CsGy1G000060 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy1G000060
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionMagnesium protoporphyrin IX methyltransferase
LocationGy14Chr1: 44373 .. 45963 (-)
RNA-Seq ExpressionCsGy1G000060
SyntenyCsGy1G000060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATTTGTTTTAAGTGTACTTTTTTTCGAGTTACAAAGTTAAATGGGTAATTTGCATAATTTAGCCCATATATTATTTTTTTTTCTTTGGCAAGGATGAGCCACCGTGAGCATGGGACCCAGGGGAATCGGTGAAAGATAATCCACAAAGAGGTGTGTGGATGAGCTTGGGGTTGCGCCTTAGCCACTCCTCATCACAGTTTAGACGCAGCCTCACATACTTCTCCCCTGCCACCGCCGCTCGGAAGGAGCAGTTATTCGGTAACCATGGCTCACTCTGCATTATCTCGATATCCCACACTTTGCTTGCACCCCGATTCTACCTCAAATTCTAGATTCTTCTTATCCCCAACACCTATCAAGCTCATTAAATTTTCAAGAACGCTCAATCTTAATGTCTCTGCTGTAGCTACCGAACTTCCCATCGTCGACGGAGCAACAATTGCAGCCATCGGTGGCGGTTCGGTTGCCGCGCTGGCTGCCGTTCTATCTCTGACCGATCCGGAGAGACGCCGCCGCCTGCAGGCCGAAGAGGTGGGTGGTGGCGACAAGGATGTGGTGAAGGAATACTTTAACAATTCGGGGTTTCAGAGGTGGAAGAAAATTTATGGAGAGACGGATGATGTGAACCGGGTGCAACGAGACATTAGGTTGGGTCACTCCAAGACGGTGGAGAATGTGATGGCGATGTTGAAAGACGAGGGCTCACTGAGAGGAGTAACAGTGTGCGATGCCGGCTGTGGAACCGGTAGCTTATCTATTCCGCTAGCCAAGGAAGGCGCGATGGTCTTCGCTAGCGATATTTCTGCTGCTATGGTCGCGGAGGCCGAAAACAAGGTGCGCACTTCCTTTCTCTCAGTCATACTCATAATACTAATGTACTGATTTGATTGAATTGTATATGATGTGGATCCAATTATTGGGTATGTGATTCCAGGCAAAAGAAGAGGTTGTGGGAGGCGTGGTGCCAAAATTTGAAGTGAAAGATTTGGAGAGCTTGGAAGGGAAATATGATACGGTGGTATGTCTAGATGTTTTAATACACTACCCCCAGAACAAGGCAGACGGAATGATTGCGCACCTTGCATCCCTAGCGGAAAAGCGTTTAATCATTAGCTTTGCGCCAAAAACATTTTACTACGATTTGTTGAAGAGGGTGGGAGAGTTGTTCCCCGGACCTTCAAAGGCAACTAGGGCATATCTGCACTCTGAGGCCGACGTCGAGACTGCATTGCGGAGGGCTGGCTGGAAAATAAGGAAGAGAGGTCTAATCTCTACCCAGTTTTACTTTGCCACATTGGTTGAGGCAGTTCCTGCTTGACACTTCACACCTGTCTTTCGTTGCGTTTCATTTCACTTTTGACACTTCCTCTACCCGCCCTCCTTCTGTATTCCAGTGTTTTTACTTACAATGCTTCCTAAGTAGAGTGTCAACATTATCTTATGTATGCTAATGGATTCCACTTTATATTAATCTAAACTCCGATGCTTAAGTTCTCTTTTTTAAGAAAGAGGATTACGAGCCAAAACATCCCTTCGAGGAGGTTGGACCTGGACCTAGTGGGAAACATTCTACCATTTTTCATTTCAAT

mRNA sequence

ACATTTGTTTTAAGTGTACTTTTTTTCGAGTTACAAAGTTAAATGGGTAATTTGCATAATTTAGCCCATATATTATTTTTTTTTCTTTGGCAAGGATGAGCCACCGTGAGCATGGGACCCAGGGGAATCGGTGAAAGATAATCCACAAAGAGGTGTGTGGATGAGCTTGGGGTTGCGCCTTAGCCACTCCTCATCACAGTTTAGACGCAGCCTCACATACTTCTCCCCTGCCACCGCCGCTCGGAAGGAGCAGTTATTCGCTACCGAACTTCCCATCGTCGACGGAGCAACAATTGCAGCCATCGGTGGCGGTTCGGTTGCCGCGCTGGCTGCCGTTCTATCTCTGACCGATCCGGAGAGACGCCGCCGCCTGCAGGCCGAAGAGGTGGGTGGTGGCGACAAGGATGTGGTGAAGGAATACTTTAACAATTCGGGGTTTCAGAGGTGGAAGAAAATTTATGGAGAGACGGATGATGTGAACCGGGTGCAACGAGACATTAGGTTGGGTCACTCCAAGACGGTGGAGAATGTGATGGCGATGTTGAAAGACGAGGGCTCACTGAGAGGAGTAACAGTGTGCGATGCCGGCTGTGGAACCGGTAGCTTATCTATTCCGCTAGCCAAGGAAGGCGCGATGGTCTTCGCTAGCGATATTTCTGCTGCTATGGTCGCGGAGGCCGAAAACAAGGCAAAAGAAGAGGTTGTGGGAGGCGTGGTGCCAAAATTTGAAGTGAAAGATTTGGAGAGCTTGGAAGGGAAATATGATACGGTGGTATGTCTAGATGTTTTAATACACTACCCCCAGAACAAGGCAGACGGAATGATTGCGCACCTTGCATCCCTAGCGGAAAAGCGTTTAATCATTAGCTTTGCGCCAAAAACATTTTACTACGATTTGTTGAAGAGGGTGGGAGAGTTGTTCCCCGGACCTTCAAAGGCAACTAGGGCATATCTGCACTCTGAGGCCGACGTCGAGACTGCATTGCGGAGGGCTGGCTGGAAAATAAGGAAGAGAGGTCTAATCTCTACCCAGTTTTACTTTGCCACATTGGTTGAGGCAGTTCCTGCTTGACACTTCACACCTGTCTTTCGTTGCGTTTCATTTCACTTTTGACACTTCCTCTACCCGCCCTCCTTCTGTATTCCAGTGTTTTTACTTACAATGCTTCCTAAGTAGAGTGTCAACATTATCTTATGTATGCTAATGGATTCCACTTTATATTAATCTAAACTCCGATGCTTAAGTTCTCTTTTTTAAGAAAGAGGATTACGAGCCAAAACATCCCTTCGAGGAGGTTGGACCTGGACCTAGTGGGAAACATTCTACCATTTTTCATTTCAAT

Coding sequence (CDS)

ATGAGCTTGGGGTTGCGCCTTAGCCACTCCTCATCACAGTTTAGACGCAGCCTCACATACTTCTCCCCTGCCACCGCCGCTCGGAAGGAGCAGTTATTCGCTACCGAACTTCCCATCGTCGACGGAGCAACAATTGCAGCCATCGGTGGCGGTTCGGTTGCCGCGCTGGCTGCCGTTCTATCTCTGACCGATCCGGAGAGACGCCGCCGCCTGCAGGCCGAAGAGGTGGGTGGTGGCGACAAGGATGTGGTGAAGGAATACTTTAACAATTCGGGGTTTCAGAGGTGGAAGAAAATTTATGGAGAGACGGATGATGTGAACCGGGTGCAACGAGACATTAGGTTGGGTCACTCCAAGACGGTGGAGAATGTGATGGCGATGTTGAAAGACGAGGGCTCACTGAGAGGAGTAACAGTGTGCGATGCCGGCTGTGGAACCGGTAGCTTATCTATTCCGCTAGCCAAGGAAGGCGCGATGGTCTTCGCTAGCGATATTTCTGCTGCTATGGTCGCGGAGGCCGAAAACAAGGCAAAAGAAGAGGTTGTGGGAGGCGTGGTGCCAAAATTTGAAGTGAAAGATTTGGAGAGCTTGGAAGGGAAATATGATACGGTGGTATGTCTAGATGTTTTAATACACTACCCCCAGAACAAGGCAGACGGAATGATTGCGCACCTTGCATCCCTAGCGGAAAAGCGTTTAATCATTAGCTTTGCGCCAAAAACATTTTACTACGATTTGTTGAAGAGGGTGGGAGAGTTGTTCCCCGGACCTTCAAAGGCAACTAGGGCATATCTGCACTCTGAGGCCGACGTCGAGACTGCATTGCGGAGGGCTGGCTGGAAAATAAGGAAGAGAGGTCTAATCTCTACCCAGTTTTACTTTGCCACATTGGTTGAGGCAGTTCCTGCTTGA

Protein sequence

MSLGLRLSHSSSQFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEAVPA*
Homology
BLAST of CsGy1G000060 vs. ExPASy Swiss-Prot
Match: Q9SW18 (Magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHLM PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.4e-119
Identity = 224/305 (73.44%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 7   LSHSSSQFRRSLTYFSPAT---------AARKEQLFATELPIVDGATIAAIGGGSVAALA 66
           L  SSS   + L  F  AT         AA       T+L  VD  TIA +GGGSVAALA
Sbjct: 7   LLSSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALA 66

Query: 67  AVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGH 126
           A++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH
Sbjct: 67  AMVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGH 126

Query: 127 SKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKA 186
           +KTVEN M ML ++ SL GVTVCDAGCGTG LSIPLAKEGA+V ASDISAAMVAEAE KA
Sbjct: 127 AKTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKA 186

Query: 187 KEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISF 246
           K ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SF
Sbjct: 187 KAQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSF 246

Query: 247 APKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATL 303
           APKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL + GWKI KRGL +TQFYF+ L
Sbjct: 247 APKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRL 306

BLAST of CsGy1G000060 vs. ExPASy Swiss-Prot
Match: Q55467 (Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=chlM PE=1 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 4.0e-69
Identity = 127/222 (57.21%), Postives = 169/222 (76.13%), Query Frame = 0

Query: 80  DKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTV 139
           DK +V++YFN++GF RW++IYG+   VN VQ+DIR+GH +TV++V+A L  +G+L G+ V
Sbjct: 8   DKTIVRDYFNSTGFDRWRRIYGD-GQVNFVQKDIRVGHQQTVDSVVAWLVADGNLPGLLV 67

Query: 140 CDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEG 199
           CDAGCG GSLSIPLA+ GA+V+ SDIS  MV EA+ KA+E +  G  P F  +DL  L G
Sbjct: 68  CDAGCGVGSLSIPLAQAGALVYGSDISEKMVGEAQQKAQEVLAYGNQPTFMTQDLAQLGG 127

Query: 200 KYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSK 259
           KYDTV+CLDVLIHYP  +A  MI+HLASLA++RLI+SFAPKT    +LK++G LFPGPSK
Sbjct: 128 KYDTVICLDVLIHYPTEEASAMISHLASLADRRLILSFAPKTLGLTVLKKIGGLFPGPSK 187

Query: 260 ATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEAV 302
            TRAY H EAD+   L   G+ I + G+ ST+FY++ ++EAV
Sbjct: 188 TTRAYQHKEADIRKILGDNGFSIARTGMTSTRFYYSRILEAV 228

BLAST of CsGy1G000060 vs. ExPASy Swiss-Prot
Match: P26236 (Magnesium-protoporphyrin O-methyltransferase OS=Rhodobacter capsulatus OX=1061 GN=bchM PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 9.3e-18
Identity = 62/220 (28.18%), Postives = 112/220 (50.91%), Query Frame = 0

Query: 84  VKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAG 143
           V+ YF+ +  + W ++    + V++V++ +R G       +++ L D+  L G  V DAG
Sbjct: 12  VEHYFDRTATRAWARLTTADEKVSKVRQTVREGRDTMRAVMLSRLPDD--LTGCRVMDAG 71

Query: 144 CGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDL-ESLEGKYD 203
           CGTG  ++ LA+ GA V A DIS  ++  A+++   E+ G V   F V D+ +   G++D
Sbjct: 72  CGTGLTTVELARRGADVVAVDISPQLIDIAKDRLPPELRGKV--SFHVGDMADPALGQFD 131

Query: 204 TVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATR 263
            VV +D LI+Y       ++  L       ++ + APKT +      +G+LFP  +++  
Sbjct: 132 YVVAMDSLIYYRAPDIGRVLTELGKRTHSAIVFTVAPKTAFLMAFWWLGKLFPRSNRSPV 191

Query: 264 AYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEAVP 303
              H+   ++   R AG  + K   ++  FY +  +E  P
Sbjct: 192 MIPHA---LDKLQRHAGDSLIKIDRVARGFYISECLEYRP 224

BLAST of CsGy1G000060 vs. ExPASy Swiss-Prot
Match: A7MPA9 (Ubiquinone biosynthesis O-methyltransferase OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=ubiG PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 5.3e-05
Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = 0

Query: 95  RWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLA 154
           RW    GE   ++R+   +RLG+         + +  G L G  V D GCG G LS  +A
Sbjct: 26  RWWDTEGEFKPLHRI-NPLRLGY---------IAERAGGLFGKKVLDVGCGGGILSESMA 85

Query: 155 KEGAMVFASDISAAMVAEAENKAKEEVVG-GVVPKFEVKDLESLEGKYDTVVCLDVLIHY 214
           +EGA V   D+ A  +A A   A E  V    V +   +      G YD V C+++L H 
Sbjct: 86  REGANVTGLDMGAEPLAVARLHALESGVELNYVQQTVEEHAAQHAGAYDVVTCMEMLEHV 140

Query: 215 PQNKA 219
           P  ++
Sbjct: 146 PDPRS 140

BLAST of CsGy1G000060 vs. ExPASy Swiss-Prot
Match: Q2NSL7 (Ubiquinone biosynthesis O-methyltransferase OS=Sodalis glossinidius (strain morsitans) OX=343509 GN=ubiG PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 5.3e-05
Identity = 39/121 (32.23%), Postives = 59/121 (48.76%), Query Frame = 0

Query: 95  RWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLA 154
           RW  + GE   ++ +   +RL +         +L+  G L G  V D GCG G L+  +A
Sbjct: 34  RWWDLEGEFKPLHHI-NPLRLDY---------ILERSGGLFGKNVLDVGCGGGILAESMA 93

Query: 155 KEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKD-LESLEGKYDTVVCLDVLIHY 214
           +EGA V   D+ A  +A A   A E  V     +  V++  E+  G YD V C+++L H 
Sbjct: 94  REGAKVTGLDMGAEPLAVARLHALESGVMLAYHQQTVEEHAEAHPGAYDVVTCMEMLEHV 144

BLAST of CsGy1G000060 vs. NCBI nr
Match: KGN63436.2 (hypothetical protein Csa_013123 [Cucumis sativus])

HSP 1 Score: 587 bits (1513), Expect = 4.34e-211
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MSLGLRLSHSSSQFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVL 60
           MSLGLRLSHSSSQFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVL
Sbjct: 1   MSLGLRLSHSSSQFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVL 60

Query: 61  SLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKT 120
           SLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKT
Sbjct: 61  SLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKT 120

Query: 121 VENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEE 180
           VENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEE
Sbjct: 121 VENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEE 180

Query: 181 VVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPK 240
           VVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPK
Sbjct: 181 VVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPK 240

Query: 241 TFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEA 300
           TFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEA
Sbjct: 241 TFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEA 300

Query: 301 VPA 303
           VPA
Sbjct: 301 VPA 303

BLAST of CsGy1G000060 vs. NCBI nr
Match: XP_004138019.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucumis sativus])

HSP 1 Score: 526 bits (1356), Expect = 6.12e-187
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 34  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 93
           ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF
Sbjct: 48  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 107

Query: 94  QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 153
           QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL
Sbjct: 108 QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 167

Query: 154 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 213
           AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY
Sbjct: 168 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 227

Query: 214 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 273
           PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET
Sbjct: 228 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 287

Query: 274 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           ALRRAGWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 288 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 317

BLAST of CsGy1G000060 vs. NCBI nr
Match: XP_008464364.1 (PREDICTED: magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucumis melo] >ADN33868.1 magnesium-protoporphyrin O-methyltransferase [Cucumis melo subsp. melo] >KAA0057727.1 magnesium protoporphyrin IX methyltransferase [Cucumis melo var. makuwa] >TYJ98412.1 magnesium protoporphyrin IX methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 517 bits (1332), Expect = 2.67e-183
Identity = 265/270 (98.15%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 34  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 93
           ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF
Sbjct: 47  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 106

Query: 94  QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 153
           QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG LRGVTVCDAGCGTGSLSIPL
Sbjct: 107 QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPL 166

Query: 154 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 213
           AKEGA VFASDISAAMVAEAENKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHY
Sbjct: 167 AKEGAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHY 226

Query: 214 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 273
           PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET
Sbjct: 227 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 286

Query: 274 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           ALRR GWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 287 ALRRVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of CsGy1G000060 vs. NCBI nr
Match: XP_022987155.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 516 bits (1328), Expect = 1.40e-182
Identity = 267/291 (91.75%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 13  QFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ 72
           +F R+    +PA A     + ATELP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ
Sbjct: 34  KFSRTPNVNAPAPAPPLSAV-ATELPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ 93

Query: 73  AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG 132
           AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEG
Sbjct: 94  AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEG 153

Query: 133 SLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVK 192
            L GVTVCDAGCGTGSLSIPLAKEGA+VFASDISAAMVAEA+NKAKEEV GGVVPKFEVK
Sbjct: 154 PLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVK 213

Query: 193 DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE 252
           DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE
Sbjct: 214 DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE 273

Query: 253 LFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           LFPGPSKATRAYLHSEADVETALRR GWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 274 LFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLISTQFYFATLVEAVPA 323

BLAST of CsGy1G000060 vs. NCBI nr
Match: XP_023516229.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6589339.1 Magnesium protoporphyrin IX methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023025.1 Magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 515 bits (1327), Expect = 1.99e-182
Identity = 266/291 (91.41%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 13  QFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ 72
           +F R+    +PA A     + ATELP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ
Sbjct: 34  KFSRTPNVNAPAPAPPLSAV-ATELPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ 93

Query: 73  AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG 132
           AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEG
Sbjct: 94  AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEG 153

Query: 133 SLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVK 192
            L GVTVCDAGCGTGSLSIPLAKEGA+VFASDISAAMVAEA+NKAKEEV GGVVPKFEVK
Sbjct: 154 PLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVK 213

Query: 193 DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE 252
           DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE
Sbjct: 214 DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE 273

Query: 253 LFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           LFPGPSKATRAYLHSEADVETALRR GWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 274 LFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVPA 323

BLAST of CsGy1G000060 vs. ExPASy TrEMBL
Match: A0A0A0LNQ2 (Mg-por_mtran_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G000570 PE=4 SV=1)

HSP 1 Score: 526 bits (1356), Expect = 2.96e-187
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0

Query: 34  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 93
           ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF
Sbjct: 48  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 107

Query: 94  QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 153
           QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL
Sbjct: 108 QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 167

Query: 154 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 213
           AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY
Sbjct: 168 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 227

Query: 214 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 273
           PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET
Sbjct: 228 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 287

Query: 274 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           ALRRAGWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 288 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 317

BLAST of CsGy1G000060 vs. ExPASy TrEMBL
Match: A0A5D3BF92 (Magnesium protoporphyrin IX methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00080 PE=4 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 1.29e-183
Identity = 265/270 (98.15%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 34  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 93
           ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF
Sbjct: 47  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 106

Query: 94  QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 153
           QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG LRGVTVCDAGCGTGSLSIPL
Sbjct: 107 QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPL 166

Query: 154 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 213
           AKEGA VFASDISAAMVAEAENKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHY
Sbjct: 167 AKEGAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHY 226

Query: 214 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 273
           PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET
Sbjct: 227 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 286

Query: 274 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           ALRR GWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 287 ALRRVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of CsGy1G000060 vs. ExPASy TrEMBL
Match: E5GBL6 (Magnesium-protoporphyrin O-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 1.29e-183
Identity = 265/270 (98.15%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 34  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 93
           ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF
Sbjct: 47  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 106

Query: 94  QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 153
           QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG LRGVTVCDAGCGTGSLSIPL
Sbjct: 107 QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPL 166

Query: 154 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 213
           AKEGA VFASDISAAMVAEAENKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHY
Sbjct: 167 AKEGAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHY 226

Query: 214 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 273
           PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET
Sbjct: 227 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 286

Query: 274 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           ALRR GWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 287 ALRRVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of CsGy1G000060 vs. ExPASy TrEMBL
Match: A0A1S3CMU6 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502274 PE=4 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 1.29e-183
Identity = 265/270 (98.15%), Postives = 266/270 (98.52%), Query Frame = 0

Query: 34  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 93
           ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF
Sbjct: 47  ATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGF 106

Query: 94  QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPL 153
           QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG LRGVTVCDAGCGTGSLSIPL
Sbjct: 107 QRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPL 166

Query: 154 AKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 213
           AKEGA VFASDISAAMVAEAENKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHY
Sbjct: 167 AKEGAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHY 226

Query: 214 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 273
           PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET
Sbjct: 227 PQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVET 286

Query: 274 ALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           ALRR GWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 287 ALRRVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of CsGy1G000060 vs. ExPASy TrEMBL
Match: A0A6J1JG16 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484791 PE=4 SV=1)

HSP 1 Score: 516 bits (1328), Expect = 6.78e-183
Identity = 267/291 (91.75%), Postives = 274/291 (94.16%), Query Frame = 0

Query: 13  QFRRSLTYFSPATAARKEQLFATELPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ 72
           +F R+    +PA A     + ATELP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ
Sbjct: 34  KFSRTPNVNAPAPAPPLSAV-ATELPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQ 93

Query: 73  AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEG 132
           AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEG
Sbjct: 94  AEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEG 153

Query: 133 SLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAKEEVVGGVVPKFEVK 192
            L GVTVCDAGCGTGSLSIPLAKEGA+VFASDISAAMVAEA+NKAKEEV GGVVPKFEVK
Sbjct: 154 PLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVK 213

Query: 193 DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE 252
           DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE
Sbjct: 214 DLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGE 273

Query: 253 LFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLVEAVPA 303
           LFPGPSKATRAYLHSEADVETALRR GWKIRKRGLISTQFYFATLVEAVPA
Sbjct: 274 LFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLISTQFYFATLVEAVPA 323

BLAST of CsGy1G000060 vs. TAIR 10
Match: AT4G25080.3 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 430.6 bits (1106), Expect = 1.0e-120
Identity = 224/305 (73.44%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 7   LSHSSSQFRRSLTYFSPAT---------AARKEQLFATELPIVDGATIAAIGGGSVAALA 66
           L  SSS   + L  F  AT         AA       T+L  VD  TIA +GGGSVAALA
Sbjct: 7   LLSSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALA 66

Query: 67  AVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGH 126
           A++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH
Sbjct: 67  AMVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGH 126

Query: 127 SKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKA 186
           +KTVEN M ML ++ SL GVTVCDAGCGTG LSIPLAKEGA+V ASDISAAMVAEAE KA
Sbjct: 127 AKTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKA 186

Query: 187 KEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISF 246
           K ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SF
Sbjct: 187 KAQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSF 246

Query: 247 APKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATL 303
           APKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL + GWKI KRGL +TQFYF+ L
Sbjct: 247 APKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRL 306

BLAST of CsGy1G000060 vs. TAIR 10
Match: AT4G25080.1 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 430.6 bits (1106), Expect = 1.0e-120
Identity = 224/305 (73.44%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 7   LSHSSSQFRRSLTYFSPAT---------AARKEQLFATELPIVDGATIAAIGGGSVAALA 66
           L  SSS   + L  F  AT         AA       T+L  VD  TIA +GGGSVAALA
Sbjct: 7   LLSSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALA 66

Query: 67  AVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGH 126
           A++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH
Sbjct: 67  AMVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGH 126

Query: 127 SKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKA 186
           +KTVEN M ML ++ SL GVTVCDAGCGTG LSIPLAKEGA+V ASDISAAMVAEAE KA
Sbjct: 127 AKTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKA 186

Query: 187 KEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISF 246
           K ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SF
Sbjct: 187 KAQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSF 246

Query: 247 APKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATL 303
           APKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL + GWKI KRGL +TQFYF+ L
Sbjct: 247 APKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRL 306

BLAST of CsGy1G000060 vs. TAIR 10
Match: AT4G25080.2 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 430.6 bits (1106), Expect = 1.0e-120
Identity = 224/305 (73.44%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 7   LSHSSSQFRRSLTYFSPAT---------AARKEQLFATELPIVDGATIAAIGGGSVAALA 66
           L  SSS   + L  F  AT         AA       T+L  VD  TIA +GGGSVAALA
Sbjct: 7   LLSSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALA 66

Query: 67  AVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGH 126
           A++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH
Sbjct: 67  AMVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGH 126

Query: 127 SKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKA 186
           +KTVEN M ML ++ SL GVTVCDAGCGTG LSIPLAKEGA+V ASDISAAMVAEAE KA
Sbjct: 127 AKTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKA 186

Query: 187 KEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISF 246
           K ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SF
Sbjct: 187 KAQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSF 246

Query: 247 APKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATL 303
           APKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL + GWKI KRGL +TQFYF+ L
Sbjct: 247 APKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRL 306

BLAST of CsGy1G000060 vs. TAIR 10
Match: AT4G25080.5 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 430.6 bits (1106), Expect = 1.0e-120
Identity = 224/305 (73.44%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 7   LSHSSSQFRRSLTYFSPAT---------AARKEQLFATELPIVDGATIAAIGGGSVAALA 66
           L  SSS   + L  F  AT         AA       T+L  VD  TIA +GGGSVAALA
Sbjct: 7   LLSSSSSVSQFLPRFPNATRFNVTPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALA 66

Query: 67  AVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGH 126
           A++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH
Sbjct: 67  AMVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGH 126

Query: 127 SKTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKA 186
           +KTVEN M ML ++ SL GVTVCDAGCGTG LSIPLAKEGA+V ASDISAAMVAEAE KA
Sbjct: 127 AKTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKA 186

Query: 187 KEEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISF 246
           K ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SF
Sbjct: 187 KAQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSF 246

Query: 247 APKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATL 303
           APKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL + GWKI KRGL +TQFYF+ L
Sbjct: 247 APKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRL 306

BLAST of CsGy1G000060 vs. TAIR 10
Match: AT4G25080.4 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 404.4 bits (1038), Expect = 7.8e-113
Identity = 197/244 (80.74%), Postives = 223/244 (91.39%), Query Frame = 0

Query: 59  VLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHS 118
           ++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+
Sbjct: 1   MVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHA 60

Query: 119 KTVENVMAMLKDEGSLRGVTVCDAGCGTGSLSIPLAKEGAMVFASDISAAMVAEAENKAK 178
           KTVEN M ML ++ SL GVTVCDAGCGTG LSIPLAKEGA+V ASDISAAMVAEAE KAK
Sbjct: 61  KTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAK 120

Query: 179 EEVVGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFA 238
            ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFA
Sbjct: 121 AQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFA 180

Query: 239 PKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRAGWKIRKRGLISTQFYFATLV 298
           PKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL + GWKI KRGL +TQFYF+ L+
Sbjct: 181 PKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLI 240

Query: 299 EAVP 303
           EAVP
Sbjct: 241 EAVP 244

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SW181.4e-11973.44Magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Arabidopsis thal... [more]
Q554674.0e-6957.21Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 68... [more]
P262369.3e-1828.18Magnesium-protoporphyrin O-methyltransferase OS=Rhodobacter capsulatus OX=1061 G... [more]
A7MPA95.3e-0532.00Ubiquinone biosynthesis O-methyltransferase OS=Cronobacter sakazakii (strain ATC... [more]
Q2NSL75.3e-0532.23Ubiquinone biosynthesis O-methyltransferase OS=Sodalis glossinidius (strain mors... [more]
Match NameE-valueIdentityDescription
KGN63436.24.34e-211100.00hypothetical protein Csa_013123 [Cucumis sativus][more]
XP_004138019.16.12e-187100.00magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucumis sativus][more]
XP_008464364.12.67e-18398.15PREDICTED: magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucumis... [more]
XP_022987155.11.40e-18291.75magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita maxima][more]
XP_023516229.11.99e-18291.41magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita pepo sub... [more]
Match NameE-valueIdentityDescription
A0A0A0LNQ22.96e-187100.00Mg-por_mtran_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G000... [more]
A0A5D3BF921.29e-18398.15Magnesium protoporphyrin IX methyltransferase OS=Cucumis melo var. makuwa OX=119... [more]
E5GBL61.29e-18398.15Magnesium-protoporphyrin O-methyltransferase OS=Cucumis melo subsp. melo OX=4126... [more]
A0A1S3CMU61.29e-18398.15magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucumis melo OX=... [more]
A0A6J1JG166.78e-18391.75magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT4G25080.31.0e-12073.44magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.11.0e-12073.44magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.21.0e-12073.44magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.51.0e-12073.44magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.47.8e-11380.74magnesium-protoporphyrin IX methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010940Magnesium-protoporphyrin IX methyltransferase, C-terminalPFAMPF07109Mg-por_mtran_Ccoord: 206..301
e-value: 2.4E-35
score: 120.8
IPR010251Magnesium-protoporphyrin IX methyltransferaseTIGRFAMTIGR02021TIGR02021coord: 80..299
e-value: 4.6E-87
score: 289.0
IPR010251Magnesium-protoporphyrin IX methyltransferasePROSITEPS51556SAM_MT_MG_PIXcoord: 1..302
score: 97.37133
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 77..303
e-value: 8.7E-67
score: 226.9
NoneNo IPR availablePIRSRPIRSR000385-2PIRSR000385-2coord: 122..208
e-value: 9.7E-9
score: 32.8
NoneNo IPR availablePANTHERPTHR43591:SF32MAGNESIUM PROTOPORPHYRIN IX METHYLTRANSFERASE, CHLOROPLASTIC-RELATEDcoord: 45..299
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 45..299
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 138..237
e-value: 4.48651E-8
score: 48.5803
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 76..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy1G000060.2CsGy1G000060.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0032259 methylation
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046406 magnesium protoporphyrin IX methyltransferase activity