Cp4.1LG20g07470 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g07470 vs. ExPASy Swiss-Prot
Match: P93231 (Vacuolar protein sorting-associated protein 41 homolog OS=Solanum lycopersicum OX=4081 GN=VPS41 PE=2 SV=1)

HSP 1 Score: 1530.8 bits (3962), Expect = 0.0e+00
Identity = 754/961 (78.46%), Postives = 851/961 (88.55%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLA 60
           M+P  SENG++GDDER+EEEE+ EEEE  ++EE+EPRLKYQRMG SVPSLL++DAA+C+A
Sbjct: 1   MSPKPSENGIDGDDERDEEEEDSEEEEAEEEEEDEPRLKYQRMGASVPSLLSADAATCIA 60

Query: 61  VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINS 120
           VAERMIALGTH G VHILDFLGNQVKEF AHTA VNDL FDT+GEYVGSCSDDGSVVINS
Sbjct: 61  VAERMIALGTHGGAVHILDFLGNQVKEFAAHTAAVNDLCFDTDGEYVGSCSDDGSVVINS 120

Query: 121 LFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLHSGE 180
           LFTDERM+FEYHRP KAIALDPDYA+ +SRRF  GGLAG LYLN KKWLGY+DQVLHSGE
Sbjct: 121 LFTDERMKFEYHRPMKAIALDPDYARTSSRRFVTGGLAGQLYLNVKKWLGYRDQVLHSGE 180

Query: 181 GPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLV 240
           GPIHAVKWRTSL+AWAND GVKVYDA+NDQRITFIERPRG P PELLLP +VWQDD+ LV
Sbjct: 181 GPIHAVKWRTSLVAWANDTGVKVYDASNDQRITFIERPRGIPRPELLLPHIVWQDDSLLV 240

Query: 241 IGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLV 300
           IGWGTSVKIA IRT+Q+K ANGT   +H+ MSS+N+VD+VASFQT+Y I+GIAPFGD LV
Sbjct: 241 IGWGTSVKIALIRTTQSKGANGTY--KHMSMSSLNQVDIVASFQTSYFISGIAPFGDSLV 300

Query: 301 VLAYIP-EEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKDYS 360
           +LAYIP EE+GEK FS T PSRQGNAQRPE+RVVTWNNDEL+ D LPVHGFEHYKAKDYS
Sbjct: 301 ILAYIPGEEDGEKDFSSTIPSRQGNAQRPEVRVVTWNNDELATDALPVHGFEHYKAKDYS 360

Query: 361 LAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAV 420
           LAHAPF+GSSYAGGQWA+G EP+YYIVSPKD+VI KPRDAEDHI+WLL+HGWHEKALEAV
Sbjct: 361 LAHAPFSGSSYAGGQWAAGDEPLYYIVSPKDVVIAKPRDAEDHINWLLQHGWHEKALEAV 420

Query: 421 EAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPV 480
           EA QG+SELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGS SAWERWVFHFAHL QLPV
Sbjct: 421 EANQGQSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSASAWERWVFHFAHLRQLPV 480

Query: 481 LAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQFN 540
           L PYIPTENPRLRDTAYE+ALVALA+NP FHKDLL+T+KSWPP IYS  PV +AIEPQ N
Sbjct: 481 LVPYIPTENPRLRDTAYEVALVALATNPSFHKDLLSTVKSWPPRIYSTTPVFSAIEPQIN 540

Query: 541 TSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMML 600
           TSSMTD LKEALAELYVIDGQ++KAF LYADL+KPD+FDFIEK+NLH+A+REKV+QLMM+
Sbjct: 541 TSSMTDPLKEALAELYVIDGQHDKAFALYADLMKPDLFDFIEKHNLHDAVREKVLQLMMI 600

Query: 601 DCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQ 660
           DCKRAV L IQ ++LIPP+EVVSQL  A DKCD+RYF+HLYLHSLFEVN HAGKD+HD+Q
Sbjct: 601 DCKRAVLLLIQQRDLIPPSEVVSQLIAARDKCDYRYFLHLYLHSLFEVNLHAGKDYHDMQ 660

Query: 661 VELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDK 720
           VELYADYD KMLL FLRSSQHYTLEKAY+ICVKK+LL+EQVFILGRMGNAKQALAVII++
Sbjct: 661 VELYADYDPKMLLTFLRSSQHYTLEKAYEICVKKDLLKEQVFILGRMGNAKQALAVIINR 720

Query: 721 LGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEI 780
           LGDIEEA+EFVSMQ DDELWEELI+   HK EMVG+LLEHTVGNLDPLYIVNM+PN +EI
Sbjct: 721 LGDIEEAIEFVSMQQDDELWEELIQQSFHKPEMVGVLLEHTVGNLDPLYIVNMLPNDLEI 780

Query: 781 PRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGKR 840
           PRLRDRLVKI+TDYRTETSLRHGCNDILKAD VNLLVKYYKEA+ GV LS+E D+V  +R
Sbjct: 781 PRLRDRLVKIVTDYRTETSLRHGCNDILKADCVNLLVKYYKEAKRGVCLSDEVDDVSSRR 840

Query: 841 NENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCLIE 900
            E  VS   ++++S++ +EVKSKTRGG RCCICF+PFSI N+S+I FFCCHAYH TCL+E
Sbjct: 841 GEKSVSHLGERTMSLKSVEVKSKTRGGGRCCICFDPFSILNVSIIAFFCCHAYHTTCLME 900

Query: 901 STSNLDAKKGTGETQHDSVSDFDYDNG---EIEDDDEDE-----ADMGGPRMRCILCTTA 953
           S+ ++  KK  G     + S  +Y NG   + ED+DE+E     A  G   MRCILCTTA
Sbjct: 901 SSISVGGKKEAGVAAQRTTSYDEYPNGVNDDYEDEDEEEEEEEDATSGALPMRCILCTTA 959

BLAST of Cp4.1LG20g07470 vs. ExPASy Swiss-Prot
Match: P93043 (Vacuolar protein sorting-associated protein 41 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS41 PE=1 SV=3)

HSP 1 Score: 1434.1 bits (3711), Expect = 0.0e+00
Identity = 722/985 (73.30%), Postives = 833/985 (84.57%), Query Frame = 0

Query: 1   MAPILSENGVEGDDER------EEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASD 60
           MA +  ENGV+GDDER      EEEEEEEEEEE  D+ EEEPRLKYQRMGG+VP+LL++D
Sbjct: 1   MAAVPPENGVDGDDEREEEEEDEEEEEEEEEEENGDEAEEEPRLKYQRMGGNVPALLSND 60

Query: 61  AASCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDG 120
           AASC+AVA RMIALGTH GTV ILD LGNQVKEFRAHTA VND++FDTEGEY+GSCSDDG
Sbjct: 61  AASCIAVAARMIALGTHDGTVRILDLLGNQVKEFRAHTAPVNDINFDTEGEYIGSCSDDG 120

Query: 121 SVVINSLFT-DERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKD 180
           SVVINSLFT DE+M+F+YHRP KAI+LDPDY KK S+RF AGGLAGHLY+NSKKW G KD
Sbjct: 121 SVVINSLFTDDEKMKFDYHRPMKAISLDPDYTKKQSKRFVAGGLAGHLYMNSKKWFGNKD 180

Query: 181 QVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVW 240
           QVLHSGEGPIH+VKWR SLIAWAND GVKVYD A DQR+TFIE+PRGSP PE LLP LVW
Sbjct: 181 QVLHSGEGPIHSVKWRGSLIAWANDVGVKVYDTAKDQRVTFIEKPRGSPRPEALLPHLVW 240

Query: 241 QDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIA 300
           QDDT LVIGWGTSVKIASI++ Q +T       R + MSS+ +VD+VASFQT+Y I+GIA
Sbjct: 241 QDDTLLVIGWGTSVKIASIKSDQQQTG----TFRQIQMSSLTQVDIVASFQTSYYISGIA 300

Query: 301 PFGDVLVVLAYIP-EEEGEKSFSITAP-SRQGNAQRPEIRVVTWNNDELSNDTLPVHGFE 360
           PFGD LV+LAYIP E +GEK FS T   SRQGNAQRPEIR+V+WNNDEL+ D LPVHGFE
Sbjct: 301 PFGDSLVILAYIPIEGDGEKEFSSTTTLSRQGNAQRPEIRIVSWNNDELTMDALPVHGFE 360

Query: 361 HYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGW 420
           HYKAKDYSLAHAPF GSSYAGGQWA+G EP+YYIVSPKD+VI KPRDAEDHI+WLL+HG+
Sbjct: 361 HYKAKDYSLAHAPFPGSSYAGGQWAAGDEPLYYIVSPKDVVIAKPRDAEDHINWLLQHGF 420

Query: 421 HEKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHF 480
           HEKAL AVEA +G++EL+D+VG+ YLDHLIVERKYAEAASLCPKLLRGS SAWERWVFHF
Sbjct: 421 HEKALAAVEASEGRTELIDKVGAGYLDHLIVERKYAEAASLCPKLLRGSASAWERWVFHF 480

Query: 481 AHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVI 540
           A L QLPVL PY+PT+NPRL+DT YE+ALVALA+NP +HK+LL+ +KSWP  +YSAL VI
Sbjct: 481 AQLRQLPVLVPYMPTDNPRLKDTVYEVALVALATNPSYHKELLSAVKSWPRSVYSALTVI 540

Query: 541 AAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIRE 600
           +AIEPQ NTSSMTDALKEALAELYVIDGQY+KAF LYADLLKP++FDFIEKY+LHEAIR 
Sbjct: 541 SAIEPQLNTSSMTDALKEALAELYVIDGQYQKAFSLYADLLKPEVFDFIEKYSLHEAIRG 600

Query: 601 KVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQL---------FKAVDKCDFRYFMHLYLH 660
           KVVQLM+LDCKRA  LFIQ ++LIPP+EVV QL          KA  KCD RY+++LYLH
Sbjct: 601 KVVQLMLLDCKRATVLFIQNRDLIPPSEVVPQLLKAGKNPQVLKAGKKCDSRYYLYLYLH 660

Query: 661 SLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFI 720
           +LFEV+   GKDFHD+QVELYA+YD KMLLPFLRSSQHY LEKAY++CVKK+ LREQVF+
Sbjct: 661 ALFEVSHDTGKDFHDMQVELYAEYDTKMLLPFLRSSQHYKLEKAYELCVKKDFLREQVFV 720

Query: 721 LGRMGNAKQALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVG 780
           LGRMGNAKQALAVII+KLGDIEEAVEFVSMQHDD+LWEELIK CL+K EMVG+LLEHTVG
Sbjct: 721 LGRMGNAKQALAVIINKLGDIEEAVEFVSMQHDDDLWEELIKQCLNKPEMVGLLLEHTVG 780

Query: 781 NLDPLYIVNMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEA 840
           NLDPLYIVNMVPNG+EIPRLRDRLVKI+TDYRTETSLRHGCNDILK D VNLLVK + EA
Sbjct: 781 NLDPLYIVNMVPNGLEIPRLRDRLVKIVTDYRTETSLRHGCNDILKTDIVNLLVKCFNEA 840

Query: 841 RHGVYLSNEEDEVRGKRNENKVS-----QSIQKSLSVRMMEVKSKTRGGARCCICFNPFS 900
           R GV LS+E+D+ R KR +N  S       + KSLS++M EVKSKTRG  RCC+CF+P S
Sbjct: 841 RRGVCLSHEDDDSRAKREDNNRSSFSQRMVVDKSLSIKMTEVKSKTRGDTRCCMCFDPVS 900

Query: 901 IQNISVIVFFCCHAYHETCLIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEAD 956
           I+  +V+VFFCCHAYHETCL+++  + +  K    T+  S  ++ YDNG  E+++++E D
Sbjct: 901 IRGDTVVVFFCCHAYHETCLMDAAFSNNNHK---TTKGSSGYEYSYDNGVDEEEEDEEED 960

BLAST of Cp4.1LG20g07470 vs. ExPASy Swiss-Prot
Match: Q5KU39 (Vacuolar protein sorting-associated protein 41 homolog OS=Mus musculus OX=10090 GN=Vps41 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.0e-155
Identity = 316/904 (34.96%), Postives = 492/904 (54.42%), Query Frame = 0

Query: 14  DEREEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAG 73
           +E+E E  EE  +E  ++ EEEP+LKY+R+   V  +L  DAASC+ V ++ +ALGTH G
Sbjct: 5   EEQETESLEESTDESEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYG 64

Query: 74  TVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINSLFTDERMRFEYHR 133
            V++LD  GN  ++F      +N +S D  GE++G CS+DG + +  L++ E     +  
Sbjct: 65  KVYLLDVQGNITQKFDVSPVKINQISLDDSGEHMGVCSEDGKLQVFGLYSGEEFHETFDC 124

Query: 134 PTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG-YKDQVLHSGEGPIHAVKWRTSL 193
           P K IA+ P + + + ++F  GG    L L  + W+  +K  VLH GEG I +VKWR  L
Sbjct: 125 PIKIIAVHPQFVRSSCKQFVTGG--KKLLLFERTWMNRWKSSVLHEGEGNIRSVKWRGHL 184

Query: 194 IAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLVIGWGTSVKIASI 253
           IAWAN+ GVKV+D  + QRI+ + R   S  P++    L W+D+  L+IGWGTS+KI S+
Sbjct: 185 IAWANNMGVKVFDITSKQRISNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSIKICSV 244

Query: 254 RTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLVVLAYIPEEEGEK 313
           +             R +P      V++V+ F+T + I+G+AP  D LVVL+Y+ E     
Sbjct: 245 KERH------ASEMRDLP---SRYVEIVSQFETEFYISGLAPLCDQLVVLSYVKE----- 304

Query: 314 SFSITAPSRQGNAQRPEIRVV---TWNNDELSNDTLPVHGFEHYKAKDYSLAHAPFTGSS 373
              ++  + +    RP + ++       +E+S+D L V GF+  + +DY L         
Sbjct: 305 ---VSEKTEREYCARPRLDIIQPLPETCEEISSDALTVRGFQENECRDYHL--------E 364

Query: 374 YAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAVEAGQG--KSE 433
           Y+ G      E ++Y+VSP+D+V+ K RD +DHI WLLE   +E+AL A E  Q   K  
Sbjct: 365 YSEG------ESLFYVVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQRNIKRH 424

Query: 434 LLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPVLAPYIPTE 493
            + ++G  Y++HL+   +Y  AA  C K+L  + S WE  V+ F  + QL  ++PY+P  
Sbjct: 425 KILDIGLAYVNHLVERGEYDMAARKCQKILGKNASLWEYEVYKFKEIGQLKAISPYLPRG 484

Query: 494 NPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQFNTSSMTDAL 553
           +P L+   YEM L     +   ++   T I+ WP  +Y+   ++ A+       S    L
Sbjct: 485 DPVLKPLIYEMILHEFLESD--YEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTL 544

Query: 554 KEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRAVPL 613
            + LAELY  D  Y  A  +Y  L   D+F  I K+NL  +I++K+V LM  D ++AV +
Sbjct: 545 LKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDM 604

Query: 614 FIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYADYD 673
            +  ++ I   +VV +L       D     H+YLH LF+ + H G+ +H+ Q+ LYA+YD
Sbjct: 605 LLDNEDKISIKKVVEELE------DRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYD 664

Query: 674 IKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDKLGDIEEAV 733
              LLPFLR S H  LEKA +IC ++N + E V++L RMGN++ AL +I+++L D+++A+
Sbjct: 665 RPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAI 724

Query: 734 EFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEIPRLRDRLV 793
           EF   Q D ELWE+LI   + K   +  LL +   ++DP+ +++ +  G+EIP LRD LV
Sbjct: 725 EFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLV 784

Query: 794 KIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGKRNENKVSQS 853
           KI+ DY  +  LR GC  IL AD+++LL K ++    GV +  E                
Sbjct: 785 KILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEEN--------------- 832

Query: 854 IQKSLSVRMMEVKSKTRGGARCCICFNPF----SIQNISVIVFFCCHAYHETCLIESTSN 908
                                C  C +P     + +  SV+VF C H +H+ CL   + N
Sbjct: 845 --------------------ICESCLSPILPTDAAKPFSVVVFHCRHMFHKECLPMPSMN 832

BLAST of Cp4.1LG20g07470 vs. ExPASy Swiss-Prot
Match: P49754 (Vacuolar protein sorting-associated protein 41 homolog OS=Homo sapiens OX=9606 GN=VPS41 PE=1 SV=3)

HSP 1 Score: 548.5 bits (1412), Expect = 1.5e-154
Identity = 319/907 (35.17%), Postives = 492/907 (54.24%), Query Frame = 0

Query: 15  EREEEE----EEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGT 74
           E EE+E    EE  +E   ++ EEEP+LKY+R+   V  +L  DAASC+ V ++ +ALGT
Sbjct: 3   EAEEQETGSLEESTDESEEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGT 62

Query: 75  HAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINSLFTDERMRFE 134
           H G V++LD  GN  ++F      +N +S D  GE++G CS+DG V +  L++ E     
Sbjct: 63  HYGKVYLLDVQGNITQKFDVSPVKINQISLDESGEHMGVCSEDGKVQVFGLYSGEEFHET 122

Query: 135 YHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG-YKDQVLHSGEGPIHAVKWR 194
           +  P K IA+ P + + + ++F  GG    L L  + W+  +K  VLH GEG I +VKWR
Sbjct: 123 FDCPIKIIAVHPHFVRSSCKQFVTGG--KKLLLFERSWMNRWKSAVLHEGEGNIRSVKWR 182

Query: 195 TSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLVIGWGTSVKI 254
             LIAWAN+ GVK++D  + QRIT + R   S  P++    L W+D+  L+IGWGTSVK+
Sbjct: 183 GHLIAWANNMGVKIFDIISKQRITNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSVKV 242

Query: 255 ASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLVVLAYIPEEE 314
            S++             R +P      V++V+ F+T + I+G+AP  D LVVL+Y+ E  
Sbjct: 243 CSVKERH------ASEMRDLP---SRYVEIVSQFETEFYISGLAPLCDQLVVLSYVKE-- 302

Query: 315 GEKSFSITAPSRQGNAQRPEIRVV---TWNNDELSNDTLPVHGFEHYKAKDYSLAHAPFT 374
                 I+  + +    RP + ++   +   +E+S+D L V GF+  + +DY L      
Sbjct: 303 ------ISEKTEREYCARPRLDIIQPLSETCEEISSDALTVRGFQENECRDYHL------ 362

Query: 375 GSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAVEAGQG-- 434
              Y+ G      E ++YIVSP+D+V+ K RD +DHI WLLE   +E+AL A E  Q   
Sbjct: 363 --EYSEG------ESLFYIVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNI 422

Query: 435 KSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPVLAPYI 494
           K   + ++G  Y++HL+    Y  AA  C K+L  + + WE  V+ F  + QL  ++PY+
Sbjct: 423 KRHKILDIGLAYINHLVERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYL 482

Query: 495 PTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQFNTSSMT 554
           P  +P L+   YEM L     +   ++   T I+ WP  +Y+   ++ A+       S  
Sbjct: 483 PRGDPVLKPLIYEMILHEFLESD--YEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQN 542

Query: 555 DALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLDCKRA 614
             L + LAELY  D  Y  A  +Y  L   D+F  I K+NL  +I++K+V LM  D ++A
Sbjct: 543 KTLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKA 602

Query: 615 VPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYA 674
           V + +  ++ I   +VV +L       D     H+YLH LF+ + H G+ +H+ Q+ LYA
Sbjct: 603 VDMLLDNEDKISIKKVVEELE------DRPELQHVYLHKLFKRDHHKGQRYHEKQISLYA 662

Query: 675 DYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDKLGDIE 734
           +YD   LLPFLR S H  LEKA +IC ++N + E V++L RMGN++ AL +I+++L D++
Sbjct: 663 EYDRPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVD 722

Query: 735 EAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEIPRLRD 794
           +A+EF   Q D ELWE+LI   + K   +  LL +   ++DP+ +++ +  G+EIP LRD
Sbjct: 723 KAIEFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRD 782

Query: 795 RLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGKRNENKV 854
            LVKI+ DY  +  LR GC  IL AD+++LL K ++    GV +  E             
Sbjct: 783 SLVKILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEEN------------ 833

Query: 855 SQSIQKSLSVRMMEVKSKTRGGARCCICFNPF----SIQNISVIVFFCCHAYHETCLIES 908
                                   C  C +P     + +  SV+VF C H +H+ CL   
Sbjct: 843 -----------------------ICESCLSPILPSDAAKPFSVVVFHCRHMFHKECLPMP 833

BLAST of Cp4.1LG20g07470 vs. ExPASy Swiss-Prot
Match: Q9P7N3 (Vacuolar protein sorting-associated protein 41 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps41 PE=3 SV=2)

HSP 1 Score: 405.6 bits (1041), Expect = 1.6e-111
Identity = 249/822 (30.29%), Postives = 438/822 (53.28%), Query Frame = 0

Query: 17  EEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLAVAERMIALGTHAGTVH 76
           E   + E +   + DE+EEP+L Y+R+         +D  S  A+++     G+H G ++
Sbjct: 5   ESNSDSEIDSISSSDEDEEPKLIYERITEKFQGCFMNDTISACAISKEHFFFGSHNGAIY 64

Query: 77  ILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINSLFTDERMRFEYHRPTK 136
           I    G  +++   H+A V DLS D E E + SCS DG ++I+++ T E    ++ RP  
Sbjct: 65  IYQKNGILLRKMILHSASVVDLSVDLESENLASCSMDGKMIISNITTRETTVHDFKRPLL 124

Query: 137 AIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLHSGEGPIHAVKWRTSLIAWA 196
           ++A+DP Y+ ++SR+  +GG AG + L+ K WLG KD VL +  G ++ + W T+ IAWA
Sbjct: 125 SVAIDPYYSTRSSRQVLSGGRAGKVVLSEKGWLGNKDTVLQADCGAVYKISWYTTYIAWA 184

Query: 197 NDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLVIGWGTSVKIASIRTSQ 256
           +D G+ VY     + +  +E P+  P  E+   QL WQ +++LVIGW   + I SI+ S 
Sbjct: 185 SDLGITVYSTEFGKVLGRLEPPKRLPNDEIFPYQLFWQSESRLVIGWSDQIMIVSIQRS- 244

Query: 257 NKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLVVLAYIPEEEGEKSFSI 316
              AN            + ++ + A  + + +++G+   G  ++ LAYI   E    F+ 
Sbjct: 245 -NVAN-----------ELPKISLQALLEIDSIVSGVLMLGFNILTLAYIANVE---DFTS 304

Query: 317 TAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKDYSLAHAPFTGSSYAGGQWA 376
             PS++    RPE+R++  +  EL  D + +  +   +  DY L   P + S        
Sbjct: 305 AIPSQRIEGCRPELRLIDSSFKELCGDAIGLANYSRLQPSDYHLLPDPSSNSH------- 364

Query: 377 SGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAVE--AGQGKSELLDEVGS 436
                  +++SP DIV  + R+  DH+ +L+    + +A++AV+       S  + E+  
Sbjct: 365 ------SFVISPNDIVYVRERNQIDHVKYLVSKEMYAEAIDAVKKLPEIPPSLQISELAK 424

Query: 437 RYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPVLAPYIPTENPRLRDT 496
           +Y+ HL+ + +Y EA  + P L   +++ WE+WVF FA    L  +A ++PT    L   
Sbjct: 425 KYIFHLLGKGQYKEAGMVIPSLYNDNLAEWEQWVFVFAENDHLEDIADFLPTGENHLSPL 484

Query: 497 AYEMALVA-LASNP-LFHKDLLTTIKSWPPVIYSALPVIAAIEPQFNTSSMTDALKEALA 556
            YEM L   LA++   F+K L      WP ++YS   +  A   +F  +  +  L E+LA
Sbjct: 485 VYEMILAQYLATDERTFNKKL----HEWPTMLYSVSTIRNATLKKFKENQKSSTLTESLA 544

Query: 557 ELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMML-------DCKRAV 616
            LY+ D     AF LY  L      D I ++NL++  R  V+ LM++       D K A+
Sbjct: 545 FLYLEDNMPIDAFHLYLKLHSELCIDLILQHNLYDEARASVLLLMLISSKGKSSDTKSAM 604

Query: 617 -PLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQVELYA 676
             + +Q+    PP EV+ Q+  +V +  + YF    L     + P++  ++ D++++++A
Sbjct: 605 SSMLVQHVHSFPPQEVIMQI-HSVPQFLYEYFCEFEL-----MYPNSLMEYGDLKLDVFA 664

Query: 677 DYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDKLGDIE 736
           ++D K    FL ++Q Y+L+ A  IC + N L E V+ILGRMGN K+AL +II++L DI 
Sbjct: 665 EFDRKRFFDFLVNTQCYSLDHAAQICKQYNYLDELVYILGRMGNNKEALMLIINELLDIG 724

Query: 737 EAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEIPRLRD 796
            A+ +V  Q D ELW++LI   L K E +  LLE+   + +   +++ +P G ++P ++ 
Sbjct: 725 RAIRYVKEQADRELWDDLISYSLDKPEFICTLLENIGTDENARNLLSKIPPGTKLPHMKK 784

Query: 797 RLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGV 827
            + K++ D++++  L   C  + K +++++ +KY ++ + G+
Sbjct: 785 SISKLLADHQSQVQLYQSCYKLFKNESISMAIKYREQEQSGL 787

BLAST of Cp4.1LG20g07470 vs. NCBI nr
Match: XP_023519696.1 (vacuolar protein sorting-associated protein 41 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1910 bits (4948), Expect = 0.0
Identity = 955/955 (100.00%), Postives = 955/955 (100.00%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLA 60
           MAPILSENGVEGDDEREEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLA
Sbjct: 1   MAPILSENGVEGDDEREEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASCLA 60

Query: 61  VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINS 120
           VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINS
Sbjct: 61  VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVINS 120

Query: 121 LFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLHSGE 180
           LFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLHSGE
Sbjct: 121 LFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLHSGE 180

Query: 181 GPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLV 240
           GPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLV
Sbjct: 181 GPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQLV 240

Query: 241 IGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLV 300
           IGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLV
Sbjct: 241 IGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDVLV 300

Query: 301 VLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKDYSL 360
           VLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKDYSL
Sbjct: 301 VLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKDYSL 360

Query: 361 AHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAVE 420
           AHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAVE
Sbjct: 361 AHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEAVE 420

Query: 421 AGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPVL 480
           AGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPVL
Sbjct: 421 AGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLPVL 480

Query: 481 APYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQFNT 540
           APYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQFNT
Sbjct: 481 APYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQFNT 540

Query: 541 SSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLD 600
           SSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLD
Sbjct: 541 SSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMMLD 600

Query: 601 CKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQV 660
           CKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQV
Sbjct: 601 CKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDIQV 660

Query: 661 ELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDKL 720
           ELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDKL
Sbjct: 661 ELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIIDKL 720

Query: 721 GDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEIP 780
           GDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEIP
Sbjct: 721 GDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIEIP 780

Query: 781 RLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGKRN 840
           RLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGKRN
Sbjct: 781 RLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGKRN 840

Query: 841 ENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCLIES 900
           ENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCLIES
Sbjct: 841 ENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCLIES 900

Query: 901 TSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTASSKS 955
           TSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTASSKS
Sbjct: 901 TSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTASSKS 955

BLAST of Cp4.1LG20g07470 vs. NCBI nr
Match: XP_022964726.1 (vacuolar protein sorting-associated protein 41 homolog [Cucurbita moschata])

HSP 1 Score: 1883 bits (4879), Expect = 0.0
Identity = 947/965 (98.13%), Postives = 951/965 (98.55%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEEEEE-------MADDEEEE---PRLKYQRMGGSVPSL 60
           MAPILSENGVEGDDEREEEEEEEEEEE       MADDEEEE   PRLKYQRMGGSVPSL
Sbjct: 8   MAPILSENGVEGDDEREEEEEEEEEEEEEEEEEEMADDEEEEEEEPRLKYQRMGGSVPSL 67

Query: 61  LASDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSC 120
           LASDAASCL VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSC
Sbjct: 68  LASDAASCLVVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSC 127

Query: 121 SDDGSVVINSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG 180
           SDDGSVVINSLFTDERMRFEYHRP KAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG
Sbjct: 128 SDDGSVVINSLFTDERMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG 187

Query: 181 YKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQ 240
           YKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQ
Sbjct: 188 YKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQ 247

Query: 241 LVWQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLIT 300
           LVWQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLIT
Sbjct: 248 LVWQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLIT 307

Query: 301 GIAPFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGF 360
           GIAPFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGF
Sbjct: 308 GIAPFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGF 367

Query: 361 EHYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHG 420
           EHYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHG
Sbjct: 368 EHYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHG 427

Query: 421 WHEKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFH 480
           WHEKALEAVEAG GKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFH
Sbjct: 428 WHEKALEAVEAGHGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFH 487

Query: 481 FAHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPV 540
           FAHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPV
Sbjct: 488 FAHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPV 547

Query: 541 IAAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIR 600
           IAAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIR
Sbjct: 548 IAAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIR 607

Query: 601 EKVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPH 660
           EKVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPH
Sbjct: 608 EKVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPH 667

Query: 661 AGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAK 720
           AGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAK
Sbjct: 668 AGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAK 727

Query: 721 QALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIV 780
           QALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIV
Sbjct: 728 QALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIV 787

Query: 781 NMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSN 840
           NMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSN
Sbjct: 788 NMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSN 847

Query: 841 EEDEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCH 900
           E+DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCH
Sbjct: 848 EDDEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCH 907

Query: 901 AYHETCLIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTT 955
           AYHETCLIESTSNLDAKKGTGET+HDSVS+F+YDNGEIEDDDEDEADMGGPRMRCILCTT
Sbjct: 908 AYHETCLIESTSNLDAKKGTGETRHDSVSNFEYDNGEIEDDDEDEADMGGPRMRCILCTT 967

BLAST of Cp4.1LG20g07470 vs. NCBI nr
Match: KAG7019302.1 (Vacuolar protein sorting-associated protein 41-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1880 bits (4869), Expect = 0.0
Identity = 944/958 (98.54%), Postives = 950/958 (99.16%), Query Frame = 0

Query: 1   MAPILSENGVEGDDER--EEEEEEEEEEEMADDEEEE-PRLKYQRMGGSVPSLLASDAAS 60
           MAPILSENGVEGDDER  EEEEEEEEEEEMADDEEEE PRLKYQRMGGSVPSLLASDAAS
Sbjct: 1   MAPILSENGVEGDDERGEEEEEEEEEEEEMADDEEEEEPRLKYQRMGGSVPSLLASDAAS 60

Query: 61  CLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVV 120
           CL VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVV
Sbjct: 61  CLVVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVV 120

Query: 121 INSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLH 180
           INSLFTDERMRFEYHRP KAIALDPDYAKKTSRRFAAGGLAG+LYLNSKKWLGYKDQVLH
Sbjct: 121 INSLFTDERMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGNLYLNSKKWLGYKDQVLH 180

Query: 181 SGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDT 240
           SGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLVWQDDT
Sbjct: 181 SGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPCPELLLPQLVWQDDT 240

Query: 241 QLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGD 300
           QLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGD
Sbjct: 241 QLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGD 300

Query: 301 VLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKD 360
           VLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKD
Sbjct: 301 VLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKD 360

Query: 361 YSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALE 420
           YSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALE
Sbjct: 361 YSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALE 420

Query: 421 AVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQL 480
           AVEAG GKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQL
Sbjct: 421 AVEAGHGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQL 480

Query: 481 PVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQ 540
           PVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPV YSALPVIAAIEPQ
Sbjct: 481 PVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVNYSALPVIAAIEPQ 540

Query: 541 FNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLM 600
           FNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLM
Sbjct: 541 FNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLM 600

Query: 601 MLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHD 660
           MLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHD
Sbjct: 601 MLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHD 660

Query: 661 IQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVII 720
           IQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVII
Sbjct: 661 IQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVII 720

Query: 721 DKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGI 780
           DKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGI
Sbjct: 721 DKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGI 780

Query: 781 EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRG 840
           EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNE+DEVRG
Sbjct: 781 EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEDDEVRG 840

Query: 841 KRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCL 900
           KRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCL
Sbjct: 841 KRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCL 900

Query: 901 IESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTASSKS 955
           IESTSNLDAKKGTGET+HDSVS+F+YDNGEIE+DDEDEADMGGPRMRCILCTTASSKS
Sbjct: 901 IESTSNLDAKKGTGETRHDSVSNFEYDNGEIEEDDEDEADMGGPRMRCILCTTASSKS 958

BLAST of Cp4.1LG20g07470 vs. NCBI nr
Match: KAG6583600.1 (Vacuolar protein sorting-associated protein 41-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1880 bits (4869), Expect = 0.0
Identity = 944/958 (98.54%), Postives = 950/958 (99.16%), Query Frame = 0

Query: 1   MAPILSENGVEGDDER--EEEEEEEEEEEMADDEEEE-PRLKYQRMGGSVPSLLASDAAS 60
           MAPILSENGVEGDDER  EEEEEEEEEEEMADDEEEE PRLKYQRMGGSVPSLLASDAAS
Sbjct: 1   MAPILSENGVEGDDERGEEEEEEEEEEEEMADDEEEEEPRLKYQRMGGSVPSLLASDAAS 60

Query: 61  CLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVV 120
           CL VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVV
Sbjct: 61  CLVVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVV 120

Query: 121 INSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLH 180
           INSLFTDERMRFEYHRP KAIALDPDYAKKTSRRFAAGGLAG+LYLNSKKWLGYKDQVLH
Sbjct: 121 INSLFTDERMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGNLYLNSKKWLGYKDQVLH 180

Query: 181 SGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDT 240
           SGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLVWQDDT
Sbjct: 181 SGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPCPELLLPQLVWQDDT 240

Query: 241 QLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGD 300
           QLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGD
Sbjct: 241 QLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGD 300

Query: 301 VLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKD 360
           VLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKD
Sbjct: 301 VLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKD 360

Query: 361 YSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALE 420
           YSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALE
Sbjct: 361 YSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALE 420

Query: 421 AVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQL 480
           AVEAG GKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQL
Sbjct: 421 AVEAGHGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQL 480

Query: 481 PVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQ 540
           PVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQ
Sbjct: 481 PVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQ 540

Query: 541 FNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLM 600
           FNTSSMTDALKEALAELYVIDGQYEKAFLLYADL KPDIFDFIEKYNLHEAIREKVVQLM
Sbjct: 541 FNTSSMTDALKEALAELYVIDGQYEKAFLLYADLPKPDIFDFIEKYNLHEAIREKVVQLM 600

Query: 601 MLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHD 660
           MLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHD
Sbjct: 601 MLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHD 660

Query: 661 IQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVII 720
           IQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVII
Sbjct: 661 IQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVII 720

Query: 721 DKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGI 780
           DKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGI
Sbjct: 721 DKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGI 780

Query: 781 EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRG 840
           EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNE+DEVRG
Sbjct: 781 EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEDDEVRG 840

Query: 841 KRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCL 900
           KRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCL
Sbjct: 841 KRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCL 900

Query: 901 IESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTASSKS 955
           IESTSNLDAKKGTGET+HDSVS+F+YDNGEIE+DDEDEADMGGPRMRCILCTTASSKS
Sbjct: 901 IESTSNLDAKKGTGETRHDSVSNFEYDNGEIEEDDEDEADMGGPRMRCILCTTASSKS 958

BLAST of Cp4.1LG20g07470 vs. NCBI nr
Match: XP_022970290.1 (vacuolar protein sorting-associated protein 41 homolog [Cucurbita maxima])

HSP 1 Score: 1867 bits (4836), Expect = 0.0
Identity = 938/963 (97.40%), Postives = 944/963 (98.03%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEEEEE--------MADDEEEEPRLKYQRMGGSVPSLLA 60
           MAPILSENGVEGDDEREEEEEEEEEEE        MADDEEE P+LKYQRMGGSV SLLA
Sbjct: 1   MAPILSENGVEGDDEREEEEEEEEEEEEEEEEEEDMADDEEE-PKLKYQRMGGSVSSLLA 60

Query: 61  SDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSD 120
            DAASCLAVAERMIALGTHAGTVHILDFLGNQVKEF AHTAIVNDLSFDTEGEYVGSCSD
Sbjct: 61  RDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFSAHTAIVNDLSFDTEGEYVGSCSD 120

Query: 121 DGSVVINSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYK 180
           DGSVVINSLFTDERMRFEYHRP KAIALDPDY KKTSRRFAAGGLAGHLYLNSKKWLGYK
Sbjct: 121 DGSVVINSLFTDERMRFEYHRPMKAIALDPDYEKKTSRRFAAGGLAGHLYLNSKKWLGYK 180

Query: 181 DQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLV 240
           DQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLV
Sbjct: 181 DQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQLV 240

Query: 241 WQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGI 300
           WQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGI
Sbjct: 241 WQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGI 300

Query: 301 APFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEH 360
           APFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEH
Sbjct: 301 APFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEH 360

Query: 361 YKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWH 420
           YKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWH
Sbjct: 361 YKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWH 420

Query: 421 EKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFA 480
           EKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFA
Sbjct: 421 EKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFA 480

Query: 481 HLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIA 540
           HLCQLPVLAPYIPTENPRLRDT YEMALVALASNPLFHKDLLT IKSWP VIYSALPVIA
Sbjct: 481 HLCQLPVLAPYIPTENPRLRDTVYEMALVALASNPLFHKDLLTIIKSWPSVIYSALPVIA 540

Query: 541 AIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREK 600
           AIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHE IREK
Sbjct: 541 AIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEVIREK 600

Query: 601 VVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAG 660
           VVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAG
Sbjct: 601 VVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAG 660

Query: 661 KDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQA 720
           KDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAY+IC+KKNLLREQVFILGRMGNAKQA
Sbjct: 661 KDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYEICIKKNLLREQVFILGRMGNAKQA 720

Query: 721 LAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNM 780
           LAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNM
Sbjct: 721 LAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNM 780

Query: 781 VPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEE 840
           VPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNE+
Sbjct: 781 VPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNED 840

Query: 841 DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAY 900
           DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAY
Sbjct: 841 DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAY 900

Query: 901 HETCLIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTAS 955
           HETCLIESTSNLDAKKGTGET+HDSVSDF+YDNGEIEDDDEDEADMGGPRMRCILCTTAS
Sbjct: 901 HETCLIESTSNLDAKKGTGETRHDSVSDFNYDNGEIEDDDEDEADMGGPRMRCILCTTAS 960

BLAST of Cp4.1LG20g07470 vs. ExPASy TrEMBL
Match: A0A6J1HJR5 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucurbita moschata OX=3662 GN=LOC111464717 PE=3 SV=1)

HSP 1 Score: 1883 bits (4879), Expect = 0.0
Identity = 947/965 (98.13%), Postives = 951/965 (98.55%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEEEEE-------MADDEEEE---PRLKYQRMGGSVPSL 60
           MAPILSENGVEGDDEREEEEEEEEEEE       MADDEEEE   PRLKYQRMGGSVPSL
Sbjct: 8   MAPILSENGVEGDDEREEEEEEEEEEEEEEEEEEMADDEEEEEEEPRLKYQRMGGSVPSL 67

Query: 61  LASDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSC 120
           LASDAASCL VAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSC
Sbjct: 68  LASDAASCLVVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSC 127

Query: 121 SDDGSVVINSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG 180
           SDDGSVVINSLFTDERMRFEYHRP KAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG
Sbjct: 128 SDDGSVVINSLFTDERMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG 187

Query: 181 YKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQ 240
           YKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQ
Sbjct: 188 YKDQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQ 247

Query: 241 LVWQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLIT 300
           LVWQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLIT
Sbjct: 248 LVWQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLIT 307

Query: 301 GIAPFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGF 360
           GIAPFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGF
Sbjct: 308 GIAPFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGF 367

Query: 361 EHYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHG 420
           EHYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHG
Sbjct: 368 EHYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHG 427

Query: 421 WHEKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFH 480
           WHEKALEAVEAG GKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFH
Sbjct: 428 WHEKALEAVEAGHGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFH 487

Query: 481 FAHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPV 540
           FAHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPV
Sbjct: 488 FAHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPV 547

Query: 541 IAAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIR 600
           IAAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIR
Sbjct: 548 IAAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIR 607

Query: 601 EKVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPH 660
           EKVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPH
Sbjct: 608 EKVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPH 667

Query: 661 AGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAK 720
           AGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAK
Sbjct: 668 AGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAK 727

Query: 721 QALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIV 780
           QALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIV
Sbjct: 728 QALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIV 787

Query: 781 NMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSN 840
           NMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSN
Sbjct: 788 NMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSN 847

Query: 841 EEDEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCH 900
           E+DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCH
Sbjct: 848 EDDEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCH 907

Query: 901 AYHETCLIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTT 955
           AYHETCLIESTSNLDAKKGTGET+HDSVS+F+YDNGEIEDDDEDEADMGGPRMRCILCTT
Sbjct: 908 AYHETCLIESTSNLDAKKGTGETRHDSVSNFEYDNGEIEDDDEDEADMGGPRMRCILCTT 967

BLAST of Cp4.1LG20g07470 vs. ExPASy TrEMBL
Match: A0A6J1I2G3 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469300 PE=3 SV=1)

HSP 1 Score: 1867 bits (4836), Expect = 0.0
Identity = 938/963 (97.40%), Postives = 944/963 (98.03%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEEEEE--------MADDEEEEPRLKYQRMGGSVPSLLA 60
           MAPILSENGVEGDDEREEEEEEEEEEE        MADDEEE P+LKYQRMGGSV SLLA
Sbjct: 1   MAPILSENGVEGDDEREEEEEEEEEEEEEEEEEEDMADDEEE-PKLKYQRMGGSVSSLLA 60

Query: 61  SDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSD 120
            DAASCLAVAERMIALGTHAGTVHILDFLGNQVKEF AHTAIVNDLSFDTEGEYVGSCSD
Sbjct: 61  RDAASCLAVAERMIALGTHAGTVHILDFLGNQVKEFSAHTAIVNDLSFDTEGEYVGSCSD 120

Query: 121 DGSVVINSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYK 180
           DGSVVINSLFTDERMRFEYHRP KAIALDPDY KKTSRRFAAGGLAGHLYLNSKKWLGYK
Sbjct: 121 DGSVVINSLFTDERMRFEYHRPMKAIALDPDYEKKTSRRFAAGGLAGHLYLNSKKWLGYK 180

Query: 181 DQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLV 240
           DQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLV
Sbjct: 181 DQVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQLV 240

Query: 241 WQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGI 300
           WQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGI
Sbjct: 241 WQDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGI 300

Query: 301 APFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEH 360
           APFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEH
Sbjct: 301 APFGDVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEH 360

Query: 361 YKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWH 420
           YKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWH
Sbjct: 361 YKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWH 420

Query: 421 EKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFA 480
           EKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFA
Sbjct: 421 EKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFA 480

Query: 481 HLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIA 540
           HLCQLPVLAPYIPTENPRLRDT YEMALVALASNPLFHKDLLT IKSWP VIYSALPVIA
Sbjct: 481 HLCQLPVLAPYIPTENPRLRDTVYEMALVALASNPLFHKDLLTIIKSWPSVIYSALPVIA 540

Query: 541 AIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREK 600
           AIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHE IREK
Sbjct: 541 AIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEVIREK 600

Query: 601 VVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAG 660
           VVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAG
Sbjct: 601 VVQLMMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAG 660

Query: 661 KDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQA 720
           KDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAY+IC+KKNLLREQVFILGRMGNAKQA
Sbjct: 661 KDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYEICIKKNLLREQVFILGRMGNAKQA 720

Query: 721 LAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNM 780
           LAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNM
Sbjct: 721 LAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNM 780

Query: 781 VPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEE 840
           VPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNE+
Sbjct: 781 VPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNED 840

Query: 841 DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAY 900
           DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAY
Sbjct: 841 DEVRGKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAY 900

Query: 901 HETCLIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTAS 955
           HETCLIESTSNLDAKKGTGET+HDSVSDF+YDNGEIEDDDEDEADMGGPRMRCILCTTAS
Sbjct: 901 HETCLIESTSNLDAKKGTGETRHDSVSDFNYDNGEIEDDDEDEADMGGPRMRCILCTTAS 960

BLAST of Cp4.1LG20g07470 vs. ExPASy TrEMBL
Match: A0A1S3CGC3 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucumis melo OX=3656 GN=LOC103500583 PE=3 SV=1)

HSP 1 Score: 1791 bits (4640), Expect = 0.0
Identity = 892/961 (92.82%), Postives = 924/961 (96.15%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEEEE----EEEMADDEEEEPRLKYQRMGGSVPSLLASDAA 60
           MAPILS NGVEGDDEREEEEE++E    EEEMADDEEE PRLKYQRMGGSVPSLLASDAA
Sbjct: 1   MAPILSVNGVEGDDEREEEEEDDEDDDEEEEMADDEEE-PRLKYQRMGGSVPSLLASDAA 60

Query: 61  SCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSV 120
           SCLAVAERMIALGTHAGTVHILDFLGNQVKEF AHTA+VNDLSFDTEGEYVGSCSDDGSV
Sbjct: 61  SCLAVAERMIALGTHAGTVHILDFLGNQVKEFPAHTAVVNDLSFDTEGEYVGSCSDDGSV 120

Query: 121 VINSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVL 180
           VINSLFTDERMRFEYHRP KAIALDPDYAKKTSRRFAAGGLAGHLY NSKKWLGYKDQVL
Sbjct: 121 VINSLFTDERMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGHLYFNSKKWLGYKDQVL 180

Query: 181 HSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDD 240
           HSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLVWQDD
Sbjct: 181 HSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQLVWQDD 240

Query: 241 TQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFG 300
           T LVIGWGTSVKIASIRT+ N+ ANGTQ+SRHVP SSMNRVD+VASFQT+YLITG+APFG
Sbjct: 241 TLLVIGWGTSVKIASIRTNHNRAANGTQSSRHVPTSSMNRVDIVASFQTSYLITGMAPFG 300

Query: 301 DVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAK 360
           DVLVVLAYIP EEGEK FSITAPSRQGNAQRPE+RVVTWNNDELS D  PVHGFEHYKAK
Sbjct: 301 DVLVVLAYIPGEEGEKDFSITAPSRQGNAQRPEVRVVTWNNDELSTDARPVHGFEHYKAK 360

Query: 361 DYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKAL 420
           DYSLAHAPF GSSYAGGQWA+GVEP+YYIVSPKDIVI KPRDAEDHI+WLLEHGWHEKAL
Sbjct: 361 DYSLAHAPFAGSSYAGGQWAAGVEPLYYIVSPKDIVIAKPRDAEDHIAWLLEHGWHEKAL 420

Query: 421 EAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQ 480
           EAVEAGQG+SELLDEVGS+YLDHLIVERKYAEAASLCPKLLRGS SAWERWVFHFAHL Q
Sbjct: 421 EAVEAGQGRSELLDEVGSKYLDHLIVERKYAEAASLCPKLLRGSASAWERWVFHFAHLRQ 480

Query: 481 LPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEP 540
           LPVL PYIPTENPRLRDTAYE+ALVALASN LFHKDLLTT+K+WPPVIYSA+PVI+AIEP
Sbjct: 481 LPVLVPYIPTENPRLRDTAYEVALVALASNSLFHKDLLTTVKTWPPVIYSAVPVISAIEP 540

Query: 541 QFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQL 600
           QFNTSSMTDALKEALAELYV+DGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQL
Sbjct: 541 QFNTSSMTDALKEALAELYVVDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQL 600

Query: 601 MMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFH 660
           MMLDCKRAV LFIQ KELIPPNEVVSQLFKA DKCDFRYF+HLYLHSLFEVNPHAGKDFH
Sbjct: 601 MMLDCKRAVQLFIQNKELIPPNEVVSQLFKAGDKCDFRYFLHLYLHSLFEVNPHAGKDFH 660

Query: 661 DIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVI 720
           DIQVELYADYD KMLLPFLRSSQHYTLEKAYDIC+KKNLLREQVFILGRMGNAKQALAVI
Sbjct: 661 DIQVELYADYDTKMLLPFLRSSQHYTLEKAYDICIKKNLLREQVFILGRMGNAKQALAVI 720

Query: 721 IDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG 780
           IDKLGDIEEAVEFVSMQHDD+LWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG
Sbjct: 721 IDKLGDIEEAVEFVSMQHDDDLWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG 780

Query: 781 IEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVR 840
           +EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHG+YLSNEEDE R
Sbjct: 781 LEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGIYLSNEEDEAR 840

Query: 841 GKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETC 900
           GKRNENKVSQSIQKSL+VRMMEVKSKTRGG RCCICFNPFSIQNISVIVFFCCHAYHETC
Sbjct: 841 GKRNENKVSQSIQKSLNVRMMEVKSKTRGGTRCCICFNPFSIQNISVIVFFCCHAYHETC 900

Query: 901 LIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDD--EDEADMGGPRMRCILCTTASSK 955
           LIESTSNLDAKKGTGET HD  SDFDYDNGE+EDD+  ED+ DMGGPRMRCILCTTA+SK
Sbjct: 901 LIESTSNLDAKKGTGETGHDLASDFDYDNGEMEDDEDGEDDTDMGGPRMRCILCTTAASK 960

BLAST of Cp4.1LG20g07470 vs. ExPASy TrEMBL
Match: A0A0A0LY23 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucumis sativus OX=3659 GN=Csa_1G144290 PE=3 SV=1)

HSP 1 Score: 1788 bits (4630), Expect = 0.0
Identity = 891/961 (92.72%), Postives = 923/961 (96.05%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEE----EEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAA 60
           MAPILSENGVEGDDEREEEEE+    EEEEE+ADDEEE PRLKYQRMGGSVPSLLASDAA
Sbjct: 1   MAPILSENGVEGDDEREEEEEDDEDDEEEEEIADDEEE-PRLKYQRMGGSVPSLLASDAA 60

Query: 61  SCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSV 120
           SCLAVAERMIALGTHAGTVHILDFLGNQVKEF AHTA+VNDLSFDTEGEYVGSCSDDGSV
Sbjct: 61  SCLAVAERMIALGTHAGTVHILDFLGNQVKEFPAHTAVVNDLSFDTEGEYVGSCSDDGSV 120

Query: 121 VINSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVL 180
           VINSLFTDERMRFEYHRP KAIALDPDYAKKTSRRFAAGGLAGHLY NSKKWLG+KDQVL
Sbjct: 121 VINSLFTDERMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGHLYFNSKKWLGFKDQVL 180

Query: 181 HSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDD 240
           HSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLVWQDD
Sbjct: 181 HSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQLVWQDD 240

Query: 241 TQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFG 300
           T LVIGWGTSVKIASIRT+ N+ ANGTQ+SRHVP SSMNRVD+VASFQT+YLITG+APFG
Sbjct: 241 TLLVIGWGTSVKIASIRTNHNRAANGTQSSRHVPTSSMNRVDIVASFQTSYLITGMAPFG 300

Query: 301 DVLVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAK 360
           DVLVVLAYIP EEGEK FS+TAPSRQGNAQRPE+RVVTWNNDELS D LPVHGFEHYKAK
Sbjct: 301 DVLVVLAYIPGEEGEKDFSMTAPSRQGNAQRPEVRVVTWNNDELSTDALPVHGFEHYKAK 360

Query: 361 DYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKAL 420
           DYSLAHAPF GSSYAGGQWA+GVEP+YYIVSPKDIVI KPRDAEDHI+WLLEHGWHEKAL
Sbjct: 361 DYSLAHAPFAGSSYAGGQWAAGVEPLYYIVSPKDIVIAKPRDAEDHIAWLLEHGWHEKAL 420

Query: 421 EAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQ 480
           EAVEAGQG+SELLDEVGS+YLDHLIVERKYAEAA LCPKLLRGS SAWERWVFHFAHL Q
Sbjct: 421 EAVEAGQGRSELLDEVGSKYLDHLIVERKYAEAALLCPKLLRGSASAWERWVFHFAHLRQ 480

Query: 481 LPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEP 540
           LPVL PYIPTENPRLRDTAYE+ALVALASN  FHKDLLTT+K+WPPVIYSA+PVI+AIEP
Sbjct: 481 LPVLVPYIPTENPRLRDTAYEVALVALASNSSFHKDLLTTVKTWPPVIYSAVPVISAIEP 540

Query: 541 QFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQL 600
           QFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQL
Sbjct: 541 QFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQL 600

Query: 601 MMLDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFH 660
           MMLDCKRAV LFIQ KELIPPNEVVSQLFKA DKCDFRYF+HLYLHSLFEVNPHAGKDFH
Sbjct: 601 MMLDCKRAVQLFIQNKELIPPNEVVSQLFKAGDKCDFRYFLHLYLHSLFEVNPHAGKDFH 660

Query: 661 DIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVI 720
           DIQVELYADYD KMLLPFLRSSQHYTLEKAYDIC+KKNLLREQVFILGRMGNAKQALAVI
Sbjct: 661 DIQVELYADYDTKMLLPFLRSSQHYTLEKAYDICIKKNLLREQVFILGRMGNAKQALAVI 720

Query: 721 IDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG 780
           IDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG
Sbjct: 721 IDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG 780

Query: 781 IEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVR 840
           +EIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHG+YLSNEEDE R
Sbjct: 781 LEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGIYLSNEEDEAR 840

Query: 841 GKRNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETC 900
           GKRNENKVSQSIQKSL+VRMMEVKSKTRGG RCCICFNPFSIQNISVIVFFCCHAYHETC
Sbjct: 841 GKRNENKVSQSIQKSLNVRMMEVKSKTRGGTRCCICFNPFSIQNISVIVFFCCHAYHETC 900

Query: 901 LIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDD--EDEADMGGPRMRCILCTTASSK 955
           LIESTSNLDA KGTGET HD  SDFDYDNGEIEDD+  ED+ D+GGPRMRCILCTTA+SK
Sbjct: 901 LIESTSNLDAMKGTGETGHDLTSDFDYDNGEIEDDEDGEDDTDVGGPRMRCILCTTAASK 960

BLAST of Cp4.1LG20g07470 vs. ExPASy TrEMBL
Match: A0A6J1FDJ3 (Vacuolar protein sorting-associated protein 41 homolog OS=Cucurbita moschata OX=3662 GN=LOC111444390 PE=3 SV=1)

HSP 1 Score: 1783 bits (4617), Expect = 0.0
Identity = 884/957 (92.37%), Postives = 924/957 (96.55%), Query Frame = 0

Query: 1   MAPILSENGVEGDDEREEEEEE--EEEEEMADDEEEEPRLKYQRMGGSVPSLLASDAASC 60
           MAPILSEN VEGDDEREEEEEE  EEEEEMADDE E PRLKYQRMGGSVPSLLASDAASC
Sbjct: 1   MAPILSENSVEGDDEREEEEEEDEEEEEEMADDEME-PRLKYQRMGGSVPSLLASDAASC 60

Query: 61  LAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDGSVVI 120
           LAVAERMIALGTHAGT+HILDFLGNQVKEF AHTA+VNDLSFD+EGEYVGSCSDDGSVVI
Sbjct: 61  LAVAERMIALGTHAGTIHILDFLGNQVKEFPAHTAVVNDLSFDSEGEYVGSCSDDGSVVI 120

Query: 121 NSLFTDERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKDQVLHS 180
           NSLFTDE+MRFEYHRP KAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLG+KDQVLHS
Sbjct: 121 NSLFTDEKMRFEYHRPMKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGFKDQVLHS 180

Query: 181 GEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVWQDDTQ 240
           GEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSP PELLLPQLVWQDDT 
Sbjct: 181 GEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPRPELLLPQLVWQDDTL 240

Query: 241 LVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIAPFGDV 300
           LVIGWGTSVKIASIRT+QN+ ANGTQ+SR VPMSSMNRVD+VASFQT+YLITG+APFGD 
Sbjct: 241 LVIGWGTSVKIASIRTNQNRAANGTQSSRLVPMSSMNRVDIVASFQTSYLITGMAPFGDF 300

Query: 301 LVVLAYIPEEEGEKSFSITAPSRQGNAQRPEIRVVTWNNDELSNDTLPVHGFEHYKAKDY 360
           LVVLAYIP EEGEK FS TAPSRQGNAQRPE+R+VTWNNDELS D LPVHGFEHYKAKDY
Sbjct: 301 LVVLAYIPGEEGEKDFSRTAPSRQGNAQRPEVRIVTWNNDELSTDALPVHGFEHYKAKDY 360

Query: 361 SLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGWHEKALEA 420
           SLAHAPF GSSYAGGQWA+G EP+YY+VSPKD+VI KPRDAEDHI+WLLEHG+HEKALEA
Sbjct: 361 SLAHAPFAGSSYAGGQWAAGDEPLYYVVSPKDVVIAKPRDAEDHIAWLLEHGFHEKALEA 420

Query: 421 VEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLCQLP 480
           VEAGQG+SELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHL QLP
Sbjct: 421 VEAGQGRSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHFAHLRQLP 480

Query: 481 VLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVIAAIEPQF 540
           VL PYIPTENPRLRDTAYE+ALVALASNPLFHKDLLTT+KSWPPVIYSALPVI+AIEPQF
Sbjct: 481 VLVPYIPTENPRLRDTAYEVALVALASNPLFHKDLLTTVKSWPPVIYSALPVISAIEPQF 540

Query: 541 NTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMM 600
           NTSSMTDALKEALAELYVIDGQYEK FLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMM
Sbjct: 541 NTSSMTDALKEALAELYVIDGQYEKGFLLYADLLKPDIFDFIEKYNLHEAIREKVVQLMM 600

Query: 601 LDCKRAVPLFIQYKELIPPNEVVSQLFKAVDKCDFRYFMHLYLHSLFEVNPHAGKDFHDI 660
           LDCKRAVPLFIQ KELIPPNEVVSQLFKA DKCDFRYFMHLYLHSLFEVNPHAGKDFHDI
Sbjct: 601 LDCKRAVPLFIQNKELIPPNEVVSQLFKAGDKCDFRYFMHLYLHSLFEVNPHAGKDFHDI 660

Query: 661 QVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFILGRMGNAKQALAVIID 720
           QVELYADYDIKMLLPFLRSSQHYTLEKA++IC++KNLLREQVFILGRMGNAKQAL+VIID
Sbjct: 661 QVELYADYDIKMLLPFLRSSQHYTLEKAHEICIQKNLLREQVFILGRMGNAKQALSVIID 720

Query: 721 KLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGIE 780
           KLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNG+E
Sbjct: 721 KLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVGNLDPLYIVNMVPNGLE 780

Query: 781 IPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEARHGVYLSNEEDEVRGK 840
           IPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYY EARHG+YLSNEEDE+RGK
Sbjct: 781 IPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYNEARHGIYLSNEEDEIRGK 840

Query: 841 RNENKVSQSIQKSLSVRMMEVKSKTRGGARCCICFNPFSIQNISVIVFFCCHAYHETCLI 900
           R+ENKV QSIQ+S+S RMMEVKSKTRGGARCCICF+PFSIQN+SVIVFFCCHAYHETCLI
Sbjct: 841 RSENKVFQSIQESVSARMMEVKSKTRGGARCCICFDPFSIQNMSVIVFFCCHAYHETCLI 900

Query: 901 ESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEADMGGPRMRCILCTTASSKS 955
           ESTSNLDA KG+GET+HDS SDFDYDNGE EDDDED+ D GGPRMRCILCTTA+SKS
Sbjct: 901 ESTSNLDANKGSGETRHDSTSDFDYDNGEFEDDDEDDTDAGGPRMRCILCTTAASKS 956

BLAST of Cp4.1LG20g07470 vs. TAIR 10
Match: AT1G08190.1 (vacuolar protein sorting 41 )

HSP 1 Score: 1434.1 bits (3711), Expect = 0.0e+00
Identity = 722/985 (73.30%), Postives = 833/985 (84.57%), Query Frame = 0

Query: 1   MAPILSENGVEGDDER------EEEEEEEEEEEMADDEEEEPRLKYQRMGGSVPSLLASD 60
           MA +  ENGV+GDDER      EEEEEEEEEEE  D+ EEEPRLKYQRMGG+VP+LL++D
Sbjct: 1   MAAVPPENGVDGDDEREEEEEDEEEEEEEEEEENGDEAEEEPRLKYQRMGGNVPALLSND 60

Query: 61  AASCLAVAERMIALGTHAGTVHILDFLGNQVKEFRAHTAIVNDLSFDTEGEYVGSCSDDG 120
           AASC+AVA RMIALGTH GTV ILD LGNQVKEFRAHTA VND++FDTEGEY+GSCSDDG
Sbjct: 61  AASCIAVAARMIALGTHDGTVRILDLLGNQVKEFRAHTAPVNDINFDTEGEYIGSCSDDG 120

Query: 121 SVVINSLFT-DERMRFEYHRPTKAIALDPDYAKKTSRRFAAGGLAGHLYLNSKKWLGYKD 180
           SVVINSLFT DE+M+F+YHRP KAI+LDPDY KK S+RF AGGLAGHLY+NSKKW G KD
Sbjct: 121 SVVINSLFTDDEKMKFDYHRPMKAISLDPDYTKKQSKRFVAGGLAGHLYMNSKKWFGNKD 180

Query: 181 QVLHSGEGPIHAVKWRTSLIAWANDAGVKVYDAANDQRITFIERPRGSPLPELLLPQLVW 240
           QVLHSGEGPIH+VKWR SLIAWAND GVKVYD A DQR+TFIE+PRGSP PE LLP LVW
Sbjct: 181 QVLHSGEGPIHSVKWRGSLIAWANDVGVKVYDTAKDQRVTFIEKPRGSPRPEALLPHLVW 240

Query: 241 QDDTQLVIGWGTSVKIASIRTSQNKTANGTQNSRHVPMSSMNRVDVVASFQTNYLITGIA 300
           QDDT LVIGWGTSVKIASI++ Q +T       R + MSS+ +VD+VASFQT+Y I+GIA
Sbjct: 241 QDDTLLVIGWGTSVKIASIKSDQQQTG----TFRQIQMSSLTQVDIVASFQTSYYISGIA 300

Query: 301 PFGDVLVVLAYIP-EEEGEKSFSITAP-SRQGNAQRPEIRVVTWNNDELSNDTLPVHGFE 360
           PFGD LV+LAYIP E +GEK FS T   SRQGNAQRPEIR+V+WNNDEL+ D LPVHGFE
Sbjct: 301 PFGDSLVILAYIPIEGDGEKEFSSTTTLSRQGNAQRPEIRIVSWNNDELTMDALPVHGFE 360

Query: 361 HYKAKDYSLAHAPFTGSSYAGGQWASGVEPMYYIVSPKDIVIGKPRDAEDHISWLLEHGW 420
           HYKAKDYSLAHAPF GSSYAGGQWA+G EP+YYIVSPKD+VI KPRDAEDHI+WLL+HG+
Sbjct: 361 HYKAKDYSLAHAPFPGSSYAGGQWAAGDEPLYYIVSPKDVVIAKPRDAEDHINWLLQHGF 420

Query: 421 HEKALEAVEAGQGKSELLDEVGSRYLDHLIVERKYAEAASLCPKLLRGSVSAWERWVFHF 480
           HEKAL AVEA +G++EL+D+VG+ YLDHLIVERKYAEAASLCPKLLRGS SAWERWVFHF
Sbjct: 421 HEKALAAVEASEGRTELIDKVGAGYLDHLIVERKYAEAASLCPKLLRGSASAWERWVFHF 480

Query: 481 AHLCQLPVLAPYIPTENPRLRDTAYEMALVALASNPLFHKDLLTTIKSWPPVIYSALPVI 540
           A L QLPVL PY+PT+NPRL+DT YE+ALVALA+NP +HK+LL+ +KSWP  +YSAL VI
Sbjct: 481 AQLRQLPVLVPYMPTDNPRLKDTVYEVALVALATNPSYHKELLSAVKSWPRSVYSALTVI 540

Query: 541 AAIEPQFNTSSMTDALKEALAELYVIDGQYEKAFLLYADLLKPDIFDFIEKYNLHEAIRE 600
           +AIEPQ NTSSMTDALKEALAELYVIDGQY+KAF LYADLLKP++FDFIEKY+LHEAIR 
Sbjct: 541 SAIEPQLNTSSMTDALKEALAELYVIDGQYQKAFSLYADLLKPEVFDFIEKYSLHEAIRG 600

Query: 601 KVVQLMMLDCKRAVPLFIQYKELIPPNEVVSQL---------FKAVDKCDFRYFMHLYLH 660
           KVVQLM+LDCKRA  LFIQ ++LIPP+EVV QL          KA  KCD RY+++LYLH
Sbjct: 601 KVVQLMLLDCKRATVLFIQNRDLIPPSEVVPQLLKAGKNPQVLKAGKKCDSRYYLYLYLH 660

Query: 661 SLFEVNPHAGKDFHDIQVELYADYDIKMLLPFLRSSQHYTLEKAYDICVKKNLLREQVFI 720
           +LFEV+   GKDFHD+QVELYA+YD KMLLPFLRSSQHY LEKAY++CVKK+ LREQVF+
Sbjct: 661 ALFEVSHDTGKDFHDMQVELYAEYDTKMLLPFLRSSQHYKLEKAYELCVKKDFLREQVFV 720

Query: 721 LGRMGNAKQALAVIIDKLGDIEEAVEFVSMQHDDELWEELIKLCLHKAEMVGMLLEHTVG 780
           LGRMGNAKQALAVII+KLGDIEEAVEFVSMQHDD+LWEELIK CL+K EMVG+LLEHTVG
Sbjct: 721 LGRMGNAKQALAVIINKLGDIEEAVEFVSMQHDDDLWEELIKQCLNKPEMVGLLLEHTVG 780

Query: 781 NLDPLYIVNMVPNGIEIPRLRDRLVKIITDYRTETSLRHGCNDILKADTVNLLVKYYKEA 840
           NLDPLYIVNMVPNG+EIPRLRDRLVKI+TDYRTETSLRHGCNDILK D VNLLVK + EA
Sbjct: 781 NLDPLYIVNMVPNGLEIPRLRDRLVKIVTDYRTETSLRHGCNDILKTDIVNLLVKCFNEA 840

Query: 841 RHGVYLSNEEDEVRGKRNENKVS-----QSIQKSLSVRMMEVKSKTRGGARCCICFNPFS 900
           R GV LS+E+D+ R KR +N  S       + KSLS++M EVKSKTRG  RCC+CF+P S
Sbjct: 841 RRGVCLSHEDDDSRAKREDNNRSSFSQRMVVDKSLSIKMTEVKSKTRGDTRCCMCFDPVS 900

Query: 901 IQNISVIVFFCCHAYHETCLIESTSNLDAKKGTGETQHDSVSDFDYDNGEIEDDDEDEAD 956
           I+  +V+VFFCCHAYHETCL+++  + +  K    T+  S  ++ YDNG  E+++++E D
Sbjct: 901 IRGDTVVVFFCCHAYHETCLMDAAFSNNNHK---TTKGSSGYEYSYDNGVDEEEEDEEED 960