CsGy1G015000 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G015000
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionsubtilisin-like protease SBT5.4
LocationGy14Chr1: 10893883 .. 10898102 (-)
RNA-Seq ExpressionCsGy1G015000
SyntenyCsGy1G015000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGGTAATCAAAATATTAGAAAAGAGATTGTTTTTAAACAAGTCTTGAAGTTGTTTTATTATGCATGCATTATCTAATATTTATACCCATTTTGTTCTTTACTATTAAAAAAGATATATTGATTTGGTTTGGTTCATTAGATTGTTTTTTGGGTTGTGATTTTTTTCTTCCCAAAGCCCTAAAAGTTAAAATTGTAGTTTTCCTGATCTTTATTATTTGTTTTTTTTTTTATACATTGATTGATTCAGTCTATACTTTCTATGTTCATAAAGACATTATTAAACAAACCCCAAAACCCCAAAAATGGTTGGGACAAATTTCCTTTTCTTACTATATATGTGTATATAATTATCGATTAGGTTTGGTCAATATGAAGTGTTCTTGGGTTTTGAATTTTTTTTCTTCAGGTTTACAAAACCTCTTTTCTTTTTTTGGTTCTTGAACTCAGTTGATTCAGTGGTGTACTTAGTTTGTATGTATCTATGAATTATTAAAAATTATCTTAAAGATTGGATAGCAAAAACAATTAGCTCTATTTTGTAAAATAGAGCAAAATCTACTAAATTCTTTAGGTTCATTTAAGTTTTTTTTTTTTTTTTTTTTTGCATAATATAGTTTCAATTTTTGTTTATTTTGCTATCAATAAGAGTTTCTCCAAAACTAAATGGTTTTTTTTTTTGGTTTAATGTTTGGATTTCAGTCTTATATTGTGTTATTGGGATCCCATTCACATGGATTAGAAGTTACAGATGAAGATCTTAAAAGGGTGGCTGATTCCCATCACAAGTTGCTTGGATCCATCTTTGGAAGGTTTGAAATTTTATCTATAATTTCCTCATCATAACAACTTATTTTTCATTTGATTACTTTTTTTTTTAGAAAAAAAATCTTGTTGTTGTTATTATTATAATTCTTACTTATTAAATTTTAATTATTATAAAATTTGAAAATTTCAGTGATGAGAAGGCAAGAAATGCTATATTTTACTCCTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGACGAGGAAGAGGCAGCCCAGCTTGCAAGTAAAAAAATCAACTCATATACTTCTTTTAAAAAGAAATCTATTTTCACATCTTTTAGCATAACCAAGGTTTAATTTAAAAAACAAAAAGAATTATAATAATAGAAACTAAATTGGAAAATTATAAAAAATGTGACAGAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAGTCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTACACTTCTTTTTTTCTATTTTAATTAATATAGCAAAGATTTTATTCTTAGTATTTTACAAATTTAATTAATATAGCATAAATGATTGGAACAAATATGAAAACATAGGATAAAGACTTTGATGGAAGTAAAGTTTAGTTTATGGTCCAAATTAAAATAAATGTATATATTTGTTATCTTTTCTGTTTTGGGAAAAGGTGTGTGGCCGGAGTCCAAGAGTTTTGGAGAACATGGGATAGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCTTGATAGAGTGCCATGTAACAGGTTTGATGTTTTTGACACGAAGAGTTAATTAACTACTTTGATATTGATATTGATATTGATTTAAGGTTGATTATTTTTCTTTAATATTAGATTGATTTTAAAATAAATAAATATAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTCCTCGCATACTTGAAGTCTGAGAATTTGACAGCATTGGTGATTAACTCCACTCGTGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCTGGCGCCAGTGTGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCACGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCCAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTTCAGCATTCCATGCAGTGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTATGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGTTCGTCTCAGCTTTCACTCTCTAATTGAATGTGTGTATGAGCTCTATTTATATTTTGTTGGTTTTCTATATTCATTAGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCCATTAATAACTGGAGCTGAAGCGAAAGCCAAGAACGCCACCGCGGAGGTGGCCATGCTTTGCAAACCAAAAACATTGGATCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGACACTGCAAGAGTAGACAAAGGAGAACAAGCGGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAATGATGAGCTAAGTGGGTTCGAAACTATCGCAGATCCCCATGTTCTTCCTGCTTCCCATATCAATTACAATGATGGCCAAGCTGTTTTCTCTTACATCAAATCCACCAAGTAACTAATAATAACAAATTTTTTACCCTTTTCTAAAAGAAAAAAAACAAGTTGATTACGATGATATTTGGTTGTTTCTTTGGCAGGAATCCGATGGGGTATTTAATCCCACCAACGGCAAAAGTTAACACCAAACCTGCCCCCACTATGGCAGCCTTTTCATCTCGAGGACCCAACCTTATTTCCCCTGAGATTATCAAGGTTTCCTTTTTCTCAAACCCTTATTATGATTTTCTCTTAAAAATCTATTTTATACCTTAATGGATGTTAAGAATAGTTTGAGTCCTCAGAAAATTTTAATTAATATAATAAATTTAAGCTGATATGCTTTAGTTTTTGAGTTTTGATAACAGTTTAATATGATTGCAACATAAACAGTTCACAATATGACGAATCTTTTTAATGTTAAGCAGACATAGCAAACTCTTTCCCACTTAATGTATAGCTCAATAACATTAAAGTGTATTCGTTCAACGAATTTGTTTGATTTTAAATTTCTCATCTCCCCAAATAGTACTCAAAGAACTCACTCTAAAGTTATGACATATATTTTAGTTAAATGAATAAATGATTTAACTATAGAATCTCAGATCTCTCCGAATTAACGACTACTTGAATTAAAATTTTCAGCCAGATGTGACTGCACCGGGTGTGAATATAATTGCAGCATTCTCAGAGGCCGTAAGCCCAACAGGCGAACCATTTGACAATAGAACAGTTCCATTCATAACAATGTCAGGGACCTCCATGTCTTGCCCCCATGTCTCTGGTCTTGTTGGCCTTCTTAGAACTCTCCACCCTCAGTGGAGCCCCTCCGCCATCAAATCTGCCATCATGACCTCTGCCAGAATTCGTGACAACACAAAAAAACCCATGCTCGATGGTGGCTCCCCTGACCTCGCCCCTTCCACCCCTTTTGCCTATGGCTCTGGCCACATTCGCCCTACTGGAGCCATTGACCCCGGCCTCGTCTATGATCTCTCCCCCAATGACTACTTGGAATTCCTCTGCGCCAGTGGCTACAACGAGAAGACCATTCAAGCATTCTCTGATGGTCCTTTCAAATGTCCAGCTTCTGCTTCTATTTTAAACTTGAATTATCCTTCCATTGGGGTTCAGAATCTGACTGGCAGTGTCACCGTCACTAGGAAATTGAAGAACGTTAGCACTCCTGGGGTATACAAAGGCAGAGTTAGGCACCCCAATGGAGTCAAGGTTTTAGTGAAGCCCAAAGTTCTAAAGTTCGAGAGAGTTGGAGAGGAGAAAAGCTTTGAATTGACAATAACTGGAGATGTGCCAGAGGATCAGGTTGTTGACGGGGTGCTGATTTGGACTGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCGTTTCCTCTAGCTTGTTCTGA

mRNA sequence

ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGTCTTATATTGTGTTATTGGGATCCCATTCACATGGATTAGAAGTTACAGATGAAGATCTTAAAAGGGTGGCTGATTCCCATCACAAGTTGCTTGGATCCATCTTTGGAAGTGATGAGAAGGCAAGAAATGCTATATTTTACTCCTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGACGAGGAAGAGGCAGCCCAGCTTGCAAAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAGTCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTGTGTGGCCGGAGTCCAAGAGTTTTGGAGAACATGGGATAGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCTTGATAGAGTGCCATGTAACAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTCCTCGCATACTTGAAGTCTGAGAATTTGACAGCATTGGTGATTAACTCCACTCGTGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCTGGCGCCAGTGTGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCACGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCCAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTTCAGCATTCCATGCAGTGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTATGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCCATTAATAACTGGAGCTGAAGCGAAAGCCAAGAACGCCACCGCGGAGGTGGCCATGCTTTGCAAACCAAAAACATTGGATCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGACACTGCAAGAGTAGACAAAGGAGAACAAGCGGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAATGATGAGCTAAGTGGGTTCGAAACTATCGCAGATCCCCATGTTCTTCCTGCTTCCCATATCAATTACAATGATGGCCAAGCTGTTTTCTCTTACATCAAATCCACCAAGAATCCGATGGGGTATTTAATCCCACCAACGGCAAAAGTTAACACCAAACCTGCCCCCACTATGGCAGCCTTTTCATCTCGAGGACCCAACCTTATTTCCCCTGAGATTATCAAGCCAGATGTGACTGCACCGGGTGTGAATATAATTGCAGCATTCTCAGAGGCCGTAAGCCCAACAGGCGAACCATTTGACAATAGAACAGTTCCATTCATAACAATGTCAGGGACCTCCATGTCTTGCCCCCATGTCTCTGGTCTTGTTGGCCTTCTTAGAACTCTCCACCCTCAGTGGAGCCCCTCCGCCATCAAATCTGCCATCATGACCTCTGCCAGAATTCGTGACAACACAAAAAAACCCATGCTCGATGGTGGCTCCCCTGACCTCGCCCCTTCCACCCCTTTTGCCTATGGCTCTGGCCACATTCGCCCTACTGGAGCCATTGACCCCGGCCTCGTCTATGATCTCTCCCCCAATGACTACTTGGAATTCCTCTGCGCCAGTGGCTACAACGAGAAGACCATTCAAGCATTCTCTGATGGTCCTTTCAAATGTCCAGCTTCTGCTTCTATTTTAAACTTGAATTATCCTTCCATTGGGGTTCAGAATCTGACTGGCAGTGTCACCGTCACTAGGAAATTGAAGAACGTTAGCACTCCTGGGGTATACAAAGGCAGAGTTAGGCACCCCAATGGAGTCAAGGTTTTAGTGAAGCCCAAAGTTCTAAAGTTCGAGAGAGTTGGAGAGGAGAAAAGCTTTGAATTGACAATAACTGGAGATGTGCCAGAGGATCAGGTTGTTGACGGGGTGCTGATTTGGACTGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCGTTTCCTCTAGCTTGTTCTGA

Coding sequence (CDS)

ATGAAGCATCGGAAGATGAAGATCTATAATTCCATTCTCATTTTATTTTCACTCCTTTTGATAATATCACCTGCCATGGCTGCCAAAAAGTCTTATATTGTGTTATTGGGATCCCATTCACATGGATTAGAAGTTACAGATGAAGATCTTAAAAGGGTGGCTGATTCCCATCACAAGTTGCTTGGATCCATCTTTGGAAGTGATGAGAAGGCAAGAAATGCTATATTTTACTCCTATAAGAAGAATATAAATGGTTTTGCAGCAATTATGGACGAGGAAGAGGCAGCCCAGCTTGCAAAACACCCGGAAGTAGCAGCAGTATTGCCAAACAGAGCAAAAAAGTTACACACAACACATTCATGGGAGTTTATGCATTTGGAGAAGAATGGTGTAATTCCCCCTTCATCTGCTTGGCGTAGGGCTAAGTCTGGAAAAGACGTCATTATCGCCAATCTTGACACTGGTGTGTGGCCGGAGTCCAAGAGTTTTGGAGAACATGGGATAGTTGGACCTGTTCCATCAAAGTGGAAAGGAGGTTGCACTGATAAAACCCTTGATAGAGTGCCATGTAACAGGAAACTAATAGGAGCAAAGTACTTCAACAAGGGCTTCCTCGCATACTTGAAGTCTGAGAATTTGACAGCATTGGTGATTAACTCCACTCGTGACTACGACGGTCATGGTTCACACACCTTGTCCACGGCTGGGGGCAGCTATGTGTCTGGCGCCAGTGTGTTCGGGTTAGGCGTTGGAACTGCCAAAGGGGGCTCTCCCAAGGCACGTGTTGCTGCCTATAAGGTTTGCTGGCCACTTGAGGATGGAGGTTGTTTCGACGCTGACATTGCCCAGGCATTTGACCATGCCATTCACGACCGCGTTGATGTTCTTTCGCTCTCCCTTGGTGGCGAGCCAGCCGATTATTATGATGATGGAATCGCCATTTCAGCATTCCATGCAGTGAAGAAGGGAATCCCTGTGGTGTGCTCGGCTGGTAACTCTGGCCCTGGTGCTCAGACTGTTTCTAATACTGCACCTTGGATTTTGACTGTTGGTGCAAGTACTATGGATCGTGAGTTTCAGGCTCCTGTTGAGCTCCAAAATGGTCACCGTTATATGGGTTCGAGCCTTTCAAAAGGATTAAAGGGAGACAAGCTTTATCCATTAATAACTGGAGCTGAAGCGAAAGCCAAGAACGCCACCGCGGAGGTGGCCATGCTTTGCAAACCAAAAACATTGGATCATTCAAAAGTGAAAGGGAAGATATTGGTTTGCTTGAGAGGAGACACTGCAAGAGTAGACAAAGGAGAACAAGCGGCCCTTGCCGGTGCTGTCGGAATGATTCTATGCAATGATGAGCTAAGTGGGTTCGAAACTATCGCAGATCCCCATGTTCTTCCTGCTTCCCATATCAATTACAATGATGGCCAAGCTGTTTTCTCTTACATCAAATCCACCAAGAATCCGATGGGGTATTTAATCCCACCAACGGCAAAAGTTAACACCAAACCTGCCCCCACTATGGCAGCCTTTTCATCTCGAGGACCCAACCTTATTTCCCCTGAGATTATCAAGCCAGATGTGACTGCACCGGGTGTGAATATAATTGCAGCATTCTCAGAGGCCGTAAGCCCAACAGGCGAACCATTTGACAATAGAACAGTTCCATTCATAACAATGTCAGGGACCTCCATGTCTTGCCCCCATGTCTCTGGTCTTGTTGGCCTTCTTAGAACTCTCCACCCTCAGTGGAGCCCCTCCGCCATCAAATCTGCCATCATGACCTCTGCCAGAATTCGTGACAACACAAAAAAACCCATGCTCGATGGTGGCTCCCCTGACCTCGCCCCTTCCACCCCTTTTGCCTATGGCTCTGGCCACATTCGCCCTACTGGAGCCATTGACCCCGGCCTCGTCTATGATCTCTCCCCCAATGACTACTTGGAATTCCTCTGCGCCAGTGGCTACAACGAGAAGACCATTCAAGCATTCTCTGATGGTCCTTTCAAATGTCCAGCTTCTGCTTCTATTTTAAACTTGAATTATCCTTCCATTGGGGTTCAGAATCTGACTGGCAGTGTCACCGTCACTAGGAAATTGAAGAACGTTAGCACTCCTGGGGTATACAAAGGCAGAGTTAGGCACCCCAATGGAGTCAAGGTTTTAGTGAAGCCCAAAGTTCTAAAGTTCGAGAGAGTTGGAGAGGAGAAAAGCTTTGAATTGACAATAACTGGAGATGTGCCAGAGGATCAGGTTGTTGACGGGGTGCTGATTTGGACTGATGGCAAGCACTTTGTTAGGAGTCCCATTGTCGTTTCCTCTAGCTTGTTCTGA

Protein sequence

MKHRKMKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF*
Homology
BLAST of CsGy1G015000 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 5.2e-267
Identity = 471/777 (60.62%), Postives = 568/777 (73.10%), Query Frame = 0

Query: 5   KMKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSI 64
           KM + +   +L  + L  SPA A KKSYIV LGSH+H  +++   L  VA SH   L S 
Sbjct: 15  KMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASF 74

Query: 65  FGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFM 124
            GS E A+ AIFYSYK++INGFAAI+DE EAA++AKHP+V +V PN+ +KLHTTHSW FM
Sbjct: 75  VGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM 134

Query: 125 HLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT 184
            L KNGV+  SS W +A  G+D IIANLDTGVWPESKSF + G  G VP++WKG C    
Sbjct: 135 LLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD- 194

Query: 185 LDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINST----RDYDGHGSHTLSTAGGSYV 244
              VPCNRKLIGA+YFNKG+LAY      T L  N++    RD+DGHGSHTLSTA G++V
Sbjct: 195 ---VPCNRKLIGARYFNKGYLAY------TGLPSNASYETCRDHDGHGSHTLSTAAGNFV 254

Query: 245 SGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLS 304
            GA+VFG+G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S
Sbjct: 255 PGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSAS 314

Query: 305 LGGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREF 364
           +GG+  DY  DGIAI +FHAVK G+ VVCSAGNSGP + TVSN APW++TVGAS+MDREF
Sbjct: 315 VGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREF 374

Query: 365 QAPVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKG 424
           QA VEL+NG  + G+SLSK L  +K+Y LI+ A+A   N     A+LCK  +LD  KVKG
Sbjct: 375 QAFVELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKG 434

Query: 425 KILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVF 484
           KILVCLRGD ARVDKG QAA AGA GM+LCND+ SG E I+D HVLPAS I+Y DG+ +F
Sbjct: 435 KILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLF 494

Query: 485 SYIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFS 544
           SY+ STK+P GY+  PTA +NTKPAP MA+FSSRGPN I+P I+KPD+TAPGVNIIAAF+
Sbjct: 495 SYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFT 554

Query: 545 EAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIR 604
           EA  PT    DNR  PF T SGTSMSCPH+SG+VGLL+TLHP WSP+AI+SAIMT++R R
Sbjct: 555 EATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTR 614

Query: 605 DNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKT 664
           +N +KPM+D        + PF+YGSGH++P  A  PGLVYDL+  DYL+FLCA GYN   
Sbjct: 615 NNRRKPMVD---ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTV 674

Query: 665 IQAFSDGP-FKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGV 724
           +Q F++ P + C   A++L+ NYPSI V NLTGS+TVTRKLKNV  P  Y  R R P GV
Sbjct: 675 VQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGV 734

Query: 725 KVLVKPKVLKFERVGEEKSFELTITG-DVPEDQVVDGVLIWTDGKHFVRSPIVVSSS 775
           +V V+PK L F + GE K F++T+    V     V G L WTD  H+VRSPIVV  S
Sbjct: 735 RVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

BLAST of CsGy1G015000 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 1.0e-233
Identity = 418/771 (54.22%), Postives = 543/771 (70.43%), Query Frame = 0

Query: 11  SILILFSLLLIISPAMAAKK---SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGS 70
           S L+L  L+ + S  + A K   SY+V  G+HSH  E+T++ + RV ++H+  LGS  GS
Sbjct: 8   SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 67

Query: 71  DEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLE 130
            E+A +AIFYSY K+INGFAA +D + A +++KHPEV +V PN+A KLHTT SW+F+ LE
Sbjct: 68  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 127

Query: 131 KNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDR 190
            N  +P SS WR+A+ G+D IIANLDTGVWPESKSF + G+ GP+PS+WKG C ++    
Sbjct: 128 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDAT 187

Query: 191 VPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFG 250
             CNRKLIGA+YFNKG+ A +   N +    +S RD DGHGSHTLSTA G +V G S+FG
Sbjct: 188 FHCNRKLIGARYFNKGYAAAVGHLNSS---FDSPRDLDGHGSHTLSTAAGDFVPGVSIFG 247

Query: 251 LGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLSLGGEPAD 310
            G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+SLGGEP  
Sbjct: 248 QGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTS 307

Query: 311 YYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQ 370
           +++D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGASTMDREF + + L 
Sbjct: 308 FFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLG 367

Query: 371 NGHRYMGSSLSK-GLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL 430
           NG  Y G SLS   L   K YP++    AKAKNA+A  A LCK  +LD  K KGKILVCL
Sbjct: 368 NGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCL 427

Query: 431 RGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKST 490
           RG   RV+KG   AL G +GM+L N  ++G + +ADPHVLPA+ +   D  AV  YI  T
Sbjct: 428 RGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQT 487

Query: 491 KNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPT 550
           K P+ ++ P    +  KPAP MA+FSS+GP++++P+I+KPD+TAPGV++IAA++ AVSPT
Sbjct: 488 KKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPT 547

Query: 551 GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKP 610
            E FD R + F  +SGTSMSCPH+SG+ GLL+T +P WSP+AI+SAIMT+A I D+   P
Sbjct: 548 NEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGP 607

Query: 611 MLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSD 670
           + +  +     +TPF++G+GH++P  A++PGLVYDL   DYL FLC+ GYN   I  FS 
Sbjct: 608 IQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG 667

Query: 671 GPFKCPA-SASILNLNYPSIGVQNLTGS-VTVTRKLKNVSTPGVYKGRVRHPNGVKVLVK 730
             F C +   S++NLNYPSI V NLT S VTV+R +KNV  P +Y  +V +P GV V VK
Sbjct: 668 NNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVK 727

Query: 731 PKVLKFERVGEEKSFELTIT---GDVPEDQVVDGVLIWTDGKHFVRSPIVV 772
           P  L F +VGE+K+F++ +    G+V +  V  G L+W+D KH VRSPIVV
Sbjct: 728 PTSLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

BLAST of CsGy1G015000 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 663.3 bits (1710), Expect = 3.4e-189
Identity = 381/791 (48.17%), Postives = 500/791 (63.21%), Query Frame = 0

Query: 13  LILFSLLL---IISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDE 72
           LIL S  L   +++    +KK YIV +G+HSHG   T  DL+   DSH+ LLGSIFGS E
Sbjct: 10  LILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSRE 69

Query: 73  KARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKN 132
           KA+ AI YSY ++INGFAA+++EEEAA +AK+P V +V  ++  KLHTT SWEF+ L + 
Sbjct: 70  KAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR 129

Query: 133 GVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVP 192
           G    +SAW++ + G++ II N+DTGVWPES+SF + G  G VPSKW+GG     ++++P
Sbjct: 130 G---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQ--INKLP 189

Query: 193 ------CNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGA 252
                 CNRKLIGA+Y+NK F A+  +  L  L +++ RD+ GHG+HTLSTAGG++V GA
Sbjct: 190 GSMKNTCNRKLIGARYYNKAFEAH--NGQLDPL-LHTARDFVGHGTHTLSTAGGNFVPGA 249

Query: 253 SVFGLGVGTAKGGSPKARVAAYKVCWPLED-GGCFDADIAQAFDHAIHDRVDVLSLSLGG 312
            VF +G GTAKGGSP+ARVAAYKVCW L D   C+ AD+  A D AI D VDV+++S G 
Sbjct: 250 RVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGV 309

Query: 313 E----PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDRE 372
                    + D I+I AFHA+ K I +V SAGN GP   TV+N APW+ T+ AST+DR+
Sbjct: 310 SYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRD 369

Query: 373 FQAPVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVK 432
           F + + + N     G+SL   L  ++ + LI   +AK  NAT   A LC+  TLD +KV 
Sbjct: 370 FSSNLTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVN 429

Query: 433 GKILVCLR-GDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA 492
           GKI++C R G    V +G +A  AGA GMIL N   +G    A+PHV    +      ++
Sbjct: 430 GKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKS 489

Query: 493 VFSYIKSTKNPMGYLIPPTAKVNT------------KPAPTMAAFSSRGPNLISPEIIKP 552
               +K+T   +G    P    +T            KPAP MA+FSSRGPN I P I+KP
Sbjct: 490 RPHGVKTT--AIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKP 549

Query: 553 DVTAPGVNIIAAFSEAVSPTGEPFDNRT-VPFITMSGTSMSCPHVSGLVGLLRTLHPQWS 612
           DVTAPGVNI+AA+SE  S +    DNR    F  + GTSMSCPH SG+ GLL+T HP WS
Sbjct: 550 DVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWS 609

Query: 613 PSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPN 672
           P+AIKSAIMT+A   DNT +P+ D     LA +  FAYGSGH+RP  AI+PGLVYDLS  
Sbjct: 610 PAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLT 669

Query: 673 DYLEFLCASGYNEKTIQAFS-DGPFKCPASASILNLNYPSIGVQNL-TGSVTVTRKLKNV 732
           DYL FLCASGY+++ I A + +  F C  S S+ +LNYPSI + NL    VT+ R + NV
Sbjct: 670 DYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNV 729

Query: 733 STPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTI--TGDVPEDQVVDGVLIWTD 772
             P  Y    R PNG  + V P  L F ++GE K+F++ +  +      +   G L WTD
Sbjct: 730 GPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTD 786

BLAST of CsGy1G015000 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 3.1e-166
Identity = 332/706 (47.03%), Postives = 439/706 (62.18%), Query Frame = 0

Query: 75  IFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPP 134
           + Y+Y+  I+GF+  + +EEA  L   P V +VLP    +LHTT +  F+ L+++     
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 135 SSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT-LDRVPCNRK 194
           +  +  A S  DV++  LDTGVWPESKS+ + G  GP+PS WKGGC   T      CNRK
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLCNRK 184

Query: 195 LIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTA 254
           LIGA++F +G+ + +   +  +    S RD DGHG+HT STA GS V GAS+ G   GTA
Sbjct: 185 LIGARFFARGYESTMGPID-ESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 244

Query: 255 KGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIA 314
           +G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+SLGG  +DYY DG+A
Sbjct: 245 RGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 304

Query: 315 ISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMG 374
           I AF A+++GI V CSAGN+GP + ++SN APWI TVGA T+DR+F A   L NG  + G
Sbjct: 305 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 364

Query: 375 SSLSKG-LKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARV 434
            SL KG    DKL P I      A NAT     LC   TL   KVKGKI++C RG  ARV
Sbjct: 365 VSLFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARV 424

Query: 435 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYL 494
            KG+    AG VGMIL N   +G E +AD H+LPA+ +    G  +  Y+ +  NP   +
Sbjct: 425 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 484

Query: 495 IPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNR 554
                 V  KP+P +AAFSSRGPN I+P I+KPD+ APGVNI+AA++ A  PTG   D+R
Sbjct: 485 SILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 544

Query: 555 TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSP 614
            V F  +SGTSMSCPHVSGL  LL+++HP+WSP+AI+SA+MT+A       KP+LD  + 
Sbjct: 545 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 604

Query: 615 DLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPA 674
              PSTPF +G+GH+ PT A +PGL+YDL+  DYL FLCA  Y    I++ S   + C  
Sbjct: 605 --KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDP 664

Query: 675 SA--SILNLNYPSIGVQ-NLTGSVTVTRKLKNVSTPGVYKGRV-RHPNGVKVLVKPKVLK 734
           S   S+ +LNYPS  V  +  G+   TR + +V   G Y  +V     GVK+ V+P VL 
Sbjct: 665 SKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 724

Query: 735 FERVGEEKSFELTITGD--VPEDQVVDGVLIWTDGKHFVRSPIVVS 773
           F+   E+KS+ +T T D   P      G + W+DGKH V SP+ +S
Sbjct: 725 FKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CsGy1G015000 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 9.9e-157
Identity = 325/780 (41.67%), Postives = 453/780 (58.08%), Query Frame = 0

Query: 12  ILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKA 71
           +L+  S   I   A  A K++I  +   S         +  +  +H+    + F  + + 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGS---------MPSIFPTHYHWYSTEFAEESR- 67

Query: 72  RNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGV 131
              I + Y    +GF+A++  +EA  L  HP V AV  +R ++LHTT S +F+ L+    
Sbjct: 68  ---IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ-- 127

Query: 132 IPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDRVPC 191
                 W  +  G DVII   DTG+WPE +SF +  + GP+P +W+G C +        C
Sbjct: 128 ---KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFSPRNC 187

Query: 192 NRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGV 251
           NRK+IGA++F KG  A +       +   S RD DGHG+HT STA G +   AS+ G   
Sbjct: 188 NRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYAS 247

Query: 252 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGE---PADY 311
           G AKG +PKAR+AAYKVCW  +D GC D+DI  AFD A+ D VDV+S+S+GG     + Y
Sbjct: 248 GVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPY 307

Query: 312 YDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQN 371
           Y D IAI ++ A  KGI V  SAGN GP   +V+N APW+ TVGAST+DR F A   L +
Sbjct: 308 YLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGD 367

Query: 372 GHRYMGSSLSKGLK-GDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLR 431
           GHR  G SL  G+    +++P++   ++   +A+     LC   TLD  +V+GKI++C R
Sbjct: 368 GHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICDR 427

Query: 432 GDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTK 491
           G + RV KG     AG VGMIL N   +G   + D H++PA  +  N+G  + +Y  S  
Sbjct: 428 GSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHP 487

Query: 492 NPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTG 551
           NP+  +      V  KPAP +A+FS RGPN +SPEI+KPD+ APGVNI+AA+++AV PTG
Sbjct: 488 NPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTG 547

Query: 552 EPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPM 611
            P D R   F  +SGTSM+CPHVSG   LL++ HP WSP+ I+SA+MT+  + DN+ + +
Sbjct: 548 LPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSL 607

Query: 612 LDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDG 671
           +D  +   A  TP+ YGSGH+    A++PGLVYD++ +DY+ FLC+ GY  KTIQ  +  
Sbjct: 608 IDESTGKSA--TPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRT 667

Query: 672 PFKCPAS--ASILNLNYPSIGV---QNLTG--SVTVTRKLKNV-STPGVYKGRVRHPNGV 731
           P +CP +   S  NLNYPSI      N  G  S TV R   NV     VY+ R+  P GV
Sbjct: 668 PVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGV 727

Query: 732 KVLVKPKVLKFERVGEEKSFELTITGD-----VPEDQVVDGVLIWTD-GKHFVRSPIVVS 773
            V VKP  L F    + +S+ +T+T +     + E   V G + W D GKH VRSPIVV+
Sbjct: 728 TVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of CsGy1G015000 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 1501 bits (3887), Expect = 0.0
Identity = 748/753 (99.34%), Postives = 749/753 (99.47%), Query Frame = 0

Query: 26  MAAKK--SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNI 85
           MAAKK  SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNI
Sbjct: 1   MAAKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNI 60

Query: 86  NGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKS 145
           NGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKS
Sbjct: 61  NGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKS 120

Query: 146 GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKG 205
           GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKG
Sbjct: 121 GKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKG 180

Query: 206 FLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVA 265
           FLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVA
Sbjct: 181 FLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVA 240

Query: 266 AYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKG 325
           AYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKG
Sbjct: 241 AYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKG 300

Query: 326 IPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGD 385
           IPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGD
Sbjct: 301 IPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGD 360

Query: 386 KLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGA 445
           KLYPLITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGA
Sbjct: 361 KLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGA 420

Query: 446 VGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYLIPPTAKVNTKP 505
           VGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIK+TKNPMGYLIPPTAKVNTKP
Sbjct: 421 VGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKP 480

Query: 506 APTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTS 565
           APTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTS
Sbjct: 481 APTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTS 540

Query: 566 MSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYG 625
           MSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYG
Sbjct: 541 MSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYG 600

Query: 626 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPS 685
           SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPS
Sbjct: 601 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPS 660

Query: 686 IGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTIT 745
           IGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTIT
Sbjct: 661 IGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTIT 720

Query: 746 GDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           GDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 721 GDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1498 bits (3879), Expect = 0.0
Identity = 744/751 (99.07%), Postives = 746/751 (99.33%), Query Frame = 0

Query: 26  MAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING 85
           M   +SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING
Sbjct: 7   MLGFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING 66

Query: 86  FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 145
           FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK
Sbjct: 67  FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 126

Query: 146 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL 205
           DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL
Sbjct: 127 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL 186

Query: 206 AYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY 265
           AYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY
Sbjct: 187 AYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY 246

Query: 266 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 325
           KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP
Sbjct: 247 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 306

Query: 326 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 385
           VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL
Sbjct: 307 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 366

Query: 386 YPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 445
           YPLITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG
Sbjct: 367 YPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 426

Query: 446 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYLIPPTAKVNTKPAP 505
           MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIK+TKNPMGYLIPPTAKVNTKPAP
Sbjct: 427 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAP 486

Query: 506 TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 565
           TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS
Sbjct: 487 TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 546

Query: 566 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 625
           CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG
Sbjct: 547 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 606

Query: 626 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG 685
           HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG
Sbjct: 607 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG 666

Query: 686 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 745
           VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD
Sbjct: 667 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 726

Query: 746 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 727 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 1475 bits (3819), Expect = 0.0
Identity = 735/776 (94.72%), Postives = 753/776 (97.04%), Query Frame = 0

Query: 1   MKHRKMKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKL 60
           MKH KMKIYNSILILFSLLL+ISPA+AAKKSYIVLLGSHSHGLEV DEDL+RVADSHHKL
Sbjct: 1   MKHPKMKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKL 60

Query: 61  LGSIFGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHS 120
           LGSI GSDEKAR+AIFYSYK+NINGFAAIMDEEEA QLAKHPEVAAVL N+AKKLHTTHS
Sbjct: 61  LGSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHS 120

Query: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
           WEFMHLEKNGVIPPSSAWRRAKSGKDVII NLDTGVW ESKSFGEHGIVG VPSKWKGGC
Sbjct: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGC 180

Query: 181 TDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYV 240
           TDKT D V CNRKLIGAKYFNKGFLAYL S+NLTA VINSTRDYDGHGSHTLSTAGGSYV
Sbjct: 181 TDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGSHTLSTAGGSYV 240

Query: 241 SGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSL 300
           SGASVFGLGVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIA+AFDHAIHDRVDVLSLSL
Sbjct: 241 SGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSL 300

Query: 301 GGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQ 360
           GGEPADYYDDGIAI+AFHAVKKGIPVVCSAGNSGP AQTVSNTAPWILTVGAST+DREFQ
Sbjct: 301 GGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQ 360

Query: 361 APVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK 420
           APVELQNGH YMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK
Sbjct: 361 APVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK 420

Query: 421 ILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFS 480
           ILVCLRGDTARVDKGEQAALAGAVGMILCND+LSGFETIADPHVLPASHINYNDGQAVFS
Sbjct: 421 ILVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFS 480

Query: 481 YIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSE 540
           YIKSTKNPMG LIPP+AKVNTKPAP+MAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSE
Sbjct: 481 YIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSE 540

Query: 541 AVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRD 600
           AVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRD
Sbjct: 541 AVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRD 600

Query: 601 NTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTI 660
           NTKKPMLDGGSPDLAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTI
Sbjct: 601 NTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTI 660

Query: 661 QAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKV 720
           QAFSDGPFKCPASASILN NYPSIGVQNLTGSVT+TRKLKNVSTPGVYK RV HPNGVKV
Sbjct: 661 QAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKV 720

Query: 721 LVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           LVKPKVLKFERVGEEK FEL ITGDVPE+QVVDGVLIWTDGKHFVRSPIVVSS LF
Sbjct: 721 LVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1369 bits (3544), Expect = 0.0
Identity = 676/773 (87.45%), Postives = 722/773 (93.40%), Query Frame = 0

Query: 6   MKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIF 65
           MKI NSILILFS LL+IS A+AAKKSY+VLLGSHSHG+E+T+EDL+RV  SHHKLLGS  
Sbjct: 1   MKISNSILILFSFLLLISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFL 60

Query: 66  GSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMH 125
           GS+EKA++AIFYSYKK+INGFAA +DEEEA +LA+HPEVAA+L NRAK LHTTHSWEFMH
Sbjct: 61  GSEEKAKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMH 120

Query: 126 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTL 185
           LEKNGVIP SS WR AKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC D+T 
Sbjct: 121 LEKNGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETP 180

Query: 186 DRVPCNRKLIGAKYFNKGFLAYLKSENLTAL--VINSTRDYDGHGSHTLSTAGGSYVSGA 245
           DRVPCNRKLIGAKYFNKGF+AYLKSEN T    VINSTRDY+GHGSHTLSTAGGSYVSGA
Sbjct: 181 DRVPCNRKLIGAKYFNKGFIAYLKSENSTEFSSVINSTRDYEGHGSHTLSTAGGSYVSGA 240

Query: 246 SVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGE 305
           SVFG G GTAKGGSPKARVAAYKVCWPLE GGCFD+DI +AFDHAIHD VDVLSLSLG +
Sbjct: 241 SVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSD 300

Query: 306 PADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPV 365
           PA+Y +D IAI+AFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DREFQAPV
Sbjct: 301 PAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPV 360

Query: 366 ELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILV 425
           ELQNGHRYMGSSLSK L+G+KLYPLITGA+AKAKNA  + AMLCKP+TLDHSKVKGKIL 
Sbjct: 361 ELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILA 420

Query: 426 CLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIK 485
           CLRGD ARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAV SYI 
Sbjct: 421 CLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYIN 480

Query: 486 STKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVS 545
           STKNPMGYLIPP+AKVNTKPAPTMAAFSSRGPN+ISPEIIKPDVTAPGVN+IAAFSEAVS
Sbjct: 481 STKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVS 540

Query: 546 PTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTK 605
           PTGE FDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRDNT 
Sbjct: 541 PTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTM 600

Query: 606 KPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAF 665
           KPMLDGGSPDLAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY++KTI+AF
Sbjct: 601 KPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAF 660

Query: 666 SDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVK 725
           SDGPFKCP +ASILN NYPSIGVQNL G+VTVTRKLKNVSTPGVY+ R+ +P+GVKV VK
Sbjct: 661 SDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVK 720

Query: 726 PKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           PKVLKFERV EEKSFELT+TG+VPEDQVVDGVLIWTDGKHFVRSPIV+SS LF
Sbjct: 721 PKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 773

BLAST of CsGy1G015000 vs. NCBI nr
Match: XP_022142562.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 1266 bits (3275), Expect = 0.0
Identity = 616/779 (79.08%), Postives = 697/779 (89.47%), Query Frame = 0

Query: 6   MKIYNSILILFSLLLI----ISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLL 65
           MK+ NSIL +FS LL+    ISPA+AAKKSY+V+LGSHSHGLEV++ DL+RV DSHHKLL
Sbjct: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60

Query: 66  GSIFGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSW 125
           GS  GS EKA++AIFYSYKKNINGFAAI++EEEAA+LAKHPEVAAVL N+ KKLHTTHSW
Sbjct: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120

Query: 126 EFMHLEK-NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 185
            FM LEK NGV+PP+S W RA  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGC
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180

Query: 186 TDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTA---LVINSTRDYDGHGSHTLSTAGG 245
           TD + DRVPCNRKLIGAKYFNKGF+AYLK+ N +A    + NSTRDY+GHGSHTLSTAGG
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240

Query: 246 SYVSGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLS 305
           S+V  ASVFG G+GTAKGGSPKARVAAYK+CWP +DGGCFDADI + FDHAIHD V+V+S
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300

Query: 306 LSLGGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDR 365
           LS+GG PA+Y+DD IAI+AFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360

Query: 366 EFQAPVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKV 425
           +FQAPVEL+NG R+ GSSLS  L  DKLYPLITGA+AKA+NA+A  AMLCKP+TLDHSK 
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420

Query: 426 KGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA 485
           KGKIL CLRGD ARVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480

Query: 486 VFSYIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAA 545
           V  YI+ST+NPMGYLIPP AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540

Query: 546 FSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSAR 605
           ++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSAR
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600

Query: 606 IRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNE 665
           IRDNT KPMLDGG+ +LAP+TPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+E
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660

Query: 666 KTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNG 725
           K I+AFSDGP+KCP SAS+LN NYPSIGVQN+TGSVTVTR+LKNV TPGVY+ RVR P G
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720

Query: 726 VKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           V+V V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of CsGy1G015000 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 1498 bits (3879), Expect = 0.0
Identity = 744/751 (99.07%), Postives = 746/751 (99.33%), Query Frame = 0

Query: 26  MAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING 85
           M   +SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING
Sbjct: 7   MLGFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING 66

Query: 86  FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 145
           FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK
Sbjct: 67  FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 126

Query: 146 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL 205
           DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL
Sbjct: 127 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL 186

Query: 206 AYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY 265
           AYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY
Sbjct: 187 AYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAY 246

Query: 266 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 325
           KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP
Sbjct: 247 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 306

Query: 326 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 385
           VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL
Sbjct: 307 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 366

Query: 386 YPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 445
           YPLITGAEAKAKNATAE A LCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG
Sbjct: 367 YPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 426

Query: 446 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYLIPPTAKVNTKPAP 505
           MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIK+TKNPMGYLIPPTAKVNTKPAP
Sbjct: 427 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAP 486

Query: 506 TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 565
           TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS
Sbjct: 487 TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 546

Query: 566 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 625
           CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG
Sbjct: 547 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 606

Query: 626 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG 685
           HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG
Sbjct: 607 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIG 666

Query: 686 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 745
           VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD
Sbjct: 667 VQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGD 726

Query: 746 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF
Sbjct: 727 VPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of CsGy1G015000 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 1475 bits (3819), Expect = 0.0
Identity = 735/776 (94.72%), Postives = 753/776 (97.04%), Query Frame = 0

Query: 1   MKHRKMKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKL 60
           MKH KMKIYNSILILFSLLL+ISPA+AAKKSYIVLLGSHSHGLEV DEDL+RVADSHHKL
Sbjct: 1   MKHPKMKIYNSILILFSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKL 60

Query: 61  LGSIFGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHS 120
           LGSI GSDEKAR+AIFYSYK+NINGFAAIMDEEEA QLAKHPEVAAVL N+AKKLHTTHS
Sbjct: 61  LGSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHS 120

Query: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180
           WEFMHLEKNGVIPPSSAWRRAKSGKDVII NLDTGVW ESKSFGEHGIVG VPSKWKGGC
Sbjct: 121 WEFMHLEKNGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGC 180

Query: 181 TDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYV 240
           TDKT D V CNRKLIGAKYFNKGFLAYL S+NLTA VINSTRDYDGHGSHTLSTAGGSYV
Sbjct: 181 TDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTASVINSTRDYDGHGSHTLSTAGGSYV 240

Query: 241 SGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSL 300
           SGASVFGLGVGTAKGGSPKARVA+YKVCWPLEDGGCF+ADIA+AFDHAIHDRVDVLSLSL
Sbjct: 241 SGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSL 300

Query: 301 GGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQ 360
           GGEPADYYDDGIAI+AFHAVKKGIPVVCSAGNSGP AQTVSNTAPWILTVGAST+DREFQ
Sbjct: 301 GGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQ 360

Query: 361 APVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK 420
           APVELQNGH YMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK
Sbjct: 361 APVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGK 420

Query: 421 ILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFS 480
           ILVCLRGDTARVDKGEQAALAGAVGMILCND+LSGFETIADPHVLPASHINYNDGQAVFS
Sbjct: 421 ILVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFS 480

Query: 481 YIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSE 540
           YIKSTKNPMG LIPP+AKVNTKPAP+MAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSE
Sbjct: 481 YIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSE 540

Query: 541 AVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRD 600
           AVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHP WSPSAIKSAIMTSARIRD
Sbjct: 541 AVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRD 600

Query: 601 NTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTI 660
           NTKKPMLDGGSPDLAP+TPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTI
Sbjct: 601 NTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTI 660

Query: 661 QAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKV 720
           QAFSDGPFKCPASASILN NYPSIGVQNLTGSVT+TRKLKNVSTPGVYK RV HPNGVKV
Sbjct: 661 QAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKV 720

Query: 721 LVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           LVKPKVLKFERVGEEK FEL ITGDVPE+QVVDGVLIWTDGKHFVRSPIVVSS LF
Sbjct: 721 LVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of CsGy1G015000 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 1266 bits (3275), Expect = 0.0
Identity = 616/779 (79.08%), Postives = 697/779 (89.47%), Query Frame = 0

Query: 6   MKIYNSILILFSLLLI----ISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLL 65
           MK+ NSIL +FS LL+    ISPA+AAKKSY+V+LGSHSHGLEV++ DL+RV DSHHKLL
Sbjct: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60

Query: 66  GSIFGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSW 125
           GS  GS EKA++AIFYSYKKNINGFAAI++EEEAA+LAKHPEVAAVL N+ KKLHTTHSW
Sbjct: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120

Query: 126 EFMHLEK-NGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 185
            FM LEK NGV+PP+S W RA  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGC
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180

Query: 186 TDKTLDRVPCNRKLIGAKYFNKGFLAYLKSENLTA---LVINSTRDYDGHGSHTLSTAGG 245
           TD + DRVPCNRKLIGAKYFNKGF+AYLK+ N +A    + NSTRDY+GHGSHTLSTAGG
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240

Query: 246 SYVSGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLS 305
           S+V  ASVFG G+GTAKGGSPKARVAAYK+CWP +DGGCFDADI + FDHAIHD V+V+S
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300

Query: 306 LSLGGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDR 365
           LS+GG PA+Y+DD IAI+AFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360

Query: 366 EFQAPVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKV 425
           +FQAPVEL+NG R+ GSSLS  L  DKLYPLITGA+AKA+NA+A  AMLCKP+TLDHSK 
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420

Query: 426 KGKILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA 485
           KGKIL CLRGD ARVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480

Query: 486 VFSYIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAA 545
           V  YI+ST+NPMGYLIPP AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540

Query: 546 FSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSAR 605
           ++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSAR
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600

Query: 606 IRDNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNE 665
           IRDNT KPMLDGG+ +LAP+TPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+E
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660

Query: 666 KTIQAFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNG 725
           K I+AFSDGP+KCP SAS+LN NYPSIGVQN+TGSVTVTR+LKNV TPGVY+ RVR P G
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720

Query: 726 VKVLVKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           V+V V+P+ LKF++VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of CsGy1G015000 vs. ExPASy TrEMBL
Match: A0A0A0LYF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1)

HSP 1 Score: 1230 bits (3183), Expect = 0.0
Identity = 607/775 (78.32%), Postives = 679/775 (87.61%), Query Frame = 0

Query: 6   MKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIF 65
           MK + S LI FS LL+ISPA+A KKSY+VLLGSHSHGL+ T++D KRV DSHHKLLGS  
Sbjct: 1   MKFFISPLIFFSFLLLISPAIATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFL 60

Query: 66  GSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMH 125
            S+EKA++AIFYSYKKNINGFAA +D+E+A +LA HPEVAAVLPN+AK L+TTHSWEFMH
Sbjct: 61  RSEEKAKDAIFYSYKKNINGFAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMH 120

Query: 126 LEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTD-KT 185
           LEKNGVIPPSS W RAK GKDVIIANLDTGVWPESKSFGEHGIVGP PSKWKGGCTD KT
Sbjct: 121 LEKNGVIPPSSPWWRAKFGKDVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKT 180

Query: 186 LDRVPCNRKLIGAKYFNKGFLAYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVS 245
            D VPCN+KLIGAKYFNKG+  YLKSEN T   + +INSTRDY+GHGSHTLSTAGG+YV 
Sbjct: 181 PDGVPCNQKLIGAKYFNKGYFEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVV 240

Query: 246 GASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLG 305
           GASVFG G+GTAKGGSPKARVAAYKVCWP E GGCFDADI +AFDHAIHD VDVLSLSLG
Sbjct: 241 GASVFGSGIGTAKGGSPKARVAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLG 300

Query: 306 GEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQA 365
            +   Y +D IAI++FHAVKKGIPVVC+ GNSGP  +T SNTAPWILTVGAST+DREF A
Sbjct: 301 SDAIKYSEDAIAIASFHAVKKGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYA 360

Query: 366 PVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKI 425
           PV L+NG+++MGSS SKGL+G  LYPLITGA+AKA NAT + AMLCKP+TLDHSKVKGKI
Sbjct: 361 PVVLRNGYKFMGSSHSKGLRGRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKI 420

Query: 426 LVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSY 485
           LVCLRG+TAR+DKG+QAALAGAVGMILCND+LSG     D HVLPASHINY+DGQ + SY
Sbjct: 421 LVCLRGETARLDKGKQAALAGAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSY 480

Query: 486 IKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEA 545
             S + PMG LIPP A+VNTKPAPTMA FSSRGPN ISPEIIKPDVTAPGV+IIAAFSEA
Sbjct: 481 TNSARYPMGCLIPPLARVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEA 540

Query: 546 VSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDN 605
           +SPT +P DNRT PFITMSGTSMSCPHV+GLVGLLR LHP W+PSAIKSAIMTSA++RDN
Sbjct: 541 ISPTRDPSDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDN 600

Query: 606 TKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQ 665
           T  PMLDGGS  L P+TPFAYGSGHI PTGA+DPGLVYDLSPNDYLEFLCASGY+E+TI+
Sbjct: 601 TLNPMLDGGSLGLDPATPFAYGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIR 660

Query: 666 AFSDGPFKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVL 725
           AFSD PFKCPASAS+LNLNYPSIGVQNL  SVT+TRKLKNV TPGVYK ++ HPN V+V 
Sbjct: 661 AFSDEPFKCPASASVLNLNYPSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVS 720

Query: 726 VKPKVLKFERVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
           VKP+ LKFERVGEEKSFELT++G VP+++   G LIW+DG+HFVRSPIVVSS LF
Sbjct: 721 VKPRFLKFERVGEEKSFELTVSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

BLAST of CsGy1G015000 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 1225 bits (3169), Expect = 0.0
Identity = 596/749 (79.57%), Postives = 662/749 (88.38%), Query Frame = 0

Query: 31   SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAIM 90
            SY+VLLGSHSHGLEVT++D + V DSHHKLLGS   SDEKA+++IFYSYKK+INGFAA +
Sbjct: 755  SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814

Query: 91   DEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIA 150
            D+E+A +LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK GKDVIIA
Sbjct: 815  DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 874

Query: 151  NLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLKS 210
            NLDTGVWPESKSFGEHGI GP PSKWKGGCTDK+ D VPCN+KLIGAKYFNKG+L YLKS
Sbjct: 875  NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 934

Query: 211  ENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAYKV 270
            EN T   + +INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKARVAAYKV
Sbjct: 935  ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 994

Query: 271  CWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIPVV 330
            CWP E GGCFDADI +AFDHAIHD VDVLSLSLGG+P  Y +D IAI++FHAVKKGIPVV
Sbjct: 995  CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 1054

Query: 331  CSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKLYP 390
            C+ GNSGP  +T SNTAPWILTVGAST+DREF APV LQNGHR+MGSS SKGL G KLYP
Sbjct: 1055 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 1114

Query: 391  LITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMI 450
            LITGA+AKA NA  + AMLCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVGMI
Sbjct: 1115 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 1174

Query: 451  LCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYLIPPTAKVNTKPAPTM 510
            LCND+LSG   + D H+LPASHINY DGQ + SYI S +NPMGYLIPP AKVNTKPAPTM
Sbjct: 1175 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 1234

Query: 511  AAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCP 570
            A FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT +  DNRT PFITMSGTSMSCP
Sbjct: 1235 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 1294

Query: 571  HVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHI 630
            HV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT  PM+DGGS DLAP+TPFAYGSGHI
Sbjct: 1295 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 1354

Query: 631  RPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQ 690
             PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LNLNYPSIGVQ
Sbjct: 1355 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 1414

Query: 691  NLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVP 750
            NL GSV+VTRKLKNV +PGVY+ ++ HPNGV V VKP+ LKFERVGEEKSFELT+ G VP
Sbjct: 1415 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 1474

Query: 751  EDQVVDGVLIWTDGKHFVRSPIVVSSSLF 776
            +D+V  G LIW+DG+H VRSPIVVSS LF
Sbjct: 1475 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503

BLAST of CsGy1G015000 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 921.8 bits (2381), Expect = 3.7e-268
Identity = 471/777 (60.62%), Postives = 568/777 (73.10%), Query Frame = 0

Query: 5   KMKIYNSILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSI 64
           KM + +   +L  + L  SPA A KKSYIV LGSH+H  +++   L  VA SH   L S 
Sbjct: 15  KMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASF 74

Query: 65  FGSDEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFM 124
            GS E A+ AIFYSYK++INGFAAI+DE EAA++AKHP+V +V PN+ +KLHTTHSW FM
Sbjct: 75  VGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM 134

Query: 125 HLEKNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT 184
            L KNGV+  SS W +A  G+D IIANLDTGVWPESKSF + G  G VP++WKG C    
Sbjct: 135 LLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD- 194

Query: 185 LDRVPCNRKLIGAKYFNKGFLAYLKSENLTALVINST----RDYDGHGSHTLSTAGGSYV 244
              VPCNRKLIGA+YFNKG+LAY      T L  N++    RD+DGHGSHTLSTA G++V
Sbjct: 195 ---VPCNRKLIGARYFNKGYLAY------TGLPSNASYETCRDHDGHGSHTLSTAAGNFV 254

Query: 245 SGASVFGLGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLS 304
            GA+VFG+G GTA GGSPKARVAAYKVCWP  DG  CFDADI  A + AI D VDVLS S
Sbjct: 255 PGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSAS 314

Query: 305 LGGEPADYYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREF 364
           +GG+  DY  DGIAI +FHAVK G+ VVCSAGNSGP + TVSN APW++TVGAS+MDREF
Sbjct: 315 VGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREF 374

Query: 365 QAPVELQNGHRYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKG 424
           QA VEL+NG  + G+SLSK L  +K+Y LI+ A+A   N     A+LCK  +LD  KVKG
Sbjct: 375 QAFVELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKG 434

Query: 425 KILVCLRGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVF 484
           KILVCLRGD ARVDKG QAA AGA GM+LCND+ SG E I+D HVLPAS I+Y DG+ +F
Sbjct: 435 KILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLF 494

Query: 485 SYIKSTKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFS 544
           SY+ STK+P GY+  PTA +NTKPAP MA+FSSRGPN I+P I+KPD+TAPGVNIIAAF+
Sbjct: 495 SYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFT 554

Query: 545 EAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIR 604
           EA  PT    DNR  PF T SGTSMSCPH+SG+VGLL+TLHP WSP+AI+SAIMT++R R
Sbjct: 555 EATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTR 614

Query: 605 DNTKKPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKT 664
           +N +KPM+D        + PF+YGSGH++P  A  PGLVYDL+  DYL+FLCA GYN   
Sbjct: 615 NNRRKPMVD---ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTV 674

Query: 665 IQAFSDGP-FKCPASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGV 724
           +Q F++ P + C   A++L+ NYPSI V NLTGS+TVTRKLKNV  P  Y  R R P GV
Sbjct: 675 VQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGV 734

Query: 725 KVLVKPKVLKFERVGEEKSFELTITG-DVPEDQVVDGVLIWTDGKHFVRSPIVVSSS 775
           +V V+PK L F + GE K F++T+    V     V G L WTD  H+VRSPIVV  S
Sbjct: 735 RVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

BLAST of CsGy1G015000 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 811.2 bits (2094), Expect = 7.1e-235
Identity = 418/771 (54.22%), Postives = 543/771 (70.43%), Query Frame = 0

Query: 11  SILILFSLLLIISPAMAAKK---SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGS 70
           S L+L  L+ + S  + A K   SY+V  G+HSH  E+T++ + RV ++H+  LGS  GS
Sbjct: 8   SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 67

Query: 71  DEKARNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLE 130
            E+A +AIFYSY K+INGFAA +D + A +++KHPEV +V PN+A KLHTT SW+F+ LE
Sbjct: 68  RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 127

Query: 131 KNGVIPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDR 190
            N  +P SS WR+A+ G+D IIANLDTGVWPESKSF + G+ GP+PS+WKG C ++    
Sbjct: 128 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDAT 187

Query: 191 VPCNRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFG 250
             CNRKLIGA+YFNKG+ A +   N +    +S RD DGHGSHTLSTA G +V G S+FG
Sbjct: 188 FHCNRKLIGARYFNKGYAAAVGHLNSS---FDSPRDLDGHGSHTLSTAAGDFVPGVSIFG 247

Query: 251 LGVGTAKGGSPKARVAAYKVCWPLEDGG-CFDADIAQAFDHAIHDRVDVLSLSLGGEPAD 310
            G GTAKGGSP+ARVAAYKVCWP   G  C+DAD+  AFD AIHD  DV+S+SLGGEP  
Sbjct: 248 QGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTS 307

Query: 311 YYDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQ 370
           +++D +AI +FHA KK I VVCSAGNSGP   TVSN APW +TVGASTMDREF + + L 
Sbjct: 308 FFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLG 367

Query: 371 NGHRYMGSSLSK-GLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCL 430
           NG  Y G SLS   L   K YP++    AKAKNA+A  A LCK  +LD  K KGKILVCL
Sbjct: 368 NGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCL 427

Query: 431 RGDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKST 490
           RG   RV+KG   AL G +GM+L N  ++G + +ADPHVLPA+ +   D  AV  YI  T
Sbjct: 428 RGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQT 487

Query: 491 KNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPT 550
           K P+ ++ P    +  KPAP MA+FSS+GP++++P+I+KPD+TAPGV++IAA++ AVSPT
Sbjct: 488 KKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPT 547

Query: 551 GEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKP 610
            E FD R + F  +SGTSMSCPH+SG+ GLL+T +P WSP+AI+SAIMT+A I D+   P
Sbjct: 548 NEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGP 607

Query: 611 MLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSD 670
           + +  +     +TPF++G+GH++P  A++PGLVYDL   DYL FLC+ GYN   I  FS 
Sbjct: 608 IQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG 667

Query: 671 GPFKCPA-SASILNLNYPSIGVQNLTGS-VTVTRKLKNVSTPGVYKGRVRHPNGVKVLVK 730
             F C +   S++NLNYPSI V NLT S VTV+R +KNV  P +Y  +V +P GV V VK
Sbjct: 668 NNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVK 727

Query: 731 PKVLKFERVGEEKSFELTIT---GDVPEDQVVDGVLIWTDGKHFVRSPIVV 772
           P  L F +VGE+K+F++ +    G+V +  V  G L+W+D KH VRSPIVV
Sbjct: 728 PTSLNFTKVGEQKTFKVILVKSKGNVAKGYVF-GELVWSDKKHRVRSPIVV 770

BLAST of CsGy1G015000 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 587.0 bits (1512), Expect = 2.2e-167
Identity = 332/706 (47.03%), Postives = 439/706 (62.18%), Query Frame = 0

Query: 75  IFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPP 134
           + Y+Y+  I+GF+  + +EEA  L   P V +VLP    +LHTT +  F+ L+++     
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 135 SSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKT-LDRVPCNRK 194
           +  +  A S  DV++  LDTGVWPESKS+ + G  GP+PS WKGGC   T      CNRK
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLCNRK 184

Query: 195 LIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTA 254
           LIGA++F +G+ + +   +  +    S RD DGHG+HT STA GS V GAS+ G   GTA
Sbjct: 185 LIGARFFARGYESTMGPID-ESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 244

Query: 255 KGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIA 314
           +G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+SLGG  +DYY DG+A
Sbjct: 245 RGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 304

Query: 315 ISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMG 374
           I AF A+++GI V CSAGN+GP + ++SN APWI TVGA T+DR+F A   L NG  + G
Sbjct: 305 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 364

Query: 375 SSLSKG-LKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARV 434
            SL KG    DKL P I      A NAT     LC   TL   KVKGKI++C RG  ARV
Sbjct: 365 VSLFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARV 424

Query: 435 DKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGYL 494
            KG+    AG VGMIL N   +G E +AD H+LPA+ +    G  +  Y+ +  NP   +
Sbjct: 425 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 484

Query: 495 IPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNR 554
                 V  KP+P +AAFSSRGPN I+P I+KPD+ APGVNI+AA++ A  PTG   D+R
Sbjct: 485 SILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 544

Query: 555 TVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSP 614
            V F  +SGTSMSCPHVSGL  LL+++HP+WSP+AI+SA+MT+A       KP+LD  + 
Sbjct: 545 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 604

Query: 615 DLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPA 674
              PSTPF +G+GH+ PT A +PGL+YDL+  DYL FLCA  Y    I++ S   + C  
Sbjct: 605 --KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDP 664

Query: 675 SA--SILNLNYPSIGVQ-NLTGSVTVTRKLKNVSTPGVYKGRV-RHPNGVKVLVKPKVLK 734
           S   S+ +LNYPS  V  +  G+   TR + +V   G Y  +V     GVK+ V+P VL 
Sbjct: 665 SKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 724

Query: 735 FERVGEEKSFELTITGD--VPEDQVVDGVLIWTDGKHFVRSPIVVS 773
           F+   E+KS+ +T T D   P      G + W+DGKH V SP+ +S
Sbjct: 725 FKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CsGy1G015000 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 555.4 bits (1430), Expect = 7.0e-158
Identity = 325/780 (41.67%), Postives = 453/780 (58.08%), Query Frame = 0

Query: 12  ILILFSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKA 71
           +L+  S   I   A  A K++I  +   S         +  +  +H+    + F  + + 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGS---------MPSIFPTHYHWYSTEFAEESR- 67

Query: 72  RNAIFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGV 131
              I + Y    +GF+A++  +EA  L  HP V AV  +R ++LHTT S +F+ L+    
Sbjct: 68  ---IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ-- 127

Query: 132 IPPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDRVPC 191
                 W  +  G DVII   DTG+WPE +SF +  + GP+P +W+G C +        C
Sbjct: 128 ---KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFSPRNC 187

Query: 192 NRKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGV 251
           NRK+IGA++F KG  A +       +   S RD DGHG+HT STA G +   AS+ G   
Sbjct: 188 NRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYAS 247

Query: 252 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGE---PADY 311
           G AKG +PKAR+AAYKVCW  +D GC D+DI  AFD A+ D VDV+S+S+GG     + Y
Sbjct: 248 GVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPY 307

Query: 312 YDDGIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQN 371
           Y D IAI ++ A  KGI V  SAGN GP   +V+N APW+ TVGAST+DR F A   L +
Sbjct: 308 YLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGD 367

Query: 372 GHRYMGSSLSKGLK-GDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLR 431
           GHR  G SL  G+    +++P++   ++   +A+     LC   TLD  +V+GKI++C R
Sbjct: 368 GHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVICDR 427

Query: 432 GDTARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKSTK 491
           G + RV KG     AG VGMIL N   +G   + D H++PA  +  N+G  + +Y  S  
Sbjct: 428 GSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHP 487

Query: 492 NPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTG 551
           NP+  +      V  KPAP +A+FS RGPN +SPEI+KPD+ APGVNI+AA+++AV PTG
Sbjct: 488 NPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTG 547

Query: 552 EPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPM 611
            P D R   F  +SGTSM+CPHVSG   LL++ HP WSP+ I+SA+MT+  + DN+ + +
Sbjct: 548 LPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSL 607

Query: 612 LDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDG 671
           +D  +   A  TP+ YGSGH+    A++PGLVYD++ +DY+ FLC+ GY  KTIQ  +  
Sbjct: 608 IDESTGKSA--TPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRT 667

Query: 672 PFKCPAS--ASILNLNYPSIGV---QNLTG--SVTVTRKLKNV-STPGVYKGRVRHPNGV 731
           P +CP +   S  NLNYPSI      N  G  S TV R   NV     VY+ R+  P GV
Sbjct: 668 PVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGV 727

Query: 732 KVLVKPKVLKFERVGEEKSFELTITGD-----VPEDQVVDGVLIWTD-GKHFVRSPIVVS 773
            V VKP  L F    + +S+ +T+T +     + E   V G + W D GKH VRSPIVV+
Sbjct: 728 TVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of CsGy1G015000 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 553.5 bits (1425), Expect = 2.7e-157
Identity = 326/783 (41.63%), Postives = 457/783 (58.37%), Query Frame = 0

Query: 16  FSLLLIISPAMAAKKSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAI 75
           F LL + SP+ +A  S      S ++ + V  E    +  +H     S   S   +  +I
Sbjct: 8   FFLLTLSSPSSSASSS-----NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 67

Query: 76  FYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHL---EKNGVI 135
            ++Y    +GF+A +  ++A+QL  HP V +V+P + + LHTT S EF+ L   +K G++
Sbjct: 68  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 127

Query: 136 PPSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC-TDKTLDRVPCN 195
             S        G D++I  +DTGVWPE  SF + G+ GPVP KWKG C   +      CN
Sbjct: 128 EESD------FGSDLVIGVIDTGVWPERPSFDDRGL-GPVPIKWKGQCIASQDFPESACN 187

Query: 196 RKLIGAKYFNKGFLAYLKSENLTALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVG 255
           RKL+GA++F  G+ A     N T     S RD DGHG+HT S + G YV  AS  G   G
Sbjct: 188 RKLVGARFFCGGYEATNGKMNETT-EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 247

Query: 256 TAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDG 315
            A G +PKAR+AAYKVCW   + GC+D+DI  AFD A+ D VDV+SLS+GG    YY D 
Sbjct: 248 VAAGMAPKARLAAYKVCW---NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDA 307

Query: 316 IAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRY 375
           IAI AF A+ +GI V  SAGN GPGA TV+N APW+ TVGA T+DR+F A V+L NG   
Sbjct: 308 IAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMI 367

Query: 376 MGSSL--SKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDT 435
            G S+    GL   ++YPL+ G      +  +  + LC   +LD + VKGKI++C RG  
Sbjct: 368 SGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS--SSLCLEGSLDPNLVKGKIVLCDRGIN 427

Query: 436 ARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYI------K 495
           +R  KGE     G +GMI+ N    G   +AD HVLPA+ +  + G  +  YI      +
Sbjct: 428 SRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSR 487

Query: 496 STKNPMGYLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVS 555
           S+K+P   ++    ++  +PAP +A+FS+RGPN  +PEI+KPDV APG+NI+AA+ + + 
Sbjct: 488 SSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIG 547

Query: 556 PTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTK 615
           P+G   DNR   F  +SGTSM+CPHVSGL  LL+  HP WSP+AI+SA++T+A   DN+ 
Sbjct: 548 PSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSG 607

Query: 616 KPMLDGGSPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAF 675
           +PM+D  + +   S+   YGSGH+ PT A+DPGLVYD++  DY+ FLC S Y    I   
Sbjct: 608 EPMMDESTGN--TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTI 667

Query: 676 SDGPFKCPA---SASILNLNYPSIGV--QNLTGSVTVTRKLKNVSTPG----VYKGRVRH 735
           +     C     +  + NLNYPS  V  Q    S   T  ++ V+  G    VY+ ++R 
Sbjct: 668 TRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRP 727

Query: 736 PNGVKVLVKPKVLKFERVGEEKSFELTI-TGDVP----EDQVVDGVLIWTDGKHFVRSPI 773
           P G  V V+P+ L F RVG++ SF + + T +V        V  G ++W+DGK  V SP+
Sbjct: 728 PRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 770

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JXC55.2e-26760.62Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
Q9ZSP51.0e-23354.22Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
I1N4623.4e-18948.17Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653513.1e-16647.03Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
O496079.9e-15741.67Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
KAE8652826.10.099.34hypothetical protein Csa_022873 [Cucumis sativus][more]
XP_031739111.10.099.07subtilisin-like protease SBT5.4 [Cucumis sativus][more]
XP_008464322.10.094.72PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo][more]
XP_038895602.10.087.45subtilisin-like protease SBT5.4 [Benincasa hispida][more]
XP_022142562.10.079.08subtilisin-like protease SBT5.4 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A0A0LVY80.099.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1[more]
A0A1S3CLB70.094.72subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=... [more]
A0A6J1CN430.079.08subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P... [more]
A0A0A0LYF10.078.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1[more]
A0A5D3CZ660.079.57Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
AT5G59810.13.7e-26860.62Subtilase family protein [more]
AT2G04160.17.1e-23554.22Subtilisin-like serine endopeptidase family protein [more]
AT5G67360.12.2e-16747.03Subtilase family protein [more]
AT4G34980.17.0e-15841.67subtilisin-like serine protease 2 [more]
AT3G14240.12.7e-15741.63Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 223..236
score: 33.16
coord: 560..576
score: 51.76
coord: 144..163
score: 32.49
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 146..646
e-value: 1.5E-180
score: 602.9
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 115..637
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 678..771
e-value: 6.2E-26
score: 90.5
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 144..602
e-value: 8.9E-43
score: 147.0
NoneNo IPR availableGENE3D3.50.30.30coord: 356..500
e-value: 1.5E-180
score: 602.9
NoneNo IPR availableGENE3D2.60.40.2310coord: 650..774
e-value: 5.4E-36
score: 125.2
NoneNo IPR availablePANTHERPTHR43806:SF40BNAC02G33520D PROTEINcoord: 14..773
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 14..773
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 120..633
score: 28.20417
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 363..492
e-value: 3.38079E-36
score: 130.611
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 414..484
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 30..111
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 31..115
e-value: 4.0E-14
score: 53.1
IPR003137PA domainPFAMPF02225PAcoord: 404..477
e-value: 9.9E-11
score: 41.6
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 22..116
e-value: 2.5E-23
score: 84.3
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 561..571
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 113..596
e-value: 2.4528E-131
score: 390.806

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G015000.2CsGy1G015000.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008236 serine-type peptidase activity