CsGy1G000970 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G000970
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionE3 ubiquitin-protein ligase RGLG2-like
LocationGy14Chr1: 544741 .. 547129 (-)
RNA-Seq ExpressionCsGy1G000970
SyntenyCsGy1G000970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGGAAAAAGTTCTAAGCATAGAAGCTCAAGACGGAATTCGTCTAGTCGGTCGAACTCACAGCAGTGGAGTCATTATGGTTATCCGGAGTCGCCATATACTCAATCGAGAAGCACGCCGCAGTATGAGTATGCACCTCCAACTCCTAGCTATGGTGGAACTCAAGCCCCTGAGACAAGAAAGAGGCTGGAGAGGAAGTATTCAAGGATAGATGATAACTATAACAGTCTAGATCAGGTCTTGCTGCAATCATATTGCAAGACTTTATTGGCACTCCATAGTCAATATGAAACTTACTTCTACGCTCTGATACTCTTCATAGGTTACTGAAGCATTAGCTCGTGCTGGCCTGGAGTCCTCGAATCTTATCGTTGGTATAGATTTCACAAAGAGCAATGAGTGGACAGGTTCAGCCTTGCTTTGGCCATTAGATGTTCTTGTATCGGTTTCCTTAATAGCATGTGCACATGTTAACTATGGCCTTTGTATAGGTGCAAGATCATTCAACCGTAGAAGTTTGCATCATATTGGAGAAGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGCCAAACATTATCTTCCTTTGATGAGGATAATATGATTCCTTGCTTCGGTTTTGGAGATGGTATTTCTTCAAAAGAATTGTGTAATCGATCCATTTTTCGCTGACACTTGTTTGAATTAACAGTAGTGAAGTATGTGCATTTCAATGTTGGCAGCATCCACTCATGACCAAGAGGTTTTCAGCTTTTATTCCGATGAGCGATTTTGTAATGGATTTGAAGAAGTGTTGGGTAGGTACAAGGAACTAGTGCCGCATCTTCGACTCGCAGGTTGCAGTCTGCCTGTTTGCTATGTGATGTCATACTTCTCTATTGTTGTTATTTTTTACTTACTATACTTTCATTATTCATCCCTGGGACAGGACCTACATCTTTTGCACCTATCATTGAAATGGCTATCACAATTGTTGAGCAAAGCGGCGGCCAGTACCATGTATTGGTAATAATAGCCGATGGTCAGGTACTTTCATCTTCATTCCCACTGCACTTCAATTTTGCTTGATATAGAGGTTCATGTTTATAACATAACTTCCTGTTTGTTCCCAGATGTTCAAAAAGTTAGTAGTTAAGCAACGATGTATGTTGCCATTTCCATTCATAATCATTATCGTGGCTGATTAAATTGAGAGTTCAATTGGTTTTATTTATGAAGCAATACACTCAGCAAGCTGACTACTCTCTTCAGGTTACAAGAAGTGTTGATACTGAGCGTGGACAGTTTAGCCCACAGGAAAAGAAAACCATTGAAGCAATAGTGAAAGCTAGGTAGGTTCAAGGTATCTATAATGGACAGAAATATGACATTGTTCGTATTCCTACGCTGCGGAAGTCTGTAACTTTGTGCTGAATTGCAGCAAATATCCCCTGTCGATTATACTAGTTGGAGTAGGAGATGGGCCTTGGGACATGATGAGGGAATTTGACGACAATATCCCAGCTCGAGCCTTCGATAACTTCCAAGTAAGTACTTCTCTTGGGAGGTTTTGGTATTTTGTTTATCAAGTTGAATTTGCTTCTGTCACTGTTTGAAGTCTATACGCAATCTGAGATTTGCACTTTTGATTCGACAGTTTGTGAATTTCACAAACATAATGTTAAAGAATATAGATCGATCCAGAAAGGAAGCAGAATTTGCTCTTGCAGCACTTATGGAAATACCCTCCCAGTACAAAGCAACACTGGAGCTTGGTCTTTTGGGGTAATAACAAGATTCAAGAGTAGTATATTCAAGATTTTTTTTTTCAGATAAATGATGATGTATTTCCTTTCATTATTTCTTTCTCAAATTATATTTTCTTTCACTGCCTCTACCAGTGTCAGTAGAGGGAAGGCAATCGACCGGATTCCTCTTCCCCCTCCTTTTTATGGACCCAGCGTCCGAAAAACCTCAAACTCAAGCAGTTCTCGCCCAACGCCACCCTCATTTAGTGGAGGTGCTCCTGGCCGAACTGCACCTCCTCTAAGTTCAGTTTCCGATGCCCATGTAAATGCTTCTTTTCCCTTTTTAGATTTATACCATCTTAGCGACTAAGGTTGACCTTCTATTCTATTTAGTAAAATGGAAATGAAGATTTTTTTACACCCTCTGATGCAGGCTTGTCCAATTTGCATCTCCAATGCTAAGGATATGGCTTTTGGCTGTGGACATCAGGTAATCATTATCTTCCAAATTGAGTTTTTGATGCCTATCTGAAACATTGATCTGAAGAAACGCTAACCTTTTACTTATCTGCAGACATGTTGTGAATGTGGACAAGATCTTCAGCTGTGTCCCATATGCCGCAGCTTTATCGACACAAGGATAAAGCTATATTAG

mRNA sequence

ATGGGTGGAAAAAGTTCTAAGCATAGAAGCTCAAGACGGAATTCGTCTAGTCGGTCGAACTCACAGCAGTGGAGTCATTATGGTTATCCGGAGTCGCCATATACTCAATCGAGAAGCACGCCGCAGTATGAGTATGCACCTCCAACTCCTAGCTATGGTGGAACTCAAGCCCCTGAGACAAGAAAGAGGCTGGAGAGGAAGTATTCAAGGATAGATGATAACTATAACAGTCTAGATCAGGTTACTGAAGCATTAGCTCGTGCTGGCCTGGAGTCCTCGAATCTTATCGTTGGTATAGATTTCACAAAGAGCAATGAGTGGACAGGTGCAAGATCATTCAACCGTAGAAGTTTGCATCATATTGGAGAAGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGCCAAACATTATCTTCCTTTGATGAGGATAATATGATTCCTTGCTTCGGTTTTGGAGATGCATCCACTCATGACCAAGAGGTTTTCAGCTTTTATTCCGATGAGCGATTTTGTAATGGATTTGAAGAAGTGTTGGGTAGGTACAAGGAACTAGTGCCGCATCTTCGACTCGCAGGACCTACATCTTTTGCACCTATCATTGAAATGGCTATCACAATTGTTGAGCAAAGCGGCGGCCAGTACCATGTATTGGTAATAATAGCCGATGGTCAGGTTACAAGAAGTGTTGATACTGAGCGTGGACAGTTTAGCCCACAGGAAAAGAAAACCATTGAAGCAATAGTGAAAGCTAGCAAATATCCCCTGTCGATTATACTAGTTGGAGTAGGAGATGGGCCTTGGGACATGATGAGGGAATTTGACGACAATATCCCAGCTCGAGCCTTCGATAACTTCCAATTTGTGAATTTCACAAACATAATGTTAAAGAATATAGATCGATCCAGAAAGGAAGCAGAATTTGCTCTTGCAGCACTTATGGAAATACCCTCCCAGTACAAAGCAACACTGGAGCTTGGTCTTTTGGGTGTCAGTAGAGGGAAGGCAATCGACCGGATTCCTCTTCCCCCTCCTTTTTATGGACCCAGCGTCCGAAAAACCTCAAACTCAAGCAGTTCTCGCCCAACGCCACCCTCATTTAGTGGAGGTGCTCCTGGCCGAACTGCACCTCCTCTAAGTTCAGTTTCCGATGCCCATGCTTGTCCAATTTGCATCTCCAATGCTAAGGATATGGCTTTTGGCTGTGGACATCAGACATGTTGTGAATGTGGACAAGATCTTCAGCTGTGTCCCATATGCCGCAGCTTTATCGACACAAGGATAAAGCTATATTAG

Coding sequence (CDS)

ATGGGTGGAAAAAGTTCTAAGCATAGAAGCTCAAGACGGAATTCGTCTAGTCGGTCGAACTCACAGCAGTGGAGTCATTATGGTTATCCGGAGTCGCCATATACTCAATCGAGAAGCACGCCGCAGTATGAGTATGCACCTCCAACTCCTAGCTATGGTGGAACTCAAGCCCCTGAGACAAGAAAGAGGCTGGAGAGGAAGTATTCAAGGATAGATGATAACTATAACAGTCTAGATCAGGTTACTGAAGCATTAGCTCGTGCTGGCCTGGAGTCCTCGAATCTTATCGTTGGTATAGATTTCACAAAGAGCAATGAGTGGACAGGTGCAAGATCATTCAACCGTAGAAGTTTGCATCATATTGGAGAAGAGCAAAATCCCTATGAACAAGCAATAGCAATTATTGGCCAAACATTATCTTCCTTTGATGAGGATAATATGATTCCTTGCTTCGGTTTTGGAGATGCATCCACTCATGACCAAGAGGTTTTCAGCTTTTATTCCGATGAGCGATTTTGTAATGGATTTGAAGAAGTGTTGGGTAGGTACAAGGAACTAGTGCCGCATCTTCGACTCGCAGGACCTACATCTTTTGCACCTATCATTGAAATGGCTATCACAATTGTTGAGCAAAGCGGCGGCCAGTACCATGTATTGGTAATAATAGCCGATGGTCAGGTTACAAGAAGTGTTGATACTGAGCGTGGACAGTTTAGCCCACAGGAAAAGAAAACCATTGAAGCAATAGTGAAAGCTAGCAAATATCCCCTGTCGATTATACTAGTTGGAGTAGGAGATGGGCCTTGGGACATGATGAGGGAATTTGACGACAATATCCCAGCTCGAGCCTTCGATAACTTCCAATTTGTGAATTTCACAAACATAATGTTAAAGAATATAGATCGATCCAGAAAGGAAGCAGAATTTGCTCTTGCAGCACTTATGGAAATACCCTCCCAGTACAAAGCAACACTGGAGCTTGGTCTTTTGGGTGTCAGTAGAGGGAAGGCAATCGACCGGATTCCTCTTCCCCCTCCTTTTTATGGACCCAGCGTCCGAAAAACCTCAAACTCAAGCAGTTCTCGCCCAACGCCACCCTCATTTAGTGGAGGTGCTCCTGGCCGAACTGCACCTCCTCTAAGTTCAGTTTCCGATGCCCATGCTTGTCCAATTTGCATCTCCAATGCTAAGGATATGGCTTTTGGCTGTGGACATCAGACATGTTGTGAATGTGGACAAGATCTTCAGCTGTGTCCCATATGCCGCAGCTTTATCGACACAAGGATAAAGCTATATTAG

Protein sequence

MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSSSRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY*
Homology
BLAST of CsGy1G000970 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.3e-162
Identity = 306/458 (66.81%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 4   KSSKHRSSRRNSSSRSNSQQWSHYG-----YPESP----------------YTQSRSTPQ 63
           +S+   S+  +SSS ++ Q +  YG     YP  P                 TQ  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 64  YEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFT 123
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SL+QVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 124 KSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 183
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 184 VFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 243
           VFSF S++RFCNGFEEVL RYKE+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 244 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPAR 303
           ADGQVTRSVDTE GQ SPQE+KT++AIV+ASK PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 304 AFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIP 363
           AFDNFQFVNFT IM KN  +S KE EFAL+ALMEIP QYKAT+EL LLG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 364 LPPPFYGPSVRKTSNSSSSRPT-----PPSFSGGAPGRTA---PPLSSVSDAHACPICIS 423
           LPPP  G S    S   S  P+     PP  + G   R++   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 424 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of CsGy1G000970 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.2e-157
Identity = 285/412 (69.17%), Postives = 327/412 (79.37%), Query Frame = 0

Query: 25  SHYG--YPESPYTQSRSTPQYEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVT 84
           S YG  Y +  Y  + S P Y   PP PS         RK+ +R+YS+I DNY+SL QV+
Sbjct: 91  SSYGHQYSQEGYASAASQPNY---PPPPSQSQV---ADRKKFDRRYSKISDNYSSLLQVS 150

Query: 85  EALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSF 144
           EAL RAGLESSNLIVGIDFTKSNEWTGA+SFNR+SLHH+    NPYEQAI IIG+TL++F
Sbjct: 151 EALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAF 210

Query: 145 DEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPII 204
           DEDN+IPC+GFGDASTHDQ+VFSFY + RFCNGFEEVL RY+E+VP L+LAGPTSFAPII
Sbjct: 211 DEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPII 270

Query: 205 EMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILV 264
           EMA+T+VEQS GQYHVLVIIADGQVTRSVDTE G+ SPQE+KT++AIVKAS  PLSI+LV
Sbjct: 271 EMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLV 330

Query: 265 GVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYK 324
           GVGDGPWDMM+EFDDNIPARAFDNFQFVNFT IM KN D+SRKE EFAL+ALMEIP QYK
Sbjct: 331 GVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYK 390

Query: 325 ATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSSSRPTPPSFSGGAPGR--TAPPL 384
           AT+EL LLGV  G    RIPLPPP     V+  S+ SSSR   P+F    P     +  +
Sbjct: 391 ATIELNLLGVRNGNIPQRIPLPPP-----VQSGSSFSSSR--IPNFEPSVPPYPFESKQM 450

Query: 385 SSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           SS  D   CPIC+SN K+MAFGCGHQTCCECG DL++CPICR+ I TRIKLY
Sbjct: 451 SSADDIQLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKLY 489

BLAST of CsGy1G000970 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 8.5e-150
Identity = 286/441 (64.85%), Postives = 335/441 (75.96%), Query Frame = 0

Query: 1   MGGKSSKHR-----SSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGT 60
           MGG SSK       S RR   S S S  +S   + +S Y Q   TP +  A P  SY   
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSS-AWDQSSYYQ---TPNHPSASPVSSYNSG 60

Query: 61  QAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 120
           +  +T K LERKYSRI DNY S+D+VT AL+ AGLESSNLIVGID TKSNEWTGARSF R
Sbjct: 61  R--QTPKNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGR 120

Query: 121 RSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNG 180
           +SLH IG   NPY+QAI+IIG+TLS FDEDN+IPC+GFGDA+THDQ+VFSF  ++ +CNG
Sbjct: 121 KSLHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNG 180

Query: 181 FEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTER 240
           FEEVL  Y+E+VP LRL+GPTSFAPIIE A+TIVE+SGGQYHVL+IIADGQVTRSVDT+ 
Sbjct: 181 FEEVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDN 240

Query: 241 GQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNI 300
           G FSPQE++TI+AIV+AS+YPLSI+LVGVGDGPWD MR+FDDNIPARAFDNFQFVNFT+I
Sbjct: 241 GGFSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDI 300

Query: 301 MLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYG-PSVR- 360
           M KNID +RKEAEFAL+ALMEIPSQYKATLELGLLG   G   DRI LPPP Y   S+R 
Sbjct: 301 MSKNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPPTYATQSMRN 360

Query: 361 --KTSNSSSSRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCEC 420
             +TS S+S +  P  +  G          + S    CP+C+ +AK+MAF CGHQTC  C
Sbjct: 361 SPRTSRSTSFQNKP--YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAGC 420

Query: 421 GQDLQLCPICRSFIDTRIKLY 433
           G+DL +CPICRS I  RIKLY
Sbjct: 421 GEDLHVCPICRSSISVRIKLY 433

BLAST of CsGy1G000970 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.8e-120
Identity = 222/364 (60.99%), Postives = 268/364 (73.63%), Query Frame = 0

Query: 69  SRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGEEQNPY 128
           S I D+++SLDQV  +L  AGLESSNLI+GIDFTKSNEWTG  SFNR+SLH IG+ QNPY
Sbjct: 12  SYIADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAIGKRQNPY 71

Query: 129 EQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVLGRYKELVP 188
           E+AI+IIG+TLS FDED++IPCFGFGD +T DQ VFSFY + + C+G E  + RY+E+VP
Sbjct: 72  EKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVP 131

Query: 189 HLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEA 248
           HL+L+GPTSFAP+I+ AI IVEQ+  QYHVLVIIADGQVTR+ D   G+ SPQE+ T+ +
Sbjct: 132 HLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNS 191

Query: 249 IVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNIDRSRKEAE 308
           I+ AS YPLSI+LVGVGDGPWD M++FDDNIP R FDNFQFVNFT IM ++ D ++KEA 
Sbjct: 192 IMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDAAKKEAA 251

Query: 309 FALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSSSRPTPPSF 368
           FALAALMEIP QYKATL L    V R       PLPPP   P V +  N+  S P   + 
Sbjct: 252 FALAALMEIPFQYKATLSLNRKPV-RSSHQHHKPLPPP---PEVIERDNAVRSVPNQMTE 311

Query: 369 SGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTR 428
           +     R AP     S    CPIC++N KDMAF CGH TC ECG  +  CP+CR  I TR
Sbjct: 312 TAEKSDRLAP-----STVPVCPICLTNPKDMAFSCGHTTCKECGVVITTCPLCRQPITTR 366

Query: 429 IKLY 433
           I+LY
Sbjct: 372 IRLY 366

BLAST of CsGy1G000970 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 7.0e-120
Identity = 218/405 (53.83%), Postives = 273/405 (67.41%), Query Frame = 0

Query: 28  GYPESPYTQSRSTPQYEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALAR 87
           G   S    +  T   +   P+PS       +     ++KY+ I D ++SLDQV++AL  
Sbjct: 13  GKSRSSRNMTLGTTSSQSHEPSPSDPSLSLADNTNATKKKYALIPDRFSSLDQVSKALRE 72

Query: 88  AGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNM 147
           AGLESSNLI+G+DFTKSNEWTG  SF+ + LH +GE  NPYE+AI +IGQTL+ FDEDN+
Sbjct: 73  AGLESSNLILGVDFTKSNEWTGKTSFDGKCLHALGETSNPYEKAIFVIGQTLAPFDEDNL 132

Query: 148 IPCFGFGDASTHDQEVFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAIT 207
           IPCFGFGD++THD+EVF F+SD   C+GFEEVL  YK + P+LRL+GPTS+ P+I+ A+ 
Sbjct: 133 IPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKRIAPNLRLSGPTSYGPLIDAAVD 192

Query: 208 IVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDG 267
           IVE++ GQ+HVLVI+ADGQVTR  D   G+ S QEK TI+AIV AS Y LSI+LVGVGDG
Sbjct: 193 IVEKNNGQFHVLVIVADGQVTRGTDMAEGELSQQEKTTIDAIVNASSYALSIVLVGVGDG 252

Query: 268 PWDMMREFDDNIPARAFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLEL 327
           PW+ MR+FDD IP R FDNFQFVNFT IM +N   S KE  FALAALMEIP QY+A +EL
Sbjct: 253 PWEDMRKFDDKIPKREFDNFQFVNFTEIMTRNSPESAKETAFALAALMEIPFQYQAAIEL 312

Query: 328 GLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSSSRPTPPSFSGGAPGRTAPPLSSVSDAH 387
            LLG   G A   +P PPP                P  P  +   P   +P  +S     
Sbjct: 313 RLLGKQTGLAKTIVPRPPPI---------------PYTPPTNAELPSTASP--ASPEQTQ 372

Query: 388 ACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           +CPIC++N KD+AF CGH TC +CG  +  CPICR  I  R+KLY
Sbjct: 373 SCPICLTNRKDVAFSCGHMTCGDCGSKISNCPICRVRITNRLKLY 400

BLAST of CsGy1G000970 vs. NCBI nr
Match: XP_004138080.1 (E3 ubiquitin-protein ligase RGLG2 [Cucumis sativus])

HSP 1 Score: 865 bits (2234), Expect = 0.0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET
Sbjct: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS
Sbjct: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 432

BLAST of CsGy1G000970 vs. NCBI nr
Match: XP_008464499.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_008464500.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_008464501.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_016903213.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_016903214.1 PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >KAA0057810.1 E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo var. makuwa] >TYJ98494.1 E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 839 bits (2168), Expect = 1.36e-306
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 1   MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 60

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 121 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 360

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 430

BLAST of CsGy1G000970 vs. NCBI nr
Match: XP_016903211.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo])

HSP 1 Score: 839 bits (2168), Expect = 3.14e-306
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 23  MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 82

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 83  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 142

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 143 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 202

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 203 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 262

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 263 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 322

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 323 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 382

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 383 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 442

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 443 CRSFIDTRIKLY 452

BLAST of CsGy1G000970 vs. NCBI nr
Match: XP_016903210.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X2 [Cucumis melo])

HSP 1 Score: 839 bits (2168), Expect = 4.58e-306
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 33  MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 92

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 93  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 152

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 153 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 212

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 213 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 272

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 273 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 332

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 333 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 392

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 393 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 452

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 453 CRSFIDTRIKLY 462

BLAST of CsGy1G000970 vs. NCBI nr
Match: XP_016903209.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Cucumis melo])

HSP 1 Score: 839 bits (2168), Expect = 5.33e-306
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 37  MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 96

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 97  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 156

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 157 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 216

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 217 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 276

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 277 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 336

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 337 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 396

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 397 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 456

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 457 CRSFIDTRIKLY 466

BLAST of CsGy1G000970 vs. ExPASy TrEMBL
Match: A0A0A0LP13 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G002920 PE=4 SV=1)

HSP 1 Score: 865 bits (2234), Expect = 0.0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET
Sbjct: 44  MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 103

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 104 RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 163

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 164 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 223

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 224 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 283

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 284 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 343

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS
Sbjct: 344 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 403

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 404 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 463

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 464 CRSFIDTRIKLY 475

BLAST of CsGy1G000970 vs. ExPASy TrEMBL
Match: A0A1S3CLK7 (E3 ubiquitin-protein ligase RGLG2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 6.60e-307
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 1   MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 60

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 121 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 360

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 430

BLAST of CsGy1G000970 vs. ExPASy TrEMBL
Match: A0A5A7URQ8 (E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00960 PE=4 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 6.60e-307
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 1   MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 60

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 121 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 360

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 361 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 421 CRSFIDTRIKLY 430

BLAST of CsGy1G000970 vs. ExPASy TrEMBL
Match: A0A1S4E4Q2 (E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 1.52e-306
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 23  MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 82

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 83  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 142

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 143 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 202

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 203 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 262

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 263 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 322

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 323 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 382

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 383 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 442

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 443 CRSFIDTRIKLY 452

BLAST of CsGy1G000970 vs. ExPASy TrEMBL
Match: A0A1S4E5G0 (E3 ubiquitin-protein ligase RGLG2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502363 PE=4 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 2.22e-306
Identity = 422/432 (97.69%), Postives = 426/432 (98.61%), Query Frame = 0

Query: 1   MGGKSSKHRSSRRNSSSRSNSQQWSHYGYPESPYTQSRSTPQYEYAPPTPSYGGTQAPET 60
           MGGKSSKH SSRRNSSSRSNSQQW HYGYPESPYTQSRSTP+Y  APPTPSYGGTQAPET
Sbjct: 33  MGGKSSKHGSSRRNSSSRSNSQQWGHYGYPESPYTQSRSTPRY--APPTPSYGGTQAPET 92

Query: 61  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 120
           RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH
Sbjct: 93  RKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHH 152

Query: 121 IGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 180
           IG+EQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL
Sbjct: 153 IGDEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVL 212

Query: 181 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 240
           GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP
Sbjct: 213 GRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSP 272

Query: 241 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 300
           QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI
Sbjct: 273 QEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNI 332

Query: 301 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSS 360
           DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPS++KTSNSSS
Sbjct: 333 DRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIPLPPPFYGPSIQKTSNSSS 392

Query: 361 SRPTPPSFSGGAPGRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 420
           SRPTPPSFSG AP RTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI
Sbjct: 393 SRPTPPSFSGVAPVRTAPPLSSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPI 452

Query: 421 CRSFIDTRIKLY 432
           CRSFIDTRIKLY
Sbjct: 453 CRSFIDTRIKLY 462

BLAST of CsGy1G000970 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 306/458 (66.81%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 4   KSSKHRSSRRNSSSRSNSQQWSHYG-----YPESP----------------YTQSRSTPQ 63
           +S+   S+  +SSS ++ Q +  YG     YP  P                 TQ  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 64  YEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFT 123
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SL+QVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 124 KSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 183
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 184 VFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 243
           VFSF S++RFCNGFEEVL RYKE+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 244 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPAR 303
           ADGQVTRSVDTE GQ SPQE+KT++AIV+ASK PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 304 AFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIP 363
           AFDNFQFVNFT IM KN  +S KE EFAL+ALMEIP QYKAT+EL LLG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 364 LPPPFYGPSVRKTSNSSSSRPT-----PPSFSGGAPGRTA---PPLSSVSDAHACPICIS 423
           LPPP  G S    S   S  P+     PP  + G   R++   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 424 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of CsGy1G000970 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 306/458 (66.81%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 4   KSSKHRSSRRNSSSRSNSQQWSHYG-----YPESP----------------YTQSRSTPQ 63
           +S+   S+  +SSS ++ Q +  YG     YP  P                 TQ  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 64  YEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFT 123
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SL+QVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 124 KSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 183
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 184 VFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 243
           VFSF S++RFCNGFEEVL RYKE+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 244 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPAR 303
           ADGQVTRSVDTE GQ SPQE+KT++AIV+ASK PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 304 AFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIP 363
           AFDNFQFVNFT IM KN  +S KE EFAL+ALMEIP QYKAT+EL LLG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 364 LPPPFYGPSVRKTSNSSSSRPT-----PPSFSGGAPGRTA---PPLSSVSDAHACPICIS 423
           LPPP  G S    S   S  P+     PP  + G   R++   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 424 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of CsGy1G000970 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 306/458 (66.81%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 4   KSSKHRSSRRNSSSRSNSQQWSHYG-----YPESP----------------YTQSRSTPQ 63
           +S+   S+  +SSS ++ Q +  YG     YP  P                 TQ  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 64  YEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFT 123
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SL+QVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 124 KSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 183
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 184 VFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 243
           VFSF S++RFCNGFEEVL RYKE+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 244 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPAR 303
           ADGQVTRSVDTE GQ SPQE+KT++AIV+ASK PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 304 AFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIP 363
           AFDNFQFVNFT IM KN  +S KE EFAL+ALMEIP QYKAT+EL LLG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 364 LPPPFYGPSVRKTSNSSSSRPT-----PPSFSGGAPGRTA---PPLSSVSDAHACPICIS 423
           LPPP  G S    S   S  P+     PP  + G   R++   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 424 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of CsGy1G000970 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 572.8 bits (1475), Expect = 2.4e-163
Identity = 306/458 (66.81%), Postives = 349/458 (76.20%), Query Frame = 0

Query: 4   KSSKHRSSRRNSSSRSNSQQWSHYG-----YPESP----------------YTQSRSTPQ 63
           +S+   S+  +SSS ++ Q +  YG     YP  P                 TQ  S P 
Sbjct: 16  RSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQPPPPLYSTQPYSAPS 75

Query: 64  YEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVTEALARAGLESSNLIVGIDFT 123
           Y  APP+ SYG     + +KRLERKYS+I D+Y+SL+QVTEALARAGLESSNLIVGIDFT
Sbjct: 76  YS-APPSQSYGS----DNKKRLERKYSKISDDYSSLEQVTEALARAGLESSNLIVGIDFT 135

Query: 124 KSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSFDEDNMIPCFGFGDASTHDQE 183
           KSNEWTGARSFNR+SLH IG   NPYEQAI IIG+TL++FDEDN+IPC+GFGDASTHDQ+
Sbjct: 136 KSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQD 195

Query: 184 VFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPIIEMAITIVEQSGGQYHVLVII 243
           VFSF S++RFCNGFEEVL RYKE+VP L+LAGPTSFAPII+MA+TIVEQSGGQYHVLVII
Sbjct: 196 VFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVII 255

Query: 244 ADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILVGVGDGPWDMMREFDDNIPAR 303
           ADGQVTRSVDTE GQ SPQE+KT++AIV+ASK PLSI+LVGVGDGPWDMMREFDDNIPAR
Sbjct: 256 ADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPAR 315

Query: 304 AFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYKATLELGLLGVSRGKAIDRIP 363
           AFDNFQFVNFT IM KN  +S KE EFAL+ALMEIP QYKAT+EL LLG   G   +R P
Sbjct: 316 AFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGRRNGYIPERFP 375

Query: 364 LPPPFYGPSVRKTSNSSSSRPT-----PPSFSGGAPGRTA---PPLSSVSDAHACPICIS 423
           LPPP  G S    S   S  P+     PP  + G   R++   PP SS SD   CPIC+S
Sbjct: 376 LPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSASDNQLCPICLS 435

Query: 424 NAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           N KDMAFGCGHQTCCECG DLQ+CPICR+ I TRIKLY
Sbjct: 436 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of CsGy1G000970 vs. TAIR 10
Match: AT3G01650.1 (RING domain ligase1 )

HSP 1 Score: 556.2 bits (1432), Expect = 2.3e-158
Identity = 285/412 (69.17%), Postives = 327/412 (79.37%), Query Frame = 0

Query: 25  SHYG--YPESPYTQSRSTPQYEYAPPTPSYGGTQAPETRKRLERKYSRIDDNYNSLDQVT 84
           S YG  Y +  Y  + S P Y   PP PS         RK+ +R+YS+I DNY+SL QV+
Sbjct: 91  SSYGHQYSQEGYASAASQPNY---PPPPSQSQV---ADRKKFDRRYSKISDNYSSLLQVS 150

Query: 85  EALARAGLESSNLIVGIDFTKSNEWTGARSFNRRSLHHIGEEQNPYEQAIAIIGQTLSSF 144
           EAL RAGLESSNLIVGIDFTKSNEWTGA+SFNR+SLHH+    NPYEQAI IIG+TL++F
Sbjct: 151 EALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAF 210

Query: 145 DEDNMIPCFGFGDASTHDQEVFSFYSDERFCNGFEEVLGRYKELVPHLRLAGPTSFAPII 204
           DEDN+IPC+GFGDASTHDQ+VFSFY + RFCNGFEEVL RY+E+VP L+LAGPTSFAPII
Sbjct: 211 DEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPII 270

Query: 205 EMAITIVEQSGGQYHVLVIIADGQVTRSVDTERGQFSPQEKKTIEAIVKASKYPLSIILV 264
           EMA+T+VEQS GQYHVLVIIADGQVTRSVDTE G+ SPQE+KT++AIVKAS  PLSI+LV
Sbjct: 271 EMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLV 330

Query: 265 GVGDGPWDMMREFDDNIPARAFDNFQFVNFTNIMLKNIDRSRKEAEFALAALMEIPSQYK 324
           GVGDGPWDMM+EFDDNIPARAFDNFQFVNFT IM KN D+SRKE EFAL+ALMEIP QYK
Sbjct: 331 GVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYK 390

Query: 325 ATLELGLLGVSRGKAIDRIPLPPPFYGPSVRKTSNSSSSRPTPPSFSGGAPGR--TAPPL 384
           AT+EL LLGV  G    RIPLPPP     V+  S+ SSSR   P+F    P     +  +
Sbjct: 391 ATIELNLLGVRNGNIPQRIPLPPP-----VQSGSSFSSSR--IPNFEPSVPPYPFESKQM 450

Query: 385 SSVSDAHACPICISNAKDMAFGCGHQTCCECGQDLQLCPICRSFIDTRIKLY 433
           SS  D   CPIC+SN K+MAFGCGHQTCCECG DL++CPICR+ I TRIKLY
Sbjct: 451 SSADDIQLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKLY 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LY873.3e-16266.81E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 ... [more]
Q9SS903.2e-15769.17E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 ... [more]
Q8LB888.5e-15064.85E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 ... [more]
Q8RX261.8e-12060.99E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 ... [more]
Q9SAL07.0e-12053.83E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_004138080.10.0100.00E3 ubiquitin-protein ligase RGLG2 [Cucumis sativus][more]
XP_008464499.11.36e-30697.69PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X4 [Cucumis melo] >XP_... [more]
XP_016903211.13.14e-30697.69PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X3 [Cucumis melo][more]
XP_016903210.14.58e-30697.69PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X2 [Cucumis melo][more]
XP_016903209.15.33e-30697.69PREDICTED: E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0LP130.0100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G002920 P... [more]
A0A1S3CLK76.60e-30797.69E3 ubiquitin-protein ligase RGLG2-like isoform X4 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5A7URQ86.60e-30797.69E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo var. makuwa OX... [more]
A0A1S4E4Q21.52e-30697.69E3 ubiquitin-protein ligase RGLG2-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S4E5G02.22e-30697.69E3 ubiquitin-protein ligase RGLG2-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G14420.12.4e-16366.81RING domain ligase2 [more]
AT5G14420.32.4e-16366.81RING domain ligase2 [more]
AT5G14420.42.4e-16366.81RING domain ligase2 [more]
AT5G14420.22.4e-16366.81RING domain ligase2 [more]
AT3G01650.12.3e-15869.17RING domain ligase1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 61..81
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 387..426
e-value: 1.0E-6
score: 28.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..61
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 348..379
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..367
NoneNo IPR availablePANTHERPTHR45751:SF29RING-FINGER PROTEINcoord: 1..432
NoneNo IPR availablePANTHERPTHR45751COPINE FAMILY PROTEIN 1coord: 1..432
NoneNo IPR availableCDDcd16729RING-HC_RGLG_plantcoord: 389..432
e-value: 1.04892E-24
score: 94.0427
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 381..427
IPR002035von Willebrand factor, type ASMARTSM00327VWA_4coord: 92..295
e-value: 1.3E-8
score: 44.6
IPR010734Copine, C-terminalPFAMPF07002Copinecoord: 117..330
e-value: 2.0E-75
score: 253.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 376..432
e-value: 2.4E-10
score: 41.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 389..422
score: 10.792683
IPR036465von Willebrand factor A-like domain superfamilySUPERFAMILY53300vWA-likecoord: 93..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G000970.2CsGy1G000970.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity