CsGy1G016630 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G016630
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionsubtilisin-like protease SBT6.1
LocationGy14Chr1: 13894878 .. 13899034 (-)
RNA-Seq ExpressionCsGy1G016630
SyntenyCsGy1G016630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTATTAAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCATTTGTAGAGAAGGTAATTTTTCTTTGATTGGCTGATTGCAATTTGGTGATCTGGAAAAATAAGTTTTCAACTGAATACGACTTAATTCTTTTTTGGTTTGAGGTTAATTAATTGCTGAATGTCTGTTCAAATAAATATCTCCTACATGTGAGTTGGCTATTAAATAAAATTATTTACCTTCTTTTCAGGTTACAGTTAGCATTGCTATTACAAACATTAATGAAATCATTTAAATGTGTTATTGTTGGGGTACTGCTAGTTCTTTCTTCATGATATATGTTAGGTTAAATACAAAATTCATTCTGCGAACTTTTAAATCTTGTAAAGTTCATAAATCTATTAGACTCAAAATTTATATCTAATAGATCAATTAGTTTTTAAACGCTTTGACTTTGTTAAAAATCTATTACACTTTTAAAAGTTCAAAGTTTAGAGACTAACCCTGTAATTTAACCTATATGTTTACAATTATTGGACGACCTATATAGTCTTGCAGTTTTATTAATATTAAATTATAACATGATCATCGATGAACATGCGGTTGAAGACTTTCTCATTGAAAGAATCTCTTAATGAAGTTTCGTCAGAAGATTTATTAGCTTATTGAGTATTTGGTTTCTTATATTGTTGTATCAGTAGTTTAAACTTTTGGCTTTCAGGTTGTAACATATTAAAAAATTGAGGCCTTTGATCTGGGCATCAGAAAATTATTTCCAATTAATTGAATTTCTATGCATTAGACTTAACATAATCATGCGTGTCACCGCAGGCTAATGAGTGATATTATGGCATCATCATTGTATGTTAAACTTTTTATTTGTCTGCCAGATTTGGGAAATAACAGCAAATAATATCATCATGGTTTCTGCAATTGGAAATGATGGACCACTTTATGGGACTTTAAACAATCCTGCAGATCAGAGTGATGTCATTGGTGTTGGGGGTATTGACTACAATGATCATATTGCTTCATTTTCCTCACGTGGCATGACTACTTGGGAGATGCCTCATGGGTAGATTACACTTTGCTTAGACTCTTAAAGTTCCTTCAGTATATTTTACTCCTATCTCCTTCAGATATTTTGAGCAGTTTCAAAATGTTAAAGCTATTGCTTGCTTGGTAATATTGGCAATGATTTATTACCTTCCTTTCATTGGAATTTGGCCTTCTATGAAGCAGATGTGTTGGATGAATTGAAATTCAAACTTAAGCCCATAAAGTTTTGACTACTTGTCCAATATATAGATAAATGGGTTTATATCAATTAAGGCTCCAATTACGTCGCAAAGATCATGGTTTGATTCTCCACCCCCACAATTATTAAACTAAATTATTTTACCTTATACAGTTATACTACTGTACTTTCTTTTTTTCGTTCCCTCTTTCGGTCCATATTGACACTCTCAAACTTGTCATTTGTACAGTTATGGTCGTGTGAAGCCCGATGTTGTTGCATATGGTCGGGAAATTATGGGATCCAAAATTAGTACTGGATGCAAAAGTTTATCAGGCACTAGTGTCGCTAGTCCTGTTGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCTGAAAGCCAGCGGAAGGTTATTTTAAATCCAGCCAGCATGAAACAAGCACTTGTTGAGGGGGCTGCAAAGCTTGCTGGTCCTAATATGTATGAGCAAGGAGCTGGCAGAGTTGATCTGTAGGTTTAAACTCTCATTGTTGTTTTTCTTTATATGTTTTGCTTCTAGAGTGTATTCTTATTTCTCTGAATATTCTCAGGTTAGAATCGTATGAAGTACTTAAGAGCTACCAACCCAGGGCAAGTATCTTCCCTGGTGTTCTTGACTATACAGATTGCCCTTATACATGGCCCTTTTGCCGCCAACCTCTTTATGCAGGTGCGATGCCTATTATTTTCAATGCTACCATTTTGAATGGTATGGGTGTAATTGGCTATGTTGAAGGTCAACCAACTTGGCACCCTTCTGATGAAGAAGGCAATCTCTTAAGTATTCACTTCACTTATTCTAAGGTCATTTGGCCATGGACTGGTTATATTGCACTTCATATGCAAATTAAGGAAGAAGGTGCACAATTTTCTGGAGAAATTGAGGGCAATGTGACTCTAACAGTATATAGTCCTCCATCAAGAGGCGAGAAGAACCGCAGGATTAGTACTTGTGTCCTACAGCTGAAGCTAAAAGTAGTTCCTACACCTCCAAGATCAAAACGAATTTTATGGGATCAATTTCATAATATCAAATATCCTCCTGGTTATATTCCTAGAGATTCCTTGGATGTACGCAATGACATTCTGGACTGGCATGGAGACCACCTGCACACAAATTTTCATATCATGTTTAACATGTTAAGAGATGCTGGGTACTATGTTGAAACCCTTGGTTCTCCTCTTACATGCTTTGATGCTCGTCAATATGGAACACTTCTACTAGTGGATCTTGAAGACGAATACTTCAAAGAGGAAATTGAGAAGTTGAGGGACGATGTTATGACTACTGGATTGGGATTGGCTGTGTTTTCCGAATGGTATAATGTAGAAACAATGGTAAAGATGAGATTCTTTGATGATAACACAAGAAGTTGGTGGACACCAGTTACTGGAGGTGCAAATATTCCTGCACTCAATGATCTCTTAGCCCCATTTGGCATTGCTTTCGGGGATAAGATTTTGAATGGTGATTTTTCTATTGACGGTGAACAGAGTCGGTATGCGTCAGGAACAGATATTGTGAGGTTTCCACAAGGTGGTTATATGCATAGTTTCCCTTTCGTTGATAGCTCAGAGAGTGGGGCAGCTCAGAGCATACTAACATCTAGCATGAGTAAGGTAGTCATAAGCATCTCTATGAAATTTCATCTTAAAATAATTTTTGTGGGACCAAAAATCTTTTCAGATTTGACTAAATAACCAGTTTCCTATTTTGAAAATTAAGTAATGAGGAACAACTCTGTATTTTAGTTGAATAGATCCTTAAAACAGTTAATATCTTGCACAAGAGAGAATTAATAAGTTGAGCAGCAGCATATCTATTTAATGTGTGATAAGTTAGTTTCTTGGTTCCTGCGAAAGGAGCATGACACCACAGAAGACTTGTAAAGAACATTTAAATAGTTAAATATCTCGAGACTGTCAGAAAGAATGTTTTCATGCTTGGATTATAGATTTGTTATGTTTTCTTCTTGACATCGAGATTCTTCCATATAAAGGTCTTGCATTCATCCTTTCATATTGTGATCTTTGTAATTCCTTTAAGCTAACTGTTCATCAACAAATGCAGGCTGACTTTCCGATTCTTGGCCTATTAGAGGCAGGAGAAGGCCGCATTGCAGTCTATGGAGATTCCAATTGTCTAGATAGCAGCCATATGGTTACAAATTGCTATTGGCTGCTGAGAAAAATATTAGATTTTACAAGTGCAAATATTAGAGATCCGATACTATTCACCAAGTTTTCCAAACGAAATTCACCTCTGTATCTAGAAGACAGCAAACTACCATCTCGAAGAAGTGATGTAAATTTCTCTTTGTATTCTGCCGTTGCTCGAAAGGAATTGATATGCAGAAGTGATTCTAGGTTTGAAGTATGGGGGACCAAGGGATACAGTATACAAGTCCGGGGAAGAAATCGAAGACTTCCTGGATTTCCTGTAATTGACTTGGGACGAGGCTTGAATTCTACTTCAGAAGGTTCTTCAATGGGACCTCCGAAGTCGACTTCAAAAGACCGAAGTGATACATATGGAAACCGATATTTAAGCTTGTTCTACCGGGATGAGGTGAGGTTAAACTTTCCCGTCGATTTGAGACAAATTACTAATATATATTATTTCATCAATATGATTCTTAATTGTTCTTCCTCGCAGCCTGACATGCCGTTGATTGTTCCTAATCATTGGCTTGTTCCTGCTGTAGTTGCATTAACAGGTCATTATATATTCCTCGTTGCTCCTGAATTGGTTTCCTTTTCCTAAATGTATACCTCACTCGGTTTCTACTCTTTTAGGGCTTTTTCTCCTTTTGAGCTTTTGGCGGATTAGACAGAAGCGACGACGGCGACGAAGAGGATCTGGATCTGCACGTTTTTCCAATATATAG

mRNA sequence

ATGGATGTATTAAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCATTTGTAGAGAAGATTTGGGAAATAACAGCAAATAATATCATCATGGTTTCTGCAATTGGAAATGATGGACCACTTTATGGGACTTTAAACAATCCTGCAGATCAGAGTGATGTCATTGGTGTTGGGGGTATTGACTACAATGATCATATTGCTTCATTTTCCTCACGTGGCATGACTACTTGGGAGATGCCTCATGGTTATGGTCGTGTGAAGCCCGATGTTGTTGCATATGGTCGGGAAATTATGGGATCCAAAATTAGTACTGGATGCAAAAGTTTATCAGGCACTAGTGTCGCTAGTCCTGTTGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCTGAAAGCCAGCGGAAGGTTATTTTAAATCCAGCCAGCATGAAACAAGCACTTGTTGAGGGGGCTGCAAAGCTTGCTGGTCCTAATATGTATGAGCAAGGAGCTGGCAGAGTTGATCTGTTAGAATCGTATGAAGTACTTAAGAGCTACCAACCCAGGGCAAGTATCTTCCCTGGTGTTCTTGACTATACAGATTGCCCTTATACATGGCCCTTTTGCCGCCAACCTCTTTATGCAGGTGCGATGCCTATTATTTTCAATGCTACCATTTTGAATGGTATGGGTGTAATTGGCTATGTTGAAGGTCAACCAACTTGGCACCCTTCTGATGAAGAAGGCAATCTCTTAAGTATTCACTTCACTTATTCTAAGGTCATTTGGCCATGGACTGGTTATATTGCACTTCATATGCAAATTAAGGAAGAAGGTGCACAATTTTCTGGAGAAATTGAGGGCAATGTGACTCTAACAGTATATAGTCCTCCATCAAGAGGCGAGAAGAACCGCAGGATTAGTACTTGTGTCCTACAGCTGAAGCTAAAAGTAGTTCCTACACCTCCAAGATCAAAACGAATTTTATGGGATCAATTTCATAATATCAAATATCCTCCTGGTTATATTCCTAGAGATTCCTTGGATGTACGCAATGACATTCTGGACTGGCATGGAGACCACCTGCACACAAATTTTCATATCATGTTTAACATGTTAAGAGATGCTGGGTACTATGTTGAAACCCTTGGTTCTCCTCTTACATGCTTTGATGCTCGTCAATATGGAACACTTCTACTAGTGGATCTTGAAGACGAATACTTCAAAGAGGAAATTGAGAAGTTGAGGGACGATGTTATGACTACTGGATTGGGATTGGCTGTGTTTTCCGAATGGTATAATGTAGAAACAATGGTAAAGATGAGATTCTTTGATGATAACACAAGAAGTTGGTGGACACCAGTTACTGGAGGTGCAAATATTCCTGCACTCAATGATCTCTTAGCCCCATTTGGCATTGCTTTCGGGGATAAGATTTTGAATGGTGATTTTTCTATTGACGGTGAACAGAGTCGGTATGCGTCAGGAACAGATATTGTGAGGTTTCCACAAGGTGGTTATATGCATAGTTTCCCTTTCGTTGATAGCTCAGAGAGTGGGGCAGCTCAGAGCATACTAACATCTAGCATGAGTAAGGCTGACTTTCCGATTCTTGGCCTATTAGAGGCAGGAGAAGGCCGCATTGCAGTCTATGGAGATTCCAATTGTCTAGATAGCAGCCATATGGTTACAAATTGCTATTGGCTGCTGAGAAAAATATTAGATTTTACAAGTGCAAATATTAGAGATCCGATACTATTCACCAAGTTTTCCAAACGAAATTCACCTCTGTATCTAGAAGACAGCAAACTACCATCTCGAAGAAGTGATGTAAATTTCTCTTTGTATTCTGCCGTTGCTCGAAAGGAATTGATATGCAGAAGTGATTCTAGGTTTGAAGTATGGGGGACCAAGGGATACAGTATACAAGTCCGGGGAAGAAATCGAAGACTTCCTGGATTTCCTGTAATTGACTTGGGACGAGGCTTGAATTCTACTTCAGAAGGTTCTTCAATGGGACCTCCGAAGTCGACTTCAAAAGACCGAAGTGATACATATGGAAACCGATATTTAAGCTTGTTCTACCGGGATGAGCCTGACATGCCGTTGATTGTTCCTAATCATTGGCTTGTTCCTGCTGTAGTTGCATTAACAGGGCTTTTTCTCCTTTTGAGCTTTTGGCGGATTAGACAGAAGCGACGACGGCGACGAAGAGGATCTGGATCTGCACGTTTTTCCAATATATAG

Coding sequence (CDS)

ATGGATGTATTAAATTTAAGCATTGGTGGACCAGATTACTTGGATCTTCCATTTGTAGAGAAGATTTGGGAAATAACAGCAAATAATATCATCATGGTTTCTGCAATTGGAAATGATGGACCACTTTATGGGACTTTAAACAATCCTGCAGATCAGAGTGATGTCATTGGTGTTGGGGGTATTGACTACAATGATCATATTGCTTCATTTTCCTCACGTGGCATGACTACTTGGGAGATGCCTCATGGTTATGGTCGTGTGAAGCCCGATGTTGTTGCATATGGTCGGGAAATTATGGGATCCAAAATTAGTACTGGATGCAAAAGTTTATCAGGCACTAGTGTCGCTAGTCCTGTTGTTGCTGGTGTGGTATGTCTACTTGTTAGTGTCATCCCTGAAAGCCAGCGGAAGGTTATTTTAAATCCAGCCAGCATGAAACAAGCACTTGTTGAGGGGGCTGCAAAGCTTGCTGGTCCTAATATGTATGAGCAAGGAGCTGGCAGAGTTGATCTGTTAGAATCGTATGAAGTACTTAAGAGCTACCAACCCAGGGCAAGTATCTTCCCTGGTGTTCTTGACTATACAGATTGCCCTTATACATGGCCCTTTTGCCGCCAACCTCTTTATGCAGGTGCGATGCCTATTATTTTCAATGCTACCATTTTGAATGGTATGGGTGTAATTGGCTATGTTGAAGGTCAACCAACTTGGCACCCTTCTGATGAAGAAGGCAATCTCTTAAGTATTCACTTCACTTATTCTAAGGTCATTTGGCCATGGACTGGTTATATTGCACTTCATATGCAAATTAAGGAAGAAGGTGCACAATTTTCTGGAGAAATTGAGGGCAATGTGACTCTAACAGTATATAGTCCTCCATCAAGAGGCGAGAAGAACCGCAGGATTAGTACTTGTGTCCTACAGCTGAAGCTAAAAGTAGTTCCTACACCTCCAAGATCAAAACGAATTTTATGGGATCAATTTCATAATATCAAATATCCTCCTGGTTATATTCCTAGAGATTCCTTGGATGTACGCAATGACATTCTGGACTGGCATGGAGACCACCTGCACACAAATTTTCATATCATGTTTAACATGTTAAGAGATGCTGGGTACTATGTTGAAACCCTTGGTTCTCCTCTTACATGCTTTGATGCTCGTCAATATGGAACACTTCTACTAGTGGATCTTGAAGACGAATACTTCAAAGAGGAAATTGAGAAGTTGAGGGACGATGTTATGACTACTGGATTGGGATTGGCTGTGTTTTCCGAATGGTATAATGTAGAAACAATGGTAAAGATGAGATTCTTTGATGATAACACAAGAAGTTGGTGGACACCAGTTACTGGAGGTGCAAATATTCCTGCACTCAATGATCTCTTAGCCCCATTTGGCATTGCTTTCGGGGATAAGATTTTGAATGGTGATTTTTCTATTGACGGTGAACAGAGTCGGTATGCGTCAGGAACAGATATTGTGAGGTTTCCACAAGGTGGTTATATGCATAGTTTCCCTTTCGTTGATAGCTCAGAGAGTGGGGCAGCTCAGAGCATACTAACATCTAGCATGAGTAAGGCTGACTTTCCGATTCTTGGCCTATTAGAGGCAGGAGAAGGCCGCATTGCAGTCTATGGAGATTCCAATTGTCTAGATAGCAGCCATATGGTTACAAATTGCTATTGGCTGCTGAGAAAAATATTAGATTTTACAAGTGCAAATATTAGAGATCCGATACTATTCACCAAGTTTTCCAAACGAAATTCACCTCTGTATCTAGAAGACAGCAAACTACCATCTCGAAGAAGTGATGTAAATTTCTCTTTGTATTCTGCCGTTGCTCGAAAGGAATTGATATGCAGAAGTGATTCTAGGTTTGAAGTATGGGGGACCAAGGGATACAGTATACAAGTCCGGGGAAGAAATCGAAGACTTCCTGGATTTCCTGTAATTGACTTGGGACGAGGCTTGAATTCTACTTCAGAAGGTTCTTCAATGGGACCTCCGAAGTCGACTTCAAAAGACCGAAGTGATACATATGGAAACCGATATTTAAGCTTGTTCTACCGGGATGAGCCTGACATGCCGTTGATTGTTCCTAATCATTGGCTTGTTCCTGCTGTAGTTGCATTAACAGGGCTTTTTCTCCTTTTGAGCTTTTGGCGGATTAGACAGAAGCGACGACGGCGACGAAGAGGATCTGGATCTGCACGTTTTTCCAATATATAG

Protein sequence

MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGGIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKSYQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPSDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNRRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLPSRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNSTSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLSFWRIRQKRRRRRRGSGSARFSNI*
Homology
BLAST of CsGy1G016630 vs. ExPASy Swiss-Prot
Match: Q0WUG6 (Subtilisin-like protease SBT6.1 OS=Arabidopsis thaliana OX=3702 GN=SBT6.1 PE=1 SV=1)

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 595/747 (79.65%), Postives = 663/747 (88.76%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEK+WEITA+NIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 296  MDVLNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 355

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            ID +DHIASFSSRGM+TWE+PHGYGRVKPDVVAYGR+IMGSKISTGCKSLSGTSVASPVV
Sbjct: 356  IDNDDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSLSGTSVASPVV 415

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AG+VCLLVSVIPE++RK +LNPASMKQALVEGAAKL+GPNMYEQGAGRVDLLESYE+LKS
Sbjct: 416  AGIVCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVDLLESYEILKS 475

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            Y PRASIFP +LDY DCPY+WPFCRQPLYAGAMPIIFN TILNGMGVIGY+E  PTWHP+
Sbjct: 476  YHPRASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGYIESPPTWHPA 535

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            +EEGNLLSIHF Y  VIWPWTGY+ALHMQIKEEGAQF+GEIEGNVT+ VYSPP+ GE   
Sbjct: 536  NEEGNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVYSPPASGESGP 595

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            R STC LQLKLKV+PTPPR+KRILWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 596  RRSTCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 655

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIM+NMLRDAGYY+ETLGSPLTCFDA+QYGTLL+VDLED+YF EEIEKLRDDV+ TGLG
Sbjct: 656  FHIMYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKLRDDVINTGLG 715

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            L VF+EWYNV+TMVKMRFFDDNTRSWWTPVTGGANIPALN+LLA FGIAFGDKILNGDFS
Sbjct: 716  LVVFAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAFGDKILNGDFS 775

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGT+IVRFP GG++H+FP +DSSESGA Q++L +  SK D  +LGLLE GE
Sbjct: 776  IDGEQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDPAVLGLLEIGE 835

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GR+ VYGDSNCLDSSHMVTNCYWLL+K+LDF+S+NI+DP+LF+KF+KR SP+ +++ +LP
Sbjct: 836  GRVGVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRYSPVIIDEKQLP 895

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRR+DVNFS YS+V  KELIC SDSRFEVWGTKGY++ VRGRNRRLPG+  IDLGRGLN 
Sbjct: 896  SRRTDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGYHGIDLGRGLNF 955

Query: 661  TSEGS------SMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTG 720
            T E        S       S  RS + G     LF RDE DMP +VP  W+V A V  +G
Sbjct: 956  TVESKRPTRWRSAKEGGELSSSRSKSLG----GLFNRDEIDMPFLVPTRWIVLAGVVASG 1015

Query: 721  LFLLLSFWRIRQKRRRRRRGSGSARFS 742
            + +LLS WRIRQKR RRRR SGS R +
Sbjct: 1016 VLVLLSIWRIRQKRGRRRRASGSNRLA 1038

BLAST of CsGy1G016630 vs. ExPASy Swiss-Prot
Match: Q14703 (Membrane-bound transcription factor site-1 protease OS=Homo sapiens OX=9606 GN=MBTPS1 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 5.1e-203
Identity = 333/625 (53.28%), Postives = 439/625 (70.24%), Query Frame = 0

Query: 1   MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
           +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTLNNPADQ DVIGVGG
Sbjct: 301 IDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGG 360

Query: 61  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
           ID+ D+IA FSSRGMTTWE+P GYGR+KPD+V YG  + GS +  GC++LSGTSVASPVV
Sbjct: 361 IDFEDNIARFSSRGMTTWELPGGYGRMKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVV 420

Query: 121 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
           AG V LLVS +   Q++ ++NPASMKQAL+  A +L G NM+EQG G++DLL +Y++L S
Sbjct: 421 AGAVTLLVSTV---QKRELVNPASMKQALIASARRLPGVNMFEQGHGKLDLLRAYQILNS 480

Query: 181 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHP- 240
           Y+P+AS+ P  +D T+CPY WP+C QP+Y G MP + N TILNGMGV G +  +P W P 
Sbjct: 481 YKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTVVNVTILNGMGVTGRIVDKPDWQPY 540

Query: 241 SDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKN 300
             + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G+V +TV SP     KN
Sbjct: 541 LPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHVMITVASPAETESKN 600

Query: 301 --RRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 360
              + ST  L +K+K++PTPPRSKR+LWDQ+HN++YPPGY PRD+L ++ND LDW+GDH+
Sbjct: 601 GAEQTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHI 660

Query: 361 HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTT 420
           HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLL+VD E+EYF EEI KLR DV   
Sbjct: 661 HTNFRDMYQHLRSMGYFVEVLGAPFTCFDASQYGTLLMVDSEEEYFPEEIAKLRRDV-DN 720

Query: 421 GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 480
           GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+LL+ + + F D +  G
Sbjct: 721 GLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELLSVWNMGFSDGLYEG 780

Query: 481 DFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLE 540
           +F++      YASG  I +FP+ G + +  F D       + +   +    + PILGL +
Sbjct: 781 EFTLANHDMYYASGCSIAKFPEDGVVITQTFKDQ----GLEVLKQETAVVENVPILGLYQ 840

Query: 541 ---AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYL 600
               G GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P L +    R  P   
Sbjct: 841 IPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSL-SHSGNRQRPPSG 900

Query: 601 EDSKLPSRRSDVNFSLYSAVARKEL 620
             S  P R    +   YS V    L
Sbjct: 901 AGSVTPERMEGNHLHRYSKVLEAHL 916

BLAST of CsGy1G016630 vs. ExPASy Swiss-Prot
Match: Q9WTZ3 (Membrane-bound transcription factor site-1 protease OS=Rattus norvegicus OX=10116 GN=Mbtps1 PE=1 SV=2)

HSP 1 Score: 706.1 bits (1821), Expect = 4.3e-202
Identity = 328/598 (54.85%), Postives = 431/598 (72.07%), Query Frame = 0

Query: 1   MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
           +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTLNNPADQ DVIGVGG
Sbjct: 301 IDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGG 360

Query: 61  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
           ID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  GC++LSGTSVASPVV
Sbjct: 361 IDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVV 420

Query: 121 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
           AG V LLVS +   Q++ ++NPAS+KQAL+  A +L G NM+EQG G++DLL +Y++L S
Sbjct: 421 AGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSS 480

Query: 181 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHP- 240
           Y+P+AS+ P  +D T+CPY WP+C QP+Y G MP I N TILNGMGV G +  +P W P 
Sbjct: 481 YKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPY 540

Query: 241 SDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKN 300
             + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G++ +TV SP     KN
Sbjct: 541 LPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELKN 600

Query: 301 --RRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 360
                ST  L +K+K++PTPPRSKR+LWDQ+HN++YPPGY PRD+L ++ND LDW+GDH+
Sbjct: 601 GAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHV 660

Query: 361 HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTT 420
           HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLL+VD E+EYF EEI KLR DV   
Sbjct: 661 HTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDV-DN 720

Query: 421 GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 480
           GL L VFS+WYN   M K++F+D+NTR WW P TGGAN+PALN+LL+ + + F D +  G
Sbjct: 721 GLSLVVFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNELLSVWNMGFSDGLYEG 780

Query: 481 DFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKAD-FPILGLL 540
           +F++      YASG  I RFP+ G + +  F D         +L    +  D  PILGL 
Sbjct: 781 EFALANHDMYYASGCSIARFPEDGVVITQTFKDQ-----GLEVLKQETAVVDNVPILGLY 840

Query: 541 E---AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSP 592
           +    G GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P L    +++  P
Sbjct: 841 QIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPP 889

BLAST of CsGy1G016630 vs. ExPASy Swiss-Prot
Match: Q9Z2A8 (Membrane-bound transcription factor site-1 protease OS=Cricetulus griseus OX=10029 GN=MBTPS1 PE=1 SV=2)

HSP 1 Score: 705.3 bits (1819), Expect = 7.4e-202
Identity = 325/597 (54.44%), Postives = 431/597 (72.19%), Query Frame = 0

Query: 1   MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
           +DVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTLNNPADQ DVIGVGG
Sbjct: 301 IDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGG 360

Query: 61  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
           ID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  GC++LSGTSVASPVV
Sbjct: 361 IDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVV 420

Query: 121 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
           AG V LLVS +   Q++ ++NPAS+KQAL+  A +L G NM+EQG G++DLL +Y++L S
Sbjct: 421 AGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSS 480

Query: 181 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHP- 240
           Y+P+AS+ P  +D T+CPY WP+C QP+Y G MP I N TILNGMGV G +  +P W P 
Sbjct: 481 YKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPY 540

Query: 241 SDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKN 300
             + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G++ +TV SP     KN
Sbjct: 541 LPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETEAKN 600

Query: 301 --RRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 360
                ST  L +K+K++PTPPRSKR+LWDQ+HN++YPPGY PRD+L ++ND LDW+GDH+
Sbjct: 601 GAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHV 660

Query: 361 HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTT 420
           HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLL+VD E+EYF EEI KLR DV   
Sbjct: 661 HTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLMVDSEEEYFPEEIAKLRRDV-DN 720

Query: 421 GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 480
           GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+LL+ + + F D +  G
Sbjct: 721 GLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELLSVWNMGFSDGLYEG 780

Query: 481 DFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLE 540
           +F++      YASG  I +FP+ G + +  F D       + +   +    + PILGL +
Sbjct: 781 EFALANHDMYYASGCSIAKFPEDGVVITQTFKDQ----GLEVLKQETAVVENVPILGLYQ 840

Query: 541 ---AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSP 592
               G GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P L    +++  P
Sbjct: 841 IPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPP 889

BLAST of CsGy1G016630 vs. ExPASy Swiss-Prot
Match: Q9WTZ2 (Membrane-bound transcription factor site-1 protease OS=Mus musculus OX=10090 GN=Mbtps1 PE=1 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.1e-200
Identity = 325/597 (54.44%), Postives = 429/597 (71.86%), Query Frame = 0

Query: 1   MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
           MDVLNLSIGGPD++D PFV+K+WE+TANN+IMVSAIGNDGPLYGTLNNPADQ DVIGVGG
Sbjct: 301 MDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGG 360

Query: 61  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
           ID+ D+IA FSSRGMTTWE+P GYGRVKPD+V YG  + GS +  GC++LSGTSVASPVV
Sbjct: 361 IDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVV 420

Query: 121 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
           AG V LLVS +   Q++ ++NPAS+KQAL+  A +L G NM+EQG G++DLL +Y++L S
Sbjct: 421 AGAVTLLVSTV---QKRELVNPASVKQALIASARRLPGVNMFEQGHGKLDLLRAYQILSS 480

Query: 181 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHP- 240
           Y+P+AS+ P  +D T+CPY WP+C QP+Y G MP I N TILNGMGV G +  +P W P 
Sbjct: 481 YKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPEWRPY 540

Query: 241 SDEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKN 300
             + G+ + + F+YS V+WPW+GY+A+ + + ++ A + G  +G++ +TV SP      +
Sbjct: 541 LPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHIMITVASPAETELHS 600

Query: 301 --RRISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHL 360
                ST  L +K+K++PTPPRSKR+LWDQ+HN++YPPGY PRD+L ++ND LDW+GDH+
Sbjct: 601 GAEHTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHV 660

Query: 361 HTNFHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTT 420
           HTNF  M+  LR  GY+VE LG+P TCFDA QYGTLLLVD E+EYF EEI KLR DV   
Sbjct: 661 HTNFRDMYQHLRSMGYFVEVLGAPFTCFDATQYGTLLLVDSEEEYFPEEIAKLRRDV-DN 720

Query: 421 GLGLAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNG 480
           GL L +FS+WYN   M K++F+D+NTR WW P TGGANIPALN+LL+ + + F D +  G
Sbjct: 721 GLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELLSVWNMGFSDGLYEG 780

Query: 481 DFSIDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLE 540
           +F +      YASG  I +FP+ G + +  F D       + +   +    + PILGL +
Sbjct: 781 EFVLANHDMYYASGCSIAKFPEDGVVITQTFKDQ----GLEVLKQETAVVENVPILGLYQ 840

Query: 541 ---AGEGRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSP 592
               G GRI +YGDSNCLD SH   +C+WLL  +L +TS  +  P L    +++  P
Sbjct: 841 IPSEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPP 889

BLAST of CsGy1G016630 vs. NCBI nr
Match: KGN65332.2 (hypothetical protein Csa_017774 [Cucumis sativus])

HSP 1 Score: 1520 bits (3936), Expect = 0.0
Identity = 742/743 (99.87%), Postives = 743/743 (100.00%), Query Frame = 0

Query: 1   MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
           MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 32  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 91

Query: 61  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
           IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 92  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 151

Query: 121 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
           AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS
Sbjct: 152 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 211

Query: 181 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
           YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS
Sbjct: 212 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 271

Query: 241 DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
           DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 272 DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 331

Query: 301 RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
           RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 332 RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 391

Query: 361 FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
           FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG
Sbjct: 392 FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 451

Query: 421 LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
           LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 452 LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 511

Query: 481 IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
           IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE
Sbjct: 512 IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 571

Query: 541 GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
           GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP
Sbjct: 572 GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 631

Query: 601 SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
           SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 632 SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 691

Query: 661 TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
           TSEGSSMGPPKS+SKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS
Sbjct: 692 TSEGSSMGPPKSSSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 751

Query: 721 FWRIRQKRRRRRRGSGSARFSNI 743
           FWRIRQKRRRRRRGSGSARFSNI
Sbjct: 752 FWRIRQKRRRRRRGSGSARFSNI 774

BLAST of CsGy1G016630 vs. NCBI nr
Match: XP_004150051.1 (subtilisin-like protease SBT6.1 [Cucumis sativus])

HSP 1 Score: 1520 bits (3936), Expect = 0.0
Identity = 742/743 (99.87%), Postives = 743/743 (100.00%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 303  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 362

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 363  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 422

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS
Sbjct: 423  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 482

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS
Sbjct: 483  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 542

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 543  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 602

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 603  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 662

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG
Sbjct: 663  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 722

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 723  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 782

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE
Sbjct: 783  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 842

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP
Sbjct: 843  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 902

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 903  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 962

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            TSEGSSMGPPKS+SKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS
Sbjct: 963  TSEGSSMGPPKSSSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 1022

Query: 721  FWRIRQKRRRRRRGSGSARFSNI 743
            FWRIRQKRRRRRRGSGSARFSNI
Sbjct: 1023 FWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of CsGy1G016630 vs. NCBI nr
Match: XP_008463395.1 (PREDICTED: subtilisin-like protease SBT6.1 [Cucumis melo])

HSP 1 Score: 1491 bits (3861), Expect = 0.0
Identity = 730/743 (98.25%), Postives = 733/743 (98.65%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 304  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 363

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 364  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 423

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS
Sbjct: 424  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 483

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS
Sbjct: 484  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 543

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEE NLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 544  DEEANLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 603

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 604  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 663

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG
Sbjct: 664  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 723

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 724  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 783

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFPQGGY+HSFPFVDSSESGAAQSILTSSMSKADF ILGLLEA E
Sbjct: 784  IDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFSILGLLEAAE 843

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSKR +PLYLEDSKLP
Sbjct: 844  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRKTPLYLEDSKLP 903

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAVA KELICRSDSRFEVWGTKGYS QVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 904  SRRSDVNFSLYSAVAGKELICRSDSRFEVWGTKGYSTQVRGRNRRLPGFPVIDLGRGLNS 963

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            TSE SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPL VPNHWLVPAVVALTGL LLLS
Sbjct: 964  TSE-SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLSVPNHWLVPAVVALTGLLLLLS 1023

Query: 721  FWRIRQKRRRRRRGSGSARFSNI 743
            FWRIRQKRRRRRRGSGSARFSNI
Sbjct: 1024 FWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of CsGy1G016630 vs. NCBI nr
Match: XP_038879333.1 (subtilisin-like protease SBT6.1 [Benincasa hispida])

HSP 1 Score: 1479 bits (3830), Expect = 0.0
Identity = 721/743 (97.04%), Postives = 729/743 (98.12%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 305  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 364

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 365  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 424

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYE+LKS
Sbjct: 425  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEILKS 484

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFP VLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHP 
Sbjct: 485  YQPRASIFPDVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPL 544

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 545  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 604

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 605  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 664

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNML DAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM TGLG
Sbjct: 665  FHIMFNMLSDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLG 724

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 725  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 784

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSR+ASGTDIVRFPQGGY+HSFPFVDSSESGAAQSILTSSMSKADFPILGLLEA E
Sbjct: 785  IDGEQSRFASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAIE 844

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GR+AVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSKR+SPLYLED+KLP
Sbjct: 845  GRVAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRDSPLYLEDNKLP 904

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAVA KEL+CRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 905  SRRSDVNFSLYSAVAGKELLCRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 964

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            T E SSMGPPK T KDR DTYGNRYLSLFYRDEPDMPLIVPN WLVPAVVALTGL LLLS
Sbjct: 965  TLESSSMGPPKLTLKDRRDTYGNRYLSLFYRDEPDMPLIVPNQWLVPAVVALTGLLLLLS 1024

Query: 721  FWRIRQKRRRRRRGSGSARFSNI 743
            FWRIRQKRRRRRRGSGSARFSNI
Sbjct: 1025 FWRIRQKRRRRRRGSGSARFSNI 1047

BLAST of CsGy1G016630 vs. NCBI nr
Match: KAG6589917.1 (Subtilisin-like protease 6.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023586.1 Subtilisin-like protease SBT6.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1462 bits (3786), Expect = 0.0
Identity = 712/744 (95.70%), Postives = 726/744 (97.58%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 310  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 369

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 370  IDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 429

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYE+LKS
Sbjct: 430  AGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEILKS 489

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW+PS
Sbjct: 490  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWNPS 549

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 550  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 609

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 610  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 669

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM TGLG
Sbjct: 670  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLG 729

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 730  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 789

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFP+GGY+HSFPF+DSSESGAAQSILTSS+SKADFPILGLLEA E
Sbjct: 790  IDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLEAAE 849

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSK+NSPLYLED+KLP
Sbjct: 850  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDNKLP 909

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAV+ KELIC SDSRFEVWGTKGYS+Q RGRNRRLPGFP+IDLGR LNS
Sbjct: 910  SRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRDLNS 969

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            T E S MGPPK T KDRSD YGNRYLSLFYRDEPDMPLIVPNHWLVPAVVA+ GL LLLS
Sbjct: 970  TPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLLLLS 1029

Query: 721  FWRIRQKRRRRRRGSGSA-RFSNI 743
            FWRIRQKRRRRRRGSGSA RFSN+
Sbjct: 1030 FWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of CsGy1G016630 vs. ExPASy TrEMBL
Match: A0A0A0LTP5 (Peptidase_S8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G250160 PE=3 SV=1)

HSP 1 Score: 1520 bits (3936), Expect = 0.0
Identity = 742/743 (99.87%), Postives = 743/743 (100.00%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 303  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 362

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 363  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 422

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS
Sbjct: 423  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 482

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS
Sbjct: 483  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 542

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 543  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 602

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 603  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 662

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG
Sbjct: 663  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 722

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 723  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 782

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE
Sbjct: 783  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 842

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP
Sbjct: 843  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 902

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 903  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 962

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            TSEGSSMGPPKS+SKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS
Sbjct: 963  TSEGSSMGPPKSSSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 1022

Query: 721  FWRIRQKRRRRRRGSGSARFSNI 743
            FWRIRQKRRRRRRGSGSARFSNI
Sbjct: 1023 FWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of CsGy1G016630 vs. ExPASy TrEMBL
Match: A0A1S3CJL6 (subtilisin-like protease SBT6.1 OS=Cucumis melo OX=3656 GN=LOC103501563 PE=3 SV=1)

HSP 1 Score: 1491 bits (3861), Expect = 0.0
Identity = 730/743 (98.25%), Postives = 733/743 (98.65%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 304  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 363

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 364  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 423

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS
Sbjct: 424  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 483

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS
Sbjct: 484  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 543

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEE NLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 544  DEEANLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 603

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 604  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 663

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG
Sbjct: 664  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 723

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 724  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 783

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFPQGGY+HSFPFVDSSESGAAQSILTSSMSKADF ILGLLEA E
Sbjct: 784  IDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFSILGLLEAAE 843

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSKR +PLYLEDSKLP
Sbjct: 844  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRKTPLYLEDSKLP 903

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAVA KELICRSDSRFEVWGTKGYS QVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 904  SRRSDVNFSLYSAVAGKELICRSDSRFEVWGTKGYSTQVRGRNRRLPGFPVIDLGRGLNS 963

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            TSE SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPL VPNHWLVPAVVALTGL LLLS
Sbjct: 964  TSE-SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLSVPNHWLVPAVVALTGLLLLLS 1023

Query: 721  FWRIRQKRRRRRRGSGSARFSNI 743
            FWRIRQKRRRRRRGSGSARFSNI
Sbjct: 1024 FWRIRQKRRRRRRGSGSARFSNI 1045

BLAST of CsGy1G016630 vs. ExPASy TrEMBL
Match: A0A6J1H8D8 (subtilisin-like protease SBT6.1 OS=Cucurbita moschata OX=3662 GN=LOC111461434 PE=3 SV=1)

HSP 1 Score: 1461 bits (3782), Expect = 0.0
Identity = 711/744 (95.56%), Postives = 725/744 (97.45%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 310  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 369

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 370  IDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 429

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYE+LKS
Sbjct: 430  AGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEILKS 489

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW+PS
Sbjct: 490  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWNPS 549

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 550  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 609

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 610  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 669

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM TGLG
Sbjct: 670  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLG 729

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 730  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 789

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFP+GGY+HSFPF+DSSESGAAQSILTSS+SKADFPILGLLEA E
Sbjct: 790  IDGEQSRYASGTDIVRFPRGGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLEAAE 849

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSK+NSPLYLED+KLP
Sbjct: 850  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDNKLP 909

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSD NFSLYSAV+ KELIC SDSRFEVWGTKGYS+Q RGRNRRLPGFP+IDLGR LNS
Sbjct: 910  SRRSDANFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRDLNS 969

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            T E S MGPPK T KDRSD YGNRYLSLFYRDEPDMPLIVPNHWLVPAVVA+ GL LLLS
Sbjct: 970  TPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLLLLS 1029

Query: 721  FWRIRQKRRRRRRGSGSA-RFSNI 743
            FWRIRQKRRRRRRGSGSA RFSN+
Sbjct: 1030 FWRIRQKRRRRRRGSGSAARFSNL 1053

BLAST of CsGy1G016630 vs. ExPASy TrEMBL
Match: A0A6J1JAS5 (subtilisin-like protease SBT6.1 OS=Cucurbita maxima OX=3661 GN=LOC111485106 PE=3 SV=1)

HSP 1 Score: 1459 bits (3777), Expect = 0.0
Identity = 711/744 (95.56%), Postives = 725/744 (97.45%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 310  MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 369

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            IDYNDHIASFSSRGMTTWEMPHG GRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 370  IDYNDHIASFSSRGMTTWEMPHGNGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 429

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AGVVCLLVSVIPESQRK ILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYE+LKS
Sbjct: 430  AGVVCLLVSVIPESQRKTILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEILKS 489

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTW+PS
Sbjct: 490  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWNPS 549

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 550  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 609

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            RISTCVLQLKLKVV TPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 610  RISTCVLQLKLKVVSTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 669

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVM TGLG
Sbjct: 670  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMITGLG 729

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 730  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 789

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGTDIVRFP+ GY+HSFPF+DSSESGAAQSILTSS+SKADFPILGLLEAGE
Sbjct: 790  IDGEQSRYASGTDIVRFPRDGYVHSFPFIDSSESGAAQSILTSSLSKADFPILGLLEAGE 849

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSK+NSPLYLED+KLP
Sbjct: 850  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKQNSPLYLEDNKLP 909

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRRSDVNFSLYSAV+ KELIC SDSRFEVWGTKGYS+Q RGRNRRLPGFP+IDLGR LNS
Sbjct: 910  SRRSDVNFSLYSAVSGKELICMSDSRFEVWGTKGYSVQGRGRNRRLPGFPLIDLGRDLNS 969

Query: 661  TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTGLFLLLS 720
            T E S MGPPK T KDRSD YGNRYLSLFYRDEPDMPLIVPNHWLVPAVVA+ GL LLLS
Sbjct: 970  TPESSLMGPPKLTLKDRSDKYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVAVAGLLLLLS 1029

Query: 721  FWRIRQKRRRRRRGSGSA-RFSNI 743
            FWRIRQKRRRRRRGSGSA RFSN+
Sbjct: 1030 FWRIRQKRRRRRRGSGSAPRFSNL 1053

BLAST of CsGy1G016630 vs. ExPASy TrEMBL
Match: A0A5A7TUN6 (Subtilisin-like protease SBT6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00820 PE=3 SV=1)

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 700/713 (98.18%), Postives = 704/713 (98.74%), Query Frame = 0

Query: 1   MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
           MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 243 MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 302

Query: 61  IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
           IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV
Sbjct: 303 IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 362

Query: 121 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
           AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS
Sbjct: 363 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 422

Query: 181 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
           YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS
Sbjct: 423 YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 482

Query: 241 DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
           DEE NLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR
Sbjct: 483 DEEANLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 542

Query: 301 RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
           RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 543 RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 602

Query: 361 FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
           FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG
Sbjct: 603 FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 662

Query: 421 LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
           LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS
Sbjct: 663 LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 722

Query: 481 IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
           IDGEQSRYASGTDIVRFPQGGY+HSFPFVDSSESGAAQSILTSSMSKADF ILGLLEA E
Sbjct: 723 IDGEQSRYASGTDIVRFPQGGYVHSFPFVDSSESGAAQSILTSSMSKADFSILGLLEAAE 782

Query: 541 GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
           GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTS NIRDP+LFTKFSKR +PLYLEDSKLP
Sbjct: 783 GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSGNIRDPVLFTKFSKRKAPLYLEDSKLP 842

Query: 601 SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
           SRRSD+NFSLYSAVA KELICRSDSRFEVWGTKGYS QVRGRNRRLPGFPVIDLGRGLNS
Sbjct: 843 SRRSDINFSLYSAVAGKELICRSDSRFEVWGTKGYSTQVRGRNRRLPGFPVIDLGRGLNS 902

Query: 661 TSEGSSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALT 713
           TSE SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPL VPNHWLVPAVVALT
Sbjct: 903 TSE-SSMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLSVPNHWLVPAVVALT 954

BLAST of CsGy1G016630 vs. TAIR 10
Match: AT5G19660.1 (SITE-1 protease )

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 595/747 (79.65%), Postives = 663/747 (88.76%), Query Frame = 0

Query: 1    MDVLNLSIGGPDYLDLPFVEKIWEITANNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 60
            MDVLNLSIGGPDYLDLPFVEK+WEITA+NIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG
Sbjct: 296  MDVLNLSIGGPDYLDLPFVEKVWEITASNIIMVSAIGNDGPLYGTLNNPADQSDVIGVGG 355

Query: 61   IDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKISTGCKSLSGTSVASPVV 120
            ID +DHIASFSSRGM+TWE+PHGYGRVKPDVVAYGR+IMGSKISTGCKSLSGTSVASPVV
Sbjct: 356  IDNDDHIASFSSRGMSTWELPHGYGRVKPDVVAYGRDIMGSKISTGCKSLSGTSVASPVV 415

Query: 121  AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKLAGPNMYEQGAGRVDLLESYEVLKS 180
            AG+VCLLVSVIPE++RK +LNPASMKQALVEGAAKL+GPNMYEQGAGRVDLLESYE+LKS
Sbjct: 416  AGIVCLLVSVIPEARRKDLLNPASMKQALVEGAAKLSGPNMYEQGAGRVDLLESYEILKS 475

Query: 181  YQPRASIFPGVLDYTDCPYTWPFCRQPLYAGAMPIIFNATILNGMGVIGYVEGQPTWHPS 240
            Y PRASIFP +LDY DCPY+WPFCRQPLYAGAMPIIFN TILNGMGVIGY+E  PTWHP+
Sbjct: 476  YHPRASIFPSILDYNDCPYSWPFCRQPLYAGAMPIIFNTTILNGMGVIGYIESPPTWHPA 535

Query: 241  DEEGNLLSIHFTYSKVIWPWTGYIALHMQIKEEGAQFSGEIEGNVTLTVYSPPSRGEKNR 300
            +EEGNLLSIHF Y  VIWPWTGY+ALHMQIKEEGAQF+GEIEGNVT+ VYSPP+ GE   
Sbjct: 536  NEEGNLLSIHFKYPDVIWPWTGYLALHMQIKEEGAQFTGEIEGNVTVKVYSPPASGESGP 595

Query: 301  RISTCVLQLKLKVVPTPPRSKRILWDQFHNIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 360
            R STC LQLKLKV+PTPPR+KRILWDQFH+IKYPPGYIPRDSLDVRNDILDWHGDHLHTN
Sbjct: 596  RRSTCSLQLKLKVIPTPPRAKRILWDQFHSIKYPPGYIPRDSLDVRNDILDWHGDHLHTN 655

Query: 361  FHIMFNMLRDAGYYVETLGSPLTCFDARQYGTLLLVDLEDEYFKEEIEKLRDDVMTTGLG 420
            FHIM+NMLRDAGYY+ETLGSPLTCFDA+QYGTLL+VDLED+YF EEIEKLRDDV+ TGLG
Sbjct: 656  FHIMYNMLRDAGYYIETLGSPLTCFDAQQYGTLLMVDLEDDYFPEEIEKLRDDVINTGLG 715

Query: 421  LAVFSEWYNVETMVKMRFFDDNTRSWWTPVTGGANIPALNDLLAPFGIAFGDKILNGDFS 480
            L VF+EWYNV+TMVKMRFFDDNTRSWWTPVTGGANIPALN+LLA FGIAFGDKILNGDFS
Sbjct: 716  LVVFAEWYNVDTMVKMRFFDDNTRSWWTPVTGGANIPALNNLLASFGIAFGDKILNGDFS 775

Query: 481  IDGEQSRYASGTDIVRFPQGGYMHSFPFVDSSESGAAQSILTSSMSKADFPILGLLEAGE 540
            IDGEQSRYASGT+IVRFP GG++H+FP +DSSESGA Q++L +  SK D  +LGLLE GE
Sbjct: 776  IDGEQSRYASGTNIVRFPAGGFLHTFPLLDSSESGATQNLLLTEASKEDPAVLGLLEIGE 835

Query: 541  GRIAVYGDSNCLDSSHMVTNCYWLLRKILDFTSANIRDPILFTKFSKRNSPLYLEDSKLP 600
            GR+ VYGDSNCLDSSHMVTNCYWLL+K+LDF+S+NI+DP+LF+KF+KR SP+ +++ +LP
Sbjct: 836  GRVGVYGDSNCLDSSHMVTNCYWLLKKMLDFSSSNIKDPVLFSKFAKRYSPVIIDEKQLP 895

Query: 601  SRRSDVNFSLYSAVARKELICRSDSRFEVWGTKGYSIQVRGRNRRLPGFPVIDLGRGLNS 660
            SRR+DVNFS YS+V  KELIC SDSRFEVWGTKGY++ VRGRNRRLPG+  IDLGRGLN 
Sbjct: 896  SRRTDVNFSTYSSVIGKELICESDSRFEVWGTKGYNLHVRGRNRRLPGYHGIDLGRGLNF 955

Query: 661  TSEGS------SMGPPKSTSKDRSDTYGNRYLSLFYRDEPDMPLIVPNHWLVPAVVALTG 720
            T E        S       S  RS + G     LF RDE DMP +VP  W+V A V  +G
Sbjct: 956  TVESKRPTRWRSAKEGGELSSSRSKSLG----GLFNRDEIDMPFLVPTRWIVLAGVVASG 1015

Query: 721  LFLLLSFWRIRQKRRRRRRGSGSARFS 742
            + +LLS WRIRQKR RRRR SGS R +
Sbjct: 1016 VLVLLSIWRIRQKRGRRRRASGSNRLA 1038

BLAST of CsGy1G016630 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 47.4 bits (111), Expect = 5.9e-05
Identity = 47/155 (30.32%), Postives = 78/155 (50.32%), Query Frame = 0

Query: 60  GIDYNDHIASFSSRGMTTWEMPHGYGRVKPDVVAYGREIM-----GSKIST-GCKSLSGT 119
           G   +  +A+FSSRG  +         +KPD+ A G  I+      S I+  G   +SGT
Sbjct: 478 GQSVSTKVATFSSRGPNSVSP----AILKPDIAAPGVNILAAISPNSSINDGGFAMMSGT 537

Query: 120 SVASPVVAGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKL--AGPNMYEQGAGRVDL 179
           S+A+PVV+GVV LL S+ P+       +P+++K A+V  A +   +G  ++  G+ R  L
Sbjct: 538 SMATPVVSGVVVLLKSLHPD------WSPSAIKSAIVTTAWRTDPSGEPIFADGSSR-KL 597

Query: 180 LESYEVLKS-YQPRASIFPGVL-DYTDCPYTWPFC 205
            + ++       P  ++ PG++ D T   Y    C
Sbjct: 598 ADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMC 621

BLAST of CsGy1G016630 vs. TAIR 10
Match: AT4G21640.1 (Subtilase family protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.9e-04
Identity = 36/110 (32.73%), Postives = 60/110 (54.55%), Query Frame = 0

Query: 67  IASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGS------KISTGCKSLSGTSVASPVV 126
           +A+FSSRG  +         +KPD+ A G  I+ +          G K  SGTS+++PVV
Sbjct: 467 VAAFSSRGPNSVSP----AILKPDIAAPGVSILAAVSPLDPGAFNGFKLHSGTSMSTPVV 526

Query: 127 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKL--AGPNMYEQGAGR 169
           +G++ LL S+ P+       +PA+M+ ALV  A +   +G  ++ QG+ +
Sbjct: 527 SGIIVLLKSLHPK------WSPAAMRSALVTTAWRTSPSGEPIFAQGSNK 566

BLAST of CsGy1G016630 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 44.7 bits (104), Expect = 3.9e-04
Identity = 35/110 (31.82%), Postives = 59/110 (53.64%), Query Frame = 0

Query: 67  IASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKIST------GCKSLSGTSVASPVV 126
           +ASFSSRG      P     +KPD+ A G  I+ +  +       G   LSGTS+A+P +
Sbjct: 494 VASFSSRGPN----PISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTI 553

Query: 127 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKL--AGPNMYEQGAGR 169
           +G+V LL ++ P+       +PA+++ A+V  A +    G  ++ +G+ R
Sbjct: 554 SGIVALLKALHPD------WSPAAIRSAIVTTAWRTDPFGEQIFAEGSPR 593

BLAST of CsGy1G016630 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.6e-04
Identity = 34/110 (30.91%), Postives = 57/110 (51.82%), Query Frame = 0

Query: 67  IASFSSRGMTTWEMPHGYGRVKPDVVAYGREIMGSKIST------GCKSLSGTSVASPVV 126
           + +FSSRG  +         +KPD+ A G  I+ +          G   LSGTS+A+PV+
Sbjct: 506 VVNFSSRGPNSMSP----AILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVI 565

Query: 127 AGVVCLLVSVIPESQRKVILNPASMKQALVEGAAKL--AGPNMYEQGAGR 169
           +GV+ LL ++ PE       +PA+ + A+V  A +    G  ++ +G+ R
Sbjct: 566 SGVIALLKALHPE------WSPAAFRSAIVTTAWRTDPFGEQIFAEGSSR 605

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WUG60.0e+0079.65Subtilisin-like protease SBT6.1 OS=Arabidopsis thaliana OX=3702 GN=SBT6.1 PE=1 S... [more]
Q147035.1e-20353.28Membrane-bound transcription factor site-1 protease OS=Homo sapiens OX=9606 GN=M... [more]
Q9WTZ34.3e-20254.85Membrane-bound transcription factor site-1 protease OS=Rattus norvegicus OX=1011... [more]
Q9Z2A87.4e-20254.44Membrane-bound transcription factor site-1 protease OS=Cricetulus griseus OX=100... [more]
Q9WTZ21.1e-20054.44Membrane-bound transcription factor site-1 protease OS=Mus musculus OX=10090 GN=... [more]
Match NameE-valueIdentityDescription
KGN65332.20.099.87hypothetical protein Csa_017774 [Cucumis sativus][more]
XP_004150051.10.099.87subtilisin-like protease SBT6.1 [Cucumis sativus][more]
XP_008463395.10.098.25PREDICTED: subtilisin-like protease SBT6.1 [Cucumis melo][more]
XP_038879333.10.097.04subtilisin-like protease SBT6.1 [Benincasa hispida][more]
KAG6589917.10.095.70Subtilisin-like protease 6.1, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
A0A0A0LTP50.099.87Peptidase_S8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G25016... [more]
A0A1S3CJL60.098.25subtilisin-like protease SBT6.1 OS=Cucumis melo OX=3656 GN=LOC103501563 PE=3 SV=... [more]
A0A6J1H8D80.095.56subtilisin-like protease SBT6.1 OS=Cucurbita moschata OX=3662 GN=LOC111461434 PE... [more]
A0A6J1JAS50.095.56subtilisin-like protease SBT6.1 OS=Cucurbita maxima OX=3661 GN=LOC111485106 PE=3... [more]
A0A5A7TUN60.098.18Subtilisin-like protease SBT6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
Match NameE-valueIdentityDescription
AT5G19660.10.0e+0079.65SITE-1 protease [more]
AT4G10520.15.9e-0530.32Subtilase family protein [more]
AT4G21640.13.9e-0432.73Subtilase family protein [more]
AT4G10510.13.9e-0431.82Subtilase family protein [more]
AT1G32960.16.6e-0430.91Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 1..185
e-value: 6.7E-38
score: 132.6
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 2..171
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 1..159
e-value: 5.9E-25
score: 88.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 657..679
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 658..679
NoneNo IPR availablePANTHERPTHR43806:SF44MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASEcoord: 495..657
NoneNo IPR availablePANTHERPTHR43806:SF44MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-1 PROTEASEcoord: 1..466
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 495..657
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 1..466
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 1..178
score: 20.439335
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 112..122

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G016630.2CsGy1G016630.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0006508 proteolysis
biological_process GO:0007165 signal transduction
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0043531 ADP binding
molecular_function GO:0003953 NAD+ nucleosidase activity
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity