Cp4.1LG20g07300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g07300
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Carbonic anhydrase
Location	Cp4.1LG20: 6278872 .. 6280315 (+)
RNA-Seq Expression	Cp4.1LG20g07300
Synteny	Cp4.1LG20g07300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGCTCTCTTTTCATGCGTTGTTTTGCACATTTTTGTGTGCCTTCCTTTTGGTTTCATGGCGTGCAATGTCTCAAGAAGTTGGTAGGCAACCTATTGTGTCATGTAGTTTATTAGGATATGAGCTTTGTTGTATAATATTTAAATGTTTGTTTGATATTGCATGCAGAGGATCAGAAGGAGTTTGATTATAATGAAGAGGGAAAGAGAGGGCCTTCCCATTGGGGAGATCTTAAGAGAGAGTGGCATAGCTGCAAGGCTGGGCTAATGCAATCTCCCATTGATCTATTGCACGAGCGAGTTCGAATTGTACCCCATTTTACGAACCTCAAGACCGAGTATAAGCCTTCTAATGCAACTCTTAAGAACCGAGGCCATGATATTATGGTAATTGTTGTAATGTCAACTTTGCTTATAATTATGTTTAAACAAAACCCTTTTGAGTATGGATTGTGATGGTTATTGTAGTTGAAATGGGGTGAAGGGGCTGGCTATATGGAAGTTAATGGAACTCGCTACTTTCTTAAGCAATTCCATTGGCATTCGCCCTCTGAGCATACAATCAATGGCAGAAGATTTGCTTTAGAAGCCCATTTGGTGCACCAAAGCCCAACAGGAAGTGTTGCTGTCATTGGTGTTCTCTACAAAATTGGACGACCTGACTATTTCTTGTCCAAGGTAAATGTTCAAATATCAATTTTTGAAGATGAGACTGATTAAGATTGTTATGTTCTAAATTTAACACTTTTTTTTTTTTCTTCACTTAGATGAAAGAGTATTTGGAAGAAGTTTCTGATACAAAAGAAGATGAACCAATTGATTTGGCTGATCCGTCACAATTAGAGATGAGAAGCAGTTTATATTATCGATATATTGGCTCTCTAACTGTTCCGCCGTGTTCTCAAAATGTGCTTTGGACCCTTGTCAGAAAGGTATTTTCTTTTTCAAGTTAGATCATCCTATGTATATTCATGGTTTTAATGCTTAAATCATTTTTATACGTTTTCAGGTTAGGACCGTTTCACCTGAACAAGTGAAGTTACTTCGAGTGGCGGTTCATGATGTAAGTAATATAATTTTATGTCAATGGACAATTTAGTTCTTAAATGAGGTTTGGAATTTGTTATGCATGCATTTAAACTTTTATTTGATCCTATCGAATTTTTGTTCATGTATTTTTCATATTCGTATTTTGATTTGTATTCATATACCTCTAATTTTTAATTGTGTAATTAGGGTTTGTGTACTTGGTTCATTTATGAAACCACGATAATTTTCTCCACTCAATCATCTAATTTTGAGCGGATTCAATTACTTGCTCTCATATTAGCATATTAGAGCGGTTTTAATCGGTTTATATATATGCAGGACTCAGACACTAATGCAAGGCCTTTGCAACCGCTTAATGATCGAAAGATTCAACTCCGAGTCAAATCATGA

mRNA sequence

ATGGAGAAGCTCTCTTTTCATGCGTTGTTTTGCACATTTTTGTGTGCCTTCCTTTTGGTTTCATGGCGTGCAATGTCTCAAGAAGTTGAGGATCAGAAGGAGTTTGATTATAATGAAGAGGGAAAGAGAGGGCCTTCCCATTGGGGAGATCTTAAGAGAGAGTGGCATAGCTGCAAGGCTGGGCTAATGCAATCTCCCATTGATCTATTGCACGAGCGAGTTCGAATTGTACCCCATTTTACGAACCTCAAGACCGAGTATAAGCCTTCTAATGCAACTCTTAAGAACCGAGGCCATGATATTATGTTGAAATGGGGTGAAGGGGCTGGCTATATGGAAGTTAATGGAACTCGCTACTTTCTTAAGCAATTCCATTGGCATTCGCCCTCTGAGCATACAATCAATGGCAGAAGATTTGCTTTAGAAGCCCATTTGGTGCACCAAAGCCCAACAGGAAGTGTTGCTGTCATTGGTGTTCTCTACAAAATTGGACGACCTGACTATTTCTTGTCCAAGATGAAAGAGTATTTGGAAGAAGTTTCTGATACAAAAGAAGATGAACCAATTGATTTGGCTGATCCGTCACAATTAGAGATGAGAAGCAGTTTATATTATCGATATATTGGCTCTCTAACTGTTCCGCCGTGTTCTCAAAATGTGCTTTGGACCCTTGTCAGAAAGGTTAGGACCGTTTCACCTGAACAAGTGAAGTTACTTCGAGTGGCGGTTCATGATGACTCAGACACTAATGCAAGGCCTTTGCAACCGCTTAATGATCGAAAGATTCAACTCCGAGTCAAATCATGA

Coding sequence (CDS)

ATGGAGAAGCTCTCTTTTCATGCGTTGTTTTGCACATTTTTGTGTGCCTTCCTTTTGGTTTCATGGCGTGCAATGTCTCAAGAAGTTGAGGATCAGAAGGAGTTTGATTATAATGAAGAGGGAAAGAGAGGGCCTTCCCATTGGGGAGATCTTAAGAGAGAGTGGCATAGCTGCAAGGCTGGGCTAATGCAATCTCCCATTGATCTATTGCACGAGCGAGTTCGAATTGTACCCCATTTTACGAACCTCAAGACCGAGTATAAGCCTTCTAATGCAACTCTTAAGAACCGAGGCCATGATATTATGTTGAAATGGGGTGAAGGGGCTGGCTATATGGAAGTTAATGGAACTCGCTACTTTCTTAAGCAATTCCATTGGCATTCGCCCTCTGAGCATACAATCAATGGCAGAAGATTTGCTTTAGAAGCCCATTTGGTGCACCAAAGCCCAACAGGAAGTGTTGCTGTCATTGGTGTTCTCTACAAAATTGGACGACCTGACTATTTCTTGTCCAAGATGAAAGAGTATTTGGAAGAAGTTTCTGATACAAAAGAAGATGAACCAATTGATTTGGCTGATCCGTCACAATTAGAGATGAGAAGCAGTTTATATTATCGATATATTGGCTCTCTAACTGTTCCGCCGTGTTCTCAAAATGTGCTTTGGACCCTTGTCAGAAAGGTTAGGACCGTTTCACCTGAACAAGTGAAGTTACTTCGAGTGGCGGTTCATGATGACTCAGACACTAATGCAAGGCCTTTGCAACCGCTTAATGATCGAAAGATTCAACTCCGAGTCAAATCATGA

Protein sequence

MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS

Homology

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.3e-79
Identity = 146/261 (55.94%), Postives = 193/261 (73.95%), Query Frame = 0


Query: 6   FHALFCTFLCAFLLVSWRAMSQ-EVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQ 65
           F ALF  F    + +S  A S  EVED++EF+Y +  ++GP  WG+LK EW  C  G MQ
Sbjct: 12  FVALFSIF--TIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQ 71

Query: 66  SPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYFLKQF 125
           SPIDL++ERV IV H   L  +Y PSNATLKNRGHDIMLK+ +GAG +++NG  Y L+Q 
Sbjct: 72  SPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQL 131

Query: 126 HWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTK 185
           HWHSPSEHTINGRRFALE H+VH+     +AV+ VLYKIGR D F+  +++ LE +++ +
Sbjct: 132 HWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEME 191

Query: 186 EDEP-IDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAV 245
           E E  + + DP+++++ S  YYRY GSLT PPC+QNV W++VRKVRTV+ +QVKLLRVAV
Sbjct: 192 EAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAV 251

Query: 246 HDDSDTNARPLQPLNDRKIQL 265
           HDD+++NARP+QP N R + L
Sbjct: 252 HDDANSNARPVQPTNKRIVHL 270

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-70
Identity = 130/261 (49.81%), Postives = 182/261 (69.73%), Query Frame = 0


Query: 9   LFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPID 68
           +F     +  +VS      EVED+ +F+Y ++G++GP +WG LK EW  C  G MQSPID
Sbjct: 9   VFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPID 68

Query: 69  LLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW-GEGAGY-MEVNGTRYFLKQFHW 128
           L  +RV I  +   L+++Y PSNAT+KNRGHDIM+K+ G  AG  + +NGT Y L+Q HW
Sbjct: 69  LTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHW 128

Query: 129 HSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTKE- 188
           HSPSEHT+NG+RF LE H+VHQS  G  AV+   YK+G+PDYFL  ++ YL+ ++DT E 
Sbjct: 129 HSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHES 188

Query: 189 DEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHD 248
            E +++  P      S  YYR+IGSLT PPCS+NV+WT+ +++RTV+ +Q+ +LRV VHD
Sbjct: 189 QEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHD 248

Query: 249 DSDTNARPLQPLNDRKIQLRV 267
            S++NARPLQ  N+R + L +
Sbjct: 249 QSNSNARPLQRKNERPVALYI 269

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 266.5 bits (680), Expect = 3.2e-70
Identity = 130/241 (53.94%), Postives = 171/241 (70.95%), Query Frame = 0


Query: 28  EVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTNLKTEY 87
           EVED+ EF Y  +G +GP+ WG L  EW  C  G MQSPIDL  + V +   F  L+++Y
Sbjct: 133 EVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLLRSQY 192

Query: 88  KPSNATLKNRGHDIMLKW---GEGAGYMEVNGTRYFLKQFHWHSPSEHTINGRRFALEAH 147
            PSN T+KNRGHDIMLK+    +G G + + GTRY L+Q HWHSPSEHTING+RFALE H
Sbjct: 193 LPSNTTIKNRGHDIMLKFKGGNKGIG-VTIRGTRYQLQQLHWHSPSEHTINGKRFALEEH 252

Query: 148 LVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDT-KEDEPIDLADPSQLEMRSSL 207
           LVH+S     AV+  LY +G  D FL  +++ L++++DT   +E + + DP +L   S  
Sbjct: 253 LVHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFESKH 312

Query: 208 YYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQ 265
           YYRY GSLT PPCS+NV+W++ +++RTVS +QVKLLRVAVHD SD+NARPLQ +N RK+ 
Sbjct: 313 YYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKRKVY 372

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 8.6e-68
Identity = 130/256 (50.78%), Postives = 183/256 (71.48%), Query Frame = 0


Query: 9   LFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPID 68
           LF +FL  FL   + A S EV+D+ EF Y+E+ + GP++WG+++ +W  C +G +QSPID
Sbjct: 10  LFISFL--FLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSPID 69

Query: 69  LLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYFLKQFHWHS 128
           +   R  +V +   L+ +YKPSNATL NRGHDIML+  +G GY+++N T+Y LKQ HWH+
Sbjct: 70  IFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLRLDDG-GYLKINETQYQLKQLHWHT 129

Query: 129 PSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGR-PDYFLSKMKEYLEEVSDTKE-D 188
           PSEHTING RF LEAHLVH+S  G   VIG++Y+IG  PD FLS ++  L+  ++ K  +
Sbjct: 130 PSEHTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPANKKGIE 189

Query: 189 EPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHDD 248
             I + DP+Q+++    Y+RYIGSLT PPC++ V+W + RKV+TV+  Q+KLL+ AVHD 
Sbjct: 190 RGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEAVHDG 249

Query: 249 SDTNARPLQPLNDRKI 263
            +TNARP QP N+R I
Sbjct: 250 FETNARPTQPENERYI 261

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 3.6e-66
Identity = 125/236 (52.97%), Postives = 163/236 (69.07%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFL----LVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWH 60
           M+K+S        L +F+     +S     +EVED+ EF Y    + GP+ WG L+ EW 
Sbjct: 1   MDKISIRCFIFLVLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWK 60

Query: 61  SCKAGLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWG-EGAGYMEVN 120
            C  G MQSPIDL+++RVR+V H   L   YKP NATLKNRGHD+MLK+G EG+G + VN
Sbjct: 61  MCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVN 120

Query: 121 GTRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKE 180
           GT Y L Q HWHSPSEHT+NGRRFALE H+VH++  GS+AV+ VLYKIGRPD FL  ++ 
Sbjct: 121 GTEYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLEN 180

Query: 181 YLEEVSDTKEDEP-IDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRT 231
            L  ++D  E E  +D+ DP  +++ S  +YRYIGSLT PPC+QNV+WT+V+KV T
Sbjct: 181 KLSAITDQNEAEKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

[Close]

BLAST of Cp4.1LG20g07300 vs. NCBI nr
Match: XP_023519286.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo] >XP_023519287.1 alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555 bits (1430), Expect = 1.24e-199
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
           SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR
Sbjct: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. NCBI nr
Match: XP_022970332.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 540 bits (1391), Expect = 1.10e-193
Identity = 258/268 (96.27%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           M+KLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKP NATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKM+EYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
           SDT+ED+ IDLADPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRK+RTVSPEQVKLLR
Sbjct: 181 SDTREDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLR+KS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. NCBI nr
Match: XP_022970333.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 538 bits (1386), Expect = 6.34e-193
Identity = 258/268 (96.27%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           M+KLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKP NATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKM+E+LEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEHLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
           SDT+ED+ IDLADPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRK+RTVSPEQVKLLR
Sbjct: 181 SDTQEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. NCBI nr
Match: KAG7020784.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538 bits (1385), Expect = 9.01e-193
Identity = 259/268 (96.64%), Postives = 263/268 (98.13%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFLLVSW AMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQS TGSVAVIGVLYKIGRPDYFLSKMKEYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
            DTKED+ IDLADPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRKV+TVSPEQVKLLR
Sbjct: 181 CDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLR+KS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. NCBI nr
Match: KAG6583573.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 537 bits (1384), Expect = 1.28e-192
Identity = 259/268 (96.64%), Postives = 263/268 (98.13%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSW AMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQS TGSVAVIGVLYKIGRPDYFLSKMKEYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
            DTKED+ IDLADPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRKV+TVSPEQVKLLR
Sbjct: 181 CDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy TrEMBL
Match: A0A6J1HYT6 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469328 PE=3 SV=1)

HSP 1 Score: 540 bits (1391), Expect = 5.31e-194
Identity = 258/268 (96.27%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           M+KLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKP NATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKM+EYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
           SDT+ED+ IDLADPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRK+RTVSPEQVKLLR
Sbjct: 181 SDTREDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLR+KS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy TrEMBL
Match: A0A6J1I3K8 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469329 PE=3 SV=1)

HSP 1 Score: 538 bits (1386), Expect = 3.07e-193
Identity = 258/268 (96.27%), Postives = 265/268 (98.88%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           M+KLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKP NATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKM+E+LEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEHLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
           SDT+ED+ IDLADPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRK+RTVSPEQVKLLR
Sbjct: 181 SDTQEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy TrEMBL
Match: A0A6J1HKB2 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464883 PE=3 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 1.20e-190
Identity = 255/268 (95.15%), Postives = 262/268 (97.76%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSW AMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
            DTKED+ IDL  PSQLEM SSLYY+YIGSLTVPPCSQNVLWT+VRKV+TVSPEQVKLLR
Sbjct: 181 CDTKEDKAIDLGHPSQLEMLSSLYYQYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPL+DR+IQLRVKS
Sbjct: 241 VAVHDDSDTNARPLQPLHDRRIQLRVKS 268

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy TrEMBL
Match: A0A6J1HME8 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464898 PE=3 SV=1)

HSP 1 Score: 469 bits (1207), Expect = 1.78e-166
Identity = 225/235 (95.74%), Postives = 228/235 (97.02%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSW AMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           LKQFHWHSPSEHTINGRRFALEAHLVHQS TGSVAVIGVLYKIGRPDYFLSKMKEYLEEV
Sbjct: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQ 235
            DTKED+ IDL DPSQLEM SSLYYRYIGSLTVPPCSQNVLWT+VRKVRTVS EQ
Sbjct: 181 CDTKEDKAIDLGDPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVRTVSLEQ 235

[Close]

BLAST of Cp4.1LG20g07300 vs. ExPASy TrEMBL
Match: A0A6J1KFK2 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111493412 PE=3 SV=1)

HSP 1 Score: 460 bits (1183), Expect = 2.68e-162
Identity = 215/268 (80.22%), Postives = 243/268 (90.67%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKL FH +F  FLCAFLLVSW  M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MEKLPFHVIFGAFLCAFLLVSWPVMCQEVEDQKEFDYDAEGDRGPTHWGDLKREWHGCKT 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYF 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKPSNATLKNRGHDIMLKW  GAG + VNGTRYF
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKWSGGAGSINVNGTRYF 120

Query: 121 LKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEV 180
           L+Q HWHSPSEHTINGRRFALEAHLVHQS TG VAVIG+LY IGRPD+FLSK++EYLE++
Sbjct: 121 LRQCHWHSPSEHTINGRRFALEAHLVHQSQTGKVAVIGLLYNIGRPDHFLSKLEEYLEDI 180

Query: 181 SDTKEDEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLR 240
           S+++E + +++ DPS L+M+SSLYYRYIGSLTVPPCSQNVLWT+VR++RTV+PEQVKLLR
Sbjct: 181 SNSREYDELNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQVKLLR 240

Query: 241 VAVHDDSDTNARPLQPLNDRKIQLRVKS 268
           VAVHDDSDTNARPLQPLNDRKIQLR+K+
Sbjct: 241 VAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of Cp4.1LG20g07300 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 297.7 bits (761), Expect = 9.1e-81
Identity = 146/261 (55.94%), Postives = 193/261 (73.95%), Query Frame = 0


Query: 6   FHALFCTFLCAFLLVSWRAMSQ-EVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQ 65
           F ALF  F    + +S  A S  EVED++EF+Y +  ++GP  WG+LK EW  C  G MQ
Sbjct: 12  FVALFSIF--TIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQ 71

Query: 66  SPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYFLKQF 125
           SPIDL++ERV IV H   L  +Y PSNATLKNRGHDIMLK+ +GAG +++NG  Y L+Q 
Sbjct: 72  SPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQL 131

Query: 126 HWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTK 185
           HWHSPSEHTINGRRFALE H+VH+     +AV+ VLYKIGR D F+  +++ LE +++ +
Sbjct: 132 HWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEME 191

Query: 186 EDEP-IDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAV 245
           E E  + + DP+++++ S  YYRY GSLT PPC+QNV W++VRKVRTV+ +QVKLLRVAV
Sbjct: 192 EAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAV 251

Query: 246 HDDSDTNARPLQPLNDRKIQL 265
           HDD+++NARP+QP N R + L
Sbjct: 252 HDDANSNARPVQPTNKRIVHL 270

[Close]

BLAST of Cp4.1LG20g07300 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 268.1 bits (684), Expect = 7.7e-72
Identity = 130/261 (49.81%), Postives = 182/261 (69.73%), Query Frame = 0


Query: 9   LFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPID 68
           +F     +  +VS      EVED+ +F+Y ++G++GP +WG LK EW  C  G MQSPID
Sbjct: 9   VFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPID 68

Query: 69  LLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW-GEGAGY-MEVNGTRYFLKQFHW 128
           L  +RV I  +   L+++Y PSNAT+KNRGHDIM+K+ G  AG  + +NGT Y L+Q HW
Sbjct: 69  LTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIHW 128

Query: 129 HSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTKE- 188
           HSPSEHT+NG+RF LE H+VHQS  G  AV+   YK+G+PDYFL  ++ YL+ ++DT E 
Sbjct: 129 HSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHES 188

Query: 189 DEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHD 248
            E +++  P      S  YYR+IGSLT PPCS+NV+WT+ +++RTV+ +Q+ +LRV VHD
Sbjct: 189 QEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHD 248

Query: 249 DSDTNARPLQPLNDRKIQLRV 267
            S++NARPLQ  N+R + L +
Sbjct: 249 QSNSNARPLQRKNERPVALYI 269

[Close]

BLAST of Cp4.1LG20g07300 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 251.5 bits (641), Expect = 7.5e-67
Identity = 119/208 (57.21%), Postives = 152/208 (73.08%), Query Frame = 0


Query: 25  MSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTNLK 84
           M + VED+ EF Y    + GP+ WG L+ EW  C  G MQSPIDL+++RVR+V H   L 
Sbjct: 1   MDEYVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLT 60

Query: 85  TEYKPSNATLKNRGHDIMLKWG-EGAGYMEVNGTRYFLKQFHWHSPSEHTINGRRFALEA 144
             YKP NATLKNRGHD+MLK+G EG+G + VNGT Y L Q HWHSPSEHT+NGRRFALE 
Sbjct: 61  RHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALEL 120

Query: 145 HLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTKEDEP-IDLADPSQLEMRSS 204
           H+VH++  GS+AV+ VLYKIGRPD FL  ++  L  ++D  E E  +D+ DP  +++ S 
Sbjct: 121 HMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSR 180

Query: 205 LYYRYIGSLTVPPCSQNVLWTLVRKVRT 231
            +YRYIGSLT PPC+QNV+WT+V+KV T
Sbjct: 181 KFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

[Close]

BLAST of Cp4.1LG20g07300 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-59
Identity = 112/257 (43.58%), Postives = 165/257 (64.20%), Query Frame = 0


Query: 9   LFCTFLCAFLLVSWRAMS---QEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQS 68
           +F   +C F+ +S+  +S    EV+D+  F Y ++ ++GP  WG +   W  C  G  QS
Sbjct: 8   IFFMAMC-FIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQS 67

Query: 69  PIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYFLKQFH 128
           PIDL +ERV ++ H      +YKP+ A + NRGHDIM+ W   AG M +  T + L Q H
Sbjct: 68  PIDLTNERVSLI-HDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCH 127

Query: 129 WHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTKE 188
           WHSPSEHT+NG R+ LE H+VH S  G  AVIGVLYK+G P+ FL+K+   ++ V +  +
Sbjct: 128 WHSPSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGN--K 187

Query: 189 DEPIDLADPSQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHD 248
           +  + + DP ++  ++  +YRYIGSLTVPPC++ V+WT+V++V T+S EQ+  LR AV D
Sbjct: 188 EINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDD 247

Query: 249 DSDTNARPLQPLNDRKI 263
             +TN+RP+Q    R +
Sbjct: 248 GFETNSRPVQDSKGRSV 260

[Close]

BLAST of Cp4.1LG20g07300 vs. TAIR 10
Match: AT5G04180.1 (alpha carbonic anhydrase 3 )

HSP 1 Score: 209.9 bits (533), Expect = 2.5e-54
Identity = 109/254 (42.91%), Postives = 158/254 (62.20%), Query Frame = 0


Query: 17  FLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRI 76
           FL +S  +++ E E   EF Y       PS W  +K EW  C  G  QSPI+L  +  RI
Sbjct: 10  FLALSSSSLADETE--TEFHYKPGEIADPSKWSSIKAEWKICGTGKRQSPINLTPKIARI 69

Query: 77  VPHFTN-LKTEYKPSNATLKNRGHDIMLKWGEGAGYMEVNGTRYFLKQFHWHSPSEHTIN 136
           V + T  L+T YKP  A LKNRG D+ +KW + AG + +N T Y L Q HWH+PSEH ++
Sbjct: 70  VHNSTEILQTYYKPVEAILKNRGFDMKVKWEDDAGKIVINDTDYKLVQSHWHAPSEHFLD 129

Query: 137 GRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEYLEEVSDTKEDE-PIDLADP 196
           G+R A+E H+VH+S  G +AVIGVL++ G P+ F+S++ + + +++D ++ E  I   DP
Sbjct: 130 GQRLAMELHMVHKSVEGHLAVIGVLFREGEPNAFISRIMDKIHKIADVQDGEVSIGKIDP 189

Query: 197 SQLEMRSSLYYRYIGSLTVPPCSQNVLWTLVRKVRTVSPEQVKLLRVAVHDDSDTNARPL 256
            +     + +Y Y GSLT PPC+++V+WT++ KV TVS EQ+ +L  A     + NARP 
Sbjct: 190 REFGWDLTKFYEYRGSLTTPPCTEDVMWTIINKVGTVSREQIDVLTDARRGGYEKNARPA 249

Query: 257 QPLNDRKIQLRVKS 269
           QPLN R + L  +S
Sbjct: 250 QPLNGRLVYLNEQS 261

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g07300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L817	1.3e-79	55.94	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
F4HUC4	1.1e-70	49.81	Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1	[more]
Q9FM99	3.2e-70	53.94	Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3	[more]
Q84UV8	8.6e-68	50.78	Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS...	[more]
F4IHR4	3.6e-66	52.97	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2	[more]

BLAST of Cp4.1LG20g07300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519286.1	1.24e-199	100.00	alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo] >XP_023519287.1 alp...	[more]
XP_022970332.1	1.10e-193	96.27	alpha carbonic anhydrase 7-like [Cucurbita maxima]	[more]
XP_022970333.1	6.34e-193	96.27	alpha carbonic anhydrase 7-like [Cucurbita maxima]	[more]
KAG7020784.1	9.01e-193	96.64	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6583573.1	1.28e-192	96.64	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cp4.1LG20g07300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HYT6	5.31e-194	96.27	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469328 PE=3 SV=1	[more]
A0A6J1I3K8	3.07e-193	96.27	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469329 PE=3 SV=1	[more]
A0A6J1HKB2	1.20e-190	95.15	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464883 PE=3 SV=1	[more]
A0A6J1HME8	1.78e-166	95.74	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464898 PE=3 SV=1	[more]
A0A6J1KFK2	2.68e-162	80.22	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111493412 PE=3 SV=1	[more]

BLAST of Cp4.1LG20g07300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08080.1	9.1e-81	55.94	alpha carbonic anhydrase 7	[more]
AT1G08065.1	7.7e-72	49.81	alpha carbonic anhydrase 5	[more]
AT2G28210.1	7.5e-67	57.21	alpha carbonic anhydrase 2	[more]
AT4G20990.1	1.5e-59	43.58	alpha carbonic anhydrase 4	[more]
AT5G04180.1	2.5e-54	42.91	alpha carbonic anhydrase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 37..266 e-value: 1.5E-72 score: 257.0
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 36..263 e-value: 8.2E-43 score: 146.8
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 33..266 score: 46.499462
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 31..267 e-value: 5.3E-76 score: 257.3
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 35..263
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 9..264
None	No IPR available	PANTHER	PTHR18952:SF240	ALPHA CARBONIC ANHYDRASE 7-LIKE	coord: 9..264
IPR018338	Carbonic anhydrase, alpha-class, conserved site	PROSITE	PS00162	ALPHA_CA_1	coord: 130..146
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 44..264 e-value: 6.93582E-98 score: 283.779

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g07300.1	Cp4.1LG20g07300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006730	one-carbon metabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding