CsGy2G005740 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G005740
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description4-coumarate--CoA ligase
LocationGy14Chr2: 4039137 .. 4044567 (+)
RNA-Seq ExpressionCsGy2G005740
SyntenyCsGy2G005740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATTAACATCATACTCATCCATTTGATCATCAACTCCACCTCTAACACCACCTACTCCATAATTACTTCTACAAAAAGCACAACATTTCTCACAAAATTTCAAAAACTTCAAACCAAAATCCCTCATTTTCACATCAAACCAAACCAAAACAACCTTAGAAATCATGGATCATGATGAACAAAGGAACGGCAAAGATTTCATCTTCAAATCAAAGCTCCCAGACATTTACATCCCAAACCACCTCCCTCTCCATACCTATTGCTTTGAACACCTCTCCCAATTCCAACACCGGCCTTGCCTCATCAACGGCACCACCGGCCGCATCCACACCTACGCCGAGGTCAACCTAGCAGCCAGACGTTTCGCTGCGGGGCTGAGCAAGATCGGTGTCGGACAAGGTGACGTGATAATGCTCATGCTCCAGAATAGCCCCGAGTTCGTGTTTGCGTTCCTCGGTGCATCTTTCGCCGGTGCAATTGCCACGACCGCGAACCCGTTGTATAAGCCTGGAGAGATAGTGAAGCAGGCGGTGGCAGCGAAGGTGAAGGTGATAATTACACAAGGAGAGTTTGTGGAGAAGGTGTGGGAATTTGCGGTGGAACATGGGGTGAAGATATTGTGTACGGATTCCTCGTCGGCCTCGGAGTCAGCGGGTTGTTTGAAATTCTCGGAGGTTATGGAGGCCGATGAGAATGAGATTCCGGCGGTGAAGATTAATTCAAATGATGTTGTTGCTCTTCCTTTCTCGTCGGGAACTACCGGAGTTCCAAAAGGAGTTATGCTAACGCATAAATCTCTTGTTACAAGCGTAGCTCAGCAGGTTCTTGCCAAACTTCTGATTATTAACCTTTTTTTTAAATCTCAACCAAAATTTTGAAAAATAATGAAACATATTTCCAAATATAATAAAATATTATAATTTTTTAAAAAAATTATAATAGATAACACATATTTTAGTTTACTTTTTTATATCCAATTAAAAACAACCCTTCAACTTTCATATTTTATAAACTTTTTTTTAATACGATGCATAATTATTTATTTAATATGAAAGTTGTACCATTAATTTTGTTGAAATTGCAACCAAAGTCCAACATCAATTAGATGTAGGAATGATCATATATATTTAAATGTAAACAACTTTCACTCTATTAGTACGAAATCTTTTAATTCGAAACTAAAAGTAAGTTTGTATTTAAAATAGATGATATCATATCATTGTGGAGATATCATATCAAAGTGTTCAAAATGTTTTACCTTTATATATTTATATACTTTCTTTCATTTTATTTTGTGAAGTTTCTATGTTTTTTTTTTTTTCCCTTATTTTCATAAACTTTAGATGAGGAGGAATATTAAATTTCCTTTAAAAACTTTTCTTTTTGAAAAAAAAACCTTTGCAATAAAAAATTAACTTTGTTCAATTTCGTTATATTTTTAAATGATGTTGAGATGGTATGGTAGGAAGATGACTAACATAATATACCATAAGAAATTTTCTCTTAATTATTTAGAGTTGACCACCCTTGATTTTCCTTTTAATAACATGAGATCTAAAACGATAAATAAAAGATAATTGTTTTTTCCTATCAAAAAATGAATGGTAACGTATTTAATATCAACAATCAATAATGAAATAAAGATAGGAGTTAAAGTTTTTTCTCTTTCAAAACGGTAAGTGGGATATGAAAATATAAAGCTCTGATTTAAAAAAACTAAATATATATTAATTATTGTTCAACTGTACATATTTTTTAAGTCATATTTATTGTTGTATATCAACGTTTTTTCATATTTGATCGTTGAAAATTCTCTTTTTATTTTTTTATGTTTGTAGGTGGATGGAGAGAATCCAAATGTAAACATGAATAGGAAGGATGTGATCATATGTGTTCTTCCTTTATTCCATATCTATTCTCTCAACTCCGTTCTCCTGTGTGGACTACGTGTCGGCGCTGCCATTCTCATCCTTCAAAAGTACGACGTCTCCTCTCTTCTCCGTCTCATTCAAACCCACAAAGCCACAATTGCTCCATTTGTCCCTCCCATCGTCTTGGACTTTGCCAAAAACCCTGACATCCATCGTTACGACTTGTCGTCTATTCGAATCGTCATGTCGGGTGCTGCCCCCATGGGGAAGGACCTCGAGGACACCGTCAAAGCTAAGCTCCCCAATGCCATTCTCGGACAGGTAATTCACATTCAATTATATATGCTTTCACTTCTTGTTTTTGTGCAATAAATTTTTTCACTCAACGTAAGTCTAACGACAGTATATCACAAAGTATTATAATTAACTTATAATGTTTGGAGGTGATTTGAAATTATTATTAAAAATACAAGTTTAGAATTACTCTAAAACATACCTTTCCTCTCATGTTTACTTTTCGTAATTATTCTAGTTCATTTTAGTTTAAAAGTATAAAGTAATTTTCAATATCTAATATTAAAGTCAATTTAGATTTATAAATATTTTGAAAAACATTTACAAATATTAAATTAGAATATACAACACAATATATGTTAGTTGGCGACTTGCTGGTTCTGGTAGTGATGAGAATGTTTCGAGTTCTTAAAATATCACCAACCATTTTCAAGTTGTTTTGTATATATTTTAAAAGTGTCTAAATCTTATTTTATTTATAAATTGTTTACTCTATGCTTCTAAATGTTTTATTCATTATTTTAATATGAGAAGTGAGAATTAACAATTATCTTTAGGTTAATTATTGATTTATTGTTAATTGCTAATAGCCTTAGGTTATACAAAATTTATATCCGTCTAATTTTTTTCCTTCTTATTATAAATAATATTTCTACTTTGAAATGTAACATTTTGAATTGATATAATGTTTTACATTTAGTCATAATTTTTTTTCCAATGCTCAAAACATGGTCGAGAGAACAAACTTCTCCCATTGCCAACTTCACTTTATTTAACTGCAGGGAGAAAAACCTTTTTATTTGCAGTGTTATATATATATATTAGCAGAGTAAAATAACTCATTTTTGAAAAATAACAAAAAATATTGACTTTTGAAACAATTGAAAGCTTGATTCTTCTTAAAGCAACAATAATGATTAATGCATTAATGTTTGTGAGGCCATATCATGCTAGGTTTTTAAGAGGAATGATTCAAACGTGACAACTTTAAGCAAGTGGGATATTAATGAATCAATTAATTACTAATAATATTTGTTATTTAATTAAAACAATAGACCGACTTGGATCAAGTCAAATTTTCTCAATAGTTTTTTATTAAAAGAATATTTTTCGTTATTTTATTTTAGTTTCTTTCACTATTTAATTTTCATATTTTAGTAAATCTTAATAATTATATTAATCTAATTTCACATACTATAAAGACTCAAATAACTCTTACAAAAATAAAAAATACTAAACTTATGAATTATTTTTAAATATAACAAAACGAATAAATTTATATTTTTAACAGTTTTATCATTTACCTTGTAAAATTAATTTCTAATGTCTTTTCTATTATATTATAATTTAGTTCACATTTAGTTGGGTATAGTCTACCAAACTTATTGGTTTAAAAATAATTTTTTCATTAAAAATTATTTGAATGTATGTAAGAAGAATATCCCAAGTGTACTCGGAGAATATGACAATATTATGGCCTGACCCTTTTGTTTGTATATGTTTAGAGTAATGCAATGAAGTTTTAAAATAATTTGTATTGTATAGTATAGTATATGACAAATAGTAACAAATGATATTATTACAAAAAAAGTAATGAGATTTATCATAAATAGGGGTATGGAATGACGGAGGCAGGGCCAGTGCTGTCGATGTGTTTGGGATTTGCAAAGGAAGCATTCAAGGTAAAATCAGGTGCATGCGGGACAGTAGTAAGGAATGCTGAGATGAAGATCATACACCCTCAAACTTCTCTTTCTTTGCCTCGTAATAACCCTGGTGAGATCTGCATACGTGGCCCCCAGATCATGAAAGGCAAGTCCCTACCTCCAACATATACACACCCTTTGTTTAACAAAACCATTCATTTACGTGTGATAGATTAAATACACAATAAGGCTTATATATCAAAGATCATAGGAATTTTTTAGTTTTTAGATGAAATTATTTCTTGTTTATCTAATGAGAGTTGAGATGTTATCGATAGGTTACCTTAATAACAAAGAAGCAACCGAGAAGACTATAGACAAAGATGGGTGGCTCCACACAGGAGACTTAGGGTACATTGACGATGACGATGAGATCTTCATTGTCGATCGATTGAAGGAACTTATTAAATACAAAGGATACCAAGTTGCTCCAGCTGAGCTTGAAGCTCTCTTGACTTCAAACCCTAACATTACTTCAGCAGCTGTCATACCGTAAGCATTCCCACGAACATCTTGTACTGCATATCATACATTATTTGTCTTTTACATTATAATAATCTATTTATTTTATTTTTAACAAAATAAATTATTGTGATTCTTCACAGTATGGAAGATGAAGCAGCAGGAGAGATCCCTGTTGCATTTGTTGTTAGATCTAAAGGCTCCAAAATTAGTGAGAATGACATCAAAAACTATATTTCAGATCAGGTTGGTTTATACTTTTATTTTTTTTTCTCTCTCTGTTGTGATATTTATTATTTACGTCATATTTCAATCTACGGATTATATTACAATGAACTCTTACTTGAACATAGCTTTATTGGAATTAAAAAATGAACGTAGTTGTAACTGGTTCAAACATGAAGTATACCTAAATTCAAGAAATTCAAGATTTGAGTTTATACACACATATATTTATACTATAGTCTTCCTTTTTACAAATATTTATAAATTTTTATGGAGCATGGCTTTATATATATAGTCATATAAACATTTCTACTTATTAATGTCATCTATCACAAAGTTGCACTAATGTTGGCTAAGTCCTACCTGTTTGATTGATGTTATATATATACGTGTGTGTATATATATAGATATAAACTCGTTGGTTGAGCTTTTGTGGGACAGTAAGTATTTAATACTGACATGGGTGTAATATTTTTTGGTGGGTGTAGGTTATATATTACAAAAAAATCAGAAAGGTTTTCTTTGTGGACTCAATTCCAATGGCTCCTTCAGGCAAGATATTGAGGAAAATTTTAAAAGCTCAGTTGGAGGCTGGTGTATTTTAATTAAATTATATTAGCTAATTCATCACTCAATAATCGATATGGATCACTTTAACCATGGTCATATATATATGTGTGTGTCTCCATATAGCTTTGAGTTTTTGATCTCTACAAAACAAATGTTATGAAAAGAGAGATCATTACTTCTCAAAAGAAGACGGGTTTATTCATTTGTTATGAATAGTATGTAATCGATCTATATGCCTTTGTAACTTTATTGTATATGTTGATTAAGCTACAATGCAAATGTTTACTAATATATACGTACA

mRNA sequence

ATAATTAACATCATACTCATCCATTTGATCATCAACTCCACCTCTAACACCACCTACTCCATAATTACTTCTACAAAAAGCACAACATTTCTCACAAAATTTCAAAAACTTCAAACCAAAATCCCTCATTTTCACATCAAACCAAACCAAAACAACCTTAGAAATCATGGATCATGATGAACAAAGGAACGGCAAAGATTTCATCTTCAAATCAAAGCTCCCAGACATTTACATCCCAAACCACCTCCCTCTCCATACCTATTGCTTTGAACACCTCTCCCAATTCCAACACCGGCCTTGCCTCATCAACGGCACCACCGGCCGCATCCACACCTACGCCGAGGTCAACCTAGCAGCCAGACGTTTCGCTGCGGGGCTGAGCAAGATCGGTGTCGGACAAGGTGACGTGATAATGCTCATGCTCCAGAATAGCCCCGAGTTCGTGTTTGCGTTCCTCGGTGCATCTTTCGCCGGTGCAATTGCCACGACCGCGAACCCGTTGTATAAGCCTGGAGAGATAGTGAAGCAGGCGGTGGCAGCGAAGGTGAAGGTGATAATTACACAAGGAGAGTTTGTGGAGAAGGTGTGGGAATTTGCGGTGGAACATGGGGTGAAGATATTGTGTACGGATTCCTCGTCGGCCTCGGAGTCAGCGGGTTGTTTGAAATTCTCGGAGGTTATGGAGGCCGATGAGAATGAGATTCCGGCGGTGAAGATTAATTCAAATGATGTTGTTGCTCTTCCTTTCTCGTCGGGAACTACCGGAGTTCCAAAAGGAGTTATGCTAACGCATAAATCTCTTGTTACAAGCGTAGCTCAGCAGGTGGATGGAGAGAATCCAAATGTAAACATGAATAGGAAGGATGTGATCATATGTGTTCTTCCTTTATTCCATATCTATTCTCTCAACTCCGTTCTCCTGTGTGGACTACGTGTCGGCGCTGCCATTCTCATCCTTCAAAAGTACGACGTCTCCTCTCTTCTCCGTCTCATTCAAACCCACAAAGCCACAATTGCTCCATTTGTCCCTCCCATCGTCTTGGACTTTGCCAAAAACCCTGACATCCATCGTTACGACTTGTCGTCTATTCGAATCGTCATGTCGGGTGCTGCCCCCATGGGGAAGGACCTCGAGGACACCGTCAAAGCTAAGCTCCCCAATGCCATTCTCGGACAGGGGTATGGAATGACGGAGGCAGGGCCAGTGCTGTCGATGTGTTTGGGATTTGCAAAGGAAGCATTCAAGGTAAAATCAGGTGCATGCGGGACAGTAGTAAGGAATGCTGAGATGAAGATCATACACCCTCAAACTTCTCTTTCTTTGCCTCGTAATAACCCTGGTGAGATCTGCATACGTGGCCCCCAGATCATGAAAGGTTACCTTAATAACAAAGAAGCAACCGAGAAGACTATAGACAAAGATGGGTGGCTCCACACAGGAGACTTAGGGTACATTGACGATGACGATGAGATCTTCATTGTCGATCGATTGAAGGAACTTATTAAATACAAAGGATACCAAGTTGCTCCAGCTGAGCTTGAAGCTCTCTTGACTTCAAACCCTAACATTACTTCAGCAGCTGTCATACCTATGGAAGATGAAGCAGCAGGAGAGATCCCTGTTGCATTTGTTGTTAGATCTAAAGGCTCCAAAATTAGTGAGAATGACATCAAAAACTATATTTCAGATCAGGTTATATATTACAAAAAAATCAGAAAGGTTTTCTTTGTGGACTCAATTCCAATGGCTCCTTCAGGCAAGATATTGAGGAAAATTTTAAAAGCTCAGTTGGAGGCTGGTGTATTTTAATTAAATTATATTAGCTAATTCATCACTCAATAATCGATATGGATCACTTTAACCATGGTCATATATATATGTGTGTGTCTCCATATAGCTTTGAGTTTTTGATCTCTACAAAACAAATGTTATGAAAAGAGAGATCATTACTTCTCAAAAGAAGACGGGTTTATTCATTTGTTATGAATAGTATGTAATCGATCTATATGCCTTTGTAACTTTATTGTATATGTTGATTAAGCTACAATGCAAATGTTTACTAATATATACGTACA

Coding sequence (CDS)

ATGGATCATGATGAACAAAGGAACGGCAAAGATTTCATCTTCAAATCAAAGCTCCCAGACATTTACATCCCAAACCACCTCCCTCTCCATACCTATTGCTTTGAACACCTCTCCCAATTCCAACACCGGCCTTGCCTCATCAACGGCACCACCGGCCGCATCCACACCTACGCCGAGGTCAACCTAGCAGCCAGACGTTTCGCTGCGGGGCTGAGCAAGATCGGTGTCGGACAAGGTGACGTGATAATGCTCATGCTCCAGAATAGCCCCGAGTTCGTGTTTGCGTTCCTCGGTGCATCTTTCGCCGGTGCAATTGCCACGACCGCGAACCCGTTGTATAAGCCTGGAGAGATAGTGAAGCAGGCGGTGGCAGCGAAGGTGAAGGTGATAATTACACAAGGAGAGTTTGTGGAGAAGGTGTGGGAATTTGCGGTGGAACATGGGGTGAAGATATTGTGTACGGATTCCTCGTCGGCCTCGGAGTCAGCGGGTTGTTTGAAATTCTCGGAGGTTATGGAGGCCGATGAGAATGAGATTCCGGCGGTGAAGATTAATTCAAATGATGTTGTTGCTCTTCCTTTCTCGTCGGGAACTACCGGAGTTCCAAAAGGAGTTATGCTAACGCATAAATCTCTTGTTACAAGCGTAGCTCAGCAGGTGGATGGAGAGAATCCAAATGTAAACATGAATAGGAAGGATGTGATCATATGTGTTCTTCCTTTATTCCATATCTATTCTCTCAACTCCGTTCTCCTGTGTGGACTACGTGTCGGCGCTGCCATTCTCATCCTTCAAAAGTACGACGTCTCCTCTCTTCTCCGTCTCATTCAAACCCACAAAGCCACAATTGCTCCATTTGTCCCTCCCATCGTCTTGGACTTTGCCAAAAACCCTGACATCCATCGTTACGACTTGTCGTCTATTCGAATCGTCATGTCGGGTGCTGCCCCCATGGGGAAGGACCTCGAGGACACCGTCAAAGCTAAGCTCCCCAATGCCATTCTCGGACAGGGGTATGGAATGACGGAGGCAGGGCCAGTGCTGTCGATGTGTTTGGGATTTGCAAAGGAAGCATTCAAGGTAAAATCAGGTGCATGCGGGACAGTAGTAAGGAATGCTGAGATGAAGATCATACACCCTCAAACTTCTCTTTCTTTGCCTCGTAATAACCCTGGTGAGATCTGCATACGTGGCCCCCAGATCATGAAAGGTTACCTTAATAACAAAGAAGCAACCGAGAAGACTATAGACAAAGATGGGTGGCTCCACACAGGAGACTTAGGGTACATTGACGATGACGATGAGATCTTCATTGTCGATCGATTGAAGGAACTTATTAAATACAAAGGATACCAAGTTGCTCCAGCTGAGCTTGAAGCTCTCTTGACTTCAAACCCTAACATTACTTCAGCAGCTGTCATACCTATGGAAGATGAAGCAGCAGGAGAGATCCCTGTTGCATTTGTTGTTAGATCTAAAGGCTCCAAAATTAGTGAGAATGACATCAAAAACTATATTTCAGATCAGGTTATATATTACAAAAAAATCAGAAAGGTTTTCTTTGTGGACTCAATTCCAATGGCTCCTTCAGGCAAGATATTGAGGAAAATTTTAAAAGCTCAGTTGGAGGCTGGTGTATTTTAA

Protein sequence

MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEAGVF*
Homology
BLAST of CsGy2G005740 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 1.9e-226
Identity = 387/542 (71.40%), Postives = 457/542 (84.32%), Query Frame = 0

Query: 2   DHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVN 61
           DH E     +FIF+SKLPDIYIPNHLPLH+YCFE++SQF+ RPCLING TG I TYA+V+
Sbjct: 8   DHQE-----EFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVD 67

Query: 62  LAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 121
           L +R+ AAGL K+G+ QGDVIML+LQNSPEFV+AFL AS+ GAI TTANP Y P E+ KQ
Sbjct: 68  LTSRKVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQ 127

Query: 122 AVAAKVKVIITQGEFVEKVWEF-AVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 181
           A A+K K++IT   +++KV EF   E GVK++C D+        CL FSE+ +ADE EIP
Sbjct: 128 AAASKTKLVITLAGYIDKVKEFTGGESGVKVMCVDAPPPESE--CLHFSELTQADETEIP 187

Query: 182 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 241
           AVKI+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDG+NPN+  ++ DVI+CVLP
Sbjct: 188 AVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLP 247

Query: 242 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 301
           LFHIYSLNS+LLCGLRVGAAILI+QK+++S LL LI+  K TIAPFVPPIVL  AK PD+
Sbjct: 248 LFHIYSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDL 307

Query: 302 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 361
           HRYDLSSIR VMSG APMGK+LED VK KLP+A LGQGYGMTEAGPVLSMCL FAKE F 
Sbjct: 308 HRYDLSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFP 367

Query: 362 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 421
           +KSGACGTVVRNAEMKI+ P T  SLPRN  GEICIRG QIMKGY+N+ EAT+ TID+ G
Sbjct: 368 IKSGACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGG 427

Query: 422 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 481
           WLHTGD+G+ID+DDE+FIVDRLKELIKYKG+QVAPAELE++L S+PNIT AAV+PM+DEA
Sbjct: 428 WLHTGDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEA 487

Query: 482 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 541
           AGE+PVAFVVRS GSKI+E DIK YIS QV++YK+I K FF++ IP  PSGKILRKIL+A
Sbjct: 488 AGEVPVAFVVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRA 542

Query: 542 QL 543
           +L
Sbjct: 548 KL 542

BLAST of CsGy2G005740 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.3e-221
Identity = 370/542 (68.27%), Postives = 457/542 (84.32%), Query Frame = 0

Query: 5   EQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAA 64
           E +   D IF+SKLPDIYIP HLPLH+YCFE++S+F  RPCLING   +I+TYAEV L  
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 65  RRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVA 124
           R+ A GL+K+G+ Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VKQA A
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 125 AKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKI 184
           +  K+IITQ  FV KV ++A E+ VK++C DS+      GCL FSE+ ++DE+EIP VKI
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSA----PEGCLHFSELTQSDEHEIPEVKI 187

Query: 185 NSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHI 244
             +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+ +DV++CVLPLFHI
Sbjct: 188 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 247

Query: 245 YSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYD 304
           YSLNS+LLCGLRVGAAILI+QK+D++  L LIQ +K +I PFVPPIVL  AK+P +  YD
Sbjct: 248 YSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYD 307

Query: 305 LSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSG 364
           LSS+R VMSGAAP+GK+LED V+ K PNA LGQGYGMTEAGPVL+MCL FAKE F +KSG
Sbjct: 308 LSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 367

Query: 365 ACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHT 424
           ACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TIDK+GWLHT
Sbjct: 368 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHT 427

Query: 425 GDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEI 484
           GD+G+ID+DDE+FIVDRLKELIKYKG+QVAPAE+EALL ++PNI+ AAV+PM+DE AGE+
Sbjct: 428 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEV 487

Query: 485 PVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEA 544
           PVAFVVRS GS I+E+++K++IS QVI+YK++++VFFV+++P +PSGKILRK L+A+L A
Sbjct: 488 PVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAA 545

Query: 545 GV 547
           GV
Sbjct: 548 GV 545

BLAST of CsGy2G005740 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 4.0e-221
Identity = 371/546 (67.95%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MD + +++G D IF+SKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G   RI+TYAEV
Sbjct: 3   MDIETKQSG-DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 62

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ A GL+K+G+ Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 63  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 122

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+  K++ITQ  F  KV ++A+E+ +K++C DS+      GC+ FSE++++DE+EIP
Sbjct: 123 QAKASSAKIVITQACFAGKVKDYAIENDLKVICVDSA----PEGCVHFSELIQSDEHEIP 182

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+  DV++CVLP
Sbjct: 183 DVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLP 242

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +
Sbjct: 243 LFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLV 302

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H YDLSS+R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 303 HNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 362

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+G
Sbjct: 363 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 422

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE 
Sbjct: 423 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 482

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K++IS QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 483 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 542

Query: 541 QLEAGV 547
           +L AG+
Sbjct: 543 RLAAGI 543

BLAST of CsGy2G005740 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 2.6e-220
Identity = 369/546 (67.58%), Postives = 457/546 (83.70%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M  + + N  D IF+SKLPDIYIP HLPLH+YCFE++S+F  RPCLING    I+TYA+V
Sbjct: 1   MPMETETNQGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ AAGL+K+G+ Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 61  ELTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+  K+IITQ  FV KV ++A ++ + ++C DS+      GC+ FSE+ +ADE++IP
Sbjct: 121 QAKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSA----PEGCIHFSELTQADEHDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI S+DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+ +DV++CVLP
Sbjct: 181 DVKIQSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILI+QK+D+     LI+ +K TI PFVPPIVL  AK+P +
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVV 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
             YDLSS+R VMSGAAP+GK+LED V+ K PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 301 DNYDLSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+  AT +TIDK+G
Sbjct: 361 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+GYID+DDE+FIVDRLKELIKYKG+QVAPAELEALL ++PNI+ AAV+PM+DE 
Sbjct: 421 WLHTGDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQ 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K+++S QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 481 AGEVPVAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 540

Query: 541 QLEAGV 547
           +L AGV
Sbjct: 541 RLAAGV 542

BLAST of CsGy2G005740 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 7.6e-220
Identity = 370/546 (67.77%), Postives = 459/546 (84.07%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MD + +++G D IF+SKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G   RI+TYAEV
Sbjct: 3   MDTETKQSG-DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 62

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ A GL+K+G+ Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 63  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 122

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+  K++ITQ  F  KV ++A+E+ +K++C D    S   GC+ FSE++++DE+EIP
Sbjct: 123 QAKASSAKIVITQACFAGKVKDYAIENDLKVICVD----SVPEGCVHFSELIQSDEHEIP 182

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+  DV++CVLP
Sbjct: 183 DVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLP 242

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +
Sbjct: 243 LFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLV 302

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
             YDLSS+R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 303 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 362

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+G
Sbjct: 363 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 422

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE 
Sbjct: 423 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 482

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K++IS QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 483 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 542

Query: 541 QLEAGV 547
           +L AG+
Sbjct: 543 RLAAGI 543

BLAST of CsGy2G005740 vs. NCBI nr
Match: XP_004149075.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN61091.1 hypothetical protein Csa_021253 [Cucumis sativus])

HSP 1 Score: 1081 bits (2795), Expect = 0.0
Identity = 546/547 (99.82%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of CsGy2G005740 vs. NCBI nr
Match: XP_008452293.1 (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1041 bits (2691), Expect = 0.0
Identity = 523/547 (95.61%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 546

BLAST of CsGy2G005740 vs. NCBI nr
Match: KAA0048648.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1019 bits (2634), Expect = 0.0
Identity = 516/547 (94.33%), Postives = 533/547 (97.44%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIR       YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR-------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 539

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 539

BLAST of CsGy2G005740 vs. NCBI nr
Match: TYK10849.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1006 bits (2602), Expect = 0.0
Identity = 511/547 (93.42%), Postives = 528/547 (96.53%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PG            YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPG------------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 534

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 534

BLAST of CsGy2G005740 vs. NCBI nr
Match: XP_038890953.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 488/547 (89.21%), Postives = 516/547 (94.33%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH    NG+DFIF+SKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLING TG +HTYA V
Sbjct: 1   MDHGLM-NGQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGATGEVHTYAAV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARR AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAI+TTANPLYKPGEI K
Sbjct: 61  DLAARRVAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAISTTANPLYKPGEIAK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA  A+ KVIITQ EFVEKVW +AVEHGVKILCTDS       GCL+FSEVMEADEN+IP
Sbjct: 121 QAAGARPKVIITQAEFVEKVWGYAVEHGVKILCTDSPPV----GCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVN+   DVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNLYSNDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILIL+KYD+SSLL LIQT+KATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILKKYDMSSLLPLIQTYKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKA+LPNA LGQGYGMTEAGPVLSMCL FAKE F+
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKARLPNATLGQGYGMTEAGPVLSMCLAFAKEPFE 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKII+P+TS+SLPRN+PGEICIRG QIMKGY+NN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIINPETSVSLPRNHPGEICIRGSQIMKGYINNREATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDD+DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNI+SAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDNDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNISSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISE+D+K YISDQVIYYKKIRKVFFVDSIP+APSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISEDDLKKYISDQVIYYKKIRKVFFVDSIPIAPSGKILRKVLKA 540

Query: 541 QLEAGVF 547
           QLEAG F
Sbjct: 541 QLEAGAF 542

BLAST of CsGy2G005740 vs. ExPASy TrEMBL
Match: A0A0A0LM95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G048330 PE=4 SV=1)

HSP 1 Score: 1081 bits (2795), Expect = 0.0
Identity = 546/547 (99.82%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of CsGy2G005740 vs. ExPASy TrEMBL
Match: A0A1S3BSW8 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 1041 bits (2691), Expect = 0.0
Identity = 523/547 (95.61%), Postives = 540/547 (98.72%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 546

BLAST of CsGy2G005740 vs. ExPASy TrEMBL
Match: A0A5A7U0C7 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1468G00210 PE=4 SV=1)

HSP 1 Score: 1019 bits (2634), Expect = 0.0
Identity = 516/547 (94.33%), Postives = 533/547 (97.44%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIR       YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIR-------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 539

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 539

BLAST of CsGy2G005740 vs. ExPASy TrEMBL
Match: A0A5D3CKT4 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00190 PE=4 SV=1)

HSP 1 Score: 1006 bits (2602), Expect = 0.0
Identity = 511/547 (93.42%), Postives = 528/547 (96.53%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQRNG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PG            YLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPG------------YLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 534

Query: 541 QLEAGVF 547
           QLEAGVF
Sbjct: 541 QLEAGVF 534

BLAST of CsGy2G005740 vs. ExPASy TrEMBL
Match: A0A6J1EP35 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=4 SV=1)

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 475/540 (87.96%), Postives = 507/540 (93.89%), Query Frame = 0

Query: 8   NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRF 67
           N +DFIF+SKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG+IHTYA V+LAARR 
Sbjct: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66

Query: 68  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKV 127
           A GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQA  A+ 
Sbjct: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126

Query: 128 KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSN 187
           KVIITQ EFVEKV EFAVE+GVKI+CTDS       GCL+FSE++EADENEIPAVKINSN
Sbjct: 127 KVIITQAEFVEKVREFAVENGVKIICTDSPPV----GCLRFSEILEADENEIPAVKINSN 186

Query: 188 DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSL 247
           DVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++N  DV+IC+LPLFHIYSL
Sbjct: 187 DVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSL 246

Query: 248 NSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 307
           NSVLLCGLRVGAAILILQKYD+SSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS
Sbjct: 247 NSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 306

Query: 308 IRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACG 367
           IRIVMSGAAPMGK LEDTVKA+LPNA LGQGYGMTEAGPVLSMCLGFAKE F+ KSGACG
Sbjct: 307 IRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACG 366

Query: 368 TVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDL 427
           TVVRNAEMKIIHP+T +SLPRN+PGEICIRG QIMKGYLNNKEATEKTIDK+GWLHTGDL
Sbjct: 367 TVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTGDL 426

Query: 428 GYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVA 487
           G+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAAGEIP A
Sbjct: 427 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAA 486

Query: 488 FVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEAGVF 547
           FVVRSKGSKISE+DIK YISDQVIYYKKIRKVFFVDSIP+APSGKILRK+LKAQLE G F
Sbjct: 487 FVVRSKGSKISEDDIKKYISDQVIYYKKIRKVFFVDSIPVAPSGKILRKVLKAQLENGAF 542

BLAST of CsGy2G005740 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 714.5 bits (1843), Expect = 6.4e-206
Identity = 352/550 (64.00%), Postives = 437/550 (79.45%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M+     N  D IF+SKLPDIYIPNHL LH Y F+++S+F  +PCLING TG ++TY++V
Sbjct: 12  MEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDV 71

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           ++ +R+ AA   K+GV Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI K
Sbjct: 72  HVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAK 131

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILC-TDSSSASESAGCLKFSEVMEAD---E 180
           QA A+  K+IIT+  +V+K+     + GV I+C  D+ S     GCL+F+E+ ++     
Sbjct: 132 QAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEAS 191

Query: 181 NEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVII 240
             I +V+I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +  DVI+
Sbjct: 192 EVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVIL 251

Query: 241 CVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAK 300
           CVLP+FHIY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK
Sbjct: 252 CVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAK 311

Query: 301 NPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAK 360
           + +  +YDLSSIR+V SGAAP+GK+LED V AK PNA LGQGYGMTEAGPVL+M LGFAK
Sbjct: 312 SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 371

Query: 361 EAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTI 420
           E F VKSGACGTVVRNAEMKI+ P T  SL RN PGEICIRG QIMKGYLNN  AT +TI
Sbjct: 372 EPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETI 431

Query: 421 DKDGWLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPM 480
           DKDGWLHTGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M
Sbjct: 432 DKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAM 491

Query: 481 EDEAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRK 540
           ++EAAGE+PVAFVV+SK S++SE+D+K ++S QV++YK+I KVFF +SIP APSGKILRK
Sbjct: 492 KEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRK 551

Query: 541 ILKAQLEAGV 547
            L+A+L  G+
Sbjct: 552 DLRAKLANGL 561

BLAST of CsGy2G005740 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 711.4 bits (1835), Expect = 5.4e-205
Identity = 352/546 (64.47%), Postives = 437/546 (80.04%), Query Frame = 0

Query: 3   HDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNL 62
           +D+++   D IF+S+LPDIYIPNHLPLH Y FE++S+F  +PCLING TG ++TYA+V++
Sbjct: 12  NDQKQCSNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHV 71

Query: 63  AARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQA 122
            +R+ AAGL  +GV Q DV+M++L NSPE V  FL ASF GAI T+ANP + P EI KQA
Sbjct: 72  TSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQA 131

Query: 123 VAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAV 182
            A+  K+I+TQ  +V+K+       GV I+ TDS +  E+  CL+FSE+ +++E  + ++
Sbjct: 132 KASAAKLIVTQSRYVDKIKNLQ-NDGVLIVTTDSDAIPEN--CLRFSELTQSEEPRVDSI 191

Query: 183 --KINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 242
             KI+  DVVALPFSSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  NR DVI+CVLP
Sbjct: 192 PEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLP 251

Query: 243 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 302
           +FHIY+LNS++LC LRVGA ILI+ K++++ LL  IQ  K T+A  VPPIVL  AK+P+ 
Sbjct: 252 MFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPET 311

Query: 303 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 362
            +YDLSS+R+V SGAAP+GK+LED + AK PNA LGQGYGMTEAGPVL+M LGFAKE F 
Sbjct: 312 EKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFP 371

Query: 363 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 422
           VKSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+  AT  TIDKDG
Sbjct: 372 VKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDG 431

Query: 423 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 482
           WLHTGD+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P I   AV+ M++E 
Sbjct: 432 WLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEED 491

Query: 483 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 542
           AGE+PVAFVVRSK S ISE++IK ++S QV++YK+I KVFF DSIP APSGKILRK L+A
Sbjct: 492 AGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRA 551

Query: 543 QLEAGV 547
           +L  G+
Sbjct: 552 RLANGL 554

BLAST of CsGy2G005740 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 666.8 bits (1719), Expect = 1.5e-191
Identity = 329/514 (64.01%), Postives = 407/514 (79.18%), Query Frame = 0

Query: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M+     N  D IF+SKLPDIYIPNHL LH Y F+++S+F  +PCLING TG ++TY++V
Sbjct: 12  MEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDV 71

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           ++ +R+ AA   K+GV Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI K
Sbjct: 72  HVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAK 131

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILC-TDSSSASESAGCLKFSEVMEAD---E 180
           QA A+  K+IIT+  +V+K+     + GV I+C  D+ S     GCL+F+E+ ++     
Sbjct: 132 QAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEAS 191

Query: 181 NEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVII 240
             I +V+I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +  DVI+
Sbjct: 192 EVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVIL 251

Query: 241 CVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAK 300
           CVLP+FHIY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK
Sbjct: 252 CVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAK 311

Query: 301 NPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAK 360
           + +  +YDLSSIR+V SGAAP+GK+LED V AK PNA LGQGYGMTEAGPVL+M LGFAK
Sbjct: 312 SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 371

Query: 361 EAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTI 420
           E F VKSGACGTVVRNAEMKI+ P T  SL RN PGEICIRG QIMKGYLNN  AT +TI
Sbjct: 372 EPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETI 431

Query: 421 DKDGWLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPM 480
           DKDGWLHTGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M
Sbjct: 432 DKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAM 491

Query: 481 EDEAAGEIPVAFVVRSKGSKISENDIKNYISDQV 511
           ++EAAGE+PVAFVV+SK S++SE+D+K ++S QV
Sbjct: 492 KEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of CsGy2G005740 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 663.3 bits (1710), Expect = 1.7e-190
Identity = 325/532 (61.09%), Postives = 415/532 (78.01%), Query Frame = 0

Query: 13  IFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRFAAGLS 72
           IF+SKLPDI IPNHLPLHTYCFE LS    +PCLI G+TG+ +TY E +L  RR A+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 73  KIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKVKVIIT 132
           K+G+ +GDVIM++LQNS EFVF+F+GAS  GA++TTANP Y   E+ KQ  ++  K+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 133 QGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENE--IPAVKINSNDVV 192
             ++V+K+        + ++ TD  +      CL FS ++  DE       V I  +D  
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPE---NCLPFSTLITDDETNPFQETVDIGGDDAA 213

Query: 193 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSLNSV 252
           ALPFSSGTTG+PKGV+LTHKSL+TSVAQQVDG+NPN+ +   DVI+CVLPLFHIYSLNSV
Sbjct: 214 ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSV 273

Query: 253 LLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 312
           LL  LR GA +L++ K+++ +LL LIQ H+ TIA  VPP+V+  AKNP ++ YDLSS+R 
Sbjct: 274 LLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF 333

Query: 313 VMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACGTVV 372
           V+SGAAP+GK+L+D+++ +LP AILGQGYGMTEAGPVLSM LGFAKE    KSG+CGTVV
Sbjct: 334 VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVV 393

Query: 373 RNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDLGYI 432
           RNAE+K++H +T LSL  N PGEICIRG QIMK YLN+ EAT  TID++GWLHTGD+GY+
Sbjct: 394 RNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYV 453

Query: 433 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVV 492
           D+DDEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +I  AAV+P  DE AGE+PVAFVV
Sbjct: 454 DEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVV 513

Query: 493 RSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQL 543
           RS G+ I+E D+K Y++ QV++YK++ KVFFV SIP +PSGKILRK LKA+L
Sbjct: 514 RSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CsGy2G005740 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 645.6 bits (1664), Expect = 3.6e-185
Identity = 314/551 (56.99%), Postives = 419/551 (76.04%), Query Frame = 0

Query: 2   DHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLS----QFQHRPCLINGTTGRIHTY 61
           + +E+    DFIF+SKLPDI+IPNHLPL  Y F+  S          C+I+G TGRI TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 62  AEVNLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGE 121
           A+V    RR AAG+ ++G+  GDV+ML+L NSPEF  +FL  ++ GA++TTANP Y   E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 122 IVKQAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSS-----ASESAGCLKFSEVM 181
           I KQA A+  K+IIT+   V+K+       GV I+C D        +S   GC+ F+E+ 
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLK-NDGVLIVCLDDDGDNGVVSSSDDGCVSFTELT 196

Query: 182 EADENEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRK 241
           +ADE E+   KI+  D VA+P+SSGTTG+PKGVM+THK LVTS+AQ+VDGENPN+N    
Sbjct: 197 QADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAN 256

Query: 242 DVIICVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVL 301
           DVI+C LP+FHIY+L++++L  +R GAA+LI+ +++++ ++ LIQ +K T+ P  PP+VL
Sbjct: 257 DVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVL 316

Query: 302 DFAKNPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCL 361
            F K+P+  RYDLSS+RI++SGAA + K+LED V+ K PNAI GQGYGMTE+G V +  L
Sbjct: 317 AFIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSL 376

Query: 362 GFAKEAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEAT 421
            FAK  FK KSGACGTV+RNAEMK++  +T +SLPRN  GEIC+RG Q+MKGYLN+ EAT
Sbjct: 377 AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEAT 436

Query: 422 EKTIDKDGWLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAA 481
            +TIDKDGWLHTGD+G++DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P+I  AA
Sbjct: 437 ARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAA 496

Query: 482 VIPMEDEAAGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGK 541
           V+ M+DE A E+PVAFV RS+GS+++E+D+K+Y++ QV++YK+I+ VFF++ IP A SGK
Sbjct: 497 VVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGK 556

Query: 542 ILRKILKAQLE 544
           ILRK L+A+LE
Sbjct: 557 ILRKDLRAKLE 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
M4ISH01.9e-22671.404-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1[more]
O241452.3e-22168.274-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
P316854.0e-22167.954-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
I3PB372.6e-22067.584-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
P316847.6e-22067.774-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004149075.10.099.824-coumarate--CoA ligase 1 [Cucumis sativus] >KGN61091.1 hypothetical protein Csa... [more]
XP_008452293.10.095.61PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
KAA0048648.10.094.334-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa][more]
TYK10849.10.093.424-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa][more]
XP_038890953.10.089.214-coumarate--CoA ligase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0LM950.099.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G048330 PE=4 SV=1[more]
A0A1S3BSW80.095.614-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1[more]
A0A5A7U0C70.094.334-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A5D3CKT40.093.424-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1EP350.087.964-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436262 PE=... [more]
Match NameE-valueIdentityDescription
AT1G51680.16.4e-20664.004-coumarate:CoA ligase 1 [more]
AT3G21240.15.4e-20564.474-coumarate:CoA ligase 2 [more]
AT1G51680.31.5e-19164.014-coumarate:CoA ligase 1 [more]
AT1G65060.11.7e-19061.094-coumarate:CoA ligase 3 [more]
AT3G21230.13.6e-18556.994-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 457..532
e-value: 6.0E-13
score: 49.6
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 4..442
e-value: 8.4E-164
score: 547.3
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 36..448
e-value: 3.8E-107
score: 358.5
NoneNo IPR availableGENE3D3.30.300.30coord: 443..545
e-value: 2.0E-29
score: 104.2
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 8..543
NoneNo IPR availablePANTHERPTHR24096:SF3794-COUMARATE--COA LIGASE 2-LIKEcoord: 8..543
NoneNo IPR availableCDDcd059044CLcoord: 23..538
e-value: 0.0
score: 756.382
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 17..544
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 192..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G005740.2CsGy2G005740.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009698 phenylpropanoid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0016491 oxidoreductase activity