Cp4.1LG20g08850 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g08850
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionActin depolymerizing factor-like protein
LocationCp4.1LG20: 7053640 .. 7057177 (+)
RNA-Seq ExpressionCp4.1LG20g08850
SyntenyCp4.1LG20g08850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTGGCGTTTGATTGGAGATACCCGCTCCAACTCCGCTGTCGCGCATGACCGCCATCATGATATTGTGGAAAAAGTAAAGCCACCGCTTACCAAGAGAGCAAGAATAGAAGGAGAGAGAGAGAGAGAGAAGAATATCTCAAAAACATTTCGCGATTTCTTTTCTACCCATCTCGCAGCTAAAGATTGTTCTTCCTCTTCTTCCGTTTCTCTTCCTAGATCCTCTGATTCAATTTCCCTACTCAAGCTTCAGCCATGGTAAGCCGCTTTTTACTGATTCCTGTTCTTTTGCTGATTTCTACTTCATTTGTTTCTTGATTTTGTACTCGATTTCTCTTCTTTTTGCTTGATTTTGATCTCTGATGCTTGTGGATGTGCTTTTAGGACCTGCTTGCTGTGGATCTATATTCTGTTCTTGTTTTGATCTAGATTGTTTGAGTTTTTTTGTCCGATTTGCCTCGGGATCGGATTTGGTTGATTTTAAATTTGTTTAGGATTGATTTCAGTATCATCGGAAGTACTTGGATGTGATCGTGACTGATCTCTGATTACCGTGGTTTAGTAGTCTCTCAAAATTGATCGTTTGTGTTTTAATCGGGCTGGGAGCTGGGTTTCGGTGTCTTGTTGTGCTTGGTTCTGTGGATTTAGATGTTTTGGTGATTGAGATGATGTTTTGGTAGTTGGTGGAAAGATGGTGATACTTCGCGTATCATCAGGATGGTAATCTTGTGAAGTGAATGGAGTGCGTGTATTACTGTTTGATAATCAGGTACATGATAATTTCTCGGCTGGGGCGGAGCGGGGTGCCCTCAAATGAGTCCCTAACAGACTGAGAAATAGAGGGAAGAATTGGGAATGAAATGGATTTTTTATATTTAATCATGATGATGCTCACTTTCAATTTTCAGTATTGCTTCAAATTTGAAAGTTTATACTGCTCTCTTGAGAGTGAAAATGTAACGCTATAGCCAATAGGCTGTTGAACACAACTCTTTGTGGGAAACACTCGCACTCTTTATTAATATCAATCGAGAAGAGAATACAATACACTCGGTAGAATACTACACTTTGCTTTAATTGAGACTGCTTGGGCTTGGATTTCCTATGGATATTTATAGGCAACTACTCGGTTATGCCAAAAGCAATGAATGTTATCTCTATGATTGTGAGAATAGACTCTAAAGATCCATGTGGAGATCATACTGGTAACCAAGCAGATTCTATAAATATCCAACTCTAAGGGGAATTTTACTAAAAGTGATGAAGGGAAGTTTGAATTACTCATTCAGACATTTGATTTTGCATACTTTTCAGAGCTTTGGCTCATTGCCCTTAATTTATATTTTCAATTACTTACATTTTTTTTTTCCTGCAGGCTAACGCGGCATCGGGTATGGCTGTGCACGATGACTGTAAGCTTAAGTTCTTGGAGTTGAAGACCAAGAGAACTTACAGATTCATTGTATATAAGATTGAAGAGAAGCAGAAGCAAGTCATTGTGGAAAAGGTTGGCGAGCCAGGTCAAAGCTATGAAGATTTCACAGCCTGCCTTCCTGCTGATGAATGCCGATATGCGGTTTATGATTTTGACTTTCTGACTAAGGAGAATGTTCAGAAGAGCAGAATTTTCTTCATTGCTTGGTAATGTCATGATAAATGAAAAAACAAGCAAATTCTGTGTTATTCTCTGTTCCTCACCGGACCCGACCACGTATTAAACTTCCATCACTAATCATCATTATTCTCTTTTCTTGTTGCAAAGGTCTCCCGACGTGTCGAAGGTAAGAAGCAAGATGATCTATGCAAGCTCAAAGGACAAGTTTAGAAGAGAATTGGATGGCATTCAAATTGAGCTGCAAGCTACTGACCCATCTGAAATGGATCTCGATGTTTTTAAGAGCCGTGCGAAATAAGAAGCGTCTTCGTTATGAACTTCTTGTGGTGTCTGTTTTACATTGTGAAATGATGTTATTAATTGCTGGGTTTATGAATGTGCTTTGATTACATATTGGGTTGTTTTCTAAGTTTTGTCTTATAGAATTATGTCCATCTGGAACAATCTTCGGTCATTTTTCCCCCCCACCTTATATTCTATGATTTATCGTGTGGAATTAATGCAATATCTCCTTATGTAACGCTACATGATTTTGGTCTGGATTTACATCTTTTATCATGCACAGCACATTTAAAGATAATGTTGACCTTTTTTTTTCTAGATCCAAGGATGCTTCTGAGCTGAATGGTTGCAATGTCAACGCTTGCTAGACAGCACAGCAGCATAGGCTTTCTAACCGGGTTGACTCGATTCTCATTTGGTAGCTCCTCTCTTCTTTGATCCTTATTATCAAAATTATTGAAGAATTTAGATTTCATTAGCATTTTGCAACAGTGAAGTAAAAGAATCCATGATATTCTAGGACTACAATAAAGATTGATCCATCTTCTTTAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAATAGGGCTTAACCATTTCTACAATATGCCTCTATTTTCCCAGCTTCTAGTGTTGGATTTGAAGGTTTATTCAGTTTGGCCAAGCTTCAAACCTTTTTTAAAGAATCAAATAATGGAAGATAGGCTAAATTATAGGTATATATGTTTGAGAAGTGTTCACATGGAAATCCAGGTGTTCAAATAGGCCTTTGCGAAGTGTTCACATGGAAGTCCAGGTGTTCAAATAGGCCTCCAACACATCTCTAGCCAAGTTTAGGCTTCATAAACCTTGAATCTTTCACATAGGAGACTATATAATAGCCTTCTGTTACCTACGGAGCTTTACAATGATATGATATTGTCCACTTTGAGCATAAGCTCTCGTGACTTTGCTTTTGGATTTTCCGAAAGATCTCGTACCATAAAGATAGCGTTCCTTTTTTATAAATTCGTGATCTTCCTCTTAATTAACTAATATGAGACTACTCATCTCAATAATCCGCACCACTTTCACGTTTAGGTATCTTGTCTTCTGGGTGAACTCCTCCAAAATTTTATCTATAAATTTGGGGAACTTGCAAAGTATCTGTGGATAAGTATTATGATCAGCTATATGCCCAAGGTTGAGCAATTTGTGTGGGTTTGTCTTCTATACACGAAAGGCACTGCCCACATATTCCCACTTCATCTGATGTTCAACATCTTATTCCTTAACAACCAATTGCACTTCTGAAGATTCTCTGCTAGTGACTTGATTTTCGAGTTCAATTTGTTGTGTAGCTTCATTATAAGAAATGTTGCGTAAGAAAAAGGAGGTATTTGTTGTGAGAGACAACATATAGGTTTTGGAAACTAAGAGACTTACATGAAAGATTGATTAGCGGAAGGTGAGTTTTCATCTTCAAAAGTAAGAAACTTATCGAACAATAGTGACATTTAAGTTCGATTGTTTTGATCTTGTTTTGTTTCGTTTCTTCCCTCTCATTTGTTCACGTCTTAACCATGATTTTTGTCAATTAATCGTGAGAGGGGAGGCTC

mRNA sequence

TGGTGGCGTTTGATTGGAGATACCCGCTCCAACTCCGCTGTCGCGCATGACCGCCATCATGATATTGTGGAAAAAGTAAAGCCACCGCTTACCAAGAGAGCAAGAATAGAAGGAGAGAGAGAGAGAGAGAAGAATATCTCAAAAACATTTCGCGATTTCTTTTCTACCCATCTCGCAGCTAAAGATTGTTCTTCCTCTTCTTCCGTTTCTCTTCCTAGATCCTCTGATTCAATTTCCCTACTCAAGCTTCAGCCATGGCTAACGCGGCATCGGGTATGGCTGTGCACGATGACTGTAAGCTTAAGTTCTTGGAGTTGAAGACCAAGAGAACTTACAGATTCATTGTATATAAGATTGAAGAGAAGCAGAAGCAAGTCATTGTGGAAAAGGTTGGCGAGCCAGGTCAAAGCTATGAAGATTTCACAGCCTGCCTTCCTGCTGATGAATGCCGATATGCGGTTTATGATTTTGACTTTCTGACTAAGGAGAATGTTCAGAAGAGCAGAATTTTCTTCATTGCTTGGTCTCCCGACGTGTCGAAGGTAAGAAGCAAGATGATCTATGCAAGCTCAAAGGACAAGTTTAGAAGAGAATTGGATGGCATTCAAATTGAGCTGCAAGCTACTGACCCATCTGAAATGGATCTCGATGTTTTTAAGAGCCGTGCGAAATAAGAAGCGTCTTCGTTATGAACTTCTTGTGGTGTCTGTTTTACATTGTGAAATGATGTTATTAATTGCTGGGTTTATGAATGTGCTTTGATTACATATTGGGTTGTTTTCTAAGTTTTGTCTTATAGAATTATGTCCATCTGGAACAATCTTCGGTCATTTTTCCCCCCCACCTTATATTCTATGATTTATCGTGTGGAATTAATGCAATATCTCCTTATGTAACGCTACATGATTTTGGTCTGGATTTACATCTTTTATCATGCACAGCACATTTAAAGATAATGTTGACCTTTTTTTTTCTAGATCCAAGGATGCTTCTGAGCTGAATGGTTGCAATGTCAACGCTTGCTAGACAGCACAGCAGCATAGGCTTTCTAACCGGGTTGACTCGATTCTCATTTGGTGTTCAAATAGGCCTTTGCGAAGTGTTCACATGGAAGTCCAGGTGTTCAAATAGGCCTCCAACACATCTCTAGCCAAGTTTAGGCTTCATAAACCTTGAATCTTTCACATAGGAGACTATATAATAGCCTTCTGTTACCTACGGAGCTTTACAATGATATGATATTGTCCACTTTGAGCATAAGCTCTCGTGACTTTGCTTTTGGATTTTCCGAAAGATCTCGTACCATAAAGATAGCGTTCCTTTTTTATAAATTCGTGATCTTCCTCTTAATTAACTAATATGAGACTACTCATCTCAATAATCCGCACCACTTTCACGTTTAGGTATCTTGTCTTCTGGGTGAACTCCTCCAAAATTTTATCTATAAATTTGGGGAACTTGCAAAGTATCTGTGGATAAGTATTATGATCAGCTATATGCCCAAGGTTGAGCAATTTGTGTGGGTTTGTCTTCTATACACGAAAGGCACTGCCCACATATTCCCACTTCATCTGATGTTCAACATCTTATTCCTTAACAACCAATTGCACTTCTGAAGATTCTCTGCTAGTGACTTGATTTTCGAGTTCAATTTGTTGTGTAGCTTCATTATAAGAAATGTTGCGTAAGAAAAAGGAGGTATTTGTTGTGAGAGACAACATATAGGTTTTGGAAACTAAGAGACTTACATGAAAGATTGATTAGCGGAAGGTGAGTTTTCATCTTCAAAAGTAAGAAACTTATCGAACAATAGTGACATTTAAGTTCGATTGTTTTGATCTTGTTTTGTTTCGTTTCTTCCCTCTCATTTGTTCACGTCTTAACCATGATTTTTGTCAATTAATCGTGAGAGGGGAGGCTC

Coding sequence (CDS)

ATGGCTAACGCGGCATCGGGTATGGCTGTGCACGATGACTGTAAGCTTAAGTTCTTGGAGTTGAAGACCAAGAGAACTTACAGATTCATTGTATATAAGATTGAAGAGAAGCAGAAGCAAGTCATTGTGGAAAAGGTTGGCGAGCCAGGTCAAAGCTATGAAGATTTCACAGCCTGCCTTCCTGCTGATGAATGCCGATATGCGGTTTATGATTTTGACTTTCTGACTAAGGAGAATGTTCAGAAGAGCAGAATTTTCTTCATTGCTTGGTCTCCCGACGTGTCGAAGGTAAGAAGCAAGATGATCTATGCAAGCTCAAAGGACAAGTTTAGAAGAGAATTGGATGGCATTCAAATTGAGCTGCAAGCTACTGACCCATCTGAAATGGATCTCGATGTTTTTAAGAGCCGTGCGAAATAA

Protein sequence

MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACLPADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIELQATDPSEMDLDVFKSRAK
Homology
BLAST of Cp4.1LG20g08850 vs. ExPASy Swiss-Prot
Match: Q9FVI1 (Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 4.6e-65
Identity = 119/138 (86.23%), Postives = 132/138 (95.65%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELK KRTYRFI+YKIEEKQK+V+VEK+GEP +SYEDFTA L
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDF+TKEN QKSRIFFIAWSPD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSRA 139
           LQATDP+EM LDVF+SRA
Sbjct: 121 LQATDPTEMGLDVFRSRA 138

BLAST of Cp4.1LG20g08850 vs. ExPASy Swiss-Prot
Match: Q39250 (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.3e-64
Identity = 116/138 (84.06%), Postives = 132/138 (95.65%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQV+VEKVG+P Q+YE+F ACL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PD++KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSRA 139
           LQATDP+EMDLDVF+SRA
Sbjct: 121 LQATDPTEMDLDVFRSRA 138

BLAST of Cp4.1LG20g08850 vs. ExPASy Swiss-Prot
Match: Q9ZSK3 (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 9.6e-63
Identity = 117/137 (85.40%), Postives = 128/137 (93.43%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQVIVEKVGEP  +YEDF A L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PDV+KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSR 138
           LQATDP+EMDLDV KSR
Sbjct: 121 LQATDPTEMDLDVLKSR 137

BLAST of Cp4.1LG20g08850 vs. ExPASy Swiss-Prot
Match: Q9ZSK4 (Actin-depolymerizing factor 3 OS=Arabidopsis thaliana OX=3702 GN=ADF3 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 2.1e-62
Identity = 112/138 (81.16%), Postives = 130/138 (94.20%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKF+ELKTKRT+RFI+YKIEE QKQVIVEK+GEPGQ++ED  A L
Sbjct: 1   MANAASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA++DFDF++ E V +SRIFF+AWSPD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIFDFDFVSSEGVPRSRIFFVAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSRA 139
           LQATDP+EMDLDVFKSRA
Sbjct: 121 LQATDPTEMDLDVFKSRA 138

BLAST of Cp4.1LG20g08850 vs. ExPASy Swiss-Prot
Match: Q9FVI2 (Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.2e-60
Identity = 112/138 (81.16%), Postives = 126/138 (91.30%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQV+VEK+GEP +SYEDF A L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           P +ECRYAVYDFDF+T EN QKSRIFFIAW PD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSRA 139
           LQA DP+EM LDV +SRA
Sbjct: 121 LQACDPTEMGLDVIQSRA 138

BLAST of Cp4.1LG20g08850 vs. NCBI nr
Match: XP_022964654.1 (actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_022970372.1 actin-depolymerizing factor 2-like [Cucurbita maxima] >XP_023519424.1 actin-depolymerizing factor 2-like [Cucurbita pepo subsp. pepo] >KAG6583469.1 Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019224.1 Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 277 bits (708), Expect = 8.00e-94
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDPSEMDLDVFKSRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

BLAST of Cp4.1LG20g08850 vs. NCBI nr
Match: XP_022156171.1 (actin-depolymerizing factor 2 [Momordica charantia])

HSP 1 Score: 268 bits (686), Expect = 1.81e-90
Identity = 133/139 (95.68%), Postives = 137/139 (98.56%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELKTKRTYR+IVYKIEEKQKQVIVEK+GEPGQSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKLGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPD+SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

BLAST of Cp4.1LG20g08850 vs. NCBI nr
Match: XP_022997831.1 (actin-depolymerizing factor 2-like [Cucurbita maxima])

HSP 1 Score: 266 bits (680), Expect = 1.49e-89
Identity = 133/139 (95.68%), Postives = 136/139 (97.84%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQVIVEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

BLAST of Cp4.1LG20g08850 vs. NCBI nr
Match: XP_022948707.1 (actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023521573.1 actin-depolymerizing factor 2-like [Cucurbita pepo subsp. pepo] >XP_023523187.1 actin-depolymerizing factor 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023523188.1 actin-depolymerizing factor 2-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6607202.1 Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036888.1 Actin-depolymerizing factor 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 266 bits (679), Expect = 2.12e-89
Identity = 132/139 (94.96%), Postives = 136/139 (97.84%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQVIVEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPD+SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

BLAST of Cp4.1LG20g08850 vs. NCBI nr
Match: XP_008457551.1 (PREDICTED: actin-depolymerizing factor 2 [Cucumis melo] >ADN34239.1 actin depolymerizing factor-like protein [Cucumis melo subsp. melo] >KAA0033926.1 actin-depolymerizing factor 2 [Cucumis melo var. makuwa] >TYK02902.1 actin-depolymerizing factor 2 [Cucumis melo var. makuwa])

HSP 1 Score: 265 bits (678), Expect = 3.01e-89
Identity = 133/139 (95.68%), Postives = 135/139 (97.12%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQV VEKVGEPGQSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVTVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDF+FLT+ NV KSRIFFIAWSPD SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFEFLTEGNVPKSRIFFIAWSPDTSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDPSEMDLDVFKSRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

BLAST of Cp4.1LG20g08850 vs. ExPASy TrEMBL
Match: A0A6J1HYW9 (actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111469374 PE=3 SV=1)

HSP 1 Score: 277 bits (708), Expect = 3.87e-94
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDPSEMDLDVFKSRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

BLAST of Cp4.1LG20g08850 vs. ExPASy TrEMBL
Match: A0A6J1HNW0 (actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464666 PE=3 SV=1)

HSP 1 Score: 277 bits (708), Expect = 3.87e-94
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDPSEMDLDVFKSRAK
Sbjct: 121 LQATDPSEMDLDVFKSRAK 139

BLAST of Cp4.1LG20g08850 vs. ExPASy TrEMBL
Match: A0A6J1DU41 (actin-depolymerizing factor 2 OS=Momordica charantia OX=3673 GN=LOC111023124 PE=3 SV=1)

HSP 1 Score: 268 bits (686), Expect = 8.78e-91
Identity = 133/139 (95.68%), Postives = 137/139 (98.56%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKFLELKTKRTYR+IVYKIEEKQKQVIVEK+GEPGQSYEDFTACL
Sbjct: 1   MANAASGMAVHDDCKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKLGEPGQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPD+SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

BLAST of Cp4.1LG20g08850 vs. ExPASy TrEMBL
Match: A0A6J1K8K8 (actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492671 PE=3 SV=1)

HSP 1 Score: 266 bits (680), Expect = 7.22e-90
Identity = 133/139 (95.68%), Postives = 136/139 (97.84%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQVIVEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

BLAST of Cp4.1LG20g08850 vs. ExPASy TrEMBL
Match: A0A6J1GA03 (actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452299 PE=3 SV=1)

HSP 1 Score: 266 bits (679), Expect = 1.03e-89
Identity = 132/139 (94.96%), Postives = 136/139 (97.84%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHD+CKLKFLELKTKRTYR+IVYKIEEKQKQVIVEKVGEP QSYEDFTACL
Sbjct: 1   MANAASGMAVHDECKLKFLELKTKRTYRYIVYKIEEKQKQVIVEKVGEPSQSYEDFTACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYAVYDFDFLTKENVQKSRIFFIAWSPD+SKVRSKMIYASSKDKFRRELDGIQIE
Sbjct: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDISKVRSKMIYASSKDKFRRELDGIQIE 120

Query: 121 LQATDPSEMDLDVFKSRAK 139
           LQATDP+EM LDVF SRAK
Sbjct: 121 LQATDPTEMGLDVFNSRAK 139

BLAST of Cp4.1LG20g08850 vs. TAIR 10
Match: AT3G46010.1 (actin depolymerizing factor 1 )

HSP 1 Score: 246.9 bits (629), Expect = 9.5e-66
Identity = 116/138 (84.06%), Postives = 132/138 (95.65%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQV+VEKVG+P Q+YE+F ACL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PD++KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSRA 139
           LQATDP+EMDLDVF+SRA
Sbjct: 121 LQATDPTEMDLDVFRSRA 138

BLAST of Cp4.1LG20g08850 vs. TAIR 10
Match: AT3G46010.2 (actin depolymerizing factor 1 )

HSP 1 Score: 245.0 bits (624), Expect = 3.6e-65
Identity = 115/137 (83.94%), Postives = 131/137 (95.62%), Query Frame = 0

Query: 2   ANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACLP 61
           ANAASGMAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQV+VEKVG+P Q+YE+F ACLP
Sbjct: 13  ANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLP 72

Query: 62  ADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIEL 121
           ADECRYA+YDFDF+T EN QKS+IFFIAW PD++KVRSKMIYASSKD+F+RELDGIQ+EL
Sbjct: 73  ADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVEL 132

Query: 122 QATDPSEMDLDVFKSRA 139
           QATDP+EMDLDVF+SRA
Sbjct: 133 QATDPTEMDLDVFRSRA 149

BLAST of Cp4.1LG20g08850 vs. TAIR 10
Match: AT5G59890.1 (actin depolymerizing factor 4 )

HSP 1 Score: 240.7 bits (613), Expect = 6.8e-64
Identity = 117/137 (85.40%), Postives = 128/137 (93.43%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQVIVEKVGEP  +YEDF A L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA+YDFDF+T EN QKS+IFFIAW PDV+KVRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSR 138
           LQATDP+EMDLDV KSR
Sbjct: 121 LQATDPTEMDLDVLKSR 137

BLAST of Cp4.1LG20g08850 vs. TAIR 10
Match: AT5G59880.1 (actin depolymerizing factor 3 )

HSP 1 Score: 239.6 bits (610), Expect = 1.5e-63
Identity = 112/138 (81.16%), Postives = 130/138 (94.20%), Query Frame = 0

Query: 1   MANAASGMAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACL 60
           MANAASGMAVHDDCKLKF+ELKTKRT+RFI+YKIEE QKQVIVEK+GEPGQ++ED  A L
Sbjct: 1   MANAASGMAVHDDCKLKFMELKTKRTHRFIIYKIEELQKQVIVEKIGEPGQTHEDLAASL 60

Query: 61  PADECRYAVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIE 120
           PADECRYA++DFDF++ E V +SRIFF+AWSPD ++VRSKMIYASSKD+F+RELDGIQ+E
Sbjct: 61  PADECRYAIFDFDFVSSEGVPRSRIFFVAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 121 LQATDPSEMDLDVFKSRA 139
           LQATDP+EMDLDVFKSRA
Sbjct: 121 LQATDPTEMDLDVFKSRA 138

BLAST of Cp4.1LG20g08850 vs. TAIR 10
Match: AT5G59890.2 (actin depolymerizing factor 4 )

HSP 1 Score: 228.0 bits (580), Expect = 4.6e-60
Identity = 110/130 (84.62%), Postives = 121/130 (93.08%), Query Frame = 0

Query: 8   MAVHDDCKLKFLELKTKRTYRFIVYKIEEKQKQVIVEKVGEPGQSYEDFTACLPADECRY 67
           MAVHDDCKL+FLELK KRT+RFIVYKIEEKQKQVIVEKVGEP  +YEDF A LPADECRY
Sbjct: 1   MAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECRY 60

Query: 68  AVYDFDFLTKENVQKSRIFFIAWSPDVSKVRSKMIYASSKDKFRRELDGIQIELQATDPS 127
           A+YDFDF+T EN QKS+IFFIAW PDV+KVRSKMIYASSKD+F+RELDGIQ+ELQATDP+
Sbjct: 61  AIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 120

Query: 128 EMDLDVFKSR 138
           EMDLDV KSR
Sbjct: 121 EMDLDVLKSR 130

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FVI14.6e-6586.23Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1[more]
Q392501.3e-6484.06Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1[more]
Q9ZSK39.6e-6385.40Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2[more]
Q9ZSK42.1e-6281.16Actin-depolymerizing factor 3 OS=Arabidopsis thaliana OX=3702 GN=ADF3 PE=1 SV=1[more]
Q9FVI21.2e-6081.16Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022964654.18.00e-94100.00actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_022970372.1 actin-de... [more]
XP_022156171.11.81e-9095.68actin-depolymerizing factor 2 [Momordica charantia][more]
XP_022997831.11.49e-8995.68actin-depolymerizing factor 2-like [Cucurbita maxima][more]
XP_022948707.12.12e-8994.96actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023521573.1 actin-de... [more]
XP_008457551.13.01e-8995.68PREDICTED: actin-depolymerizing factor 2 [Cucumis melo] >ADN34239.1 actin depoly... [more]
Match NameE-valueIdentityDescription
A0A6J1HYW93.87e-94100.00actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111469374 P... [more]
A0A6J1HNW03.87e-94100.00actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464666... [more]
A0A6J1DU418.78e-9195.68actin-depolymerizing factor 2 OS=Momordica charantia OX=3673 GN=LOC111023124 PE=... [more]
A0A6J1K8K87.22e-9095.68actin-depolymerizing factor 2-like OS=Cucurbita maxima OX=3661 GN=LOC111492671 P... [more]
A0A6J1GA031.03e-8994.96actin-depolymerizing factor 2-like OS=Cucurbita moschata OX=3662 GN=LOC111452299... [more]
Match NameE-valueIdentityDescription
AT3G46010.19.5e-6684.06actin depolymerizing factor 1 [more]
AT3G46010.23.6e-6583.94actin depolymerizing factor 1 [more]
AT5G59890.16.8e-6485.40actin depolymerizing factor 4 [more]
AT5G59880.11.5e-6381.16actin depolymerizing factor 3 [more]
AT5G59890.24.6e-6084.62actin depolymerizing factor 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002108Actin-depolymerising factor homology domainSMARTSM00102adf_2coord: 12..139
e-value: 1.1E-58
score: 210.9
IPR002108Actin-depolymerising factor homology domainPFAMPF00241Cofilin_ADFcoord: 14..136
e-value: 4.6E-37
score: 126.6
IPR002108Actin-depolymerising factor homology domainPROSITEPS51263ADF_Hcoord: 5..139
score: 37.663551
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 1..139
e-value: 1.6E-52
score: 178.7
IPR017904ADF/CofilinPANTHERPTHR11913COFILIN-RELATEDcoord: 1..138
IPR017904ADF/CofilinCDDcd11286ADF_cofilin_likecoord: 6..138
e-value: 2.18528E-64
score: 190.461
NoneNo IPR availablePANTHERPTHR11913:SF74ACTIN-DEPOLYMERIZING FACTOR 2-LIKEcoord: 1..138
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 3..138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g08850.1Cp4.1LG20g08850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030042 actin filament depolymerization
cellular_component GO:0015629 actin cytoskeleton
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051015 actin filament binding
molecular_function GO:0003779 actin binding