CsGy2G002280 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G002280
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionNEDD8 ultimate buster 1
LocationGy14Chr2: 1500637 .. 1506025 (-)
RNA-Seq ExpressionCsGy2G002280
SyntenyCsGy2G002280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAATAGAATATTTTACTTTAAAGTTAATAAAATAACTATTATGCAATCAATTAGAAGATATGAATATATTTGAACATTTTCAAGCAATTATGTTAGGAATGTTACTCATAATTAAAAAAAAAAAATCAAATGAGCAATAAAACAAAATTTATGGGAAGTAAGATTGAACAAATGTAGAACTAAATCAAAGATAAAATTCTGATCTTTAAAAGGTGGATAGGCTAATGCTCAAGGCCCAAGCCGAACACGAAAACTCGCGTAAACAAACTTGAAAGCGGAGAAATTTGATCCAAATTGTCCCGAAATTCCAAGACTTGACCAACAAGGAAACCTCATCGTACCTTTTAAAGCCTCCTTTCATTAATTCCAATCATACCCACCTCGATTTTCTCCATCTTCTCTCATCTGAAGGATCAAAAGACCACTCAGTGACTTTCCCACCCTCCCTTTTTACAGTCCCAATTTCTATTTTGGTTCAGTTTTCATGGCCAAACTCAAGATTTCAGGTCCATGGACTGGTGTGTTGGATGTAGAACTCGATAATTGGACTGTTGTCATGTTGAGGGAAGAAATTGCAAGACGATCGAACTGTGGTGTAGAGTCTATCAATTTAATTTCTGGTGGCAGAATCTTGAAAGATGGTGATGGAAGCGAGAAGCTGGTTCATTTGGGTCTTAAGAACAACTCTAGGATTCTTGCGAGACGGGTTGCTGCTGAAGAGGGGAAGGCACTCAAGAATGAATTGATGGCTGAGGAAGAACGCTCTTCCAGGCTTGCTCGAGTCAAGTGATTCTTTGACCTTTTATTTCTGTAGTTATGTTTGTATCTTCTCTTATAACGATGTTTGTTAGAATTATGTGCATTGTCATTGCTGTGTTCATGTGTTTCAACTGGGTTTGCTTTGTTTGCTTGTGTTTCAATAGATTTTGCTATTTTGGGAAGCTTGGTTATGTAGACTGATGATTCCATGTAGAAATGGAAAAAAAATTATTTGGAGTTTTTGGGGTTAAATGGGCTTGGTTTTCACTGTCAAGTTTTTTTCTTGAAGTAATAGTGGAAATAGGATTTTAAAACCAAAATGAAAGATGGTTTTGGAAAGATGCTTAAGTTCTTCTATGGTGATATGATGAGGCTTTAGCAGTTGATTGTGGAAGCAAAGCATCTAGTTCAGGAATGAAAAGCAAAATTTATTTGTAAGATGCAATGTGGCATAATGAAGCAAATGACAGTCAACTTTTGGAGAGTGTTTTTCCCCACGGGAGGGTCATGAGTAGTAGTTCTGAAGATTTTAAAGACATTTCTTTTGTATTGTTGATGTCGATGTTGAATGTTTGTTTTACGTCGTTTAGTATCTACCTGATTTTCTGGCATCTTCTGAACTTACAGGGCAGCTGCCACTGCTCTTGCTGAGAGGCATGCTAATGGTTTTCTCCCAGTTGAAGATTTCAATATGGAACTGGAGGATCAAAGTGGCCAGAAAGTTAATTTTGGGTCTGAAACTGACCAAAAGTAAGTAATAAATCATTCTCAACATGTGAATTCATGTTTACTCATGTCTAATGTTATAAATATATTTGTTGTACATTCAACAGGGCAATCATGATGGGTCTTATGCTTCATGCAAACGCAAAGCAGCTGATAAGAAAAGGAAACTACAAAGATGCATTAGAAGTACTCGTCATGGGAGAGGTCTGAGCTTGGTTAATTATGTTAATCAGTCTTCAATATTAGCTTCTAAATTTTAGCTGTTTTCCCCCTTTAAAGAAATAGACAAGATCGAAATGATTTTAGATAAACTTGAAGATTAAACCATTATTGAGGACGATAAACACCTTCCTCAAAAGTAAAGGATAAAATAAATATATCACTCCAGTATGGTTGATCTGCTCTATGTGGGTGACAACTGAGTTTGAAGTGCTGTGCTTTGATGATCTTTATTAGCCAATGGTTCCAAATAAATACATGACTGTTCAATTCTACTTTTGCTTTCTTTACCTTTAAATTGATGGTAAAAGAAAGCACTATTTATGGTTTGATTATTTCAAGTCGTTGTGCCAGATACTGATATCAGCATACTTTTATTTACTATGAAAGAATACTAATCAATGTGGATTATGTTCTTGTAGGAGGCTTTTACTCTATGCAATCCAAAACTTATTGAGGTGAATGACTCCTCCTTTATCATTAGTTCCTCCCAACCACCACCAAGACTCATTGCTGAAGTTATTTATACATGTAAAAGAAAGAGAAATATAAGTTTCTCAAACTATAAACTATTCATTTATAACATTGCAACTGAAGGTAGTATCAGATCTTTTAAATATTATTAAGGTTTGTGTTATGATTGTGATATGATTTGAGGCCTTGGATTGAGACTACATGAAAATACAAAAAATTTAATGATTTTCCTTATCTTCTGTATGTGACCGATGGTGGTGTTTCCATCACGGTTGTATCAATGTAGTTCTTTTTTAAAAAATAAATAAATAAATAAAATTGATGTGGTTTTTAATTTTCTTTAACTCAGAATATCTGTATAGCTTGCTGACTAAGAATGAGCCTTCTTCTCTATCAGTTTGTTGACAACGTCTCAATACTTCAAATAGACATGGTTTGGTGCTATTTCATGCTAAAAGACATCAAGTGGCTCTCAGATGCCGGATTACGCCTTGCAAAGGCTAGAGAAGGAATCGAACGTGCCCATGGGAAGGACTCTTCCCGCGTTCGAATCCTTCAAGCTGGTTGCCATCCTGAGCTTGCCTTGTAAGTCCACTGCATGTTATTCCCAACAGTTTGAGGGATGGTTTTCAGAAACAAGCTAATTACGTTTTATCATAATGTTAGACATTTGAGACTGGAGCTGCTGGAGGGGGTGGCTGCATATCACAGCTGTAGGTTTGATAAGTCTCGGGAAGCAATCAACTCAGCACGAGCAAAATATTTTCAGGTTAGCCTATCATTTTACAGAATTGGGAGGATCAAGCTTGAGTATGTTATAGAATTGAATGGTGTACTAGCGGAGATATCAGGCCTACTATATGTTGAGCAATTTGCTTTGATAAAATGTCACTGCAGTTGTAACAATAGCTAGAGTTCCATGTTGAGCTTTAGAGATGGTCTCTTTGTATTTTTACAACTTAATACATTCTGCCTTGAAATTTCAGTTACAAGTGTCAGACAAAGCATTATCACATGTTATGGATATGGGATTTAAAGAAAAAGAAGCAAAGAGAGCACTGCGCATGTGCTATCAAGATGTATGTAAGGCTGTTGATTTTCTTTTTGAGGAAAGAACAAGGAGAGAGAGAAAACGGGAAGAGGATGTTCGACGCCAGGAAGAAATCATGTATGTGTTCTGAGCAAAACTAACTTTTATCTTCTCCCAGTAGTTTATGAGCTGTCTATAATATTGATAACCTGAATAAAATTATCAGGGAGCAAAAACTATATGGAATGACGCCCTCCAAGAAAGCTGTCAATCTTCAGATATTGGAAAAACTGGCATCTATTGGGTAAGTAGACTAAACCAATGTTCTTTTCTTCCAAAATGTCAAAAGAAAAACATATCAATGAATGATAACTTTGTTTTTTTATGTGGCAACTAATGTGAAATTGTTGTGGAATTACTTCTCATCAAGACTTTTCATGAATGGTTGCATCAGAAACATAGTGTAATATGGATCCTGATGATCAAATTTGGACTATAATTTAATCTTATAGGCTTCTTAACCTGTCATTTGGTACCTATAATGAGATCTCGATCGAACTTTCTTAGAAGTCTTTTAGGAGTTGAAAGTGGACTGAGTTCAGACTAAACTCAACTGTCTTCTAAGGGAGTGGGCAAGGTTGTCTCAAGCAAGTATATTTTAGAATAATGCAAATCTTGGTCTATCCAGTATCCATGATATGTGACAAGTATCAGGCTTTTCTGGGTGGGAACCTAACCTGGAAAACTTCAGTACTCTTGATATTCATTGATATGTTTTACAACTCACTTTGGAAGTTTAATAATGCTTTCAGCTAATCACCTTTCAAATTGTCTCATTTTATGTTTTGTGTTGATGAGCTCTTCGATTAAATTGTTTGGCAAGCCACAGCTTCGTGATTTGTGACCACTTTTATCAATTAAGTTGCAATATATGAGCACGGTTTGAGAATTGTTGTAGTCATTTTTCAAGCTAAGACTAGCCTGGTTTTCTAGGTATGAGAAGGAATTAGCTGCCGAAGCCCTGAGAAGAAATGAGAATGACATACAGAAAGCATTGGATGATTTGATAAGCCCAGAGTCTAACTCTGTTATACAGGTGTCTTGGATTTTGATTTTTATCCAGTGTGAGTTGGTTTTTTCGTTAAAACTTTTACCTTGCTAAATTCGTGCCATTCGCAATTCTGCAGAATGCTGTCGAGTCAAGGAAAAGGAAACGACAACGTAAAAGAGCAGATGATGCAGTTGATAAGCTGGTTTGCATGGGTTTTGATAAAACAAGAGGTACCTATTTATGATCGAAACCATTATAGGGTGATTATTATAAATCCAGAAACCCTTTATAGGACTCATGTATCTAAGTACAACAACAAACTTGTTGATCTTATGTTATTAAATATGAAGAAACTTTGATCAAACTAGTAACGCTATAAAATCCTTACTGTGTGCAGCCGAGGCAGCTTTTGAGGCTGGTGGCTCCTTAGAGCAAGCAGTAATTATACTATCAGATCCTGGGACAAACAGTACGGTCGATGGGCAACCTGACTCGGTCAATGCAGCCTTGACTTCTGAGGGTGCAAGTTCCTCCCTTTCAAACATTGCAGAAAACCAAGAAATGTTGGACACCTTGGATGATGAGGGAGGGCCTTCTATCGGTGAAGAAGACCGAGACTTGGAGATGGAAGATGAACTTGCTAGAGAACTCAACGGCGCTACTGCCGTATCGGATTACGACATGGATGTAACAAAAGAAGGAGAAGCCATAACAGAGTATTTGGGTTTGTTGGACTCAATGATGAATTCTGAGAATGCCTAAGTAATACTAACAGGGTTTTTATGGTGTAAATAATATTGCTTTTGAATTCTGAATCTGGTTAGCTTCCTGTAAGTATCAGAGGTATTGTTTAGAGACAAAACTATCCTTGACAACAAGGTTTAAGACGAATTATCCGACAACTCGAATAACATAGACTATCCAACACATATTATAAGGGTTGGATTAGGTTATTTTAAAATTTGAGTTGGGTTGTATTAGGTTTTTTTTTAGTTGGGTTGTGTCTGGGATTGAGATTTGAAAAACTCAATTCAACTCAACTTGAATTAATGTTATTATATATATTTAGTTTTATAGTATCATGCTTGGTACACTTTTTTTAATAATACTAGATGTTTTAAGTATTATA

mRNA sequence

TCAATAGAATATTTTACTTTAAAGTTAATAAAATAACTATTATGCAATCAATTAGAAGATATGAATATATTTGAACATTTTCAAGCAATTATGTTAGGAATGTTACTCATAATTAAAAAAAAAAAATCAAATGAGCAATAAAACAAAATTTATGGGAAGTAAGATTGAACAAATGTAGAACTAAATCAAAGATAAAATTCTGATCTTTAAAAGGTGGATAGGCTAATGCTCAAGGCCCAAGCCGAACACGAAAACTCGCGTAAACAAACTTGAAAGCGGAGAAATTTGATCCAAATTGTCCCGAAATTCCAAGACTTGACCAACAAGGAAACCTCATCGTACCTTTTAAAGCCTCCTTTCATTAATTCCAATCATACCCACCTCGATTTTCTCCATCTTCTCTCATCTGAAGGATCAAAAGACCACTCAGTGACTTTCCCACCCTCCCTTTTTACAGTCCCAATTTCTATTTTGGTTCAGTTTTCATGGCCAAACTCAAGATTTCAGGTCCATGGACTGGTGTGTTGGATGTAGAACTCGATAATTGGACTGTTGTCATGTTGAGGGAAGAAATTGCAAGACGATCGAACTGTGGTGTAGAGTCTATCAATTTAATTTCTGGTGGCAGAATCTTGAAAGATGGTGATGGAAGCGAGAAGCTGGTTCATTTGGGTCTTAAGAACAACTCTAGGATTCTTGCGAGACGGGTTGCTGCTGAAGAGGGGAAGGCACTCAAGAATGAATTGATGGCTGAGGAAGAACGCTCTTCCAGGCTTGCTCGAGTCAAGGCAGCTGCCACTGCTCTTGCTGAGAGGCATGCTAATGGTTTTCTCCCAGTTGAAGATTTCAATATGGAACTGGAGGATCAAAGTGGCCAGAAAGTTAATTTTGGGTCTGAAACTGACCAAAAGGCAATCATGATGGGTCTTATGCTTCATGCAAACGCAAAGCAGCTGATAAGAAAAGGAAACTACAAAGATGCATTAGAAGTACTCGTCATGGGAGAGGAGGCTTTTACTCTATGCAATCCAAAACTTATTGAGTTTGTTGACAACGTCTCAATACTTCAAATAGACATGGTTTGGTGCTATTTCATGCTAAAAGACATCAAGTGGCTCTCAGATGCCGGATTACGCCTTGCAAAGGCTAGAGAAGGAATCGAACGTGCCCATGGGAAGGACTCTTCCCGCGTTCGAATCCTTCAAGCTGGTTGCCATCCTGAGCTTGCCTTACATTTGAGACTGGAGCTGCTGGAGGGGGTGGCTGCATATCACAGCTGTAGGTTTGATAAGTCTCGGGAAGCAATCAACTCAGCACGAGCAAAATATTTTCAGTTACAAGTGTCAGACAAAGCATTATCACATGTTATGGATATGGGATTTAAAGAAAAAGAAGCAAAGAGAGCACTGCGCATGTGCTATCAAGATGTATGTAAGGCTGTTGATTTTCTTTTTGAGGAAAGAACAAGGAGAGAGAGAAAACGGGAAGAGGATGTTCGACGCCAGGAAGAAATCATGGAGCAAAAACTATATGGAATGACGCCCTCCAAGAAAGCTGTCAATCTTCAGATATTGGAAAAACTGGCATCTATTGGGTATGAGAAGGAATTAGCTGCCGAAGCCCTGAGAAGAAATGAGAATGACATACAGAAAGCATTGGATGATTTGATAAGCCCAGAGTCTAACTCTGTTATACAGAATGCTGTCGAGTCAAGGAAAAGGAAACGACAACGTAAAAGAGCAGATGATGCAGTTGATAAGCTGGTTTGCATGGGTTTTGATAAAACAAGAGCCGAGGCAGCTTTTGAGGCTGGTGGCTCCTTAGAGCAAGCAGTAATTATACTATCAGATCCTGGGACAAACAGTACGGTCGATGGGCAACCTGACTCGGTCAATGCAGCCTTGACTTCTGAGGGTGCAAGTTCCTCCCTTTCAAACATTGCAGAAAACCAAGAAATGTTGGACACCTTGGATGATGAGGGAGGGCCTTCTATCGGTGAAGAAGACCGAGACTTGGAGATGGAAGATGAACTTGCTAGAGAACTCAACGGCGCTACTGCCGTATCGGATTACGACATGGATGTAACAAAAGAAGGAGAAGCCATAACAGAGTATTTGGGTTTGTTGGACTCAATGATGAATTCTGAGAATGCCTAAGTAATACTAACAGGGTTTTTATGGTGTAAATAATATTGCTTTTGAATTCTGAATCTGGTTAGCTTCCTGTAAGTATCAGAGGTATTGTTTAGAGACAAAACTATCCTTGACAACAAGGTTTAAGACGAATTATCCGACAACTCGAATAACATAGACTATCCAACACATATTATAAGGGTTGGATTAGGTTATTTTAAAATTTGAGTTGGGTTGTATTAGGTTTTTTTTTAGTTGGGTTGTGTCTGGGATTGAGATTTGAAAAACTCAATTCAACTCAACTTGAATTAATGTTATTATATATATTTAGTTTTATAGTATCATGCTTGGTACACTTTTTTTAATAATACTAGATGTTTTAAGTATTATA

Coding sequence (CDS)

ATGGCCAAACTCAAGATTTCAGGTCCATGGACTGGTGTGTTGGATGTAGAACTCGATAATTGGACTGTTGTCATGTTGAGGGAAGAAATTGCAAGACGATCGAACTGTGGTGTAGAGTCTATCAATTTAATTTCTGGTGGCAGAATCTTGAAAGATGGTGATGGAAGCGAGAAGCTGGTTCATTTGGGTCTTAAGAACAACTCTAGGATTCTTGCGAGACGGGTTGCTGCTGAAGAGGGGAAGGCACTCAAGAATGAATTGATGGCTGAGGAAGAACGCTCTTCCAGGCTTGCTCGAGTCAAGGCAGCTGCCACTGCTCTTGCTGAGAGGCATGCTAATGGTTTTCTCCCAGTTGAAGATTTCAATATGGAACTGGAGGATCAAAGTGGCCAGAAAGTTAATTTTGGGTCTGAAACTGACCAAAAGGCAATCATGATGGGTCTTATGCTTCATGCAAACGCAAAGCAGCTGATAAGAAAAGGAAACTACAAAGATGCATTAGAAGTACTCGTCATGGGAGAGGAGGCTTTTACTCTATGCAATCCAAAACTTATTGAGTTTGTTGACAACGTCTCAATACTTCAAATAGACATGGTTTGGTGCTATTTCATGCTAAAAGACATCAAGTGGCTCTCAGATGCCGGATTACGCCTTGCAAAGGCTAGAGAAGGAATCGAACGTGCCCATGGGAAGGACTCTTCCCGCGTTCGAATCCTTCAAGCTGGTTGCCATCCTGAGCTTGCCTTACATTTGAGACTGGAGCTGCTGGAGGGGGTGGCTGCATATCACAGCTGTAGGTTTGATAAGTCTCGGGAAGCAATCAACTCAGCACGAGCAAAATATTTTCAGTTACAAGTGTCAGACAAAGCATTATCACATGTTATGGATATGGGATTTAAAGAAAAAGAAGCAAAGAGAGCACTGCGCATGTGCTATCAAGATGTATGTAAGGCTGTTGATTTTCTTTTTGAGGAAAGAACAAGGAGAGAGAGAAAACGGGAAGAGGATGTTCGACGCCAGGAAGAAATCATGGAGCAAAAACTATATGGAATGACGCCCTCCAAGAAAGCTGTCAATCTTCAGATATTGGAAAAACTGGCATCTATTGGGTATGAGAAGGAATTAGCTGCCGAAGCCCTGAGAAGAAATGAGAATGACATACAGAAAGCATTGGATGATTTGATAAGCCCAGAGTCTAACTCTGTTATACAGAATGCTGTCGAGTCAAGGAAAAGGAAACGACAACGTAAAAGAGCAGATGATGCAGTTGATAAGCTGGTTTGCATGGGTTTTGATAAAACAAGAGCCGAGGCAGCTTTTGAGGCTGGTGGCTCCTTAGAGCAAGCAGTAATTATACTATCAGATCCTGGGACAAACAGTACGGTCGATGGGCAACCTGACTCGGTCAATGCAGCCTTGACTTCTGAGGGTGCAAGTTCCTCCCTTTCAAACATTGCAGAAAACCAAGAAATGTTGGACACCTTGGATGATGAGGGAGGGCCTTCTATCGGTGAAGAAGACCGAGACTTGGAGATGGAAGATGAACTTGCTAGAGAACTCAACGGCGCTACTGCCGTATCGGATTACGACATGGATGTAACAAAAGAAGGAGAAGCCATAACAGAGTATTTGGGTTTGTTGGACTCAATGATGAATTCTGAGAATGCCTAA

Protein sequence

MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLVHLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVEDFNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLCNPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQAGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFKEKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNLQILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRADDAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASSSLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAITEYLGLLDSMMNSENA*
Homology
BLAST of CsGy2G002280 vs. ExPASy Swiss-Prot
Match: P54729 (NEDD8 ultimate buster 1 OS=Mus musculus OX=10090 GN=Nub1 PE=1 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 4.0e-35
Identity = 143/541 (26.43%), Postives = 257/541 (47.50%), Query Frame = 0

Query: 26  LREEIARRSNCGVESINLISGGRILKDGDGSEKLVHLGLKNNSRILARRVAAEEGKALKN 85
           LR +IA         I ++   + L+ G   + L   G+ +N + +   +   E    KN
Sbjct: 113 LRCQIAETFGFQENYIKIVINKKQLQLG---KSLEEQGVTHNVKAMVLELKQSEEDVRKN 172

Query: 86  ELMAEEERSS------RLARVKAAATALAERHANGFLPVEDFNMELEDQSGQKVNFGSET 145
             + EEE++       R+ R K     LAER      P     +++ +Q+G+ +      
Sbjct: 173 LQLEEEEQNEAELKERRIQRTKRGLEILAERAEMVVDPETMPYLDIANQTGRSLRI-PPA 232

Query: 146 DQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLCNPKLIEFVDNVSILQIDMV 205
           ++KA+M+ +  H   +  +++  Y  AL  L+  +  F  C  +L++ VDN ++LQ+D+V
Sbjct: 233 ERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADRYFCECK-ELLDTVDNYAVLQLDIV 292

Query: 206 WCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQAGCHPELALHLRLELLEGV 265
           WCYF L+ ++ L DA  +L  A++  +  +G++  R+  ++  C  E  L LRL LL+G+
Sbjct: 293 WCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQGI 352

Query: 266 AAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFKEKEAKRALRMCYQDVCKAV 325
             YHS   +++RE +N AR  + +L +    + +++ +GF  +EA+  LR C  +V  A 
Sbjct: 353 QNYHSGNGEEAREYLNKARQLFKELYIDPSKVHNLLQLGFTAQEARLGLRACDGNVDHAA 412

Query: 326 DFLFEERTRRERKREE--DVRRQEEIMEQKLYGMTPSKKAVNLQILEKLASIGYEKELAA 385
                  T    +REE   +R++E+            K+   L+ +  L  +GY  + A 
Sbjct: 413 -------THISNRREELAQIRKEEK-----------EKRRRRLENVNTLRGMGYSTQAAK 472

Query: 386 EALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRADDAVDKLVCMGFDKTRAE 445
           +AL +   ++  AL  L+S      +Q+A +     RQ   + +++++LV MGFD   AE
Sbjct: 473 QALHQARGNLDDALKVLLSNPHMWWLQDA-DPENNSRQASPSQESINQLVYMGFDTVVAE 532

Query: 446 AAFEA-GGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASSSLSNIAENQEMLDTLD 505
           AA    GG+++ A   L+  G +   D Q    +++ T   + S                
Sbjct: 533 AALRVFGGNVQLAAQTLAHHGGSLPPDLQFSGEDSSPTPSTSPS---------------- 592

Query: 506 DEGGPSIGEEDRDLEME--DELARELNGATAVSDYDMDVTKEGE--AITEYLGLLDSMMN 554
           D  G S    D D+E E  +E+  ++       DY +D T E E   I EYL  ++S+ +
Sbjct: 593 DSAGTSSASTDEDMETEAVNEILEDI--PEHEEDY-LDSTLEDEEVIIAEYLSYVESISS 610

BLAST of CsGy2G002280 vs. ExPASy Swiss-Prot
Match: Q9Y5A7 (NEDD8 ultimate buster 1 OS=Homo sapiens OX=9606 GN=NUB1 PE=1 SV=2)

HSP 1 Score: 140.2 bits (352), Expect = 7.1e-32
Identity = 132/506 (26.09%), Postives = 243/506 (48.02%), Query Frame = 0

Query: 26  LREEIARRSNCGVESINLISGGRILKDGDGSEKLVHLGLKNNSRILARRVAAEEGKALKN 85
           LR +IA         I ++   + L+ G   + L   G+ +N + +   +   E  A KN
Sbjct: 112 LRSKIAETFGLQENYIKIVINKKQLQLG---KTLEEQGVAHNVKAMVLELKQSEEDARKN 171

Query: 86  ELMAEEE------RSSRLARVKAAATALAERHANGFL-PVEDFNMELEDQSGQKVNFGSE 145
             + EEE      +  ++ R K     LA+R A   + P     +++ +Q+G+ +     
Sbjct: 172 FQLEEEEQNEAKLKEKQIQRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRI-PP 231

Query: 146 TDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLCNPKLIEFVDNVSILQIDM 205
           +++KA+M+ +  H   +  +++  Y  AL  L+  ++ F  C  +L++ VDN ++LQ+D+
Sbjct: 232 SERKALMLAMGYHEKGRAFLKRKEYGIALPCLLDADKYFCECCRELLDTVDNYAVLQLDI 291

Query: 206 VWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQAGCHPELALHLRLELLEG 265
           VWCYF L+ ++ L DA  +L  A++  +  +G++  R+  ++  C  E  L LRL LL+G
Sbjct: 292 VWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLVHIKGNCGKEKVLFLRLYLLQG 351

Query: 266 VAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFKEKEAKRALRMCYQDVCKA 325
           +  YHS    ++ E +N AR  + +L +    + +++ +GF  +EA+  LR C  +V  A
Sbjct: 352 IRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLGLRACDGNVDHA 411

Query: 326 VDFLFEERTRRERKREE--DVRRQEEIMEQKLYGMTPSKKAVNLQILEKLASIGYEKELA 385
                   T    +REE   +R++E+            KK   L+ +  L  +GY    A
Sbjct: 412 A-------THITNRREELAQIRKEEK-----------EKKRRRLENIRFLKGMGYSTHAA 471

Query: 386 AEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRADDAVDKLVCMGFDKTRA 445
            + L     ++ +AL  L+S      + N        RQ   + + +D+LV MGFD   A
Sbjct: 472 QQVLHAASGNLDEALKILLSNPQMWWL-NDSNPETDNRQESPSQENIDRLVYMGFDALVA 531

Query: 446 EAAFEA-GGSLEQAVIILSDPGTNSTVD---GQPDSVNAALTSEGASSSLSNIAENQEM- 505
           EAA     G+++ A   L+  G +   +      DS++   TS   S+  S+ + +++M 
Sbjct: 532 EAALRVFRGNVQLAAQTLAHNGGSLPPELPLSPEDSLSPPATSPSDSAGTSSASTDEDME 591

Query: 506 LDTLDD--EGGPSIGEEDRDLEMEDE 516
            + +++  E  P   E+  D  +EDE
Sbjct: 592 TEAVNEILEDIPEHEEDYLDSTLEDE 594

BLAST of CsGy2G002280 vs. NCBI nr
Match: XP_004139279.1 (NEDD8 ultimate buster 1 [Cucumis sativus] >KGN60729.1 hypothetical protein Csa_019386 [Cucumis sativus])

HSP 1 Score: 1061 bits (2744), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
           HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD
Sbjct: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
           DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS
Sbjct: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of CsGy2G002280 vs. NCBI nr
Match: XP_008457303.1 (PREDICTED: NEDD8 ultimate buster 1 [Cucumis melo])

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 528/556 (94.96%), Postives = 542/556 (97.48%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELDNWTV MLREEIARRSNC VESINLISGGRILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVRMLREEIARRSNCSVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
           HLGLKNNSRILARRV+ EEGKALKNELMAEEERS+RLARVKAAATALAERHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRMLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+REAINSARAKYFQLQVSD+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMC+QDVCKAVDFLFEERT+RERKREED+RRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCHQDVCKAVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           QILEKL SIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNA+ESRKRKRQRKR D
Sbjct: 361 QILEKLTSIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAIESRKRKRQRKRVD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
            AVDKLV MGFDKTRAEAAFEAGGSLEQA+IILS+PGTN+ V GQPDSVNA L SEGASS
Sbjct: 421 GAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGTNTAVHGQPDSVNAVLASEGASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLDDEGG SIGEEDRD  MEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGSSIGEEDRDSVMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of CsGy2G002280 vs. NCBI nr
Match: XP_038890750.1 (NEDD8 ultimate buster 1 [Benincasa hispida])

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 498/556 (89.57%), Postives = 533/556 (95.86%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELD WTV MLREEIARRSNCGVESINLISGG+ILKDGDGSEKL+
Sbjct: 1   MAKLKISGPWTGVLDVELDIWTVPMLREEIARRSNCGVESINLISGGKILKDGDGSEKLI 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
           HLGLKNNSRILARRV+ EEGKALK+ELMAEEERS+RLARVKAAATALAERHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKDELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIK+LSDAG+RLA+AREGIER+HGKDSSRVRILQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKFLSDAGIRLARAREGIERSHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+REAINSARAKYFQLQVSD+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           E+EAKRALRMC+QDVCKAVDFLFEER +RER+REED+RRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EREAKRALRMCHQDVCKAVDFLFEERAKRERRREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           QIL++LASIGYEKELAAEALRRNEND+QKALDDLISPESNSVIQNA+ESRKRKRQ K AD
Sbjct: 361 QILKELASIGYEKELAAEALRRNENDLQKALDDLISPESNSVIQNAIESRKRKRQHKTAD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
           DAVD LV MGFD+T+A AAFEAGGSLEQA+IILS+  TN+TV+GQPDSV AAL SEG SS
Sbjct: 421 DAVDNLVRMGFDRTKAGAAFEAGGSLEQALIILSELETNTTVNGQPDSVTAALASEGTSS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQ MLDT DD  GP  GEEDRDLEMEDELAREL+GA+AVSDYD+DVTKEGEAI
Sbjct: 481 SLSNIAENQ-MLDTFDDGEGPE-GEEDRDLEMEDELARELDGASAVSDYDLDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYL LL++ MNSE A
Sbjct: 541 TEYLNLLETTMNSEKA 554

BLAST of CsGy2G002280 vs. NCBI nr
Match: XP_023537732.1 (NEDD8 ultimate buster 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 903 bits (2334), Expect = 0.0
Identity = 473/556 (85.07%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELDNWTV+MLREEIARRSNCGVESINLI GGRILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
            LG+KNNSRILARRV+ EEGK+L  ELMAEEERS+RLARVKAAATALA+RHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPK+IEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDK+REAINSA+ KY QLQV D+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMC+QDVCKAVDFLFEERT+RE+KREED+ RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNENDIQKALDDLI+PE+NSVIQNAVESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDIQKALDDLINPETNSVIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQAV +LS P T STVDGQPDSV     SE ASS
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSGSEDASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  D+  GPS  EE+RD EME ELAREL+GA+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYL LLDS+MNSE A
Sbjct: 541 TEYLALLDSLMNSEKA 556

BLAST of CsGy2G002280 vs. NCBI nr
Match: XP_022938166.1 (NEDD8 ultimate buster 1 [Cucurbita moschata])

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 472/556 (84.89%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELDNWTV+MLREEIARRSNCGVESINLI GGRILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
            LG+KNNSRILARRV+ EEGK+L  ELMAEEERS+RLARVKAAATALA+RHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDK+REAINSA+ KY QLQV D+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMC+QDVCKAVDFLFEERT+R++KREED+ RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNEND+QKALDDLI+PE+NSVIQNAVESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQAV +LS P T STVDGQPDSV     SE ASS
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSASEYASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  D+  GPS  EE+RD EME ELAREL+GA+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYL LLDS+MNSE A
Sbjct: 541 TEYLALLDSLMNSEKA 556

BLAST of CsGy2G002280 vs. ExPASy TrEMBL
Match: A0A0A0LI64 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008690 PE=4 SV=1)

HSP 1 Score: 1061 bits (2744), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
           HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD
Sbjct: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
           DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS
Sbjct: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of CsGy2G002280 vs. ExPASy TrEMBL
Match: A0A1S3C5V6 (NEDD8 ultimate buster 1 OS=Cucumis melo OX=3656 GN=LOC103497022 PE=4 SV=1)

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 528/556 (94.96%), Postives = 542/556 (97.48%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPWTGVLDVELDNWTV MLREEIARRSNC VESINLISGGRILKDGDGSEKLV
Sbjct: 1   MAKLKISGPWTGVLDVELDNWTVRMLREEIARRSNCSVESINLISGGRILKDGDGSEKLV 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
           HLGLKNNSRILARRV+ EEGKALKNELMAEEERS+RLARVKAAATALAERHANGFLPVED
Sbjct: 61  HLGLKNNSRILARRVSTEEGKALKNELMAEEERSTRLARVKAAATALAERHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC
Sbjct: 121 FNIELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRMLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+REAINSARAKYFQLQVSD+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCKFDKAREAINSARAKYFQLQVSDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           EKEAKRALRMC+QDVCKAVDFLFEERT+RERKREED+RRQEEIMEQKLYGMTPSKKAVNL
Sbjct: 301 EKEAKRALRMCHQDVCKAVDFLFEERTKRERKREEDIRRQEEIMEQKLYGMTPSKKAVNL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           QILEKL SIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNA+ESRKRKRQRKR D
Sbjct: 361 QILEKLTSIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAIESRKRKRQRKRVD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
            AVDKLV MGFDKTRAEAAFEAGGSLEQA+IILS+PGTN+ V GQPDSVNA L SEGASS
Sbjct: 421 GAVDKLVRMGFDKTRAEAAFEAGGSLEQALIILSEPGTNTAVHGQPDSVNAVLASEGASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           SLSNIAENQEMLDTLDDEGG SIGEEDRD  MEDELARELNGATAVSDYDMDVTKEGEAI
Sbjct: 481 SLSNIAENQEMLDTLDDEGGSSIGEEDRDSVMEDELARELNGATAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYLGLLDSMMNSENA
Sbjct: 541 TEYLGLLDSMMNSENA 556

BLAST of CsGy2G002280 vs. ExPASy TrEMBL
Match: A0A6J1FCD2 (NEDD8 ultimate buster 1 OS=Cucurbita moschata OX=3662 GN=LOC111444333 PE=4 SV=1)

HSP 1 Score: 902 bits (2332), Expect = 0.0
Identity = 472/556 (84.89%), Postives = 512/556 (92.09%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELDNWTV+MLREEIARRSNCGVESINLI GGRILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
            LG+KNNSRILARRV+ EEGK+L  ELMAEEERS+RLARVKAAATALA+RHANGFLPVED
Sbjct: 61  DLGVKNNSRILARRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDK+REAINSA+ KY QLQV D+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMC+QDVCKAVDFLFEERT+R++KREED+ RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKRDKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNEND+QKALDDLI+PE+NSVIQNAVESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDLQKALDDLINPETNSVIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQAV +LS P T STVDGQPDSV     SE ASS
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTVSASEYASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  D+  GPS  EE+RD EME ELAREL+GA+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDGASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYL LLDS+MNSE A
Sbjct: 541 TEYLALLDSLMNSEKA 556

BLAST of CsGy2G002280 vs. ExPASy TrEMBL
Match: A0A6J1HMA3 (NEDD8 ultimate buster 1 OS=Cucurbita maxima OX=3661 GN=LOC111465491 PE=4 SV=1)

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 470/556 (84.53%), Postives = 509/556 (91.55%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPW+GVLDVELDNWTV+MLREEIARRSNCGVESINLI GGRILKDGDG EKL+
Sbjct: 1   MAKLKISGPWSGVLDVELDNWTVIMLREEIARRSNCGVESINLICGGRILKDGDGGEKLL 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
            LG+KNNSRILA RV+ EEGK+L  ELMAEEERS+RLARVKAAATALA+RHANGFLPVED
Sbjct: 61  DLGVKNNSRILAMRVSTEEGKSLNVELMAEEERSNRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKVN GSETDQ+AIMMGLMLHANAKQLIRKGNYK+ALEVL+MGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVNLGSETDQRAIMMGLMLHANAKQLIRKGNYKEALEVLIMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPK+IEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKVIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC FDK+REAINSA+ KY QLQV D+ALSHVMDMGFK
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCMFDKAREAINSAQMKYSQLQVPDEALSHVMDMGFK 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           E EAKRALRMC+QDVCKAVDFLFEERT+RE+KREED+ RQ+EI EQK YGMTPSKKAV+L
Sbjct: 301 EGEAKRALRMCHQDVCKAVDFLFEERTKREKKREEDIERQKEIKEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRAD 420
           Q L++LASIGYEK LAAEALRRNENDIQKALDDLI+PE+NS IQNAVESRKRKRQR+  D
Sbjct: 361 QRLKELASIGYEKNLAAEALRRNENDIQKALDDLINPETNSFIQNAVESRKRKRQRQTTD 420

Query: 421 DAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGASS 480
            AVDKLV MGFD+TRA AAF+AGGSLEQAV +LS P T STVDGQPDSV  A  SE ASS
Sbjct: 421 AAVDKLVLMGFDRTRAIAAFQAGGSLEQAVNLLSLPDTKSTVDGQPDSVPTASASEDASS 480

Query: 481 SLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEAI 540
           S+SNI E Q+MLD  D+  GPS  EE+RD EME ELAREL+ A+AVSDYDMDVTKEGEAI
Sbjct: 481 SISNIEEIQDMLDNFDNGEGPSTVEENRDSEMESELARELDRASAVSDYDMDVTKEGEAI 540

Query: 541 TEYLGLLDSMMNSENA 556
           TEYL LLDS+MNS  A
Sbjct: 541 TEYLALLDSLMNSAKA 556

BLAST of CsGy2G002280 vs. ExPASy TrEMBL
Match: A0A6J1CMT5 (NEDD8 ultimate buster 1 OS=Momordica charantia OX=3673 GN=LOC111013048 PE=4 SV=1)

HSP 1 Score: 853 bits (2203), Expect = 5.01e-308
Identity = 444/557 (79.71%), Postives = 502/557 (90.13%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILKDGDGSEKLV 60
           MAKLKISGPW GVL+VELDNWTV MLREE+ARRSNCG+ESINLI GGR+LKDGDGSEKL+
Sbjct: 1   MAKLKISGPWAGVLEVELDNWTVPMLREEVARRSNCGIESINLICGGRVLKDGDGSEKLI 60

Query: 61  HLGLKNNSRILARRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPVED 120
           +LG+KNNSRILARRV+ EEGK+LK+ELMAEEERS+RLARVKAAATALA+RHANGFLPVED
Sbjct: 61  NLGVKNNSRILARRVSTEEGKSLKDELMAEEERSTRLARVKAAATALAKRHANGFLPVED 120

Query: 121 FNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFTLC 180
           FN+ELEDQSGQKV+ GSETDQ+AIMMGLMLHANAKQLI+KGNYK+ALEVLVMGEEAF+LC
Sbjct: 121 FNIELEDQSGQKVHMGSETDQRAIMMGLMLHANAKQLIKKGNYKEALEVLVMGEEAFSLC 180

Query: 181 NPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRILQ 240
           NPK+IEFVDNV ILQIDMVWCYFMLKDIKWLS+AGLRLAKAREGIERAHGKDSSRVR+LQ
Sbjct: 181 NPKVIEFVDNVPILQIDMVWCYFMLKDIKWLSEAGLRLAKAREGIERAHGKDSSRVRLLQ 240

Query: 241 AGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMGFK 300
           AGCHPELALHLRLELLEGVAAYHSC+FDK+++AINSA+AKYFQLQV D+ALS VM MGF+
Sbjct: 241 AGCHPELALHLRLELLEGVAAYHSCQFDKAQKAINSAQAKYFQLQVPDEALSLVMSMGFE 300

Query: 301 EKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAVNL 360
           E+EAK ALRMC+QDV  AVDFL  ER +RE+KREED++R++EI EQK YGMTPSKKAV+L
Sbjct: 301 EREAKSALRMCHQDVANAVDFLVGERAKREKKREEDIQREKEIEEQKRYGMTPSKKAVDL 360

Query: 361 QILEKLASI-GYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKRA 420
           Q L++L SI GYE+ELAAEALRRNEND QKALDDL +PE+NS+IQN +ESRKRKRQR+ A
Sbjct: 361 QRLKELVSIIGYERELAAEALRRNENDFQKALDDLTNPETNSLIQNTIESRKRKRQRQTA 420

Query: 421 DDAVDKLVCMGFDKTRAEAAFEAGGSLEQAVIILSDPGTNSTVDGQPDSVNAALTSEGAS 480
           D  VD LV MGFD+T+A AA +A GSLEQA+ +LS P TN+TVDGQPDS  AA+  EGA+
Sbjct: 421 DSTVDTLVRMGFDRTKAVAASQAAGSLEQALHLLSLPETNTTVDGQPDSAFAAVAFEGAN 480

Query: 481 SSLSNIAENQEMLDTLDDEGGPSIGEEDRDLEMEDELARELNGATAVSDYDMDVTKEGEA 540
           SSLSNI   ++MLD  DD  GPS  EE+RD EME +LA ELNGATAVSDYDMDVTKEGEA
Sbjct: 481 SSLSNIDGKEDMLDNFDDGEGPSTAEEERDSEMESKLAMELNGATAVSDYDMDVTKEGEA 540

Query: 541 ITEYLGLLDSMMNSENA 556
           ITEYL  LDS+ N E A
Sbjct: 541 ITEYLVFLDSVENLEKA 557

BLAST of CsGy2G002280 vs. TAIR 10
Match: AT2G12550.1 (ubiquitin-associated (UBA)/TS-N domain-containing protein )

HSP 1 Score: 567.8 bits (1462), Expect = 9.8e-162
Identity = 312/565 (55.22%), Postives = 415/565 (73.45%), Query Frame = 0

Query: 1   MAKLKISGPWTGVLDVELDNWTVVMLREEIARRSNCGVESINLISGGRILK-DGDGSEKL 60
           MAKLK++G W G+L+VEL+NWT+ MLR+E+A+RS    ESI LI  G+ILK DGD +  L
Sbjct: 1   MAKLKVAGTWGGILEVELENWTLNMLRDEVAKRSGLDPESIKLIFAGKILKDDGDATLTL 60

Query: 61  VHLGLKNNSRILA-RRVAAEEGKALKNELMAEEERSSRLARVKAAATALAERHANGFLPV 120
             LG+K NS+IL+ R+ A EEGK+    +MAEEER+ RL+RVKAAATAL++RHA+G LP+
Sbjct: 61  SQLGIKENSKILSCRQAAPEEGKS----IMAEEERARRLSRVKAAATALSKRHADGSLPI 120

Query: 121 EDFNMELEDQSGQKVNFGSETDQKAIMMGLMLHANAKQLIRKGNYKDALEVLVMGEEAFT 180
           +D+N+ELEDQ G KV FG+ETDQ A MMGLMLH  AK LI    Y DA+EVL M EE+F 
Sbjct: 121 QDYNIELEDQGGHKVQFGTETDQSAAMMGLMLHTKAKSLIEIEMYSDAVEVLAMAEESFL 180

Query: 181 LCNPKLIEFVDNVSILQIDMVWCYFMLKDIKWLSDAGLRLAKAREGIERAHGKDSSRVRI 240
           LC+PK++E VDN+ +++ID+VWCYF+L+DIK LSDAG+RL +AR+G+ERAHGKD SRVR+
Sbjct: 181 LCDPKILELVDNLPMMEIDIVWCYFLLRDIKCLSDAGVRLVRARKGLERAHGKDLSRVRL 240

Query: 241 LQAGCHPELALHLRLELLEGVAAYHSCRFDKSREAINSARAKYFQLQVSDKALSHVMDMG 300
           LQAG  PE+A++LRLELLEGV AYH+ + DK+  A+ SA AK+ QLQ+ D+ LS VM MG
Sbjct: 241 LQAGQSPEMAIYLRLELLEGVVAYHTGQNDKALNALKSAHAKFLQLQIPDETLSLVMGMG 300

Query: 301 FKEKEAKRALRMCYQDVCKAVDFLFEERTRRERKREEDVRRQEEIMEQKLYGMTPSKKAV 360
           F+EK+AKRALR+  QD+  +VDFL EER ++ +KRE+D++RQ EI+EQK YG+TP KKAV
Sbjct: 301 FQEKDAKRALRLNNQDIASSVDFLIEERAKKAQKREQDLQRQSEILEQKKYGVTPMKKAV 360

Query: 361 NLQILEKLASIGYEKELAAEALRRNENDIQKALDDLISPESNSVIQNAVESRKRKRQRKR 420
           ++Q+LE+L SIGY +ELAAE+LRRNENDIQKALD L  P+ NS IQ  +ESRKRKRQ + 
Sbjct: 361 DMQMLERLVSIGYARELAAESLRRNENDIQKALDILTDPKVNSTIQAYIESRKRKRQEQL 420

Query: 421 ADDAVDKLVCMGFDKTRAEAAFEAGGSLEQAV-----IILSDPGTNST--VDGQPDSVNA 480
              +V +LV MGF++ +A +A EAGGS E  +       +++PGT +T  ++    + N 
Sbjct: 421 VGISVAELVSMGFERGKATSALEAGGSREDTIQRLLSASVANPGTTTTSVINATSSTNNV 480

Query: 481 ALTSEGASSSLSNIAE-NQEMLDTLDDEGGPSIG--EEDRDLEMEDELARELNGATAVSD 540
              S G        +E   E  D + +    S    EE+RD E+EDE+A E+    A+S 
Sbjct: 481 GAESSGFGGGAEQDSEMKDETTDDIANRASTSSDGMEEERDSEIEDEIADEIARVDALSA 540

Query: 541 YDMDVTKEGEAITEYLGLLDSMMNS 554
           YD+++ KE EAI EYL +LD+   S
Sbjct: 541 YDINLDKEIEAINEYLAMLDASQES 561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P547294.0e-3526.43NEDD8 ultimate buster 1 OS=Mus musculus OX=10090 GN=Nub1 PE=1 SV=2[more]
Q9Y5A77.1e-3226.09NEDD8 ultimate buster 1 OS=Homo sapiens OX=9606 GN=NUB1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_004139279.10.0100.00NEDD8 ultimate buster 1 [Cucumis sativus] >KGN60729.1 hypothetical protein Csa_0... [more]
XP_008457303.10.094.96PREDICTED: NEDD8 ultimate buster 1 [Cucumis melo][more]
XP_038890750.10.089.57NEDD8 ultimate buster 1 [Benincasa hispida][more]
XP_023537732.10.085.07NEDD8 ultimate buster 1 [Cucurbita pepo subsp. pepo][more]
XP_022938166.10.084.89NEDD8 ultimate buster 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LI640.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008690 PE=4 SV=1[more]
A0A1S3C5V60.094.96NEDD8 ultimate buster 1 OS=Cucumis melo OX=3656 GN=LOC103497022 PE=4 SV=1[more]
A0A6J1FCD20.084.89NEDD8 ultimate buster 1 OS=Cucurbita moschata OX=3662 GN=LOC111444333 PE=4 SV=1[more]
A0A6J1HMA30.084.53NEDD8 ultimate buster 1 OS=Cucurbita maxima OX=3661 GN=LOC111465491 PE=4 SV=1[more]
A0A6J1CMT55.01e-30879.71NEDD8 ultimate buster 1 OS=Momordica charantia OX=3673 GN=LOC111013048 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G12550.19.8e-16255.22ubiquitin-associated (UBA)/TS-N domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 73..93
NoneNo IPR availableGENE3D1.10.8.10coord: 397..458
e-value: 1.5E-5
score: 26.9
coord: 266..328
e-value: 2.5E-10
score: 42.2
NoneNo IPR availableGENE3D1.10.8.10coord: 361..396
e-value: 1.0E-5
score: 27.4
IPR015940Ubiquitin-associated domainSMARTSM00165uba_6coord: 287..324
e-value: 1.1E-4
score: 31.5
coord: 419..455
e-value: 31.0
score: 2.7
coord: 359..396
e-value: 0.028
score: 23.6
IPR015940Ubiquitin-associated domainPFAMPF00627UBAcoord: 289..322
e-value: 1.4E-5
score: 24.9
IPR015940Ubiquitin-associated domainPROSITEPS50030UBAcoord: 357..397
score: 10.932388
IPR015940Ubiquitin-associated domainPROSITEPS50030UBAcoord: 285..325
score: 12.570991
IPR039749NEDD8 ultimate buster 1PANTHERPTHR12948NEDD8 ULTIMATE BUSTER-1 BS4 PROTEINcoord: 20..549
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 22..70
score: 8.938011
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 12..71
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 279..326
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 350..396
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 405..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G002280.2CsGy2G002280.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:2000058 regulation of ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0031593 polyubiquitin modification-dependent protein binding
molecular_function GO:0005515 protein binding