CsGy1G033260 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy1G033260 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.4e-57
Identity = 164/547 (29.98%), Postives = 286/547 (52.29%), Query Frame = 0

Query: 441  VITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVA 500
            + T  + V  +     + N     I+++C+S+ L ECE AV  + +   D+  +  I   
Sbjct: 640  ITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSY 699

Query: 501  LSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKS 560
            LS+P ++ G++E+L AS + E+L   I +L+EL    + + + + + D        LLK+
Sbjct: 700  LSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKN 759

Query: 561  SSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQ 620
                 +A++L+Y  +P   Q+ + E +P ++ V++   +    F +   P +AA  +L+Q
Sbjct: 760  G--LAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQ 819

Query: 621  LLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLA 680
             L G DE     N+  +I+  G+  L++ LER  +E R++ VS++ CC+QA+ SC+N +A
Sbjct: 820  TLMGGDEYSRSLNASSVISANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIA 879

Query: 681  ENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNIL 740
              +  + +LEL  H  N +     +  L +L+ L+RRT   +LL  +K+       M+  
Sbjct: 880  NRIELSPVLEL-FHSGNDSVRGTCVEFLSELVQLNRRTSCNQLLHTIKDE-GAFSTMHTF 939

Query: 741  SVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLA 800
             VYLQ A  E Q  VA+ LLQLD + +    SI+REEA+ T+I AL  +      Q    
Sbjct: 940  LVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDF-SNTQMKAL 999

Query: 801  RALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHL---YDDVVQLYEEEEEVMN 860
             ALL L G  S +G+  TE WLLK+AGF +        + L    +D+++  E+E+  +N
Sbjct: 1000 DALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALN 1059

Query: 861  -WQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLM 920
             WQ + ASVL NH + S+  +L   + S     +AK+CL+  +W++R L+ + +  +  +
Sbjct: 1060 SWQKRIASVLCNHENGSIFKALEECLKS-NSLKMAKSCLVLATWLTRMLYTLPDTGVRDV 1119

Query: 921  APSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVT 980
            A   L+  +IK L+  K++ED +L + SL           + R++       LR L   +
Sbjct: 1120 ARKSLLEEVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYS 1178

Query: 981  WTAEELI 984
              A +++
Sbjct: 1180 TVAADIL 1178

BLAST of CsGy1G033260 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 1.3e-55
Identity = 161/550 (29.27%), Postives = 285/550 (51.82%), Query Frame = 0

Query: 441  VITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVA 500
            + T  + V  +     + N     I+++C+S+ L ECE AV  + +   D+  +  I   
Sbjct: 640  ITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSY 699

Query: 501  LSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKS 560
            LS+P ++ G++E+L AS + E+L   I +L+EL    + + + + + D        LLK+
Sbjct: 700  LSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKN 759

Query: 561  SSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQ 620
                 +A++L+Y  +P   Q+ + E +P ++ V++   +    F +   P +AA  +L+Q
Sbjct: 760  G--LAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQ 819

Query: 621  LLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLA 680
             L G DE     N+  +I+  G+  L++ LER  +E R++ VS++ CC+QA+ SC+N +A
Sbjct: 820  TLMGGDEYSRSLNASSVISANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIA 879

Query: 681  ENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNIL 740
              +  + +LEL  H  N +     +  L +L+ L+RRT   ++L  +K+       M+  
Sbjct: 880  NRIELSPVLEL-FHSGNDSVRGTCVEFLSELVQLNRRTSCNQILHTIKDE-GAFSTMHTF 939

Query: 741  SVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLA 800
             VYLQ A  E Q  VA+ LLQLD + +    SI+REEA+ T+I AL  +      Q    
Sbjct: 940  LVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDF-SNTQMKAL 999

Query: 801  RALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHL---YDDVVQLYEEEEEVMN 860
             ALL L G  S +G+  TE WLLK+AGF +        + L    +D+++  E+E+  +N
Sbjct: 1000 DALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALN 1059

Query: 861  -WQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLM 920
             WQ + ASVL NH + S+  +L   + S     +AK+CL+  +W++  L+ + +  +  +
Sbjct: 1060 SWQKRIASVLCNHENGSIFKALEECLKS-NSLKMAKSCLVLATWLTHMLYTLPDTGVRDV 1119

Query: 921  APSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVT 980
            A   L+  +I  L   K +E+++LA+ +L             R++       LR L   +
Sbjct: 1120 ARKSLLEEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKYS 1179

Query: 981  WTAEELILII 987
              A +++ +I
Sbjct: 1180 VVAVDIMKVI 1181

BLAST of CsGy1G033260 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 5.4e-54
Identity = 159/529 (30.06%), Postives = 276/529 (52.17%), Query Frame = 0

Query: 459  NDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASD 518
            N  +  IT++C+S+ L +CE AV  + + W D+  D  I   LS+P VV G++E+L AS 
Sbjct: 661  NSLTPYITSLCTSENLQDCEQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASL 720

Query: 519  DDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 578
            + E+L   I +L+EL    E + + + + D        LLK+     +A++L+Y  +P  
Sbjct: 721  NREVLRRSIYILSELIFSDERVGETLNSVDSDFDCLAMLLKNG--LAEAALLIYQLRPVF 780

Query: 579  KQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLI 638
             Q+   E +P +++V++   +    F +   P  AA  +L+Q+L G DE     N+  +I
Sbjct: 781  AQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVI 840

Query: 639  ALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNK 698
            +  G+  +++ L++   E R+  +SI+ CC+QA+ SC++ +A  +  + +LEL  H  N 
Sbjct: 841  SANGIPAIVKYLDK--TEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLEL-FHAGND 900

Query: 699  NSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATT 758
            +     +  L +L+ L+RRT   + L  +K+       M+   VYLQ A  E Q  VA+ 
Sbjct: 901  SVRGICVEFLSELVRLNRRTSSNQTLQIIKDE-GAFSTMHTFLVYLQMAPMEHQIAVASL 960

Query: 759  LLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPST 818
            LLQLD + +    SI+REEA+ T+I AL  +      Q     ALL L G  + +G+  T
Sbjct: 961  LLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNN-QMKALDALLFLIGHVTSSGKSYT 1020

Query: 819  ENWLLKLAGFKENSG---DSSHSKHLYDDVVQLYEEEEEVM-NWQLKAASVLFNHGHKSL 878
            E  LLK+AGF +       +    H  +D ++  E+E+  M +WQ + ASVL NH + S+
Sbjct: 1021 EAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVASVLCNHENGSI 1080

Query: 879  LSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKA 938
              +L   + S     +AK+CL+  +W++  LF + +  +  +A   L+  L+  L   K 
Sbjct: 1081 FQALEECLKS-NSLKMAKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKN 1140

Query: 939  VEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELI 984
            +E+++LAS +L +         + R++       LR L   +  A +++
Sbjct: 1141 LEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1181

BLAST of CsGy1G033260 vs. NCBI nr
Match: XP_004145484.2 (putative E3 ubiquitin-protein ligase LIN-2 isoform X2 [Cucumis sativus])

HSP 1 Score: 1962 bits (5084), Expect = 0.0
Identity = 1008/1008 (100.00%), Postives = 1008/1008 (100.00%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
            IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV
Sbjct: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL
Sbjct: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420
            RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS
Sbjct: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480
            DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA
Sbjct: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480

Query: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
            VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII
Sbjct: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540

Query: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660
            QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720
            SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780
            TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV
Sbjct: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780

Query: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840
            TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH
Sbjct: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840

Query: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900
            LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW
Sbjct: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900

Query: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960
            MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL
Sbjct: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960

Query: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE 1008
            FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE
Sbjct: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE 1008

BLAST of CsGy1G033260 vs. NCBI nr
Match: XP_031744408.1 (putative E3 ubiquitin-protein ligase LIN-2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1945 bits (5038), Expect = 0.0
Identity = 1008/1043 (96.64%), Postives = 1008/1043 (96.64%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
            IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV
Sbjct: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL
Sbjct: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQ---------------------------- 420
            RRGNLVAAMARSSHEKEILFSDKHYENSASVQ                            
Sbjct: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQVCNSISIFNIESFGSWLEKSRCTVFIFL 420

Query: 421  -------DEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQNITKEYLESSNVITKN 480
                   DEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQNITKEYLESSNVITKN
Sbjct: 421  RSLLFLQDEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQNITKEYLESSNVITKN 480

Query: 481  SFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPP 540
            SFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPP
Sbjct: 481  SFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPP 540

Query: 541  VVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFL 600
            VVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFL
Sbjct: 541  VVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFL 600

Query: 601  KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGF 660
            KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGF
Sbjct: 601  KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGF 660

Query: 661  DEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNK 720
            DEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNK
Sbjct: 661  DEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNK 720

Query: 721  ASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQ 780
            ASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQ
Sbjct: 721  ASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQ 780

Query: 781  RALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLARALLI 840
            RALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLARALLI
Sbjct: 781  RALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKAQDNLARALLI 840

Query: 841  LGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHLYDDVVQLYEEEEEVMNWQLKAASV 900
            LGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHLYDDVVQLYEEEEEVMNWQLKAASV
Sbjct: 841  LGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHLYDDVVQLYEEEEEVMNWQLKAASV 900

Query: 901  LFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPL 960
            LFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPL
Sbjct: 901  LFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPL 960

Query: 961  IKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELILI 1008
            IKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELILI
Sbjct: 961  IKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELILI 1020

BLAST of CsGy1G033260 vs. NCBI nr
Match: XP_038894766.1 (putative E3 ubiquitin-protein ligase LIN isoform X1 [Benincasa hispida])

HSP 1 Score: 1779 bits (4609), Expect = 0.0
Identity = 914/1007 (90.76%), Postives = 964/1007 (95.73%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEGSN+P+TRK  R KGRSR APDDSVTLPIYICHDKK IDSSKKK +
Sbjct: 1    MASLQELLTREGFEGSNFPTTRKSLRSKGRSRAAPDDSVTLPIYICHDKKIIDSSKKKAE 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            KPL+RNGSSVYSSKRVGSVSETL CKSMEEPAIDEIAIRAVVSILSGYVGRYSKDE+FRE
Sbjct: 61   KPLIRNGSSVYSSKRVGSVSETLLCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDESFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
            I+RKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEG GNERELR+KASRNSIGLLNMV
Sbjct: 121  IMRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGQGNERELRMKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            ITSL+SAK STKNGA SHLS+CAQLYLAIV KIEKNE+VSAKHLLQVFCDSP+FARTHLL
Sbjct: 181  ITSLNSAKKSTKNGAQSHLSSCAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PELWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALSKVYNEHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYF Q SSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFLQSSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420
            R GN+VAA ARSS+EKEIL+S+K+YEN ASV+DEH+NRRM S+LDYR HNT+SWRETVKS
Sbjct: 361  RSGNVVAAKARSSNEKEILYSNKNYENIASVRDEHNNRRMPSILDYRGHNTDSWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480
            DYFRFFTCQNITKEYLESSNVITKNS V+VEGRNHLLSNDFSKAITAICSSD+LSECEIA
Sbjct: 421  DYFRFFTCQNITKEYLESSNVITKNSVVRVEGRNHLLSNDFSKAITAICSSDVLSECEIA 480

Query: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
            +RVVTKAWLDAH D+ IEVALS+PPVVEGMLEVLLASDDDEILELVISVLAELAARSE+I
Sbjct: 481  IRVVTKAWLDAHDDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAARSEVI 540

Query: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            +Q+ILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  RQIILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660
            QTLFS++CKPHEAAFYLLDQLLKGFDEDRNLEN RHLI+LGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSIRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLISLGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720
            SVSIISCCIQADGSCRNYLAENLNKASLLEL+VHESNKNSDR GLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780
            T LLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT LLQLDFMED+LNCSIFREEAIV
Sbjct: 721  TNLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATILLQLDFMEDSLNCSIFREEAIV 780

Query: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840
            TIITALN+RIC EK QDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHS+ 
Sbjct: 781  TIITALNSRICREKGQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSQP 840

Query: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900
            LYDDVVQLYEEEE+V+NWQLKAA+VLFNHGHKSLLS+LST MTSCI  SLAKA LIT+SW
Sbjct: 841  LYDDVVQLYEEEEDVVNWQLKAATVLFNHGHKSLLSALSTLMTSCIL-SLAKASLITVSW 900

Query: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960
            MSRYLFVI++EKLCLMAPSILVPPLIKYLNHDK VEDQVLASYSLLNL KYTECKHIFRL
Sbjct: 901  MSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDQVLASYSLLNLSKYTECKHIFRL 960

Query: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTR 1007
            FD++ LD+L+NLSLVTWTAEEL LI+ S SM PYTE ENS  Q STR
Sbjct: 961  FDEDTLDYLQNLSLVTWTAEELTLILKSRSMHPYTEHENSHIQRSTR 1006

BLAST of CsGy1G033260 vs. NCBI nr
Match: XP_038894767.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1773 bits (4591), Expect = 0.0
Identity = 913/1007 (90.67%), Postives = 962/1007 (95.53%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEGSN+P+TRK  R KGRSR APDDSVTLPIYICHDKK IDSSKKK +
Sbjct: 1    MASLQELLTREGFEGSNFPTTRKSLRSKGRSRAAPDDSVTLPIYICHDKKIIDSSKKKAE 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            KPL+RNGSSVYSSKRVGSVSETL CKSMEEPAIDEIAIRAVVSILSGYVGRYSKDE+FRE
Sbjct: 61   KPLIRNGSSVYSSKRVGSVSETLLCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDESFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
            I+RKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEG GNERELR+KASRNSIGLLNMV
Sbjct: 121  IMRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGQGNERELRMKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            ITSL+SAK STKNGA SHLS+CAQLYLAIV KIEKNE+VSAKHLLQVFCDSP+FARTHLL
Sbjct: 181  ITSLNSAKKSTKNGAQSHLSSCAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PELWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALSKVYNEHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYF Q SSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFLQSSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420
            R GN+VAA ARSS+EKEIL+S+K+YEN AS  DEH+NRRM S+LDYR HNT+SWRETVKS
Sbjct: 361  RSGNVVAAKARSSNEKEILYSNKNYENIAS--DEHNNRRMPSILDYRGHNTDSWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480
            DYFRFFTCQNITKEYLESSNVITKNS V+VEGRNHLLSNDFSKAITAICSSD+LSECEIA
Sbjct: 421  DYFRFFTCQNITKEYLESSNVITKNSVVRVEGRNHLLSNDFSKAITAICSSDVLSECEIA 480

Query: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
            +RVVTKAWLDAH D+ IEVALS+PPVVEGMLEVLLASDDDEILELVISVLAELAARSE+I
Sbjct: 481  IRVVTKAWLDAHDDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAARSEVI 540

Query: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            +Q+ILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  RQIILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660
            QTLFS++CKPHEAAFYLLDQLLKGFDEDRNLEN RHLI+LGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSIRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLISLGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720
            SVSIISCCIQADGSCRNYLAENLNKASLLEL+VHESNKNSDR GLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780
            T LLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT LLQLDFMED+LNCSIFREEAIV
Sbjct: 721  TNLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATILLQLDFMEDSLNCSIFREEAIV 780

Query: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840
            TIITALN+RIC EK QDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHS+ 
Sbjct: 781  TIITALNSRICREKGQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSQP 840

Query: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900
            LYDDVVQLYEEEE+V+NWQLKAA+VLFNHGHKSLLS+LST MTSCI  SLAKA LIT+SW
Sbjct: 841  LYDDVVQLYEEEEDVVNWQLKAATVLFNHGHKSLLSALSTLMTSCIL-SLAKASLITVSW 900

Query: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960
            MSRYLFVI++EKLCLMAPSILVPPLIKYLNHDK VEDQVLASYSLLNL KYTECKHIFRL
Sbjct: 901  MSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDQVLASYSLLNLSKYTECKHIFRL 960

Query: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTR 1007
            FD++ LD+L+NLSLVTWTAEEL LI+ S SM PYTE ENS  Q STR
Sbjct: 961  FDEDTLDYLQNLSLVTWTAEELTLILKSRSMHPYTEHENSHIQRSTR 1004

BLAST of CsGy1G033260 vs. NCBI nr
Match: XP_022142564.1 (putative E3 ubiquitin-protein ligase LIN-1 [Momordica charantia])

HSP 1 Score: 1627 bits (4213), Expect = 0.0
Identity = 841/1007 (83.52%), Postives = 918/1007 (91.16%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEG+NYP TRK SRP+GR RT  D SVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGTNYPKTRKPSRPRGRRRTPADGSVTLPIYICHDKKFIDSSKKKAD 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            K  VRNGSSVYSSKRVGSVSE+  CKSMEEPAID+IAIRAVVSILSGYVG Y+KDE FRE
Sbjct: 61   KAFVRNGSSVYSSKRVGSVSESSLCKSMEEPAIDDIAIRAVVSILSGYVGSYAKDEIFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
             VRKKCN CL+R GEM  GICSNLEMGMKSVDRLVEEGHGNE+ELR+KASRNSIGLLNMV
Sbjct: 121  TVRKKCNLCLVRSGEMGVGICSNLEMGMKSVDRLVEEGHGNEKELRVKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            + SL+S + S +NG+ S LSACA+LYLAIV KIEKNE++SAKHLLQVFCDSP+ ARTHLL
Sbjct: 181  VASLNSVR-SMENGS-SDLSACAKLYLAIVYKIEKNEKISAKHLLQVFCDSPFLARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWY+QELEFVSN ECEHKDR+IKAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PELWEHFFLPHLLHLKVWYSQELEFVSNVECEHKDRRIKALSKVYNEHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGA+APPVPVVPSPSKSIH ASRRSSDSYFSQ SSNKNLYHAVFGPSL++Q+ EL
Sbjct: 301  IQWLKDGAKAPPVPVVPSPSKSIHGASRRSSDSYFSQSSSNKNLYHAVFGPSLEEQVIEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420
            R GN+ AA  RSS+EKEILF++K+YE+SASV +E SN RMSSVLDYR  N +SWRETVKS
Sbjct: 361  RSGNMAAAKTRSSNEKEILFAEKNYESSASVHEELSNHRMSSVLDYRHQNADSWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480
            DY RFFTCQN+ KEYLE+SNVIT+NS V+V+G++HLLSND S+AI+ ICSSDILSECEIA
Sbjct: 421  DYLRFFTCQNVAKEYLENSNVITRNSAVRVDGKSHLLSNDLSRAISTICSSDILSECEIA 480

Query: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
            +RVVTKAWLDAHGD+ IEVALS+PPVVEGMLEVLLASDDDEILELVISVLAELAAR E+I
Sbjct: 481  IRVVTKAWLDAHGDTAIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAARDEVI 540

Query: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            +QMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660
            QTLFSV+CKPHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720
            +VSIISCCIQADGSCRNYL +NLNK SLLEL+VHESNK+SDR GLALLVDLLCLSRRTRI
Sbjct: 661  AVSIISCCIQADGSCRNYLVDNLNKTSLLELIVHESNKSSDRCGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780
            T+LLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVA  LLQLDFMED  NCSI+REEAIV
Sbjct: 721  TRLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAAILLQLDFMEDTQNCSIYREEAIV 780

Query: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840
            TIITAL +RI  E+ Q+NLARALLILGGRF  TGEPS ENWLL  AGFKE+S DS HSKH
Sbjct: 781  TIITALKSRISREEVQENLARALLILGGRFLYTGEPSAENWLLLRAGFKESSEDSFHSKH 840

Query: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900
            +YDD VQ YEEEE+V NWQLKAA+VLFNHGHKSLLS+LS S++S I PSLAKA L+T+SW
Sbjct: 841  VYDDFVQSYEEEEDVENWQLKAATVLFNHGHKSLLSALSISLSSSI-PSLAKASLVTVSW 900

Query: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960
            MS YLFV+R+EKL L+ PSILVPPLIKYLN+DK VED+VLASYSLLNL KYTECKHIFRL
Sbjct: 901  MSTYLFVVRDEKLQLITPSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRL 960

Query: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTR 1007
            FD+E LD L+NLSLVTWTAEEL+LI+ SGS   YT+RENS  Q S R
Sbjct: 961  FDEEVLDRLQNLSLVTWTAEELVLIVRSGSAHQYTKRENSYTQRSRR 1004

BLAST of CsGy1G033260 vs. ExPASy TrEMBL
Match: A0A0A0LYR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G713660 PE=4 SV=1)

HSP 1 Score: 1962 bits (5084), Expect = 0.0
Identity = 1008/1008 (100.00%), Postives = 1008/1008 (100.00%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
            IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV
Sbjct: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL
Sbjct: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420
            RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS
Sbjct: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480
            DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA
Sbjct: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480

Query: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
            VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII
Sbjct: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540

Query: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660
            QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720
            SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780
            TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV
Sbjct: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780

Query: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840
            TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH
Sbjct: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840

Query: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900
            LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW
Sbjct: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900

Query: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960
            MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL
Sbjct: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960

Query: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE 1008
            FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE
Sbjct: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE 1008

BLAST of CsGy1G033260 vs. ExPASy TrEMBL
Match: A0A6J1CLV0 (putative E3 ubiquitin-protein ligase LIN-1 OS=Momordica charantia OX=3673 GN=LOC111012647 PE=4 SV=1)

HSP 1 Score: 1627 bits (4213), Expect = 0.0
Identity = 841/1007 (83.52%), Postives = 918/1007 (91.16%), Query Frame = 0

Query: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60
            MASLQELLTREGFEG+NYP TRK SRP+GR RT  D SVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGTNYPKTRKPSRPRGRRRTPADGSVTLPIYICHDKKFIDSSKKKAD 60

Query: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120
            K  VRNGSSVYSSKRVGSVSE+  CKSMEEPAID+IAIRAVVSILSGYVG Y+KDE FRE
Sbjct: 61   KAFVRNGSSVYSSKRVGSVSESSLCKSMEEPAIDDIAIRAVVSILSGYVGSYAKDEIFRE 120

Query: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180
             VRKKCN CL+R GEM  GICSNLEMGMKSVDRLVEEGHGNE+ELR+KASRNSIGLLNMV
Sbjct: 121  TVRKKCNLCLVRSGEMGVGICSNLEMGMKSVDRLVEEGHGNEKELRVKASRNSIGLLNMV 180

Query: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240
            + SL+S + S +NG+ S LSACA+LYLAIV KIEKNE++SAKHLLQVFCDSP+ ARTHLL
Sbjct: 181  VASLNSVR-SMENGS-SDLSACAKLYLAIVYKIEKNEKISAKHLLQVFCDSPFLARTHLL 240

Query: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWY+QELEFVSN ECEHKDR+IKAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PELWEHFFLPHLLHLKVWYSQELEFVSNVECEHKDRRIKALSKVYNEHMDRGTVQFALYY 300

Query: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGA+APPVPVVPSPSKSIH ASRRSSDSYFSQ SSNKNLYHAVFGPSL++Q+ EL
Sbjct: 301  IQWLKDGAKAPPVPVVPSPSKSIHGASRRSSDSYFSQSSSNKNLYHAVFGPSLEEQVIEL 360

Query: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420
            R GN+ AA  RSS+EKEILF++K+YE+SASV +E SN RMSSVLDYR  N +SWRETVKS
Sbjct: 361  RSGNMAAAKTRSSNEKEILFAEKNYESSASVHEELSNHRMSSVLDYRHQNADSWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480
            DY RFFTCQN+ KEYLE+SNVIT+NS V+V+G++HLLSND S+AI+ ICSSDILSECEIA
Sbjct: 421  DYLRFFTCQNVAKEYLENSNVITRNSAVRVDGKSHLLSNDLSRAISTICSSDILSECEIA 480

Query: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
            +RVVTKAWLDAHGD+ IEVALS+PPVVEGMLEVLLASDDDEILELVISVLAELAAR E+I
Sbjct: 481  IRVVTKAWLDAHGDTAIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAARDEVI 540

Query: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            +QMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660
            QTLFSV+CKPHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720
            +VSIISCCIQADGSCRNYL +NLNK SLLEL+VHESNK+SDR GLALLVDLLCLSRRTRI
Sbjct: 661  AVSIISCCIQADGSCRNYLVDNLNKTSLLELIVHESNKSSDRCGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780
            T+LLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVA  LLQLDFMED  NCSI+REEAIV
Sbjct: 721  TRLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAAILLQLDFMEDTQNCSIYREEAIV 780

Query: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840
            TIITAL +RI  E+ Q+NLARALLILGGRF  TGEPS ENWLL  AGFKE+S DS HSKH
Sbjct: 781  TIITALKSRISREEVQENLARALLILGGRFLYTGEPSAENWLLLRAGFKESSEDSFHSKH 840

Query: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900
            +YDD VQ YEEEE+V NWQLKAA+VLFNHGHKSLLS+LS S++S I PSLAKA L+T+SW
Sbjct: 841  VYDDFVQSYEEEEDVENWQLKAATVLFNHGHKSLLSALSISLSSSI-PSLAKASLVTVSW 900

Query: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960
            MS YLFV+R+EKL L+ PSILVPPLIKYLN+DK VED+VLASYSLLNL KYTECKHIFRL
Sbjct: 901  MSTYLFVVRDEKLQLITPSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRL 960

Query: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTR 1007
            FD+E LD L+NLSLVTWTAEEL+LI+ SGS   YT+RENS  Q S R
Sbjct: 961  FDEEVLDRLQNLSLVTWTAEELVLIVRSGSAHQYTKRENSYTQRSRR 1004

BLAST of CsGy1G033260 vs. ExPASy TrEMBL
Match: A0A1S3C8W1 (putative E3 ubiquitin-protein ligase LIN-2 OS=Cucumis melo OX=3656 GN=LOC103498300 PE=4 SV=1)

HSP 1 Score: 1604 bits (4153), Expect = 0.0
Identity = 823/872 (94.38%), Postives = 849/872 (97.36%), Query Frame = 0

Query: 136  MESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMVITSLDSAKNSTKNGA 195
            MESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMVITSLDSA   TKNGA
Sbjct: 1    MESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMVITSLDSAIKFTKNGA 60

Query: 196  HSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLLPDLWEHFFLPHLLHL 255
            +SHLSACA LYLAIVNKIEK E++SAKHLLQVFCDSP+FARTHLLPDLWEHFFLPHLLHL
Sbjct: 61   YSHLSACAHLYLAIVNKIEKKEKISAKHLLQVFCDSPFFARTHLLPDLWEHFFLPHLLHL 120

Query: 256  KVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYYIQWLKDGARAPPVPV 315
            KVWYNQELEFVSNFECEHKDRK KALNKVYNDHMDRGTVQFALYYIQWLKDGARAPPVPV
Sbjct: 121  KVWYNQELEFVSNFECEHKDRKTKALNKVYNDHMDRGTVQFALYYIQWLKDGARAPPVPV 180

Query: 316  VPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAELRRGNLVAAMARSSHE 375
            V SPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLA LR GNLVAA+ARSS+E
Sbjct: 181  VRSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAGLRSGNLVAALARSSNE 240

Query: 376  KEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQNITKEY 435
            KEILFSDKHYENSAS+QDEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQ+ITKEY
Sbjct: 241  KEILFSDKHYENSASLQDEHSNRRMSSVLDYRSHNTESWRETVKSDYFRFFTCQSITKEY 300

Query: 436  LESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAWLDAHGDS 495
            LESSNVITKNS V+VEGRNHLLSND SKAITAICSSDILSECEIA+RVVTKAWLDAHGD+
Sbjct: 301  LESSNVITKNSSVRVEGRNHLLSNDLSKAITAICSSDILSECEIAIRVVTKAWLDAHGDT 360

Query: 496  NIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSDPQLQVFL 555
             IEVALS+PPVVEGMLEVLLASDDDEILELVIS LAELA RSE+I+QMILNSDPQLQVFL
Sbjct: 361  TIEVALSKPPVVEGMLEVLLASDDDEILELVISFLAELAVRSEVIRQMILNSDPQLQVFL 420

Query: 556  KLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQCKPHEAAF 615
            KLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSV+CKPHEAAF
Sbjct: 421  KLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAF 480

Query: 616  YLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCCIQADGSC 675
            YLLDQLLKGFDEDRNLENSRHLI+LGGLSLLLRRLERGEIEERKNSVSIISCC+QADGSC
Sbjct: 481  YLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKNSVSIISCCVQADGSC 540

Query: 676  RNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLG 735
            RNYLAENLNKASLLEL+VHESNKNS RGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLG
Sbjct: 541  RNYLAENLNKASLLELIVHESNKNSGRGGLALLVDLLCLSRRTRITKLLDGLKEGWSGLG 600

Query: 736  IMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIVTIITALNARICGEKA 795
            IMNILSVYLQRALPEEQPLVAT LLQLDFMEDALNCSIFREEAIVTIITALNARI GEK 
Sbjct: 601  IMNILSVYLQRALPEEQPLVATMLLQLDFMEDALNCSIFREEAIVTIITALNARISGEKV 660

Query: 796  QDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKHLYDDVVQLYEEEEEV 855
            QDNLARALLILGGRFSCTGEPSTENWLLKLAGF+ENSGDSSHSKHLYDDVV LYEEEEEV
Sbjct: 661  QDNLARALLILGGRFSCTGEPSTENWLLKLAGFRENSGDSSHSKHLYDDVVLLYEEEEEV 720

Query: 856  MNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLFVIREEKLCL 915
            +NWQLKAA+VLFNHGHKSLLSSLSTSMTSC+RPSLAKACLITLSWMSRYLFVIR+EKLCL
Sbjct: 721  VNWQLKAATVLFNHGHKSLLSSLSTSMTSCVRPSLAKACLITLSWMSRYLFVIRDEKLCL 780

Query: 916  MAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEALDHLRNLSLV 975
            MAPSILVP LIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFD+EALDHLRNLSLV
Sbjct: 781  MAPSILVPALIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDEEALDHLRNLSLV 840

Query: 976  TWTAEELILIITSGSMDPYTERENSDNQGSTR 1007
            TWTAEELILIITSGSM P+TE+ENS  QGSTR
Sbjct: 841  TWTAEELILIITSGSMHPHTEQENSHTQGSTR 872

BLAST of CsGy1G033260 vs. ExPASy TrEMBL
Match: A0A6J1JWN9 (putative E3 ubiquitin-protein ligase LIN isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490369 PE=4 SV=1)

HSP 1 Score: 1520 bits (3936), Expect = 0.0
Identity = 805/1008 (79.86%), Postives = 879/1008 (87.20%), Query Frame = 0

Query: 1   MASLQELLTREGFEGSNYPSTRKLSRPKG------RSRTAPDDSVTLPIYICHDKKTIDS 60
           MASLQELLTREGFEG N+ + RK SRP G      R RT  D SVTLPIYICHDKK IDS
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGRRRRRRTVSDGSVTLPIYICHDKKIIDS 60

Query: 61  SKKKHDKPLVRNGSSVYSSKRVGSVSETLPCKSME-----EPAIDEIAIRAVVSILSGYV 120
           SKKK DK LVRNGSSVYSSKRVGSVSET  CKSME     EPAID IAIRAVVSILSGYV
Sbjct: 61  SKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSILSGYV 120

Query: 121 GRYSKDENFREIVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKA 180
           GRY KDE+FRE+VRKKC  C         GIC NLEMGMKSVDRLVEE +GNE+ELR+KA
Sbjct: 121 GRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEKELRVKA 180

Query: 181 SRNSIGLLNMVITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFC 240
           SRNSIGLLNMVI    SA  + KNG  SHLSACA LYLAIV KIEKNE+VSAKH+LQ+FC
Sbjct: 181 SRNSIGLLNMVI----SATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFC 240

Query: 241 DSPYFARTHLLPDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHM 300
           D P+FARTHLLP+LWEHFFLPHLLHLKVWYNQELEFVSN + EHK+R IKAL+KVYN++M
Sbjct: 241 DYPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYM 300

Query: 301 DRGTVQFALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSS-NKNLYHAV 360
           D GT QFA+YYIQWLKDGA+APPVPVV  PSKSIH  SRRSSDSYFSQ SS NKNLYHAV
Sbjct: 301 DMGTAQFAVYYIQWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSNKNLYHAV 360

Query: 361 FGPSLDQQLAELRRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRS 420
           FGPSLDQQLAELR     AA ARSS+E           +S  V DE SN   SS+L Y++
Sbjct: 361 FGPSLDQQLAELRS----AAKARSSNE-----------SSVPVHDELSNGITSSILRYKT 420

Query: 421 HNTESWRETVKSDYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAI 480
           HNT+SWRETVKSDYF FFTCQNITKE+ ESSN++ KN+ ++VEGRNH LS+  S+AI  I
Sbjct: 421 HNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKNA-IRVEGRNHNLSSVVSRAIATI 480

Query: 481 CSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVIS 540
           CSSDIL++CEIAVRVVTKAWLDAHGD+ IEVALS+PPV+EGMLEVLLAS+DDEILELVIS
Sbjct: 481 CSSDILNDCEIAVRVVTKAWLDAHGDAAIEVALSKPPVIEGMLEVLLASNDDEILELVIS 540

Query: 541 VLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLP 600
           VLAELAA++EII+Q+IL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEWLP
Sbjct: 541 VLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLP 600

Query: 601 LVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLR 660
           LVLRVLEFGGQLQTLFSV+C PHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLR
Sbjct: 601 LVLRVLEFGGQLQTLFSVRCMPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLR 660

Query: 661 RLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALL 720
           RLE GEIEERK SVSII CCI+ADGSCRNYLAEN+NKASLLEL+VHESN NSDR GLALL
Sbjct: 661 RLEEGEIEERKKSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLALL 720

Query: 721 VDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDA 780
           VDLLCLSRRTRI++LLDGLK+GW+GL IMN+LSVYLQRA PEEQPLVAT LLQLDFMED 
Sbjct: 721 VDLLCLSRRTRISRLLDGLKDGWNGLSIMNVLSVYLQRARPEEQPLVATILLQLDFMEDP 780

Query: 781 LNCSIFREEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGF 840
           LNC+IFREEAIVTIIT+LNARI  EK Q+NLARALLILGGRFSCTGEP+ ENWLL+ AGF
Sbjct: 781 LNCTIFREEAIVTIITSLNARISHEKTQENLARALLILGGRFSCTGEPTIENWLLQQAGF 840

Query: 841 KENSGDSSHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRP 900
           KENSG S HSKH+YDD VQ YEEEEEV+NWQLKAA+V FNHGHKSLLS+LSTSMTSCI P
Sbjct: 841 KENSGGSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCI-P 900

Query: 901 SLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNL 960
           SLAKACL+ +SWMS+YLFV+ ++KLCLMAPSILVPPLIKYLN+DK VEDQVLASYSLLNL
Sbjct: 901 SLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKEVEDQVLASYSLLNL 960

Query: 961 GKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTE 996
            KYTECKHIFRLFD+EALDHLRNLSLVTWTAEELI I+ SGS   YTE
Sbjct: 961 SKYTECKHIFRLFDEEALDHLRNLSLVTWTAEELISIVESGSTHQYTE 978

BLAST of CsGy1G033260 vs. ExPASy TrEMBL
Match: A0A6J1GRP8 (putative E3 ubiquitin-protein ligase LIN OS=Cucurbita moschata OX=3662 GN=LOC111456892 PE=4 SV=1)

HSP 1 Score: 1496 bits (3873), Expect = 0.0
Identity = 793/1010 (78.51%), Postives = 875/1010 (86.63%), Query Frame = 0

Query: 1   MASLQELLTREGFEGSNYPSTRKLSRPKG-------RSRTAPDDSVTLPIYICHDKKTID 60
           MASLQELLTREGFEG N+ + RK  RP G       R RT  D SVTLPIYICHDKK ID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPLRPNGGGGGRRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKHDKPLVRNGSSVYSSKRVGSVSETLPCKSME-----EPAIDEIAIRAVVSILSGY 120
           SSKKK DK LVRNGSSVYSSKRVGSVSET  CKSME     EPAID IAIRAVVS+LSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120

Query: 121 VGRYSKDENFREIVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIK 180
           VGRY KDE+FRE+VRKKC  C         GIC NLEMGMKSVDRLVEE +GNE+ELR+K
Sbjct: 121 VGRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEQELRVK 180

Query: 181 ASRNSIGLLNMVITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVF 240
           ASRNSIGLLNMVI    SA  + KNG  SHLSACA LYLAIV KIEKNE+VSAKH+LQ+F
Sbjct: 181 ASRNSIGLLNMVI----SATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLF 240

Query: 241 CDSPYFARTHLLPDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDH 300
           CD P+FART+LLP+LWEHFFLPHLLHLKVWYNQELEFVSN + EHK+R IKAL+KVYN++
Sbjct: 241 CDYPFFARTYLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEY 300

Query: 301 MDRGTVQFALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSS-NKNLYHA 360
           MD GT QFA+YY+QWLKDGA+APPVPVV  PSKSI   SRRSSDSYFSQ SS NKNLYHA
Sbjct: 301 MDMGTAQFAVYYVQWLKDGAKAPPVPVVSLPSKSISGMSRRSSDSYFSQSSSSNKNLYHA 360

Query: 361 VFGPSLDQQLAELRRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYR 420
           VFGPSLDQQLAELR     AA ARSS+EK +            V DE SN   SS+L Y+
Sbjct: 361 VFGPSLDQQLAELR----TAAKARSSNEKSVP-----------VHDEPSNGISSSILCYK 420

Query: 421 SHNTESWRETVKSDYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITA 480
           +HNT+SWRETVKSDYF FFTCQNITKE+ ESSN++ KN+ ++VEGRNH LS+  ++AI  
Sbjct: 421 THNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKNA-IRVEGRNHNLSSVVNRAIAT 480

Query: 481 ICSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVI 540
           ICSSDIL++CEIAVRVVTKAWLDAHGD+ IE  LS+P VVEGMLEVLLAS+DDEILELVI
Sbjct: 481 ICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAVLSKPLVVEGMLEVLLASNDDEILELVI 540

Query: 541 SVLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWL 600
           SVLAELAA++EII+Q+IL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEWL
Sbjct: 541 SVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEWL 600

Query: 601 PLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLL 660
           PLVLRVLEFGGQLQTLFSV+C PHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLL
Sbjct: 601 PLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL 660

Query: 661 RRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLAL 720
           RRLE GEIEERKNSVSII CCI+ADGSCRNYLAEN+NKASLLEL+VHESN NSDR GLAL
Sbjct: 661 RRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLAL 720

Query: 721 LVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMED 780
           LVDLLCLSRR RI++LLDGLK+GW+GL IMN+LS+YLQRA PEEQPLVAT LLQLDFMED
Sbjct: 721 LVDLLCLSRRARISRLLDGLKDGWNGLSIMNVLSIYLQRARPEEQPLVATILLQLDFMED 780

Query: 781 ALNCSIFREEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAG 840
            LNC+IFREEAIVTIIT+LNARI  EK ++NLARALLILGGRFSCTGEP+ ENWLL+ AG
Sbjct: 781 PLNCTIFREEAIVTIITSLNARISREKTRENLARALLILGGRFSCTGEPTIENWLLQQAG 840

Query: 841 FKENSGDSSHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIR 900
           FKE+SGDS HSKH+YDD VQ YEEEEEV+NWQLKAA+V FNHGHKSLLS+LSTSMTSCI 
Sbjct: 841 FKESSGDSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCI- 900

Query: 901 PSLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLN 960
           PSLAKACL+ +SWMS+YLFV+ ++KLCLMAPSILVPPLIKYLN+DK VED+VLASYSLLN
Sbjct: 901 PSLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKDVEDRVLASYSLLN 960

Query: 961 LGKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELILIIT-SGSMDPYTE 996
           L KYTECKHIFRLFD+EAL+HLRNLSLVTWTAEELI I+  SGS   YTE
Sbjct: 961 LSKYTECKHIFRLFDEEALEHLRNLSLVTWTAEELISIVEMSGSTHQYTE 980

BLAST of CsGy1G033260 vs. TAIR 10
Match: AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 4.4e-19
Identity = 121/515 (23.50%), Postives = 232/515 (45.05%), Query Frame = 0

Query: 481 VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
           V  VT  +   +     + ++ +  +++ +   + +S++  +++  ++ L ++ + +   
Sbjct: 461 VSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIISVNRTA 520

Query: 541 QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            + +      L      LK +    +A+IL+YL KP   ++ S+E LP ++ V+      
Sbjct: 521 LEEVKRKGLNLSHLANALKQN--VQEAAILIYLIKPSPTEIKSLELLPALVDVVASTSSS 580

Query: 601 QTLFSV-----QCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLE---R 660
            + ++         P  A+  +++ L+  FD   +  N+ HL A+   S+L   L+    
Sbjct: 581 SSCYTFIPSPSYLTPPAASLMIIEVLITAFD---HATNTMHLAAISSPSVLCGLLDVAKS 640

Query: 661 GEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLAL--LVD 720
           G   E  +  SI+  C+Q DG  R Y+ ++   A    L+    +K+ +   +AL  L +
Sbjct: 641 GNSGEFISLTSILVKCMQFDGLHRKYIYQHTRVAPFAHLL---QSKDQEEICIALQFLHE 700

Query: 721 LLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALN 780
           +L + R + I  L    KEG     I   L   ++    + +   A  LLQL+ ++    
Sbjct: 701 VLKIPRSSAIKILQQIKKEG--SFDIKVTLLHCIKHLQGDHKLFAADILLQLNALDSPPE 760

Query: 781 CSIFREEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKE 840
              +R EA   ++ A+     G   Q      L  +GG +S TGEP T  WL+K  G   
Sbjct: 761 NKKYRNEATRALLDAVTYSE-GSNMQLLSTFILANIGGTYSWTGEPYTAAWLMKRGGLTS 820

Query: 841 NSG-DSSHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPS 900
            S  +   + +  D+ +Q    +  +  W  K A  + + G K+    L   + S    S
Sbjct: 821 MSHMNMIRNINWSDECLQ----DTGIDGWCCKIARRIIDTG-KATFCGLQEGLKS-KNKS 880

Query: 901 LAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLG 960
           ++KACLI ++W+S  +       L   A  +L+  + ++L+    +E+++LA   + N  
Sbjct: 881 VSKACLIAIAWLSIEISK-GPNSLKYSACEVLLDEVAQFLHPGLELEERLLACICIYN-- 940

Query: 961 KYTECKHIFRL--FDDEALDHLRNLSLVTWTAEEL 983
            ++  K I +L  F +   + LR LS VTW A+EL
Sbjct: 941 -FSSGKGIHKLVNFSEGVRESLRRLSHVTWMADEL 954

BLAST of CsGy1G033260 vs. TAIR 10
Match: AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 4.4e-19
Identity = 121/515 (23.50%), Postives = 232/515 (45.05%), Query Frame = 0

Query: 481 VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540
           V  VT  +   +     + ++ +  +++ +   + +S++  +++  ++ L ++ + +   
Sbjct: 461 VSEVTMIYQMLNRKEGFKYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIISVNRTA 520

Query: 541 QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            + +      L      LK +    +A+IL+YL KP   ++ S+E LP ++ V+      
Sbjct: 521 LEEVKRKGLNLSHLANALKQN--VQEAAILIYLIKPSPTEIKSLELLPALVDVVASTSSS 580

Query: 601 QTLFSV-----QCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLE---R 660
            + ++         P  A+  +++ L+  FD   +  N+ HL A+   S+L   L+    
Sbjct: 581 SSCYTFIPSPSYLTPPAASLMIIEVLITAFD---HATNTMHLAAISSPSVLCGLLDVAKS 640

Query: 661 GEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLAL--LVD 720
           G   E  +  SI+  C+Q DG  R Y+ ++   A    L+    +K+ +   +AL  L +
Sbjct: 641 GNSGEFISLTSILVKCMQFDGLHRKYIYQHTRVAPFAHLL---QSKDQEEICIALQFLHE 700

Query: 721 LLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALN 780
           +L + R + I  L    KEG     I   L   ++    + +   A  LLQL+ ++    
Sbjct: 701 VLKIPRSSAIKILQQIKKEG--SFDIKVTLLHCIKHLQGDHKLFAADILLQLNALDSPPE 760

Query: 781 CSIFREEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKE 840
              +R EA   ++ A+     G   Q      L  +GG +S TGEP T  WL+K  G   
Sbjct: 761 NKKYRNEATRALLDAVTYSE-GSNMQLLSTFILANIGGTYSWTGEPYTAAWLMKRGGLTS 820

Query: 841 NSG-DSSHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPS 900
            S  +   + +  D+ +Q    +  +  W  K A  + + G K+    L   + S    S
Sbjct: 821 MSHMNMIRNINWSDECLQ----DTGIDGWCCKIARRIIDTG-KATFCGLQEGLKS-KNKS 880

Query: 901 LAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLG 960
           ++KACLI ++W+S  +       L   A  +L+  + ++L+    +E+++LA   + N  
Sbjct: 881 VSKACLIAIAWLSIEISK-GPNSLKYSACEVLLDEVAQFLHPGLELEERLLACICIYN-- 940

Query: 961 KYTECKHIFRL--FDDEALDHLRNLSLVTWTAEEL 983
            ++  K I +L  F +   + LR LS VTW A+EL
Sbjct: 941 -FSSGKGIHKLVNFSEGVRESLRRLSHVTWMADEL 954