+LERG  P   P   + E
Sbjct: 30  YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89

Query: 91  TLSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWD 150
             + T + +   ++  Q F+SEDGV N R RVLGGGS++NAGFY+R   E+ +    +W 
Sbjct: 90  NFA-TTLSNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE--TEWK 149

Query: 151 MELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDN 210
            + VE AYEWVE+ +  +P +  WQ AF++ LLE G  P NGF   H+ GTKIGG+IFD 
Sbjct: 150 TDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDR 209

Query: 211 KGNRHGAVELLNKANPKNLKVVIQATVQRIIF--KGL---SAVGVLYSDSKGKLHTALIH 270
            G+RH A +LL  ANP N+ V + A+V +I+F  KG     A GV++ D+ G LH A + 
Sbjct: 210 AGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELE 269

Query: 271 KK--GEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH-LVHHQPHVGQYMTDNPRFSRSI 330
           K    E+ LSAGAIGSPQLL+LSGIGP ++L++  +  LV   P VGQ M DNP  +  I
Sbjct: 270 KNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFI 329

Query: 331 IFPFQLLASTAQVVGTLEKNIHLQSLASPL-------PFFP-LPSY--GLLPPQSTSITS 390
             P  +  S  QVVG  +   +++  +  +        FF  + +Y   +   ++TS TS
Sbjct: 330 PSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTS 389

Query: 391 SLV-----------------------IIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNY 450
             +                       +I+ K +   S+G L L N++ D  +NP VRFNY
Sbjct: 390 PTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPD--DNPSVRFNY 449

Query: 451 YSQHGDLSRCVSGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENL-----WNDS 510
           Y +  DL  CV G+  +  ++ ++   + K  D   + G   + L +P NL      +  
Sbjct: 450 YQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIH-GLLDLMLSVPTNLRPRHITSMF 509

Query: 511 SIEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMM 538
           ++ ++C +TV T WHYHGGC VG+VVD NY+V+GI +LRV+DGSTF  SPGTNP AT+MM
Sbjct: 510 NLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMM 569

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527076.9e-14947.36(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
P527069.3e-14647.51(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
O242431.2e-14547.58(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Q945K27.9e-14546.96(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
O500482.3e-14447.05(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_011648923.10.099.63(R)-mandelonitrile lyase 3 [Cucumis sativus] >KGN61116.1 hypothetical protein Cs... [more]
XP_008451913.10.094.44PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo][more]
KAA0067297.10.094.96(R)-mandelonitrile lyase 3-like [Cucumis melo var. makuwa] >TYK10908.1 (R)-mande... [more]
XP_038889699.16.23e-30377.68(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_008452065.12.05e-29978.20PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0LMB80.099.63Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055040 PE=3 SV=1[more]
A0A1S3BTE40.094.44(R)-mandelonitrile lyase 3-like OS=Cucumis melo OX=3656 GN=LOC103493064 PE=3 SV=... [more]
A0A5D3CI610.094.96(R)-mandelonitrile lyase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BTS59.90e-30078.20(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=... [more]
A0A6J1HRM56.41e-29776.35(R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G73050.11.2e-14047.79Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.12.6e-11141.79Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.11.1e-10942.63Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.16.0e-10840.59Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.15.1e-10741.59Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 386..527
e-value: 2.4E-27
score: 96.2
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 47..316
e-value: 4.0E-32
score: 111.8
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 275..289
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 120..143
NoneNo IPR availableGENE3D3.30.410.40coord: 167..476
e-value: 6.6E-162
score: 541.8
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 5..538
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 5..538
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 367..483
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 47..526
e-value: 6.6E-162
score: 541.8
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 30..536
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 338..539
e-value: 1.9E-32
score: 110.7
coord: 26..342
e-value: 6.6E-55
score: 184.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G005940.2CsGy2G005940.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors