Cp4.1LG20g08120 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g08120
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Location	Cp4.1LG20: 7795144 .. 7796404 (-)
RNA-Seq Expression	Cp4.1LG20g08120
Synteny	Cp4.1LG20g08120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGCCAATGGCGAACTGGTAGCTGTCATTTTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATCTCTTGCAATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCCTTCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGACTCCCAGGATTGCTCCGACCAGAACAAGGCCATGTTGGCGAACGCCCTCGATGCTATTCTCCCGTCGGCGCTGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCGGCGGCGGAGGAGGGGAGGTGGTTTATTGTGAGGACAGCCCGAGAGCGGAGTCCGTGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCCATTTGCAAGAAGTTGAACATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGACGGCTCCGACGCTCGGCTCACGGCGGAGCAGCACGTGTGGGCCGCAACAAAAGCCGTTGAAATAAATGGTATGTAACACCTACATCGAGCTGTCATAAATATAAATATATATTTTTTTTTTTTATAAGAGACAATTTCATTCATGATTGATAAATATAAATTTAATATCTGTTAACATGTAAAAATACCGTAATCTCAGATGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAAGAGATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGCGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTTCATTATACGGCAGCTATGGCGGAGAAAGGGACGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGAAAATGGGGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAGTCCGACCGGTTAGGGTTCACTGTTAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCT

mRNA sequence

ACGCCAATGGCGAACTGGTAGCTGTCATTTTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATCTCTTGCAATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCCTTCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGACTCCCAGGATTGCTCCGACCAGAACAAGGCCATGTTGGCGAACGCCCTCGATGCTATTCTCCCGTCGGCGCTGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCGGCGGCGGAGGAGGGGAGGTGGTTTATTGTGAGGACAGCCCGAGAGCGGAGTCCGTGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCCATTTGCAAGAAGTTGAACATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGACGGCTCCGACGCTCGGCTCACGGCGGAGCAGCACGTGTGGGCCGCAACAAAAGCCATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGCGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTTCATTATACGGCAGCTATGGCGGAGAAAGGGACGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGAAAATGGGGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAGTCCGACCGGTTAGGGTTCACTGTTAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCT

Coding sequence (CDS)

ATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATCTCTTGCAATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCCTTCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGACTCCCAGGATTGCTCCGACCAGAACAAGGCCATGTTGGCGAACGCCCTCGATGCTATTCTCCCGTCGGCGCTGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCGGCGGCGGAGGAGGGGAGGTGGTTTATTGTGAGGACAGCCCGAGAGCGGAGTCCGTGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCCATTTGCAAGAAGTTGAACATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGACGGCTCCGACGCTCGGCTCACGGCGGAGCAGCACGTGTGGGCCGCAACAAAAGCCATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGCGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTTCATTATACGGCAGCTATGGCGGAGAAAGGGACGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGAAAATGGGGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAGTCCGACCGGTTAGGGTTCACTGTTAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCT

Protein sequence

MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP

Homology

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BLAST of Cp4.1LG20g08120 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.7e-52
Identity = 132/344 (38.37%), Postives = 171/344 (49.71%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP        P   +I C++ D    H  LSPL  VTH+FW+TWA   R   
Sbjct: 56  WKVYGVARRPRPAWNADHP-VEYIQCDISDASDTHTKLSPLTDVTHIFWVTWAN--RPTE 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGG-GGGEVVYCEDSPR 127
            +C + N  ML N L+A++P A  L H+ LQTG KHY     + G     E  + ED PR
Sbjct: 116 SECCELNGTMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAFGKIKPHEPPFTEDMPR 175

Query: 128 AESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKK 187
             +  NFYY LED+L E  S  +  + WS+ RP +I G S  +  N +G+LCVY AICK 
Sbjct: 176 L-NAPNFYYTLEDMLVE-ASEKKAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKH 235

Query: 188 LNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWG 247
            N P  F GT A W   Y   SDA L AE  +WAA                    K +W 
Sbjct: 236 ENTPLKFPGTKAAW-NCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWK 295

Query: 248 AVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDS 307
            +AE+ GV     DE            M +KG VW EIV E GL  TK+  +A W F D 
Sbjct: 296 VLAEQFGVEYAEFDES--EKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADV 355

Query: 308 LFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           +    E ++ S  KS   G+       +S++  ID M+  K++P
Sbjct: 356 ILG-GECLLDSMNKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.6e-50
Identity = 125/343 (36.44%), Postives = 172/343 (50.15%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP        P   +I C++ D       LSPL  VTH+F++TW  +   +S
Sbjct: 56  WKVYGVARRPRPTWNADHP-IDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNR-ESES 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA 127
           ++C + N +ML N L AI+P A  LRHV LQTG KHY    ++  G   +  + ED PR 
Sbjct: 116 ENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRL 175

Query: 128 ESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL 187
           + + NFYY  ED+L E +      V WSI RP +I G S  +  N +G+LCVY AICK  
Sbjct: 176 Q-IQNFYYTQEDILFEEIKKI-ETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHE 235

Query: 188 NMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWGA 247
             P +F G+   WE  ++  SDA L AEQ +WAA                    K +W  
Sbjct: 236 GSPLLFPGSKKAWEG-FMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKI 295

Query: 248 VAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSL 307
           +AE+ G+     +E            M  K  VW E+V+E  L   K+ E+  W F D +
Sbjct: 296 LAEQFGIEEYGFEE---GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVI 355

Query: 308 FRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
             + E M+ S  KS   GF       +S + WID  +  K++P
Sbjct: 356 LGV-EGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

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BLAST of Cp4.1LG20g08120 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.9e-48
Identity = 125/344 (36.34%), Postives = 172/344 (50.00%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARR        +P  +++ C++ D   +   LSPL  VTH+F++TWA   R   
Sbjct: 56  WKVYGVARRTRPAWHEDNP-INYVQCDISDPDDSQAKLSPLTDVTHVFYVTWAN--RSTE 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGG-GGGEVVYCEDSPR 127
           Q+  + N  M  N LDA++P+   L+H+SLQTG KHY     S G     +  Y ED PR
Sbjct: 116 QENCEANSKMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESYGKIESHDPPYTEDLPR 175

Query: 128 AESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKK 187
            + + NFYY LED++ E +   +  + WS+ RPG I G S  +  N +G+LCVY AICK 
Sbjct: 176 LKYM-NFYYDLEDIMLEEVE-KKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKH 235

Query: 188 LNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWG 247
                 F G  A W+  Y D SDA L AE H+WAA                    K  W 
Sbjct: 236 EGKVLRFTGCKAAWDG-YSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWK 295

Query: 248 AVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDS 307
            +AE+ GV  G  +E +   +      M  K  VW EIV E GL  TK+ ++  W F D 
Sbjct: 296 VLAEQFGVGCGEYEEGV---DLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDV 355

Query: 308 LFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           +    E  + S  KS   GF       ++ + WID  +  K++P
Sbjct: 356 ILG-NECFLDSMNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-48
Identity = 125/343 (36.44%), Postives = 168/343 (48.98%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP   S   D    +ISC++LD       LSPL  VTH+F+ TW    R   
Sbjct: 56  WKVYGVARRP-RPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTK--RSTE 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA 127
           ++  + N  ML N L+A++P+   L+H+ LQTG KHY            +    ED PR 
Sbjct: 116 KENCEANGKMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDWKIKRHDPPLTEDLPRL 175

Query: 128 ESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL 187
           +S  NFYY  ED+L E +   +  + WS+ RPG I G S  +  N +G+LCVY AICK  
Sbjct: 176 DS-QNFYYTQEDILFEEVQ-KKESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHE 235

Query: 188 NMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWGA 247
                F G    W+  Y D SDA L AE  +WAA                    K  W  
Sbjct: 236 GAVLRFPGCKGAWDG-YSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKV 295

Query: 248 VAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSL 307
           +AE+ GV  G  +E     E      M +KG VW +IV E GL  TK+ ++ NW F D +
Sbjct: 296 LAEQFGVECGEYEE---GKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIV 355

Query: 308 FRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
               E  + +  KS   GF       +S + WID ++  K++P
Sbjct: 356 LG-NECWLDTMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.2e-48
Identity = 123/345 (35.65%), Postives = 172/345 (49.86%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP   S   D   ++ISC++ +       L PL  VTH+F+ TW +  R   
Sbjct: 57  WKVYGVARRP-RPSWNEDHPINYISCDVSNTAEVEAKLPPLSDVTHIFYATWTS--RSSE 116

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHY-ASLTSSTGGGGGEVVYCEDSPR 127
           ++  + N  ML N LD ++P+   L+H+ LQTG  HY AS+      G  +    ED PR
Sbjct: 117 EENCEANGKMLKNVLDTMIPNCPNLKHICLQTGRFHYVASVVDWKINGSHDTPLTEDLPR 176

Query: 128 AESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKK 187
             + NNFYY  ED+L E +   +  + WS+ RPG I G S  +  N +G+LCVY AICK+
Sbjct: 177 L-NTNNFYYTQEDILLEEVK-RKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKQ 236

Query: 188 LNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWG 247
                 F G    W+  Y D +DA L AE ++WAA                    K +W 
Sbjct: 237 EGAVLRFPGCKGAWDG-YSDCADADLIAEHYIWAALDPHAKNQSFNVSNGDVFKWKHLWK 296

Query: 248 AVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDS 307
            +AE+ GV  G   E     +      M +KG VW +IV E GL  TK+ ++  W F D+
Sbjct: 297 VLAEQFGVECGGY-EYEEGQQVRLQDVMKDKGPVWDKIVRENGLSNTKLEDVGKWWFSDT 356

Query: 308 LFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 333
           +    E  + S  KS   GF       +  LYWI  ++  K++PS
Sbjct: 357 IL-WNECRLDSMNKSKEHGFLGFRNSKNCFLYWIHKLKAYKIVPS 393

[Close]

BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: XP_023519075.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 671 bits (1732), Expect = 1.34e-242
Identity = 333/354 (94.07%), Postives = 333/354 (94.07%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA
Sbjct: 34  MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY
Sbjct: 94  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
           CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY
Sbjct: 154 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA                
Sbjct: 214 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKAVEINDGEAFNVCNGWS 273

Query: 241 -----IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGEL 300
                IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGEL
Sbjct: 274 FTWKEIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGEL 333

Query: 301 ANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           ANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 ANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 387

[Close]

BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: XP_022970466.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 642 bits (1657), Expect = 4.00e-231
Identity = 320/357 (89.64%), Postives = 324/357 (90.76%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           MLLSTAGWKVYGVARRPDNISPISDPNFHFISC+LLDRRSAHLNLSP+RHVTHMFWITWA
Sbjct: 34  MLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPVRHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCSDQNKAMLANALDAILPSA ALRHVSLQTGVKHYASLTSST  GGGEVVY
Sbjct: 94  AQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSLQTGVKHYASLTSSTSSGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
            EDSPRAES NNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY
Sbjct: 154 SEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT+A                
Sbjct: 214 GAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATQAVEINAITDREAFNVCN 273

Query: 241 --------IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 300
                   IWGAVAEKLGVT GAEDERMFSPEFHYT AMAEKG VWAEIVEEEGLVAT+M
Sbjct: 274 GWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEM 333

Query: 301 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: KAG7019139.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641 bits (1654), Expect = 1.15e-230
Identity = 321/357 (89.92%), Postives = 324/357 (90.76%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           +LLSTAGWKVYGVARRPDNISPISDPNFHFISC+LLDRRSAHLNLSPL HVTHMFWITWA
Sbjct: 34  LLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPLCHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCSDQNKAMLANALDAILPSA ALRHVSLQTGVKHYASLT STGGGGGEVVY
Sbjct: 94  AQLRFDSQDCSDQNKAMLANALDAILPSAPALRHVSLQTGVKHYASLTGSTGGGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
            EDSPRAES NNFYYVLEDLLRERLSG RRVVAWSILRPGLILGCSNRTFFNFMGSLCVY
Sbjct: 154 SEDSPRAESGNNFYYVLEDLLRERLSGRRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQHVWA TKA                
Sbjct: 214 GAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHVWATTKAVEINAITYGEAFNVCN 273

Query: 241 --------IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 300
                   IWGAVAEKLGVTAGAEDERMFSPEF YTAAMAEKGTVWAEIVEEEGLVAT+M
Sbjct: 274 GWSFTWKEIWGAVAEKLGVTAGAEDERMFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEM 333

Query: 301 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: KAG6583366.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 638 bits (1646), Expect = 1.90e-229
Identity = 319/357 (89.36%), Postives = 324/357 (90.76%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           +LLSTAGWKVYGVARRPDNISPISDPNFHFISC+LLDRRSAHLNLSPL HVTHMFWITWA
Sbjct: 34  LLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPLCHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCS+QNKAMLANALDAILPSA ALRHVSLQTGVKHYASLT STGGGGGEVVY
Sbjct: 94  AQLRFDSQDCSNQNKAMLANALDAILPSAPALRHVSLQTGVKHYASLTGSTGGGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
            EDSPRAES NNFYYVLEDLLRERLSG RRVVAWSILRPGLILGCSNRTFFNFMGS+CVY
Sbjct: 154 SEDSPRAESGNNFYYVLEDLLRERLSGRRRVVAWSILRPGLILGCSNRTFFNFMGSVCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKL MPFVFGGT ACWEEVYIDGSDARLTAEQHVWAATKA                
Sbjct: 214 GAICKKLKMPFVFGGTGACWEEVYIDGSDARLTAEQHVWAATKAVEINAITDGEAFNVCN 273

Query: 241 --------IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 300
                   IWGAVAEKLGVTAGAEDERMFSPEF YTAAMAEKGTVWAEIVEEEGLVAT+M
Sbjct: 274 GWSFTWKEIWGAVAEKLGVTAGAEDERMFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEM 333

Query: 301 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: XP_022964858.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 638 bits (1645), Expect = 2.69e-229
Identity = 320/357 (89.64%), Postives = 323/357 (90.48%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           MLLSTAGWKVYGVARRPDNISPISDPNFHFISC+LLDRRSAHLNLSPL HVTHMFWITWA
Sbjct: 34  MLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPLGHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCSDQNKAMLANALDAILPSA ALRHVSLQTGVKHYASLTSSTG GGGEVVY
Sbjct: 94  AQLRFDSQDCSDQNKAMLANALDAILPSAPALRHVSLQTGVKHYASLTSSTGSGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
            E+SPRAES NNFYYVLEDLLRERLSG RRVVAWSILRPGLILGCSNRTFFNFMGSLCVY
Sbjct: 154 SENSPRAESGNNFYYVLEDLLRERLSGRRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKL MPFVFGGTAACWEEVYID SDARLTAEQHVWAATKA                
Sbjct: 214 GAICKKLKMPFVFGGTAACWEEVYIDSSDARLTAEQHVWAATKAVEINALTDGEAFNVCN 273

Query: 241 --------IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 300
                   IWGAVAEKLGVT GAEDERMFSPEF YTAAMAEKGTVWAEIVEEEGLVAT+M
Sbjct: 274 GWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEM 333

Query: 301 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 1.94e-231
Identity = 320/357 (89.64%), Postives = 324/357 (90.76%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           MLLSTAGWKVYGVARRPDNISPISDPNFHFISC+LLDRRSAHLNLSP+RHVTHMFWITWA
Sbjct: 34  MLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPVRHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCSDQNKAMLANALDAILPSA ALRHVSLQTGVKHYASLTSST  GGGEVVY
Sbjct: 94  AQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSLQTGVKHYASLTSSTSSGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
            EDSPRAES NNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY
Sbjct: 154 SEDSPRAESGNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT+A                
Sbjct: 214 GAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATQAVEINAITDREAFNVCN 273

Query: 241 --------IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 300
                   IWGAVAEKLGVT GAEDERMFSPEFHYT AMAEKG VWAEIVEEEGLVAT+M
Sbjct: 274 GWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEM 333

Query: 301 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 1.30e-229
Identity = 320/357 (89.64%), Postives = 323/357 (90.48%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWA 60
           MLLSTAGWKVYGVARRPDNISPISDPNFHFISC+LLDRRSAHLNLSPL HVTHMFWITWA
Sbjct: 34  MLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPLGHVTHMFWITWA 93

Query: 61  AQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVY 120
           AQLRFDSQDCSDQNKAMLANALDAILPSA ALRHVSLQTGVKHYASLTSSTG GGGEVVY
Sbjct: 94  AQLRFDSQDCSDQNKAMLANALDAILPSAPALRHVSLQTGVKHYASLTSSTGSGGGEVVY 153

Query: 121 CEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 180
            E+SPRAES NNFYYVLEDLLRERLSG RRVVAWSILRPGLILGCSNRTFFNFMGSLCVY
Sbjct: 154 SENSPRAESGNNFYYVLEDLLRERLSGRRRVVAWSILRPGLILGCSNRTFFNFMGSLCVY 213

Query: 181 GAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA---------------- 240
           GAICKKL MPFVFGGTAACWEEVYID SDARLTAEQHVWAATKA                
Sbjct: 214 GAICKKLKMPFVFGGTAACWEEVYIDSSDARLTAEQHVWAATKAVEINALTDGEAFNVCN 273

Query: 241 --------IWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 300
                   IWGAVAEKLGVT GAEDERMFSPEF YTAAMAEKGTVWAEIVEEEGLVAT+M
Sbjct: 274 GWSFTWKEIWGAVAEKLGVTVGAEDERMFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEM 333

Query: 301 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 333
           GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Sbjct: 334 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 575 bits (1483), Expect = 7.66e-205
Identity = 293/362 (80.94%), Postives = 309/362 (85.36%), Query Frame = 0


Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDRRS   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLT---SSTGGGG-GE 121
           QLR DS DCSDQN+AMLANALDAILPSA ALRHVSLQTG+KHYASLT   +  GGGG GE
Sbjct: 95  QLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGE 154

Query: 122 VVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSL 181
           VVYCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILGCSNRTFFNFMGSL
Sbjct: 155 VVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSL 214

Query: 182 CVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA------------- 241
           CVYGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA             
Sbjct: 215 CVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHIWVATKAVEINATADGEAFN 274

Query: 242 -----------IWGAVAEKLGV-TAGAEDE--RMFSPEFHYTAAMAEKGTVWAEIVEEEG 301
                      IWGAVAEKLGV T GAE+E  RMFS EFHYTAAM +KG VWAEIVEEEG
Sbjct: 275 VCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEG 334

Query: 302 LVATKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLI 332
           LV T+M ELANWGFLD+LFRLPEKMVASRAKSDRLGF VKYKMLDS+LYWIDVMR+DKLI
Sbjct: 335 LVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLI 394

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy TrEMBL
Match: A0A1S3C5H7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=3 SV=1)

HSP 1 Score: 562 bits (1449), Expect = 9.24e-200
Identity = 287/356 (80.62%), Postives = 300/356 (84.27%), Query Frame = 0


Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDR+S   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGG--EVV 121
           QLR DS  CSDQN+AMLANALDAILPSA ALRHVSLQTGVKHYASLT    G GG  EVV
Sbjct: 95  QLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVV 154

Query: 122 YCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCV 181
           YCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILG SNRTFFNFMGSLCV
Sbjct: 155 YCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCV 214

Query: 182 YGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA--------------- 241
           YGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA               
Sbjct: 215 YGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGW 274

Query: 242 ------IWGAVAEKLGV--TAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 301
                 IWG VAEKLGV  TA  E ERMFS EFHYTAAM +KG VWAEIVEEEGLVAT+M
Sbjct: 275 SFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEM 334

Query: 302 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 332
            ELANW FLD+LFRLPEKMVASRAKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 335 EELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

[Close]

BLAST of Cp4.1LG20g08120 vs. ExPASy TrEMBL
Match: A0A5D3BGL7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001240 PE=3 SV=1)

HSP 1 Score: 561 bits (1446), Expect = 2.95e-199
Identity = 287/359 (79.94%), Postives = 300/359 (83.57%), Query Frame = 0


Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDR+S   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGG--EVV 121
           QLR DS  CSDQN+AMLANALDAILPSA ALRHVSLQTGVKHYASLT    G GG  EVV
Sbjct: 95  QLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVV 154

Query: 122 YCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCV 181
           YCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILG SNRTFFNFMGSLCV
Sbjct: 155 YCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCV 214

Query: 182 YGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA--------------- 241
           YGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA               
Sbjct: 215 YGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWVATKAVEINATADGEAFNVC 274

Query: 242 ---------IWGAVAEKLGV--TAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVA 301
                    IWG VAEKLGV  TA  E ERMFS EFHYTAAM +KG VWAEIVEEEGLVA
Sbjct: 275 NGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVA 334

Query: 302 TKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 332
           T+M ELANW FLD+LFRLPEKMVASRAKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 335 TEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]

BLAST of Cp4.1LG20g08120 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 354.0 bits (907), Expect = 1.3e-97
Identity = 182/356 (51.12%), Postives = 232/356 (65.17%), Query Frame = 0


Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRH-VTHMFWITW 60
           +L+S  GW++YGVAR P+  S      ++FISC+LL+       LSPL+  V+H+FW+TW
Sbjct: 36  LLMSKPGWRIYGVARNPEINS--MTKMYNFISCDLLNASETKQRLSPLQDIVSHVFWVTW 95

Query: 61  AAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSST---GGGGG 120
           + +   D+ +C  QNK ML NALDAILP+A  L+H SLQTG+KHY SL   T   G G  
Sbjct: 96  SGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQTGMKHYVSLVEETMARGEGSS 155

Query: 121 EVVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGS 180
              Y E+ PR  S  NFYYVLEDLL+E+++  R  V WS+ RPGL++G S+RT +NFMGS
Sbjct: 156 LYYYSEECPRKSSGKNFYYVLEDLLKEKIT--RSSVVWSVQRPGLLMGSSSRTLYNFMGS 215

Query: 181 LCVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------- 240
           LCVYGA+CK LN+PFVFGGT  CWEE YIDGSD+ L AEQH++AAT              
Sbjct: 216 LCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQHIFAATSGKVREKGEAFNAI 275

Query: 241 -------KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATK 300
                  K IW  + +KLGV     +  MF   F +   M E+  VW EIV +E LV T+
Sbjct: 276 NGVGFTWKEIWPEIGKKLGVQ--VNETTMFDEGFWFGREMVERKHVWDEIVVKEKLVRTE 335

Query: 301 MGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           + +LANW FLD+LFR P K++  R K DR GF  KY+ LDS+LYWIDVMR +KLIP
Sbjct: 336 IEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of Cp4.1LG20g08120 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 125/343 (36.44%), Postives = 172/343 (50.15%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP        P   +I C++ D       LSPL  VTH+F++TW  +   +S
Sbjct: 56  WKVYGVARRPRPTWNADHP-IDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNR-ESES 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA 127
           ++C + N +ML N L AI+P A  LRHV LQTG KHY    ++  G   +  + ED PR 
Sbjct: 116 ENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRL 175

Query: 128 ESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL 187
           + + NFYY  ED+L E +      V WSI RP +I G S  +  N +G+LCVY AICK  
Sbjct: 176 Q-IQNFYYTQEDILFEEIKKI-ETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHE 235

Query: 188 NMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWGA 247
             P +F G+   WE  ++  SDA L AEQ +WAA                    K +W  
Sbjct: 236 GSPLLFPGSKKAWEG-FMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKI 295

Query: 248 VAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSL 307
           +AE+ G+     +E            M  K  VW E+V+E  L   K+ E+  W F D +
Sbjct: 296 LAEQFGIEEYGFEE---GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVI 355

Query: 308 FRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
             + E M+ S  KS   GF       +S + WID  +  K++P
Sbjct: 356 LGV-EGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of Cp4.1LG20g08120 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 125/343 (36.44%), Postives = 172/343 (50.15%), Query Frame = 0


Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP        P   +I C++ D       LSPL  VTH+F++TW  +   +S
Sbjct: 55  WKVYGVARRPRPTWNADHP-IDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNR-ESES 114

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA 127
           ++C + N +ML N L AI+P A  LRHV LQTG KHY    ++  G   +  + ED PR 
Sbjct: 115 ENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRL 174

Query: 128 ESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL 187
           + + NFYY  ED+L E +      V WSI RP +I G S  +  N +G+LCVY AICK  
Sbjct: 175 Q-IQNFYYTQEDILFEEIKKI-ETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHE 234

Query: 188 NMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWGA 247
             P +F G+   WE  ++  SDA L AEQ +WAA                    K +W  
Sbjct: 235 GSPLLFPGSKKAWEG-FMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKI 294

Query: 248 VAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSL 307
           +AE+ G+     +E            M  K  VW E+V+E  L   K+ E+  W F D +
Sbjct: 295 LAEQFGIEEYGFEE---GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVI 354

Query: 308 FRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
             + E M+ S  KS   GF       +S + WID  +  K++P
Sbjct: 355 LGV-EGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g08120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1C9CX66	3.7e-52	38.37	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q9STX2	1.6e-50	36.44	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
Q6PQJ9	1.9e-48	36.34	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]
A0A221J5X3	4.2e-48	36.44	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
A0A221J5X1	4.2e-48	35.65	(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ...	[more]

BLAST of Cp4.1LG20g08120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519075.1	1.34e-242	94.07	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022970466.1	4.00e-231	89.64	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]
KAG7019139.1	1.15e-230	89.92	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]
KAG6583366.1	1.90e-229	89.36	(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022964858.1	2.69e-229	89.64	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]

BLAST of Cp4.1LG20g08120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I0N6	1.94e-231	89.64	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1HM54	1.30e-229	89.64	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0LVI2	7.66e-205	80.94	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P...	[more]
A0A1S3C5H7	9.24e-200	80.62	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=...	[more]
A0A5D3BGL7	2.95e-199	79.94	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo var. makuwa...	[more]

BLAST of Cp4.1LG20g08120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58750.1	1.3e-97	51.12	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	1.1e-51	36.44	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	1.1e-51	36.44	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 1..331 e-value: 1.3E-77 score: 263.2
None	No IPR available	PANTHER	PTHR32487:SF12	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1	coord: 223..331 coord: 2..224
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 223..331 coord: 2..224
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..227

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g08120.1	Cp4.1LG20g08120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity