CsGy1G032850 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G032850
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionChaperonin 60 subunit alpha 2
LocationGy14Chr1: 32541209 .. 32546883 (-)
RNA-Seq ExpressionCsGy1G032850
SyntenyCsGy1G032850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAACCATGAAGATCCGTATGAATAAATAGAAAACCCCTCCTAAACCCTGACGAAACTTTGGGATGAACTTGAACTACCCACTCATCTCTTCTTCTCTTCTCTACGACGCCAATAACCTCATGGAATTCTCTGCCCGTCTCTCCTCACCGGCTCCCTTTCCCCAAACTCTACTCTTCCCTCTGATGGTACACCTTTTTCTTTCTCTTTTACCGCACATCTTCTTAATTCAGTCACTTTGACTTTATGGCTGTTCTGCAGAAGCACGGTGGGAGTCAAAGGCTATCTGGGTACGCGAGAAATTCATGGAATATCAGAAACTTTGTCGTTAGGGCCGGTCCTAAGAGGATATCTTTTGGTAAAGAATGCAGAGGGGCCTTGCTAGCTGGTATTGATAAGTTGGCTGATGCTGTTTCGGTCACTTTAGGACCTAAAGGTTTGTACAACTTCCTATTTTCTTTGATTCTACACCATTTTTAGATGCATTATTTATTTCTTGCGGAATTTTGTTTAATATCACATGTTTGTGTGTGTTTGGGAAGCCAGTGTCTAATTCTTCGCATTCTCAATTTGCTGGTTAATTGTGCGCTGGACTTAAAGTGGCGAGTTTATGATGTCGTTTCTGTAAAGATAATGGTTTTGCATTATATTTTAATTAGTCAATCACTTCTAATTTGAAGAAATTCAGAAGATAGTGTATGAGCATGTGAAAACACAATTTGTTGGAGTTGCAATTTCTGAAGTGTGTGAAGGAAAAGAGGAAAAGATTGGCCATTTCAAAATTCTCTCCTCCCGTGTAATTTCATTTGATTTGGGTGAAGAACTCTTGACCACTTAAATCTGGAACTGTTTGGTGATAATTACTTATTTATTGTTCAATCTAGTTACCCTGTGAATGGTTCAAGAATGATGCATAATATCCTGTCCATTTTAGATATTTTAGTTCTTTTTCACTAAAACATGCAGGCCGCAATGTTATTCTCTCTGAACAGAAAACGCTTAAAGTGGTTAATGATGGGGTTACAATTGCAAAAGCTATCGAGCTTTCTGATGCAATTGAGAATGCAGGAGTGGTTCTGATCCAAGAGGTATGATTGTTAATTAAAATCTTATTTTTTCTGAAACTTGTTTGATGGGGTTTAACATCTAAGATATCTTTGAGTTATCGGTTTTCAGGTTGCTAGTAAAATGAACGACTTGGCTGGAGATGGAACCACCACCGCTATAATTTTGGCACGTGAAATGATCAAATCCGGATTATTGGCAGTTTCCTTTGGGGCTGATCCAGTTTCATTAAAGAAAGGAATGGATAAGACTGTAAAGGAGTTGATCAAGGTTTTAAAGAAGAAAAGTACTCCTGTACAAGGAAAAGATGATATTAAAGGTTCTTTCTTCTCATCTGGTTGAATAATGAACACTGGTGATGTTTCATCTGAGAAGTAAATGGTGCTGAACTAGCCTCGTCTTTTGTGCAGCTGTTGCAATGATATCTTCTGGAAATGATGAATATGTGGGGAACTTAATTGCTGAAGCAATTGAAAAGATTGGCCCTGATGGTGTGATCTCAATTGAGTCATCAAAATCCTCTGAAACTTCGGTAATAATAGAGGAAGGAATGAAGGTAAATGATTTAGCTGCAAAGATGGTCAGTTTTTTTTTACAACCAATTGAGTAGTGCCTATTTGATGTGCATCTGCAATATTCAAAATTTATCTCATTGTTTTTTCATTTTGCCTTATGGTTCCAACTTCCGTTGTATCTCTCTGCACAGATTGATAAAGGTTATATGTCGCCTCAGTTTATTACCAACCAAGATAAGTCTATTGTGGAGTTTGACAATGCAAAAGTCCTAGTAACCGATCAAAGGATTTCTTCTGTCAAAGAAATTGTTCCTTTGTTGGAGAAGACTGTACAACTTAGTCTTCCTCTGCTGATATTTGCAGAGGACATCTCAATAAAAGTTCTAGAGACCCTAGTGGTGAACAAGATACAGGGTTTAGTTAATGTGGCTGTTGTAAAATGTCCTGGGGTTGGTGAGAGAAAGAAGGCTTTATTGCAAGATATTGCGCTGATGACAGGTGAACTAAATACTTTTATAAGCAGTTATCATACACTCACTTAAATGATTGTTATTTCAGTGGCGGATCTAAGCAATAAAAATCTATCCAAAACTTGGCTTGCATTTGTATATTTGCTTCAGGGTTTTGGAAGAGAGGAGGCTTTTTGGTTAAGTGATATTATTCATCAAATTTTATTTAACTATATTAAACAGTGTGATGGCACTGTGAATATATCGTTTTTAACAAAGACAACAGCTTTTGATTTGCAGTTCAGATTTTTTTAGTAGCACTTGAATGGCCTGAGTACATTCTTAGGCTTTCATTTTATCACTTGTACTGCTTTCTCCTAAAGTTGTGTAGCATACATTTGGTTGCTGGAAAAGATCCTAGAGTATTGTTGGGATGTGTCCAACTTTTCATACAGCTTGAAAGCTATCATGATTAGTTGCGATGGTAAAAACTCAATTTAAATCTCTCAGGTTTATTTTTCAGAGTGTAAAATCTTATACATAGTTTAAAATCTGGAAGGATCTGATTGGCGGAGGTGATTATCTGCTGTGTGATGTTAGATTGCAGTTGATATGACTATCTGATTATGTTACAGTACAATATTAGATTGTTGACTTTATTTTAAAATTGTCAGGTGCTGATTTTCTCTCTGGAGATTTGGGTCTAGGACTTGAGGGTGCAACTTCGGACCAGCTTGGAATTGCTAGGAAAGTAGTTATAACATCTAATTCAACAACAATAGTTGCAGATCCCTCTACTAAAGATGAAATTCAAGCAAGAATTTCACAGATTAAGAAGGATCTTGTTGAAACAGATAATCCCAACCTCTCAAGAAAGCTGTCGGAGAGGATTGCTAAACTTTCTGGTGGAGTTGCTGTCATCAAGGTTTGTTGTTTTAAAAGATTACTTAAGGGTGTAGCTTTTGAGAGGCTTCTCATAATTCATTTGACTGCATGATTTTTCTGTATTCATACACTGGTTCGGACTTCCTGAAATTTAATTCCTATTCTTATTAGCTATGCGAACCTAGCTCATGAGTCATGATATTAACTGAAAACAGATTACCTGTTAGTTCAGTAGTTAAATGGAAGTATGGAAGTCAGAAGTATCTTATATATCAAAGGGAGAGATGCCTCAAAAGCAACAGTTTCTTGAAGATCCTCCACGTTACCTTCTTTTCCTTGGAATACTTTTACACCCTCTTTTCCATGCTCACAGACAAAGATTTTAGAGTGCCAATGAGAGAGCTTTGGGGAAGTGTTTAAACCCTAGAGAAGCAAGGTCGAGAAGAAAGCCTTGTACATCTTAAGATTAGCTAGCTTTCCAAATAGCCTTAGAGAGCTGAAAGTTAAATGTTTCTTGTTCCATCGTGAGCATGCCAAGAGGGGACCAGGAGTCCAGGACATTAAAGAGAAGGAAGATGAAGAATTGGGAAACCAAACTCTACCATTCTCATTCAAATACCTGAAATTGCCCCCTGCTTCTCTTTATCGGATTTCTTTGAAAAGTTCTGAAATCTGTTATTCATGTTCTGTATTTGGGCAAGGCAGTATAGTACAACTCGCCGTACAGCCTTGGAAGCATAGTGCATAAAGGAGCGTTATGTATCCTAAACTTCCAAATTTCTCTGTTGAACAATTAATACTTGATAGCATGGGATTTTAGATTAATATACAATTCAAAGATCTTATCTAGATGAAATGAAAGTTCATCTTAGCTTAACCAAGTCCTCAATTTATGACTATTGCATTGCATGTTGTTCTGTAGTGTGTGAGAAGATTGAGGATATTCTTTTAATCCACCCAAGAACCAAAACAGCAAAAAATAATTATTAGAAAGCTCTGAATTTTTATTTGACTGAAAGACATTTATGAAAAGAAAATTGTAATCAAGAATTCGTCATCGTTCTGACTTTACTATGCATCAGTGACTAATCATTTAAAGGGCTTCTTTGCTGTTTAATTTTTTCAGAATTGGGCTTTGAATTGTCTTAACTAATATTATTGTCTTTTTTTTGTTTCTTACATGTCACATCTTATCTTATTTTCTTTATGAACAGTAAAACATCCAAGGAATAAAAATATTTTCTTTTACATTTTTTTTATTGTATGTGGTAATGTGGTCAAAGTTAGGTGGGAGCACATACAGAGGTGGAACTTGAAGACAGAAAACTTAGAATTGAGGATGCAAAGAATGCTGTATTTGCTGCTATGAATGAAGGCATTGTTCCTGGTGGAGGTGCCACCTATGTTCATCTGTATGAATTGCTTCCTACCATAAAACAATCAATGGAAGATCAAGATGAGCTGATTGGTGCTGATATTGTGGGCAAGGTATTGTCAATGCCCCTTTTCATTGTCGTGGTTTCCTTCTCTCTTCAATTTTTTCGTGGAAATTGAACTGCTATTACTTTAGTACCTTTTTGATAGTGAAATGGAAGTTTTGATTTGGTAGATAATCCAAGTATTCTATAATGAAGAAGATGAATGGTGAATTTAAGTTCAGGCACAATATTTCGGTTTTTCTTGGAATTAGCAAGGCATGCCATGTATCGATTGCTTGTAGAAGTGCTCCTTTCATCAAAAATTTGTGCGCCTTTCAAAATTTTGAATAGATTTTGCAAAAAAAATTGCAGGCACTTCTTGCTCCTGCAAAACTCATTGCTAGTAATGCAGGGGATGATGGAGTAGTTGTTGTGGAGAAAACTCGAGCATGCGATTGGCGACACGGGTATAATGCAATGACAGACAAATATGAAGATCTTTTCAATGCTGGTGTAGTAGATCCTTGCCTTGTTTCCAGATGTGCGCTTCAGATTGCAGCCTCAGTTACCGGAATCGTTTTAACCACTCAAGCTGTAATGGTAGAGAAAATAAAGAAGCCAAAGCCACTCGTTCCCCATGTTCCTGGAATATCTCCATGAATGGAAAAGAAAAGAGAAATAGATATTCCCAAAAGTTGACATGAAAAGACAAAAGAAGAGATCGATCGACCTCGCCATGGTTTCTCTTGGAACCTTCTGAGCTTGAGTTGAATAAGGTAAAGAGATTAAGAAGCTTTTAGGGTTTCATGAAGCAGTTGGGAAAGCTCCCATTTTCACCTGTGGCTGATTAAGAGATGACTTGCCCAATTGCTAAGCTAATATAATGAAAGAAATTAATTTGTAGGAACTGGGTTAGCTCCTCTAGTTTTCGTCTCCTAATTATTGCAAGAAAAATACCTATTAAAGCAGAAAAGCTATCCATGTTTACTTTATTACCACAGTGTTGGTTCTGTTTCCAGGTTTTCCTGTTGTAAATTATCAACATTTTGTAATGACTGTTCTATTATCTTTCATAGAAATGATAACATTGGGCCAAATTTGACATGGAGTGGACTTGTCCCAACCCAAACTAGCAATATCAATGACCAAGTTAGACTGAAACCAAACATGTCTGACTGAAACCAATAAAAACTAAAAGAATTCTTAGCTTCATTCATTTGGATCCTTGTCAAAACTTTGGTACTTTTTACTTTAGTCTATTTTAGATCTACGTACCCTCAGAATGTTTATTTTGGTCCATGTTAAAAACGATTTGTGTTTATGTTAGTCAGTC

mRNA sequence

CGAACCATGAAGATCCGTATGAATAAATAGAAAACCCCTCCTAAACCCTGACGAAACTTTGGGATGAACTTGAACTACCCACTCATCTCTTCTTCTCTTCTCTACGACGCCAATAACCTCATGGAATTCTCTGCCCGTCTCTCCTCACCGGCTCCCTTTCCCCAAACTCTACTCTTCCCTCTGATGAAGCACGGTGGGAGTCAAAGGCTATCTGGGTACGCGAGAAATTCATGGAATATCAGAAACTTTGTCGTTAGGGCCGGTCCTAAGAGGATATCTTTTGGTAAAGAATGCAGAGGGGCCTTGCTAGCTGGTATTGATAAGTTGGCTGATGCTGTTTCGGTCACTTTAGGACCTAAAGGCCGCAATGTTATTCTCTCTGAACAGAAAACGCTTAAAGTGGTTAATGATGGGGTTACAATTGCAAAAGCTATCGAGCTTTCTGATGCAATTGAGAATGCAGGAGTGGTTCTGATCCAAGAGGTTGCTAGTAAAATGAACGACTTGGCTGGAGATGGAACCACCACCGCTATAATTTTGGCACGTGAAATGATCAAATCCGGATTATTGGCAGTTTCCTTTGGGGCTGATCCAGTTTCATTAAAGAAAGGAATGGATAAGACTGTAAAGGAGTTGATCAAGGTTTTAAAGAAGAAAAGTACTCCTGTACAAGGAAAAGATGATATTAAAGCTGTTGCAATGATATCTTCTGGAAATGATGAATATGTGGGGAACTTAATTGCTGAAGCAATTGAAAAGATTGGCCCTGATGGTGTGATCTCAATTGAGTCATCAAAATCCTCTGAAACTTCGGTAATAATAGAGGAAGGAATGAAGATTGATAAAGGTTATATGTCGCCTCAGTTTATTACCAACCAAGATAAGTCTATTGTGGAGTTTGACAATGCAAAAGTCCTAGTAACCGATCAAAGGATTTCTTCTGTCAAAGAAATTGTTCCTTTGTTGGAGAAGACTGTACAACTTAGTCTTCCTCTGCTGATATTTGCAGAGGACATCTCAATAAAAGTTCTAGAGACCCTAGTGGTGAACAAGATACAGGGTTTAGTTAATGTGGCTGTTGTAAAATGTCCTGGGGTTGGTGAGAGAAAGAAGGCTTTATTGCAAGATATTGCGCTGATGACAGGTGCTGATTTTCTCTCTGGAGATTTGGGTCTAGGACTTGAGGGTGCAACTTCGGACCAGCTTGGAATTGCTAGGAAAGTAGTTATAACATCTAATTCAACAACAATAGTTGCAGATCCCTCTACTAAAGATGAAATTCAAGCAAGAATTTCACAGATTAAGAAGGATCTTGTTGAAACAGATAATCCCAACCTCTCAAGAAAGCTGTCGGAGAGGATTGCTAAACTTTCTGGTGGAGTTGCTGTCATCAAGGTGGGAGCACATACAGAGGTGGAACTTGAAGACAGAAAACTTAGAATTGAGGATGCAAAGAATGCTGTATTTGCTGCTATGAATGAAGGCATTGTTCCTGGTGGAGGTGCCACCTATGTTCATCTGTATGAATTGCTTCCTACCATAAAACAATCAATGGAAGATCAAGATGAGCTGATTGGTGCTGATATTGTGGGCAAGGCACTTCTTGCTCCTGCAAAACTCATTGCTAGTAATGCAGGGGATGATGGAGTAGTTGTTGTGGAGAAAACTCGAGCATGCGATTGGCGACACGGGTATAATGCAATGACAGACAAATATGAAGATCTTTTCAATGCTGGTGTAGTAGATCCTTGCCTTGTTTCCAGATGTGCGCTTCAGATTGCAGCCTCAGTTACCGGAATCGTTTTAACCACTCAAGCTGTAATGGTAGAGAAAATAAAGAAGCCAAAGCCACTCGTTCCCCATGTTCCTGGAATATCTCCATGAATGGAAAAGAAAAGAGAAATAGATATTCCCAAAAGTTGACATGAAAAGACAAAAGAAGAGATCGATCGACCTCGCCATGGTTTCTCTTGGAACCTTCTGAGCTTGAGTTGAATAAGGTAAAGAGATTAAGAAGCTTTTAGGGTTTCATGAAGCAGTTGGGAAAGCTCCCATTTTCACCTGTGGCTGATTAAGAGATGACTTGCCCAATTGCTAAGCTAATATAATGAAAGAAATTAATTTGTAGGAACTGGGTTAGCTCCTCTAGTTTTCGTCTCCTAATTATTGCAAGAAAAATACCTATTAAAGCAGAAAAGCTATCCATGTTTACTTTATTACCACAGTGTTGGTTCTGTTTCCAGGTTTTCCTGTTGTAAATTATCAACATTTTGTAATGACTGTTCTATTATCTTTCATAGAAATGATAACATTGGGCCAAATTTGACATGGAGTGGACTTGTCCCAACCCAAACTAGCAATATCAATGACCAAGTTAGACTGAAACCAAACATGTCTGACTGAAACCAATAAAAACTAAAAGAATTCTTAGCTTCATTCATTTGGATCCTTGTCAAAACTTTGGTACTTTTTACTTTAGTCTATTTTAGATCTACGTACCCTCAGAATGTTTATTTTGGTCCATGTTAAAAACGATTTGTGTTTATGTTAGTCAGTC

Coding sequence (CDS)

ATGAACTTGAACTACCCACTCATCTCTTCTTCTCTTCTCTACGACGCCAATAACCTCATGGAATTCTCTGCCCGTCTCTCCTCACCGGCTCCCTTTCCCCAAACTCTACTCTTCCCTCTGATGAAGCACGGTGGGAGTCAAAGGCTATCTGGGTACGCGAGAAATTCATGGAATATCAGAAACTTTGTCGTTAGGGCCGGTCCTAAGAGGATATCTTTTGGTAAAGAATGCAGAGGGGCCTTGCTAGCTGGTATTGATAAGTTGGCTGATGCTGTTTCGGTCACTTTAGGACCTAAAGGCCGCAATGTTATTCTCTCTGAACAGAAAACGCTTAAAGTGGTTAATGATGGGGTTACAATTGCAAAAGCTATCGAGCTTTCTGATGCAATTGAGAATGCAGGAGTGGTTCTGATCCAAGAGGTTGCTAGTAAAATGAACGACTTGGCTGGAGATGGAACCACCACCGCTATAATTTTGGCACGTGAAATGATCAAATCCGGATTATTGGCAGTTTCCTTTGGGGCTGATCCAGTTTCATTAAAGAAAGGAATGGATAAGACTGTAAAGGAGTTGATCAAGGTTTTAAAGAAGAAAAGTACTCCTGTACAAGGAAAAGATGATATTAAAGCTGTTGCAATGATATCTTCTGGAAATGATGAATATGTGGGGAACTTAATTGCTGAAGCAATTGAAAAGATTGGCCCTGATGGTGTGATCTCAATTGAGTCATCAAAATCCTCTGAAACTTCGGTAATAATAGAGGAAGGAATGAAGATTGATAAAGGTTATATGTCGCCTCAGTTTATTACCAACCAAGATAAGTCTATTGTGGAGTTTGACAATGCAAAAGTCCTAGTAACCGATCAAAGGATTTCTTCTGTCAAAGAAATTGTTCCTTTGTTGGAGAAGACTGTACAACTTAGTCTTCCTCTGCTGATATTTGCAGAGGACATCTCAATAAAAGTTCTAGAGACCCTAGTGGTGAACAAGATACAGGGTTTAGTTAATGTGGCTGTTGTAAAATGTCCTGGGGTTGGTGAGAGAAAGAAGGCTTTATTGCAAGATATTGCGCTGATGACAGGTGCTGATTTTCTCTCTGGAGATTTGGGTCTAGGACTTGAGGGTGCAACTTCGGACCAGCTTGGAATTGCTAGGAAAGTAGTTATAACATCTAATTCAACAACAATAGTTGCAGATCCCTCTACTAAAGATGAAATTCAAGCAAGAATTTCACAGATTAAGAAGGATCTTGTTGAAACAGATAATCCCAACCTCTCAAGAAAGCTGTCGGAGAGGATTGCTAAACTTTCTGGTGGAGTTGCTGTCATCAAGGTGGGAGCACATACAGAGGTGGAACTTGAAGACAGAAAACTTAGAATTGAGGATGCAAAGAATGCTGTATTTGCTGCTATGAATGAAGGCATTGTTCCTGGTGGAGGTGCCACCTATGTTCATCTGTATGAATTGCTTCCTACCATAAAACAATCAATGGAAGATCAAGATGAGCTGATTGGTGCTGATATTGTGGGCAAGGCACTTCTTGCTCCTGCAAAACTCATTGCTAGTAATGCAGGGGATGATGGAGTAGTTGTTGTGGAGAAAACTCGAGCATGCGATTGGCGACACGGGTATAATGCAATGACAGACAAATATGAAGATCTTTTCAATGCTGGTGTAGTAGATCCTTGCCTTGTTTCCAGATGTGCGCTTCAGATTGCAGCCTCAGTTACCGGAATCGTTTTAACCACTCAAGCTGTAATGGTAGAGAAAATAAAGAAGCCAAAGCCACTCGTTCCCCATGTTCCTGGAATATCTCCATGA

Protein sequence

MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP*
Homology
BLAST of CsGy1G032850 vs. ExPASy Swiss-Prot
Match: Q56XV8 (Chaperonin 60 subunit alpha 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60A2 PE=2 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 3.3e-224
Identity = 421/579 (72.71%), Postives = 484/579 (83.59%), Query Frame = 0

Query: 28  SPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNF-VVRAGPKRISFGKECRGALLAGID 87
           SP+ F  T + P        R SG        R F VVRAG KRI +GK+ R  L AGID
Sbjct: 5   SPSSFSPTTISP--------RRSGQRNEP---RKFSVVRAGAKRILYGKDSREKLQAGID 64

Query: 88  KLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQEVASKMN 147
           KLADAVS+TLGP+GRNV+L+E+ T+KV+NDGVTIAK+IEL D IENAG  LIQEVA KMN
Sbjct: 65  KLADAVSITLGPRGRNVVLAEKDTIKVINDGVTIAKSIELPDTIENAGATLIQEVAIKMN 124

Query: 148 DLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKSTPVQGKD 207
           + AGDGTTTAIILAREMIK+G LA++FGA+ VS+K GM+KTVKEL++VL+ KS PVQGK+
Sbjct: 125 ESAGDGTTTAIILAREMIKAGSLAIAFGANAVSVKNGMNKTVKELVRVLQMKSIPVQGKN 184

Query: 208 DIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKIDKGYMSP 267
           DIKAVA IS+GNDE+VGNLIAE +EKIGPDGVISIESS +SETSVI+EEGMK DKGYMSP
Sbjct: 185 DIKAVASISAGNDEFVGNLIAETVEKIGPDGVISIESSSTSETSVIVEEGMKFDKGYMSP 244

Query: 268 QFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDISIKVLETL 327
            FITNQ+KS VEFD AK+LVTDQ+I+S KE+VPLLEKT QLS+PLLI AEDIS +VLE L
Sbjct: 245 HFITNQEKSTVEFDKAKILVTDQKITSAKELVPLLEKTSQLSVPLLIIAEDISAEVLEIL 304

Query: 328 VVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSDQLGIARK 387
           VVNK QGL+NVAVVKCPG+ + KKALLQDIALMTGAD+LSGDLG+ L GATSDQLG++R+
Sbjct: 305 VVNKKQGLINVAVVKCPGMLDGKKALLQDIALMTGADYLSGDLGMSLMGATSDQLGVSRR 364

Query: 388 VVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGGVAVIKVG 447
           VVIT+NSTTIVAD STK EIQARI+Q+KKDL ETDN  LS+K++ERIAKL+GGVAVIKVG
Sbjct: 365 VVITANSTTIVADASTKPEIQARIAQMKKDLAETDNSYLSKKIAERIAKLTGGVAVIKVG 424

Query: 448 AHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQS-MEDQDELIG 507
            HTE ELEDRKLRIEDAKNA FAAM EGIVPGGGATY+HL + +P IK++ MED  E IG
Sbjct: 425 GHTETELEDRKLRIEDAKNATFAAMREGIVPGGGATYIHLLDEIPRIKKNLMEDSYEQIG 484

Query: 508 ADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGVVDPCLV 567
           ADIV  AL APA  IA+NAG DG VVV+KTR  +WR GYNAM+ KYEDL NAG+ DPC V
Sbjct: 485 ADIVAMALTAPAMAIATNAGVDGSVVVQKTRELEWRSGYNAMSGKYEDLLNAGIADPCRV 544

Query: 568 SRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGI 605
           SR ALQ A SV GI+LTTQAV+VEKIK+PKP VP VPGI
Sbjct: 545 SRFALQNAVSVAGIILTTQAVLVEKIKQPKPAVPQVPGI 572

BLAST of CsGy1G032850 vs. ExPASy Swiss-Prot
Match: P21238 (Chaperonin 60 subunit alpha 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60A1 PE=1 SV=2)

HSP 1 Score: 683.3 bits (1762), Expect = 2.5e-195
Identity = 358/556 (64.39%), Postives = 454/556 (81.65%), Query Frame = 0

Query: 44  GGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNV 103
           G  Q+    + N   IR F VRA  K I+F +  R AL AGIDKLAD V +TLGP+GRNV
Sbjct: 24  GNQQQGQRVSYNKRTIRRFSVRANVKEIAFDQHSRAALQAGIDKLADCVGLTLGPRGRNV 83

Query: 104 ILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREM 163
           +L E  + KVVNDGVTIA+AIEL +A+ENAG  LI+EVASK ND AGDGTTTA ILARE+
Sbjct: 84  VLDEFGSPKVVNDGVTIARAIELPNAMENAGAALIREVASKTNDSAGDGTTTASILAREI 143

Query: 164 IKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVG 223
           IK GLL+V+ GA+PVSLK+G+DKTV+ LI+ L+KK+ PV+G+DDI+AVA IS+GND+ +G
Sbjct: 144 IKHGLLSVTSGANPVSLKRGIDKTVQGLIEELQKKARPVKGRDDIRAVASISAGNDDLIG 203

Query: 224 NLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAK 283
           ++IA+AI+K+GPDGV+SIESS S ET+V +EEGM+ID+GY+SPQF+TN +K + EF+NA+
Sbjct: 204 SMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMEIDRGYISPQFVTNPEKLLAEFENAR 263

Query: 284 VLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCP 343
           VL+TDQ+I+++K+I+P+LEKT QL  PLLI AED++ + L TLVVNK++G++NV  VK P
Sbjct: 264 VLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGVLNVVAVKAP 323

Query: 344 GVGERKKALLQDIALMTGADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTK 403
           G GER+KA+LQDIA++TGA++L+ D+ L +E AT DQLGIARKV I+ +STT++AD ++K
Sbjct: 324 GFGERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQLGIARKVTISKDSTTLIADAASK 383

Query: 404 DEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDA 463
           DE+QARI+Q+KK+L ETD+   S KL+ERIAKLSGGVAVIKVGA TE ELEDRKLRIEDA
Sbjct: 384 DELQARIAQLKKELFETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDA 443

Query: 464 KNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASN 523
           KNA FAA+ EGIVPGGGA  VHL  ++P IK++ ED DE +GADIV KALL+PA LIA N
Sbjct: 444 KNATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDADERLGADIVQKALLSPAALIAQN 503

Query: 524 AGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTT 583
           AG +G VVVEK    DW +GYNAMTD YE+LF AGV+DP  V+RCALQ AASV G+VLTT
Sbjct: 504 AGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVIDPAKVTRCALQNAASVAGMVLTT 563

Query: 584 QAVMVEKIKKPKPLVP 600
           QA++V+   KPKP  P
Sbjct: 564 QAIVVD---KPKPKAP 576

BLAST of CsGy1G032850 vs. ExPASy Swiss-Prot
Match: P08926 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 675.2 bits (1741), Expect = 6.7e-193
Identity = 367/587 (62.52%), Postives = 459/587 (78.19%), Query Frame = 0

Query: 16  ANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGK 75
           + N +  ++ L SP    QT L   +K  G        R   N   FVV+A  K I+F +
Sbjct: 3   STNALSSTSILRSPTNQAQTSLSKKVKQHGRVNF----RQKPN--RFVVKAAAKDIAFDQ 62

Query: 76  ECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGV 135
             R A+ AGIDKLADAV +TLGP+GRNV+L E  + KVVNDGVTIA+AIEL D +ENAG 
Sbjct: 63  HSRSAMQAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGA 122

Query: 136 VLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVL 195
            LI+EVASK ND AGDGTTTA ILARE+IK GLL V+ GA+PVS+KKG+DKTV  L++ L
Sbjct: 123 ALIREVASKTNDSAGDGTTTASILAREIIKLGLLNVTSGANPVSIKKGIDKTVAALVEEL 182

Query: 196 KKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEE 255
           +K + PV+G DDIKAVA IS+GNDE +G +IAEAI+K+GPDGV+SIESS S ET+V +EE
Sbjct: 183 EKLARPVKGGDDIKAVATISAGNDELIGKMIAEAIDKVGPDGVLSIESSNSFETTVEVEE 242

Query: 256 GMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFA 315
           GM+ID+GY+SPQF+TN +KSIVEF+NA+VL+TDQ+IS++K+I+PLLEKT QL  PLLI +
Sbjct: 243 GMEIDRGYISPQFVTNPEKSIVEFENARVLITDQKISAIKDIIPLLEKTTQLRAPLLIIS 302

Query: 316 EDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEG 375
           EDI+ + L TLVVNK++G++NVA +K PG GER+KALLQDIA++TGA+F + DLGL +E 
Sbjct: 303 EDITGEALATLVVNKLRGILNVAAIKAPGFGERRKALLQDIAILTGAEFQASDLGLLVEN 362

Query: 376 ATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAK 435
            T +QLG+ARKV I+ +STTI+AD ++KDE+Q+R++Q+KK+L ETD+   S KL+ERIAK
Sbjct: 363 TTIEQLGLARKVTISKDSTTIIADAASKDELQSRVAQLKKELSETDSIYDSEKLAERIAK 422

Query: 436 LSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQ 495
           LSGGVAVIKVGA TE ELEDRKLRIEDAKNA FAA+ EGIVPGGG   VHL   +P IK+
Sbjct: 423 LSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGTALVHLSGYVPAIKE 482

Query: 496 SMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLF 555
            +ED DE +GADIV KAL+APA LIA NAG +G VVVEK +  +W  GYNAMTD YE+L 
Sbjct: 483 KLEDADERLGADIVQKALVAPAALIAQNAGIEGEVVVEKIKNGEWEVGYNAMTDTYENLV 542

Query: 556 NAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVP 603
            +GV+DP  V+RCALQ AASV G+VLTTQA++VEK  KPK  V   P
Sbjct: 543 ESGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEK-PKPKAAVAAAP 582

BLAST of CsGy1G032850 vs. ExPASy Swiss-Prot
Match: P21239 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS=Brassica napus OX=3708 PE=2 SV=2)

HSP 1 Score: 674.9 bits (1740), Expect = 8.7e-193
Identity = 355/538 (65.99%), Postives = 444/538 (82.53%), Query Frame = 0

Query: 62  FVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIA 121
           F VRA  K ISF +  R AL AGIDKLADAV +TLGP+GRNV+L E  + KVVNDGVTIA
Sbjct: 2   FSVRANVKEISFDQSSRAALQAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIA 61

Query: 122 KAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLK 181
           +AIEL DA+ENAG  LI+EVASK ND AGDGTTTA +LARE+IK GLL+V+ GA+PVSLK
Sbjct: 62  RAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKHGLLSVTSGANPVSLK 121

Query: 182 KGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISI 241
           +G+DKTV+ LI+ L+K++ PV+G  DIKAVA IS+GNDE VG +IA+AI+K+GPDGV+SI
Sbjct: 122 RGIDKTVQALIEELEKRARPVKGGSDIKAVATISAGNDELVGTMIADAIDKVGPDGVLSI 181

Query: 242 ESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLL 301
           ESS S ET+V +EEGM+ID+GY+SPQF+TN +K +VEF+NA+VL+TDQ+I+++K+I+P+L
Sbjct: 182 ESSSSFETTVEVEEGMEIDRGYISPQFVTNPEKLLVEFENARVLITDQKITAIKDIIPIL 241

Query: 302 EKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTG 361
           EKT QL  PLLI AED++ + L TLVVNK++G++NV  VK PG GER+KA+LQDIA++TG
Sbjct: 242 EKTTQLRAPLLIIAEDVTGEALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTG 301

Query: 362 ADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETD 421
           A++ + D+GL +E  T DQLGIARKV I+ +STT++AD ++KDE+QARISQ+KK+L ETD
Sbjct: 302 AEYQALDMGLLVENTTIDQLGIARKVTISKDSTTLIADAASKDELQARISQLKKELSETD 361

Query: 422 NPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGA 481
           +   S KL+ERIAKL+GGVAVIKVGA TE ELEDRKLRIEDAKNA FAA+ EGIVPGGGA
Sbjct: 362 SVYDSEKLAERIAKLAGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGA 421

Query: 482 TYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWR 541
           T VHL  ++P IK+ +ED DE +GADIV KAL+APA LIA NAG +G VVVEK    +W 
Sbjct: 422 TLVHLSTVIPAIKEKLEDADERLGADIVQKALVAPAALIAQNAGIEGEVVVEKIMFSEWE 481

Query: 542 HGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVP 600
            GYNAMTD YE+L  AGV+DP  V+RCALQ AASV G+VLTTQA++V+   KPKP  P
Sbjct: 482 IGYNAMTDTYENLLEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVD---KPKPKAP 536

BLAST of CsGy1G032850 vs. ExPASy Swiss-Prot
Match: P08823 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 6.3e-191
Identity = 348/533 (65.29%), Postives = 444/533 (83.30%), Query Frame = 0

Query: 66  AGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIE 125
           A  K I+F ++ R AL AG++KLA+AV VTLGP+GRNV+L E    KVVNDGVTIA+AIE
Sbjct: 2   ADAKEIAFDQKSRAALQAGVEKLANAVGVTLGPRGRNVVLDEYGNPKVVNDGVTIARAIE 61

Query: 126 LSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMD 185
           L++ +ENAG  LI+EVASK ND AGDGTTTA +LARE+IK G+L+V+ GA+PVSLKKG+D
Sbjct: 62  LANPMENAGAALIREVASKTNDSAGDGTTTACVLAREIIKLGILSVTSGANPVSLKKGID 121

Query: 186 KTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSK 245
           KTV+ LI+ L++K+ PV+G  DIKAVA IS+GNDE +G +IA+AI+K+GPDGV+SIESS 
Sbjct: 122 KTVQGLIEELERKARPVKGSGDIKAVASISAGNDELIGAMIADAIDKVGPDGVLSIESSS 181

Query: 246 SSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTV 305
           S ET+V +EEGM+ID+GY+SPQF+TN +KSIVEF+NA+VL+TDQ+I+S+KEI+PLLE+T 
Sbjct: 182 SFETTVDVEEGMEIDRGYISPQFVTNLEKSIVEFENARVLITDQKITSIKEIIPLLEQTT 241

Query: 306 QLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFL 365
           QL  PL I AEDI+ + L TLVVNK++G++NVA +K P  GER+KA+LQDIA++TGA++L
Sbjct: 242 QLRCPLFIVAEDITGEALATLVVNKLRGIINVAAIKAPSFGERRKAVLQDIAIVTGAEYL 301

Query: 366 SGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNL 425
           + DLGL +E AT DQLG ARK+ I   +TT++AD ++KDEIQAR++Q+KK+L ETD+   
Sbjct: 302 AKDLGLLVENATVDQLGTARKITIHQTTTTLIADAASKDEIQARVAQLKKELSETDSIYD 361

Query: 426 SRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVH 485
           S KL+ERIAKLSGGVAVIKVGA TE ELEDR+LRIEDAKNA FAA+ EGIVPGGGA YVH
Sbjct: 362 SEKLAERIAKLSGGVAVIKVGATTETELEDRQLRIEDAKNATFAAIEEGIVPGGGAAYVH 421

Query: 486 LYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYN 545
           L   +P IK+++ED DE +GADI+ KAL APA LIA+NAG +G VV+EK +  +W  GYN
Sbjct: 422 LSTYVPAIKETIEDHDERLGADIIQKALQAPASLIANNAGVEGEVVIEKIKESEWEMGYN 481

Query: 546 AMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLV 599
           AMTDKYE+L  +GV+DP  V+RCALQ AASV+G+VLTTQA++VEK  KPKP V
Sbjct: 482 AMTDKYENLIESGVIDPAKVTRCALQNAASVSGMVLTTQAIVVEK-PKPKPKV 533

BLAST of CsGy1G032850 vs. NCBI nr
Match: XP_004145436.2 (chaperonin 60 subunit alpha 2, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 1145 bits (2961), Expect = 0.0
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0

Query: 1   MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIR 60
           MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIR
Sbjct: 1   MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIR 60

Query: 61  NFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTI 120
           NFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTI
Sbjct: 61  NFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTI 120

Query: 121 AKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSL 180
           AKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSL
Sbjct: 121 AKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSL 180

Query: 181 KKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVIS 240
           KKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVIS
Sbjct: 181 KKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVIS 240

Query: 241 IESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPL 300
           IESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPL
Sbjct: 241 IESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPL 300

Query: 301 LEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMT 360
           LEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMT
Sbjct: 301 LEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMT 360

Query: 361 GADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVET 420
           GADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVET
Sbjct: 361 GADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVET 420

Query: 421 DNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGG 480
           DNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGG
Sbjct: 421 DNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGG 480

Query: 481 ATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDW 540
           ATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDW
Sbjct: 481 ATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDW 540

Query: 541 RHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPH 600
           RHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPH
Sbjct: 541 RHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPH 600

Query: 601 VPGISP 606
           VPGISP
Sbjct: 601 VPGISP 606

BLAST of CsGy1G032850 vs. NCBI nr
Match: XP_008459023.1 (PREDICTED: chaperonin 60 subunit alpha 2, chloroplastic [Cucumis melo] >KAA0043182.1 chaperonin 60 subunit alpha 2 [Cucumis melo var. makuwa] >TYK12283.1 chaperonin 60 subunit alpha 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 564/587 (96.08%), Postives = 577/587 (98.30%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           MEFSARLSSPAPFPQTLLFPLMKHGG++RLSGYARNSWNIRNFVVRAGPKRISFGKECRG
Sbjct: 1   MEFSARLSSPAPFPQTLLFPLMKHGGNRRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSEQ TLKVVNDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEQGTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPV+GKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI
Sbjct: 181 TPVRGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NKIQGLVNVAVVKCPG+GERKKALLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKIQGLVNVAVVKCPGLGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDL+ETDN NLSRKLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLLETDNSNLSRKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIV GGGATYVHLYELLPTIKQSME+
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVAGGGATYVHLYELLPTIKQSMEN 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAPAKLIASNAGDDG+VVVEKT+ACDWRHGYNAM +KYEDLFNAGV
Sbjct: 481 QDEQIGADIVGKALLAPAKLIASNAGDDGIVVVEKTQACDWRHGYNAMANKYEDLFNAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           VDPCLVSRCALQIAASVTGIVLTTQA+MVEK KKPKP +P VPGISP
Sbjct: 541 VDPCLVSRCALQIAASVTGIVLTTQAIMVEKTKKPKPPIPLVPGISP 587

BLAST of CsGy1G032850 vs. NCBI nr
Match: XP_038894909.1 (chaperonin 60 subunit alpha 2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1035 bits (2675), Expect = 0.0
Identity = 549/587 (93.53%), Postives = 564/587 (96.08%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           ME  +RLSSP PF +TLLFP+ K GGSQRLSGYARNSW +RNFVVRAGPKRISFGKECRG
Sbjct: 1   MEVYSRLSSPPPFARTLLFPVTKLGGSQRLSGYARNSWKMRNFVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSEQ  LKV+NDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEQGMLKVINDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI
Sbjct: 181 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRIS+VKEIVPLLEKTVQLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISTVKEIVPLLEKTVQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NK+QGL+NVAVVKCPG+GERKKALLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKVQGLINVAVVKCPGLGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTK EIQARISQIKKDLVETDN  LS+KLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKAEIQARISQIKKDLVETDNSYLSKKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAM EGIVPGGGATYVHL ELLPTIKQSMED
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMTEGIVPGGGATYVHLSELLPTIKQSMED 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAP KLIASNAGDDGVVVVEKTRACDWRHGYNAM DKYEDLFNAGV
Sbjct: 481 QDEQIGADIVGKALLAPTKLIASNAGDDGVVVVEKTRACDWRHGYNAMADKYEDLFNAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           VDPCLVSRCALQIAAS+TGIVLTTQA+MVEK  KPKP +P VPGISP
Sbjct: 541 VDPCLVSRCALQIAASITGIVLTTQAIMVEKTNKPKPPIPLVPGISP 587

BLAST of CsGy1G032850 vs. NCBI nr
Match: XP_038894910.1 (chaperonin 60 subunit alpha 2, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 549/587 (93.53%), Postives = 563/587 (95.91%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           ME  +RLSSP PF +TLLFP  K GGSQRLSGYARNSW +RNFVVRAGPKRISFGKECRG
Sbjct: 1   MEVYSRLSSPPPFARTLLFP--KLGGSQRLSGYARNSWKMRNFVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSEQ  LKV+NDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEQGMLKVINDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI
Sbjct: 181 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRIS+VKEIVPLLEKTVQLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISTVKEIVPLLEKTVQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NK+QGL+NVAVVKCPG+GERKKALLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKVQGLINVAVVKCPGLGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTK EIQARISQIKKDLVETDN  LS+KLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKAEIQARISQIKKDLVETDNSYLSKKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAM EGIVPGGGATYVHL ELLPTIKQSMED
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMTEGIVPGGGATYVHLSELLPTIKQSMED 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAP KLIASNAGDDGVVVVEKTRACDWRHGYNAM DKYEDLFNAGV
Sbjct: 481 QDEQIGADIVGKALLAPTKLIASNAGDDGVVVVEKTRACDWRHGYNAMADKYEDLFNAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           VDPCLVSRCALQIAAS+TGIVLTTQA+MVEK  KPKP +P VPGISP
Sbjct: 541 VDPCLVSRCALQIAASITGIVLTTQAIMVEKTNKPKPPIPLVPGISP 585

BLAST of CsGy1G032850 vs. NCBI nr
Match: XP_022927052.1 (chaperonin 60 subunit alpha 2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 539/587 (91.82%), Postives = 558/587 (95.06%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           ME  ARLSSPA  P+TLLFP  K GGSQRL  YARNSW +RN VVRAGPKRISFGKECRG
Sbjct: 1   MEVPARLSSPAAVPRTLLFPATKLGGSQRLFRYARNSWKMRNLVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSE++TLKVVNDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEKETLKVVNDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELI+VLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIEVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPVQGK+DIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSET VIIEEGMKI
Sbjct: 181 TPVQGKNDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETCVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQ IS+VKEIVPLLEKT+QLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQIISTVKEIVPLLEKTIQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NK+QGLVNVAVVKCPG+GERKK+LLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKVQGLVNVAVVKCPGLGERKKSLLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTK EIQARISQIKKDLVETDN  LSRKLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKAEIQARISQIKKDLVETDNSYLSRKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHL E LP+IK SMED
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLSEFLPSIKHSMED 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAPAK IASNAG DGVVVVEKTRAC WRHGYNAM D+YEDL NAGV
Sbjct: 481 QDEQIGADIVGKALLAPAKFIASNAGVDGVVVVEKTRACHWRHGYNAMADRYEDLINAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           +DPCLVSRCALQIAASV GI+LTTQA+MV+KIKKPKP VPHVPGISP
Sbjct: 541 LDPCLVSRCALQIAASVAGIILTTQAIMVDKIKKPKPPVPHVPGISP 587

BLAST of CsGy1G032850 vs. ExPASy TrEMBL
Match: A0A0A0LYB3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701900 PE=3 SV=1)

HSP 1 Score: 1145 bits (2961), Expect = 0.0
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0

Query: 1   MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIR 60
           MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIR
Sbjct: 1   MNLNYPLISSSLLYDANNLMEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIR 60

Query: 61  NFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTI 120
           NFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTI
Sbjct: 61  NFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTI 120

Query: 121 AKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSL 180
           AKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSL
Sbjct: 121 AKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSL 180

Query: 181 KKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVIS 240
           KKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVIS
Sbjct: 181 KKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVIS 240

Query: 241 IESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPL 300
           IESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPL
Sbjct: 241 IESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPL 300

Query: 301 LEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMT 360
           LEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMT
Sbjct: 301 LEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMT 360

Query: 361 GADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVET 420
           GADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVET
Sbjct: 361 GADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVET 420

Query: 421 DNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGG 480
           DNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGG
Sbjct: 421 DNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGG 480

Query: 481 ATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDW 540
           ATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDW
Sbjct: 481 ATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDW 540

Query: 541 RHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPH 600
           RHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPH
Sbjct: 541 RHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPH 600

Query: 601 VPGISP 606
           VPGISP
Sbjct: 601 VPGISP 606

BLAST of CsGy1G032850 vs. ExPASy TrEMBL
Match: A0A5A7TIP5 (Chaperonin 60 subunit alpha 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00470 PE=3 SV=1)

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 564/587 (96.08%), Postives = 577/587 (98.30%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           MEFSARLSSPAPFPQTLLFPLMKHGG++RLSGYARNSWNIRNFVVRAGPKRISFGKECRG
Sbjct: 1   MEFSARLSSPAPFPQTLLFPLMKHGGNRRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSEQ TLKVVNDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEQGTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPV+GKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI
Sbjct: 181 TPVRGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NKIQGLVNVAVVKCPG+GERKKALLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKIQGLVNVAVVKCPGLGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDL+ETDN NLSRKLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLLETDNSNLSRKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIV GGGATYVHLYELLPTIKQSME+
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVAGGGATYVHLYELLPTIKQSMEN 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAPAKLIASNAGDDG+VVVEKT+ACDWRHGYNAM +KYEDLFNAGV
Sbjct: 481 QDEQIGADIVGKALLAPAKLIASNAGDDGIVVVEKTQACDWRHGYNAMANKYEDLFNAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           VDPCLVSRCALQIAASVTGIVLTTQA+MVEK KKPKP +P VPGISP
Sbjct: 541 VDPCLVSRCALQIAASVTGIVLTTQAIMVEKTKKPKPPIPLVPGISP 587

BLAST of CsGy1G032850 vs. ExPASy TrEMBL
Match: A0A1S3C9C2 (chaperonin 60 subunit alpha 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498255 PE=3 SV=1)

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 564/587 (96.08%), Postives = 577/587 (98.30%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           MEFSARLSSPAPFPQTLLFPLMKHGG++RLSGYARNSWNIRNFVVRAGPKRISFGKECRG
Sbjct: 1   MEFSARLSSPAPFPQTLLFPLMKHGGNRRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSEQ TLKVVNDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEQGTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPV+GKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI
Sbjct: 181 TPVRGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NKIQGLVNVAVVKCPG+GERKKALLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKIQGLVNVAVVKCPGLGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDL+ETDN NLSRKLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLLETDNSNLSRKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIV GGGATYVHLYELLPTIKQSME+
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVAGGGATYVHLYELLPTIKQSMEN 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAPAKLIASNAGDDG+VVVEKT+ACDWRHGYNAM +KYEDLFNAGV
Sbjct: 481 QDEQIGADIVGKALLAPAKLIASNAGDDGIVVVEKTQACDWRHGYNAMANKYEDLFNAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           VDPCLVSRCALQIAASVTGIVLTTQA+MVEK KKPKP +P VPGISP
Sbjct: 541 VDPCLVSRCALQIAASVTGIVLTTQAIMVEKTKKPKPPIPLVPGISP 587

BLAST of CsGy1G032850 vs. ExPASy TrEMBL
Match: A0A6J1EG21 (chaperonin 60 subunit alpha 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433995 PE=3 SV=1)

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 539/587 (91.82%), Postives = 558/587 (95.06%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           ME  ARLSSPA  P+TLLFP  K GGSQRL  YARNSW +RN VVRAGPKRISFGKECRG
Sbjct: 1   MEVPARLSSPAAVPRTLLFPATKLGGSQRLFRYARNSWKMRNLVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSE++TLKVVNDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEKETLKVVNDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELI+VLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIEVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPVQGK+DIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSET VIIEEGMKI
Sbjct: 181 TPVQGKNDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETCVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQFITNQDKSIVEFDNAKVLVTDQ IS+VKEIVPLLEKT+QLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQIISTVKEIVPLLEKTIQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NK+QGLVNVAVVKCPG+GERKK+LLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKVQGLVNVAVVKCPGLGERKKSLLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTK EIQARISQIKKDLVETDN  LSRKLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKAEIQARISQIKKDLVETDNSYLSRKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHL E LP+IK SMED
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLSEFLPSIKHSMED 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           QDE IGADIVGKALLAPAK IASNAG DGVVVVEKTRAC WRHGYNAM D+YEDL NAGV
Sbjct: 481 QDEQIGADIVGKALLAPAKFIASNAGVDGVVVVEKTRACHWRHGYNAMADRYEDLINAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           +DPCLVSRCALQIAASV GI+LTTQA+MV+KIKKPKP VPHVPGISP
Sbjct: 541 LDPCLVSRCALQIAASVAGIILTTQAIMVDKIKKPKPPVPHVPGISP 587

BLAST of CsGy1G032850 vs. ExPASy TrEMBL
Match: A0A6J1KN21 (chaperonin 60 subunit alpha 2, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495653 PE=3 SV=1)

HSP 1 Score: 1011 bits (2614), Expect = 0.0
Identity = 536/587 (91.31%), Postives = 558/587 (95.06%), Query Frame = 0

Query: 20  MEFSARLSSPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRG 79
           ME  ARLS+PAP P+TLLFP+ K GGSQRL  YARNSW +RN VVRAGPKRISFGKECRG
Sbjct: 1   MEVPARLSAPAPVPRTLLFPVTKLGGSQRLFRYARNSWKMRNLVVRAGPKRISFGKECRG 60

Query: 80  ALLAGIDKLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 139
           ALLAGIDKLADAVSVTLGPKGRNVILSE++TLKVVNDGVTIAKAIELSDAIENAGVVLIQ
Sbjct: 61  ALLAGIDKLADAVSVTLGPKGRNVILSEKETLKVVNDGVTIAKAIELSDAIENAGVVLIQ 120

Query: 140 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 199
           EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMD TVKELI+VLKKKS
Sbjct: 121 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDTTVKELIEVLKKKS 180

Query: 200 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 259
           TPVQGK+DIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSET VIIEEGMKI
Sbjct: 181 TPVQGKNDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETCVIIEEGMKI 240

Query: 260 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 319
           DKGYMSPQF+TNQDKSIVEFDNAKVLVTDQ IS+VKEI+PLLEKTVQLSLPLLI AEDIS
Sbjct: 241 DKGYMSPQFVTNQDKSIVEFDNAKVLVTDQIISTVKEIIPLLEKTVQLSLPLLIIAEDIS 300

Query: 320 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 379
            +VLETLV+NK+QGLVNVAVVKCPG+GERKK+LLQDIALMTGADFLSGDLGLGLEGATSD
Sbjct: 301 RQVLETLVLNKVQGLVNVAVVKCPGLGERKKSLLQDIALMTGADFLSGDLGLGLEGATSD 360

Query: 380 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 439
           QLGIARKVVITSNSTTIVADPSTK EIQARISQIKKDLVETDN  LSRKLSERIAKLSGG
Sbjct: 361 QLGIARKVVITSNSTTIVADPSTKAEIQARISQIKKDLVETDNSYLSRKLSERIAKLSGG 420

Query: 440 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 499
           VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHL E LPTIK SMED
Sbjct: 421 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLSEFLPTIKHSMED 480

Query: 500 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGV 559
           +DE IGADIVGKALLAP K IASNAG DGVVVVEKTRAC WRHGYNAM D+YEDL NAGV
Sbjct: 481 RDEQIGADIVGKALLAPTKFIASNAGVDGVVVVEKTRACHWRHGYNAMADRYEDLINAGV 540

Query: 560 VDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGISP 606
           +DPCLVSRCALQIAASV GI+LTTQA+MV+KIKKPKP VPHVPGISP
Sbjct: 541 LDPCLVSRCALQIAASVAGIILTTQAIMVDKIKKPKPPVPHVPGISP 587

BLAST of CsGy1G032850 vs. TAIR 10
Match: AT5G18820.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 779.2 bits (2011), Expect = 2.3e-225
Identity = 421/579 (72.71%), Postives = 484/579 (83.59%), Query Frame = 0

Query: 28  SPAPFPQTLLFPLMKHGGSQRLSGYARNSWNIRNF-VVRAGPKRISFGKECRGALLAGID 87
           SP+ F  T + P        R SG        R F VVRAG KRI +GK+ R  L AGID
Sbjct: 5   SPSSFSPTTISP--------RRSGQRNEP---RKFSVVRAGAKRILYGKDSREKLQAGID 64

Query: 88  KLADAVSVTLGPKGRNVILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQEVASKMN 147
           KLADAVS+TLGP+GRNV+L+E+ T+KV+NDGVTIAK+IEL D IENAG  LIQEVA KMN
Sbjct: 65  KLADAVSITLGPRGRNVVLAEKDTIKVINDGVTIAKSIELPDTIENAGATLIQEVAIKMN 124

Query: 148 DLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKSTPVQGKD 207
           + AGDGTTTAIILAREMIK+G LA++FGA+ VS+K GM+KTVKEL++VL+ KS PVQGK+
Sbjct: 125 ESAGDGTTTAIILAREMIKAGSLAIAFGANAVSVKNGMNKTVKELVRVLQMKSIPVQGKN 184

Query: 208 DIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKIDKGYMSP 267
           DIKAVA IS+GNDE+VGNLIAE +EKIGPDGVISIESS +SETSVI+EEGMK DKGYMSP
Sbjct: 185 DIKAVASISAGNDEFVGNLIAETVEKIGPDGVISIESSSTSETSVIVEEGMKFDKGYMSP 244

Query: 268 QFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDISIKVLETL 327
            FITNQ+KS VEFD AK+LVTDQ+I+S KE+VPLLEKT QLS+PLLI AEDIS +VLE L
Sbjct: 245 HFITNQEKSTVEFDKAKILVTDQKITSAKELVPLLEKTSQLSVPLLIIAEDISAEVLEIL 304

Query: 328 VVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSDQLGIARK 387
           VVNK QGL+NVAVVKCPG+ + KKALLQDIALMTGAD+LSGDLG+ L GATSDQLG++R+
Sbjct: 305 VVNKKQGLINVAVVKCPGMLDGKKALLQDIALMTGADYLSGDLGMSLMGATSDQLGVSRR 364

Query: 388 VVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGGVAVIKVG 447
           VVIT+NSTTIVAD STK EIQARI+Q+KKDL ETDN  LS+K++ERIAKL+GGVAVIKVG
Sbjct: 365 VVITANSTTIVADASTKPEIQARIAQMKKDLAETDNSYLSKKIAERIAKLTGGVAVIKVG 424

Query: 448 AHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQS-MEDQDELIG 507
            HTE ELEDRKLRIEDAKNA FAAM EGIVPGGGATY+HL + +P IK++ MED  E IG
Sbjct: 425 GHTETELEDRKLRIEDAKNATFAAMREGIVPGGGATYIHLLDEIPRIKKNLMEDSYEQIG 484

Query: 508 ADIVGKALLAPAKLIASNAGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGVVDPCLV 567
           ADIV  AL APA  IA+NAG DG VVV+KTR  +WR GYNAM+ KYEDL NAG+ DPC V
Sbjct: 485 ADIVAMALTAPAMAIATNAGVDGSVVVQKTRELEWRSGYNAMSGKYEDLLNAGIADPCRV 544

Query: 568 SRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVPHVPGI 605
           SR ALQ A SV GI+LTTQAV+VEKIK+PKP VP VPGI
Sbjct: 545 SRFALQNAVSVAGIILTTQAVLVEKIKQPKPAVPQVPGI 572

BLAST of CsGy1G032850 vs. TAIR 10
Match: AT2G28000.1 (chaperonin-60alpha )

HSP 1 Score: 683.3 bits (1762), Expect = 1.7e-196
Identity = 358/556 (64.39%), Postives = 454/556 (81.65%), Query Frame = 0

Query: 44  GGSQRLSGYARNSWNIRNFVVRAGPKRISFGKECRGALLAGIDKLADAVSVTLGPKGRNV 103
           G  Q+    + N   IR F VRA  K I+F +  R AL AGIDKLAD V +TLGP+GRNV
Sbjct: 24  GNQQQGQRVSYNKRTIRRFSVRANVKEIAFDQHSRAALQAGIDKLADCVGLTLGPRGRNV 83

Query: 104 ILSEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREM 163
           +L E  + KVVNDGVTIA+AIEL +A+ENAG  LI+EVASK ND AGDGTTTA ILARE+
Sbjct: 84  VLDEFGSPKVVNDGVTIARAIELPNAMENAGAALIREVASKTNDSAGDGTTTASILAREI 143

Query: 164 IKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVG 223
           IK GLL+V+ GA+PVSLK+G+DKTV+ LI+ L+KK+ PV+G+DDI+AVA IS+GND+ +G
Sbjct: 144 IKHGLLSVTSGANPVSLKRGIDKTVQGLIEELQKKARPVKGRDDIRAVASISAGNDDLIG 203

Query: 224 NLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAK 283
           ++IA+AI+K+GPDGV+SIESS S ET+V +EEGM+ID+GY+SPQF+TN +K + EF+NA+
Sbjct: 204 SMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMEIDRGYISPQFVTNPEKLLAEFENAR 263

Query: 284 VLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCP 343
           VL+TDQ+I+++K+I+P+LEKT QL  PLLI AED++ + L TLVVNK++G++NV  VK P
Sbjct: 264 VLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGVLNVVAVKAP 323

Query: 344 GVGERKKALLQDIALMTGADFLSGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTK 403
           G GER+KA+LQDIA++TGA++L+ D+ L +E AT DQLGIARKV I+ +STT++AD ++K
Sbjct: 324 GFGERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQLGIARKVTISKDSTTLIADAASK 383

Query: 404 DEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDA 463
           DE+QARI+Q+KK+L ETD+   S KL+ERIAKLSGGVAVIKVGA TE ELEDRKLRIEDA
Sbjct: 384 DELQARIAQLKKELFETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDA 443

Query: 464 KNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASN 523
           KNA FAA+ EGIVPGGGA  VHL  ++P IK++ ED DE +GADIV KALL+PA LIA N
Sbjct: 444 KNATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDADERLGADIVQKALLSPAALIAQN 503

Query: 524 AGDDGVVVVEKTRACDWRHGYNAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTT 583
           AG +G VVVEK    DW +GYNAMTD YE+LF AGV+DP  V+RCALQ AASV G+VLTT
Sbjct: 504 AGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVIDPAKVTRCALQNAASVAGMVLTT 563

Query: 584 QAVMVEKIKKPKPLVP 600
           QA++V+   KPKP  P
Sbjct: 564 QAIVVD---KPKPKAP 576

BLAST of CsGy1G032850 vs. TAIR 10
Match: AT1G55490.1 (chaperonin 60 beta )

HSP 1 Score: 470.3 bits (1209), Expect = 2.3e-132
Identity = 253/519 (48.75%), Postives = 369/519 (71.10%), Query Frame = 0

Query: 81  LLAGIDKLADAVSVTLGPKGRNVIL-SEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 140
           L AG++KLAD V VTLGPKGRNV+L S+  + ++VNDGVT+A+ +EL D +EN G  L++
Sbjct: 71  LQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVR 130

Query: 141 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 200
           + A+K NDLAGDGTTT+++LA+  I  G+  V+ GA+PV + +G++KT K L+  LKK S
Sbjct: 131 QAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMS 190

Query: 201 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 260
             V+   ++  VA +S+GN++ +GN+IAEA+ K+G  GV+++E  KS+E ++ + EGM+ 
Sbjct: 191 KEVE-DSELADVAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQF 250

Query: 261 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 320
           D+GY+SP F+T+ +K  VEFDN K+L+ D++I++ +++V +LE  ++   P+LI AEDI 
Sbjct: 251 DRGYISPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIE 310

Query: 321 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 380
            + L TLVVNK++G + +A ++ PG GERK   L DIA++TGA  +  ++GL L+ A  +
Sbjct: 311 QEALATLVVNKLRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKE 370

Query: 381 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 440
            LG A KVV+T  ++TIV D ST+D ++ R++QIK  + + +      KL+ERIAKLSGG
Sbjct: 371 VLGNASKVVLTKETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGG 430

Query: 441 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 500
           VAVI+VGA TE EL+++KLR+EDA NA  AA+ EGIV GGG T + L   +  IK ++++
Sbjct: 431 VAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDN 490

Query: 501 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACD-WRHGYNAMTDKYEDLFNAG 560
            +E +GADIV +AL  P KLIA NAG +G VV EK  + D  + GYNA T KYEDL  AG
Sbjct: 491 DEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAG 550

Query: 561 VVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPL 598
           ++DP  V RC L+ AASV    L +  V+VE IK+P+P+
Sbjct: 551 IIDPTKVVRCCLEHAASVAKTFLMSDCVVVE-IKEPEPV 587

BLAST of CsGy1G032850 vs. TAIR 10
Match: AT1G55490.2 (chaperonin 60 beta )

HSP 1 Score: 470.3 bits (1209), Expect = 2.3e-132
Identity = 253/519 (48.75%), Postives = 369/519 (71.10%), Query Frame = 0

Query: 81  LLAGIDKLADAVSVTLGPKGRNVIL-SEQKTLKVVNDGVTIAKAIELSDAIENAGVVLIQ 140
           L AG++KLAD V VTLGPKGRNV+L S+  + ++VNDGVT+A+ +EL D +EN G  L++
Sbjct: 71  LQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVR 130

Query: 141 EVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMDKTVKELIKVLKKKS 200
           + A+K NDLAGDGTTT+++LA+  I  G+  V+ GA+PV + +G++KT K L+  LKK S
Sbjct: 131 QAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMS 190

Query: 201 TPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSKSSETSVIIEEGMKI 260
             V+   ++  VA +S+GN++ +GN+IAEA+ K+G  GV+++E  KS+E ++ + EGM+ 
Sbjct: 191 KEVE-DSELADVAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQF 250

Query: 261 DKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTVQLSLPLLIFAEDIS 320
           D+GY+SP F+T+ +K  VEFDN K+L+ D++I++ +++V +LE  ++   P+LI AEDI 
Sbjct: 251 DRGYISPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIE 310

Query: 321 IKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFLSGDLGLGLEGATSD 380
            + L TLVVNK++G + +A ++ PG GERK   L DIA++TGA  +  ++GL L+ A  +
Sbjct: 311 QEALATLVVNKLRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKE 370

Query: 381 QLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNLSRKLSERIAKLSGG 440
            LG A KVV+T  ++TIV D ST+D ++ R++QIK  + + +      KL+ERIAKLSGG
Sbjct: 371 VLGNASKVVLTKETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGG 430

Query: 441 VAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVHLYELLPTIKQSMED 500
           VAVI+VGA TE EL+++KLR+EDA NA  AA+ EGIV GGG T + L   +  IK ++++
Sbjct: 431 VAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDN 490

Query: 501 QDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACD-WRHGYNAMTDKYEDLFNAG 560
            +E +GADIV +AL  P KLIA NAG +G VV EK  + D  + GYNA T KYEDL  AG
Sbjct: 491 DEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAG 550

Query: 561 VVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPL 598
           ++DP  V RC L+ AASV    L +  V+VE IK+P+P+
Sbjct: 551 IIDPTKVVRCCLEHAASVAKTFLMSDCVVVE-IKEPEPV 587

BLAST of CsGy1G032850 vs. TAIR 10
Match: AT5G56500.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 6.7e-132
Identity = 259/535 (48.41%), Postives = 370/535 (69.16%), Query Frame = 0

Query: 69  KRISFGKECRG--ALLAGIDKLADAVSVTLGPKGRNVIL-SEQKTLKVVNDGVTIAKAIE 128
           K++ F K+      L AG++KLAD V VTLGPKGRNV+L S+  + ++VNDGVT+A+ +E
Sbjct: 53  KQLHFNKDGTAIKKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVE 112

Query: 129 LSDAIENAGVVLIQEVASKMNDLAGDGTTTAIILAREMIKSGLLAVSFGADPVSLKKGMD 188
           L D +EN G  L+++ ASK NDLAGDGTTT+++LA+ +I  G+  V+ GA+PV + +G++
Sbjct: 113 LEDPVENIGAKLVRQAASKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITRGIE 172

Query: 189 KTVKELIKVLKKKSTPVQGKDDIKAVAMISSGNDEYVGNLIAEAIEKIGPDGVISIESSK 248
           KT K L+  LKK S  V+   ++  VA +S+GN+  VGN+IAEA+ K+G  GV+++E  K
Sbjct: 173 KTTKALVAELKKMSKEVE-DSELADVAAVSAGNNYEVGNMIAEAMAKVGRKGVVTLEEGK 232

Query: 249 SSETSVIIEEGMKIDKGYMSPQFITNQDKSIVEFDNAKVLVTDQRISSVKEIVPLLEKTV 308
           S+E S+ + EGM+ D+GY+SP F+T+ +K   E++N K+ + D++I++ ++I+ +LE  +
Sbjct: 233 SAENSLYVVEGMQFDRGYISPYFVTDSEKMCAEYENCKLFLVDKKITNARDIISILEDAI 292

Query: 309 QLSLPLLIFAEDISIKVLETLVVNKIQGLVNVAVVKCPGVGERKKALLQDIALMTGADFL 368
           +   PLLI AEDI  + L TLVVNK++G + VA +K PG GERK   L DIA +TGA  +
Sbjct: 293 KGGYPLLIIAEDIEQEPLATLVVNKLRGTIKVAALKAPGFGERKSQYLDDIAALTGATVI 352

Query: 369 SGDLGLGLEGATSDQLGIARKVVITSNSTTIVADPSTKDEIQARISQIKKDLVETDNPNL 428
             ++GL LE    + LG A KVV+T ++TTIV D ST++ ++ R+ QIK  +   +    
Sbjct: 353 REEVGLQLEKVGPEVLGNAGKVVLTKDTTTIVGDGSTEEVVKKRVEQIKNLIEAAEQDYE 412

Query: 429 SRKLSERIAKLSGGVAVIKVGAHTEVELEDRKLRIEDAKNAVFAAMNEGIVPGGGATYVH 488
             KL+ERIAKLSGGVAVI+VGA TE EL+++KLR+EDA NA  AA+ EGIV GGG T + 
Sbjct: 413 KEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLR 472

Query: 489 LYELLPTIKQSMEDQDELIGADIVGKALLAPAKLIASNAGDDGVVVVEKTRACD-WRHGY 548
           L   +  IK+++ + +E +GADIV KAL  P KLIA NAG +G VV EK  + D  +HGY
Sbjct: 473 LASKVDAIKETLANDEEKVGADIVKKALSYPLKLIAKNAGVNGSVVSEKVLSSDNPKHGY 532

Query: 549 NAMTDKYEDLFNAGVVDPCLVSRCALQIAASVTGIVLTTQAVMVEKIKKPKPLVP 600
           NA T KYEDL  AG++DP  V RC L+ A+SV    L +  V+VE IK+P+   P
Sbjct: 533 NAATGKYEDLMAAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVE-IKEPESAAP 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56XV83.3e-22472.71Chaperonin 60 subunit alpha 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P212382.5e-19564.39Chaperonin 60 subunit alpha 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P089266.7e-19362.52RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Pisum sati... [more]
P212398.7e-19365.99RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS... [more]
P088236.3e-19165.29RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS... [more]
Match NameE-valueIdentityDescription
XP_004145436.20.0100.00chaperonin 60 subunit alpha 2, chloroplastic isoform X1 [Cucumis sativus][more]
XP_008459023.10.096.08PREDICTED: chaperonin 60 subunit alpha 2, chloroplastic [Cucumis melo] >KAA00431... [more]
XP_038894909.10.093.53chaperonin 60 subunit alpha 2, chloroplastic isoform X1 [Benincasa hispida][more]
XP_038894910.10.093.53chaperonin 60 subunit alpha 2, chloroplastic isoform X2 [Benincasa hispida][more]
XP_022927052.10.091.82chaperonin 60 subunit alpha 2, chloroplastic isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0LYB30.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701900 PE=3 SV=1[more]
A0A5A7TIP50.096.08Chaperonin 60 subunit alpha 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3C9C20.096.08chaperonin 60 subunit alpha 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1EG210.091.82chaperonin 60 subunit alpha 2, chloroplastic isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1KN210.091.31chaperonin 60 subunit alpha 2, chloroplastic isoform X1 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
AT5G18820.12.3e-22572.71TCP-1/cpn60 chaperonin family protein [more]
AT2G28000.11.7e-19664.39chaperonin-60alpha [more]
AT1G55490.12.3e-13248.75chaperonin 60 beta [more]
AT1G55490.22.3e-13248.75chaperonin 60 beta [more]
AT5G56500.16.7e-13248.41TCP-1/cpn60 chaperonin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001844Chaperonin Cpn60PRINTSPR00298CHAPERONIN60coord: 332..355
score: 50.5
coord: 147..174
score: 53.7
coord: 414..439
score: 42.77
coord: 462..483
score: 58.03
coord: 92..118
score: 42.28
IPR001844Chaperonin Cpn60TIGRFAMTIGR02348TIGR02348coord: 68..591
e-value: 8.5E-191
score: 632.9
IPR001844Chaperonin Cpn60CDDcd03344GroELcoord: 69..589
e-value: 0.0
score: 708.454
IPR027413GroEL-like equatorial domain superfamilyGENE3D1.10.560.10coord: 71..587
e-value: 1.3E-190
score: 636.2
IPR027413GroEL-like equatorial domain superfamilySUPERFAMILY48592GroEL equatorial domain-likecoord: 76..587
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 88..590
e-value: 1.4E-89
score: 301.0
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 256..437
e-value: 1.3E-190
score: 636.2
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 249..440
IPR027410TCP-1-like chaperonin intermediate domain superfamilyGENE3D3.30.260.10coord: 201..474
e-value: 1.3E-190
score: 636.2
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 201..267
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 428..473
NoneNo IPR availablePANTHERPTHR4563360 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 54..605
NoneNo IPR availablePANTHERPTHR45633:SF13CHAPERONIN 60 SUBUNIT ALPHA 2, CHLOROPLASTICcoord: 54..605

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G032850.2CsGy1G032850.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042026 protein refolding
cellular_component GO:0009507 chloroplast
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity