CsGy1G007590 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G007590
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationGy14Chr1: 4875163 .. 4876828 (-)
RNA-Seq ExpressionCsGy1G007590
SyntenyCsGy1G007590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGCTTGGTCGGTTATTTTATTCTTTATTTAAATCACATTCCTCTTCTAAGTCAATTCCCCTCACCATTTAATAATTTAAAATTAAATCAAACAAAAGCACACTCCCTTCTTCATTCCAATTCATTATTATTTTATTTTGAAATATTTGGCTATAAAAAGAGTGTTGTGGTGCCATTATTTTGCAGATCCAATCAAAACCAAACAAATTGTATTAATTGTGAAAACACATTTAATTAGAAGAAGAGATGATGAGAATGGCAACAAATATGAGTGATGAACAACTCCCAGTTGTGAAGTTTTGTTCACAGAATTTGAAGAGTGGAACCGAGGAATGGGTTTCCACAACGAAGAAAGTTCGGGAAGCTCTTGAGGAATATGGTTGTTTCGTAGCACTATACGACACCGTTTCAGCAGAAGTAAGTTCCAATATGTTTGATTCTTTAAAAGAGTTGTTTGATGAAGTTCCTTTGGAGAGGAAGCTTCAGAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAACCCTCTAATGCCCATCCATGAAAGTATGGGAATCGAACACCCCATTCTCCCCACCAATATCAACTCTTTCACTAATCTCATGTGGCCTTCTCGTGGAAACCACTCTTTCCGGTAATTCATTTTGCTTCATTTTCAAACCAAACCTAAACTACCATCTAATAATATATAGTACCAGGATCCTAAATGTTTTTCTTAATTTATGCAGTGAGAATGTGACAGCGTATGCGAATTTGGTATCGGAATTGGATAAAAGGGTGAAAAGCATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAGAAGCCATATGGAATCCACAAAATATCTTATGAGAATGATAAAATACCGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCATTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAACGAAGTAAATGGTTTACAGATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCGACCTCATCTTTCATTGTCATGGCTGGAGATGCTTTCTTTGTAAGTTCCATCTCATTTTATTAATTAATTTCTTCATCATCATCAAATTAAGATTGACATTAAGATCATGAAAGCCAAACTAATAATTAGTGAATGGTTAACTGTTGGCAGGCATGGAGCAATGGCAGGATCTACTCACCACCACACAGAGTGATAATGAGTGGAAACAAAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATTGTTCAAATTCCTAAAGAATTGGTTGATGACAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTTCGTTTCTACAGCACCGAGAGAGGCCAAAATTCTCCCTGTGCCATCAAAGCTTACTGTGGAGTCGGAAATAGCCTCGTTGATACAGATACTCGGAGCCCTCTTCTCTTACATTGATAAATAAATTCATCATATCCACATCAATTTACTATTACGGATCATACCGAATGACTTTTTAAGTACTTAAAATACTGTATTTAAAACAAACACTTAAAAAGTTATCTAAATGAATAGAGAATCTTAGTTTTATAATTTTAGGATGCACCTTGAATTTTGAGGCTATAGTCGTTGGTGGCTTGAAATGGCCCGTTCA

mRNA sequence

ATTTGCTTGGTCGGTTATTTTATTCTTTATTTAAATCACATTCCTCTTCTAAGTCAATTCCCCTCACCATTTAATAATTTAAAATTAAATCAAACAAAAGCACACTCCCTTCTTCATTCCAATTCATTATTATTTTATTTTGAAATATTTGGCTATAAAAAGAGTGTTGTGGTGCCATTATTTTGCAGATCCAATCAAAACCAAACAAATTGTATTAATTGTGAAAACACATTTAATTAGAAGAAGAGATGATGAGAATGGCAACAAATATGAGTGATGAACAACTCCCAGTTGTGAAGTTTTGTTCACAGAATTTGAAGAGTGGAACCGAGGAATGGGTTTCCACAACGAAGAAAGTTCGGGAAGCTCTTGAGGAATATGGTTGTTTCGTAGCACTATACGACACCGTTTCAGCAGAAGTAAGTTCCAATATGTTTGATTCTTTAAAAGAGTTGTTTGATGAAGTTCCTTTGGAGAGGAAGCTTCAGAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAACCCTCTAATGCCCATCCATGAAAGTATGGGAATCGAACACCCCATTCTCCCCACCAATATCAACTCTTTCACTAATCTCATGTGGCCTTCTCGTGGAAACCACTCTTTCCGTGAGAATGTGACAGCGTATGCGAATTTGGTATCGGAATTGGATAAAAGGGTGAAAAGCATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAGAAGCCATATGGAATCCACAAAATATCTTATGAGAATGATAAAATACCGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCATTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAACGAAGTAAATGGTTTACAGATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCGACCTCATCTTTCATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGCAGGATCTACTCACCACCACACAGAGTGATAATGAGTGGAAACAAAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATTGTTCAAATTCCTAAAGAATTGGTTGATGACAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTTCGTTTCTACAGCACCGAGAGAGGCCAAAATTCTCCCTGTGCCATCAAAGCTTACTGTGGAGTCGGAAATAGCCTCGTTGATACAGATACTCGGAGCCCTCTTCTCTTACATTGATAAATAAATTCATCATATCCACATCAATTTACTATTACGGATCATACCGAATGACTTTTTAAGTACTTAAAATACTGTATTTAAAACAAACACTTAAAAAGTTATCTAAATGAATAGAGAATCTTAGTTTTATAATTTTAGGATGCACCTTGAATTTTGAGGCTATAGTCGTTGGTGGCTTGAAATGGCCCGTTCA

Coding sequence (CDS)

ATGATGAGAATGGCAACAAATATGAGTGATGAACAACTCCCAGTTGTGAAGTTTTGTTCACAGAATTTGAAGAGTGGAACCGAGGAATGGGTTTCCACAACGAAGAAAGTTCGGGAAGCTCTTGAGGAATATGGTTGTTTCGTAGCACTATACGACACCGTTTCAGCAGAAGTAAGTTCCAATATGTTTGATTCTTTAAAAGAGTTGTTTGATGAAGTTCCTTTGGAGAGGAAGCTTCAGAATGTTTCTGAGAAGCCATACCATGGCTACTTTGGTCAAAACCCTCTAATGCCCATCCATGAAAGTATGGGAATCGAACACCCCATTCTCCCCACCAATATCAACTCTTTCACTAATCTCATGTGGCCTTCTCGTGGAAACCACTCTTTCCGTGAGAATGTGACAGCGTATGCGAATTTGGTATCGGAATTGGATAAAAGGGTGAAAAGCATGGTGTTTGAAAGCTATGGGGTTGGGAATGCATTAAGAAGCCATATGGAATCCACAAAATATCTTATGAGAATGATAAAATACCGAGTCCCAAAGGAAAAAGAGATGAATTTGGGAGCATTTCCTCATACTGATAAGAGCTTTCTCACAATTCTTCATCAAAACGAAGTAAATGGTTTACAGATCAAAACAAGAGATAACAAATGGATTCAATATCATCCTTTTTCTTCTTCTTCGACCTCATCTTTCATTGTCATGGCTGGAGATGCTTTCTTTGCATGGAGCAATGGCAGGATCTACTCACCACCACACAGAGTGATAATGAGTGGAAACAAAGAGAGATATTCAGTTGGATTGTTTTCATTCAACAATGGAATTGTTCAAATTCCTAAAGAATTGGTTGATGACAAACATCCACAATTGTTTAAACCCTTTGATCATCAAGACTATCTTCGTTTCTACAGCACCGAGAGAGGCCAAAATTCTCCCTGTGCCATCAAAGCTTACTGTGGAGTCGGAAATAGCCTCGTTGATACAGATACTCGGAGCCCTCTTCTCTTACATTGA

Protein sequence

MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH*
Homology
BLAST of CsGy1G007590 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.2e-74
Identity = 138/316 (43.67%), Postives = 205/316 (64.87%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           QLPV+ F  QNLK G+ +W   T  V +ALE+YGCF A +D +S E++ ++F+++++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF- 72

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
           E+P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN S  
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP------KEKE 191
           E +  ++  + ELD  V+ M+ ES+G+ N +  H+ ST YL R++KY  P       ++E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 192 MNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWS 251
             LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALL 252

Query: 252 NGRIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 311
           NGR++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 312 YSTERGQNSPCAIKAY 320
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 319

BLAST of CsGy1G007590 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.6e-74
Identity = 137/315 (43.49%), Postives = 205/315 (65.08%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           QLPV+ F  QNLK G+ +W      V +ALE+YGCF A +D +S E++ ++F+++++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF- 72

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
           E+P+  K +NVS KP+HGY   N    ++ES+GI+   +   +N FT  +WP  GN S  
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP-----KEKEM 191
           E +  ++  + ELD  V+ M+ ES+G+ N +  H+ ST YL R++KY  P      ++E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 192 NLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSN 251
            LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  N
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALLN 252

Query: 252 GRIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRFY 311
           GR++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F+
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 312

Query: 312 STERGQNSPCAIKAY 320
            TE G+ +  A+ A+
Sbjct: 313 QTEAGRIAQSALHAF 318

BLAST of CsGy1G007590 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.3e-72
Identity = 134/305 (43.93%), Postives = 198/305 (64.92%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           QLPV+ F  QNLK G+ +W   T  V +ALE+YGCF A +D +S E++ ++F+++++LF 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF- 65

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
           E+P+  K +NVS K +HGY   N    ++ES+GI    +   +N FT  +WP  GN S  
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP----KEKEMN 191
           E +  ++  + ELD  V+ M+ ES+G+   +  H+ ST YL R++KY  P     ++E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 192 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 251
           LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  NG
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALLNG 245

Query: 252 RIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYS 311
           R++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F+ 
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 301

BLAST of CsGy1G007590 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-60
Identity = 124/308 (40.26%), Postives = 180/308 (58.44%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           +LPV+    +NLK GT  W S   +VREALEEYGCF A+ D VS E+   + +   EL +
Sbjct: 8   RLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLN 67

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
            +PLE K+ N ++  Y G+     +  ++E MG+        +  FTNLMWP  GN  F 
Sbjct: 68  -LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFC 127

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 191
           E V +Y   ++E+D  +K MVFES+G+     S  EST YL+  ++Y+ P +   +    
Sbjct: 128 ETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALS 187

Query: 192 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 251
            H DKS LTI++QN+V GL+ +T+D +WI        +  + IV+ GD F AWSNGR++S
Sbjct: 188 LHKDKSILTIVNQNDVKGLEFETKDGEWI------LPTADNHIVLLGDCFMAWSNGRLHS 247

Query: 252 PPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQN 311
           P HRV +  N+ R S   FSF   I++ P ELVD++HP LF PF+  + L +  T+ G  
Sbjct: 248 PLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAK 304

Query: 312 SPCAIKAY 320
           + C +K Y
Sbjct: 308 AVCDLKQY 304

BLAST of CsGy1G007590 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.5e-53
Identity = 129/434 (29.72%), Postives = 200/434 (46.08%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           QLP++    + L+ G+ +W      VR+ALE++GCF A YD VS E+  ++  +++ELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELF- 66

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP-SRGNHSF 131
            +P+E K +NV  KPY GY   N L    ES+GI +  +  N+N FT  +WP   GN + 
Sbjct: 67  ALPVETKQRNVCPKPYVGYLNHNNL---SESLGISNANILENVNEFTQQLWPDGDGNENI 126

Query: 132 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE------- 191
            + +  +A  + E+D  V+ MV ES+G+   +  H++ST+Y MR++KY  P E       
Sbjct: 127 SKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 192 ------------------------------------------------------------ 251
                                                                       
Sbjct: 187 DDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAE 246

Query: 252 -------------------------------------------------KEMNLGAFPHT 311
                                                            +E  LG   HT
Sbjct: 247 ANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHT 306

Query: 312 DKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYSPPH 326
           DK+  T+L Q+E+ GL++KT+D KWI+  P    S ++FIV+AGD+  A  NGRI +P H
Sbjct: 307 DKNLFTVLFQHEIEGLEVKTKDEKWIRVKP----SPNTFIVIAGDSLCALMNGRIRAPYH 366

BLAST of CsGy1G007590 vs. NCBI nr
Match: XP_031738134.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571.1 hypothetical protein Csa_014276 [Cucumis sativus])

HSP 1 Score: 697 bits (1799), Expect = 1.66e-253
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS 60
           MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS
Sbjct: 1   MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS 60

Query: 61  NMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNL 120
           NMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNL
Sbjct: 61  NMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNL 120

Query: 121 MWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRV 180
           MWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRV
Sbjct: 121 MWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRV 180

Query: 181 PKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDA 240
           PKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDA
Sbjct: 181 PKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDA 240

Query: 241 FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY 300
           FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY
Sbjct: 241 FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY 300

Query: 301 LRFYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           LRFYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH
Sbjct: 301 LRFYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338

BLAST of CsGy1G007590 vs. NCBI nr
Match: XP_004151475.3 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570.1 hypothetical protein Csa_013560 [Cucumis sativus])

HSP 1 Score: 697 bits (1798), Expect = 2.36e-253
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS 60
           MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS
Sbjct: 1   MMRMATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS 60

Query: 61  NMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNL 120
           NMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNL
Sbjct: 61  NMFDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNL 120

Query: 121 MWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRV 180
           MWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRV
Sbjct: 121 MWPSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRV 180

Query: 181 PKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDA 240
           PKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDA
Sbjct: 181 PKEKEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDA 240

Query: 241 FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY 300
           FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY
Sbjct: 241 FFAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDY 300

Query: 301 LRFYSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           LRFYSTERGQNSPCAIKAYCGVGNSL+DTDTRSPLLLH
Sbjct: 301 LRFYSTERGQNSPCAIKAYCGVGNSLIDTDTRSPLLLH 338

BLAST of CsGy1G007590 vs. NCBI nr
Match: TYJ99087.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa])

HSP 1 Score: 652 bits (1681), Expect = 1.40e-235
Identity = 314/335 (93.73%), Postives = 326/335 (97.31%), Query Frame = 0

Query: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63
           MATNMSDE LPVVKFCS+NLKSGT+EWV T KKVREALE+YGCFVALYD+VSAEVSSNMF
Sbjct: 1   MATNMSDE-LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMF 60

Query: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123
           DSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 61  DSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120

Query: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183
           SRGN SFRE+VT YA LVSELD+RVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPKE
Sbjct: 121 SRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE 180

Query: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA
Sbjct: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 240

Query: 244 WSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 303
           WSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIP+ELVDDKHPQLF PFDHQD+LRF
Sbjct: 241 WSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRF 300

Query: 304 YSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           YSTERGQNSPCAIKAYCGVGNSL+D DTRSPLLLH
Sbjct: 301 YSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

BLAST of CsGy1G007590 vs. NCBI nr
Match: XP_038893580.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 607 bits (1566), Expect = 4.04e-218
Identity = 291/331 (87.92%), Postives = 310/331 (93.66%), Query Frame = 0

Query: 8   MSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLK 67
           M D Q+P+VKFCS+NLKSG +EWV T +KVREALEEYGCFVALYDTVS +VSSNMF SLK
Sbjct: 1   MFDFQIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLK 60

Query: 68  ELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 127
           +LFD VPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPS GN
Sbjct: 61  QLFD-VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGN 120

Query: 128 HSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMN 187
             F ENVT YA LVSELD+RVKSMVFESYGVG  L++HMESTKYLMRMIKYRVPKEKEMN
Sbjct: 121 RPFCENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMN 180

Query: 188 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNG 247
           LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNG
Sbjct: 181 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNG 240

Query: 248 RIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTE 307
           RIYSPPHRV++S NKERYSVGLFSFN+GI+QIPKELVDDKHPQ F PFDHQDYLRFY+TE
Sbjct: 241 RIYSPPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTE 300

Query: 308 RGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           RGQNSPCAIKAYCGVGNSLVDTD+RS LLLH
Sbjct: 301 RGQNSPCAIKAYCGVGNSLVDTDSRSTLLLH 330

BLAST of CsGy1G007590 vs. NCBI nr
Match: XP_038895877.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 604 bits (1557), Expect = 9.16e-217
Identity = 288/327 (88.07%), Postives = 308/327 (94.19%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           ++P+VKFCS+NLKSG +EWV T +KVREALEEYGCFVALYDTVS +VSSNMF SLK+LFD
Sbjct: 4   EIPIVKFCSENLKSGRKEWVLTREKVREALEEYGCFVALYDTVSMDVSSNMFHSLKQLFD 63

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
            VPLERK+QNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNI+SFTNLMWPS GN  F 
Sbjct: 64  -VPLERKIQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNIHSFTNLMWPSHGNRPFC 123

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 191
           ENVT YA LVSELD+RVKSMVFESYGVG  L++HMESTKYLMRMIKYRVPKEKEMNLGAF
Sbjct: 124 ENVTLYAKLVSELDERVKSMVFESYGVGKVLKTHMESTKYLMRMIKYRVPKEKEMNLGAF 183

Query: 192 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 251
           PHTDKSFLTILHQNEVNGLQIKTRDNKW+QYHP SSSSTSSFIVMAGDAFFAWSNGRIYS
Sbjct: 184 PHTDKSFLTILHQNEVNGLQIKTRDNKWLQYHPPSSSSTSSFIVMAGDAFFAWSNGRIYS 243

Query: 252 PPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQN 311
           PPHRV++S NKERYSVGLFSFN+GI+QIPKELVDDKHPQ F PFDHQDYLRFY+TERGQN
Sbjct: 244 PPHRVLVSANKERYSVGLFSFNDGIIQIPKELVDDKHPQQFNPFDHQDYLRFYTTERGQN 303

Query: 312 SPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           SPCAIKAYCGVGNSLVDTD+RS LLLH
Sbjct: 304 SPCAIKAYCGVGNSLVDTDSRSTLLLH 329

BLAST of CsGy1G007590 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 652 bits (1681), Expect = 6.76e-236
Identity = 314/335 (93.73%), Postives = 326/335 (97.31%), Query Frame = 0

Query: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63
           MATNMSDE LPVVKFCS+NLKSGT+EWV T KKVREALE+YGCFVALYD+VSAEVSSNMF
Sbjct: 1   MATNMSDE-LPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSSNMF 60

Query: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123
           DSLKELFD+VPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 61  DSLKELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120

Query: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183
           SRGN SFRE+VT YA LVSELD+RVK MVFESYGVGNAL+SHMESTKYLMRMIKYRVPKE
Sbjct: 121 SRGNDSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKE 180

Query: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA
Sbjct: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 240

Query: 244 WSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 303
           WSNGRIYSPPHRVIMSGN+ERYSVGLFSFNNGIVQIP+ELVDDKHPQLF PFDHQD+LRF
Sbjct: 241 WSNGRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRF 300

Query: 304 YSTERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           YSTERGQNSPCAIKAYCGVGNSL+D DTRSPLLLH
Sbjct: 301 YSTERGQNSPCAIKAYCGVGNSLIDMDTRSPLLLH 334

BLAST of CsGy1G007590 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 538 bits (1386), Expect = 4.29e-191
Identity = 264/332 (79.52%), Postives = 292/332 (87.95%), Query Frame = 0

Query: 8   MSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLK 67
           M++ QLP+VKF  +NL+S    WVST +KVREALEEYGCFVALYD+VS E+SSNMFDSLK
Sbjct: 1   MAEFQLPLVKFSEENLRS----WVSTREKVREALEEYGCFVALYDSVSMELSSNMFDSLK 60

Query: 68  ELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 127
           ELF EVPLERKL+NVSEKPYHGYFGQNPLMPIHESMGIE P L +   SFTNLMWPS GN
Sbjct: 61  ELF-EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGN 120

Query: 128 HSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMN 187
            SF ENV +YA  ++ELD+ VK MVFESYGV   L SHM+ST+YL+RMIKYRVPK+ EMN
Sbjct: 121 KSFCENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMN 180

Query: 188 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSS-TSSFIVMAGDAFFAWSN 247
           +GAFPHTDKSFLTILHQN+VNGLQIKT+ NKW+QYHP SSSS TSSFIVMAGDAFFAWSN
Sbjct: 181 MGAFPHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSN 240

Query: 248 GRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYST 307
           GRIYSPPH+VIMSG +ERYSVGLFSFN+GIVQIPKELVD+ HPQ FKPFDHQDYLRFYST
Sbjct: 241 GRIYSPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYST 300

Query: 308 ERGQNSPCAIKAYCGVGNSLVDTDTRSPLLLH 338
           ERGQNSPCAIKAYCG+GN LVDT  R  +LLH
Sbjct: 301 ERGQNSPCAIKAYCGIGNGLVDT--RGCILLH 325

BLAST of CsGy1G007590 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 5.86e-176
Identity = 243/326 (74.54%), Postives = 279/326 (85.58%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 71
           ++PVV+F S NL+SG  +W ST +KVREALEEYGCFVALYD+VS E SS  M +++KE+F
Sbjct: 4   EIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMKEVF 63

Query: 72  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 131
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123

Query: 132 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 191
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183

Query: 192 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 251
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSNGRI
Sbjct: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNGRI 243

Query: 252 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 311
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303

Query: 312 QNSPCAIKAYCGVGNSLVDTDTRSPL 335
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 328

BLAST of CsGy1G007590 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 3.93e-174
Identity = 241/326 (73.93%), Postives = 278/326 (85.28%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSS-NMFDSLKELF 71
           +LPVV+F S NL+SG  +W ST +KVREALEEYGCFVAL D+VS E SS  M ++++E+F
Sbjct: 4   ELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMEEVF 63

Query: 72  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 131
            EVPLERK+QNVSEKP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N  F
Sbjct: 64  -EVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123

Query: 132 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 191
            EN+ +YA LVS+L++ VK MVFESYG+ +   SH +STKYL+RMIKYRVP   EMNLGA
Sbjct: 124 CENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183

Query: 192 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSNGRI 251
           FPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFI+MAGDAFFAWSNGRI
Sbjct: 184 FPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNGRI 243

Query: 252 YSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 311
           YS PHRV MSGN+ERYSVGLFSFN+GIVQIPKELVDDKHPQ FK FDHQDYLRFYSTE+G
Sbjct: 244 YSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 303

Query: 312 QNSPCAIKAYCGVGNSLVDTDTRSPL 335
           Q+SP AIKAYCGV N L+D+ T  P+
Sbjct: 304 QSSPSAIKAYCGVANGLLDSRTCIPM 328

BLAST of CsGy1G007590 vs. ExPASy TrEMBL
Match: A0A0A0LT96 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044830 PE=4 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 1.50e-172
Identity = 240/242 (99.17%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 4   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 63
           MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF
Sbjct: 1   MATNMSDEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMF 60

Query: 64  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 123
           DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP
Sbjct: 61  DSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWP 120

Query: 124 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 183
           SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE
Sbjct: 121 SRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKE 180

Query: 184 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFA 243
           KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFF 
Sbjct: 181 KEMNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFV 240

Query: 244 WS 245
            S
Sbjct: 241 SS 242

BLAST of CsGy1G007590 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 3.8e-84
Identity = 149/311 (47.91%), Postives = 212/311 (68.17%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVA-LYDTVSAEVSSNMFDSLKELF 71
           ++P + F  ++LK GT+ W ST + +R+ALEEYGCF+  L D    ++   +F SL +LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 72  DEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSF 131
           D +P + K++N  +KP +GY GQ P +P+HES+GI++        SFT LMWP +GN  F
Sbjct: 64  D-LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHF 123

Query: 132 RENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGA 191
            E +  YA   +ELD+ V  MVF+SY V      ++EST YL+R++K R P  +   LG 
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 192 FPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIY 251
             HTDKSF TILHQ++VNGL+++TR+ + I     + SS S F+V+AGDA  AWSN R++
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERIN---INLSSPSLFMVVAGDALMAWSNDRVW 243

Query: 252 SPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERGQ 311
           SP H+V++SG  +RYS+G+F+FNNG +Q+P+EL+D +HP ++KPFDH   LRFY T+ G 
Sbjct: 244 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 303

Query: 312 NSPCAIKAYCG 322
            S C IKAYCG
Sbjct: 304 KSECPIKAYCG 309

BLAST of CsGy1G007590 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 308.1 bits (788), Expect = 8.5e-84
Identity = 144/313 (46.01%), Postives = 218/313 (69.65%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           +LPV+ F ++NLK G  EW  T   V++AL++YG F A +D +  E+  ++F +L+ELFD
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
            +PL+ KL+NVS+KP+HGY GQ P++P++ESMGI+   +   +++FT  +WP +GN SF 
Sbjct: 71  -LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFS 130

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPKEKEMNLGAF 191
             + +++  +SELD  ++ M+ ES+G+   +  H+ ST YL+R++KY+ P  +E  +G  
Sbjct: 131 TTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLN 190

Query: 192 PHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSNGRIYS 251
            HTDK+ +TIL+QN V GL+++T+D  WI+  P    +  SF VM GD+ +A  NGR++S
Sbjct: 191 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP----TQDSFTVMIGDSLYALLNGRLHS 250

Query: 252 PPHRVIMSGNKERYSVGLFSFNNG--IVQIPKELVDDKHPQLFKPFDHQDYLRFYSTERG 311
           P HRV+M+G + RYS+GLFS      IV  P ELVD++HP+LFKPFDH ++L+FY TE G
Sbjct: 251 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 310

Query: 312 QNSPCAIKAYCGV 323
           Q S  A+K YCG+
Sbjct: 311 QRSQSALKTYCGI 317

BLAST of CsGy1G007590 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 7.2e-83
Identity = 149/321 (46.42%), Postives = 217/321 (67.60%), Query Frame = 0

Query: 4   MATNMSDEQLPVVKFCS-QNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNM 63
           MA+ +   ++P++   S Q LK  T  W S +++  EALEEYGCF+A+YD V+ ++  ++
Sbjct: 1   MASILKTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSI 60

Query: 64  FDSLKELFDEVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMW 123
           F + +ELFD +P E K +NV+EKPYHGY GQ P++P+HE +G+++         FT+LMW
Sbjct: 61  FAAAEELFD-LPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMW 120

Query: 124 PSRGNHSFRENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVPK 183
           P +GN  F   V  ++N V+ELD+ V  M+FE+YGV     SH+ S  YL++ +KY  P 
Sbjct: 121 P-QGNDRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPP 180

Query: 184 EKEMNLGAFP-HTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAF 243
           E  +++ AFP HTDK+FL+ILHQN+VNGL++K++D +WI           S++VMAGD  
Sbjct: 181 E-SISMPAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISL----QLPPKSYVVMAGDIS 240

Query: 244 FAWSNGRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYL 303
             WSN RI S  HRV M G+K RY++GLFSF   +V IP+ELVDDKHP ++KPFD+   +
Sbjct: 241 MGWSNDRIRSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALI 300

Query: 304 RFYSTERGQNSPCAIKAYCGV 323
            FY+T+ G+ +   +KAYCG+
Sbjct: 301 NFYTTKEGREANSTLKAYCGI 314

BLAST of CsGy1G007590 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 8.5e-76
Identity = 138/316 (43.67%), Postives = 205/316 (64.87%), Query Frame = 0

Query: 12  QLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKELFD 71
           QLPV+ F  QNLK G+ +W   T  V +ALE+YGCF A +D +S E++ ++F+++++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF- 72

Query: 72  EVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNHSFR 131
           E+P+  K +NVS KP+HGY   N    ++ES+GI    +   +N FT  +WP  GN S  
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 132 ENVTAYANLVSELDKRVKSMVFESYGVGNALRSHMESTKYLMRMIKYRVP------KEKE 191
           E +  ++  + ELD  V+ M+ ES+G+ N +  H+ ST YL R++KY  P       ++E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 192 MNLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWS 251
             LG   HTDK+ +TILHQ +V+GL++KT+D+KWI+  P    S  S +VM GD+  A  
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP----SQDSVLVMVGDSLCALL 252

Query: 252 NGRIYSPPHRVIMSGNKERYSVGLFSF--NNGIVQIPKELVDDKHPQLFKPFDHQDYLRF 311
           NGR++SP HRVIM+G K RYS GLFS      I+  P+ELVD +HP++FKPF++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 312 YSTERGQNSPCAIKAY 320
           + TE G+ +  A+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 319

BLAST of CsGy1G007590 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 3.0e-65
Identity = 128/319 (40.13%), Postives = 194/319 (60.82%), Query Frame = 0

Query: 10  DEQLPVVKFCSQNLKSGTEEWVSTTKKVREALEEYGCFVALYDTVSAEVSSNMFDSLKEL 69
           ++ LP++ F S  L  GT  W++T   VR A+E  G FVA +  VS+++  N+   +KE+
Sbjct: 6   EQCLPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEM 65

Query: 70  FDEVPLERKLQNVSEKPYHGYFGQ-NPLMPIHESMGIEHPILPTNINSFTNLMWPSRGNH 129
           +  +P + K++N + K  HGY         IHES+GI++         F+ L+WP +GN 
Sbjct: 66  Y-YLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGND 125

Query: 130 SFRENVTAYANLVSELDKRVKSMVFESYGVGNALR--SHMESTKYLMRMIKYRVPKEKEM 189
            F +    YA  ++ELD+ V  M++ESYG+       SH EST+YL+RM+ YR  +  E 
Sbjct: 126 PFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEA 185

Query: 190 NLGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSSSSTSSFIVMAGDAFFAWSN 249
           N G   HTDKSF++ILHQN V GLQ+KT   +W+ ++P    S + F+V++G    AWSN
Sbjct: 186 NTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP----SPTRFVVLSGMGLTAWSN 245

Query: 250 GRIYSPPHRVIMSGNKERYSVGLFSFNNGIVQIPKELVDDKHPQLFKPFDHQDYLRFYST 309
            RI +  H+V+MS ++ RYS+G FSF+ G ++ P+ELVDD+HP  + PF+H   LRFY +
Sbjct: 246 DRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYES 305

Query: 310 ---ERGQNSPCAIKAYCGV 323
                 ++S   ++ YCGV
Sbjct: 306 YINSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZTA31.2e-7443.67Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q945B61.6e-7443.49Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q944Z57.3e-7243.93Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
A0A0N9HQ361.7e-6040.26Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=... [more]
Q945B51.5e-5329.722-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
XP_031738134.11.66e-253100.00probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652571... [more]
XP_004151475.32.36e-25399.70probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8652570... [more]
TYJ99087.11.40e-23593.73putative 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo var. makuwa][more]
XP_038893580.14.04e-21887.92probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
XP_038895877.19.16e-21788.07probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A5D3BIZ76.76e-23693.73Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O... [more]
A0A6J1C7124.29e-19179.52probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara... [more]
A0A6J1EAT75.86e-17674.54probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1E7C83.93e-17473.93probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
A0A0A0LT961.50e-17299.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G044830 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52790.13.8e-8447.912-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.18.5e-8446.012-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52800.17.2e-8346.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.18.5e-7643.672-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G80320.13.0e-6540.132-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 14..92
e-value: 4.5E-8
score: 33.9
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..303
e-value: 1.7E-56
score: 194.1
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 178..272
e-value: 3.1E-18
score: 66.1
NoneNo IPR availablePANTHERPTHR47990:SF172-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKEcoord: 6..321
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 6..321
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..309
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 169..275
score: 10.992504

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G007590.2CsGy1G007590.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding