CsGy2G002760 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G002760
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationGy14Chr2: 1813729 .. 1816685 (+)
RNA-Seq ExpressionCsGy2G002760
SyntenyCsGy2G002760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAATCAAAATCATAGACTGTGGGCGCACACAAATCCAATCCTCCAATTACCCACCAAAATTTCTCTCCCACTTTCTTCGATGGCTAATTTTCTCAAACCCCACTTACTATATATTCATTTTCCTTTCATTTCCACCTCCCGTGTTTCAGAATCGCCTTGTTGTTTTGATTCTTTTCTAGCCTCTTCATTCTGTCAATGGCAATATCTAAAGGGAAGTACATCAAGTTACAGGGTAGGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTTTCTCTTCCGTCCGGCATACAGCATTTCTAAGGTAATCTGGTAATGCAATGGGTGTGATTCTTTAAGCTTGTTTGTTACAGGAATCAACTGATTTTGATTTCCATTTTGGTAATATTGTTGTTGTAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGGTAAGTTTTTAGAATTGTGGTTTTTTTTTTTTGGAAAAAAAAAAGGAAATTTTGTTTTGTTAAAATGGTTGGCTGATATTGGAATGGCGTTGGACAGTCCAAAGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCCCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCGAGAGTGTGGATAGCAGGTATGTCTTTATCATTTTCTTTGTTGTCCTTTTACTAACAGAAACAGAACTTGTCAATTATTGACTATGAACTAACTCTGGTGGGGCTTACACTATGACACATTTAAAAGTGTAAGAAAAAGTAATCTCAAAGAGAGTAAATTTGAACTAAACTCATATTTTAAAGATGATTTATCTATATTTAGTCGTTTCTCATAAGTTATCTCTTTAGGAATTGATTGGTCGGTTCGGTTCTTGAAAAATCAGTTCGGTAAAAGTCCAATCTGAACTGACTACAATAGAATGGTTATGTATAATAGAAGGGTATAAAGTAGAACAGTACAATTACAATGAACAAGGCAAACTTATAACTTTTTTCTGTACAATGCCATGTCCACAGGGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGAAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCCATAGGTAAATAATTCAAAACTTTCATATCACCACGGCTTATTTAGAAATACTTTTCAAAACAGTTTTTGAAAACATGTTCGAACCTCTGTTTTTTGCTAGTTGTTTTATTTTGTCTGTGTTAGTGATTTCATTCAATATATCAATCTTTACAATGCATTCAACTGTAGTTATATTGAATAATTCTTCAAAATAGAGTTGCAACCACAACATAATTCTCAAACAAATCTTTGTTGGACTAAACAAACATTCGCGAAAAATTAGTTAAAATTCATGTGGGATTTTGTTCATTATTCTTGTTTTTGGCCCAAACCAAAAAGGGCTTTGGTGAATGAGCTATTCATTTGCTGCTTGTTGTTTTGTTGATGTCACTGGCATTTTGGTCTTAATACATTTTGGTTAGTGTTTGATTGTTAGATTATTAGACTTGTCTCGTTTATTATCATTAACAAAGAGGATTGTTTCTGTTAAAAAAAACATTTTGGTAATGATATTCACAATTCCACACAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAATATGATGCGCATTATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTTCTCATGTATTTGTAAGATTCTACTTTGACAATCTCTCAGACTCATCTACCCTCTTCTCTAGTTTCTCTCTGAATCGATACCAATGGCAATGCAGGTCCGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCAGCTGCAAAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTATCTGAATGTGGCAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATGCTACCTTAGACCCTACAAGTTTACATGGTTAGTCCTTTTTACTTTTCCTCCCTATTTAACTTTAGCATTAGAAGATTGGTTACATCTAAATTATCCTCCCTAATCTTGTTTTTGATATGTAGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTGATAAATATATTTAACCATGAGGTGAGTTACATTTTTTGAAAGATTATTCTGTATACATCATTATAAAAATATATATTTTTTCCTTATTGTGCAAAATAAATCTACCAATAAAACTAGAAATAGTGTATATATTTCCTTTAAATGTAAATTGAGTACTTTTCTTTTTTAAACAAATTAATTTGTGTTGAATTTGTATATTTACATGGAGACATTTTGAAGTGCAATAGTTTTTTCTTTTTATGATCATTTGTTATTTTTCTTGAACATAAAAGAACAATTGGCAATAATAAATTTCAAGAAATTATAAATGAATAATGTAACATAATTTATCCATTTATCCTCGAATATTTGTATGATTTAAAGAGATTATTTCCAAATTGCAGGGTGTAATGAAAGCACTTTGTTGGGGGAAGAAGACAATTGGAAGACCACATATGATTTTCTTTTTTAACATTATATTTTATAGCTTAATTTGTTATCCATCCTCTTTAATTTTACATATTTTGTTGTTAATATAATATAGAAAAAGTTCATTTTTTTAAAGCAGAAAATTTCTTTTAAGTTTAAATTTTACATGTTCCATTTAAGGTTATTTTTTTACATTGATAAACATTGATCAATTAAATCTATTTGTATCTATTATTGATATAATTTAAAATTTTGT

mRNA sequence

GAGAAAATCAAAATCATAGACTGTGGGCGCACACAAATCCAATCCTCCAATTACCCACCAAAATTTCTCTCCCACTTTCTTCGATGGCTAATTTTCTCAAACCCCACTTACTATATATTCATTTTCCTTTCATTTCCACCTCCCGTGTTTCAGAATCGCCTTGTTGTTTTGATTCTTTTCTAGCCTCTTCATTCTGTCAATGGCAATATCTAAAGGGAAGTACATCAAGTTACAGGGTAGGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTTTCTCTTCCGTCCGGCATACAGCATTTCTAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGTCCAAAGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCCCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCGAGAGTGTGGATAGCAGGGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGAAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCCATAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAATATGATGCGCATTATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTTCTCATGTATTTGTCCGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCAGCTGCAAAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTATCTGAATGTGGCAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATGCTACCTTAGACCCTACAAGTTTACATGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTGATAAATATATTTAACCATGAGGGTGTAATGAAAGCACTTTGTTGGGGGAAGAAGACAATTGGAAGACCACATATGATTTTCTTTTTTAACATTATATTTTATAGCTTAATTTGTTATCCATCCTCTTTAATTTTACATATTTTGTTGTTAATATAATATAGAAAAAGTTCATTTTTTTAAAGCAGAAAATTTCTTTTAAGTTTAAATTTTACATGTTCCATTTAAGGTTATTTTTTTACATTGATAAACATTGATCAATTAAATCTATTTGTATCTATTATTGATATAATTTAAAATTTTGT

Coding sequence (CDS)

ATGGCAATATCTAAAGGGAAGTACATCAAGTTACAGGGTAGGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTTTCTCTTCCGTCCGGCATACAGCATTTCTAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGTCCAAAGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCCCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCGAGAGTGTGGATAGCAGGGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGAAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCCATAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAATATGATGCGCATTATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTTCTCATGTATTTGTCCGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCAGCTGCAAAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTATCTGAATGTGGCAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATGCTACCTTAGACCCTACAAGTTTACATGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTGATAAATATATTTAA

Protein sequence

MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI*
Homology
BLAST of CsGy2G002760 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.3e-117
Identity = 207/286 (72.38%), Postives = 233/286 (81.47%), Query Frame = 0

Query: 3   ISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPP----ETSHHRFSSVRH 62
           ++K ++ + Q RKWST  L  + M  +L +   ML+A   FS P    E+S    S  R 
Sbjct: 1   MAKLRHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 63  TAF-LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 122
            A   S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDS+TG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 123 ESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 182
           +S DSRVRTSSG FL RG+DKII+ IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYD
Sbjct: 121 KSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYD 180

Query: 183 YFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVK 242
           YFVDE+N K GGQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVK
Sbjct: 181 YFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVK 240

Query: 243 PKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           P+MGDALLFWSM+PDATLDPTSLHG CPVIRGNKWS TKWMHV +Y
Sbjct: 241 PRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of CsGy2G002760 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.1e-108
Identity = 188/270 (69.63%), Postives = 217/270 (80.37%), Query Frame = 0

Query: 22  SKMIMALVLALGFFMLIALRFFSPPETSHHRFSS--------VRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F      S++  SS        VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD KTG+S DSRVRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMA 201
           RG+DK IR IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYDYF+DEYN + GGQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDA 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGGLSVKPKMGDALLFWSM PDA
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           TLDP+SLHG C VI+GNKWS TKW+ V +Y
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

BLAST of CsGy2G002760 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 8.2e-101
Identity = 178/289 (61.59%), Postives = 219/289 (75.78%), Query Frame = 0

Query: 5   KGKYIKLQGRK-WSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR---------FS 64
           K K ++ + RK +ST   + +++ L + L   +L+ L  FS P T+              
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVIL---ILVGLGIFSLPSTNKTSSMPMDLTTIVQ 63

Query: 65  SVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 124
           +++      D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD 
Sbjct: 64  TIQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDV 123

Query: 125 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 184
           KTG+S+DSRVRTSSG FLNRG D+I+  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ 
Sbjct: 124 KTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEP 183

Query: 185 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 244
           H+DYF DE+N++KGGQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GL
Sbjct: 184 HHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGL 243

Query: 245 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFWSMKPDA+LDP+SLHG CPVI+GNKWS TKW HV +Y
Sbjct: 244 SVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

BLAST of CsGy2G002760 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 2.6e-99
Identity = 171/289 (59.17%), Postives = 216/289 (74.74%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSS----- 60
           MA    ++++ Q RK  +       + ++L +   +L+ L   S P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180
           KTG S DSRVRTSSG FL RG D+++  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 240
           HYDYF+DE+N K GGQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFW+M+PDA+LDP+SLHG CPV++GNKWS TKW HV ++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

BLAST of CsGy2G002760 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.5e-67
Identity = 116/207 (56.04%), Postives = 157/207 (75.85%), Query Frame = 0

Query: 77  ISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQ 136
           +SW PR F+Y  FLS EEC + I LAK  +EKS V D+ +GESV+S VRTSSGMFL++ Q
Sbjct: 59  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 118

Query: 137 DKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLL 196
           D I+ N+E ++A +TF+P E+GE +QILHYE GQKY+ H+DYF D+ N++ GG R+AT+L
Sbjct: 119 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 178

Query: 197 MYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLD 256
           MYLS+VE+GGETVFP  KG  + +   +  +EC K G +VKP+ GDALLF+++ P+AT D
Sbjct: 179 MYLSNVEKGGETVFPMWKGKATQLK-DDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 238

Query: 257 PTSLHGACPVIRGNKWSCTKWMHVDKY 284
             SLHG+CPV+ G KWS T+W+HV  +
Sbjct: 239 SNSLHGSCPVVEGEKWSATRWIHVKSF 264

BLAST of CsGy2G002760 vs. NCBI nr
Match: XP_011648735.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical protein Csa_019368 [Cucumis sativus])

HSP 1 Score: 587 bits (1513), Expect = 9.91e-212
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA
Sbjct: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284
           GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284

BLAST of CsGy2G002760 vs. NCBI nr
Match: XP_008456388.1 (PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo])

HSP 1 Score: 570 bits (1469), Expect = 5.07e-205
Identity = 275/284 (96.83%), Postives = 279/284 (98.24%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+SKGKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR  SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG+SV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284
           GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHV+KYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 284

BLAST of CsGy2G002760 vs. NCBI nr
Match: XP_038889689.1 (probable prolyl 4-hydroxylase 3 [Benincasa hispida])

HSP 1 Score: 555 bits (1431), Expect = 3.65e-199
Identity = 268/288 (93.06%), Postives = 278/288 (96.53%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSH----HRFSSV 60
           MA+SKGKY K+QG+KWSTF+LSKMIMALVLALGFFML+ALRFFSPPETSH    H  +SV
Sbjct: 1   MALSKGKYTKIQGKKWSTFELSKMIMALVLALGFFMLLALRFFSPPETSHRNLPHHLASV 60

Query: 61  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120
           RH+A  SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT
Sbjct: 61  RHSAVESDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 120

Query: 121 GESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180
           G+SVDSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY
Sbjct: 121 GKSVDSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 180

Query: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 240
           DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAA+GNFSSVPWWNELSECGKGGLSV
Sbjct: 181 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAARGNFSSVPWWNELSECGKGGLSV 240

Query: 241 KPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284
           KPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHV+KYI
Sbjct: 241 KPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 288

BLAST of CsGy2G002760 vs. NCBI nr
Match: XP_016901368.1 (PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis melo])

HSP 1 Score: 546 bits (1406), Expect = 2.04e-195
Identity = 262/284 (92.25%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+S GKYIKLQG+KWSTFQLSKMIMALVLALGFFML AL FFSPPETSHHR SSVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TG+SV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DS VRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HV+KYI
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 284

BLAST of CsGy2G002760 vs. NCBI nr
Match: XP_011650099.2 (probable prolyl 4-hydroxylase 3 [Cucumis sativus] >XP_031736348.1 probable prolyl 4-hydroxylase 3 [Cucumis sativus])

HSP 1 Score: 539 bits (1389), Expect = 7.95e-193
Identity = 257/282 (91.13%), Postives = 273/282 (96.81%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+S  KYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRF SPPETSHHRFSSVRHTA
Sbjct: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD++TG++V
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           +  VRTSSGMFLNRGQDKI+ NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD+F 
Sbjct: 121 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DE+N+K+ GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELS+CGKGGLSVKPKM
Sbjct: 181 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDK 282
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HV++
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 282

BLAST of CsGy2G002760 vs. ExPASy TrEMBL
Match: A0A1S3C2P6 (probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 2.45e-205
Identity = 275/284 (96.83%), Postives = 279/284 (98.24%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+SKGKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR  SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG+SV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284
           GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHV+KYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 284

BLAST of CsGy2G002760 vs. ExPASy TrEMBL
Match: A0A1S4DZG7 (probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=1)

HSP 1 Score: 546 bits (1406), Expect = 9.86e-196
Identity = 262/284 (92.25%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+S GKYIKLQG+KWSTFQLSKMIMALVLALGFFML AL FFSPPETSHHR SSVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TG+SV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DS VRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HV+KYI
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 284

BLAST of CsGy2G002760 vs. ExPASy TrEMBL
Match: A0A0A0LFF5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009620 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 8.85e-193
Identity = 258/259 (99.61%), Postives = 258/259 (99.61%), Query Frame = 0

Query: 26  MALVLALGFFMLIALRFFSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 85
           MALVLALGFFMLIALRF SPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV
Sbjct: 1   MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 60

Query: 86  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 145
           YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK
Sbjct: 61  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 120

Query: 146 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 205
           RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG
Sbjct: 121 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 180

Query: 206 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 265
           GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP
Sbjct: 181 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 240

Query: 266 VIRGNKWSCTKWMHVDKYI 284
           VIRGNKWSCTKWMHVDKYI
Sbjct: 241 VIRGNKWSCTKWMHVDKYI 259

BLAST of CsGy2G002760 vs. ExPASy TrEMBL
Match: A0A1S3C367 (probable prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496331 PE=4 SV=1)

HSP 1 Score: 521 bits (1342), Expect = 6.99e-186
Identity = 258/289 (89.27%), Postives = 264/289 (91.35%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+SKGKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR  SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG+SV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGA-----CPVIRGNKWSCTKWMHVDKYI 284
           GDALLFWSMKPDATLDPTSLHG      C       W  + W   D ++
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGCNENTLC-------WGRSNWKTTDDFL 282

BLAST of CsGy2G002760 vs. ExPASy TrEMBL
Match: A0A5D3BDQ0 (Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001180 PE=4 SV=1)

HSP 1 Score: 520 bits (1339), Expect = 7.16e-186
Identity = 254/262 (96.95%), Postives = 257/262 (98.09%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSSVRHTA 60
           MA+SKGKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR  SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG+SV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHG 262
           GDALLFWSMKPDATLDPTSLHG
Sbjct: 241 GDALLFWSMKPDATLDPTSLHG 262

BLAST of CsGy2G002760 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 424.1 bits (1089), Expect = 8.9e-119
Identity = 207/286 (72.38%), Postives = 233/286 (81.47%), Query Frame = 0

Query: 3   ISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPP----ETSHHRFSSVRH 62
           ++K ++ + Q RKWST  L  + M  +L +   ML+A   FS P    E+S    S  R 
Sbjct: 1   MAKLRHSRFQARKWSTLML-VLFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 63  TAF-LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 122
            A   S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDS+TG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 123 ESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 182
           +S DSRVRTSSG FL RG+DKII+ IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYD
Sbjct: 121 KSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYD 180

Query: 183 YFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVK 242
           YFVDE+N K GGQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVK
Sbjct: 181 YFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVK 240

Query: 243 PKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           P+MGDALLFWSM+PDATLDPTSLHG CPVIRGNKWS TKWMHV +Y
Sbjct: 241 PRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

BLAST of CsGy2G002760 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 7.6e-110
Identity = 188/270 (69.63%), Postives = 217/270 (80.37%), Query Frame = 0

Query: 22  SKMIMALVLALGFFMLIALRFFSPPETSHHRFSS--------VRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F      S++  SS        VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD KTG+S DSRVRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMA 201
           RG+DK IR IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYDYF+DEYN + GGQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDA 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGGLSVKPKMGDALLFWSM PDA
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           TLDP+SLHG C VI+GNKWS TKW+ V +Y
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

BLAST of CsGy2G002760 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 368.2 bits (944), Expect = 5.8e-102
Identity = 178/289 (61.59%), Postives = 219/289 (75.78%), Query Frame = 0

Query: 5   KGKYIKLQGRK-WSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR---------FS 64
           K K ++ + RK +ST   + +++ L + L   +L+ L  FS P T+              
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVIL---ILVGLGIFSLPSTNKTSSMPMDLTTIVQ 63

Query: 65  SVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 124
           +++      D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD 
Sbjct: 64  TIQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDV 123

Query: 125 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 184
           KTG+S+DSRVRTSSG FLNRG D+I+  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ 
Sbjct: 124 KTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEP 183

Query: 185 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 244
           H+DYF DE+N++KGGQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GL
Sbjct: 184 HHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGL 243

Query: 245 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFWSMKPDA+LDP+SLHG CPVI+GNKWS TKW HV +Y
Sbjct: 244 SVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

BLAST of CsGy2G002760 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 363.2 bits (931), Expect = 1.9e-100
Identity = 171/289 (59.17%), Postives = 216/289 (74.74%), Query Frame = 0

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFSS----- 60
           MA    ++++ Q RK  +       + ++L +   +L+ L   S P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180
           KTG S DSRVRTSSG FL RG D+++  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 240
           HYDYF+DE+N K GGQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFW+M+PDA+LDP+SLHG CPV++GNKWS TKW HV ++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

BLAST of CsGy2G002760 vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 8.2e-80
Identity = 147/220 (66.82%), Postives = 171/220 (77.73%), Query Frame = 0

Query: 22  SKMIMALVLALGFFMLIALRFFSPPETSHHRFSS--------VRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F      S++  SS        VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLN 141
           VE ISWEPRA VYHNFL  EEC YLI LAKPHMEKSTVVD KTG+S DSRVRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMA 201
           RG+DK IR IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYDYF+DEYN + GGQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 234
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGG
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LN201.3e-11772.38Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
F4JZ241.1e-10869.63Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S... [more]
F4JNU88.2e-10161.59Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=... [more]
Q24JN52.6e-9959.17Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1[more]
Q8L9703.5e-6756.04Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_011648735.29.91e-212100.00probable prolyl 4-hydroxylase 3 [Cucumis sativus] >KAE8651549.1 hypothetical pro... [more]
XP_008456388.15.07e-20596.83PREDICTED: probable prolyl 4-hydroxylase 3 isoform X2 [Cucumis melo][more]
XP_038889689.13.65e-19993.06probable prolyl 4-hydroxylase 3 [Benincasa hispida][more]
XP_016901368.12.04e-19592.25PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis melo][more]
XP_011650099.27.95e-19391.13probable prolyl 4-hydroxylase 3 [Cucumis sativus] >XP_031736348.1 probable proly... [more]
Match NameE-valueIdentityDescription
A0A1S3C2P62.45e-20596.83probable prolyl 4-hydroxylase 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034963... [more]
A0A1S4DZG79.86e-19692.25probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103496316 PE=4 SV=... [more]
A0A0A0LFF58.85e-19399.61Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G... [more]
A0A1S3C3676.99e-18689.27probable prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034963... [more]
A0A5D3BDQ07.16e-18696.95Putative prolyl 4-hydroxylase 3 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
AT1G20270.18.9e-11972.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.17.6e-11069.632-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G35810.15.8e-10261.592-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17720.11.9e-10059.172-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G66060.28.2e-8066.822-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 81..279
e-value: 5.2E-64
score: 228.7
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 74..280
e-value: 3.2E-80
score: 270.7
NoneNo IPR availablePANTHERPTHR10869:SF169PROLYL 4-HYDROXYLASE 3-RELATEDcoord: 4..283
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 161..279
e-value: 1.3E-20
score: 74.0
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 4..283
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 157..280
score: 12.145268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G002760.2CsGy2G002760.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen