CsGy1G003060 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy1G003060
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	protein MIS12 homolog
Location	Gy14Chr1: 1916565 .. 1919466 (+)
RNA-Seq Expression	CsGy1G003060
Synteny	CsGy1G003060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTAAGTCCGCTCCATGTCTCCATCAGTAGCCATTACCATTTTGGCGGCAAAATTCCTCCATTTATGCCCAAGCTTTGGAGCTTCCCATTTGAATTTCTTCTCGTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTGGGTAAGAAGGTTTTTCAGGACGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCGTATTGTTCTCTCTGTTTTCTATTTCTTTAATTTAGGTTTTTCAATTTTGTTCGGTTAGGTTAATTTTGTTGATTCTTGTTAATTCTTTTTCCCTTCAGACAAGCGTCGGCTGCCTTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGTAAGGTTTAATTTGTATTATGTTATTTAGCATTTTCTTGTTAATTTCTGAATTCTTAAGTTTGTACTCTTAAGCACTTCTTTGAATTGTTCACATTTTCGCCTCGTTTTATTCAGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAGATGTAGGTTCTTCTTTCTTAATGAGGCTCCTTGGCCAAAGAATAAAAATATCATGTTGGTATCAATATCTGAATTTTAGTTTGTAGTTTATCGATTATATATCAACCTTGATAAATATTTATGTAAAGCAATAAATTAACCGAAATTAGCTATTGAATTGCTTTTGCTTTCAAACTAAGTTGTAGATCTTGCTTTTAAGTAATATTCATATTGAGATTAATAGATGACATCTTTTTATGTTTTATGTGTTTATAAAGGATAGTTGAGATGTTTATAAACCCTTAATATTGATGTCAAATATTCATATATATATATATAACTTTGCCGGTTTCAAAAGTTCTCTATAAATATAACTTACATGTAAATGACCGGTTGCTTATGCAGCGAGTTTCCCATTTATTTTGTACTGACTTGATAGCTTCCTCAATTTTTGGTTCTGGGACGAACAAATTTTGAGGAGAAAGCGCAGTTACTTACTGTGTATCATTGAGTTTCAATTTTTGTTCCAAATATATTCATTGCTACTTTCAGATATAATCACAAGTTTCCATATTTTCAGACCTCAGAGCTTCCTCATTTTGTTAGCAATAACTCAACGTGGTTCCATTGTAACTAGAATGTGGTCGTGACGGTAAAAGATTCTTTTTAGCTCTAAAAGGAATGCGACCAAATGCCTGCAGCACTTATAAAAATCTGTCACTGGGGCATCATGCCTTTTCTTTTTCAATTACATTTGAAATCTCCTATGTAAGATCCAGTTCGATTCCTTTCATTTTGCACCAATATAGTTTACTTTGGGTTGGGCAACTTCTGTCTGTTTCACTGGCATCTCATTGGAATCCCCAATATCTTATTGTCAGGATGCTAAATTATCAATGGTTCCCATCTTGTTTGAGAACTTTGAGTTATGGCTAATTGAGTCTATGTGTTCTTTTAATCGAGTCTGTGTTCTTTTACTACTTTTTCTTCTTTCTAATGCAATAGCTAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTATTGAATATGTCGTATGTTTAACACGTGCGTTTAAGTAATTCATATCTCATGAGGAAATATATTCTTCAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAACTAAGTTGTGCTTAAAACCAATTTTTTCTTCCTGCACTCCTGGGTTTACATGCACACTACTCTACTAGTACACTCGTGAAGGCTTCCTCCGAATGAAATATACAAATAATGGCCTCCTCCGGCGGATCATCCTGGTTTGCATTTCTCATGGTGACACACTCAAATAATTCCAAATGACACTTAAGTTAACATCTGTAAAATCATTTCATTTAGGCAATTTTTATATTACACTAAACTCTAACAGTCTCATTTTCTGTTCATTCCATAAAAGAGGAGCTTCTTGCAGTATGTGAATAAGGCAACTTCTCTTGCTATTTTCATTTTGAATGCCACTTGAACAAAGTTTATTCTTTAGTTATTAAATTTCGTTGTACTCAAACTTCAACAGTCGGTAGGTAAAAGTTGTTCATAATAGTAGGAAGCAGAATTCTAACATGTATCAATTTCTTGCAGAAATGATCAGAACTGCATCGGAGCTTCGTGTGAAAATAGGAAAGCTGAAGAAAAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAAGGGTAGGATTTTCAAGCTACTTTATTATCAATGAAGCTGGTTTTAGCTTTTTTGAAGCTGAATATCATCTCACATGCTCGTTGATGCTTGTTTCTGTTGTGCAGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGAAGTTATTTCACCATGACTTCAATCTTACTGTATATCATGCTTGCTTACGAAACTTGTCACTATGAGTTCTTCAATGTTTAAACTTTATTTAGCAGTTGTGAGATATTCTAAACAAAATTTG

mRNA sequence

GGTTAAGTCCGCTCCATGTCTCCATCAGTAGCCATTACCATTTTGGCGGCAAAATTCCTCCATTTATGCCCAAGCTTTGGAGCTTCCCATTTGAATTTCTTCTCGTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATTGGGTAAGAAGGTTTTTCAGGACGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCACAAGCGTCGGCTGCCTTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAGATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAGAACTGCATCGGAGCTTCGTGTGAAAATAGGAAAGCTGAAGAAAAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGAAGTTATTTCACCATGACTTCAATCTTACTGTATATCATGCTTGCTTACGAAACTTGTCACTATGAGTTCTTCAATGTTTAAACTTTATTTAGCAGTTGTGAGATATTCTAAACAAAATTTG

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCACAAGCGTCGGCTGCCTTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAGATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAGAACTGCATCGGAGCTTCGTGTGAAAATAGGAAAGCTGAAGAAAAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

Protein sequence

MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI*

Homology

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BLAST of CsGy1G003060 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 8.0e-49
Identity = 113/247 (45.75%), Postives = 171/247 (69.23%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDL 60
           MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD--HDVD 120
           + GI++VR L+   +  RL +WE Y L  CF+VP+GF LP  +ES   +S+  D  +D++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 180
           LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EMIRTASELRVKIGKLK-KRTEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEF 239
           EM + ASELR  + +LK +R + ++ A V++      + +H K FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

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BLAST of CsGy1G003060 vs. NCBI nr
Match: XP_004138204.3 (protein MIS12 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 458 bits (1179), Expect = 2.36e-162
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238

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BLAST of CsGy1G003060 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 430 bits (1106), Expect = 3.28e-151
Identity = 226/239 (94.56%), Postives = 230/239 (96.23%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CsGy1G003060 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 428 bits (1100), Expect = 2.69e-150
Identity = 225/239 (94.14%), Postives = 229/239 (95.82%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

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BLAST of CsGy1G003060 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 393 bits (1010), Expect = 4.05e-136
Identity = 206/239 (86.19%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTE SDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+D+SPGVTSISHD+DV LDTE
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210

Query: 181 TASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELR KIGKLKKR  EE+KL  VEKVHTNGDISH+H G SNAKLDDI+ FL DLK +
Sbjct: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 269

[Close]

BLAST of CsGy1G003060 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388 bits (996), Expect = 2.03e-134
Identity = 203/241 (84.23%), Postives = 221/241 (91.70%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD--HDVDLD 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+DESP VTS+S D  +DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180
           TELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 IRTASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKK 238
           + TASELR KIGKLKKR  EE+KL  VEK+HTNGDISHHHKGFSNAKLDDIQEFL+DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKT 240

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BLAST of CsGy1G003060 vs. ExPASy TrEMBL
Match: A0A0A0LPL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1)

HSP 1 Score: 456 bits (1172), Expect = 1.33e-161
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLS LKKI
Sbjct: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSGLKKI 238

[Close]

BLAST of CsGy1G003060 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 1.59e-151
Identity = 226/239 (94.56%), Postives = 230/239 (96.23%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CsGy1G003060 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 428 bits (1100), Expect = 1.30e-150
Identity = 225/239 (94.14%), Postives = 229/239 (95.82%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180
           LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 TASELRVKIGKLKKR-TEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238
           TASELR KIGK+KKR TEE KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CsGy1G003060 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 382 bits (981), Expect = 1.89e-132
Identity = 201/241 (83.40%), Postives = 218/241 (90.46%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD--HDVDLD 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+DESP VTS+S D  +DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180
           TELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MFQEM
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 IRTASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKK 238
           + TASELR KIGKLKKR  EE+KL  VEK+HTNGD SHHHKGFSNAKLDDIQEFL+DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

[Close]

BLAST of CsGy1G003060 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 380 bits (976), Expect = 1.09e-131
Identity = 201/241 (83.40%), Postives = 217/241 (90.04%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD--HDVDLD 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+DESP VTS+S D  +DVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180
           TELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 IRTASELRVKIGKLKKRT-EETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKK 238
           + TASELR KIGKLKKR  EE+KL  VEK+HTN DISHHHKGFSNAKLDDIQEFL+DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLKT 240

[Close]

BLAST of CsGy1G003060 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 195.3 bits (495), Expect = 5.7e-50
Identity = 113/247 (45.75%), Postives = 171/247 (69.23%), Query Frame = 0


Query: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDL 60
           MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHD--HDVD 120
           + GI++VR L+   +  RL +WE Y L  CF+VP+GF LP  +ES   +S+  D  +D++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ 180
           LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EMIRTASELRVKIGKLK-KRTEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEF 239
           EM + ASELR  + +LK +R + ++ A V++      + +H K FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

The following BLAST results are available for this feature:

BLAST of CsGy1G003060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	8.0e-49	45.75	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of CsGy1G003060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138204.3	2.36e-162	100.00	protein MIS12 homolog isoform X1 [Cucumis sativus]	[more]
KAA0058004.1	3.28e-151	94.56	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
KAA0058032.1	2.69e-150	94.14	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
XP_038879945.1	4.05e-136	86.19	protein MIS12 homolog [Benincasa hispida]	[more]
XP_023515499.1	2.03e-134	84.23	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy1G003060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPL2	1.33e-161	99.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1	[more]
A0A5A7US57	1.59e-151	94.56	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	1.30e-150	94.14	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A6J1E0A1	1.89e-132	83.40	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	1.09e-131	83.40	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of CsGy1G003060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	5.7e-50	45.75	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 121..148
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 10..139 e-value: 2.3E-19 score: 69.9
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..237

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy1G003060.2	CsGy1G003060.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus