CsGy1G001230 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G001230
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic
LocationGy14Chr1: 725618 .. 729949 (+)
RNA-Seq ExpressionCsGy1G001230
SyntenyCsGy1G001230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATAGAGGCATTTTTGAGTGAATGGGTGACCATTGGAATGGGTTTTCTTTTCTTATAAAAAGCAAAAAAGGATTTTATTGTTGAAAATGGTATTTTCAGTTTCCATTATTCTAAGTTTGGGAAAACTTATGAAGGATACTCTTAGATACTTAGCAGGAATTCCAGGCCCCAGTGGCTATGGCTCCAAATCTACTGCTGAACAAGTCTCTCTATTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTCAACTTACTGCCATTATCACTGGTAAATTCTTGTTTACATTTATTTAATTTAATTCTTGTAATTAATTTTGATTTCTTATTTTTCTTTTCATTAGTGTCTCTAATGAGTTTACTCTACTGTGATGTGTAATTCTTGAAGTTTTGAAATTTAAAATCCTTTTCTGATTTTGAAGTTTTGGAGAATCATATTAATATATATATATATATACTGCTAAATTGGGTGAATTATAAATTGTGGTTGGCTTTTGAAGGTTTCTCAGTGGGTTTTTCAAGGTTTTGCATGCATTCAACGTCTCTCTCTCTCTTAAGAGATTTTTTTTTTAGTTCAGAAAGGCTATTTTTTTAGTTGGAAATTTGCTAAATGAAAGGCAGAATTTTGGATAGATAAACAAAAACTCATAGATAGCTTTATAGTGTTTGATAAAGTTTTGATTGTTTATGAATTTTTTATCAGAGCTAAATCTTTTAACTTTTTCATATAATAAGGTTCAAATTTGCCATTAAACCCTATTTTTTTTTTCAAGATTTAGGAAGTGGGATTGTTATTATTATTGTTATTTTTTTATGATAACTATGTTCTTTCATTTGAGGCTCTCATATCAATAGCAGAGATATGATTTTGTCACTTTTCAGATTGTATTTTGTTTCAACACTCACCTGTGTGTGTATATATACCAGCTTTTAACAGCCACACATCAAAAGAATATATATATATATATATATAAATGAAAATTTTAGTCAAATTATATATGTAAATTATTTAGTTTTGATTTGAAAAGGGGCAACATCAGGAATTGGGGCAGAAACAGCAAGAGTATTGGCAAAGAGAGGAGTGAAAATAGTGATGACAGCAAGAGACTTGAAAAAAGCAGCTCAAGTTAAAGAAGCAATTCAAAAAGAAAGCCCTGAGGCTGAGATTATTGTGTTTGAAATCGATCTCAGTTCATTGGCTTCTGTCCAAAGCTTTTGTAATCAGTTTCTTTCTCTTGGACTCCCACTTAACATTCTCATGTAATTCTCTCTCTCTCTCTGCATGTGTACGCATGTAAATCACAAAACTGTTAAAAATACGTTAAAAAATACAGTAAAATTTATAATTTTTTAAATTATCAATAATACACTATTAAATGTCTATCCATAGATCTAAAACTTTTTGTTTTGTTATATATGAAAATGTCCCTATATATAACTTAAAAAATAAAAAATATACAATAAATTATTTTAAATAGTAAAACGGTTGAAAATATTTACAATTATAATAAAATATGGAAGAATATATGTGATAAACCGCAATGATCTAACTGTGATCTATCACGAATTACGAATAGATTGTGTGGAATTTGGTTATATTTATAAATATTTTGGTTGATTTTACCTCGTTTGAAAATAACCTTTAATATATATTATTTATTTTTATACGCAGAAATAATGCTGGAGTCTTCTCCAAGAATTTGGAGTTCTCTGAAGACAAAGTTGAGTTGACATTTGCAACAAATTATTTGGGTATGTCTACATCTTTTGTTACACTATTTATCTTAATTATTATTAATTTTTTAGCTTAGTGTTTATTTGAACATATATATATATATATATATTACTTAACACCCAATATTTGGAAGCAACCCACGTCTTCATACAATGAGTTATCTCAATCCATATGTCAGTACAAAAAAGATGAATAAGAAATAAAAGCCGACCAATTTTCTTATCTAAATTTCTTTAGATACAAGAATATTAAATTTGTCTTTTAACTTATTTGCTTAAAGTTTTAGATGATATCGAAACATCTTAGACAGAAATAAACTTCTTAAAGTCAGAAATTAGGTTTCGAAATCAATTAGAATGGGTTAATGGTATAAGTTTGACAAATAATTACATTACAAAGTGTTAATACCACTTGAAAAGTGATAGAATAGGATTAATTAAGTCCATTGAAATTACCTTTGGAAGAGAGAAAAAAAAAAAGAAGGAGAAGAAAAGGAAAAGACAAACCGACCAGGTGTAAAAGGATATATTAGGGAACCCAACAATGTATGGTGGCGTATCTGGCAGTGGGTCCCGACTTGAGAAAATATGCACCAAACACAAATCAATGTAAAGACAAGTGAAACCTGGGACCCGTGTCCACCCTCGTGTAGCTTTCAAACTAAGTTATAAGTGTTCAAATATAGCAAAAAGAACTACTATCTATATTATATAATAGTCGTTATCTTTCAACGCGATGATAGTAAAAATTTTACTGTATTTGTATTTATAAATATATATTTTTTTATTTTTCAAATTAATCTAGGATTAATTTATAGTTTATAAAGAATTTTTGCTATAATATATATTGTACATGAAAGTTGAAATAAAGCTCCTTTAGACATGAATTTCAATTTCAATTTTATCTTATAAAATTAATTTTATTTTTTATTTAAATAATATAACTTATTGAACACAAAGTTCATAAACTTAATATACTTTTTAAAGGTTTAAAAACAATATAGCTATTTGTTACTTTAAGATTTTAACCTATAAGAGTTGAAGTGTTTCCAAGTAGATAACATTTTCTATCTACCATCTTAAAGATGTATAGCTCCAAAAGCAATTTATTGCAGTTTAGCATGAAACACTCAATTGATGACTTTTCTAAAAGTAACACTACATTCAAGCTAATTCCAACTTAAACTTTTTTACAATATGTGAGGTGAAAGATCAAAACTACGCTTTGGAGTTAAAATTATCATCAATATTGAGAGTTTAAGGTTGGACTATCATTAACATTGAGGGTTACAAATGCAATTATATAATATATGATTTGAATGATTTTTGTTCATCTTCTTTGCAGGACATTATTTACTAACAGAGAGGCTGTTAGAGAAAATGATAGAAACAGCAGCCAAGACAGGAATTGAAGGAAGGATTATCAACGTTTCATCGGTGGTTCATGGCTGGGTGAAGAAAGATGGTTTAAGCTTTCGTCAAATGCTCAACCCAAACAGGTATGGCCAATCATATTTAGAGCCTCCTTGCACTAAAAAACCACGTAAGAGCGTGTGCCCTCAAGTGGAGTTAATAAGTTAATAAATCTAAGGTTGATATGTTGGAGGTGATAAGTTTTTGTTCGGAGTGTGGAACAAAAGTTACTCAATAATTGTGAAAAGTAGATTAAGACGAAGTTATTTGGTAAATGTGAAAACTAGAGATAAAAAGGGAAAGATGAAGTTCTTTAGTAAATGTAGAAAGTACTGAAAAATACTAAGATGAATTTACTTAAAAAGTATTAAAAAAATACAACATAAACTGAGTTGTTTATACCTACCTAACAAGTCTGCAAGTCAAACAATCCTATAATGTTTAGGGCAAGGAGTGAAGTTGTTTAGTCTAAAAAGATATCTCATTGGTTGTTTGGTTGTGGTGTCCACAATGTACGTAGTTTAATAAGTCATGAAAGTGTGTTCAAGGTACAAACTATTTTAGTTTGATTATTCGTAAGTACTCGAGTTAACTTACGATTAATCTCACAGAATATCTTATCTAACCCTATAATATTTGAGTGTCAATTAGGATACCATATCACAAGTAGGTGACCACCATAGGTTGAACCAACCTCTTTGTCTTGTATCAAGGTCATACCTCCATATTTATGACTAGGCCAACTTATGATTGGTTAATAATATTATAGTTAATTGTAAGTTAATAAGAATTAAAAACAACGTCTAAAATTGGTCTAAAATTGGAGTTCTAAACATAAAATGAGCTATAATAGTACACTCTACCTATACCCCTAATCCTCTCTCTGCCATGGTGGAAGAAATATTGAAGAATCGTGGTTTATTTGCAGTTACAATGGCACTCGTGCATATGCTCAATCAAAACTAGCCAATATCTTGCATGCTAAGGAACTATCAAGGCAACTTCAGGTAGAGTCTTCCAGTATCATTTCGCATTCTTCCTTATTCAATAAAATGAACCCTCAACCTTATTCCTAAAAGTATCAAATTCTTCAAATACTCAGCTAGCTAACAATTCTAAGAAACATAAGCAATTAAATGAGAAATTGAAAAGAATTCAG

mRNA sequence

CATAGAGGCATTTTTGAGTGAATGGGTGACCATTGGAATGGGTTTTCTTTTCTTATAAAAAGCAAAAAAGGATTTTATTGTTGAAAATGGTATTTTCAGTTTCCATTATTCTAAGTTTGGGAAAACTTATGAAGGATACTCTTAGATACTTAGCAGGAATTCCAGGCCCCAGTGGCTATGGCTCCAAATCTACTGCTGAACAAGTCTCTCTATTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTCAACTTACTGCCATTATCACTGGGGCAACATCAGGAATTGGGGCAGAAACAGCAAGAGTATTGGCAAAGAGAGGAGTGAAAATAGTGATGACAGCAAGAGACTTGAAAAAAGCAGCTCAAGTTAAAGAAGCAATTCAAAAAGAAAGCCCTGAGGCTGAGATTATTGTGTTTGAAATCGATCTCAGTTCATTGGCTTCTGTCCAAAGCTTTTGTAATCAGTTTCTTTCTCTTGGACTCCCACTTAACATTCTCATAAATAATGCTGGAGTCTTCTCCAAGAATTTGGAGTTCTCTGAAGACAAAGTTGAGTTGACATTTGCAACAAATTATTTGGGACATTATTTACTAACAGAGAGGCTGTTAGAGAAAATGATAGAAACAGCAGCCAAGACAGGAATTGAAGGAAGGATTATCAACGTTTCATCGGTGGTTCATGGCTGGGTGAAGAAAGATGGTTTAAGCTTTCGTCAAATGCTCAACCCAAACAGTTACAATGGCACTCGTGCATATGCTCAATCAAAACTAGCCAATATCTTGCATGCTAAGGAACTATCAAGGCAACTTCAGGTAGAGTCTTCCAGTATCATTTCGCATTCTTCCTTATTCAATAAAATGAACCCTCAACCTTATTCCTAAAAGTATCAAATTCTTCAAATACTCAGCTAGCTAACAATTCTAAGAAACATAAGCAATTAAATGAGAAATTGAAAAGAATTCAG

Coding sequence (CDS)

ATGGTATTTTCAGTTTCCATTATTCTAAGTTTGGGAAAACTTATGAAGGATACTCTTAGATACTTAGCAGGAATTCCAGGCCCCAGTGGCTATGGCTCCAAATCTACTGCTGAACAAGTCTCTCTATTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTCAACTTACTGCCATTATCACTGGGGCAACATCAGGAATTGGGGCAGAAACAGCAAGAGTATTGGCAAAGAGAGGAGTGAAAATAGTGATGACAGCAAGAGACTTGAAAAAAGCAGCTCAAGTTAAAGAAGCAATTCAAAAAGAAAGCCCTGAGGCTGAGATTATTGTGTTTGAAATCGATCTCAGTTCATTGGCTTCTGTCCAAAGCTTTTGTAATCAGTTTCTTTCTCTTGGACTCCCACTTAACATTCTCATAAATAATGCTGGAGTCTTCTCCAAGAATTTGGAGTTCTCTGAAGACAAAGTTGAGTTGACATTTGCAACAAATTATTTGGGACATTATTTACTAACAGAGAGGCTGTTAGAGAAAATGATAGAAACAGCAGCCAAGACAGGAATTGAAGGAAGGATTATCAACGTTTCATCGGTGGTTCATGGCTGGGTGAAGAAAGATGGTTTAAGCTTTCGTCAAATGCTCAACCCAAACAGTTACAATGGCACTCGTGCATATGCTCAATCAAAACTAGCCAATATCTTGCATGCTAAGGAACTATCAAGGCAACTTCAGGTAGAGTCTTCCAGTATCATTTCGCATTCTTCCTTATTCAATAAAATGAACCCTCAACCTTATTCCTAA

Protein sequence

MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVESSSIISHSSLFNKMNPQPYS*
Homology
BLAST of CsGy1G001230 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 6.0e-48
Identity = 115/241 (47.72%), Postives = 154/241 (63.90%), Query Frame = 0

Query: 22  LAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATSGIGAETA 81
           + G  G SGYGS STAE V                + S     LTAIITG TSGIG E A
Sbjct: 7   VTGKAGKSGYGSASTAEDV----------------THSIDAKHLTAIITGGTSGIGLEAA 66

Query: 82  RVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFCNQFLSLG 141
           RVL  RG  +++ AR+ K A   KE I +  P A I   ++DLSS+ SV+SF +QFL+L 
Sbjct: 67  RVLGMRGAHVIIAARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALN 126

Query: 142 LPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKTGIEGRII 201
           +PLNILINNAGV     + SED +E  FATN++GH+LLT  LL+KM  +A ++GIEGRI+
Sbjct: 127 VPLNILINNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIV 186

Query: 202 NVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVESSSIISH 261
           N+SS+ H +   +G+ F  + +P+ Y+  +AY QSKLAN+LH+  LSR+LQ E  +I  +
Sbjct: 187 NLSSIAHTYTYTEGIMFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITIN 231

Query: 262 S 263
           S
Sbjct: 247 S 231

BLAST of CsGy1G001230 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.3e-47
Identity = 114/241 (47.30%), Postives = 154/241 (63.90%), Query Frame = 0

Query: 22  LAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATSGIGAETA 81
           + G  G SGYGS STAE V                + S     LTAIITG TSGIG E A
Sbjct: 7   VTGKAGKSGYGSASTAEDV----------------THSIDAKHLTAIITGGTSGIGLEAA 66

Query: 82  RVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFCNQFLSLG 141
           RVL  RG  +++ +R+ K A   KE I +  P A I   ++DLSS+ SV+SF +QFL+L 
Sbjct: 67  RVLGMRGAHVIIASRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALN 126

Query: 142 LPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKTGIEGRII 201
           +PLNILINNAGV     + SED +E  FATN++GH+LLT  LL+KM  +A ++GIEGRI+
Sbjct: 127 VPLNILINNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIV 186

Query: 202 NVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVESSSIISH 261
           N+SS+ H +   +G+ F  + +P+ Y+  +AY QSKLAN+LH+  LSR+LQ E  +I  +
Sbjct: 187 NLSSIAHTYTYTEGIMFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITIN 231

Query: 262 S 263
           S
Sbjct: 247 S 231

BLAST of CsGy1G001230 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.3e-46
Identity = 109/239 (45.61%), Postives = 159/239 (66.53%), Query Frame = 0

Query: 24  GIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATSGIGAETARV 83
           G  G SG+ S+STAE+V                +     + LTAI+TGA+SGIG ETARV
Sbjct: 5   GSKGASGFSSRSTAEEV----------------THGVDGTGLTAIVTGASSGIGVETARV 64

Query: 84  LAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFCNQFLSLGLP 143
           L+ RGV +VM  R+    A+VKE I K+ P A++ V E+DLSS+ SV+ F +++ S GLP
Sbjct: 65  LSLRGVHVVMAVRNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLP 124

Query: 144 LNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKTGIEGRIINV 203
           LN+LINNAG+ +     S+D +EL FATN+LGH+LLT+ LL+ M  T+ ++  EGRI+N+
Sbjct: 125 LNLLINNAGIMACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNL 184

Query: 204 SSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVESSSIISHS 263
           SS  H +   +G+ F ++ + +SY+  RAY QSKL N+LHA EL++QL+ +  +I ++S
Sbjct: 185 SSEAHRFSYPEGVRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANS 227

BLAST of CsGy1G001230 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.0e-45
Identity = 106/236 (44.92%), Postives = 153/236 (64.83%), Query Frame = 0

Query: 27  GPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATSGIGAETARVLAK 86
           G SG+   STAEQV                +     + LTAI+TGA+SGIGAET RVLA 
Sbjct: 9   GVSGFSGSSTAEQV----------------THGIDATGLTAIVTGASSGIGAETTRVLAL 68

Query: 87  RGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFCNQFLSLGLPLNI 146
           RG  ++M  R++  A  VK+ I K+ P A++   E+DLSSL SV+ F ++F S G PLNI
Sbjct: 69  RGAHVIMGVRNMVAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNI 128

Query: 147 LINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKTGIEGRIINVSSV 206
           LINNAG+ +   + S+D +EL FATN++GH+LLT  LL+ M +T  ++  EGRI+NV+S 
Sbjct: 129 LINNAGIMACPFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASE 188

Query: 207 VHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVESSSIISHS 263
            H +   +G+ F ++ + +SYN  RAY QSKLAN+LHA +L++ L+ +  +I ++S
Sbjct: 189 AHRFAYPEGIRFDKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANS 228

BLAST of CsGy1G001230 vs. ExPASy Swiss-Prot
Match: Q8BYK4 (Retinol dehydrogenase 12 OS=Mus musculus OX=10090 GN=Rdh12 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 8.4e-26
Identity = 71/185 (38.38%), Postives = 110/185 (59.46%), Query Frame = 0

Query: 68  IITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSL 127
           +ITGA +GIG ETAR LA+RG ++ +  RD+ K       I+ ++  ++++V ++DLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDT 102

Query: 128 ASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKM 187
            S+++F  +FL+    L+ILINNAGV       + D  E  F  N+LGH+LLT  LLE++
Sbjct: 103 KSIRAFAERFLAEEKKLHILINNAGVMMCPYSKTTDGFETHFGVNHLGHFLLTYLLLERL 162

Query: 188 IETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKEL 247
            E+A       R++N+SS+ H   K   + F  +     Y    AY  SKLAN+L  +EL
Sbjct: 163 KESA-----PARVVNLSSIAHLIGK---IRFHDLQGQKRYCSAFAYGHSKLANLLFTREL 219

Query: 248 SRQLQ 253
           +++LQ
Sbjct: 223 AKRLQ 219

BLAST of CsGy1G001230 vs. NCBI nr
Match: XP_004138098.3 (short-chain dehydrogenase TIC 32 B, chloroplastic [Cucumis sativus] >KGN63560.2 hypothetical protein Csa_013220 [Cucumis sativus])

HSP 1 Score: 451 bits (1160), Expect = 5.62e-157
Identity = 252/258 (97.67%), Postives = 255/258 (98.84%), Query Frame = 0

Query: 1   MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS 60
           MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS
Sbjct: 1   MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS 60

Query: 61  STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 120
           STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF
Sbjct: 61  STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 120

Query: 121 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 180
           EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT
Sbjct: 121 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 180

Query: 181 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN 240
           ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN
Sbjct: 181 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN 240

Query: 241 ILHAKELSRQLQVESSSI 258
           ILHAKELSRQLQ  ++ +
Sbjct: 241 ILHAKELSRQLQGRNARV 258

BLAST of CsGy1G001230 vs. NCBI nr
Match: XP_008464537.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Cucumis melo])

HSP 1 Score: 426 bits (1096), Expect = 3.76e-147
Identity = 237/258 (91.86%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS 60
           MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLF             SSSS
Sbjct: 20  MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLF-------------SSSS 79

Query: 61  STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 120
           S+SQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF
Sbjct: 80  SSSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 139

Query: 121 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 180
           EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT
Sbjct: 140 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 199

Query: 181 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN 240
           ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLAN
Sbjct: 200 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLAN 259

Query: 241 ILHAKELSRQLQVESSSI 258
           ILHAKELSRQLQ  ++ +
Sbjct: 260 ILHAKELSRQLQGRNARV 264

BLAST of CsGy1G001230 vs. NCBI nr
Match: KAA0057835.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 407 bits (1046), Expect = 4.45e-140
Identity = 223/244 (91.39%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           MKDTLRYLAGIPGPSGYGSKSTAEQVSLF               SSS+SQLTAIITGATS
Sbjct: 1   MKDTLRYLAGIPGPSGYGSKSTAEQVSLF---------------SSSSSQLTAIITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC
Sbjct: 61  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
           NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT
Sbjct: 121 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVE 254
           GIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKELSRQLQVE
Sbjct: 181 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKELSRQLQVE 229

Query: 255 SSSI 258
           ++ +
Sbjct: 241 NARV 229

BLAST of CsGy1G001230 vs. NCBI nr
Match: TYJ98519.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 405 bits (1040), Expect = 3.90e-139
Identity = 223/244 (91.39%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           MKDTLRYLAGIPGPSGYGSKSTAEQVSLF             SSSSS+SQLTAIITGATS
Sbjct: 1   MKDTLRYLAGIPGPSGYGSKSTAEQVSLF-------------SSSSSSSQLTAIITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC
Sbjct: 61  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
           NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT
Sbjct: 121 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVE 254
           GIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKELSRQLQ  
Sbjct: 181 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKELSRQLQGR 231

Query: 255 SSSI 258
           ++ +
Sbjct: 241 NARV 231

BLAST of CsGy1G001230 vs. NCBI nr
Match: KAG7023113.1 (Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394 bits (1013), Expect = 8.08e-135
Identity = 221/258 (85.66%), Postives = 231/258 (89.53%), Query Frame = 0

Query: 1   MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS 60
           MVFSVSIILSLGKLMKDTLRYLAGI GPSGYGSKSTAEQV+               SS S
Sbjct: 1   MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQC-------------SSFS 60

Query: 61  STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 120
           STSQLTAIITGATSGIGAETARVLAKRGV+IVM ARD+KKAAQVKEAIQ+ESPEAEIIV 
Sbjct: 61  STSQLTAIITGATSGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVC 120

Query: 121 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 180
           EIDLSSLASVQSFCN FL+L LPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LT
Sbjct: 121 EIDLSSLASVQSFCNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLT 180

Query: 181 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN 240
           E LLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSF QMLNPN+YNGTRAYAQSKLAN
Sbjct: 181 ETLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLAN 240

Query: 241 ILHAKELSRQLQVESSSI 258
           ILHAKE+SRQLQ  ++ +
Sbjct: 241 ILHAKEVSRQLQGRNARV 245

BLAST of CsGy1G001230 vs. ExPASy TrEMBL
Match: A0A1S3CLP7 (short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502388 PE=3 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 1.82e-147
Identity = 237/258 (91.86%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 1   MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS 60
           MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLF             SSSS
Sbjct: 20  MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLF-------------SSSS 79

Query: 61  STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 120
           S+SQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF
Sbjct: 80  SSSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 139

Query: 121 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 180
           EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT
Sbjct: 140 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 199

Query: 181 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN 240
           ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLAN
Sbjct: 200 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLAN 259

Query: 241 ILHAKELSRQLQVESSSI 258
           ILHAKELSRQLQ  ++ +
Sbjct: 260 ILHAKELSRQLQGRNARV 264

BLAST of CsGy1G001230 vs. ExPASy TrEMBL
Match: A0A0A0LNV3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G004220 PE=3 SV=1)

HSP 1 Score: 423 bits (1088), Expect = 1.44e-146
Identity = 237/244 (97.13%), Postives = 240/244 (98.36%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSS SQLTAIITGATS
Sbjct: 1   MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSS-SQLTAIITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC
Sbjct: 61  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
           NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT
Sbjct: 121 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVE 254
           GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQ  
Sbjct: 181 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQGR 240

Query: 255 SSSI 258
           ++ +
Sbjct: 241 NARV 243

BLAST of CsGy1G001230 vs. ExPASy TrEMBL
Match: A0A5A7UW68 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001220 PE=3 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 2.16e-140
Identity = 223/244 (91.39%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           MKDTLRYLAGIPGPSGYGSKSTAEQVSLF               SSS+SQLTAIITGATS
Sbjct: 1   MKDTLRYLAGIPGPSGYGSKSTAEQVSLF---------------SSSSSQLTAIITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC
Sbjct: 61  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
           NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT
Sbjct: 121 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVE 254
           GIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKELSRQLQVE
Sbjct: 181 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKELSRQLQVE 229

Query: 255 SSSI 258
           ++ +
Sbjct: 241 NARV 229

BLAST of CsGy1G001230 vs. ExPASy TrEMBL
Match: A0A5D3BHM5 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001240 PE=3 SV=1)

HSP 1 Score: 405 bits (1040), Expect = 1.89e-139
Identity = 223/244 (91.39%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           MKDTLRYLAGIPGPSGYGSKSTAEQVSLF             SSSSS+SQLTAIITGATS
Sbjct: 1   MKDTLRYLAGIPGPSGYGSKSTAEQVSLF-------------SSSSSSSQLTAIITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC
Sbjct: 61  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
           NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT
Sbjct: 121 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVE 254
           GIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKELSRQLQ  
Sbjct: 181 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKELSRQLQGR 231

Query: 255 SSSI 258
           ++ +
Sbjct: 241 NARV 231

BLAST of CsGy1G001230 vs. ExPASy TrEMBL
Match: A0A6J1JJF7 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485650 PE=3 SV=1)

HSP 1 Score: 393 bits (1009), Expect = 1.70e-134
Identity = 220/258 (85.27%), Postives = 231/258 (89.53%), Query Frame = 0

Query: 1   MVFSVSIILSLGKLMKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSS 60
           MVFSVSIILSLGKLMKDTLRYLAGI GPSGYGSKSTAEQV+               SS S
Sbjct: 3   MVFSVSIILSLGKLMKDTLRYLAGIAGPSGYGSKSTAEQVTQC-------------SSFS 62

Query: 61  STSQLTAIITGATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVF 120
           STSQLTAIITGATSGIGAETARVLAKRGV+IVM ARD+KKAAQVKEAIQ+ESPEAEIIV 
Sbjct: 63  STSQLTAIITGATSGIGAETARVLAKRGVRIVMPARDMKKAAQVKEAIQRESPEAEIIVC 122

Query: 121 EIDLSSLASVQSFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLT 180
           EIDLSSLASVQSFCN FL+L LPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHY LT
Sbjct: 123 EIDLSSLASVQSFCNHFLALRLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLT 182

Query: 181 ERLLEKMIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLAN 240
           E LLEKMIETAA+TGIEGRIINVSSVVHGWVKKDGLSF QMLNPN+YNGTRAYAQSKLAN
Sbjct: 183 ETLLEKMIETAAQTGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLAN 242

Query: 241 ILHAKELSRQLQVESSSI 258
           ILHAKE+SRQLQ  ++ +
Sbjct: 243 ILHAKEVSRQLQGRNARV 247

BLAST of CsGy1G001230 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 2.9e-82
Identity = 169/248 (68.15%), Postives = 198/248 (79.84%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQV---SLFPSSSSSSSSSSSSSSSSSTSQLTAIITG 74
           MK TLRYLAGI GP+G+GS+STAEQV   S FP                  S LTAIITG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFP-----------------CSHLTAIITG 60

Query: 75  ATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQ 134
            TSGIGAETARVLAKRGV++VM  RD+KKA  VKE I +E+PEA+II+FEIDLSSL+SV 
Sbjct: 61  GTSGIGAETARVLAKRGVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVA 120

Query: 135 SFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETA 194
            FC+QFLS  LPLNILINNAGVFS NLEFSE+K+ELTFATN+LGHYLLTE L+EKMI+TA
Sbjct: 121 RFCSQFLSQDLPLNILINNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTA 180

Query: 195 AKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNS-YNGTRAYAQSKLANILHAKELSRQ 254
            K+GIEGRIIN+SSV+H WVK D  SF ++L+P S YNGTRAYAQSKLA ILHAK LS+Q
Sbjct: 181 EKSGIEGRIINLSSVIHNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQ 231

Query: 255 LQVESSSI 259
           L+  ++++
Sbjct: 241 LKDRNANV 231

BLAST of CsGy1G001230 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 2.9e-82
Identity = 169/248 (68.15%), Postives = 198/248 (79.84%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQV---SLFPSSSSSSSSSSSSSSSSSTSQLTAIITG 74
           MK TLRYLAGI GP+G+GS+STAEQV   S FP                  S LTAIITG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFP-----------------CSHLTAIITG 60

Query: 75  ATSGIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQ 134
            TSGIGAETARVLAKRGV++VM  RD+KKA  VKE I +E+PEA+II+FEIDLSSL+SV 
Sbjct: 61  GTSGIGAETARVLAKRGVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVA 120

Query: 135 SFCNQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETA 194
            FC+QFLS  LPLNILINNAGVFS NLEFSE+K+ELTFATN+LGHYLLTE L+EKMI+TA
Sbjct: 121 RFCSQFLSQDLPLNILINNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTA 180

Query: 195 AKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNS-YNGTRAYAQSKLANILHAKELSRQ 254
            K+GIEGRIIN+SSV+H WVK D  SF ++L+P S YNGTRAYAQSKLA ILHAK LS+Q
Sbjct: 181 EKSGIEGRIINLSSVIHNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQ 231

Query: 255 LQVESSSI 259
           L+  ++++
Sbjct: 241 LKDRNANV 231

BLAST of CsGy1G001230 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.1e-60
Identity = 134/249 (53.82%), Postives = 174/249 (69.88%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           M +T+++L G  GPSG+GS+STA+ V                + +S    LTAIITGATS
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHV----------------TCNSDLRSLTAIITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRG ++V+ AR +K A + K  I  E P+AEIIV  +DLSSL SV+ F 
Sbjct: 61  GIGAETARVLAKRGARLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFV 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
           + F SL LPLNILINNAG ++     SED VE+TFATNYLGH+LLT+ LL+KMIETAA+T
Sbjct: 121 DDFESLNLPLNILINNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQT 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPN--SYNGTRAYAQSKLANILHAKELSRQLQ 254
           G++GRI+NV+SVVH W   D L +   ++ N  +Y+ TRAYA SKLAN+LH  ELSR L 
Sbjct: 181 GVQGRIVNVTSVVHSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLH 233

Query: 255 VESSSIISH 262
              +++ ++
Sbjct: 241 KMDANVTAN 233

BLAST of CsGy1G001230 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 5.0e-58
Identity = 125/240 (52.08%), Postives = 167/240 (69.58%), Query Frame = 0

Query: 15  MKDTLRYLAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATS 74
           M +T +YL G  G SG+GSKSTAE+V                + +     +TA+ITGATS
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEV----------------TENCDLRSITAVITGATS 60

Query: 75  GIGAETARVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFC 134
           GIGAETARVLAKRG +++  AR++K A + KE I  E PE EI+V ++DLSS+ASV++F 
Sbjct: 61  GIGAETARVLAKRGARLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFV 120

Query: 135 NQFLSLGLPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKT 194
             F SL LPLN+LINNAG  +     SED +E+TFATNYLGH+LLT  LL KMI+TA +T
Sbjct: 121 ADFESLDLPLNLLINNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEET 180

Query: 195 GIEGRIINVSSVVHGWVKKDGLSFRQMLNPN--SYNGTRAYAQSKLANILHAKELSRQLQ 253
           G++GRI+NV+S +HGW   D + + ++++     ++ TRAYA SKLAN+LH KELS +LQ
Sbjct: 181 GVQGRIVNVTSGIHGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQ 224

BLAST of CsGy1G001230 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 3.8e-50
Identity = 115/241 (47.72%), Postives = 158/241 (65.56%), Query Frame = 0

Query: 22  LAGIPGPSGYGSKSTAEQVSLFPSSSSSSSSSSSSSSSSSTSQLTAIITGATSGIGAETA 81
           + G  GPSG+GS STAE+V                +     + LTAIITG T GIG ETA
Sbjct: 7   ITGRRGPSGFGSASTAEEV----------------TQGIDATNLTAIITGGTGGIGMETA 66

Query: 82  RVLAKRGVKIVMTARDLKKAAQVKEAIQKESPEAEIIVFEIDLSSLASVQSFCNQFLSLG 141
           RVL+KRG  +V+ AR++  A   K  I +++  A + + ++DLSS+ S+++F  +F +L 
Sbjct: 67  RVLSKRGAHVVIGARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALH 126

Query: 142 LPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYLLTERLLEKMIETAAKTGIEGRII 201
           LPLN+LINNAGV     + SED +EL FATN++GH+LLT  LL+ M  TA  +G+EGRI+
Sbjct: 127 LPLNLLINNAGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRIL 186

Query: 202 NVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKELSRQLQVESSSIISH 261
           NVSSV H +  ++G+ F  + +  SY+  RAY QSKLANILHA ELSRQLQ E  +I ++
Sbjct: 187 NVSSVAHIYTYQEGIQFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITAN 231

Query: 262 S 263
           S
Sbjct: 247 S 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A078ISJ66.0e-4847.72Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078IS661.3e-4747.30Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A2RVM04.3e-4645.61Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV48.0e-4544.92Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
Q8BYK48.4e-2638.38Retinol dehydrogenase 12 OS=Mus musculus OX=10090 GN=Rdh12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004138098.35.62e-15797.67short-chain dehydrogenase TIC 32 B, chloroplastic [Cucumis sativus] >KGN63560.2 ... [more]
XP_008464537.13.76e-14791.86PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Cucumis melo][more]
KAA0057835.14.45e-14091.39short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa][more]
TYJ98519.13.90e-13991.39short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa][more]
KAG7023113.18.08e-13585.66Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
A0A1S3CLP71.82e-14791.86short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LNV31.44e-14697.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G004220 PE=3 SV=1[more]
A0A5A7UW682.16e-14091.39Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3BHM51.89e-13991.39Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1JJF71.70e-13485.27short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max... [more]
Match NameE-valueIdentityDescription
AT5G50130.22.9e-8268.15NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G50130.12.9e-8268.15NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G64590.13.1e-6053.82NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24050.15.0e-5852.08NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02540.13.8e-5047.72NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 66..83
score: 45.52
coord: 192..208
score: 33.17
coord: 142..153
score: 47.12
coord: 233..252
score: 26.68
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 142..153
score: 46.66
coord: 198..206
score: 61.15
coord: 233..252
score: 25.42
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 66..207
e-value: 5.5E-30
score: 104.3
NoneNo IPR availableGENE3D3.40.50.720coord: 51..266
e-value: 2.4E-53
score: 183.1
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 18..257
NoneNo IPR availablePANTHERPTHR24320:SF198NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 18..257
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 65..251

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G001230.2CsGy1G001230.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity