CsGy2G005906 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G005906
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description(R)-mandelonitrile lyase 1-like
LocationGy14Chr2: 4191016 .. 4193974 (-)
RNA-Seq ExpressionCsGy2G005906
SyntenyCsGy2G005906
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCACATAAAGAAATTTGAAAAAACACAATAATGGAGCATAGTCTTATTATGGCTTCCTTTCTTCTATTCATTCTCATATCTATGCTTCAGTTTCAATTGGGAGTCTCCCTCTCCTCAAACACTAAACCTAATGAAGGTAATAAAACCCCATTTTTCTTCCAACATCATTTAATTATGGTTGCCTCCTTGCATTGCTCGCTTTATCTTTGGGCTTGGAGCCTAATGAATATGCTTCCATGTCAACCTAATTGGGATGTCTCAACATATCTATTGATTTCTCTCATCGAGTGAATATTTTTTTAATTGCCTTTAATATTCTCATCATAAATATATTATTCCTTTTCTTTTCTTTTTTCCAGATTTTAGATACATGAAGTTTGTACATGATGCTAGTGATTTACCAACAAAAGAAAAATACGACTACATAATAATAGGGGGAGGAACAGCAGGGTGTCCATTAGCTGCAACATTAACATCAAATTTCTCAGTTCTACTTCTTGAAAGAGGTAGTGAACCAACCAAGTATCCCTCTGTATTGAAGGAACAAAGTTTCTTAAACGTCTACACGGTTGAAGACGATGGAGAAAATCCTTTCCAACGATTTGTTTCCGAGGATGGCGTGGAGAATCTAAGAGGACGAGTCCTTGGTGGTACTAGTATGCTCAATGGTGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCTGGAGTTAAGTGGGACATGGAATTGGTGAAAAAGGCTTATGAATGGGTTGAAGAGAGTGTGGTGTTTGAAGCAAGTTTGAATAATGGTTGGCAATATGCTTTCAGAAATGGTTTGTTGGAAGCTGGCGTTGGTCCTTATAATGGATTTGAGTTGAACCATCGTTTGGGGACTAAGATTGGAGGGTCAATCTTTGATAAGGAAGGAAATAGACATGGATCTGTGGAGCTTTTGAATAAGGCTCAACCCAATAATCTTAAAGTTGTCGTCCGAGCCACAGTAGAGAAAATCATCTTTTCTGGTTAGTGCTAAATGGAATAGTGAATCTCACATTATTTTAATTCATTGATATAACTATAACTATTTGGTTGTTGATAAATCCATTAATATTGATACGTAAAACTCGAAATCTTAATGTTGACAGTTTAGTCCTTATATTTAACACACTATTACATCCGGTTTGGGATTATATATGGTTCGGCTTTGGTTCACACCAATTCTTCTGCCGAATGAACGGTTCGGTTTTGGTTTTCTGTAAAAACCTAGTCGGACGGAACCACTTACACCCCTACTAGTTCCATAAATGGCTTCCTCCAACTTTGTATCACATTGATTTCTTTTCTTGGACTTGTGTAGGTTTATCAGCAAGTGGAGTTTTATATTCAGACTTCAAAGGAAGGTTACACACAGCATCCATCCGCAAAAAAGGAGAAATTATTCTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATCCATGTCTGACAATCCTCGTTTCACTGCCAATATAGTGCTTCCATATCCATTGGTTATCACAGCCGTAAAAGTTGTTGGAACCTTAGATGACAATATCCATCTTCAATCTATCACAGGATTTTTACCGTTTTCACTACCTCCATCATTTAGCCTTCTTCCTCATAGATTTAATTCCGTCAATTTGAGCTTAGCCACCATCGTTGGAAAATTCTCTGAGGTTTTTTCCGAAGGGTCCTTGAAATTGAATTCTTCAACTGATGTGAAGAAAAATCCCATTGTTAGATTTAATTACTATTCTCATCCTGACGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAATTTGGAGATTTTCTTAAAACTCCAACAATTGAAAAGATTAAGATACAAGATTTTGAGGGTAAGAGAAGTTTTGCGTTTTTGGAACCTCCGATACCGGAAAATTTATCGGATGATGGTGTGGTTGAAAAGTTTTGTAAGGAAACAGTGACGACTTATTGGCATTACCATGGAGGATGCTTGGTAGGAAAAGTTGTCGACGGCAATTATAGAGTCATGGGAATTAAAAATTTGCGTGTAGTAGATGGCTCCACGTTTTCTGATTCACCTGGAACCAATCCTATGGCTACTCTCATGATGCTTGGCCGGTGAGTTTTATATTTATATGTATGTTTGTTTGACTATATATGTTTTCAATTTGTTGTCCAAATAAACAAAGTGGATTCGAAATATTGTTTATAACATAATTTTGAACAAATCACATGTTTATCAAACAATATTTTAATTATTTAAGCTTTGTTGATAACAATTTGATTTTGAATTTTTGTTTTTTTAAATATTAAATTCGTAGACTTCTAAATTTAGTATTTTGTTATCTTTTTTTTTCCCATGATTTAAAAACCAAAGCAAATTTTGAAAACTAAGAAAAAGGGTTTCTAGACACTTTTTTTATTTATTTTGAATTTAATTAAAAATTAACTAGTGTACTTACAAATGTAGATCACGAAAAAAAATAGATTTAATTTTCAAATACAAAAAAAAGAAAAATATGTTATTGAGCAAAATGTTTAAATAAACTACACTTTGATATTTTTTTCTTCAAAAAGAAGTTAGTGCAGCTTTTAGTTTAACAATATATGGAGTTGAAATTTGATTCTTTAATTTGGTACTTTGTACTCAAAGACGATATAGATTTTTAAAAAGTTAAATTATGGTTTAGATTATTTTATTGCCCTTTACTCTATCTAATTCTGGCAATTTTTTAATTGTTTTGCAGATATGTTGGGCTTAATATATTGCGAGAAAGATCAAGTTAAAAGTGATTAAACTATTCTAATTAGGCCATTTCATAACACATTCTTGTTTATGTGGTCTTGTATCCTATGTTTAAATTCTAATGAATAAATGTGGTT

mRNA sequence

CTCCACATAAAGAAATTTGAAAAAACACAATAATGGAGCATAGTCTTATTATGGCTTCCTTTCTTCTATTCATTCTCATATCTATGCTTCAGTTTCAATTGGGAGTCTCCCTCTCCTCAAACACTAAACCTAATGAAGATTTTAGATACATGAAGTTTGTACATGATGCTAGTGATTTACCAACAAAAGAAAAATACGACTACATAATAATAGGGGGAGGAACAGCAGGGTGTCCATTAGCTGCAACATTAACATCAAATTTCTCAGTTCTACTTCTTGAAAGAGGTAGTGAACCAACCAAGTATCCCTCTGTATTGAAGGAACAAAGTTTCTTAAACGTCTACACGGTTGAAGACGATGGAGAAAATCCTTTCCAACGATTTGTTTCCGAGGATGGCGTGGAGAATCTAAGAGGACGAGTCCTTGGTGGTACTAGTATGCTCAATGGTGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCTGGAGTTAAGTGGGACATGGAATTGGTGAAAAAGGCTTATGAATGGGTTGAAGAGAGTGTGGTGTTTGAAGCAAGTTTGAATAATGGTTGGCAATATGCTTTCAGAAATGGTTTGTTGGAAGCTGGCGTTGGTCCTTATAATGGATTTGAGTTGAACCATCGTTTGGGGACTAAGATTGGAGGGTCAATCTTTGATAAGGAAGGAAATAGACATGGATCTGTGGAGCTTTTGAATAAGGCTCAACCCAATAATCTTAAAGTTGTCGTCCGAGCCACAGTAGAGAAAATCATCTTTTCTGGTTTATCAGCAAGTGGAGTTTTATATTCAGACTTCAAAGGAAGGTTACACACAGCATCCATCCGCAAAAAAGGAGAAATTATTCTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATCCATGTCTGACAATCCTCGTTTCACTGCCAATATAGTGCTTCCATATCCATTGGTTATCACAGCCGTAAAAGTTGTTGGAACCTTAGATGACAATATCCATCTTCAATCTATCACAGGATTTTTACCGTTTTCACTACCTCCATCATTTAGCCTTCTTCCTCATAGATTTAATTCCGTCAATTTGAGCTTAGCCACCATCGTTGGAAAATTCTCTGAGGTTTTTTCCGAAGGGTCCTTGAAATTGAATTCTTCAACTGATGTGAAGAAAAATCCCATTGTTAGATTTAATTACTATTCTCATCCTGACGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAATTTGGAGATTTTCTTAAAACTCCAACAATTGAAAAGATTAAGATACAAGATTTTGAGGGTAAGAGAAGTTTTGCGTTTTTGGAACCTCCGATACCGGAAAATTTATCGGATGATGGTGTGGTTGAAAAGTTTTGTAAGGAAACAGTGACGACTTATTGGCATTACCATGGAGGATGCTTGGTAGGAAAAGTTGTCGACGGCAATTATAGAGTCATGGGAATTAAAAATTTGCGTGTAGTAGATGGCTCCACGTTTTCTGATTCACCTGGAACCAATCCTATGGCTACTCTCATGATGCTTGGCCGATATGTTGGGCTTAATATATTGCGAGAAAGATCAAGTTAAAAGTGATTAAACTATTCTAATTAGGCCATTTCATAACACATTCTTGTTTATGTGGTCTTGTATCCTATGTTTAAATTCTAATGAATAAATGTGGTT

Coding sequence (CDS)

ATGGAGCATAGTCTTATTATGGCTTCCTTTCTTCTATTCATTCTCATATCTATGCTTCAGTTTCAATTGGGAGTCTCCCTCTCCTCAAACACTAAACCTAATGAAGATTTTAGATACATGAAGTTTGTACATGATGCTAGTGATTTACCAACAAAAGAAAAATACGACTACATAATAATAGGGGGAGGAACAGCAGGGTGTCCATTAGCTGCAACATTAACATCAAATTTCTCAGTTCTACTTCTTGAAAGAGGTAGTGAACCAACCAAGTATCCCTCTGTATTGAAGGAACAAAGTTTCTTAAACGTCTACACGGTTGAAGACGATGGAGAAAATCCTTTCCAACGATTTGTTTCCGAGGATGGCGTGGAGAATCTAAGAGGACGAGTCCTTGGTGGTACTAGTATGCTCAATGGTGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCTGGAGTTAAGTGGGACATGGAATTGGTGAAAAAGGCTTATGAATGGGTTGAAGAGAGTGTGGTGTTTGAAGCAAGTTTGAATAATGGTTGGCAATATGCTTTCAGAAATGGTTTGTTGGAAGCTGGCGTTGGTCCTTATAATGGATTTGAGTTGAACCATCGTTTGGGGACTAAGATTGGAGGGTCAATCTTTGATAAGGAAGGAAATAGACATGGATCTGTGGAGCTTTTGAATAAGGCTCAACCCAATAATCTTAAAGTTGTCGTCCGAGCCACAGTAGAGAAAATCATCTTTTCTGGTTTATCAGCAAGTGGAGTTTTATATTCAGACTTCAAAGGAAGGTTACACACAGCATCCATCCGCAAAAAAGGAGAAATTATTCTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATCCATGTCTGACAATCCTCGTTTCACTGCCAATATAGTGCTTCCATATCCATTGGTTATCACAGCCGTAAAAGTTGTTGGAACCTTAGATGACAATATCCATCTTCAATCTATCACAGGATTTTTACCGTTTTCACTACCTCCATCATTTAGCCTTCTTCCTCATAGATTTAATTCCGTCAATTTGAGCTTAGCCACCATCGTTGGAAAATTCTCTGAGGTTTTTTCCGAAGGGTCCTTGAAATTGAATTCTTCAACTGATGTGAAGAAAAATCCCATTGTTAGATTTAATTACTATTCTCATCCTGACGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAATTTGGAGATTTTCTTAAAACTCCAACAATTGAAAAGATTAAGATACAAGATTTTGAGGGTAAGAGAAGTTTTGCGTTTTTGGAACCTCCGATACCGGAAAATTTATCGGATGATGGTGTGGTTGAAAAGTTTTGTAAGGAAACAGTGACGACTTATTGGCATTACCATGGAGGATGCTTGGTAGGAAAAGTTGTCGACGGCAATTATAGAGTCATGGGAATTAAAAATTTGCGTGTAGTAGATGGCTCCACGTTTTCTGATTCACCTGGAACCAATCCTATGGCTACTCTCATGATGCTTGGCCGATATGTTGGGCTTAATATATTGCGAGAAAGATCAAGTTAA

Protein sequence

MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS*
Homology
BLAST of CsGy2G005906 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.5e-154
Identity = 278/545 (51.01%), Postives = 370/545 (67.89%), Query Frame = 0

Query: 7   MASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 66
           M++ LL + + +L  Q     S  T  N DF Y++F +DA+DL  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 67  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 126
           CPLAATL+  + VL+LERGS PT YP+VL    F+     EDDG+ P +RFVSEDG++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 127 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 186
           RGRVLGGTSM+N G Y+R +   +  +GV WDM+LV K YEWVE+++VF+ +    WQ  
Sbjct: 126 RGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNY-QPWQSV 185

Query: 187 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 246
                LEAGV P +GF L+H  GT+I GS FD +G RH + ELLNK   NNL+V V A+V
Sbjct: 186 TGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 245

Query: 247 EKIIFS---GLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHL 306
           EKIIFS   GL+A+GV+Y D  G  H A +R KGE+I+SAG IG+PQLLLLSGVGP+S+L
Sbjct: 246 EKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 305

Query: 307 SSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPF 366
           SSL +PVVL  P+VGQ + DNPR   NI+ P P+  T V V+G  +D    Q     LPF
Sbjct: 306 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPF 365

Query: 367 SLPPSFSLLPH-RFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPD 426
           + PP FS  P   +   N + A    K +   S GSL L SS++V+ +P V+FNYYS+P 
Sbjct: 366 TTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPT 425

Query: 427 DLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETV 486
           DL+ CV G++K G+ L T  ++  K++D  G   F  L  P+P++ +DD   E FC+E+V
Sbjct: 426 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESV 485

Query: 487 TTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNIL 546
            +YWHYHGGCLVGKV+DG++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+ IL
Sbjct: 486 ASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKIL 545

Query: 547 RERSS 548
           +ERS+
Sbjct: 546 QERSA 546

BLAST of CsGy2G005906 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.5e-154
Identity = 279/546 (51.10%), Postives = 366/546 (67.03%), Query Frame = 0

Query: 7   MASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 66
           M++ LL + I +L  Q     S     + DF Y+ FV+DA+D   +  YDYII+GGGTAG
Sbjct: 6   MSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAG 65

Query: 67  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 126
           CPLAATL++N+SVL+LERGS PT+YP++L    F+     EDDG+ P +RFVSEDG++N+
Sbjct: 66  CPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 127 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 186
           RGRVLGGTSM+N G Y R +  FF   G++WDM+LV + YEWVE+++VFE   +  WQ  
Sbjct: 126 RGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPD-SQTWQTV 185

Query: 187 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 246
                LEAG+ P NGF ++H  GT++ GS FD  G RH S ELLNK  PNNL+V V+A V
Sbjct: 186 IGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAV 245

Query: 247 EKIIF----SGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSH 306
           EKIIF    SG++A GV+Y+D  G  H A +R +GE+ILSAG IGSPQLLLLSGVGP+S+
Sbjct: 246 EKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESY 305

Query: 307 LSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLP 366
           L+SL + VV   P+VGQ + DNPR   NI+ P P+  + V V+G   D    Q     LP
Sbjct: 306 LTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLP 365

Query: 367 FSLPPSFSLLP-HRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHP 426
           F  PP FS  P   +   N + A IV K     S G++ LNSS+DV+  P V+FNYYS+ 
Sbjct: 366 FDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNL 425

Query: 427 DDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKET 486
            DL+ CV G++K G+ L T  +E  K++D  G   F  L  P+PEN +DD   E FC+E+
Sbjct: 426 TDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRES 485

Query: 487 VTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNI 546
           V +YWHYHGGCLVGKV+D  +RV GI  LRVVDGSTF  +P ++P    +MLGRY+G+ I
Sbjct: 486 VASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQI 545

Query: 547 LRERSS 548
           L+ERS+
Sbjct: 546 LQERSA 547

BLAST of CsGy2G005906 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 4.0e-152
Identity = 273/545 (50.09%), Postives = 369/545 (67.71%), Query Frame = 0

Query: 7   MASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 66
           M++ LL + I +L  Q     S  T  + DF Y+ F +DA+DL  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 67  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 126
           CPLAATL+  + VL+LERGS PT YP+VL    F+     EDDG+ P +RFVSEDG++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 127 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 186
           RGRVLGGTS++N G Y+R +   +  +GV WDM+LV + YEWVE+++V++ + +  WQ  
Sbjct: 126 RGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPN-SQSWQSV 185

Query: 187 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 246
            +   LEAGV P +GF L+H  GT+I GS FD +G RH + ELLNK   NNL+V V A+V
Sbjct: 186 TKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 245

Query: 247 EKIIFS---GLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHL 306
           EKIIFS   GL+A+GV+Y D  G  H A +R KGE+I+SAG IG+PQLLLLSGVGP+S+L
Sbjct: 246 EKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 305

Query: 307 SSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPF 366
           SSL +PVVL  P+VGQ + DNPR   NI+ P P+  T V V+G  +D    Q     LPF
Sbjct: 306 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPF 365

Query: 367 SLPPSFSLLPH-RFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPD 426
           + PP F   P   +   N + A    K +   S GSL L SS++V+ +P V+FNYYS+  
Sbjct: 366 TTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLT 425

Query: 427 DLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETV 486
           DL+ CV G++K G+ L T  ++  K++D  G   F  L  P+P++ +DD   E FC+E+V
Sbjct: 426 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESV 485

Query: 487 TTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNIL 546
            +YWHYHGGCLVGKV+DG++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+ IL
Sbjct: 486 ASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKIL 545

Query: 547 RERSS 548
           +ERS+
Sbjct: 546 QERSA 546

BLAST of CsGy2G005906 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.2e-150
Identity = 280/553 (50.63%), Postives = 369/553 (66.73%), Query Frame = 0

Query: 1   MEHSLIMASFL-LFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYII 60
           ME S + A  L L +L+  LQ+    SL +NT    DF Y+KFV++A DL  +  YDYII
Sbjct: 1   MEKSTMSAVVLVLNLLVLHLQYSEVHSL-ANTSSEHDFGYLKFVYNAVDLELEGSYDYII 60

Query: 61  IGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVS 120
           +GGGT+GCPLAATL++N+SVL+LERG+  T+YP+ L    F      +DDG+ P +RFVS
Sbjct: 61  VGGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVS 120

Query: 121 EDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASL 180
           EDG++N+R R+LGGT+++N G Y+R ++ F+  +GV+WD++LV +AYEWVE+++V++ S 
Sbjct: 121 EDGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPS- 180

Query: 181 NNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLK 240
           N  WQ       LEAGV P NGF L H  GT++ GS FD  G RH S ELLNK  P+NLK
Sbjct: 181 NQSWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLK 240

Query: 241 VVVRATVEKIIF----SGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLS 300
           V V A V+KIIF    SGL+A GV+Y+D  G  H A +  KGE+ILSAG +G+PQLLLLS
Sbjct: 241 VAVEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLS 300

Query: 301 GVGPKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQ 360
           GVGP+S+L+SL + VV   P+VGQ ++DNPR   NI+ P P+  + V V+G   D    Q
Sbjct: 301 GVGPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQ 360

Query: 361 SITGFLPFSLPPSFSLLP-HRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVR 420
                LPF  PP FSL P   +   N + A IV K     S GSL L SS++V   P V+
Sbjct: 361 CSLSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVK 420

Query: 421 FNYYSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVV 480
           FNY S P DL  CV G++K G FL T  ++  K+ D  G   F  L  P+PEN +DD   
Sbjct: 421 FNYCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAF 480

Query: 481 EKFCKETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLG 540
           EKFC++TV +YWHYHGG +VGKV+DGN+RV GI  LRVVDGSTF  +P ++P    +MLG
Sbjct: 481 EKFCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLG 540

Query: 541 RYVGLNILRERSS 548
           RYVG  I++ERS+
Sbjct: 541 RYVGTKIVQERSA 548

BLAST of CsGy2G005906 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 5.3e-149
Identity = 268/547 (48.99%), Postives = 374/547 (68.37%), Query Frame = 0

Query: 9   SFLLFILISMLQFQLGVSLSSNTKP--NEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 68
           S LL+  + +L   LGV   SN +P  N    +M+F+ +A+D  +++ YDYII+GGGTAG
Sbjct: 7   SSLLYTALVVL-LLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAG 66

Query: 69  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 128
           CPLAATL+ +F VLLLERG  P   P+V+    FL   T  ++ ++P Q F+SE+GV N 
Sbjct: 67  CPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNA 126

Query: 129 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 188
           RGRVLGG+S +N GFYSR  ++FFE +G+ WD+  V ++YEWVE ++VF   L   WQ A
Sbjct: 127 RGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRT-WQTA 186

Query: 189 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 248
            R+ LLE GV P+NGF L H++GTKIGGS FD+ G RH S +LL  A+ +N++V V ATV
Sbjct: 187 IRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATV 246

Query: 249 EKIIF--------SGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVG 308
           E+++         S +SA GV+Y D  GR H A IR +GE+ILSAGA+GSPQLL LSG+G
Sbjct: 247 ERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIG 306

Query: 309 PKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSIT 368
           P+S+LS+  +PV L QPHVG  + DNPR   +IV P P+  + ++VVG  +D   L++ +
Sbjct: 307 PRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAAS 366

Query: 369 GFLPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYY 428
             +PF+ P     +    + + + + TI+ K     S G L+L +STDV+ NP+VRFNY+
Sbjct: 367 NVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYF 426

Query: 429 SHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFC 488
           S P DL +CV G RK G+ L++  ++   I+++ G R F F+  P+P + S+D V+  FC
Sbjct: 427 SDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFC 486

Query: 489 KETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVG 546
           + TV+T WHYHGG +VGKVVD + +V+G+ +LR+VDGSTF+ SPGTNP ATLMMLGRY+G
Sbjct: 487 RRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMG 546

BLAST of CsGy2G005906 vs. NCBI nr
Match: XP_031736342.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical protein Csa_021315 [Cucumis sativus])

HSP 1 Score: 1090 bits (2820), Expect = 0.0
Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 0

Query: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60
           MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII
Sbjct: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60

Query: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120
           GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE
Sbjct: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120

Query: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180
           DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN
Sbjct: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180

Query: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240
           NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV
Sbjct: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240

Query: 241 VVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300
           VVRATVEKIIFSGLSASGVLYSD KGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK
Sbjct: 241 VVRATVEKIIFSGLSASGVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300

Query: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360
           SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF
Sbjct: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360

Query: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420
           LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH
Sbjct: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420

Query: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480
           PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE
Sbjct: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540
           TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN
Sbjct: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540

Query: 541 ILRERSS 547
           ILRERSS
Sbjct: 541 ILRERSS 547

BLAST of CsGy2G005906 vs. NCBI nr
Match: XP_031736419.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 1089 bits (2816), Expect = 0.0
Identity = 545/547 (99.63%), Postives = 546/547 (99.82%), Query Frame = 0

Query: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60
           MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII
Sbjct: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60

Query: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120
           GGGTAGCPLAATL+SNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE
Sbjct: 61  GGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120

Query: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180
           DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN
Sbjct: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180

Query: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240
           NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV
Sbjct: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240

Query: 241 VVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300
           VVRATVEKIIFSGLSASGVLYSD KGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK
Sbjct: 241 VVRATVEKIIFSGLSASGVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300

Query: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360
           SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF
Sbjct: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360

Query: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420
           LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH
Sbjct: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420

Query: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480
           PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE
Sbjct: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540
           TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN
Sbjct: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540

Query: 541 ILRERSS 547
           ILRERSS
Sbjct: 541 ILRERSS 547

BLAST of CsGy2G005906 vs. NCBI nr
Match: XP_008451933.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 1010 bits (2611), Expect = 0.0
Identity = 502/535 (93.83%), Postives = 517/535 (96.64%), Query Frame = 0

Query: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60
           MEHSLIMASF LFILISML F LG  LSSNT PNEDFRYMKFVHDASDLPT+E+YDYIII
Sbjct: 14  MEHSLIMASFPLFILISMLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIII 73

Query: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120
           GGGTAGCPLAATL+SNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE
Sbjct: 74  GGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 133

Query: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180
           DGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAGVKWDMELVKKAYEWVEE VVFEASLN
Sbjct: 134 DGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLN 193

Query: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240
           NGWQYAFRNGLLEAGVGPYN F+LNHRLGTKIGGSIFDKEGNRHGSVELLNKA PNNLKV
Sbjct: 194 NGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKV 253

Query: 241 VVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300
           VVRATVEKIIFSGLSA GVLYSD KG+LHTASIR+KGEIILSAGAIGSPQLLLLSGVGPK
Sbjct: 254 VVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPK 313

Query: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360
           SHLSSLKLP+VLHQPHVGQSMSDNPRFT N+VLPYPLVITAVKVVGTLDDNIHLQSITGF
Sbjct: 314 SHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGF 373

Query: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420
           LPFSLPPSFSLLP  F+SVNLSLAT+VGKFSEV SEGSLKLNSSTDVKKNPIVRFNYYSH
Sbjct: 374 LPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSH 433

Query: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480
           PDDLAKCVRGVRK GDFLKT TIEKIKIQDFEGKRSFAFLEPP+PENLSD GVVEK+CK+
Sbjct: 434 PDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKK 493

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 535
           TVTTYWHYHGGCLVGKVVDGNYRVMG+KNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 494 TVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

BLAST of CsGy2G005906 vs. NCBI nr
Match: KAA0067300.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10911.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 469/496 (94.56%), Postives = 484/496 (97.58%), Query Frame = 0

Query: 40  MKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQS 99
           MKFVHDASDLPT+E+YDYIIIGGGTAGCPLAATL+SNFSVLLLERGSEPTKYPSVLKEQS
Sbjct: 1   MKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQS 60

Query: 100 FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDM 159
           FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAGVKWDM
Sbjct: 61  FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDM 120

Query: 160 ELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDK 219
           ELVKKAYEWVEE VVFEASLNNGWQYAFRNGLLEAGVGPYN F+LNHRLGTKIGGSIFDK
Sbjct: 121 ELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDK 180

Query: 220 EGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEI 279
           EGNRHGSVELLNKA PNNLKVVVRATVEKIIFSGLSA GVLYSD KG+LHTASIR+KGEI
Sbjct: 181 EGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEI 240

Query: 280 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVI 339
           ILSAGAIGSPQLLLLSGVGPKSHLSSLKLP+VLHQPHVGQSMSDNPRFT N+VLPYPLVI
Sbjct: 241 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVI 300

Query: 340 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSL 399
           TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLP  F+SVNLSLAT+VGKFSEV SEGSL
Sbjct: 301 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSL 360

Query: 400 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAF 459
           KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK GDFLKT TIEKIKIQDFEGKRSFAF
Sbjct: 361 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAF 420

Query: 460 LEPPIPENLSDDGVVEKFCKETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFS 519
           LEPP+PENLSD GVVEK+CK+TVTTYWHYHGGCLVGKVVDGNYRVMG+KNLRVVDGSTFS
Sbjct: 421 LEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFS 480

Query: 520 DSPGTNPMATLMMLGR 535
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of CsGy2G005906 vs. NCBI nr
Match: XP_038891172.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 456/548 (83.21%), Postives = 504/548 (91.97%), Query Frame = 0

Query: 1   MEHSLIMASFLLFILISML-QFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYII 60
           MEH   MA+FLLF L+SML  FQLGVSLSSNT PNED RYMKFVHDAS+LP KE+YDYII
Sbjct: 1   MEHRRPMAAFLLFFLVSMLLHFQLGVSLSSNTNPNEDVRYMKFVHDASELPKKEEYDYII 60

Query: 61  IGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVS 120
           IGGGTAGCPLAATL+SNFSVLLLERGSEPTKYP+VL EQ+F NV+T+EDDG+NPFQRFVS
Sbjct: 61  IGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPTVLSEQTFTNVFTIEDDGKNPFQRFVS 120

Query: 121 EDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASL 180
           EDGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAGV WDM+LV+KAYEWVEESVVF+ASL
Sbjct: 121 EDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVNWDMKLVEKAYEWVEESVVFQASL 180

Query: 181 NNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLK 240
           NNGWQYAFR GLLEAGVGP NGF+L H +GTKIGGSIFDKEGNRHG+VELLNKA+PNNLK
Sbjct: 181 NNGWQYAFRKGLLEAGVGPDNGFDLRHSVGTKIGGSIFDKEGNRHGAVELLNKAEPNNLK 240

Query: 241 VVVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGP 300
           V ++ATV+KI+FSGLSASGV YSD KG+LH A +RKKGEIILSAGAIGSPQLLLLSGVGP
Sbjct: 241 VAIQATVQKILFSGLSASGVSYSDSKGKLHRAFVRKKGEIILSAGAIGSPQLLLLSGVGP 300

Query: 301 KSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITG 360
           KSHLSSLK+PVVL+Q HVGQ MSDNPR+T NI+LP+PLV TAVKVVGTL++NIHLQSIT 
Sbjct: 301 KSHLSSLKVPVVLNQHHVGQFMSDNPRYTVNILLPFPLVTTAVKVVGTLENNIHLQSITS 360

Query: 361 FLPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYS 420
           +LPFS+P SFSL+PH F+SVNLSLATI GKFSEV S+G L L +STDVKK+P VRFNYYS
Sbjct: 361 YLPFSVPLSFSLIPHGFDSVNLSLATIAGKFSEVLSDGYLHL-TSTDVKKSPTVRFNYYS 420

Query: 421 HPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCK 480
           HPDDLAKCVRGVRK GDFLKT TIE IKIQDFEG++ F FL P +P+NLSD   VE++CK
Sbjct: 421 HPDDLAKCVRGVRKIGDFLKTQTIENIKIQDFEGRKGFEFLGPRLPQNLSDVSSVEEYCK 480

Query: 481 ETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGL 540
           +TVTTYWHYHGGCLVGKVVDGNYRV+GIKNLRVVDGSTFS+SPGTNPMATL+MLGRYVGL
Sbjct: 481 KTVTTYWHYHGGCLVGKVVDGNYRVIGIKNLRVVDGSTFSESPGTNPMATLLMLGRYVGL 540

Query: 541 NILRERSS 547
           NILRERSS
Sbjct: 541 NILRERSS 547

BLAST of CsGy2G005906 vs. ExPASy TrEMBL
Match: A0A1S3BS25 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=1)

HSP 1 Score: 1010 bits (2611), Expect = 0.0
Identity = 502/535 (93.83%), Postives = 517/535 (96.64%), Query Frame = 0

Query: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60
           MEHSLIMASF LFILISML F LG  LSSNT PNEDFRYMKFVHDASDLPT+E+YDYIII
Sbjct: 14  MEHSLIMASFPLFILISMLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIII 73

Query: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120
           GGGTAGCPLAATL+SNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE
Sbjct: 74  GGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 133

Query: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180
           DGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAGVKWDMELVKKAYEWVEE VVFEASLN
Sbjct: 134 DGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLN 193

Query: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240
           NGWQYAFRNGLLEAGVGPYN F+LNHRLGTKIGGSIFDKEGNRHGSVELLNKA PNNLKV
Sbjct: 194 NGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKV 253

Query: 241 VVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300
           VVRATVEKIIFSGLSA GVLYSD KG+LHTASIR+KGEIILSAGAIGSPQLLLLSGVGPK
Sbjct: 254 VVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPK 313

Query: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360
           SHLSSLKLP+VLHQPHVGQSMSDNPRFT N+VLPYPLVITAVKVVGTLDDNIHLQSITGF
Sbjct: 314 SHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGF 373

Query: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420
           LPFSLPPSFSLLP  F+SVNLSLAT+VGKFSEV SEGSLKLNSSTDVKKNPIVRFNYYSH
Sbjct: 374 LPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSH 433

Query: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480
           PDDLAKCVRGVRK GDFLKT TIEKIKIQDFEGKRSFAFLEPP+PENLSD GVVEK+CK+
Sbjct: 434 PDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKK 493

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 535
           TVTTYWHYHGGCLVGKVVDGNYRVMG+KNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 494 TVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

BLAST of CsGy2G005906 vs. ExPASy TrEMBL
Match: A0A5A7VJA1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00920 PE=3 SV=1)

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 469/496 (94.56%), Postives = 484/496 (97.58%), Query Frame = 0

Query: 40  MKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQS 99
           MKFVHDASDLPT+E+YDYIIIGGGTAGCPLAATL+SNFSVLLLERGSEPTKYPSVLKEQS
Sbjct: 1   MKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQS 60

Query: 100 FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDM 159
           FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAGVKWDM
Sbjct: 61  FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDM 120

Query: 160 ELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDK 219
           ELVKKAYEWVEE VVFEASLNNGWQYAFRNGLLEAGVGPYN F+LNHRLGTKIGGSIFDK
Sbjct: 121 ELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDK 180

Query: 220 EGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEI 279
           EGNRHGSVELLNKA PNNLKVVVRATVEKIIFSGLSA GVLYSD KG+LHTASIR+KGEI
Sbjct: 181 EGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEI 240

Query: 280 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVI 339
           ILSAGAIGSPQLLLLSGVGPKSHLSSLKLP+VLHQPHVGQSMSDNPRFT N+VLPYPLVI
Sbjct: 241 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVI 300

Query: 340 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSL 399
           TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLP  F+SVNLSLAT+VGKFSEV SEGSL
Sbjct: 301 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSL 360

Query: 400 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAF 459
           KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK GDFLKT TIEKIKIQDFEGKRSFAF
Sbjct: 361 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAF 420

Query: 460 LEPPIPENLSDDGVVEKFCKETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFS 519
           LEPP+PENLSD GVVEK+CK+TVTTYWHYHGGCLVGKVVDGNYRVMG+KNLRVVDGSTFS
Sbjct: 421 LEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFS 480

Query: 520 DSPGTNPMATLMMLGR 535
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

BLAST of CsGy2G005906 vs. ExPASy TrEMBL
Match: A0A1S3BTD3 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493053 PE=3 SV=1)

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 428/541 (79.11%), Postives = 483/541 (89.28%), Query Frame = 0

Query: 7   MASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 66
           MASFLLFIL+S L FQ G  LSSNT  NEDF+YM FV DAS+LP  E+YDYI+IGGGTAG
Sbjct: 1   MASFLLFILMSTLHFQFGFPLSSNTNSNEDFKYMNFVQDASELPENEEYDYIVIGGGTAG 60

Query: 67  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 126
           CPLA TL+S FSVLLLERG+ PTKYP+VL+E++F N +T+EDDGENPFQRFVSEDGVE +
Sbjct: 61  CPLATTLSSTFSVLLLERGNVPTKYPTVLREETFPNAFTIEDDGENPFQRFVSEDGVEII 120

Query: 127 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 186
           RGRVLGG+SMLN GFYSRGHQEFF+ +GV WDMELV+KAYEWVEESVVFEA++NNGWQ A
Sbjct: 121 RGRVLGGSSMLNAGFYSRGHQEFFDISGVDWDMELVEKAYEWVEESVVFEANINNGWQNA 180

Query: 187 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 246
           FR  LLEAGVGPYNGF+LNHR+GTKIGGSIFDKEGNRHGS+ELLNKA PNNLKV ++ATV
Sbjct: 181 FRKALLEAGVGPYNGFDLNHRIGTKIGGSIFDKEGNRHGSLELLNKAHPNNLKVAIQATV 240

Query: 247 EKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 306
           +KI+FS LSA+GV YSD KG LHTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL
Sbjct: 241 QKILFSDLSATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESL 300

Query: 307 KLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLP 366
           +LPVVL QPHVGQSMSDNPR+  +++LPY L  +AVK VGTL++N+HLQSITGFLPFSLP
Sbjct: 301 ELPVVLDQPHVGQSMSDNPRYNVHVILPYQLATSAVKTVGTLENNVHLQSITGFLPFSLP 360

Query: 367 PSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAK 426
           PSFSL+P   +SVNLSLA+IVGKFSEV SEGSL L SSTDVKKNP V FNYYSHPDDLAK
Sbjct: 361 PSFSLVPPGLDSVNLSLASIVGKFSEVLSEGSLYLTSSTDVKKNPSVSFNYYSHPDDLAK 420

Query: 427 CVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYW 486
           CVRGVRK G+ LKT  +EKIKIQDFEG + FAF+EP +PENLSD G VE+FCK+TVTTYW
Sbjct: 421 CVRGVRKMGELLKTEAMEKIKIQDFEGNKRFAFVEPSLPENLSDVGSVEEFCKKTVTTYW 480

Query: 487 HYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERS 546
           HYHGGCLVGKVVDGNY+V+GI+NLRVVDGSTF DSPGTNPMAT+MMLGRYVGLNI++ERS
Sbjct: 481 HYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFIDSPGTNPMATVMMLGRYVGLNIIQERS 540

BLAST of CsGy2G005906 vs. ExPASy TrEMBL
Match: A0A0A0LJ83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055560 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 429/547 (78.43%), Postives = 487/547 (89.03%), Query Frame = 0

Query: 1   MEHSLIMASFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIII 60
           MEH+L MASFLLFIL+SML FQ G  LSSN+ PNED +YM FV DAS+L   E+YDYI+I
Sbjct: 1   MEHNL-MASFLLFILMSMLHFQFGFPLSSNSNPNEDLKYMNFVQDASELLENEEYDYIVI 60

Query: 61  GGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSE 120
           GGGTAGCPLA TL++NFSVLLLERG+ PTKYP+VL EQ+F NV+T EDDGENPFQRFVSE
Sbjct: 61  GGGTAGCPLATTLSANFSVLLLERGNVPTKYPTVLSEQAFPNVFTTEDDGENPFQRFVSE 120

Query: 121 DGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLN 180
           DGVE +RGRVLGG+SMLN GFYSRGH EFF+ +GV WD ELV+KAYEWVEESV+FEA++N
Sbjct: 121 DGVEIIRGRVLGGSSMLNAGFYSRGHPEFFDISGVDWDKELVEKAYEWVEESVIFEANIN 180

Query: 181 NGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240
           NGWQYAFR  LLEAGVGPY+GF+LNHR+GTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV
Sbjct: 181 NGWQYAFRKALLEAGVGPYHGFDLNHRIGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKV 240

Query: 241 VVRATVEKIIFSGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPK 300
            ++A V+KI+FS LSA+GV YSD KG LHTASIRK GEII+SAG IGSPQLLLLSG+GPK
Sbjct: 241 GIQAVVQKILFSDLSATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPK 300

Query: 301 SHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGF 360
           SHL SLK+PVVLHQP+VGQSMSDNPR+T +++LPYP+  +AVK VGTL++N+HLQSITGF
Sbjct: 301 SHLESLKIPVVLHQPYVGQSMSDNPRYTVHVILPYPMATSAVKAVGTLENNVHLQSITGF 360

Query: 361 LPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSH 420
           LPFSLPPSFSL+P   +SVNLSLA++VGKFSEV SEGSL L SSTDVK+NP V FNYYSH
Sbjct: 361 LPFSLPPSFSLVPPGLDSVNLSLASLVGKFSEVLSEGSLYLTSSTDVKENPSVSFNYYSH 420

Query: 421 PDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKE 480
           PDDLAKCVRGVRK GD LKT  +E IKIQDFEG + FAFLEP +P NLSD G+VE+FCK+
Sbjct: 421 PDDLAKCVRGVRKMGDLLKTEAMENIKIQDFEGNKRFAFLEPSLPGNLSDVGLVEEFCKK 480

Query: 481 TVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLN 540
           TVTTYWHYHGGCLVGKVVDGNY+V+GI+NLRVVDGSTF DSPGTNPMAT+MMLGRYVGL 
Sbjct: 481 TVTTYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFVDSPGTNPMATVMMLGRYVGLK 540

Query: 541 ILRERSS 547
           I +ERS 
Sbjct: 541 IKQERSK 546

BLAST of CsGy2G005906 vs. ExPASy TrEMBL
Match: A0A5A7UQ82 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G001000 PE=3 SV=1)

HSP 1 Score: 851 bits (2198), Expect = 5.10e-308
Identity = 410/519 (79.00%), Postives = 462/519 (89.02%), Query Frame = 0

Query: 17  SMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLTSN 76
           S L FQ G  LSSNT  NEDF+YM FV DAS+LP  E+YDYI+IGGGTAGCPLA TL+S 
Sbjct: 2   STLHFQFGFPLSSNTNSNEDFKYMNFVQDASELPENEEYDYIVIGGGTAGCPLATTLSST 61

Query: 77  FSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSM 136
           FSVLLLERG+ PTKYP+VL+E++F N +T+EDDGENPFQRFVSEDGVE +RGRVLGG+SM
Sbjct: 62  FSVLLLERGNVPTKYPTVLREETFPNAFTIEDDGENPFQRFVSEDGVEIIRGRVLGGSSM 121

Query: 137 LNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGV 196
           LN GFYSRGHQEFF+ +GV WDMELV+KAYEWVEESVVFEA++NNGWQ AFR  LLEAGV
Sbjct: 122 LNAGFYSRGHQEFFDISGVDWDMELVEKAYEWVEESVVFEANINNGWQNAFRKALLEAGV 181

Query: 197 GPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGLSA 256
           GPYNGF+LNHR+GTKIGGSIFDKEGNRHGS+ELLNKA PNNLKV ++ATV+KI+FS LSA
Sbjct: 182 GPYNGFDLNHRIGTKIGGSIFDKEGNRHGSLELLNKAHPNNLKVAIQATVQKILFSDLSA 241

Query: 257 SGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPH 316
           +GV YSD KG LHTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL+LPVVL QPH
Sbjct: 242 TGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESLELPVVLDQPH 301

Query: 317 VGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPHRF 376
           VGQSMSDNPR+  +++LPY L  +AVK VGTL++N+HLQSITGFLPFSLPPSFSL+P   
Sbjct: 302 VGQSMSDNPRYNVHVILPYQLATSAVKTVGTLENNVHLQSITGFLPFSLPPSFSLVPPGL 361

Query: 377 NSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGD 436
           +SVNLSLA+IVGKFSEV SEGSL L SSTDVKKNP V FNYYSHPDDLAKCVRGVRK G+
Sbjct: 362 DSVNLSLASIVGKFSEVLSEGSLYLTSSTDVKKNPSVSFNYYSHPDDLAKCVRGVRKMGE 421

Query: 437 FLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYWHYHGGCLVGK 496
            LKT  +EKIKIQDFEG + FAF+EP +PENLSD G VE+FCK+TVTTYWHYHGGCLVGK
Sbjct: 422 LLKTEAMEKIKIQDFEGNKRFAFVEPSLPENLSDVGSVEEFCKKTVTTYWHYHGGCLVGK 481

Query: 497 VVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 535
           VVDGNY+V+GI+NLRVVDGSTF DSPGTNPMAT+MMLGR
Sbjct: 482 VVDGNYKVIGIENLRVVDGSTFIDSPGTNPMATVMMLGR 520

BLAST of CsGy2G005906 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 529.3 bits (1362), Expect = 3.8e-150
Identity = 268/547 (48.99%), Postives = 374/547 (68.37%), Query Frame = 0

Query: 9   SFLLFILISMLQFQLGVSLSSNTKP--NEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAG 68
           S LL+  + +L   LGV   SN +P  N    +M+F+ +A+D  +++ YDYII+GGGTAG
Sbjct: 7   SSLLYTALVVL-LLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAG 66

Query: 69  CPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENL 128
           CPLAATL+ +F VLLLERG  P   P+V+    FL   T  ++ ++P Q F+SE+GV N 
Sbjct: 67  CPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNA 126

Query: 129 RGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYA 188
           RGRVLGG+S +N GFYSR  ++FFE +G+ WD+  V ++YEWVE ++VF   L   WQ A
Sbjct: 127 RGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRT-WQTA 186

Query: 189 FRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATV 248
            R+ LLE GV P+NGF L H++GTKIGGS FD+ G RH S +LL  A+ +N++V V ATV
Sbjct: 187 IRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATV 246

Query: 249 EKIIF--------SGLSASGVLYSDFKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVG 308
           E+++         S +SA GV+Y D  GR H A IR +GE+ILSAGA+GSPQLL LSG+G
Sbjct: 247 ERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIG 306

Query: 309 PKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSIT 368
           P+S+LS+  +PV L QPHVG  + DNPR   +IV P P+  + ++VVG  +D   L++ +
Sbjct: 307 PRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAAS 366

Query: 369 GFLPFSLPPSFSLLPHRFNSVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYY 428
             +PF+ P     +    + + + + TI+ K     S G L+L +STDV+ NP+VRFNY+
Sbjct: 367 NVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYF 426

Query: 429 SHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFC 488
           S P DL +CV G RK G+ L++  ++   I+++ G R F F+  P+P + S+D V+  FC
Sbjct: 427 SDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFC 486

Query: 489 KETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVG 546
           + TV+T WHYHGG +VGKVVD + +V+G+ +LR+VDGSTF+ SPGTNP ATLMMLGRY+G
Sbjct: 487 RRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMG 546

BLAST of CsGy2G005906 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 418.3 bits (1074), Expect = 9.4e-117
Identity = 251/581 (43.20%), Postives = 336/581 (57.83%), Query Frame = 0

Query: 9   SFLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAGCP 68
           S+ LF+ +S+  F      SS+  PN       F+ DA+  PT   YDYIIIGGGTAGCP
Sbjct: 7   SWRLFVALSL--FLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCP 66

Query: 69  LAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRG 128
           LAATL+ N SVLLLERG  P   P++ +  +F      +    +P QRFVSEDGV N R 
Sbjct: 67  LAATLSQNASVLLLERGDSPYNNPNITRLSAF-GAALSDLSESSPSQRFVSEDGVINARA 126

Query: 129 RVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYAFR 188
           RVLGG S LN GFY+R   ++    G  WD  L  ++Y+WVE  V F+  +   WQ A R
Sbjct: 127 RVLGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGR-WQTAVR 186

Query: 189 NGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATVEK 248
           +GLLEAG+ P NGF  +H  GTK GG+IFD+ GNRH + +LL  A P  + V++ ATV +
Sbjct: 187 DGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHR 246

Query: 249 IIFSGLS-----ASGVLYSDFKGRLHTASIRK--KGEIILSAGAIGSPQLLLLSGVGPKS 308
           I+F         A+GV+Y D  G+ H A +++    EIILSAG +GSPQLL+LSGVGP +
Sbjct: 247 ILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSA 306

Query: 309 HLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITG-- 368
            L +  + VV+ QPHVGQ M DNP     +  P P+ ++ ++VVG   +  ++++  G  
Sbjct: 307 QLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGEN 366

Query: 369 -----------------FLPFSLPPSFSLLPHRFNSVNLSLAT------IVGKFSEVFSE 428
                            +  FS  P  +LL    +   LS A       ++ K     S 
Sbjct: 367 FGGGGGGSSGSSSTRDYYAMFS--PRATLLESN-SMTKLSSAQPFQGGFLLEKVMGPLST 426

Query: 429 GSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRS 488
           G L+L  + + K NPIV FNY+ HPDDL +CVRG++     +++    + K  D     S
Sbjct: 427 GHLEL-KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----S 486

Query: 489 FAF---LEPPIPENL----SDDGV-----VEKFCKETVTTYWHYHGGCLVGKVVDGNYRV 546
           F +   L    P NL    S  G       E+FC+ TVTT WHYHGGC+VG+VVDG+Y+V
Sbjct: 487 FEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKV 546

BLAST of CsGy2G005906 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 410.6 bits (1054), Expect = 2.0e-114
Identity = 233/530 (43.96%), Postives = 317/530 (59.81%), Query Frame = 0

Query: 55  YDYIIIGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPF 114
           YDYI+IGGGTAGCPLAATL+ NFSVL+LERG  P    +V   ++F ++   +    +  
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNF-HIGLADISASSAS 122

Query: 115 QRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVV 174
           Q FVS DGV N R RVLGG S +N GFYSR    F + AG  WD +LVK++Y WVE  +V
Sbjct: 123 QAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVEREIV 182

Query: 175 FEASLNNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQ 234
            +  L   WQ A R+ LLE GV P+NGF  +H  GTKIGG+IFD+ G RH + ELL  A 
Sbjct: 183 HQPKLTL-WQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYAN 242

Query: 235 PNNLKVVVRATVEKIIF--SGL--SASGVLYSDFKGRLHTA--SIRKKGEIILSAGAIGS 294
           P  L+V++ ATV+KI+F  SG     +GV++ D KG  H A  S RK  E+ILS+GAIGS
Sbjct: 243 PQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGS 302

Query: 295 PQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTL 354
           PQ+L+LSG+GPK  L  LK+PVVL   HVG+ M+DNP  T  +    P+  + ++ VG  
Sbjct: 303 PQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGIT 362

Query: 355 DDNIHLQSITGF--LPFSLPPSFSLLPHR---FNSV-----------------------N 414
              +++++ TGF   P S+   + ++ ++   F+++                        
Sbjct: 363 KMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEA 422

Query: 415 LSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDFLKT 474
            + + I+ K +   S G L L  +T+V  NP V FNY+ HP DL +CV  +R     + +
Sbjct: 423 FNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTS 482

Query: 475 PTIEKIKIQDFEGKRSFAFLEPPI-----PENLSDDGVVEKFCKETVTTYWHYHGGCLVG 534
                    D +       L         P+ L+D   + +FCK+TV T WHYHGGCLVG
Sbjct: 483 NRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVG 542

Query: 535 KVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRER 546
           KVV  N +V+G+  LRV+DGSTF +SPGTNP AT+MM+GRY+G+ ILRER
Sbjct: 543 KVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

BLAST of CsGy2G005906 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 3.1e-112
Identity = 235/552 (42.57%), Postives = 319/552 (57.79%), Query Frame = 0

Query: 39  YMKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLTSNFSVLLLERGSEPTKYPSVLKEQ 98
           Y  F+ DA+  P   ++DYIIIGGGT+GC LAATL+ N SVL+LERG  P   P+    +
Sbjct: 30  YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89

Query: 99  SFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGVKWD 158
           +F    +     ++  Q F+SEDGV N R RVLGG S+LN GFY+R   E+ +    +W 
Sbjct: 90  NFATTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKE--TEWK 149

Query: 159 MELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGVGPYNGFELNHRLGTKIGGSIFD 218
            + V+ AYEWVE+ V F+  +  GWQ AF++GLLEAG  PYNGF  +H  GTKIGG+IFD
Sbjct: 150 TDEVEAAYEWVEKKVAFQPPV-LGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFD 209

Query: 219 KEGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGL-----SASGVLYSDFKGRLHTASI 278
           + G+RH + +LL  A P N+ V + A+V KI+F+        A GV++ D  G LH A +
Sbjct: 210 RAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAEL 269

Query: 279 RKK--GEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL-PVVLHQPHVGQSMSDNPRFTAN 338
            K    E+ILSAGAIGSPQLL+LSG+GP +HL++  + P+VL  P VGQ M DNP     
Sbjct: 270 EKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIF 329

Query: 339 IVLPYPLVITAVKVVGTLDDNIHLQSITGFL-------------------------PFSL 398
           I  P P+ ++ ++VVG      +++  +G +                           + 
Sbjct: 330 IPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTT 389

Query: 399 PPSFSL--LPHRFNSVNLSL------ATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNY 458
            P+ S   +   FN +N  L        I+ K +   S G L+L  +T+   NP VRFNY
Sbjct: 390 SPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFNY 449

Query: 459 YSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQD--FEGKRSFAFLEPP--IPENLSDDGV 518
           Y  P+DL  CV G+      + +    K K  D    G        P    P +++    
Sbjct: 450 YQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFN 509

Query: 519 VEKFCKETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMML 546
           + +FC +TV T WHYHGGC VG+VVD NYRV+GI +LRV+DGSTF  SPGTNP AT+MML
Sbjct: 510 LRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMML 569

BLAST of CsGy2G005906 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 7.0e-112
Identity = 250/574 (43.55%), Postives = 340/574 (59.23%), Query Frame = 0

Query: 10  FLLFILISMLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAGCPL 69
           F  FIL+++  F    S  S+   N  +R+MK   DA+  P    +DYIIIGGGTAGC L
Sbjct: 8   FFQFILVAVFIFH--DSCYSDEAGN--YRFMK---DATLAPKLSHFDYIIIGGGTAGCAL 67

Query: 70  AATLTSNFSVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPF-QRFVSEDGVENLRG 129
           AATL+ N +VL+LERG  P   P+     +F N  T+ +   N + Q F+SEDGV N R 
Sbjct: 68  AATLSQNATVLVLERGGSPYDDPAATDIGNFAN--TLLNITPNSWSQLFISEDGVFNSRA 127

Query: 130 RVLGGTSMLNGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYAFR 189
           RVLGG +++N GFYSR  ++F   AG  W+ + V+ AYEWVE+ VVFE  +N  WQ AFR
Sbjct: 128 RVLGGGTVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNK-WQSAFR 187

Query: 190 NGLLEAGVGPYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATVEK 249
           +GLLEAGV PYNGF   H +GTK GG+IFD++G+RH +  LL  A PN + V + A+V K
Sbjct: 188 DGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHK 247

Query: 250 IIFS-----GLSASGVLYSDFKGRLHTASIRKK----GEIILSAGAIGSPQLLLLSGVGP 309
           I+F+        A GV++ D  G  + A +  +     E+ILSAGAI SPQLL+LSGVGP
Sbjct: 248 ILFTIKGNQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGP 307

Query: 310 KSHLSSLKL-PVVLHQPHVGQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSIT 369
            +HL++ ++ PV++ QP VGQ M DNP     I  P P+ ++ V+ VG      +++  +
Sbjct: 308 AAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGS 367

Query: 370 GF-LPFSLPPSF----------SLLPHR-----FNSVNLSL------ATIVGKFSEVFSE 429
              L  SL  SF          + LP +     F S++L+L        I+ K +   S 
Sbjct: 368 ALSLSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSR 427

Query: 430 GSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDFLKTPTIEKIKIQDFEGKRS 489
           G L+L  +T+   NP V FNY+  P+DL KCV G+      + +    K K       R 
Sbjct: 428 GHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARG 487

Query: 490 FAFLEPPIPENLSDDGV-----VEKFCKETVTTYWHYHGGCLVGKVVDGNYRVMGIKNLR 546
              L   +P NL    +     +E++C +TV T +HYHGGC VGKVVD NY+V+G+  LR
Sbjct: 488 LLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALR 547

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527062.5e-15451.01(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
P527075.5e-15451.10(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
Q945K24.0e-15250.09(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
O827848.2e-15050.63(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
Q9SSM25.3e-14948.99(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_031736342.10.099.82(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical pro... [more]
XP_031736419.10.099.63(R)-mandelonitrile lyase 1-like [Cucumis sativus][more]
XP_008451933.10.093.83PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
KAA0067300.10.094.56(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10911.1 (R)-mande... [more]
XP_038891172.10.083.21(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A1S3BS250.093.83(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=... [more]
A0A5A7VJA10.094.56(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BTD30.079.11(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493053 PE=3 SV=... [more]
A0A0A0LJ830.078.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055560 PE=3 SV=1[more]
A0A5A7UQ825.10e-30879.00(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
AT1G73050.13.8e-15048.99Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.19.4e-11743.20Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.12.0e-11443.96Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.13.1e-11242.57Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.17.0e-11243.55Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 41..342
e-value: 1.6E-57
score: 193.6
coord: 360..547
e-value: 5.4E-32
score: 109.2
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 55..325
e-value: 3.7E-34
score: 118.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 284..298
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 128..151
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 394..535
e-value: 3.1E-29
score: 102.3
NoneNo IPR availableGENE3D3.30.410.40coord: 175..484
e-value: 2.8E-165
score: 552.9
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 7..546
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 7..546
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 376..491
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 55..534
e-value: 2.8E-165
score: 552.9
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 39..545

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G005906.1CsGy2G005906.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0043531 ADP binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0003953 NAD+ nucleosidase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors