Cp4.1LG20g07520 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g07520 vs. ExPASy Swiss-Prot
Match: Q96JG6 (Syndetin OS=Homo sapiens OX=9606 GN=VPS50 PE=1 SV=3)

HSP 1 Score: 107.8 bits (268), Expect = 6.2e-22
Identity = 184/826 (22.28%), Postives = 322/826 (38.98%), Query Frame = 0

Query: 98  RFGLSSSSEELS---SIYGSRIHGQE------------------VEELEDVFYEED-FDP 157
           R GL S  E LS   +I   R+ G+E                  +  +E V++  D FD 
Sbjct: 10  RQGLKSPQESLSDLGAIESLRVPGKEEFRELREQPSDPQAEQELINSIEQVYFSVDSFDI 69

Query: 158 VRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHEVMVKGMHLFRELEKD 217
           V++ LE +P    +L+  E    K   Q   V+++++  ++E     VK +     L+  
Sbjct: 70  VKYELEKLP-PVLNLQELEAYRDKLKQQQAAVSKKVADLILEKQPAYVKELERVTSLQTG 129

Query: 218 LKIANVICRNGKRHLNSSMLAVSR-DLIVNSNSKKKQALL-----------------LSS 277
           L++A VIC NG+RHLN +    ++  L + +N +K+Q L+                   S
Sbjct: 130 LQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLKSLRTIKTLQRTDVRLS 189

Query: 278 EYWTNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSE-LSVIREMSCG-- 337
           E    E   G+  +CL    A           ++ S+    L+   E L V     C   
Sbjct: 190 EMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQIEEQLDVALSKICKNF 249

Query: 338 -----IEVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKDIVQ---EDQDVHIQNSR 397
                 +V  AY L+G      +++   F Q + +     +   V+    + D   Q  +
Sbjct: 250 DINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGYVELCAGNTDTKFQ--K 309

Query: 398 LTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDSIDQTPNMKQQEDNY 457
           L Y DLC  +    +  CL      L+ +M SYY              +T    ++ DN 
Sbjct: 310 LQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYY--------------RTMEWHEKHDN- 369

Query: 458 DINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSGSPWYHLRKDAINFVS 517
                DT  ++   + +GT   TN D G                             ++ 
Sbjct: 370 ----EDTASASEGSNMIGTEE-TNFDRG-----------------------------YIK 429

Query: 518 QTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFILAGEAFCGVEAVEF 577
           + L+     +WQ    +V   L    +       F+   + ++  +  GE FCG ++   
Sbjct: 430 KKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIISRLMQVGEEFCGSKSEVL 489

Query: 578 RQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSFSGLVGDGAPLFVHCD 637
           ++ ++    NY+  +H+  +  L+M +E E W   P           +  + + L +H  
Sbjct: 490 QESIRKQSVNYFKNYHRTRLDELRMFLENETWELCP-----------VKSNFSILQLHEF 549

Query: 638 GNSSKGKAPRSDKSTSSVSTGMDR-SGFLRWLKNGNPFLLKQMHTCKEGIPNGELDGSAG 697
               + ++P    S   VST     + F ++   GNPF ++  H  +E       D  A 
Sbjct: 550 KFMEQSRSPSVSPSKQPVSTSSKTVTLFEQYCSGGNPFEIQANHKDEE-----TEDVLAS 609

Query: 698 EGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDS------QLPSRILKPRLSL 757
            G++        Y +  S+       D  E+L  D++DE +       +    LK R   
Sbjct: 610 NGYESDEQEKSAYQEYDSD------SDVPEELKRDYVDEQTGDGPVKSVSRETLKSRKKS 669

Query: 758 NHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQLFAVFFYFVYET 817
           ++S N  N  I   T ++L ++R + KY ++M  L+ +  +    M QLF  + Y +Y  
Sbjct: 670 DYSLNKVNAPIL--TNTTLNVIRLVGKYMQMMNILKPIAFDVIHFMSQLFDYYLYAIYTF 729

Query: 818 FGQLNT-TSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSAS-NSTFAFNEVTPS 862
           FG+ ++  S G G   S   +L+T L+R  +         S+ P+A+  +     E  PS
Sbjct: 730 FGRNDSLESTGLGLSSS---RLRTTLNRIQESLIDLE--VSADPTATLTAAEERKEKVPS 754

BLAST of Cp4.1LG20g07520 vs. ExPASy Swiss-Prot
Match: Q5ZKV9 (Syndetin OS=Gallus gallus OX=9031 GN=VPS50 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.8e-21
Identity = 181/790 (22.91%), Postives = 312/790 (39.49%), Query Frame = 0

Query: 121 VEELEDVFYEED-FDPVRHILEHVP---SEESDLEYFEKQATKRLAQLDKVAERLSRHLM 180
           +  +E+V++  D FD V++ LE +P   S +   EY +K   ++ A+L++V         
Sbjct: 54  INSIEEVYFSNDAFDIVKYELERLPPVLSLQELEEYRDKLKQQQAAELERVT-------- 113

Query: 181 EHHEVMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSR-DLIVNSNSKKKQALL- 240
                          L+  L++A VIC +G+RHLN +    ++  L + +N +K+Q L+ 
Sbjct: 114 --------------SLQNGLQLAAVICADGRRHLNIAKEGFTQTSLGLLANQRKRQLLIG 173

Query: 241 ----------------LSSEYWTNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQL 300
                             SE    E   G+  +CL    A           ++ S+    
Sbjct: 174 LLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDT 233

Query: 301 LDSFSE-LSVIREMSCG-------IEVVDAYALIGDVSGLAEKIQSFFMQEVISETHSAL 360
           L+   E L V     C         +V  AY L+G      +++   F Q + +     +
Sbjct: 234 LEQIEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVV 293

Query: 361 KDIVQ---EDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQL 420
              V+    + D   Q  +L Y DLC  I    +  CL      L+ +M SYY+      
Sbjct: 294 LGYVELCAGNTDTKFQ--KLQYKDLCTHITSDSYIPCLADLCKALWEVMLSYYRTM---- 353

Query: 421 DTKDSIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTV 480
                        Q  +N+D       +    VSSV       SDG          SS V
Sbjct: 354 -------------QWHENHD------QDEAATVSSV-------SDG----------SSVV 413

Query: 481 STSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYED 540
            T  + +        ++V + L+     +WQ    +V   L    + +     F+   + 
Sbjct: 414 GTEENSFDR------SYVKKKLEHGLSRIWQDVQLKVKTYLLGTDLSNFKYDDFIFVLDV 473

Query: 541 LNVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPP---- 600
           ++  +  GE FCG ++   ++ ++    NY+  +H+  +  L+M +E E W   P     
Sbjct: 474 ISRLMQVGEEFCGSKSEVLQESIRKQSVNYFKTYHRTRLEELRMFLENETWELCPVKSSF 533

Query: 601 DTVQVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPF 660
           D +Q+  F  +V   +P       + S  K P S  ST+          F ++   GNPF
Sbjct: 534 DILQLHEFKFMVQSRSP-------SVSPSKQPASAISTTVTL-------FEQYCNGGNPF 593

Query: 661 LLKQMHTCKEGIPNGELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFID 720
            ++      +   +   D  A  G++        Y +  S+       D  E+L  D++D
Sbjct: 594 EIQ-----ADSKDDETEDVLASNGYESDEQEKSAYQEYDSD------SDVPEELKRDYVD 653

Query: 721 EDS------QLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIV 780
           E +       +    L+ R   ++S N  N  I   T ++L ++R + KY ++M  L+ +
Sbjct: 654 EQTGDAPMKSVSRETLRSRKKSDYSLNKGNAPIL--TNTTLNVVRLVGKYMQMMNILKPI 713

Query: 781 NVEFFKGMCQLFAVFFYFVYETFGQLNT-TSGGKGFPDSLNYKLKTALSRAAQ---DCDQ 840
             +    M QLF  + Y VY  FG+ +T  S G G   S   +L+T L+R  +   D + 
Sbjct: 714 AFDVIHFMSQLFDYYLYAVYTFFGRNDTFESTGLGLSSS---RLRTTLNRIQESLIDLEV 739

Query: 841 WRRPHSSSPSASNSTFAFNEVTPSPPGSSLGYF-HGTSL-GLKERCAGADSLSLVARIMH 862
              P  +  +A        E  PSP  S L     G+SL GL ER    +SL  +A    
Sbjct: 774 SADPTGTLTAAEER----KEKVPSPHLSHLVVLTSGSSLYGLAERVVATESLVFLAEQFE 739

BLAST of Cp4.1LG20g07520 vs. ExPASy Swiss-Prot
Match: Q8CI71 (Syndetin OS=Mus musculus OX=10090 GN=Vps50 PE=1 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 1.2e-20
Identity = 176/785 (22.42%), Postives = 309/785 (39.36%), Query Frame = 0

Query: 121 VEELEDVFYEED-FDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHH 180
           +  +E V++  D FD V++ LE +P    +L+  E+   K   Q   V+++++  ++E  
Sbjct: 54  INSIEQVYFSADPFDIVKYELEKLP-PVLNLQELEEYRDKLKQQQSAVSKKVADLILEKQ 113

Query: 181 EVMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSR-DLIVNSNSKKKQAL----- 240
              VK +     L+  L++A VIC NG+RHLN +    ++  L + +N +K+Q L     
Sbjct: 114 PAYVKELERVTSLQTGLQLAAVICTNGRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLK 173

Query: 241 ------------LLSSEYWTNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDS 300
                       +  SE    E   G+  +CL    A           ++ S+    L+ 
Sbjct: 174 SLRTIKTLQRTDIRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQ 233

Query: 301 FSE-LSVIREMSCG-------IEVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKDI 360
             E L V     C         +V  AY L+G      +++   F Q + +     +   
Sbjct: 234 IEEQLDVALSKICKNFDINHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGY 293

Query: 361 VQ---EDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTK 420
           V+    + D   Q  +L Y DLC  +    +  CL      L+ +M SYY          
Sbjct: 294 VELCAGNTDTKFQ--KLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYY---------- 353

Query: 421 DSIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTS 480
               +T    ++ DN +                 TAA                S+ +ST 
Sbjct: 354 ----RTMEWHEKHDNEE-----------------TAAAAEG------------SNVMSTE 413

Query: 481 GSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNV 540
            + +         +V + L+     +WQ    +V   L    +       F+   + ++ 
Sbjct: 414 EATFDR------GYVKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVSR 473

Query: 541 FILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDT----V 600
            +  GE FCG ++   ++ ++    NY+   H+  +  L+M +E E W   P  +    +
Sbjct: 474 LMQVGEEFCGSKSEVLQESIRKQSVNYFKNHHRIRLDELRMFLENETWELCPVKSNFSIL 533

Query: 601 QVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLK 660
           Q+  F  L    +P       + S  K P    +TSS    +    F ++   GNPF ++
Sbjct: 534 QLHEFKFLEQSRSP-------SVSPSKQP---SATSSKPVTL----FEQYCSGGNPFEIQ 593

Query: 661 QMHTCKEGIPNGELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDS 720
             H  +E       D  A  G++        Y D  S+       D  E+L  D++DE +
Sbjct: 594 ADHKDEE-----TEDVLASNGYESDEQEKSAYQDYDSD------SDVPEELKRDYVDEQT 653

Query: 721 ------QLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVE 780
                  +    LK R   ++S N  N  I   T ++L ++R + KY ++M  L+ +  +
Sbjct: 654 GDVPVKSVSRETLKSRKKSDYSLNKVNAPIL--TNTTLNVIRLVGKYMQMMNILKPIAFD 713

Query: 781 FFKGMCQLFAVFFYFVYETFGQLNT-TSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHS 840
               M QLF  + Y +Y  FG+ ++  S G G   S   +LKT L+R  +         S
Sbjct: 714 VIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSS---RLKTTLNRIQESLIDLE--GS 754

Query: 841 SSPSAS-NSTFAFNEVTPSPPGSSLGYFHG--TSLGLKERCAGADSLSLVARIMHRSKAH 862
           + P+A+  +     E  PSP  + L       T  GL ER    +SL  +A      + H
Sbjct: 774 ADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAEQFEFLQPH 754

BLAST of Cp4.1LG20g07520 vs. ExPASy Swiss-Prot
Match: F1LSG8 (Syndetin OS=Rattus norvegicus OX=10116 GN=Vps50 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 2.2e-19
Identity = 174/785 (22.17%), Postives = 307/785 (39.11%), Query Frame = 0

Query: 121 VEELEDVFYEED-FDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHH 180
           +  +E V++  D FD V++ LE +P    +L+  E+   K   Q   V+++++  ++E  
Sbjct: 54  INSIEQVYFSADPFDIVKYELEKLP-PVLNLQELEEYRDKLKQQQAAVSKKVADLILEKQ 113

Query: 181 EVMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSR-DLIVNSNSKKKQALL---- 240
              VK +     L+  L++A VIC N +RHLN +    ++  L + +N +K+Q L+    
Sbjct: 114 PAYVKELERVTSLQTGLQLAAVICTNSRRHLNIAKEGFTQASLGLLANQRKRQLLIGLLK 173

Query: 241 -------------LSSEYWTNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDS 300
                          SE    E   G+  +CL    A           ++ S+    L+ 
Sbjct: 174 SLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSCISELNSKLQDTLEQ 233

Query: 301 FSE-LSVIREMSCG-------IEVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKDI 360
             E L V     C         +V  AY L+G      +++   F Q + +     +   
Sbjct: 234 IEEQLDVALSKICKNFDVNHYTKVQQAYRLLGKTQTAMDQLHMHFTQAIHNTVFQVVLGY 293

Query: 361 VQ---EDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTK 420
           V+    + D   Q  +L Y DLC  +    +  CL      L+ +M SYY+  + +   K
Sbjct: 294 VELCAGNTDTKFQ--KLQYKDLCTHVTPDSYIPCLADLCKALWEVMLSYYR--TMEWHEK 353

Query: 421 DSIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTS 480
              ++T    +       N+  T E             TN D G                
Sbjct: 354 HDSEETAAASEGS-----NVMSTEE-------------TNFDRG---------------- 413

Query: 481 GSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNV 540
                        +V + L+     +WQ    +V   L    +       F+   + ++ 
Sbjct: 414 -------------YVKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIVSR 473

Query: 541 FILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDT----V 600
            +  GE FCG ++   ++ ++    NY+   H+  +  L+M +E E W   P  +    +
Sbjct: 474 LMQVGEEFCGSKSEVLQESIRKQSINYFKNHHRIRLDELRMFLENETWELCPVKSNFSIL 533

Query: 601 QVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLK 660
           Q+  F  L    +P       + S  K P    +TSS    +    F ++   GNPF ++
Sbjct: 534 QLHEFKFLEQSRSP-------SVSPSKQP---SATSSKPVTL----FEQYCSGGNPFEIQ 593

Query: 661 QMHTCKEGIPNGELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDS 720
             H  +E       D  A  G++        Y D  S+       D  E+L  D++DE +
Sbjct: 594 ADHKDEE-----TEDVLASNGYESDEQEKSAYQDYDSD------SDVPEELKRDYVDEQT 653

Query: 721 ------QLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVE 780
                  +    LK R   ++S N  N  I   T ++L ++R + KY ++M  L+ +  +
Sbjct: 654 GDVPVKSVSRETLKSRKKSDYSLNKVNAPIL--TNTTLNVIRLVGKYMQMMNILKPIAFD 713

Query: 781 FFKGMCQLFAVFFYFVYETFGQLNT-TSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHS 840
               M QLF  + Y +Y  FG+ ++  S G G   S   +L+T L+R  +         S
Sbjct: 714 VIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSS---RLRTTLNRIQESLIDLE--GS 754

Query: 841 SSPSAS-NSTFAFNEVTPSPPGSSLGYFHG--TSLGLKERCAGADSLSLVARIMHRSKAH 862
           + P+A+  +     E  PSP  + L       T  GL ER    +SL  +A      + H
Sbjct: 774 ADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLAEQFEFLQPH 754

BLAST of Cp4.1LG20g07520 vs. NCBI nr
Match: XP_023519395.1 (syndetin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1632 bits (4225), Expect = 0.0
Identity = 863/929 (92.90%), Postives = 869/929 (93.54%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE
Sbjct: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE
Sbjct: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLDMLPIL 240

Query: 241 TNEHVRGSNDV-CLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE--- 300
           +   +R + D+  +L  L    N    +AFQVLSEYLQLLDSFSELSVIREMSCGIE   
Sbjct: 241 SE--LRHAVDMQSMLEILVEEGNYS--KAFQVLSEYLQLLDSFSELSVIREMSCGIETWL 300

Query: 301 -------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360
                                    VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK
Sbjct: 301 GRTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360

Query: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420
           DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD
Sbjct: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420

Query: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSG 480
           SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSG
Sbjct: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSG 480

Query: 481 SPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVF 540
           SPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVF
Sbjct: 481 SPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVF 540

Query: 541 ILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSF 600
           ILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSF
Sbjct: 541 ILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSF 600

Query: 601 SGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQMHTC 660
           SGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQMHTC
Sbjct: 601 SGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQMHTC 660

Query: 661 KEGIPNGELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDSQLPSR 720
           KEGIPNGELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDSQLPSR
Sbjct: 661 KEGIPNGELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDSQLPSR 720

Query: 721 ILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQLFAV 780
           ILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQLFAV
Sbjct: 721 ILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQLFAV 780

Query: 781 FFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSASNSTFAF 840
           FFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSASNSTFAF
Sbjct: 781 FFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSASNSTFAF 840

Query: 841 NEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA---- 891
           NEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA    
Sbjct: 841 NEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVAVIED 900

BLAST of Cp4.1LG20g07520 vs. NCBI nr
Match: XP_022964722.1 (syndetin-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1570 bits (4066), Expect = 0.0
Identity = 836/932 (89.70%), Postives = 847/932 (90.88%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLLHTTSDRPEVPARAVAAAAVARALA LPPHQRFGLSSSSEELSSIYGSRIHGQE
Sbjct: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAALPPHQRFGLSSSSEELSSIYGSRIHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELEDVFYEEDFDPVRHILEHVPSEESDLEY EKQATKRLAQLDKVAERLSRHLMEHHE
Sbjct: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYLEKQATKRLAQLDKVAERLSRHLMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLDMLPIL 240

Query: 241 TNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE---- 300
           +  H    +   +L  L    N    +AFQVLSEYLQLLDSFSELSVIREMSCGIE    
Sbjct: 241 SELH-HAVDMQSMLEILVEEGNYS--KAFQVLSEYLQLLDSFSELSVIREMSCGIETWLG 300

Query: 301 ------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKD 360
                                   VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKD
Sbjct: 301 RTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKD 360

Query: 361 IVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDS 420
           IVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDS
Sbjct: 361 IVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDS 420

Query: 421 IDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSGS 480
           IDQTPNMKQQEDN DINL DTPESTINVSS+GTAAITNSDGGDLNRGSPTDSST STSGS
Sbjct: 421 IDQTPNMKQQEDNSDINLGDTPESTINVSSMGTAAITNSDGGDLNRGSPTDSSTASTSGS 480

Query: 481 PWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFI 540
           PWYHLRKDAINFVS+TLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFI
Sbjct: 481 PWYHLRKDAINFVSRTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFI 540

Query: 541 LAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSFS 600
           LAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTV+VVSF+
Sbjct: 541 LAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVEVVSFA 600

Query: 601 GLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQMHTCK 660
           GLVGDGAPLFVH DGNS KGK PRS      VSTGMDRSGFLRWLKNGNPFLLKQMHTCK
Sbjct: 601 GLVGDGAPLFVHSDGNSPKGKVPRS------VSTGMDRSGFLRWLKNGNPFLLKQMHTCK 660

Query: 661 EGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDSQL 720
           EGIPNG    E+DGSAGEGF RSNSSPMKYTDKLSNGANTV EDE+EDLLADFIDEDSQL
Sbjct: 661 EGIPNGALYGEMDGSAGEGFHRSNSSPMKYTDKLSNGANTVSEDENEDLLADFIDEDSQL 720

Query: 721 PSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQL 780
           PSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLE+VNVEFFKGMCQL
Sbjct: 721 PSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEMVNVEFFKGMCQL 780

Query: 781 FAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSASNST 840
           F VFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQW RPHSSSPSASNST
Sbjct: 781 FEVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSSPSASNST 840

Query: 841 FAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA- 891
           FAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA 
Sbjct: 841 FAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVAV 900

BLAST of Cp4.1LG20g07520 vs. NCBI nr
Match: KAG6583602.1 (Syndetin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1565 bits (4053), Expect = 0.0
Identity = 836/933 (89.60%), Postives = 848/933 (90.89%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLLHTTSDRPEVPARAVAAAAVARALA LPPHQRFGLSSSSEELSSIYGSRIHGQE
Sbjct: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAALPPHQRFGLSSSSEELSSIYGSRIHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELEDVFYEEDFDPVRHILEHVPSEESDLEY EKQATKRLAQLDKVAERLSRHLMEHHE
Sbjct: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYLEKQATKRLAQLDKVAERLSRHLMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLDMLPIL 240

Query: 241 TNEHVRGSNDV-CLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE--- 300
           +   +R + D+  +L  L    N    +AFQVLSEYLQLLDSFSELSVIREMSCGIE   
Sbjct: 241 SE--LRHAVDMQSMLEILVEEGNYS--KAFQVLSEYLQLLDSFSELSVIREMSCGIETWL 300

Query: 301 -------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360
                                    VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK
Sbjct: 301 GRTLQKLDSLLVEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360

Query: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420
           DIVQEDQDVHIQNSRL YSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD
Sbjct: 361 DIVQEDQDVHIQNSRLAYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420

Query: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSG 480
           SIDQTPNMKQQEDN DINL +TPESTINVSS+GTAAITNSDGGDLNRGSPTDSST STSG
Sbjct: 421 SIDQTPNMKQQEDNSDINLGETPESTINVSSMGTAAITNSDGGDLNRGSPTDSSTASTSG 480

Query: 481 SPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVF 540
           SPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVF
Sbjct: 481 SPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVF 540

Query: 541 ILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSF 600
           ILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTV+VVSF
Sbjct: 541 ILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVEVVSF 600

Query: 601 SGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQMHTC 660
           +GLVGDGAPLFVH DGNS KGK PRS      VSTGMDRSGFLRWLKNGNPFLLKQMHTC
Sbjct: 601 AGLVGDGAPLFVHSDGNSPKGKVPRS------VSTGMDRSGFLRWLKNGNPFLLKQMHTC 660

Query: 661 KEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDSQ 720
           KEGIPNG    E+DGSAGEGF RSNSSPMKYTDKLSNGANTV EDE+EDLLADFIDEDSQ
Sbjct: 661 KEGIPNGALYGEMDGSAGEGFHRSNSSPMKYTDKLSNGANTVSEDENEDLLADFIDEDSQ 720

Query: 721 LPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQ 780
           LPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQ
Sbjct: 721 LPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQ 780

Query: 781 LFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSASNS 840
           LF VFFYFVYETFGQLNTTSG KGFPDSLNYKLKTALSRAAQDCDQW RPHSSSPSASNS
Sbjct: 781 LFEVFFYFVYETFGQLNTTSGEKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSSPSASNS 840

Query: 841 TFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA 891
           TFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA
Sbjct: 841 TFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA 900

BLAST of Cp4.1LG20g07520 vs. NCBI nr
Match: XP_022970284.1 (syndetin-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1563 bits (4048), Expect = 0.0
Identity = 836/936 (89.32%), Postives = 851/936 (90.92%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AF+GDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFEGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE
Sbjct: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VE+LEDVFYEEDFDPVRHILEHVPSEESDLEY EKQATKRLAQLDKVAERLSRHLMEHHE
Sbjct: 121 VEDLEDVFYEEDFDPVRHILEHVPSEESDLEYLEKQATKRLAQLDKVAERLSRHLMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLDMLPIL 240

Query: 241 TNEHVRGSNDV-CLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE--- 300
           +   +R + D+  +L  L    N    +AFQVLSEYLQLLDSFSELSVIREMSCGIE   
Sbjct: 241 SE--LRHAVDMQSMLEILVEEGNYS--KAFQVLSEYLQLLDSFSELSVIREMSCGIETWL 300

Query: 301 -------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360
                                    VVDAYALIGDVSGLAEKIQSFFMQEVISETHS+LK
Sbjct: 301 GRTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSSLK 360

Query: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420
            IVQEDQDVHIQNSRLTYSDLCF+IPESKFRLCLLKTLAVLFTL+CSYYQIFSFQLDTKD
Sbjct: 361 GIVQEDQDVHIQNSRLTYSDLCFQIPESKFRLCLLKTLAVLFTLVCSYYQIFSFQLDTKD 420

Query: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITN---SDGGDLNRGSPTDSSTVS 480
           SIDQTPNMKQQEDN DINL DTPESTINVSS+GTAA TN    D  DLNRGSPTDSST S
Sbjct: 421 SIDQTPNMKQQEDNSDINLGDTPESTINVSSMGTAADTNFVYMDRSDLNRGSPTDSSTAS 480

Query: 481 TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540
           TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL
Sbjct: 481 TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540

Query: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600
           NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV
Sbjct: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600

Query: 601 VSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQM 660
           VSF GLVGDGAPLFVH DGNSSKGK PRSDK+TSSVSTGMDRSGFLRWLKNGNPFLLKQM
Sbjct: 601 VSFPGLVGDGAPLFVHSDGNSSKGKVPRSDKTTSSVSTGMDRSGFLRWLKNGNPFLLKQM 660

Query: 661 HTCKEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDE 720
           HTCKEGIPNG    ELDGSAGEGF RSNSSPMKYTDKLSNGANTV EDE+EDLLADFIDE
Sbjct: 661 HTCKEGIPNGALYGELDGSAGEGFHRSNSSPMKYTDKLSNGANTVSEDENEDLLADFIDE 720

Query: 721 DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG 780
           DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYA LMQKLEIVNVEFFKG
Sbjct: 721 DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYATLMQKLEIVNVEFFKG 780

Query: 781 MCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSA 840
           MCQLF VFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQW RPHSSSPSA
Sbjct: 781 MCQLFEVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSSPSA 840

Query: 841 SNSTFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT 891
           SNSTFAFNEVTPSPPG+SLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT
Sbjct: 841 SNSTFAFNEVTPSPPGNSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT 900

BLAST of Cp4.1LG20g07520 vs. NCBI nr
Match: XP_038895533.1 (syndetin isoform X1 [Benincasa hispida])

HSP 1 Score: 1449 bits (3751), Expect = 0.0
Identity = 780/936 (83.33%), Postives = 818/936 (87.39%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AFDGDLSEGFETSRFLFFVPF LLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFDGDLSEGFETSRFLFFVPFLLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLL TTSDRPEVPARAVAAAAVAR LA LPPHQRF LSSSSEELSSIYGSR HG E
Sbjct: 61  ARSLGLLPTTSDRPEVPARAVAAAAVARVLAGLPPHQRFSLSSSSEELSSIYGSRNHGHE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELE+VFYEEDFDPVRH+LEHVPSEE+DLEY EKQATKRLAQLDKVAERLS H+MEHHE
Sbjct: 121 VEELEEVFYEEDFDPVRHVLEHVPSEENDLEYLEKQATKRLAQLDKVAERLSHHVMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHL RELEKDLKIANVICRNGKRHLNSSML VSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLVRELEKDLKIANVICRNGKRHLNSSMLEVSRDLIVNSNSKKKQALLDMLPVL 240

Query: 241 TNEHVRGSNDV-CLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE--- 300
           +   +R + D+  +L  L    N    +AFQVLSEYLQLLDSFSELSVI+EMS G+E   
Sbjct: 241 SE--LRHAVDMQSMLETLVEEGNY--YKAFQVLSEYLQLLDSFSELSVIQEMSRGVEIWL 300

Query: 301 -------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360
                                    VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK
Sbjct: 301 GRTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360

Query: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420
           DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQI SFQLDTKD
Sbjct: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQILSFQLDTKD 420

Query: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNS---DGGDLNRGSPTDSSTVS 480
           SI+QT +MKQ EDN+DINL DT E TINVSS+G+  ITNS   DGGD NR S TDSST S
Sbjct: 421 SIEQTRSMKQPEDNHDINLGDTEELTINVSSMGSTGITNSVYMDGGDSNRESRTDSSTAS 480

Query: 481 TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540
           TSGSPWYHLRKDAI+FVSQTLQR RKNLWQL+TSRVSVLLSSAAVCSTSIHQFLKNYEDL
Sbjct: 481 TSGSPWYHLRKDAIHFVSQTLQRGRKNLWQLTTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540

Query: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600
           NVFILAGEAFCGVEAVEFRQKLK VCENYYVAFHKQSMHALKMVMEKENWLTLP DTVQV
Sbjct: 541 NVFILAGEAFCGVEAVEFRQKLKTVCENYYVAFHKQSMHALKMVMEKENWLTLPSDTVQV 600

Query: 601 VSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQM 660
           VSF+GL+GDGAPLFV  +GNSS GK PRSDKSTSS+STGMDRSGFL+WLK+GNPFLLK M
Sbjct: 601 VSFAGLLGDGAPLFVTSEGNSSNGKVPRSDKSTSSISTGMDRSGFLQWLKSGNPFLLKLM 660

Query: 661 HTCKEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDE 720
           HTCKEG PNG    E+DGS GE   RSN+SP KY D L+NGANTV EDE EDLLADFIDE
Sbjct: 661 HTCKEGTPNGIHYGEVDGSVGESPHRSNASPTKYPDNLANGANTVSEDEDEDLLADFIDE 720

Query: 721 DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG 780
           DSQLPSRI KP+LS NHSSNH+ND+ITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG
Sbjct: 721 DSQLPSRISKPKLSRNHSSNHSNDDITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG 780

Query: 781 MCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSA 840
           MCQLF VFFYFVYETFGQ +T+SGGKGFPDSLNYKLKTALSRAAQDC+QW RPHSSSPSA
Sbjct: 781 MCQLFEVFFYFVYETFGQPSTSSGGKGFPDSLNYKLKTALSRAAQDCEQWIRPHSSSPSA 840

Query: 841 SNSTFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT 891
           S+STF+FNEVTPSPPG+SLGY HGTS GLKER AGADSLSLVARIMHRSKAHIQSMLLQT
Sbjct: 841 SSSTFSFNEVTPSPPGTSLGYLHGTSFGLKERSAGADSLSLVARIMHRSKAHIQSMLLQT 900

BLAST of Cp4.1LG20g07520 vs. ExPASy TrEMBL
Match: A0A6J1HII5 (syndetin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464715 PE=4 SV=1)

HSP 1 Score: 1570 bits (4066), Expect = 0.0
Identity = 836/932 (89.70%), Postives = 847/932 (90.88%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLLHTTSDRPEVPARAVAAAAVARALA LPPHQRFGLSSSSEELSSIYGSRIHGQE
Sbjct: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAALPPHQRFGLSSSSEELSSIYGSRIHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELEDVFYEEDFDPVRHILEHVPSEESDLEY EKQATKRLAQLDKVAERLSRHLMEHHE
Sbjct: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYLEKQATKRLAQLDKVAERLSRHLMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLDMLPIL 240

Query: 241 TNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE---- 300
           +  H    +   +L  L    N    +AFQVLSEYLQLLDSFSELSVIREMSCGIE    
Sbjct: 241 SELH-HAVDMQSMLEILVEEGNYS--KAFQVLSEYLQLLDSFSELSVIREMSCGIETWLG 300

Query: 301 ------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKD 360
                                   VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKD
Sbjct: 301 RTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALKD 360

Query: 361 IVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDS 420
           IVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDS
Sbjct: 361 IVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKDS 420

Query: 421 IDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNSDGGDLNRGSPTDSSTVSTSGS 480
           IDQTPNMKQQEDN DINL DTPESTINVSS+GTAAITNSDGGDLNRGSPTDSST STSGS
Sbjct: 421 IDQTPNMKQQEDNSDINLGDTPESTINVSSMGTAAITNSDGGDLNRGSPTDSSTASTSGS 480

Query: 481 PWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFI 540
           PWYHLRKDAINFVS+TLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFI
Sbjct: 481 PWYHLRKDAINFVSRTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDLNVFI 540

Query: 541 LAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQVVSFS 600
           LAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTV+VVSF+
Sbjct: 541 LAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVEVVSFA 600

Query: 601 GLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQMHTCK 660
           GLVGDGAPLFVH DGNS KGK PRS      VSTGMDRSGFLRWLKNGNPFLLKQMHTCK
Sbjct: 601 GLVGDGAPLFVHSDGNSPKGKVPRS------VSTGMDRSGFLRWLKNGNPFLLKQMHTCK 660

Query: 661 EGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDEDSQL 720
           EGIPNG    E+DGSAGEGF RSNSSPMKYTDKLSNGANTV EDE+EDLLADFIDEDSQL
Sbjct: 661 EGIPNGALYGEMDGSAGEGFHRSNSSPMKYTDKLSNGANTVSEDENEDLLADFIDEDSQL 720

Query: 721 PSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKGMCQL 780
           PSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLE+VNVEFFKGMCQL
Sbjct: 721 PSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEMVNVEFFKGMCQL 780

Query: 781 FAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSASNST 840
           F VFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQW RPHSSSPSASNST
Sbjct: 781 FEVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSSPSASNST 840

Query: 841 FAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA- 891
           FAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVA 
Sbjct: 841 FAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQTNVAV 900

BLAST of Cp4.1LG20g07520 vs. ExPASy TrEMBL
Match: A0A6J1I532 (syndetin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469296 PE=4 SV=1)

HSP 1 Score: 1563 bits (4048), Expect = 0.0
Identity = 836/936 (89.32%), Postives = 851/936 (90.92%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AF+GDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFEGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE
Sbjct: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VE+LEDVFYEEDFDPVRHILEHVPSEESDLEY EKQATKRLAQLDKVAERLSRHLMEHHE
Sbjct: 121 VEDLEDVFYEEDFDPVRHILEHVPSEESDLEYLEKQATKRLAQLDKVAERLSRHLMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLDMLPIL 240

Query: 241 TNEHVRGSNDV-CLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE--- 300
           +   +R + D+  +L  L    N    +AFQVLSEYLQLLDSFSELSVIREMSCGIE   
Sbjct: 241 SE--LRHAVDMQSMLEILVEEGNYS--KAFQVLSEYLQLLDSFSELSVIREMSCGIETWL 300

Query: 301 -------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360
                                    VVDAYALIGDVSGLAEKIQSFFMQEVISETHS+LK
Sbjct: 301 GRTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSSLK 360

Query: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420
            IVQEDQDVHIQNSRLTYSDLCF+IPESKFRLCLLKTLAVLFTL+CSYYQIFSFQLDTKD
Sbjct: 361 GIVQEDQDVHIQNSRLTYSDLCFQIPESKFRLCLLKTLAVLFTLVCSYYQIFSFQLDTKD 420

Query: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITN---SDGGDLNRGSPTDSSTVS 480
           SIDQTPNMKQQEDN DINL DTPESTINVSS+GTAA TN    D  DLNRGSPTDSST S
Sbjct: 421 SIDQTPNMKQQEDNSDINLGDTPESTINVSSMGTAADTNFVYMDRSDLNRGSPTDSSTAS 480

Query: 481 TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540
           TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL
Sbjct: 481 TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540

Query: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600
           NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV
Sbjct: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600

Query: 601 VSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQM 660
           VSF GLVGDGAPLFVH DGNSSKGK PRSDK+TSSVSTGMDRSGFLRWLKNGNPFLLKQM
Sbjct: 601 VSFPGLVGDGAPLFVHSDGNSSKGKVPRSDKTTSSVSTGMDRSGFLRWLKNGNPFLLKQM 660

Query: 661 HTCKEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDE 720
           HTCKEGIPNG    ELDGSAGEGF RSNSSPMKYTDKLSNGANTV EDE+EDLLADFIDE
Sbjct: 661 HTCKEGIPNGALYGELDGSAGEGFHRSNSSPMKYTDKLSNGANTVSEDENEDLLADFIDE 720

Query: 721 DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG 780
           DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYA LMQKLEIVNVEFFKG
Sbjct: 721 DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYATLMQKLEIVNVEFFKG 780

Query: 781 MCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSA 840
           MCQLF VFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQW RPHSSSPSA
Sbjct: 781 MCQLFEVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSSPSA 840

Query: 841 SNSTFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT 891
           SNSTFAFNEVTPSPPG+SLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT
Sbjct: 841 SNSTFAFNEVTPSPPGNSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT 900

BLAST of Cp4.1LG20g07520 vs. ExPASy TrEMBL
Match: A0A1S3CG39 (syndetin OS=Cucumis melo OX=3656 GN=LOC103500532 PE=4 SV=1)

HSP 1 Score: 1431 bits (3705), Expect = 0.0
Identity = 771/936 (82.37%), Postives = 814/936 (86.97%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGS LGNP AFDGDLSEGFETSRFLFFVPF LLQGGGMDLSKVGEKILSSVRS
Sbjct: 1   MQPNLFPFGSALGNPLAFDGDLSEGFETSRFLFFVPFLLLQGGGMDLSKVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLL TTSDRPEVPARAVAAAAVARALA LPPHQRF LSSSSEELSSIYGSR HG E
Sbjct: 61  ARSLGLLPTTSDRPEVPARAVAAAAVARALAGLPPHQRFSLSSSSEELSSIYGSRNHGHE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELE+VFYEE+FDPVRH+LEHVPSEE+DLEY EKQATKRL QLDKVAERLSRH+MEHHE
Sbjct: 121 VEELEEVFYEEEFDPVRHVLEHVPSEENDLEYLEKQATKRLVQLDKVAERLSRHVMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALLLSSEYW 240
           VMVKGMHL RELEKDLKIANVICRNGKRHLNSSML VSRDLIVNSNSKKKQALL      
Sbjct: 181 VMVKGMHLVRELEKDLKIANVICRNGKRHLNSSMLEVSRDLIVNSNSKKKQALLDLLPVL 240

Query: 241 TNEHVRGSNDV-CLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE--- 300
           +   +R + D+  +L  L    N    +AFQVLSEYLQLLDSFSELSVI+EMS G+E   
Sbjct: 241 SE--LRHAVDMQSMLEILVEEGNY--YKAFQVLSEYLQLLDSFSELSVIQEMSRGVEIWL 300

Query: 301 -------------------------VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360
                                    VVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK
Sbjct: 301 GRTLQKLDSLLIEVCQEFKKEAYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHSALK 360

Query: 361 DIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLDTKD 420
           DIVQEDQDVH+QNSRLTYSDLCFRIPESKFRLCLLKTLAVLFT MCSYYQI SFQLDTKD
Sbjct: 361 DIVQEDQDVHVQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTSMCSYYQILSFQLDTKD 420

Query: 421 SIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNS---DGGDLNRGSPTDSSTVS 480
           SI++TP+MK QEDNYDI L D+ ESTI+VSS+G+  IT+S   D GD  R S TDSS  S
Sbjct: 421 SIERTPSMKHQEDNYDIKLGDSEESTIDVSSMGSTGITHSIYMDEGDSIRESRTDSSAAS 480

Query: 481 TSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540
           TSGSPWYHLRKDAI+FVSQTLQR RKNLWQL+TSRVSVLLSSAAVCSTSIHQFLKNYEDL
Sbjct: 481 TSGSPWYHLRKDAIDFVSQTLQRGRKNLWQLTTSRVSVLLSSAAVCSTSIHQFLKNYEDL 540

Query: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600
           NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV
Sbjct: 541 NVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDTVQV 600

Query: 601 VSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLLKQM 660
           VSF+GLVGDGAPLFV  +GNSS GK PRSDKSTSS+STGMDRSGFL+WL +GNPFLLK +
Sbjct: 601 VSFAGLVGDGAPLFVTSEGNSSNGKVPRSDKSTSSISTGMDRSGFLQWLNSGNPFLLKLI 660

Query: 661 HTCKEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADFIDE 720
           HT KEG PNG    E+DGS G    RSN+SP K+TD L+NGANTV EDE EDLLADFIDE
Sbjct: 661 HTYKEGTPNGTHYGEVDGSVGGSSHRSNASPTKFTDNLANGANTVSEDEDEDLLADFIDE 720

Query: 721 DSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG 780
           DSQLPSRI KP+LS NH SNHN D+ITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG
Sbjct: 721 DSQLPSRISKPKLSRNHCSNHNYDDITAQTGSSLCLLRSMDKYARLMQKLEIVNVEFFKG 780

Query: 781 MCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSSPSA 840
           MCQLF VFFYFVYETFGQL+T+SGGKGFPDSLNYKLKTALSRAAQDC+QW RPHSSSPSA
Sbjct: 781 MCQLFEVFFYFVYETFGQLSTSSGGKGFPDSLNYKLKTALSRAAQDCEQWIRPHSSSPSA 840

Query: 841 SNSTFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSMLLQT 891
           S+STF+FNEVTPSPPGSSLGY HGTS GLKER AGADSLSLVARIM+RSKAHIQSMLLQT
Sbjct: 841 SSSTFSFNEVTPSPPGSSLGYLHGTSFGLKERSAGADSLSLVARIMNRSKAHIQSMLLQT 900

BLAST of Cp4.1LG20g07520 vs. ExPASy TrEMBL
Match: A0A6J1DF95 (syndetin isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020518 PE=4 SV=1)

HSP 1 Score: 1396 bits (3614), Expect = 0.0
Identity = 762/939 (81.15%), Postives = 797/939 (84.88%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AFDGDLSEGF TSRFLFFVPFFLLQGGGMDLS+VGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFDGDLSEGFPTSRFLFFVPFFLLQGGGMDLSRVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLL  TSDRPEVPARAVAAAAVARALA LPPHQRF LSSSSEELSSIYGSR HGQE
Sbjct: 61  ARSLGLLPATSDRPEVPARAVAAAAVARALAGLPPHQRFSLSSSSEELSSIYGSRNHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELE+VFYEEDFDPVRH+LEHVPSEE+DLEY EKQATKRLAQLDKVAERLSRH+MEHHE
Sbjct: 121 VEELEEVFYEEDFDPVRHVLEHVPSEENDLEYLEKQATKRLAQLDKVAERLSRHVMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL----LS 240
           VMVKGMHL RELEKDLKIANVICRNGKRHL SSML VSRDLIVNSNSKKKQALL    + 
Sbjct: 181 VMVKGMHLVRELEKDLKIANVICRNGKRHLTSSMLEVSRDLIVNSNSKKKQALLDMLPVL 240

Query: 241 SEYWTNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE 300
           SE     HV     +  L  L    N    +AFQVLSEYLQLLDS SELSVI++MS G+E
Sbjct: 241 SEL---RHVVDMQSM--LESLVEEGNY--YKAFQVLSEYLQLLDSISELSVIQDMSRGVE 300

Query: 301 V----------------------------VDAYALIGDVSGLAEKIQSFFMQEVISETHS 360
           V                            VDAYALIGDVSGLAEKIQSFFMQEVISETHS
Sbjct: 301 VWLGRALQKLDSLLIEVCQEFKKEGYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHS 360

Query: 361 ALKDIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLD 420
           AL+DIVQEDQDVHIQNSRLTYSDLC  IPESKFR C LKTLAVLFTLMCSYYQI SFQLD
Sbjct: 361 ALRDIVQEDQDVHIQNSRLTYSDLCTWIPESKFRQCSLKTLAVLFTLMCSYYQILSFQLD 420

Query: 421 TKDSIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNS---DGGDLNRGSPTDSS 480
           TKDS DQTPNMKQQ+D+Y +NL DT E T NVSS+GT  I NS   D  D +R S  DSS
Sbjct: 421 TKDS-DQTPNMKQQDDSYVLNLGDTEELTTNVSSMGTTGIMNSVYMDDDDPDRESRMDSS 480

Query: 481 TVSTSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNY 540
           T STSGSPWYHLRKDAI+FVSQTLQR RKNLWQL+TSRVSVLLSSAAVCSTSIHQFLKNY
Sbjct: 481 TASTSGSPWYHLRKDAISFVSQTLQRGRKNLWQLTTSRVSVLLSSAAVCSTSIHQFLKNY 540

Query: 541 EDLNVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDT 600
           EDLNVFILAGEAFCGVEAVEFRQKLK VCENYYVAFHKQSMHALKMVMEKENWLTLPPDT
Sbjct: 541 EDLNVFILAGEAFCGVEAVEFRQKLKSVCENYYVAFHKQSMHALKMVMEKENWLTLPPDT 600

Query: 601 VQVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLL 660
           VQVVSF+GL+GDGAPLFV  DG+SS  K  R DKS+S V+TGMDRSGFL+WLK GNPFLL
Sbjct: 601 VQVVSFAGLIGDGAPLFVSSDGSSSNAKVSRKDKSSSLVNTGMDRSGFLQWLKGGNPFLL 660

Query: 661 KQMHTCKEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADF 720
           K MHT KEG PNG    ELDGS GE F R++ SP K TD L+NGANTV EDE EDLLADF
Sbjct: 661 KLMHTYKEGTPNGASYGELDGSVGESFCRNSVSPTKGTDNLANGANTVSEDEDEDLLADF 720

Query: 721 IDEDSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEF 780
           IDEDSQLPSR+ KP+L  NHSSNH NDE TAQTGSSLCLLRSMDKYARLMQKLEIVNVEF
Sbjct: 721 IDEDSQLPSRLSKPKLPRNHSSNHINDEFTAQTGSSLCLLRSMDKYARLMQKLEIVNVEF 780

Query: 781 FKGMCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSS 840
           FKG+CQLF VFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQW RPHSSS
Sbjct: 781 FKGICQLFGVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSS 840

Query: 841 PSASNSTFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSML 891
           PSASN+TF+F+EVTPSPPG+SLGY HGTS GLKER AGADSL LVARIM RSKAHIQSML
Sbjct: 841 PSASNTTFSFSEVTPSPPGTSLGYLHGTSFGLKERSAGADSLLLVARIMQRSKAHIQSML 900

BLAST of Cp4.1LG20g07520 vs. ExPASy TrEMBL
Match: A0A6J1DJ46 (syndetin isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020518 PE=4 SV=1)

HSP 1 Score: 1358 bits (3515), Expect = 0.0
Identity = 739/906 (81.57%), Postives = 774/906 (85.43%), Query Frame = 0

Query: 1   MQPNLFPFGSVLGNPSAFDGDLSEGFETSRFLFFVPFFLLQGGGMDLSKVGEKILSSVRS 60
           MQPNLFPFGSVLGNP AFDGDLSEGF TSRFLFFVPFFLLQGGGMDLS+VGEKILSSVRS
Sbjct: 1   MQPNLFPFGSVLGNPLAFDGDLSEGFPTSRFLFFVPFFLLQGGGMDLSRVGEKILSSVRS 60

Query: 61  ARSLGLLHTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELSSIYGSRIHGQE 120
           ARSLGLL  TSDRPEVPARAVAAAAVARALA LPPHQRF LSSSSEELSSIYGSR HGQE
Sbjct: 61  ARSLGLLPATSDRPEVPARAVAAAAVARALAGLPPHQRFSLSSSSEELSSIYGSRNHGQE 120

Query: 121 VEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAERLSRHLMEHHE 180
           VEELE+VFYEEDFDPVRH+LEHVPSEE+DLEY EKQATKRLAQLDKVAERLSRH+MEHHE
Sbjct: 121 VEELEEVFYEEDFDPVRHVLEHVPSEENDLEYLEKQATKRLAQLDKVAERLSRHVMEHHE 180

Query: 181 VMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKKKQALL----LS 240
           VMVKGMHL RELEKDLKIANVICRNGKRHL SSML VSRDLIVNSNSKKKQALL    + 
Sbjct: 181 VMVKGMHLVRELEKDLKIANVICRNGKRHLTSSMLEVSRDLIVNSNSKKKQALLDMLPVL 240

Query: 241 SEYWTNEHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVIREMSCGIE 300
           SE     HV     +  L  L    N    +AFQVLSEYLQLLDS SELSVI++MS G+E
Sbjct: 241 SEL---RHVVDMQSM--LESLVEEGNY--YKAFQVLSEYLQLLDSISELSVIQDMSRGVE 300

Query: 301 V----------------------------VDAYALIGDVSGLAEKIQSFFMQEVISETHS 360
           V                            VDAYALIGDVSGLAEKIQSFFMQEVISETHS
Sbjct: 301 VWLGRALQKLDSLLIEVCQEFKKEGYLTVVDAYALIGDVSGLAEKIQSFFMQEVISETHS 360

Query: 361 ALKDIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYYQIFSFQLD 420
           AL+DIVQEDQDVHIQNSRLTYSDLC  IPESKFR C LKTLAVLFTLMCSYYQI SFQLD
Sbjct: 361 ALRDIVQEDQDVHIQNSRLTYSDLCTWIPESKFRQCSLKTLAVLFTLMCSYYQILSFQLD 420

Query: 421 TKDSIDQTPNMKQQEDNYDINLRDTPESTINVSSVGTAAITNS---DGGDLNRGSPTDSS 480
           TKDS DQTPNMKQQ+D+Y +NL DT E T NVSS+GT  I NS   D  D +R S  DSS
Sbjct: 421 TKDS-DQTPNMKQQDDSYVLNLGDTEELTTNVSSMGTTGIMNSVYMDDDDPDRESRMDSS 480

Query: 481 TVSTSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVSVLLSSAAVCSTSIHQFLKNY 540
           T STSGSPWYHLRKDAI+FVSQTLQR RKNLWQL+TSRVSVLLSSAAVCSTSIHQFLKNY
Sbjct: 481 TASTSGSPWYHLRKDAISFVSQTLQRGRKNLWQLTTSRVSVLLSSAAVCSTSIHQFLKNY 540

Query: 541 EDLNVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQSMHALKMVMEKENWLTLPPDT 600
           EDLNVFILAGEAFCGVEAVEFRQKLK VCENYYVAFHKQSMHALKMVMEKENWLTLPPDT
Sbjct: 541 EDLNVFILAGEAFCGVEAVEFRQKLKSVCENYYVAFHKQSMHALKMVMEKENWLTLPPDT 600

Query: 601 VQVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVSTGMDRSGFLRWLKNGNPFLL 660
           VQVVSF+GL+GDGAPLFV  DG+SS  K  R DKS+S V+TGMDRSGFL+WLK GNPFLL
Sbjct: 601 VQVVSFAGLIGDGAPLFVSSDGSSSNAKVSRKDKSSSLVNTGMDRSGFLQWLKGGNPFLL 660

Query: 661 KQMHTCKEGIPNG----ELDGSAGEGFDRSNSSPMKYTDKLSNGANTVPEDESEDLLADF 720
           K MHT KEG PNG    ELDGS GE F R++ SP K TD L+NGANTV EDE EDLLADF
Sbjct: 661 KLMHTYKEGTPNGASYGELDGSVGESFCRNSVSPTKGTDNLANGANTVSEDEDEDLLADF 720

Query: 721 IDEDSQLPSRILKPRLSLNHSSNHNNDEITAQTGSSLCLLRSMDKYARLMQKLEIVNVEF 780
           IDEDSQLPSR+ KP+L  NHSSNH NDE TAQTGSSLCLLRSMDKYARLMQKLEIVNVEF
Sbjct: 721 IDEDSQLPSRLSKPKLPRNHSSNHINDEFTAQTGSSLCLLRSMDKYARLMQKLEIVNVEF 780

Query: 781 FKGMCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWRRPHSSS 840
           FKG+CQLF VFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQW RPHSSS
Sbjct: 781 FKGICQLFGVFFYFVYETFGQLNTTSGGKGFPDSLNYKLKTALSRAAQDCDQWIRPHSSS 840

Query: 841 PSASNSTFAFNEVTPSPPGSSLGYFHGTSLGLKERCAGADSLSLVARIMHRSKAHIQSML 867
           PSASN+TF+F+EVTPSPPG+SLGY HGTS GLKER AGADSL LVARIM RSKAHIQSML
Sbjct: 841 PSASNTTFSFSEVTPSPPGTSLGYLHGTSFGLKERSAGADSLLLVARIMQRSKAHIQSML 898

BLAST of Cp4.1LG20g07520 vs. TAIR 10
Match: AT2G27900.1 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2451, C-terminal (InterPro:IPR019514), Vacuolar protein sorting-associated protein 54 (InterPro:IPR019515); Has 316 Blast hits to 252 proteins in 92 species: Archae - 0; Bacteria - 2; Metazoa - 200; Fungi - 2; Plants - 68; Viruses - 0; Other Eukaryotes - 44 (source: NCBI BLink). )

HSP 1 Score: 932.9 bits (2410), Expect = 1.9e-271
Identity = 551/964 (57.16%), Postives = 667/964 (69.19%), Query Frame = 0

Query: 1   MQPNL-FPFGSVLGNPSAFD--GDLSE-----GFETSRFLFFVPFFLLQGGG-MDLSKVG 60
           MQPNL FPFGSVLGNP  F+  GDL+E      FE+SR  F +PF L QG G MDLSKVG
Sbjct: 1   MQPNLFFPFGSVLGNPFLFNGGGDLNELGSGAAFESSRVFFLLPFLLSQGTGVMDLSKVG 60

Query: 61  EKILSSVRSARSLGLL--HTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELS 120
           EK LSSV+SA SLGLL   + SDRPE+PARA AAAAVARALA LP  QR  +SS++ EL+
Sbjct: 61  EKFLSSVKSATSLGLLPSPSISDRPEIPARAAAAAAVARALAGLPSDQRLSISSTATELN 120

Query: 121 SIYGSRIHGQEVEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAE 180
           SIYG+R   Q+VEELE+ FYEEDFDPVRHILE+VP +ES+L YFEKQAT RL QLDKVAE
Sbjct: 121 SIYGNRPLPQQVEELEEGFYEEDFDPVRHILENVPDDESELAYFEKQATLRLVQLDKVAE 180

Query: 181 RLSRHLMEHHEVMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKK 240
            LS H+MEHHEVMVKGM+L RELEKDLKIANVIC+NG+R+L SSM   SRDLIV+++SKK
Sbjct: 181 TLSHHVMEHHEVMVKGMNLVRELEKDLKIANVICKNGRRNLTSSMNEASRDLIVHTHSKK 240

Query: 241 KQALLLSSEYWTN-EHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVI 300
           KQALL      T+  H R    V       ++ +    +AFQVLSEYLQLLDS SE S  
Sbjct: 241 KQALLDMLPILTDLRHAR----VMQSNLEDLVEDGNYCKAFQVLSEYLQLLDSLSEFSAA 300

Query: 301 REMSCGIE----------------------------VVDAYALIGDVSGLAEKIQSFFMQ 360
           +EM+ G+E                            V+DAYALIGDVSGLAEKIQSFFMQ
Sbjct: 301 QEMTRGVEVWLGRTLHKLDSLLLGVCQEFKEDSYVMVLDAYALIGDVSGLAEKIQSFFMQ 360

Query: 361 EVISETHSALKDIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYY 420
           EVISETHS LK IV ED     Q SRLTYSDLC + PESKFR CLL+TLAVLF L+ SY+
Sbjct: 361 EVISETHSVLKSIVGEDNSAATQYSRLTYSDLCLQTPESKFRQCLLRTLAVLFQLIYSYH 420

Query: 421 QIFSFQLDTKDSIDQTPNM--KQQEDNYDINLRDTPESTINVSSVGTAAIT--NSDGGDL 480
           +I SF  + +  I  +P++   Q  D+   +  D  +  +   S+    I+   SDG   
Sbjct: 421 EIMSFTPEKEAEILVSPSLATTQMVDSVTGSSCDPQDGGLLPGSIPPCTISAEESDGSGT 480

Query: 481 NRG-----------SPTDSSTVSTSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVS 540
           +             S      VS+S SPWY+LRK++  FVS+TLQR R+NLWQL+TSRVS
Sbjct: 481 SSSVQLASNIAIDESRNSEDRVSSSESPWYYLRKESAAFVSETLQRGRRNLWQLTTSRVS 540

Query: 541 VLLSSAAVCSTSIHQFLKNYEDLNVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQS 600
           VLLSS    STSIHQFLKNYEDL++FILAGEAFCG E V+FR+KLK VCENY+ AFH+QS
Sbjct: 541 VLLSSPGASSTSIHQFLKNYEDLSIFILAGEAFCGFEVVDFREKLKGVCENYFTAFHRQS 600

Query: 601 MHALKMVMEKENWLTLPPDTVQVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVS 660
           MHALKMV+EKE W  L PDTVQ ++F+GLVGDGAPL +     S   + P SDK ++S+ 
Sbjct: 601 MHALKMVLEKETWTKLSPDTVQAINFAGLVGDGAPLIISSRSASGSSRFPHSDKPSNSID 660

Query: 661 TGMDRSGFLRWLKNGNPFLLKQMHTCKEGIPNGELDGSAGEGFDRSNS---SPMKYTDKL 720
              +RSGF  WLK+GNPF  K  H  +E      ++G   EG D  +    +P     K 
Sbjct: 661 PSGNRSGFSYWLKSGNPFSAKLTH-YREDQDYSSINGEDHEGNDSIHDDVVNPKIRDIKR 720

Query: 721 SNGANTVPEDESEDLLADFIDEDSQLPSRILKPRLSLNHSSNHN-NDEITAQTGSSLCLL 780
            NG + V EDE+EDLLADFIDEDSQLP R      S   SS+ + ND++TAQTGSSLCLL
Sbjct: 721 INGGSPVSEDENEDLLADFIDEDSQLPRRSFTRSQSRTSSSHFSTNDDLTAQTGSSLCLL 780

Query: 781 RSMDKYARLMQKLEIVNVEFFKGMCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLK 840
           RSMDKYARLMQKLEIVN EFFKG+CQLF VFFYFV++ FGQ NT SGGKG  DS N++LK
Sbjct: 781 RSMDKYARLMQKLEIVNAEFFKGICQLFGVFFYFVFQVFGQENTNSGGKGVSDSFNHRLK 840

Query: 841 TALSRAAQDCDQWRRPH-SSSPSAS----NSTFAFNEVTPSPPGSSLGYFHGTSLGLKER 892
           + LSR +Q+C+QW +PH SSSPS+S    N+  +  +VTP+ P ++ G+  G S  LKER
Sbjct: 841 SCLSRISQECEQWIKPHVSSSPSSSLAFPNTVHSLADVTPASPLNTSGHLSGVSFSLKER 900

BLAST of Cp4.1LG20g07520 vs. TAIR 10
Match: AT2G27900.2 (CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2451, C-terminal (InterPro:IPR019514), Vacuolar protein sorting-associated protein 54 (InterPro:IPR019515); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 932.9 bits (2410), Expect = 1.9e-271
Identity = 551/964 (57.16%), Postives = 667/964 (69.19%), Query Frame = 0

Query: 1   MQPNL-FPFGSVLGNPSAFD--GDLSE-----GFETSRFLFFVPFFLLQGGG-MDLSKVG 60
           MQPNL FPFGSVLGNP  F+  GDL+E      FE+SR  F +PF L QG G MDLSKVG
Sbjct: 1   MQPNLFFPFGSVLGNPFLFNGGGDLNELGSGAAFESSRVFFLLPFLLSQGTGVMDLSKVG 60

Query: 61  EKILSSVRSARSLGLL--HTTSDRPEVPARAVAAAAVARALAVLPPHQRFGLSSSSEELS 120
           EK LSSV+SA SLGLL   + SDRPE+PARA AAAAVARALA LP  QR  +SS++ EL+
Sbjct: 61  EKFLSSVKSATSLGLLPSPSISDRPEIPARAAAAAAVARALAGLPSDQRLSISSTATELN 120

Query: 121 SIYGSRIHGQEVEELEDVFYEEDFDPVRHILEHVPSEESDLEYFEKQATKRLAQLDKVAE 180
           SIYG+R   Q+VEELE+ FYEEDFDPVRHILE+VP +ES+L YFEKQAT RL QLDKVAE
Sbjct: 121 SIYGNRPLPQQVEELEEGFYEEDFDPVRHILENVPDDESELAYFEKQATLRLVQLDKVAE 180

Query: 181 RLSRHLMEHHEVMVKGMHLFRELEKDLKIANVICRNGKRHLNSSMLAVSRDLIVNSNSKK 240
            LS H+MEHHEVMVKGM+L RELEKDLKIANVIC+NG+R+L SSM   SRDLIV+++SKK
Sbjct: 181 TLSHHVMEHHEVMVKGMNLVRELEKDLKIANVICKNGRRNLTSSMNEASRDLIVHTHSKK 240

Query: 241 KQALLLSSEYWTN-EHVRGSNDVCLLTFLAVLPNMPAIQAFQVLSEYLQLLDSFSELSVI 300
           KQALL      T+  H R    V       ++ +    +AFQVLSEYLQLLDS SE S  
Sbjct: 241 KQALLDMLPILTDLRHAR----VMQSNLEDLVEDGNYCKAFQVLSEYLQLLDSLSEFSAA 300

Query: 301 REMSCGIE----------------------------VVDAYALIGDVSGLAEKIQSFFMQ 360
           +EM+ G+E                            V+DAYALIGDVSGLAEKIQSFFMQ
Sbjct: 301 QEMTRGVEVWLGRTLHKLDSLLLGVCQEFKEDSYVMVLDAYALIGDVSGLAEKIQSFFMQ 360

Query: 361 EVISETHSALKDIVQEDQDVHIQNSRLTYSDLCFRIPESKFRLCLLKTLAVLFTLMCSYY 420
           EVISETHS LK IV ED     Q SRLTYSDLC + PESKFR CLL+TLAVLF L+ SY+
Sbjct: 361 EVISETHSVLKSIVGEDNSAATQYSRLTYSDLCLQTPESKFRQCLLRTLAVLFQLIYSYH 420

Query: 421 QIFSFQLDTKDSIDQTPNM--KQQEDNYDINLRDTPESTINVSSVGTAAIT--NSDGGDL 480
           +I SF  + +  I  +P++   Q  D+   +  D  +  +   S+    I+   SDG   
Sbjct: 421 EIMSFTPEKEAEILVSPSLATTQMVDSVTGSSCDPQDGGLLPGSIPPCTISAEESDGSGT 480

Query: 481 NRG-----------SPTDSSTVSTSGSPWYHLRKDAINFVSQTLQRSRKNLWQLSTSRVS 540
           +             S      VS+S SPWY+LRK++  FVS+TLQR R+NLWQL+TSRVS
Sbjct: 481 SSSVQLASNIAIDESRNSEDRVSSSESPWYYLRKESAAFVSETLQRGRRNLWQLTTSRVS 540

Query: 541 VLLSSAAVCSTSIHQFLKNYEDLNVFILAGEAFCGVEAVEFRQKLKIVCENYYVAFHKQS 600
           VLLSS    STSIHQFLKNYEDL++FILAGEAFCG E V+FR+KLK VCENY+ AFH+QS
Sbjct: 541 VLLSSPGASSTSIHQFLKNYEDLSIFILAGEAFCGFEVVDFREKLKGVCENYFTAFHRQS 600

Query: 601 MHALKMVMEKENWLTLPPDTVQVVSFSGLVGDGAPLFVHCDGNSSKGKAPRSDKSTSSVS 660
           MHALKMV+EKE W  L PDTVQ ++F+GLVGDGAPL +     S   + P SDK ++S+ 
Sbjct: 601 MHALKMVLEKETWTKLSPDTVQAINFAGLVGDGAPLIISSRSASGSSRFPHSDKPSNSID 660

Query: 661 TGMDRSGFLRWLKNGNPFLLKQMHTCKEGIPNGELDGSAGEGFDRSNS---SPMKYTDKL 720
              +RSGF  WLK+GNPF  K  H  +E      ++G   EG D  +    +P     K 
Sbjct: 661 PSGNRSGFSYWLKSGNPFSAKLTH-YREDQDYSSINGEDHEGNDSIHDDVVNPKIRDIKR 720

Query: 721 SNGANTVPEDESEDLLADFIDEDSQLPSRILKPRLSLNHSSNHN-NDEITAQTGSSLCLL 780
            NG + V EDE+EDLLADFIDEDSQLP R      S   SS+ + ND++TAQTGSSLCLL
Sbjct: 721 INGGSPVSEDENEDLLADFIDEDSQLPRRSFTRSQSRTSSSHFSTNDDLTAQTGSSLCLL 780

Query: 781 RSMDKYARLMQKLEIVNVEFFKGMCQLFAVFFYFVYETFGQLNTTSGGKGFPDSLNYKLK 840
           RSMDKYARLMQKLEIVN EFFKG+CQLF VFFYFV++ FGQ NT SGGKG  DS N++LK
Sbjct: 781 RSMDKYARLMQKLEIVNAEFFKGICQLFGVFFYFVFQVFGQENTNSGGKGVSDSFNHRLK 840

Query: 841 TALSRAAQDCDQWRRPH-SSSPSAS----NSTFAFNEVTPSPPGSSLGYFHGTSLGLKER 892
           + LSR +Q+C+QW +PH SSSPS+S    N+  +  +VTP+ P ++ G+  G S  LKER
Sbjct: 841 SCLSRISQECEQWIKPHVSSSPSSSLAFPNTVHSLADVTPASPLNTSGHLSGVSFSLKER 900