Cp4.1LG20g07410 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g07410 vs. ExPASy Swiss-Prot
Match: Q9SKN1 (Vacuolar fusion protein MON1 homolog OS=Arabidopsis thaliana OX=3702 GN=MON1 PE=1 SV=2)

HSP 1 Score: 661.0 bits (1704), Expect = 1.3e-188
Identity = 369/644 (57.30%), Postives = 435/644 (67.55%), Query Frame = 0

Query: 2   SSGLSSLSSSD-ELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 61
           S   SS SSSD E  + NP + P T  + ++ +L S+ L+ P           + VSDGS
Sbjct: 4   SDSRSSPSSSDTEFADPNPSSDPETNSERVQSQLESMNLSQP-----------SEVSDGS 63

Query: 62  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGT--GVVWGRTN 121
             +  G G    DE      V   N  GN        + G  +REG+ GT  G V  R  
Sbjct: 64  HTEFSGGGDDNDDE------VASAN--GNEGGV----SNGGLLREGVAGTSGGEVLLRAE 123

Query: 122 SEIEV---DRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVH 181
           + +E+   + P SP+SSGY GERGSSG ++   + D  ++DEI+E N D        +  
Sbjct: 124 NPVEMEAGEEPPSPTSSGYDGERGSSGGATSTYKADDGSEDEIREANVDGDTASQHEA-- 183

Query: 182 SWVPGKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQA 241
           +W+PGKRH DE      DDAS SWRKRKKHFF+LS+SGKPIYSRYGDEHKLAGFSA+LQA
Sbjct: 184 AWLPGKRHVDE------DDASTSWRKRKKHFFILSNSGKPIYSRYGDEHKLAGFSATLQA 243

Query: 242 IISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILIL 301
           IISFVE+GGDRV  V+AG H VVFLVKGPIYLVCISCT+E YE LRGQL+L+YGQMILIL
Sbjct: 244 IISFVENGGDRVNLVKAGNHQVVFLVKGPIYLVCISCTDETYEYLRGQLDLLYGQMILIL 303

Query: 302 TKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGA 361
           TKS++RCFE+N KFDMT LLGGTD VFSSL+HSF WNPATFLHAYTCLPL Y  RQA G 
Sbjct: 304 TKSIDRCFEKNAKFDMTPLLGGTDAVFSSLVHSFSWNPATFLHAYTCLPLPYALRQATGT 363

Query: 362 ILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESF---------- 421
           ILQ+V  SG+LF++LMC+HKV+SL GAQKASLHPDD+LLL+NFVMSSESF          
Sbjct: 364 ILQEVCASGVLFSLLMCRHKVVSLAGAQKASLHPDDLLLLSNFVMSSESFRTSESFSPIC 423

Query: 422 ---------------------------------------RIRIETVLLKSNVLSEVQRSM 481
                                                  R+R+E VLLKSN+LS VQRS+
Sbjct: 424 LPRYNAQAFLHAYVHFFDDDTYVILLTTRSDAFHHLKDCRVRLEAVLLKSNILSVVQRSI 483

Query: 482 LDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIY 541
            +GGM VEDVP+D   R R  S    +Q  P        + S G GGP GLWHF+YRSIY
Sbjct: 484 AEGGMRVEDVPID---RRRRSSTTNQEQDSPG------PDISVGTGGPFGLWHFMYRSIY 543

Query: 542 LDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQD 591
           LDQY++SEFS P++S +QQK LYRAYQ +Y SMH K +GPHKTQ+RRDENY LLCWVT D
Sbjct: 544 LDQYISSEFSPPVTSHRQQKSLYRAYQKLYASMHVKGLGPHKTQYRRDENYTLLCWVTPD 603

BLAST of Cp4.1LG20g07410 vs. ExPASy Swiss-Prot
Match: Q5JL08 (Vacuolar fusion protein MON1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=MON1 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 1.4e-171
Identity = 340/637 (53.38%), Postives = 407/637 (63.89%), Query Frame = 0

Query: 13  ELDNFNPRTSPTTPP--KPLEEELASLALT---LPPP-ELLSDQEDVNVVSDGSAADGFG 72
           ++D      SP  PP   P E+ LASL+L    LPP  E+  D +D              
Sbjct: 2   DMDPPTNNPSPPGPPDSPPPEKRLASLSLRTSHLPPDFEIHDDYDD-------------- 61

Query: 73  FGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSEIEVDRP 132
                 D+E  +  V     +  SA+A                    W     + +V  P
Sbjct: 62  -----DDDEGYLTAVSRVGSISTSASA--------------------WKDDLEDADV-AP 121

Query: 133 VSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPGKRHGDE 192
            SPSSSGYA ERG+S ASS        A+D+ Q   DD            W   K+H  E
Sbjct: 122 PSPSSSGYAAERGTSLASS------AAANDDPQPQPDD----------DDWPRDKKHLHE 181

Query: 193 LILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFVEDGGDR 252
                 DD S SWRKRKKHFF+LS+SGKPIYSRYGDEHKLAGFSA+LQAIISFVE+ GD 
Sbjct: 182 ------DDTSASWRKRKKHFFILSNSGKPIYSRYGDEHKLAGFSATLQAIISFVENSGDH 241

Query: 253 VKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVNRCFERN 312
           +K+VRA KH +VFLVKGPIYLVCISCTEE YE LRGQLEL+YGQM+LILTKSVNRCFE+N
Sbjct: 242 IKFVRAAKHQIVFLVKGPIYLVCISCTEESYEGLRGQLELMYGQMLLILTKSVNRCFEKN 301

Query: 313 PKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDVADSGIL 372
           PKFDM  LLGGTD VF SLIH+F WNPATFLHAYTCLPLA  TRQAA A+LQD+ADSG+L
Sbjct: 302 PKFDMAPLLGGTDAVFLSLIHAFSWNPATFLHAYTCLPLAQSTRQAASAVLQDIADSGVL 361

Query: 373 FAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRI------------------- 432
           FA+LMC+HKVISLVGAQKA+LHPDD+ LL+NF++SSESFR                    
Sbjct: 362 FALLMCEHKVISLVGAQKATLHPDDIFLLSNFILSSESFRTSESFSPICLPRYNSMAFLY 421

Query: 433 ------------------------------RIETVLLKSNVLSEVQRSMLDGGMHVEDVP 492
                                         RI+ VLLK+NVL EVQRS+ +  + +ED+P
Sbjct: 422 AYVHFFDENTYLTLLTARSDAFYDLKDSRSRIQNVLLKANVLVEVQRSLRESALRIEDLP 481

Query: 493 VDSLPRYRTISPHLGQQRVPSEFTE----RFKESSAGMGGPGGLWHFIYRSIYLDQYVAS 552
            D  P  +++SP       P +F++    +   S   +GGP GLWHFIY+SIYLDQYV+S
Sbjct: 482 AD--PSSQSVSP-------PPQFSQDLHFQLLSSEMAIGGPAGLWHFIYKSIYLDQYVSS 541

Query: 553 EFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYAAF 591
           EF   IS+ +QQKRLY+AYQ +Y SMHDK  GPHKTQFRRDE+YVL CW+TQDFELYAAF
Sbjct: 542 EFPLIISNPKQQKRLYKAYQKLYASMHDKATGPHKTQFRRDEDYVLFCWITQDFELYAAF 567

BLAST of Cp4.1LG20g07410 vs. ExPASy Swiss-Prot
Match: Q9YGN1 (Protein SAND OS=Takifugu rubripes OX=31033 GN=sand PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 6.1e-50
Identity = 145/471 (30.79%), Postives = 229/471 (48.62%), Query Frame = 0

Query: 137 ERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPGKRHGDELILKVQDDAS 196
           E        G  E  G  +DE+Q L  + ++ +      S  P  R       K +D +S
Sbjct: 62  EAEEKAEQEGVVEDSGQTEDELQVLVGERTITEQHAQDVS--PQSRS------KEEDMSS 121

Query: 197 ISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFVEDGGDRVKWVRAGKHL 256
            +WR  +KH FVLS +GKPIY+RYG E  L+     + A++SFVE   + ++ + A    
Sbjct: 122 EAWRSHRKHVFVLSEAGKPIYTRYGSEEALSSTMGVMMALVSFVESDKNTIRSIHADGCK 181

Query: 257 VVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILT-KSVNRCFERNPKFDMTSLL 316
           V+FL K P+ LV +S T +  + L  +L+ IY Q++ +LT   +N  F+    +D+  LL
Sbjct: 182 VIFLAKSPLVLVGVSQTYQSDKELLRELQYIYYQIVSLLTLTQLNHIFQNKQNYDLRRLL 241

Query: 317 GGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDVADSGILFAILMCKHK 376
            G++ +  +L+H    +P   L A TCLPL+   R    + LQ      ++F+IL+   +
Sbjct: 242 AGSEYLTDNLLHRLDRDPGLLLSAVTCLPLSNSVRDVVSSSLQAAKAKNLVFSILLAGDR 301

Query: 377 VISLVGAQKASLHPDDMLLLANFVMSSESFRIR---IETVLLKSNVLS--EVQRSMLDGG 436
           +++LV  +   LH  D+ L+ N V SS SFR         L K N         S L+  
Sbjct: 302 LVTLVRKKDQFLHHIDLHLVMNLVGSSSSFREGEGWTPICLPKFNTAGFFHAHISYLESA 361

Query: 437 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTER-----FKES------SAGMGGPGGLWH 496
             +  + V S  R    +    +QR     T+R      KE+      S    G   L H
Sbjct: 362 SDLCLILV-STDREDFFNMSDCKQRFLERLTKRTAYQALKEALKCPSYSVEQVGIPELRH 421

Query: 497 FIYRSIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVL 556
           F+Y+S     Y + EF     S ++Q+RL   YQ+++  +H   + P +  +R      L
Sbjct: 422 FLYKSKSSGLYTSPEFPELYQSDEEQERLMGLYQDLHSHLH-HPVRPLRFFYRCTNKENL 481

Query: 557 LCWVTQDFELYAAFDPLADKAMAIKICNRVCQWIKDVENEVFLLGASPFSW 591
           L WVT  F+LY  F PL  KAMA+   N++ +WI+  E+ +F+L  SP ++
Sbjct: 482 LAWVTNGFQLYLCFSPLGTKAMAVSAVNKLLKWIRKEEDRLFIL--SPLTY 520

BLAST of Cp4.1LG20g07410 vs. ExPASy Swiss-Prot
Match: Q86VX9 (Vacuolar fusion protein MON1 homolog A OS=Homo sapiens OX=9606 GN=MON1A PE=1 SV=3)

HSP 1 Score: 197.6 bits (501), Expect = 4.0e-49
Identity = 126/427 (29.51%), Postives = 209/427 (48.95%), Query Frame = 0

Query: 180 GKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 239
           G   GDE      +DA+ +WR  +KH FVLS +GKP+YSRYG E  L+     + A++SF
Sbjct: 235 GTTEGDE------EDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSF 294

Query: 240 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILT-KS 299
           +E   + ++ + A  + VVF+ + P+ LV ++ T +  + L  +L  IY Q++ +LT   
Sbjct: 295 LEADKNAIRSIHADGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQ 354

Query: 300 VNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQ 359
           ++  F++   +D+  LL G++ +  +L+     +P+  + A  CLPLA   R    A LQ
Sbjct: 355 LSHIFQQKQNYDLRRLLSGSERITDNLLQLMARDPSFLMGAARCLPLAAAVRDTVSASLQ 414

Query: 360 DVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR---IRIETVLLK 419
                 ++F+IL+ ++++++LV  +   LHP D+ LL N + SS SFR         L K
Sbjct: 415 QARARSLVFSILLARNQLVALVRRKDQFLHPIDLHLLFNLISSSSSFREGEAWTPVCLPK 474

Query: 420 SNVLSEVQRSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKES-------- 479
            N              H+  +  D+      +S         S+   RF+E         
Sbjct: 475 FNAAGFFH-------AHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHL 534

Query: 480 -----------SAGMGGPGGLWHFIYRSIYLDQYVASEFSSPISSRQQQKRLYRAYQNIY 539
                      S    G   L HF+Y+S     + + E  +P +S ++Q+RL   YQ ++
Sbjct: 535 ALREALRTPYYSVAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLH 594

Query: 540 DSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYAAFDPLADKAMAIKICNRVCQWIKDV 584
              H+    P KT +    N  LL WVT  FELY  + PL  KA A+   +++ +WI+  
Sbjct: 595 SRAHNAS-RPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKE 647

BLAST of Cp4.1LG20g07410 vs. ExPASy Swiss-Prot
Match: Q6PDG8 (Vacuolar fusion protein MON1 homolog A OS=Mus musculus OX=10090 GN=Mon1a PE=1 SV=3)

HSP 1 Score: 197.6 bits (501), Expect = 4.0e-49
Identity = 124/415 (29.88%), Postives = 205/415 (49.40%), Query Frame = 0

Query: 192 QDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFVEDGGDRVKWVR 251
           ++DA+ +WR  +KH FVLS +GKP+YSRYG E  L+     + A++SF+E   + ++ + 
Sbjct: 145 EEDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIH 204

Query: 252 AGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILT-KSVNRCFERNPKFD 311
           A  + VVF+ + P+ LV ++ T +  + L  +L  IY Q++ +LT   ++  F++   +D
Sbjct: 205 ADGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYD 264

Query: 312 MTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDVADSGILFAIL 371
           +  LL G++ +  +L+     +P+  + A  CLPLA   R    A LQ      ++F+IL
Sbjct: 265 LRRLLSGSERITDNLLQLMARDPSFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSIL 324

Query: 372 MCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR---IRIETVLLKSNVLSEVQRSML 431
           +  +++++LV  +   LHP D+ LL N + SS SFR         L K N          
Sbjct: 325 LAHNQLVALVRRKDQFLHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFH---- 384

Query: 432 DGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKES-------------------S 491
               H+  +  D+      IS         S+   RF+E                    S
Sbjct: 385 ---AHISYLEPDTDLCLLLISTDREDFFAVSDCRRRFQERLRKRGTHLALREALRTPYYS 444

Query: 492 AGMGGPGGLWHFIYRSIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHK 551
               G   L HF+Y+S     + + E  +P SS ++Q+RL   YQ ++   H+    P K
Sbjct: 445 VAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYSSEEEQERLLGLYQYLHSRAHNAS-RPLK 504

Query: 552 TQFRRDENYVLLCWVTQDFELYAAFDPLADKAMAIKICNRVCQWIKDVENEVFLL 584
           T +    N  LL WVT  FELY  + PL  KA A+   +++ +WI+  E+ +F+L
Sbjct: 505 TIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEEDRLFIL 551

BLAST of Cp4.1LG20g07410 vs. NCBI nr
Match: XP_023520062.1 (vacuolar fusion protein MON1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1137 bits (2941), Expect = 0.0
Identity = 584/639 (91.39%), Postives = 584/639 (91.39%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120
           AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE
Sbjct: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120

Query: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPG 180
           IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPG
Sbjct: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPG 180

Query: 181 KRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240
           KRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV
Sbjct: 181 KRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240

Query: 241 EDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300
           EDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN
Sbjct: 241 EDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300

Query: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360
           RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV
Sbjct: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360

Query: 361 ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR-------------- 420
           ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR              
Sbjct: 361 ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFSPICLPRYN 420

Query: 421 -----------------------------------IRIETVLLKSNVLSEVQRSMLDGGM 480
                                              IRIETVLLKSNVLSEVQRSMLDGGM
Sbjct: 421 PMAFLYAYVHYFDANTYFMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQRSMLDGGM 480

Query: 481 HVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYV 540
           HVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYV
Sbjct: 481 HVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYV 540

Query: 541 ASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 590
           ASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA
Sbjct: 541 ASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 600

BLAST of Cp4.1LG20g07410 vs. NCBI nr
Match: KAG6583584.1 (Vacuolar fusion protein MON1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1127 bits (2915), Expect = 0.0
Identity = 582/640 (90.94%), Postives = 582/640 (90.94%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLP PELLSDQEDVNVVSDGS
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPRPELLSDQEDVNVVSDGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVV-EGATGNSVREGIDGTGVVWGRTNS 120
           AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVV EG TGNSVREGIDGTGVVWGRTNS
Sbjct: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVVEGETGNSVREGIDGTGVVWGRTNS 120

Query: 121 EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVP 180
           EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVP
Sbjct: 121 EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVP 180

Query: 181 GKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 240
           GKRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF
Sbjct: 181 GKRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 240

Query: 241 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV 300
           VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV
Sbjct: 241 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV 300

Query: 301 NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD 360
           NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD
Sbjct: 301 NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD 360

Query: 361 VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR------------- 420
           VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR             
Sbjct: 361 VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFSPICLPRY 420

Query: 421 ------------------------------------IRIETVLLKSNVLSEVQRSMLDGG 480
                                               IRIETVLLKSNVLSEVQRSMLDGG
Sbjct: 421 NPMAFLYAYVHYFDANTYFMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQRSMLDGG 480

Query: 481 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY 540
           MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY
Sbjct: 481 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY 540

Query: 541 VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY 590
           VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY
Sbjct: 541 VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY 600

BLAST of Cp4.1LG20g07410 vs. NCBI nr
Match: XP_022964973.1 (vacuolar fusion protein MON1 homolog [Cucurbita moschata])

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 581/640 (90.78%), Postives = 581/640 (90.78%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLP PELLSDQEDVNVVSDGS
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPRPELLSDQEDVNVVSDGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVV-EGATGNSVREGIDGTGVVWGRTNS 120
           AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVV EG TGNSVREGIDGTGVVWGRTNS
Sbjct: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVVEGETGNSVREGIDGTGVVWGRTNS 120

Query: 121 EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVP 180
           EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDD SVGDNSNSVHSWVP
Sbjct: 121 EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDVSVGDNSNSVHSWVP 180

Query: 181 GKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 240
           GKRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF
Sbjct: 181 GKRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 240

Query: 241 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV 300
           VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV
Sbjct: 241 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV 300

Query: 301 NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD 360
           NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD
Sbjct: 301 NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD 360

Query: 361 VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR------------- 420
           VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR             
Sbjct: 361 VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFSPICLPRY 420

Query: 421 ------------------------------------IRIETVLLKSNVLSEVQRSMLDGG 480
                                               IRIETVLLKSNVLSEVQRSMLDGG
Sbjct: 421 NPMAFLYAYVHYFDANTYFMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQRSMLDGG 480

Query: 481 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY 540
           MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY
Sbjct: 481 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY 540

Query: 541 VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY 590
           VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY
Sbjct: 541 VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY 600

BLAST of Cp4.1LG20g07410 vs. NCBI nr
Match: XP_022970304.1 (vacuolar fusion protein MON1 homolog [Cucurbita maxima])

HSP 1 Score: 1113 bits (2878), Expect = 0.0
Identity = 577/647 (89.18%), Postives = 578/647 (89.34%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRT PTTPPKPLEEELASLALTLP PELLSDQEDVNVVSDGS
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTLPTTPPKPLEEELASLALTLPRPELLSDQEDVNVVSDGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120
           AA GFGFGIQRSDEESR GVVCEENVVGNSAAAVVEGATGNSV EGIDGTGV+WGRTNSE
Sbjct: 61  AAVGFGFGIQRSDEESRAGVVCEENVVGNSAAAVVEGATGNSVWEGIDGTGVMWGRTNSE 120

Query: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDA--------SVGDNSN 180
           IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDD         SVGDNSN
Sbjct: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDEIQELNDDDSVGDNSN 180

Query: 181 SVHSWVPGKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSAS 240
           SVHSWVPGKRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSAS
Sbjct: 181 SVHSWVPGKRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSAS 240

Query: 241 LQAIISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMI 300
           LQAIISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMI
Sbjct: 241 LQAIISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMI 300

Query: 301 LILTKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQA 360
           LILTKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQA
Sbjct: 301 LILTKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQA 360

Query: 361 AGAILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR------ 420
           AGAILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR      
Sbjct: 361 AGAILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFS 420

Query: 421 -------------------------------------------IRIETVLLKSNVLSEVQ 480
                                                      IRIETVLLKSNVLSEVQ
Sbjct: 421 PICLPRYNPMAFLYAYVHYFDANTYFMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQ 480

Query: 481 RSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYR 540
           RSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYR
Sbjct: 481 RSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYR 540

Query: 541 SIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWV 590
           SIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWV
Sbjct: 541 SIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWV 600

BLAST of Cp4.1LG20g07410 vs. NCBI nr
Match: XP_004139448.1 (vacuolar fusion protein MON1 homolog [Cucumis sativus] >KAE8652778.1 hypothetical protein Csa_022725 [Cucumis sativus])

HSP 1 Score: 1026 bits (2654), Expect = 0.0
Identity = 536/639 (83.88%), Postives = 552/639 (86.38%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRTSP+TPPKPLEEELASLALTLP P+LLSDQ+DVN VSDG 
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTSPSTPPKPLEEELASLALTLPHPQLLSDQDDVNGVSDGF 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120
            AD   FGIQRSDEE RVG+V EE+VVGNS   VVEGAT     E  DG GVVWGRTNSE
Sbjct: 61  EADSSMFGIQRSDEEMRVGLVSEEHVVGNSVP-VVEGAT-----EVSDGAGVVWGRTNSE 120

Query: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPG 180
           IEVDRP SPSSSGYAGERGSS ASSGRSE DG+A+DEIQEL DDASVG+NSNSV SWVPG
Sbjct: 121 IEVDRPASPSSSGYAGERGSSSASSGRSETDGVAEDEIQELRDDASVGENSNSVPSWVPG 180

Query: 181 KRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240
           KRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV
Sbjct: 181 KRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240

Query: 241 EDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300
           EDGGDRVKWVRAGKH VVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN
Sbjct: 241 EDGGDRVKWVRAGKHQVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300

Query: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360
           RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV
Sbjct: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360

Query: 361 ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR-------------- 420
           ADSGILFAILMCKHKVIS+VGAQKASLHPDDMLLLANFVMS+ESFR              
Sbjct: 361 ADSGILFAILMCKHKVISIVGAQKASLHPDDMLLLANFVMSTESFRTSESFSPICLPRYN 420

Query: 421 -----------------------------------IRIETVLLKSNVLSEVQRSMLDGGM 480
                                              IRIETVLLKS+VLSEVQRSMLDGGM
Sbjct: 421 PMAFLYAYVHYFDVNTYLMLLTTNSDSFYHLKECRIRIETVLLKSHVLSEVQRSMLDGGM 480

Query: 481 HVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYV 540
           HV DVPVDS+PRYRT S HLGQQR PSEF ERFKES+AGMGGPGGLWHFIYRSIYLDQYV
Sbjct: 481 HVGDVPVDSVPRYRTTS-HLGQQRAPSEFPERFKESNAGMGGPGGLWHFIYRSIYLDQYV 540

Query: 541 ASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 590
           +SEFSSPISSRQQQKRLYRAYQNIY SMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA
Sbjct: 541 SSEFSSPISSRQQQKRLYRAYQNIYASMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 600

BLAST of Cp4.1LG20g07410 vs. ExPASy TrEMBL
Match: A0A6J1HMH2 (Vacuolar fusion protein MON1 homolog OS=Cucurbita moschata OX=3662 GN=LOC111464922 PE=3 SV=1)

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 581/640 (90.78%), Postives = 581/640 (90.78%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLP PELLSDQEDVNVVSDGS
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPRPELLSDQEDVNVVSDGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVV-EGATGNSVREGIDGTGVVWGRTNS 120
           AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVV EG TGNSVREGIDGTGVVWGRTNS
Sbjct: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVVEGETGNSVREGIDGTGVVWGRTNS 120

Query: 121 EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVP 180
           EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDD SVGDNSNSVHSWVP
Sbjct: 121 EIEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDVSVGDNSNSVHSWVP 180

Query: 181 GKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 240
           GKRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF
Sbjct: 181 GKRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISF 240

Query: 241 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV 300
           VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV
Sbjct: 241 VEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSV 300

Query: 301 NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD 360
           NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD
Sbjct: 301 NRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQD 360

Query: 361 VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR------------- 420
           VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR             
Sbjct: 361 VADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFSPICLPRY 420

Query: 421 ------------------------------------IRIETVLLKSNVLSEVQRSMLDGG 480
                                               IRIETVLLKSNVLSEVQRSMLDGG
Sbjct: 421 NPMAFLYAYVHYFDANTYFMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQRSMLDGG 480

Query: 481 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY 540
           MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY
Sbjct: 481 MHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQY 540

Query: 541 VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY 590
           VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY
Sbjct: 541 VASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELY 600

BLAST of Cp4.1LG20g07410 vs. ExPASy TrEMBL
Match: A0A6J1I552 (Vacuolar fusion protein MON1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469307 PE=3 SV=1)

HSP 1 Score: 1113 bits (2878), Expect = 0.0
Identity = 577/647 (89.18%), Postives = 578/647 (89.34%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELDNFNPRT PTTPPKPLEEELASLALTLP PELLSDQEDVNVVSDGS
Sbjct: 1   MSSGLSSLSSSDELDNFNPRTLPTTPPKPLEEELASLALTLPRPELLSDQEDVNVVSDGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120
           AA GFGFGIQRSDEESR GVVCEENVVGNSAAAVVEGATGNSV EGIDGTGV+WGRTNSE
Sbjct: 61  AAVGFGFGIQRSDEESRAGVVCEENVVGNSAAAVVEGATGNSVWEGIDGTGVMWGRTNSE 120

Query: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDA--------SVGDNSN 180
           IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDD         SVGDNSN
Sbjct: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDEIQELNDDDSVGDNSN 180

Query: 181 SVHSWVPGKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSAS 240
           SVHSWVPGKRHGDE      DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSAS
Sbjct: 181 SVHSWVPGKRHGDE------DDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSAS 240

Query: 241 LQAIISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMI 300
           LQAIISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMI
Sbjct: 241 LQAIISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMI 300

Query: 301 LILTKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQA 360
           LILTKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQA
Sbjct: 301 LILTKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQA 360

Query: 361 AGAILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR------ 420
           AGAILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR      
Sbjct: 361 AGAILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFS 420

Query: 421 -------------------------------------------IRIETVLLKSNVLSEVQ 480
                                                      IRIETVLLKSNVLSEVQ
Sbjct: 421 PICLPRYNPMAFLYAYVHYFDANTYFMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQ 480

Query: 481 RSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYR 540
           RSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYR
Sbjct: 481 RSMLDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYR 540

Query: 541 SIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWV 590
           SIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWV
Sbjct: 541 SIYLDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWV 600

BLAST of Cp4.1LG20g07410 vs. ExPASy TrEMBL
Match: A0A1S3CIR5 (Vacuolar fusion protein MON1 homolog OS=Cucumis melo OX=3656 GN=LOC103500868 PE=3 SV=1)

HSP 1 Score: 1023 bits (2645), Expect = 0.0
Identity = 534/639 (83.57%), Postives = 552/639 (86.38%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MS GLSSLSSSDELD+FNPRTSP+TPPKPLEEELASLALTLP P+LLSDQ+DVN VSDG 
Sbjct: 1   MSCGLSSLSSSDELDDFNPRTSPSTPPKPLEEELASLALTLPHPQLLSDQDDVNGVSDGF 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120
            AD   FGIQ+SDEE RVG+V EE+V GNS + VVEGAT     EG DG GVVWGRTNSE
Sbjct: 61  EADRSMFGIQQSDEEMRVGLVFEEHVGGNSVS-VVEGAT-----EGSDGAGVVWGRTNSE 120

Query: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPG 180
           IEVDRP SPSSSGYAGERGSS ASSGRSEMDG+A+DEIQEL DDAS G+NSNSV SWVPG
Sbjct: 121 IEVDRPASPSSSGYAGERGSSSASSGRSEMDGVAEDEIQELRDDASDGENSNSVPSWVPG 180

Query: 181 KRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240
           KRHGDE      DDASISWRKRKKHFF+LSHSGKPIYSRYGDEHKLAGFSASLQAIISFV
Sbjct: 181 KRHGDE------DDASISWRKRKKHFFILSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240

Query: 241 EDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300
           EDGGDRVKWVRAGKH VVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN
Sbjct: 241 EDGGDRVKWVRAGKHQVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300

Query: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360
           RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV
Sbjct: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360

Query: 361 ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR-------------- 420
           ADSGILFAILMCKHKVIS+VGAQKASLHPDDMLLLANFVMS+ESFR              
Sbjct: 361 ADSGILFAILMCKHKVISIVGAQKASLHPDDMLLLANFVMSTESFRTSESFSPICLPRYN 420

Query: 421 -----------------------------------IRIETVLLKSNVLSEVQRSMLDGGM 480
                                              IRIETVLLKSNVLSEVQRSMLDGGM
Sbjct: 421 PMAFLYAYVHYFDVNTYLMLLTTNSDSFYHLKECRIRIETVLLKSNVLSEVQRSMLDGGM 480

Query: 481 HVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYV 540
           HV DVPVDSLPRYRT S HLGQQR PSEF ERFKES+AGMGGPGGLWHFIYRSIYLDQYV
Sbjct: 481 HVGDVPVDSLPRYRTTS-HLGQQRAPSEFPERFKESNAGMGGPGGLWHFIYRSIYLDQYV 540

Query: 541 ASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 590
           +SEFSSPI+SRQQQKRLYRAYQNIY SMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA
Sbjct: 541 SSEFSSPITSRQQQKRLYRAYQNIYASMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 600

BLAST of Cp4.1LG20g07410 vs. ExPASy TrEMBL
Match: A0A6J1DFB6 (Vacuolar fusion protein MON1 homolog OS=Momordica charantia OX=3673 GN=LOC111020536 PE=3 SV=1)

HSP 1 Score: 985 bits (2546), Expect = 0.0
Identity = 517/639 (80.91%), Postives = 535/639 (83.72%), Query Frame = 0

Query: 1   MSSGLSSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 60
           MSSGLSSLSSSDELD FNP+ +PT PPKPLEEELASLALTLP PELLSD++DVN V +GS
Sbjct: 1   MSSGLSSLSSSDELDEFNPKPTPTAPPKPLEEELASLALTLPHPELLSDRQDVNGVPNGS 60

Query: 61  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSE 120
            ADG  FGIQ++D E R G V EENVVGNS +         SVR GI G GVVWGRTNSE
Sbjct: 61  PADGSQFGIQQNDGEMRDGAVPEENVVGNSES---------SVRGGIGGAGVVWGRTNSE 120

Query: 121 IEVDRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPG 180
           IEVDRP SPSSSGYAGERGSS ASSGRSEMDGIA+DEIQEL DDASVG    S+ SWVPG
Sbjct: 121 IEVDRPASPSSSGYAGERGSSSASSGRSEMDGIAEDEIQELRDDASVG---VSLPSWVPG 180

Query: 181 KRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240
           KRHG+E      DDASISWRKRKKHFF+LSHSGKPIYSRYGDEHKLAGFSASLQAIISFV
Sbjct: 181 KRHGNE------DDASISWRKRKKHFFILSHSGKPIYSRYGDEHKLAGFSASLQAIISFV 240

Query: 241 EDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300
           EDGGDRVK VRAGKH VVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN
Sbjct: 241 EDGGDRVKLVRAGKHQVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVN 300

Query: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDV 360
           RCFERNPKFDMTSLLGGTDVVFSSLIHSF WNPATFLHAYTCL LAYGTRQAAGAILQDV
Sbjct: 301 RCFERNPKFDMTSLLGGTDVVFSSLIHSFSWNPATFLHAYTCLXLAYGTRQAAGAILQDV 360

Query: 361 ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR-------------- 420
           ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR              
Sbjct: 361 ADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFRTSESFSPICLPRYN 420

Query: 421 -----------------------------------IRIETVLLKSNVLSEVQRSMLDGGM 480
                                              IRIE+VLLKSNVLSEVQRSMLDGGM
Sbjct: 421 PMAFLYAYVHYFDVNTYLMLLTTNSDSFYHLKECRIRIESVLLKSNVLSEVQRSMLDGGM 480

Query: 481 HVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYV 540
            VED+PVDS PRYRTISPHLGQ R PSE  ERFKES AG+GGPGGLWHFIYRSIYLDQYV
Sbjct: 481 RVEDMPVDSSPRYRTISPHLGQHRAPSELPERFKESYAGIGGPGGLWHFIYRSIYLDQYV 540

Query: 541 ASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 590
           +SEFSSPISS  QQKRLYRAYQNIY SMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA
Sbjct: 541 SSEFSSPISSHHQQKRLYRAYQNIYASMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYA 600

BLAST of Cp4.1LG20g07410 vs. ExPASy TrEMBL
Match: A0A660KT03 (Vacuolar fusion protein MON1 homolog OS=Carpinus fangiana OX=176857 GN=FH972_011312 PE=3 SV=1)

HSP 1 Score: 812 bits (2098), Expect = 1.53e-290
Identity = 432/634 (68.14%), Postives = 477/634 (75.24%), Query Frame = 0

Query: 6   SSLSSSDELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGSAADGF 65
           S  +S D+L + NP  +PT  PKPLE+E ASLAL  P  E    QE +N V++G  A+  
Sbjct: 3   SDSTSGDDLPDPNPNPTPTPTPKPLEDEFASLALAEPHNEAPEGQEALNGVANGPLAENN 62

Query: 66  GFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGTGVVWGRTNSEIEVDR 125
              I+  + E  V  V EE +  N              RE +   GVVW RTNSE+EVD 
Sbjct: 63  HREIESGEREGEVSSV-EERIEINLEV--------EEARESVQ-RGVVWRRTNSEVEVDG 122

Query: 126 PVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVHSWVPGKRHGD 185
             SPSSSGYAGERGS+ ASSG S +DGI +DEIQE+ ++ S+   S+S  +WVPGKRH D
Sbjct: 123 QSSPSSSGYAGERGSTSASSGGSGIDGIGEDEIQEVENEGSISGFSDSPATWVPGKRHLD 182

Query: 186 ELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQAIISFVEDGGD 245
           E      DDASISWRKRKKHFF+LS+SGKPIYSRYGDEHKLAGFSA+LQAIISFVE+GGD
Sbjct: 183 E------DDASISWRKRKKHFFILSNSGKPIYSRYGDEHKLAGFSATLQAIISFVENGGD 242

Query: 246 RVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILILTKSVNRCFER 305
           RVK VRAGKH VVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQM++ILTKSVNRCFE+
Sbjct: 243 RVKLVRAGKHQVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMLVILTKSVNRCFEK 302

Query: 306 NPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGAILQDVADSGI 365
           NPKFDMT LLGGTDVVFSSLIHSF WNPATFLHAYTCLPLAY TRQAAGAILQDVADSG+
Sbjct: 303 NPKFDMTPLLGGTDVVFSSLIHSFSWNPATFLHAYTCLPLAYATRQAAGAILQDVADSGV 362

Query: 366 LFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESFR------------------- 425
           LFAILMCKHKV+SLVGAQKASLHPDDMLLLANFVMSSESFR                   
Sbjct: 363 LFAILMCKHKVVSLVGAQKASLHPDDMLLLANFVMSSESFRTSESFSPICLPRYNPMAFL 422

Query: 426 ------------------------------IRIETVLLKSNVLSEVQRSMLDGGMHVEDV 485
                                         IRIE VLLKSNVLSEVQRSMLDGGMHVED+
Sbjct: 423 YSYVHYFDVDTYLMLLTTSSDAFYHLKDCRIRIEVVLLKSNVLSEVQRSMLDGGMHVEDL 482

Query: 486 PVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIYLDQYVASEFS 545
           P D LPR   +SPHLGQ   P +  E F++S  G+GGP GLWHFIYRSIYLDQYV+SEFS
Sbjct: 483 PTDPLPRSGYVSPHLGQ---PRDSPEGFRQSYVGIGGPAGLWHFIYRSIYLDQYVSSEFS 542

Query: 546 SPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQDFELYAAFDPL 590
             I S QQQKRLYRAYQ +Y SMH+  IGPHKTQFRRDENYVLLCWVTQDFELYAAFDPL
Sbjct: 543 PAIGSSQQQKRLYRAYQKLYASMHNSGIGPHKTQFRRDENYVLLCWVTQDFELYAAFDPL 602

BLAST of Cp4.1LG20g07410 vs. TAIR 10
Match: AT2G28390.1 (SAND family protein )

HSP 1 Score: 661.0 bits (1704), Expect = 9.0e-190
Identity = 369/644 (57.30%), Postives = 435/644 (67.55%), Query Frame = 0

Query: 2   SSGLSSLSSSD-ELDNFNPRTSPTTPPKPLEEELASLALTLPPPELLSDQEDVNVVSDGS 61
           S   SS SSSD E  + NP + P T  + ++ +L S+ L+ P           + VSDGS
Sbjct: 4   SDSRSSPSSSDTEFADPNPSSDPETNSERVQSQLESMNLSQP-----------SEVSDGS 63

Query: 62  AADGFGFGIQRSDEESRVGVVCEENVVGNSAAAVVEGATGNSVREGIDGT--GVVWGRTN 121
             +  G G    DE      V   N  GN        + G  +REG+ GT  G V  R  
Sbjct: 64  HTEFSGGGDDNDDE------VASAN--GNEGGV----SNGGLLREGVAGTSGGEVLLRAE 123

Query: 122 SEIEV---DRPVSPSSSGYAGERGSSGASSGRSEMDGIADDEIQELNDDASVGDNSNSVH 181
           + +E+   + P SP+SSGY GERGSSG ++   + D  ++DEI+E N D        +  
Sbjct: 124 NPVEMEAGEEPPSPTSSGYDGERGSSGGATSTYKADDGSEDEIREANVDGDTASQHEA-- 183

Query: 182 SWVPGKRHGDELILKVQDDASISWRKRKKHFFVLSHSGKPIYSRYGDEHKLAGFSASLQA 241
           +W+PGKRH DE      DDAS SWRKRKKHFF+LS+SGKPIYSRYGDEHKLAGFSA+LQA
Sbjct: 184 AWLPGKRHVDE------DDASTSWRKRKKHFFILSNSGKPIYSRYGDEHKLAGFSATLQA 243

Query: 242 IISFVEDGGDRVKWVRAGKHLVVFLVKGPIYLVCISCTEEPYESLRGQLELIYGQMILIL 301
           IISFVE+GGDRV  V+AG H VVFLVKGPIYLVCISCT+E YE LRGQL+L+YGQMILIL
Sbjct: 244 IISFVENGGDRVNLVKAGNHQVVFLVKGPIYLVCISCTDETYEYLRGQLDLLYGQMILIL 303

Query: 302 TKSVNRCFERNPKFDMTSLLGGTDVVFSSLIHSFGWNPATFLHAYTCLPLAYGTRQAAGA 361
           TKS++RCFE+N KFDMT LLGGTD VFSSL+HSF WNPATFLHAYTCLPL Y  RQA G 
Sbjct: 304 TKSIDRCFEKNAKFDMTPLLGGTDAVFSSLVHSFSWNPATFLHAYTCLPLPYALRQATGT 363

Query: 362 ILQDVADSGILFAILMCKHKVISLVGAQKASLHPDDMLLLANFVMSSESF---------- 421
           ILQ+V  SG+LF++LMC+HKV+SL GAQKASLHPDD+LLL+NFVMSSESF          
Sbjct: 364 ILQEVCASGVLFSLLMCRHKVVSLAGAQKASLHPDDLLLLSNFVMSSESFRTSESFSPIC 423

Query: 422 ---------------------------------------RIRIETVLLKSNVLSEVQRSM 481
                                                  R+R+E VLLKSN+LS VQRS+
Sbjct: 424 LPRYNAQAFLHAYVHFFDDDTYVILLTTRSDAFHHLKDCRVRLEAVLLKSNILSVVQRSI 483

Query: 482 LDGGMHVEDVPVDSLPRYRTISPHLGQQRVPSEFTERFKESSAGMGGPGGLWHFIYRSIY 541
            +GGM VEDVP+D   R R  S    +Q  P        + S G GGP GLWHF+YRSIY
Sbjct: 484 AEGGMRVEDVPID---RRRRSSTTNQEQDSPG------PDISVGTGGPFGLWHFMYRSIY 543

Query: 542 LDQYVASEFSSPISSRQQQKRLYRAYQNIYDSMHDKEIGPHKTQFRRDENYVLLCWVTQD 591
           LDQY++SEFS P++S +QQK LYRAYQ +Y SMH K +GPHKTQ+RRDENY LLCWVT D
Sbjct: 544 LDQYISSEFSPPVTSHRQQKSLYRAYQKLYASMHVKGLGPHKTQYRRDENYTLLCWVTPD 603