CsGy1G002820 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy1G002820
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Flavonoid 3' hydroxylase
Location	Gy14Chr1: 1758257 .. 1761503 (+)
RNA-Seq Expression	CsGy1G002820
Synteny	CsGy1G002820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCATGGAGGAAATTACCCCAATCGTCGCCGCCCTTATAACACTCGCCTCCGCCTTCCTCCTCCACCGTGCTTTCTTCCTCCGCCGTAGACTCCGCCTCCCGCCTGGTCCGAAACCATGGCCGATAGTAGGAAACATGCCGCAGATCGGCGAAGCGCATCTCCACTCTCTCCTTGCCACCATGGCCGGAAAGTACGGACCACTGATGTACCTCCGCTTAGGCTCCGTGGACATGGTCGTAGCGGCATCCGCGGCTGTGGCAGAGCAAGTTTTGAAGATTCACGACACTAATTTCCTTAGCCGGCCACCAAACGCCGGCGCAAAATATATTGCTTATAATTATCAGGATATGGTTTTTGCACCGTACGGTCCACGGTGGCGATTGCTCCGGAAGATCAGCACCGTCCATCTCTTCTCATCAAAAGCCCTAGATGATCATCGCCGCATACGGGAGGTTCGTCTTTATATATATATATATATATATTCTTTCTATTTATATGCATGGTATATCCCTGACTCTTTCTTCTCAGAAAACTAGGGTTTCGATTTTGGAAAATATCAAGAAGTTAGATAGTGGTTGGAGAGTTGATGTTAATCTCAATCAAACTTACCTCGACAGTAATTAACATATCTTTTTAATCCGTAAATCTGAAAGAAAAATACAACGATCGTATATATAAATTAAGCTAAATTAAGGGAGAAGTATTTGACCTAATGAGTGAAGTTCATGTAAATTTGTGCTTGAAGGAGACGATAGACCAATACTGGCTGGAGTTTAGGATGTGTACTTGTAAGAGATCGGATGTTTGATCATTATTTGACCACAACAAATCACTATTATTCAAACTAAACAACTATTAACCACACAAAATGAGTTTAAAATTCTATATTTGGTTGTAGAATTTTTGTTTAATTTGTACTTTGTAGTTTTGATATATATGTTTGTGGCCAAACATCTATTGGATATTTTCCATTATTAGTTAAAATAGTACGAAACAAATATGATGAATATATATATTTTCCGATGACTTATAGGTAAAGACTAAACCAAACTCTCAAATAAAATGTGAAACCATTTTTGTGAGGAAAAAAAATGGAAAATTAATCCTGTTTGTGTTACATTAAGATTTTTCATATATTTTATATTGATTTACATAATCAACGTAATTAAATATAAATATGTGATTTAAATAATATAACTTAAAAATTAAGATTTCTTTTTATAATATGTAGTGATGAAGGTAATATTTATTTAATATATTGGGTCATCGTTCAAGTACAGGAAGAGATATCGGTGCTAGTACAGGCCCTTGCAAGGTCCGGTGAAGCTCCAGCGAACTTGGGGAGCTTGCTCACCGTGTGCATTGCGAACTCCCTTGGACGGACAATGATTGGCCGACGTGTTTTCGGTGATGGAAGCGGTAGCGATGACTTAGAGTCAAATCAATTTAAGTTAATGGTGGAGCAAGTGATGGTGCTGGCAGGAAAGTTCAACCCTGGTGACTTTTTCCCCTGGCTTGAGTGGCTTGATTTGATGGGTGTGGGCAGAGAGATGAAGAAGGTCCATAAATGGTTCGATGATTTTTTGACTAAGATCGTGGAGGAGCATCGGAATTTGTTGGCCAGAGGTGTCGGTGGTGGTCACCAAGATCTGTTGAGTACTCTTCTGTCAATGAAGGATGACGGTGAAGATGAGAATGAGAAGCTCAATGATACTGAGATTAAGGCTTTGTTGTTGGTATTATTTCTCTCTAAATTTTATTTGAATTGTTTTTGTTAAATTATATCCTTTGCTATATATTATTTTGAAAAATATTAATAATAATCCATCGTTATTAGTAATAAGAACGAAAGGAAAATATTGGATTGCCAAAACAATTTCATTACATAATTAGTAATTACAATGTTAATCAATTTCTCTATGATCGTTATTATTTCATACAGAACATGTTCACAGCTGGAACCGACACGTCAGCAAGCACGGTGGAATGGGCCTTGACAGAGCTCATTAGACATCCTGAAATGATGGCCCAGGCCCAACAAGAACTGGACTCCATAGTCGGTCGTGATCGGGCAGTTTCCGATGTAGACCTACATCAACTAGTCTATCTCCAGGCCGTGGTGAAGGAAACTTTCCGGCTCCACCCACCGACGCCACTCTCCCTCCCTCGCATGGCGTCCGACAGCTGCGAGGTCAATGGATATCACATCCCCAAAGGCTCCACTCTCTTAGTCGACGTATGGGCCATCGGTCGTGACCCAAAACAATGGGTCGACCCACTCGAGTTCAGGCCCAATCGGTTCCTCCCCAACGGCGAGAAGCCCCACGTGGATGTGAAGGGAAATGACTTTGAGGTGATTCCGTTTGGGGCTGGGCGGAGAATCTGTGTGGGTTTGAGTTTGGGCTTGAGGATGGTCCAGATGTTAACAGCCACGATAGTCCATTCATTTGATTGGACCTTGCCTAATGGGCTTACACCTGATAAGTTGAACATGGATGAGCATTATGGGCTTACTCTTCGCCGGGCCCAGCCTTTGATCATGCATCCACGACCGAGGTTATTACTTGGGGTTTATGAAAGGGATAGTTACGAATATAGCAACTAAATTCAAAATAATTAAGTATATAACAACATTTTAAAAAAATTGCAAATATAATAAAACCTATCAATGATAGACTATGTAGCAAATATTGGTCTATCACTGATAAACCACAATCGTCTATCAGTGATAAAAGTTATCACCGATAGAATTTGCTATGTTTGTAGTTTTTTTAAAATATTGCTATGTGCTCGATATTTATTTCAAAAATTGCTATCCATTATAATTATCCTTTATAAAACATTGTCTTATATTGGTTAGTTTTAACTTCTTCTTTTCTCATCGGTTGGGTTTAAGTTTTCTTCTTAAGTTTCGTTTTAAAAAATAGTAATAATAGTTTGGGTGAATTGTAAAAACTATCCCTAAAGTATTGTGGTAGCCCTCGACCTTATTCAAGTTCTAAAGGAGACCCTAAAATTTCAAATTTATATAAAATCACATGTATGTTAGTGCTCATATAAGTTTGAACGTTTGATTTTAACAATTTTACAAGTTTAGGGGTCCAATTATTATTATTGAAAGTTTGGGCGTAACTGTAACTAACACCTTACTTTAATGATAATTGTTGTAAAACACAAATAGTTTACGAGTGATTGTGCAATAGGTCTCTAGTTGGTTTATTATAATATTAATCGATTAATTGTGC

mRNA sequence

TTCCATGGAGGAAATTACCCCAATCGTCGCCGCCCTTATAACACTCGCCTCCGCCTTCCTCCTCCACCGTGCTTTCTTCCTCCGCCGTAGACTCCGCCTCCCGCCTGGTCCGAAACCATGGCCGATAGTAGGAAACATGCCGCAGATCGGCGAAGCGCATCTCCACTCTCTCCTTGCCACCATGGCCGGAAAGTACGGACCACTGATGTACCTCCGCTTAGGCTCCGTGGACATGGTCGTAGCGGCATCCGCGGCTGTGGCAGAGCAAGTTTTGAAGATTCACGACACTAATTTCCTTAGCCGGCCACCAAACGCCGGCGCAAAATATATTGCTTATAATTATCAGGATATGGTTTTTGCACCGTACGGTCCACGGTGGCGATTGCTCCGGAAGATCAGCACCGTCCATCTCTTCTCATCAAAAGCCCTAGATGATCATCGCCGCATACGGGAGGAAGAGATATCGGTGCTAGTACAGGCCCTTGCAAGGTCCGGTGAAGCTCCAGCGAACTTGGGGAGCTTGCTCACCGTGTGCATTGCGAACTCCCTTGGACGGACAATGATTGGCCGACGTGTTTTCGGTGATGGAAGCGGTAGCGATGACTTAGAGTCAAATCAATTTAAGTTAATGGTGGAGCAAGTGATGGTGCTGGCAGGAAAGTTCAACCCTGGTGACTTTTTCCCCTGGCTTGAGTGGCTTGATTTGATGGGTGTGGGCAGAGAGATGAAGAAGGTCCATAAATGGTTCGATGATTTTTTGACTAAGATCGTGGAGGAGCATCGGAATTTGTTGGCCAGAGGTGTCGGTGGTGGTCACCAAGATCTGTTGAGTACTCTTCTGTCAATGAAGGATGACGGTGAAGATGAGAATGAGAAGCTCAATGATACTGAGATTAAGGCTTTGTTGTTGAACATGTTCACAGCTGGAACCGACACGTCAGCAAGCACGGTGGAATGGGCCTTGACAGAGCTCATTAGACATCCTGAAATGATGGCCCAGGCCCAACAAGAACTGGACTCCATAGTCGGTCGTGATCGGGCAGTTTCCGATGTAGACCTACATCAACTAGTCTATCTCCAGGCCGTGGTGAAGGAAACTTTCCGGCTCCACCCACCGACGCCACTCTCCCTCCCTCGCATGGCGTCCGACAGCTGCGAGGTCAATGGATATCACATCCCCAAAGGCTCCACTCTCTTAGTCGACGTATGGGCCATCGGTCGTGACCCAAAACAATGGGTCGACCCACTCGAGTTCAGGCCCAATCGGTTCCTCCCCAACGGCGAGAAGCCCCACGTGGATGTGAAGGGAAATGACTTTGAGGTGATTCCGTTTGGGGCTGGGCGGAGAATCTGTGTGGGTTTGAGTTTGGGCTTGAGGATGGTCCAGATGTTAACAGCCACGATAGTCCATTCATTTGATTGGACCTTGCCTAATGGGCTTACACCTGATAAGTTGAACATGGATGAGCATTATGGGCTTACTCTTCGCCGGGCCCAGCCTTTGATCATGCATCCACGACCGAGGTTATTACTTGGGGTTTATGAAAGGGATAGTTACGAATATAGCAACTAAATTCAAAATAATTAAGTATATAACAACATTTTAAAAAAATTGCAAATATAATAAAACCTATCAATGATAGACTATGTAGCAAATATTGGTCTATCACTGATAAACCACAATCGTCTATCAGTGATAAAAGTTATCACCGATAGAATTTGCTATGTTTGTAGTTTTTTTAAAATATTGCTATGTGCTCGATATTTATTTCAAAAATTGCTATCCATTATAATTATCCTTTATAAAACATTGTCTTATATTGGTTAGTTTTAACTTCTTCTTTTCTCATCGGTTGGGTTTAAGTTTTCTTCTTAAGTTTCGTTTTAAAAAATAGTAATAATAGTTTGGGTGAATTGTAAAAACTATCCCTAAAGTATTGTGGTAGCCCTCGACCTTATTCAAGTTCTAAAGGAGACCCTAAAATTTCAAATTTATATAAAATCACATGTATGTTAGTGCTCATATAAGTTTGAACGTTTGATTTTAACAATTTTACAAGTTTAGGGGTCCAATTATTATTATTGAAAGTTTGGGCGTAACTGTAACTAACACCTTACTTTAATGATAATTGTTGTAAAACACAAATAGTTTACGAGTGATTGTGCAATAGGTCTCTAGTTGGTTTATTATAATATTAATCGATTAATTGTGC

Coding sequence (CDS)

ATGGAGGAAATTACCCCAATCGTCGCCGCCCTTATAACACTCGCCTCCGCCTTCCTCCTCCACCGTGCTTTCTTCCTCCGCCGTAGACTCCGCCTCCCGCCTGGTCCGAAACCATGGCCGATAGTAGGAAACATGCCGCAGATCGGCGAAGCGCATCTCCACTCTCTCCTTGCCACCATGGCCGGAAAGTACGGACCACTGATGTACCTCCGCTTAGGCTCCGTGGACATGGTCGTAGCGGCATCCGCGGCTGTGGCAGAGCAAGTTTTGAAGATTCACGACACTAATTTCCTTAGCCGGCCACCAAACGCCGGCGCAAAATATATTGCTTATAATTATCAGGATATGGTTTTTGCACCGTACGGTCCACGGTGGCGATTGCTCCGGAAGATCAGCACCGTCCATCTCTTCTCATCAAAAGCCCTAGATGATCATCGCCGCATACGGGAGGAAGAGATATCGGTGCTAGTACAGGCCCTTGCAAGGTCCGGTGAAGCTCCAGCGAACTTGGGGAGCTTGCTCACCGTGTGCATTGCGAACTCCCTTGGACGGACAATGATTGGCCGACGTGTTTTCGGTGATGGAAGCGGTAGCGATGACTTAGAGTCAAATCAATTTAAGTTAATGGTGGAGCAAGTGATGGTGCTGGCAGGAAAGTTCAACCCTGGTGACTTTTTCCCCTGGCTTGAGTGGCTTGATTTGATGGGTGTGGGCAGAGAGATGAAGAAGGTCCATAAATGGTTCGATGATTTTTTGACTAAGATCGTGGAGGAGCATCGGAATTTGTTGGCCAGAGGTGTCGGTGGTGGTCACCAAGATCTGTTGAGTACTCTTCTGTCAATGAAGGATGACGGTGAAGATGAGAATGAGAAGCTCAATGATACTGAGATTAAGGCTTTGTTGTTGAACATGTTCACAGCTGGAACCGACACGTCAGCAAGCACGGTGGAATGGGCCTTGACAGAGCTCATTAGACATCCTGAAATGATGGCCCAGGCCCAACAAGAACTGGACTCCATAGTCGGTCGTGATCGGGCAGTTTCCGATGTAGACCTACATCAACTAGTCTATCTCCAGGCCGTGGTGAAGGAAACTTTCCGGCTCCACCCACCGACGCCACTCTCCCTCCCTCGCATGGCGTCCGACAGCTGCGAGGTCAATGGATATCACATCCCCAAAGGCTCCACTCTCTTAGTCGACGTATGGGCCATCGGTCGTGACCCAAAACAATGGGTCGACCCACTCGAGTTCAGGCCCAATCGGTTCCTCCCCAACGGCGAGAAGCCCCACGTGGATGTGAAGGGAAATGACTTTGAGGTGATTCCGTTTGGGGCTGGGCGGAGAATCTGTGTGGGTTTGAGTTTGGGCTTGAGGATGGTCCAGATGTTAACAGCCACGATAGTCCATTCATTTGATTGGACCTTGCCTAATGGGCTTACACCTGATAAGTTGAACATGGATGAGCATTATGGGCTTACTCTTCGCCGGGCCCAGCCTTTGATCATGCATCCACGACCGAGGTTATTACTTGGGGTTTATGAAAGGGATAGTTACGAATATAGCAACTAA

Protein sequence

MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYERDSYEYSN*

Homology

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BLAST of CsGy1G002820 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 7.8e-190
Identity = 320/508 (62.99%), Postives = 401/508 (78.94%), Query Frame = 0


Query: 11  LITLASAFLLH---RAFFLRR-RLRLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGP 70
           L T+  +FLL    R+FF +R  L LPPGPKPWPI+GN+  +G    H   A MA  YGP
Sbjct: 8   LYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAAMAQTYGP 67

Query: 71  LMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWR 130
           LMYL++G VD+VVAASA+VA Q LK HD NF SRPPN+GA+++AYNYQD+VFAPYGPRWR
Sbjct: 68  LMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWR 127

Query: 131 LLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIANSLGRTM 190
           +LRKI +VHLFS+KALDD R +R++E+  L +ALA +G+ P  LG LL VC  N+L R M
Sbjct: 128 MLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALARVM 187

Query: 191 IGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHK 250
           +G+RVF DGSG  D ++ +FK MV ++MV+AG FN GDF P L WLD+ GV  +MKK+H 
Sbjct: 188 LGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHA 247

Query: 251 WFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMK-DDGEDENEKLNDTEIKALLLNMF 310
            FD FLT I+EEH+      + G  +DLLSTL+S+K DD +++  KL DTEIKALLLN+F
Sbjct: 248 RFDAFLTDILEEHKG----KIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLNLF 307

Query: 311 TAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKET 370
            AGTDTS+STVEWA+ ELIR+P+++AQAQQE+D +VGRDR V ++DL QL YL+A+VKET
Sbjct: 308 VAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVKET 367

Query: 371 FRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPN 430
           FRLHP TPLSLPR+AS+SCE+NGY IPKGSTLL++VWAI RDP  W DPLEFRP RFLP 
Sbjct: 368 FRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFLPG 427

Query: 431 GEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLN 490
           GEKP VDV+GNDFEVIPFGAGRRIC G++LG+RMVQ++ AT++H+F+W L +G  P+ LN
Sbjct: 428 GEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEMLN 487

Query: 491 MDEHYGLTLRRAQPLIMHPRPRLLLGVY 514
           M+E YGLTL+RA PL++HPRPRL    Y
Sbjct: 488 MEEAYGLTLQRADPLVVHPRPRLEAQAY 510

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BLAST of CsGy1G002820 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 5.8e-185
Identity = 314/501 (62.67%), Postives = 388/501 (77.45%), Query Frame = 0


Query: 18  FLLHRAFFLRRRL----RLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMYLRLG 77
           FL+ R F  RR      RLPPGP PWPI+GN+P +G    H  L+ M   YGP+++LRLG
Sbjct: 15  FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMG-TKPHRTLSAMVTTYGPILHLRLG 74

Query: 78  SVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKIST 137
            VD+VVAAS +VAEQ LKIHD NF SRPPN+GAK++AYNYQD+VFAPYG RWRLLRKIS+
Sbjct: 75  FVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISS 134

Query: 138 VHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIANSLGRTMIGRRVFG 197
           VHLFS+KAL+D + +R+EE+  L + L R G  P NLG L+ +C+ N+LGR MIGRR+FG
Sbjct: 135 VHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREMIGRRLFG 194

Query: 198 DGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLT 257
                 D ++++F+ MV ++M LAG FN GDF P L+WLDL GV  +MK++HK FD FL+
Sbjct: 195 ---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLS 254

Query: 258 KIVEEHRNLLARGVGGGHQDLLSTLLSMK-DDGEDENEKLNDTEIKALLLNMFTAGTDTS 317
            I++EH      G    H D+LSTL+S+K  D + +   L DTEIKALLLNMFTAGTDTS
Sbjct: 255 SILKEHE---MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGTDTS 314

Query: 318 ASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPT 377
           ASTV+WA+ ELIRHP++M +AQ+ELD +VGRDR V++ D+ QL YLQAV+KE FRLHPPT
Sbjct: 315 ASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPT 374

Query: 378 PLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVD 437
           PLSLP +AS+SCE+NGYHIPKGSTLL ++WAI RDP QW DPL F+P RFLP GEK  VD
Sbjct: 375 PLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVD 434

Query: 438 VKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGL 497
           VKG+DFE+IPFGAGRRIC GLSLGLR +Q LTAT+V  FDW L  G+TP+KLNM+E YGL
Sbjct: 435 VKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGL 494

Query: 498 TLRRAQPLIMHPRPRLLLGVY 514
           TL+RA PL++HP+PRL   VY
Sbjct: 495 TLQRAVPLVVHPKPRLAPNVY 508

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BLAST of CsGy1G002820 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 5.8e-169
Identity = 299/501 (59.68%), Postives = 379/501 (75.65%), Query Frame = 0


Query: 14  LASAFLLHRAFFLRRRLR----LPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMY 73
           L S+F+++  +  RRR      LPPGP  WPI+GN+PQ+G A  H  L  ++ +YGPL  
Sbjct: 13  LLSSFIVYLLY--RRRSAQCPPLPPGPNGWPILGNLPQLG-AKPHQTLDALSKQYGPLFR 72

Query: 74  LRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLR 133
           LRLGSV++VVA+S+AVA Q L+ HD NF +RPPN+GA+++AYNYQD+VFAPYGPRWR+LR
Sbjct: 73  LRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLR 132

Query: 134 KISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAP--ANLGSLLTVCIANSLGRTMI 193
           K+ +VHLFS KALDD R +R+ E++ LV+ L R  +     NLG  L VC  N+L R M+
Sbjct: 133 KLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARAML 192

Query: 194 GRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKW 253
           GRRVF D   +   E+++FK MV ++M LAG FN GDF P L WLDL GV  +MK++H+ 
Sbjct: 193 GRRVFAD-EDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHRR 252

Query: 254 FDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKALLLNMFTA 313
           +D FL +++EE++     G      DLLS L+ +K+   +   KLN+T+IKALLLN+FTA
Sbjct: 253 YDAFLDRVIEENQANAKSG------DLLSVLIRLKEADAEGEIKLNNTDIKALLLNLFTA 312

Query: 314 GTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFR 373
           GTDTS+STVEW L ELIRHP+++ + Q ELDS++GRDR V++ DL  L YLQAVVKETFR
Sbjct: 313 GTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKETFR 372

Query: 374 LHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGE 433
           LHP TPLSLPRMAS+ C VNGY IPK +TLLV+VW+IGRD   W DPLEFRP+RFLP GE
Sbjct: 373 LHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLPGGE 432

Query: 434 KPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMD 493
           + HVDVKGNDFEVIPFGAGRRIC GLSLGLRMVQ +TATIVH++DW+LP G    KL+M+
Sbjct: 433 REHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKLDME 492

Query: 494 EHYGLTLRRAQPLIMHPRPRL 509
           E YGLTL+RA PL++ P PRL
Sbjct: 493 EAYGLTLQRAVPLMVQPIPRL 503

[Close]

BLAST of CsGy1G002820 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 7.6e-161
Identity = 289/527 (54.84%), Postives = 375/527 (71.16%), Query Frame = 0


Query: 3   EITPIVAALITLA-SAFLLHRAFFL---------RRRLRLPPGPKPWPIVGNMPQIGEAH 62
           ++ P+   L +LA SA + +  +FL         R+R  LPPGP+ WP++GN+PQ+G+  
Sbjct: 2   DVVPLPLLLGSLAVSAAVWYLVYFLRGGSGGDAARKRRPLPPGPRGWPVLGNLPQLGDKP 61

Query: 63  LHSLLATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYN 122
            H++ A +A +YGPL  LR G  ++VVAASA VA Q L+ HD NF +RPPN+GA+++AYN
Sbjct: 62  HHTMCA-LARQYGPLFRLRFGCAEVVVAASAPVAAQFLRGHDANFSNRPPNSGAEHVAYN 121

Query: 123 YQDMVFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGS 182
           YQD+VFAPYG RWR LRK+  +HLFS+KALDD R +RE E++++V+ LAR   A   LG 
Sbjct: 122 YQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVREGEVALMVRNLARQQAASVALGQ 181

Query: 183 LLTVCIANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWL 242
              VC  N+L R  IG RVF    G     + +FK MV ++M LAG FN GDF P L WL
Sbjct: 182 EANVCATNTLARATIGHRVFAVDGGEG---AREFKEMVVELMQLAGVFNVGDFVPALRWL 241

Query: 243 DLMGVGREMKKVHKWFDDFLTKIVEEHR-NLLARGVGGGH--QDLLSTLLSMKDDG---E 302
           D  GV  +MK++H+ +D+ +   + E +      GV  G    DLLS LL+   +    +
Sbjct: 242 DPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPDGVAAGEHGNDLLSVLLARMQEEQKLD 301

Query: 303 DENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRA 362
            + EK+ +T+IKALLLN+FTAGTDT++STVEWAL ELIRHP+++ +AQ ELD++VGR R 
Sbjct: 302 GDGEKITETDIKALLLNLFTAGTDTTSSTVEWALAELIRHPDVLKEAQHELDTVVGRGRL 361

Query: 363 VSDVDLHQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGR 422
           VS+ DL +L YL AV+KETFRLHP TPLSLPR A++ CEV+GY IPKG+TLLV+VWAI R
Sbjct: 362 VSESDLPRLPYLTAVIKETFRLHPSTPLSLPREAAEECEVDGYRIPKGATLLVNVWAIAR 421

Query: 423 DPKQWVDPLEFRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTAT 482
           DP QW DPL+++P+RFLP      VDVKG DF +IPFGAGRRIC GLS GLRMV ++TAT
Sbjct: 422 DPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGLIPFGAGRRICAGLSWGLRMVTLMTAT 481

Query: 483 IVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVY 514
           +VH FDWTL NG TPDKLNM+E YGLTL+RA PL++ P PRLL   Y
Sbjct: 482 LVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPLMVQPVPRLLPSAY 524

[Close]

BLAST of CsGy1G002820 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.1e-158
Identity = 271/495 (54.75%), Postives = 367/495 (74.14%), Query Frame = 0


Query: 30  LRLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMYLRLGSVDMVVAASAAVAEQV 89
           L+LPPGPK +P++GN+PQ+G A  H  L   +  YGPLM LRLGSVD+VVA+S +VA Q 
Sbjct: 35  LKLPPGPKGYPVLGNLPQLG-AKPHQALQAFSRVYGPLMRLRLGSVDLVVASSPSVAAQF 94

Query: 90  LKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISTVHLFSSKALDDHRRIR 149
           LK +D+NF +RPPN+GA+++A+NY D+VFAPYGPRWRLLRK+S VHL   KALDD++ +R
Sbjct: 95  LK-NDSNFCARPPNSGAEHMAFNYHDLVFAPYGPRWRLLRKLSAVHLLGPKALDDNQNVR 154

Query: 150 EEEISVLVQAL---ARSGEAPANLGSLLTVCIANSLGRTMIGRRVF-----GDGSGSDDL 209
           EEE++VL + L   +R GE P N+G  + VC  N+L R M+GRRVF     G G   ++ 
Sbjct: 155 EEELAVLARMLYERSRGGE-PVNVGKEMHVCSTNALSRAMMGRRVFEKLAVGGGGVEEEE 214

Query: 210 E---SNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLTKIVEE 269
           E   + +FK MV +VM LAG FN GDF PWL+  D+ GV R+MK+VH+ ++ FL K + E
Sbjct: 215 EMKKAEEFKDMVVEVMTLAGVFNIGDFVPWLKPFDIQGVVRKMKRVHRRYNVFLDKFIAE 274

Query: 270 HRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKALLLNMFTAGTDTSASTVEW 329
            R+        G  DLLS L+  +   +    ++ DT IKAL+LN+ TAGTDTS+ST+EW
Sbjct: 275 CRS----SAKPGANDLLSVLIGQRGKSDGSGGEITDTAIKALVLNLLTAGTDTSSSTIEW 334

Query: 330 ALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPTPLSLPR 389
           ALTELIRHP+++ +AQQE+DS VGRDR V++ D+ +L YLQA+VKE FR+HP TPLSLPR
Sbjct: 335 ALTELIRHPDILKKAQQEIDSAVGRDRLVTESDVPKLPYLQAIVKENFRMHPATPLSLPR 394

Query: 390 MASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVDVKGNDF 449
           M+ + C++ GYHIPK STL V++WA+GRDP  W DP+EFRP+RFLP G+  H++V+GN F
Sbjct: 395 MSIEECDIGGYHIPKNSTLFVNIWAMGRDPSIWPDPMEFRPSRFLPGGQGEHLEVRGNHF 454

Query: 450 EVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQ 509
           E++PFGAGRRIC G S+G+R+V    AT++H+FDW LP GLT +K++M+E +G++L++A 
Sbjct: 455 ELMPFGAGRRICAGTSMGIRVVHSTVATLIHAFDWKLPEGLTAEKIDMEEAFGISLQKAI 514

Query: 510 PLIMHPRPRLLLGVY 514
           PL+ HP PRL    Y
Sbjct: 515 PLMAHPIPRLAPKAY 522

[Close]

BLAST of CsGy1G002820 vs. NCBI nr
Match: XP_004138192.1 (flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Csa_014030 [Cucumis sativus])

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 522/522 (100.00%), Postives = 522/522 (100.00%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL 300
           MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL 300

Query: 301 LLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQA 360
           LLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQA
Sbjct: 301 LLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQA 360

Query: 361 VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN 420
           VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN
Sbjct: 361 VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN 420

Query: 421 RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT 480
           RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT
Sbjct: 421 RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT 480

Query: 481 PDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYERDSYEYSN 522
           PDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYERDSYEYSN
Sbjct: 481 PDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYERDSYEYSN 522

[Close]

BLAST of CsGy1G002820 vs. NCBI nr
Match: XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 487/519 (93.83%), Postives = 499/519 (96.15%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           MEEIT IVAA+ITLASAFLLHR FFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLA M
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIR++EISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFK MVEQVMVLAGKFNPGDFFPWLE LDLMGVG E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGG----GHQDLLSTLLSMKDDGEDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GG    GHQDLLSTLLSMKD+G DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDS+VGRDRAVSD+DL QLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPK+WVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLP 480
           F PNRFLP+GEKPHVDVKGNDFE IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW LP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515
           NGLTPDKLNMDEHYGLTLRRAQPL++HPRPRLLLGVY++
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQK 519

[Close]

BLAST of CsGy1G002820 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 444/516 (86.05%), Postives = 474/516 (91.86%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           M+EI  IVAA++TLAS  LLHR FF R R RLPPGPKPWPIVGNMPQIG A LHSLLA M
Sbjct: 1   MDEIALIVAAVVTLASTILLHRTFFRRSRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AG+YG LMYLRLGSV +VVAASAAVAE++LK+HDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKISTVHLFSSKALDDHRRIREEEI+VLVQALA SGE PA LGSLLTVC AN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVCTAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           +LGRTMIGRRVFGDG   DD E+ QFK MVEQVM+LAGKFNPGDFFP LEWLDL+GV  E
Sbjct: 181 ALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGVASE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GGGHQDLLSTLLS     EDENEK+NDTEIKAL
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGRGGGHQDLLSTLLS---SAEDENEKINDTEIKAL 300

Query: 301 LLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQA 360
           LLNMFTAGTDTSASTVEWALTELIRHP+MMAQAQQELDS+VGRDRAVSD+DL QLVYLQA
Sbjct: 301 LLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLVYLQA 360

Query: 361 VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN 420
           VVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKG+TLLVDVWAIGRDPK+W DPLEFRPN
Sbjct: 361 VVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLEFRPN 420

Query: 421 RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT 480
           RFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+VH FDW LPNGL 
Sbjct: 421 RFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALPNGLL 480

Query: 481 PDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYERD 516
           PD+LNMDE YGLTLRRAQPL++HPRPRLL GVY++ 
Sbjct: 481 PDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKS 513

[Close]

BLAST of CsGy1G002820 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 855 bits (2208), Expect = 1.06e-309
Identity = 418/517 (80.85%), Postives = 461/517 (89.17%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLR---LPPGPKPWPIVGNMPQIGEAHLHSLL 60
           M+EI+   +A ITLAS  LL+R  F +R  R   LPPGPKPWPIVGN+PQIG A LHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+ VLVQALARSGEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFK MVEQVM+L+GKFNPGDFF WLEWLDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 GREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEI 300
             EMKKVHKWFDD ++KI+EEHRNLLA G GG HQDLLSTLLS +D  +DENEKLNDTEI
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVY 360
           KALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDS+VGRDR VSD+DL +L Y
Sbjct: 301 KALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGY 360

Query: 361 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 420
           LQAVVKETFRLHPPTPLSLPRMA++SCE++GYHIPKGSTLLVDVWAI RDPK+W  PLEF
Sbjct: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLEF 420

Query: 421 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 480
           RP+RFLP GEK HVDVKGNDF VIPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW LP+
Sbjct: 421 RPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPS 480

Query: 481 GLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYE 514
           GL+P +LNMDE +GLTLRRAQPL+ HPRPRL  GVY+
Sbjct: 481 GLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

[Close]

BLAST of CsGy1G002820 vs. NCBI nr
Match: KAG6587576.1 (Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858 bits (2216), Expect = 1.90e-309
Identity = 418/517 (80.85%), Postives = 462/517 (89.36%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLR---LPPGPKPWPIVGNMPQIGEAHLHSLL 60
           M+EI+   +A ITLAS  LL+R  F +R  R   LPPGPKPWPIVGN+PQIG A LHSLL
Sbjct: 92  MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 151

Query: 61  ATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 152 ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 211

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+ VLVQALAR GEAPA LGSLLTVC
Sbjct: 212 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARPGEAPATLGSLLTVC 271

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFK MVEQVM+L+GKFNPGDFFPWLEWLDL+GV
Sbjct: 272 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPWLEWLDLLGV 331

Query: 241 GREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEI 300
            REMKKVHKWFDD ++KI+EEHRNLLA G GG HQDLL+TLLS +D  +DENEKLNDTEI
Sbjct: 332 AREMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLTTLLSTRDHAKDENEKLNDTEI 391

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVY 360
           KALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDS+VGRDR VSD+DL +L Y
Sbjct: 392 KALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGY 451

Query: 361 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 420
           LQAVVKETFRLHPPTPLSLPRMA++SCE++GYHIPKGSTLLVDVWAI RDPK+W  PLEF
Sbjct: 452 LQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLEF 511

Query: 421 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 480
           RP+RFLP GEK HVDVKGNDF VIPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW LP+
Sbjct: 512 RPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPS 571

Query: 481 GLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYE 514
           GL+P +LNMDE +GLTLRRAQPL+ HPRPRL  GVY+
Sbjct: 572 GLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 608

[Close]

BLAST of CsGy1G002820 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 487/519 (93.83%), Postives = 499/519 (96.15%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           MEEIT IVAA+ITLASAFLLHR FFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLA M
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIR++EISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFK MVEQVMVLAGKFNPGDFFPWLE LDLMGVG E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGG----GHQDLLSTLLSMKDDGEDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GG    GHQDLLSTLLSMKD+G DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDS+VGRDRAVSD+DL QLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPK+WVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLP 480
           F PNRFLP+GEKPHVDVKGNDFE IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW LP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515
           NGLTPDKLNMDEHYGLTLRRAQPL++HPRPRLLLGVY++
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQK 519

[Close]

BLAST of CsGy1G002820 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 487/519 (93.83%), Postives = 499/519 (96.15%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           MEEIT IVAA+ITLASAFLLHR FFLRRR RLPPGPKPWPIVGNMPQIG A LHSLLA M
Sbjct: 1   MEEITLIVAAIITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAAM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKIS VHLFSSKALDDHRRIR++EISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFK MVEQVMVLAGKFNPGDFFPWLE LDLMGVG E
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGSE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGG----GHQDLLSTLLSMKDDGEDENEKLNDTE 300
           MKKVHKWFDDFLTKIVEEHRNLLARG GG    GHQDLLSTLLSMKD+G DENEKLNDTE
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDTE 300

Query: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLV 360
           IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDS+VGRDRAVSD+DL QLV
Sbjct: 301 IKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQLV 360

Query: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLE 420
           YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPK+WVDPLE
Sbjct: 361 YLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPLE 420

Query: 421 FRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLP 480
           F PNRFLP+GEKPHVDVKGNDFE IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDW LP
Sbjct: 421 FGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWALP 480

Query: 481 NGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515
           NGLTPDKLNMDEHYGLTLRRAQPL++HPRPRLLLGVY++
Sbjct: 481 NGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQK 519

[Close]

BLAST of CsGy1G002820 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 938 bits (2424), Expect = 0.0
Identity = 469/506 (92.69%), Postives = 474/506 (93.68%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM
Sbjct: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP
Sbjct: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN
Sbjct: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE
Sbjct: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240

Query: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL 300
           MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL
Sbjct: 241 MKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKAL 300

Query: 301 LLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQA 360
           LL +F +                          +  L+  +GRDRAVSDVDLHQLVYLQA
Sbjct: 301 LLVLFLS--------------------------KFYLNCFIGRDRAVSDVDLHQLVYLQA 360

Query: 361 VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN 420
           VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN
Sbjct: 361 VVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPN 420

Query: 421 RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT 480
           RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT
Sbjct: 421 RFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLT 480

Query: 481 PDKLNMDEHYGLTLRRAQPLIMHPRP 506
           PDKLNMDEHYGLTLRRAQPLIMHPRP
Sbjct: 481 PDKLNMDEHYGLTLRRAQPLIMHPRP 480

[Close]

BLAST of CsGy1G002820 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 5.13e-310
Identity = 418/517 (80.85%), Postives = 461/517 (89.17%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLR---LPPGPKPWPIVGNMPQIGEAHLHSLL 60
           M+EI+   +A ITLAS  LL+R  F +R  R   LPPGPKPWPIVGN+PQIG A LHSLL
Sbjct: 1   MDEISLFFSAAITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSLL 60

Query: 61  ATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GSVD+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  ADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+ VLVQALARSGEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFK MVEQVM+L+GKFNPGDFF WLEWLDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLGV 240

Query: 241 GREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEI 300
             EMKKVHKWFDD ++KI+EEHRNLLA G GG HQDLLSTLLS +D  +DENEKLNDTEI
Sbjct: 241 AGEMKKVHKWFDDLMSKILEEHRNLLATGGGGEHQDLLSTLLSTRDRAKDENEKLNDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVY 360
           KALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDS+VGRDR VSD+DL +L Y
Sbjct: 301 KALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGY 360

Query: 361 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 420
           LQAVVKETFRLHPPTPLSLPRMA++SCE++GYHIPKGSTLLVDVWAI RDPK+W  PLEF
Sbjct: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPLEF 420

Query: 421 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 480
           RP+RFLP GEK HVDVKGNDF VIPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW LP+
Sbjct: 421 RPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPS 480

Query: 481 GLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYE 514
           GL+P +LNMDE +GLTLRRAQPL+ HPRPRL  GVY+
Sbjct: 481 GLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

[Close]

BLAST of CsGy1G002820 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 835 bits (2157), Expect = 2.77e-302
Identity = 412/517 (79.69%), Postives = 456/517 (88.20%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLR---LPPGPKPWPIVGNMPQIGEAHLHSLL 60
           M+EI+   +A ITLA+  LL+R  F +R  R   LPPGPKPWP VGN+PQIG A LHS L
Sbjct: 1   MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQL 60

Query: 61  ATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMV 120
           A M+G+YGPLMYLR GS+D+VVAASAA+AEQ LKI DTNFLSRPPNAGAKYIAYNYQDMV
Sbjct: 61  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120

Query: 121 FAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVC 180
           FAPYGPRWRLLRKIS VHLFSSKAL+D+R +REEE+ VLVQALAR GEAPA LGSLLTVC
Sbjct: 121 FAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVC 180

Query: 181 IANSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGV 240
            AN+LGRTMIGRRVFGDGSG  D  S+QFK MVEQVM+L+GKFNPG+FFP LEWLDL+GV
Sbjct: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGV 240

Query: 241 GREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEI 300
            REMKKVHKWFDD L+KI+EEHR LLA G  G HQDLLSTLLS +D  EDENEKLNDTEI
Sbjct: 241 AREMKKVHKWFDDLLSKILEEHRTLLASG--GEHQDLLSTLLSTRDHAEDENEKLNDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVY 360
           KALLLNMFTAGTDTSASTVEWAL ELIRHP+MM QAQQELDS+VGRDR VSD+DL +L Y
Sbjct: 301 KALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGY 360

Query: 361 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 420
           LQAVVKETFRLHPPTPLSLPRMA++SCE+NGYHIPKGSTLLVDVWAI RDPK+W  PLEF
Sbjct: 361 LQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEF 420

Query: 421 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 480
           RP+RFLP GEK HVDVKGNDF VIPFGAGRRICVG+S+GLRMVQMLTA++VHSFDW LP+
Sbjct: 421 RPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPS 480

Query: 481 GLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYE 514
           GL+P +LNMDE +GLTLRRAQPL+ HPRPRL  GVY+
Sbjct: 481 GLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

[Close]

BLAST of CsGy1G002820 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 648.7 bits (1672), Expect = 4.1e-186
Identity = 314/501 (62.67%), Postives = 388/501 (77.45%), Query Frame = 0


Query: 18  FLLHRAFFLRRRL----RLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMYLRLG 77
           FL+ R F  RR      RLPPGP PWPI+GN+P +G    H  L+ M   YGP+++LRLG
Sbjct: 15  FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMG-TKPHRTLSAMVTTYGPILHLRLG 74

Query: 78  SVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKIST 137
            VD+VVAAS +VAEQ LKIHD NF SRPPN+GAK++AYNYQD+VFAPYG RWRLLRKIS+
Sbjct: 75  FVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISS 134

Query: 138 VHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIANSLGRTMIGRRVFG 197
           VHLFS+KAL+D + +R+EE+  L + L R G  P NLG L+ +C+ N+LGR MIGRR+FG
Sbjct: 135 VHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREMIGRRLFG 194

Query: 198 DGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLT 257
                 D ++++F+ MV ++M LAG FN GDF P L+WLDL GV  +MK++HK FD FL+
Sbjct: 195 ---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLS 254

Query: 258 KIVEEHRNLLARGVGGGHQDLLSTLLSMK-DDGEDENEKLNDTEIKALLLNMFTAGTDTS 317
            I++EH      G    H D+LSTL+S+K  D + +   L DTEIKALLLNMFTAGTDTS
Sbjct: 255 SILKEHE---MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGTDTS 314

Query: 318 ASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPT 377
           ASTV+WA+ ELIRHP++M +AQ+ELD +VGRDR V++ D+ QL YLQAV+KE FRLHPPT
Sbjct: 315 ASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPT 374

Query: 378 PLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVD 437
           PLSLP +AS+SCE+NGYHIPKGSTLL ++WAI RDP QW DPL F+P RFLP GEK  VD
Sbjct: 375 PLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVD 434

Query: 438 VKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGL 497
           VKG+DFE+IPFGAGRRIC GLSLGLR +Q LTAT+V  FDW L  G+TP+KLNM+E YGL
Sbjct: 435 VKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGL 494

Query: 498 TLRRAQPLIMHPRPRLLLGVY 514
           TL+RA PL++HP+PRL   VY
Sbjct: 495 TLQRAVPLVVHPKPRLAPNVY 508

[Close]

BLAST of CsGy1G002820 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 351.7 bits (901), Expect = 1.0e-96
Identity = 187/499 (37.47%), Postives = 281/499 (56.31%), Query Frame = 0


Query: 27  RRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMYLRLGSVDMVVAASAAVA 86
           R R + PPGP P PIVGN+  I            A  YGP++ + +GS+  VV +SA +A
Sbjct: 22  RLRYKFPPGPSPKPIVGNLYDIKPVRFRCYY-EWAQSYGPIISVWIGSILNVVVSSAELA 81

Query: 87  EQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISTVHLFSSKALDDHR 146
           ++VLK HD     R  N   +  + N QD+++A YGP +  +RK+ T+ LF+ K L+  R
Sbjct: 82  KEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLESLR 141

Query: 147 RIREEEISVLVQALARSGEAPAN------LGSLLTVCIANSLGRTMIGRRVFGDGSGSDD 206
            IRE+E++ +V+++ R    P N      L   L     N++ R   G+R F +  G  D
Sbjct: 142 PIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGKR-FMNAEGVVD 201

Query: 207 LESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLTKIVEEHR 266
            +  +FK +V   + L    +  +  PWL W  +     +    H    D LT+ + E  
Sbjct: 202 EQGLEFKAIVSNGLKLGASLSIAEHIPWLRW--MFPADEKAFAEHGARRDRLTRAIMEEH 261

Query: 267 NLLARGVGGGHQDLLSTLLSMKDDGEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWAL 326
            L  +   G  Q  +  LL++KD  +     L++  I  LL +M TAG DT+A T EWA+
Sbjct: 262 TLARQKSSGAKQHFVDALLTLKDQYD-----LSEDTIIGLLWDMITAGMDTTAITAEWAM 321

Query: 327 TELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPTPLSLPRMA 386
            E+I++P +  + Q+E D +VG DR +++ D  +L YLQ VVKE+FRLHPPTPL LP  +
Sbjct: 322 AEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRLHPPTPLMLPHRS 381

Query: 387 SDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVDVKGNDFEV 446
           +   ++ GY IPKGS + V+VWA+ RDP  W +P EFRP RFL    +  VD+KG+DF +
Sbjct: 382 NADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFL----EEDVDMKGHDFRL 441

Query: 447 IPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQPL 506
           +PFGAGRR+C G  LG+ +V  + + ++H F WT P G  P++++M E+ GL      P+
Sbjct: 442 LPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPV 501

Query: 507 IMHPRPRLLLGVYERDSYE 520
                PRL   +Y+R  Y+
Sbjct: 502 QAVATPRLPSDLYKRVPYD 507

[Close]

BLAST of CsGy1G002820 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 351.3 bits (900), Expect = 1.4e-96
Identity = 190/511 (37.18%), Postives = 297/511 (58.12%), Query Frame = 0


Query: 4   ITPIVAALITLASAFLLHRAFFLRR-RLRLPPGPKPWPIVGNMPQIGEAHLHSLLATMAG 63
           +TP   A++ L + F +    F R  +  LPPGP+  PIVGN+P + +  LH+    +A 
Sbjct: 15  LTPYAYAVLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAQ 74

Query: 64  KYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYG 123
            +GP+  L LGS   VV  S ++A ++LK  D NF +      A+ + Y   D+V+ PYG
Sbjct: 75  SHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYG 134

Query: 124 PRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSG--EAPANLGSLLTVCIAN 183
             WR+LRK+    LFS K LD    +R +EI    + L + G  ++P N+G  L + + N
Sbjct: 135 AEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMN 194

Query: 184 SLGRTMIGRRVFGDGSGSDDLES--NQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVG 243
                ++   ++G    ++D+ES   +FK ++ ++  L G  N  DFFP L   DL G+ 
Sbjct: 195 -----LMMNMLWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLV 254

Query: 244 REMKKVHKWFDDFLTKIVEEHRNLLAR-GVGGGHQDLLSTLLSMKDDGEDENEKLNDTEI 303
           ++M    +  D  L + +E+ + L +R G  G  +D L  L+ ++D   D +  +    +
Sbjct: 255 KKMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHV 314

Query: 304 KALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVY 363
           KA+L++M   GT++S +T+E+ + ELI +PE+M +AQQELD +VG+D  V +  +  L Y
Sbjct: 315 KAVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPY 374

Query: 364 LQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEF 423
           + AV+KET RL+P  PL +P   S++  V GY IPK + + ++VW+I RDP  W  P EF
Sbjct: 375 ILAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEF 434

Query: 424 RPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPN 483
           RP RFL   +K   D  G D+  +PFG+GRRIC G++L  RM+    AT++HSFDWT+P+
Sbjct: 435 RPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPD 494

Query: 484 GLTPDKLNMDEHYGLTLRRAQPLIMHPRPRL 509
           G     L+++E +G+ L+   PL+  P PRL
Sbjct: 495 G---HVLDLEEKFGIVLKLKTPLVALPIPRL 513

[Close]

BLAST of CsGy1G002820 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 347.8 bits (891), Expect = 1.5e-95
Identity = 190/523 (36.33%), Postives = 297/523 (56.79%), Query Frame = 0


Query: 1   MEEITPIVAALITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLATM 60
           + +++    +L+ + S F+       RRR   PPGP+ WPI+GNM  + +   H  LA +
Sbjct: 9   LSKLSDPTTSLVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQL-THRGLANL 68

Query: 61  AGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFAP 120
           A KYG L +LR+G + M   +S  VA QVL++ D+ F +RP      Y+ Y+  DM FA 
Sbjct: 69  AKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAH 128

Query: 121 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIAN 180
           YGP WR +RK+  + +FS K  +    +R +E+  +V++++ +   P N+G  +      
Sbjct: 129 YGPFWRQMRKVCVMKVFSRKRAESWASVR-DEVDKMVRSVSCNVGKPINVGEQIF----- 188

Query: 181 SLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGRE 240
           +L R +  R  FG      +   ++F  ++++   L G FN  DF P+  W+D  G+ + 
Sbjct: 189 ALTRNITYRAAFGSAC---EKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKR 248

Query: 241 MKKVHKWFDDFLTKIVEEH--RNLLARGVGGG---HQDLLSTLLS--------MKDDGED 300
           + K     D F+  I++EH  +      V  G     D++  LL+        + +  + 
Sbjct: 249 LVKARNDLDGFIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADL 308

Query: 301 ENE-KLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRA 360
           +N  KL    IKA+++++   GT+T AS +EWALTEL+R PE + + QQEL  +VG DR 
Sbjct: 309 QNSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRR 368

Query: 361 VSDVDLHQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGR 420
           V + D+ +L YL+  +KET R+HPP PL L   A D+  ++G+ IPK S ++++ +AIGR
Sbjct: 369 VEESDIEKLTYLKCTLKETLRMHPPIPLLLHETAEDT-SIDGFFIPKKSRVMINAFAIGR 428

Query: 421 DPKQWVDPLEFRPNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTAT 480
           DP  W DP  FRP+RFL  G     D KG++FE IPFG+GRR C G+ LGL  + +  A 
Sbjct: 429 DPTSWTDPDTFRPSRFLEPGVP---DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAH 488

Query: 481 IVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQPLIMHPRPRLL 510
           I+H F W LP+G+ P +L+M++ +GLT  +A  L   P  RL+
Sbjct: 489 ILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTRLI 517

[Close]

BLAST of CsGy1G002820 vs. TAIR 10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-94
Identity = 185/487 (37.99%), Postives = 277/487 (56.88%), Query Frame = 0


Query: 31  RLPPGPKPWPIVGNMPQIGEAHLHSLLATMAGKYGPLMYLRLGSVDMVVAASAAVAEQVL 90
           RLPPGP   PI+GN+ Q+G    H  LA++  KYGPL+YLRLG+VD +         ++L
Sbjct: 31  RLPPGPPRLPILGNLLQLGPLP-HRDLASLCDKYGPLVYLRLGNVDAITTNDPDTIREIL 90

Query: 91  KIHDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISTVHLFSSKALDDHRRIRE 150
              D  F SRP    A ++AY   D+  AP GP W+ +R+I   HL ++K L+     R 
Sbjct: 91  LRQDDVFSSRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRA 150

Query: 151 EEISVLVQALARSGEA--PANLGSLLTVCIANSLGRTMIGRRVFGDGSGSDDLESNQFKL 210
           EE   L++ + +  E   P NL  +L     N++ R ++G++ FG GS     E+ +F  
Sbjct: 151 EEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFFGPGSLVSPKEAQEFLH 210

Query: 211 MVEQVMVLAGKFNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLTKIVEEHRNL-LARGV 270
           +  ++  L G    GD+ P+  W+D  G  +EM+ V K  D+F TKI++EHR   L    
Sbjct: 211 ITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRVDEFHTKIIDEHRRAKLEDED 270

Query: 271 GGGHQDLLSTLLSMKDDGEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHP 330
             G  D +  LLS+   GE+    + D EIKAL+ +M  A TDTSA T EWA+ E I+ P
Sbjct: 271 KNGDMDFVDVLLSL--PGENGKAHMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIKQP 330

Query: 331 EMMAQAQQELDSIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPTPLSLPRMASDSCEVN 390
            +M + Q+ELD++VG +R V + DL  L YL+ VV+ETFR+HP  P  +P  +  +  +N
Sbjct: 331 RVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATTIN 390

Query: 391 GYHIPKGSTLLVDVWAIGRDPKQWVDPLEFRPNRFLPNGEKPHVDVK-GNDFEVIPFGAG 450
           GY+IP  + + ++   +GR+ K W D  +FRP R  P      V++  G DF+++PF AG
Sbjct: 391 GYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEISHGPDFKILPFSAG 450

Query: 451 RRICVGLSLGLRMVQMLTATIVHSFDWTLPNGLTPDKLNMDEHYGLTLRRAQPLIMHPRP 510
           +R C G  LG+ MV M  A + H F+W+     +P  ++  E YG+T+ +A+PL    +P
Sbjct: 451 KRKCPGAPLGVTMVLMALARLFHCFEWS-----SPGNIDTVEVYGMTMPKAKPLRAIAKP 509

Query: 511 RLLLGVY 514
           RL   +Y
Sbjct: 511 RLAAHLY 509

The following BLAST results are available for this feature:

BLAST of CsGy1G002820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SBQ9	7.8e-190	62.99	Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1	[more]
Q9SD85	5.8e-185	62.67	Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1	[more]
A0A4D6Q415	5.8e-169	59.68	Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN...	[more]
Q7G602	7.6e-161	54.84	Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C...	[more]
A0A4D6Q414	2.1e-158	54.75	Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 G...	[more]

BLAST of CsGy1G002820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138192.1	0.0	100.00	flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Cs...	[more]
XP_008453263.1	0.0	93.83	PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-...	[more]
XP_038878698.1	0.0	86.05	flavonoid 3'-monooxygenase [Benincasa hispida]	[more]
XP_022933611.1	1.06e-309	80.85	flavonoid 3'-monooxygenase [Cucurbita moschata]	[more]
KAG6587576.1	1.90e-309	80.85	Flavonoid 3'-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CsGy1G002820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UTD5	0.0	93.83	Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A1S3BVU8	0.0	93.83	flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1	[more]
A0A0A0LP99	0.0	92.69	Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1	[more]
A0A6J1EZJ7	5.13e-310	80.85	flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV...	[more]
A0A6J1L0D6	2.77e-302	79.69	flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1	[more]

BLAST of CsGy1G002820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07990.1	4.1e-186	62.67	Cytochrome P450 superfamily protein	[more]
AT2G40890.1	1.0e-96	37.47	cytochrome P450, family 98, subfamily A, polypeptide 3	[more]
AT4G12310.1	1.4e-96	37.18	cytochrome P450, family 706, subfamily A, polypeptide 5	[more]
AT4G36220.1	1.5e-95	36.33	ferulic acid 5-hydroxylase 1	[more]
AT1G01280.1	1.7e-94	37.99	cytochrome P450, family 703, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 61..80 score: 35.48 coord: 359..377 score: 40.21 coord: 296..313 score: 32.51 coord: 440..450 score: 57.81 coord: 85..106 score: 22.75 coord: 316..342 score: 33.53 coord: 400..424 score: 37.52 coord: 450..473 score: 24.02
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 307..324 score: 38.24 coord: 360..371 score: 45.39 coord: 441..450 score: 60.9 coord: 450..461 score: 27.07
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..485 e-value: 9.2E-108 score: 360.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 26..508 e-value: 2.5E-131 score: 440.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 33..507
None	No IPR available	PANTHER	PTHR24298:SF45	FLAVONOID 3'-MONOOXYGENASE	coord: 13..505
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 13..505
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 443..452

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy1G002820.2	CsGy1G002820.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016711	flavonoid 3'-monooxygenase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen