CsGy2G004910 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G004910
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr2: 3400734 .. 3402279 (-)
RNA-Seq ExpressionCsGy2G004910
SyntenyCsGy2G004910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGTACGACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGATTCAATCCGTTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAGTAATAATACTAATACAGTACACATTATGAAATTTGGTATTGAGGTTTTGTTCTAATATTGCTGGCAAACCTTTTGCAGAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGGTGCTGTGGCTTCTAAAAACTGATTTTATATTCATAGCATTATTTGAGATTTTGAAGCTATGTAAGGTGCAAGTTAAGTTTGTAGTTAATGATATATATCGTACGGCGTACGCACCTGAGATAGGTTTGAGCAGGATTCTATAGCTCAATCATTGATCAATGTCTGTCTTGTTACGGAATTAATTGTTGCTTTTGTATGGTAAATCAACATGCTTGATTATGTGTTATTGTGATGCACACAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGTACTCAGTTCAAATGTCAATATTCTGTTATTTACCCCATTGAAAGAGACTTACTTTTTAGCAACTTGGGACCATAAATGCATGTTCTTTTGACAATGTAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGATACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTATTTAAAGAGTGCACCATAAAGGGTAGTGGGAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGTAAGTTCAGGGCCAGAGTTCTTTCAAGCTGCACAGTGAAGCATAATAGTCATTTAGGACTTATTGAATTGCCAAGTTCTAACTGGTGTTATATTCTTTAGGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

mRNA sequence

ATGCAGGTACGACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGATTCAATCCGTTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGATACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTATTTAAAGAGTGCACCATAAAGGGTAGTGGGAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

Coding sequence (CDS)

ATGCAGGTACGACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGATTCAATCCGTTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGATACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTATTTAAAGAGTGCACCATAAAGGGTAGTGGGAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

Protein sequence

MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL*
Homology
BLAST of CsGy2G004910 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-81
Identity = 155/330 (46.97%), Postives = 211/330 (63.94%), Query Frame = 0

Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           +++ TLF   V+G+ A  +   + +T      VD +G GNF TVQ+AIDS+ P N++WI+
Sbjct: 14  MVVMTLF---VYGYSAAAEQIAYTIT------VDLNGGGNFTTVQSAIDSISPPNHNWIR 73

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           +    G+Y+EKVTIP EK FIYL+G     TVI +DDHQ TD SATFT+   +I++ GIT
Sbjct: 74  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 133

Query: 128 FENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSH 187
           F+N++N+   P     ++   I  A+AAR+ GD+    +  F G QDTL+D +GRH++  
Sbjct: 134 FKNTYNI--VP-----NNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKR 193

Query: 188 CYIEGAIDFIFGSGQSVYEDCMINVNVA-SLPQVYQGYITAQSRQSAADPSGFVFKECTI 247
           C I G IDFIFG GQS++++C +N+ +    P    G ITA  R S +D  GFVF +CT+
Sbjct: 194 CIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTV 253

Query: 248 KGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAST 307
            G GK LLGRA+G  +RVIF  + +  VV P GW AW  KG E +  +VE  CTG GA T
Sbjct: 254 TGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADT 313

Query: 308 SMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           S RVPW K L  S + GF+  SFI+QDGWI
Sbjct: 314 SQRVPWLKKLSLSEVDGFASVSFIDQDGWI 327

BLAST of CsGy2G004910 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.5e-80
Identity = 145/299 (48.49%), Postives = 192/299 (64.21%), Query Frame = 0

Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           VIVD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+TIP +KPFI + GA   +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARI 157
           T + +DDH     S TF +   N +V+ ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 158 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 217
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 218 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 277
                GYITAQ R +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 278 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            GW+ W+++G E+   Y E  C G G++TS R  W K L AS +   +  SFIN+ GW+
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWV 323

BLAST of CsGy2G004910 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.1e-79
Identity = 143/302 (47.35%), Postives = 188/302 (62.25%), Query Frame = 0

Query: 35  QSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGAD 94
           Q  V VD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 95  SSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIA 154
              T + +DDH     S TF++   N +V+ ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 155 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNV 214
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 215 ASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSV 274
             L     GYITAQ R +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 275 VAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDG 334
           V PEGW AW+F G E   ++ E  C G GA+   RV W K L  S +   +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 328

Query: 335 WI 337
           W+
Sbjct: 331 WV 328

BLAST of CsGy2G004910 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.8e-76
Identity = 142/305 (46.56%), Postives = 191/305 (62.62%), Query Frame = 0

Query: 34  IQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGA 93
           I   +IV+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 94  DSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAI 153
               T+I + DHQ T+TSATFTS P NII+ GITF+N +N+            +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNI--------ASSSSPTKPAV 156

Query: 154 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVN 213
           AA + GDK A  +  F G+QDTL+D  GRH++  C I G IDFIFG  QS++E C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 214 VASLP--QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIM 273
           V   P  +VY G ITAQ R S  D  GFVFK+CT+ GSGKALLGRA+  +SRVIF  ++ 
Sbjct: 217 VGIYPPNEVY-GTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 276

Query: 274 GSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFIN 333
              + P GW AW  KG+E +  +VE  CTG GA TS RVPW        +  F+  +FI+
Sbjct: 277 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 332

Query: 334 QDGWI 337
           ++GW+
Sbjct: 337 EEGWL 332

BLAST of CsGy2G004910 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 128/245 (52.24%), Postives = 161/245 (65.71%), Query Frame = 0

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTY-ITQAIAAR 157
           T IT++ H  TD S+TFTS P +I+VR ++  N++N      L   +  ++ I  A+A  
Sbjct: 48  TTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIKPAVAIS 107

Query: 158 IYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVAS 217
           +YGDKSAF+NC F G QDT+WD  GRH F +CYIEGAIDFIFGSGQSVYEDC IN    +
Sbjct: 108 VYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGA 167

Query: 218 L-PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVV 277
           L  +V  GYITAQ R S +DPSGFVF   ++ GS    LGRAYGPFSRVIF    + SVV
Sbjct: 168 LASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVV 227

Query: 278 APEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHL-TGFSVESFINQDG 337
            PEGWY+WH+ G E +F Y E  C G G+  S RVPW   L + +    FS+ +FI+QD 
Sbjct: 228 HPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQ 287

Query: 338 WIPTV 340
           WI  +
Sbjct: 288 WISNI 290

BLAST of CsGy2G004910 vs. NCBI nr
Match: KGN61008.1 (hypothetical protein Csa_021278 [Cucumis sativus])

HSP 1 Score: 699 bits (1803), Expect = 4.78e-254
Identity = 339/340 (99.71%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQV+PPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV
Sbjct: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT
Sbjct: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340
           GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340

BLAST of CsGy2G004910 vs. NCBI nr
Match: XP_031736683.1 (probable pectinesterase 66 [Cucumis sativus])

HSP 1 Score: 686 bits (1770), Expect = 1.17e-248
Identity = 339/362 (93.65%), Postives = 340/362 (93.92%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQV+PPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFE----------------------NSFNLREAPELFSCDDGTYITQAIAARIY 180
           IIVRGITFE                      NSFNLREAPELFSCDDGTYITQAIAARIY
Sbjct: 121 IIVRGITFEVLWLLKTDFIFIALFEILKLCKNSFNLREAPELFSCDDGTYITQAIAARIY 180

Query: 181 GDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP 240
           GDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP
Sbjct: 181 GDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP 240

Query: 241 QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPE 300
           QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPE
Sbjct: 241 QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPE 300

Query: 301 GWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPT 340
           GWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPT
Sbjct: 301 GWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPT 360

BLAST of CsGy2G004910 vs. NCBI nr
Match: KAA0048077.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 624 bits (1610), Expect = 1.25e-224
Identity = 302/337 (89.61%), Postives = 316/337 (93.77%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ++PPFLLLFTLFNAVVFGFEAVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK CTI GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 337
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

BLAST of CsGy2G004910 vs. NCBI nr
Match: TYJ96459.1 (putative pectinesterase 66 [Cucumis melo var. makuwa])

HSP 1 Score: 622 bits (1605), Expect = 7.24e-224
Identity = 301/337 (89.32%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ++PPFLLLFTLFNAVVFGFEAVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK C I GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 337
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

BLAST of CsGy2G004910 vs. NCBI nr
Match: XP_016901459.1 (PREDICTED: probable pectinesterase 66 [Cucumis melo])

HSP 1 Score: 605 bits (1561), Expect = 1.11e-216
Identity = 302/367 (82.29%), Postives = 317/367 (86.38%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ++PPFLLLFTLFNAVVFGFEAVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVY------KEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATF 120
           NNN WIKIQINPGVY      +EKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATF
Sbjct: 61  NNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATF 120

Query: 121 TSRPPNIIVRGITFE------------------------NSFNLREAPELFSCDDGTYIT 180
           TSRPPNII RGITFE                        NSFNL EAPELF CDDGTYIT
Sbjct: 121 TSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYIT 180

Query: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMI 240
           QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIFGSGQSVY+DC+I
Sbjct: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLI 240

Query: 241 NVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAI 300
           NVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSGKALLGRAYGPFSRVIFKDAI
Sbjct: 241 NVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAI 300

Query: 301 MGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFI 337
           MG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRVPWAKTLDAS L  FSVESFI
Sbjct: 301 MGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFI 360

BLAST of CsGy2G004910 vs. ExPASy TrEMBL
Match: A0A0A0LH00 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1)

HSP 1 Score: 699 bits (1803), Expect = 2.31e-254
Identity = 339/340 (99.71%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQV+PPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV
Sbjct: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT
Sbjct: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340
           GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340

BLAST of CsGy2G004910 vs. ExPASy TrEMBL
Match: A0A5A7U1G9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001480 PE=4 SV=1)

HSP 1 Score: 624 bits (1610), Expect = 6.07e-225
Identity = 302/337 (89.61%), Postives = 316/337 (93.77%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ++PPFLLLFTLFNAVVFGFEAVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK CTI GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCTITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 337
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

BLAST of CsGy2G004910 vs. ExPASy TrEMBL
Match: A0A5D3BBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001020 PE=4 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 3.51e-224
Identity = 301/337 (89.32%), Postives = 315/337 (93.47%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ++PPFLLLFTLFNAVVFGFEAVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNN WIKIQINPGVYKEKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNQWIKIQINPGVYKEKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           II RGITFENSFNL EAPELF CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIARGITFENSFNLEEAPELFVCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFF +CYIEGAIDFIFGSGQSVY+DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFV
Sbjct: 181 GRHFFQNCYIEGAIDFIFGSGQSVYKDCLINVNVASLPQVHQGFITAQGRRSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FK C I GSGKALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCT
Sbjct: 241 FKGCAITGSGKALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIP 337
           GPGASTS RVPWAKTLDAS L  FSVESFINQDGWIP
Sbjct: 301 GPGASTSTRVPWAKTLDASQLRRFSVESFINQDGWIP 337

BLAST of CsGy2G004910 vs. ExPASy TrEMBL
Match: A0A1S4DZS3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 5.38e-217
Identity = 302/367 (82.29%), Postives = 317/367 (86.38%), Query Frame = 0

Query: 1   MQVRPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ++PPFLLLFTLFNAVVFGFEAVN AR F LT+QSVV+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVY------KEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATF 120
           NNN WIKIQINPGVY      +EKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATF
Sbjct: 61  NNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATF 120

Query: 121 TSRPPNIIVRGITFE------------------------NSFNLREAPELFSCDDGTYIT 180
           TSRPPNII RGITFE                        NSFNL EAPELF CDDGTYIT
Sbjct: 121 TSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKCTQNSFNLEEAPELFVCDDGTYIT 180

Query: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMI 240
           QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIFGSGQSVY+DC+I
Sbjct: 181 QAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKDCLI 240

Query: 241 NVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAI 300
           NVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSGKALLGRAYGPFSRVIFKDAI
Sbjct: 241 NVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSGKALLGRAYGPFSRVIFKDAI 300

Query: 301 MGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFI 337
           MG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRVPWAKTLDAS L  FSVESFI
Sbjct: 301 MGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRVPWAKTLDASQLRRFSVESFI 360

BLAST of CsGy2G004910 vs. ExPASy TrEMBL
Match: A0A6J1G5A0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1)

HSP 1 Score: 469 bits (1208), Expect = 5.17e-164
Identity = 225/318 (70.75%), Postives = 256/318 (80.50%), Query Frame = 0

Query: 23  AVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIP 82
           A+N  R   L IQSV++VDKSG GNF+T+QAAIDSVP NNN W+KIQ+NPG Y+E VTIP
Sbjct: 5   AMNFTRALDLKIQSVIVVDKSGKGNFKTIQAAIDSVPLNNNKWVKIQVNPGEYREMVTIP 64

Query: 83  LEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFS 142
             K FIYL+G   S TVITF+ HQQT+TSATF S   NII RGITFENSFNL  AP+LF 
Sbjct: 65  STKSFIYLQGGSRSTTVITFNGHQQTNTSATFNSMADNIIARGITFENSFNLANAPQLFD 124

Query: 143 CDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQ 202
              GT+ TQA+AARIYGDKSAF +CGFKG+QDTLWDV+GRH+F  CYIEGAIDFIFG+GQ
Sbjct: 125 KITGTFFTQALAARIYGDKSAFISCGFKGFQDTLWDVKGRHYFKSCYIEGAIDFIFGNGQ 184

Query: 203 SVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFS 262
           S+YE C+I VNV  LP+V QGYITAQ RQSA DPSGFVF++CTI GSGK  LGRAYGPFS
Sbjct: 185 SIYEGCLIYVNVGLLPEVNQGYITAQGRQSANDPSGFVFQQCTITGSGKGFLGRAYGPFS 244

Query: 263 RVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLT 322
           RVIFKD  MG VVAPEGW AWHFKG+E NF+Y+E N  G G+S S RVPWA T   S L+
Sbjct: 245 RVIFKDTNMGKVVAPEGWDAWHFKGQETNFVYIESNSRGIGSSPSKRVPWAMTQGNSQLS 304

Query: 323 GFSVESFINQDGWIPTVL 340
           G+SV+SFINQDGWIP  L
Sbjct: 305 GYSVKSFINQDGWIPNFL 322

BLAST of CsGy2G004910 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.2e-82
Identity = 155/330 (46.97%), Postives = 211/330 (63.94%), Query Frame = 0

Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           +++ TLF   V+G+ A  +   + +T      VD +G GNF TVQ+AIDS+ P N++WI+
Sbjct: 14  MVVMTLF---VYGYSAAAEQIAYTIT------VDLNGGGNFTTVQSAIDSISPPNHNWIR 73

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           +    G+Y+EKVTIP EK FIYL+G     TVI +DDHQ TD SATFT+   +I++ GIT
Sbjct: 74  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 133

Query: 128 FENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSH 187
           F+N++N+   P     ++   I  A+AAR+ GD+    +  F G QDTL+D +GRH++  
Sbjct: 134 FKNTYNI--VP-----NNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKR 193

Query: 188 CYIEGAIDFIFGSGQSVYEDCMINVNVA-SLPQVYQGYITAQSRQSAADPSGFVFKECTI 247
           C I G IDFIFG GQS++++C +N+ +    P    G ITA  R S +D  GFVF +CT+
Sbjct: 194 CIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTV 253

Query: 248 KGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAST 307
            G GK LLGRA+G  +RVIF  + +  VV P GW AW  KG E +  +VE  CTG GA T
Sbjct: 254 TGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADT 313

Query: 308 SMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           S RVPW K L  S + GF+  SFI+QDGWI
Sbjct: 314 SQRVPWLKKLSLSEVDGFASVSFIDQDGWI 327

BLAST of CsGy2G004910 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.8e-81
Identity = 145/299 (48.49%), Postives = 192/299 (64.21%), Query Frame = 0

Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           VIVD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+TIP +KPFI + GA   +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARI 157
           T + +DDH     S TF +   N +V+ ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 158 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 217
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 218 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 277
                GYITAQ R +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 278 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            GW+ W+++G E+   Y E  C G G++TS R  W K L AS +   +  SFIN+ GW+
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWV 323

BLAST of CsGy2G004910 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.7e-80
Identity = 143/302 (47.35%), Postives = 188/302 (62.25%), Query Frame = 0

Query: 35  QSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGAD 94
           Q  V VD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 95  SSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIA 154
              T + +DDH     S TF++   N +V+ ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 155 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNV 214
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 215 ASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSV 274
             L     GYITAQ R +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 275 VAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDG 334
           V PEGW AW+F G E   ++ E  C G GA+   RV W K L  S +   +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 328

Query: 335 WI 337
           W+
Sbjct: 331 WV 328

BLAST of CsGy2G004910 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.7e-77
Identity = 142/305 (46.56%), Postives = 191/305 (62.62%), Query Frame = 0

Query: 34  IQSVVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGA 93
           I   +IV+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 94  DSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAI 153
               T+I + DHQ T+TSATFTS P NII+ GITF+N +N+            +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNI--------ASSSSPTKPAV 156

Query: 154 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVN 213
           AA + GDK A  +  F G+QDTL+D  GRH++  C I G IDFIFG  QS++E C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 214 VASLP--QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIM 273
           V   P  +VY G ITAQ R S  D  GFVFK+CT+ GSGKALLGRA+  +SRVIF  ++ 
Sbjct: 217 VGIYPPNEVY-GTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 276

Query: 274 GSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFIN 333
              + P GW AW  KG+E +  +VE  CTG GA TS RVPW        +  F+  +FI+
Sbjct: 277 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 332

Query: 334 QDGWI 337
           ++GW+
Sbjct: 337 EEGWL 332

BLAST of CsGy2G004910 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.0e-68
Identity = 128/245 (52.24%), Postives = 161/245 (65.71%), Query Frame = 0

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTY-ITQAIAAR 157
           T IT++ H  TD S+TFTS P +I+VR ++  N++N      L   +  ++ I  A+A  
Sbjct: 48  TTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIKPAVAIS 107

Query: 158 IYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVAS 217
           +YGDKSAF+NC F G QDT+WD  GRH F +CYIEGAIDFIFGSGQSVYEDC IN    +
Sbjct: 108 VYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGA 167

Query: 218 L-PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVV 277
           L  +V  GYITAQ R S +DPSGFVF   ++ GS    LGRAYGPFSRVIF    + SVV
Sbjct: 168 LASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVV 227

Query: 278 APEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHL-TGFSVESFINQDG 337
            PEGWY+WH+ G E +F Y E  C G G+  S RVPW   L + +    FS+ +FI+QD 
Sbjct: 228 HPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQ 287

Query: 338 WIPTV 340
           WI  +
Sbjct: 288 WISNI 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4PSQ51.7e-8146.97Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
Q3E9D39.5e-8048.49Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
Q4PSN08.1e-7947.35Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
O644793.8e-7646.56Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
O049531.4e-6752.24Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
KGN61008.14.78e-25499.71hypothetical protein Csa_021278 [Cucumis sativus][more]
XP_031736683.11.17e-24893.65probable pectinesterase 66 [Cucumis sativus][more]
KAA0048077.11.25e-22489.61putative pectinesterase 66 [Cucumis melo var. makuwa][more]
TYJ96459.17.24e-22489.32putative pectinesterase 66 [Cucumis melo var. makuwa][more]
XP_016901459.11.11e-21682.29PREDICTED: probable pectinesterase 66 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0LH002.31e-25499.71Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G034610 PE=4 SV=1[more]
A0A5A7U1G96.07e-22589.61Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00148... [more]
A0A5D3BBR93.51e-22489.32Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00102... [more]
A0A1S4DZS35.38e-21782.29Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499379 PE=4 SV=1[more]
A0A6J1G5A05.17e-16470.75Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450826 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47280.11.2e-8246.97Pectin lyase-like superfamily protein [more]
AT5G18990.16.8e-8148.49Pectin lyase-like superfamily protein [more]
AT3G24130.15.7e-8047.35Pectin lyase-like superfamily protein [more]
AT2G19150.12.7e-7746.56Pectin lyase-like superfamily protein [more]
AT5G26810.11.0e-6852.24Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 38..332
e-value: 4.6E-61
score: 206.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 31..339
e-value: 1.7E-102
score: 345.1
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 18..337
NoneNo IPR availablePANTHERPTHR31321:SF82PECTINESTERASEcoord: 18..337
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 190..199
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 33..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G004910.2CsGy2G004910.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity