Cp4.1LG20g05970 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g05970 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 3.2e-53
Identity = 159/529 (30.06%), Postives = 276/529 (52.17%), Query Frame = 0

Query: 377  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 436
            N L   IS++C+S+ L ECE A+  +++   D   +  I   LS P  + G++E+L AS 
Sbjct: 658  NSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 717

Query: 437  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 496
            + E+L   I IL+EL    +++   + + D        LLK+     +A++L+Y  +P  
Sbjct: 718  NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LAEAALLIYQLRPVF 777

Query: 497  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 556
             Q+ + E +P ++ V++        F +   P +AA  +L+Q L G DE     N   +I
Sbjct: 778  AQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVI 837

Query: 557  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 616
            +  G+  L++ LE   +E R++ VS++ CC++A+ +C+N +A  +  + +LEL  H  N 
Sbjct: 838  SANGIPTLVKYLE--RMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLEL-FHSGND 897

Query: 617  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 676
            +     +  L EL+ L+RRT   +LL  +K+     S M+   V L  A  E Q  VA++
Sbjct: 898  SVRGTCVEFLSELVQLNRRTSCNQLLHTIKD-EGAFSTMHTFLVYLQMAPMEHQLAVASL 957

Query: 677  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 736
            LLQLDL+ +P    SI+REEA+ T+I AL  Q     T      ALL L G  S +G+  
Sbjct: 958  LLQLDLLAEPRK-MSIYREEAVETLIEAL-WQKDFSNTQMKALDALLFLIGHISSSGKSY 1017

Query: 737  PEIWLLQQAGFET--NSGDSSYSSRHVYDDLVQSYEEEEDLVN-WQLKASTVFFNHGHKS 796
             E WLL+ AGF+   N+           +DL+++ E+E++ +N WQ + ++V  NH + S
Sbjct: 1018 TEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIASVLCNHENGS 1077

Query: 797  LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 856
            +  AL   + +   ++AK+ LV  +W++R L+ + D  +  +A   L+  +IK L+  K 
Sbjct: 1078 IFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLLEEVIKVLHSSKS 1137

Query: 857  VEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEELI 903
            +ED +L + +L   I       +  ++ +     L  L   +  A +++
Sbjct: 1138 LEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1178

HSP 2 Score: 67.4 bits (163), Expect = 9.7e-10
Identity = 52/197 (26.40%), Postives = 91/197 (46.19%), Query Frame = 0

Query: 137 ESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTVISSLNSSKKSKNCAQS 196
           +  V +N + G+++++  I   +   +  R+  +   L     V + LN  +K      S
Sbjct: 64  DQAVLANLDWGIEALEEAINTYNMETKLARLDYAEKML----QVCAMLNPKQKIAGVPNS 123

Query: 197 HLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLPELWEHFFLPHLLHLKV 256
           +LSA AHL L+ ++K+  N +    H L++F   PFF R    PELW+  FLPH+  +  
Sbjct: 124 YLSAWAHLNLSYLWKLRNNVQNCISHALEMFIVDPFFTRIDFAPELWKSLFLPHMSSIVG 183

Query: 257 WYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDGAKAPHVPLVPLPSQSI 316
           WY++E        + + + ++  D  +   L F   +EQ  N+       P      Q +
Sbjct: 184 WYSEE--------RHRLMMEVIPDSAD---LSFTADFEQFFNESLVLTMRPHQLEKLQKL 243

Query: 317 KRASRKSLDSSNNKNLY 334
           ++   +SLD   N  LY
Sbjct: 244 EQLYGESLD--ENTKLY 243

BLAST of Cp4.1LG20g05970 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 2.7e-52
Identity = 156/532 (29.32%), Postives = 276/532 (51.88%), Query Frame = 0

Query: 377  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 436
            N L   IS++C+S+ L ECE A+  +++   D   +  I   LS P  + G++E+L AS 
Sbjct: 658  NSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 717

Query: 437  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 496
            + E+L   I IL+EL    +++   + + D        LLK+     +A++L+Y  +P  
Sbjct: 718  NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LAEAALLIYQLRPVF 777

Query: 497  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 556
             Q+ + E +P ++ V++        F +   P +AA  +L+Q L G DE     N   +I
Sbjct: 778  AQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVI 837

Query: 557  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 616
            +  G+  L++ LE   +E R++ VS++ CC++A+ +C+N +A  +  + +LEL  H  N 
Sbjct: 838  SANGIPTLVKYLE--RMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLEL-FHSGND 897

Query: 617  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 676
            +     +  L EL+ L+RRT   ++L  +K+     S M+   V L  A  E Q  VA++
Sbjct: 898  SVRGTCVEFLSELVQLNRRTSCNQILHTIKD-EGAFSTMHTFLVYLQMAPMEHQLAVASL 957

Query: 677  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 736
            LLQLDL+ +P    SI+REEA+ T+I AL  Q     T      ALL L G  S +G+  
Sbjct: 958  LLQLDLLAEPRK-MSIYREEAVETLIEAL-WQKDFSNTQMKALDALLFLIGHISSSGKSY 1017

Query: 737  PEIWLLQQAGFET--NSGDSSYSSRHVYDDLVQSYEEEEDLVN-WQLKASTVFFNHGHKS 796
             E WLL+ AGF+   N+           +DL+++ E+E++ +N WQ + ++V  NH + S
Sbjct: 1018 TEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIASVLCNHENGS 1077

Query: 797  LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 856
            +  AL   + +   ++AK+ LV  +W++  L+ + D  +  +A   L+  +I  L   K+
Sbjct: 1078 IFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLLEEVINVLQSSKN 1137

Query: 857  VEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEELISIV 906
            +E+++LA+  L   I          ++ +     L  L   +  A +++ ++
Sbjct: 1138 LEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDIMKVI 1181

HSP 2 Score: 67.4 bits (163), Expect = 9.7e-10
Identity = 52/197 (26.40%), Postives = 91/197 (46.19%), Query Frame = 0

Query: 137 ESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTVISSLNSSKKSKNCAQS 196
           +  V +N + G+++++  I   +   +  R+  +   L     V + LN  +K      S
Sbjct: 64  DQAVLANLDWGIEALEEAINTYNMETKLARLDYAEKML----QVCAMLNPKQKIAGVPNS 123

Query: 197 HLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLPELWEHFFLPHLLHLKV 256
           +LSA AHL L+ ++K+  N +    H L++F   PFF R    PELW+  FLPH+  +  
Sbjct: 124 YLSAWAHLNLSYLWKLRNNVQNCISHALEMFIVDPFFTRIDFAPELWKSLFLPHMSSIVG 183

Query: 257 WYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDGAKAPHVPLVPLPSQSI 316
           WY++E        + + + ++  D  +   L F   +EQ  N+       P      Q +
Sbjct: 184 WYSEE--------RHRLMMEVIPDSAD---LSFTADFEQFFNESLVLTMRPHQLEKLQKL 243

Query: 317 KRASRKSLDSSNNKNLY 334
           ++   +SLD   N  LY
Sbjct: 244 EQLYGESLD--ENTKLY 243

BLAST of Cp4.1LG20g05970 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.6e-49
Identity = 152/529 (28.73%), Postives = 273/529 (51.61%), Query Frame = 0

Query: 377  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 436
            N L   I+++C+S+ L +CE A+  +++ W D   D  I   LS P  V G++E+L AS 
Sbjct: 661  NSLTPYITSLCTSENLQDCEQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASL 720

Query: 437  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 496
            + E+L   I IL+EL    E +   + + D        LLK+     +A++L+Y  +P  
Sbjct: 721  NREVLRRSIYILSELIFSDERVGETLNSVDSDFDCLAMLLKNG--LAEAALLIYQLRPVF 780

Query: 497  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 556
             Q+   E +P +++V++        F +   P  AA  +L+Q+L G DE     N   +I
Sbjct: 781  AQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVI 840

Query: 557  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 616
            +  G+  +++ L+  + E R+  +S++ CC++A+ +C++ +A  +  + +LEL  H  N 
Sbjct: 841  SANGIPAIVKYLD--KTEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLEL-FHAGND 900

Query: 617  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 676
            +     +  L EL+ L+RRT   + L  +K+     S M+   V L  A  E Q  VA++
Sbjct: 901  SVRGICVEFLSELVRLNRRTSSNQTLQIIKD-EGAFSTMHTFLVYLQMAPMEHQIAVASL 960

Query: 677  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 736
            LLQLDL+ +P    SI+REEA+ T+I AL  Q            ALL L G  + +G+  
Sbjct: 961  LLQLDLLAEPRK-MSIYREEAVETLIEAL-WQKDFSNNQMKALDALLFLIGHVTSSGKSY 1020

Query: 737  PEIWLLQQAGFET--NSGDSSYSSRHVYDDLVQSYEEEED-LVNWQLKASTVFFNHGHKS 796
             E  LL+ AGF+   N    +    H  +D +++ E+E++ + +WQ + ++V  NH + S
Sbjct: 1021 TEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVASVLCNHENGS 1080

Query: 797  LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 856
            +  AL   + +   ++AK+ LV  +W++  LF + D  +  +A   L+ +L+  L   K+
Sbjct: 1081 IFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKN 1140

Query: 857  VEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEELI 903
            +E+++LAS  L + I       +  ++ +     L  L   +  A +++
Sbjct: 1141 LEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1181

HSP 2 Score: 70.9 bits (172), Expect = 8.8e-11
Identity = 39/125 (31.20%), Postives = 68/125 (54.40%), Query Frame = 0

Query: 137 ESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTVISSLNSSKKSKNCAQS 196
           +  V +N + G+++++  I   +   +  R+  +   L     V + LN  +K+     S
Sbjct: 64  DQAVLANLDWGIEALEEAINTYNMETKLARLDYAEKML----QVCAMLNPKQKTAGVPNS 123

Query: 197 HLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLPELWEHFFLPHLLHLKV 256
           +LSA AHL L+ ++K+  N K    H L++F   PFF+R    PELW++ FLPH+  +  
Sbjct: 124 YLSAWAHLNLSYLWKLRNNIKSCIYHSLEMFIVDPFFSRIDFAPELWKNLFLPHMSSIVG 183

Query: 257 WYNQE 262
           WY++E
Sbjct: 184 WYSEE 184

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: XP_023520024.1 (putative E3 ubiquitin-protein ligase LIN-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1791 bits (4640), Expect = 0.0
Identity = 928/928 (100.00%), Postives = 928/928 (100.00%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE
Sbjct: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP
Sbjct: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS
Sbjct: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNSYTQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: XP_023520025.1 (putative E3 ubiquitin-protein ligase LIN-2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1785 bits (4623), Expect = 0.0
Identity = 927/928 (99.89%), Postives = 927/928 (99.89%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE
Sbjct: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP
Sbjct: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS
Sbjct: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSR TRITKLLDELKERSNGSSIMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSR-TRITKLLDELKERSNGSSIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNSYTQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 927

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: XP_022927577.1 (putative E3 ubiquitin-protein ligase LIN-2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1737 bits (4499), Expect = 0.0
Identity = 895/928 (96.44%), Postives = 914/928 (98.49%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           M+SLKELLTQEG+EGSDFSRAQKHS SKARSRAAP+DSITLPIYICHDKK TDSSKKKVE
Sbjct: 1   MSSLKELLTQEGLEGSDFSRAQKHSGSKARSRAAPNDSITLPIYICHDKKTTDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDG SLYSSNRLGSISETLLCKSMEA AIDEI+IRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGSSLYSSNRLGSISETLLCKSMEAPAIDEIAIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+KKSKNCAQSHLSACAHLYLA VYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSAKKSKNCAQSHLSACAHLYLATVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPS SI+RASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSHSIRRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNL+TNHLLSNELCKAI+TI SSDILSECEIAIRVV+KAWLDVDGDTTIEVALS
Sbjct: 361 KKSDYFRNLDTNHLLSNELCKAIATISSSDILSECEIAIRVVTKAWLDVDGDTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPPF+EGM+EVLLASNDDEILELVIS+LAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPFIEGMMEVLLASNDDEILELVISVLAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKN +SMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNAISMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKER NGSSIMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERLNGSSIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGFE NSGDSSYSSRHVYD+LVQ YEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFEANSGDSSYSSRHVYDELVQLYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALST+MTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTAMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASY+LLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTA+E
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYSLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAKE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSDTQRSTRM 928

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: XP_022927578.1 (putative E3 ubiquitin-protein ligase LIN-2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1731 bits (4482), Expect = 0.0
Identity = 894/928 (96.34%), Postives = 913/928 (98.38%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           M+SLKELLTQEG+EGSDFSRAQKHS SKARSRAAP+DSITLPIYICHDKK TDSSKKKVE
Sbjct: 1   MSSLKELLTQEGLEGSDFSRAQKHSGSKARSRAAPNDSITLPIYICHDKKTTDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDG SLYSSNRLGSISETLLCKSMEA AIDEI+IRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGSSLYSSNRLGSISETLLCKSMEAPAIDEIAIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+KKSKNCAQSHLSACAHLYLA VYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSAKKSKNCAQSHLSACAHLYLATVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPS SI+RASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSHSIRRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNL+TNHLLSNELCKAI+TI SSDILSECEIAIRVV+KAWLDVDGDTTIEVALS
Sbjct: 361 KKSDYFRNLDTNHLLSNELCKAIATISSSDILSECEIAIRVVTKAWLDVDGDTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPPF+EGM+EVLLASNDDEILELVIS+LAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPFIEGMMEVLLASNDDEILELVISVLAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKN +SMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNAISMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSR TRITKLLDELKER NGSSIMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSR-TRITKLLDELKERLNGSSIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGFE NSGDSSYSSRHVYD+LVQ YEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFEANSGDSSYSSRHVYDELVQLYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALST+MTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTAMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASY+LLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTA+E
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYSLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAKE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSDTQRSTRM 927

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: KAG7019436.1 (putative E3 ubiquitin-protein ligase LIN-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1720 bits (4454), Expect = 0.0
Identity = 891/928 (96.01%), Postives = 905/928 (97.52%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           MASLKELLTQEG+EGSDFSRA+KHSSSKARSRAAP+DSITLPIYICHDKK TDSSKKKVE
Sbjct: 1   MASLKELLTQEGLEGSDFSRAKKHSSSKARSRAAPNDSITLPIYICHDKKTTDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDG SLYSSNRLGSISETLLCKSMEASAIDEI+IRSVVSILSGYVGRY KDDKFRE
Sbjct: 61  KSIMRDGSSLYSSNRLGSISETLLCKSMEASAIDEIAIRSVVSILSGYVGRYLKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+ KSKNC +SHLSACAHLYLAIVYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSANKSKNCVKSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAP VPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPDVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNL+TNHLLSNELCKAI+TI SSDILSECEIAIRVV+KAWLDVDGDTTIEVAL 
Sbjct: 361 KKSDYFRNLDTNHLLSNELCKAIATISSSDILSECEIAIRVVTKAWLDVDGDTTIEVALL 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPP VEGMLEVLLASNDDEILELVIS+LAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPVVEGMLEVLLASNDDEILELVISVLAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMI VEWLPLVLRVLEFGGSLQMLFSVRC PYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMILVEWLPLVLRVLEFGGSLQMLFSVRCVPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKN +SMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNAISMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGS IMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSRIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRA PEEQPLVATILLQLDL+DDPLNCCSIFREEAIVTIITALNSQISREKT ENLAR
Sbjct: 661 DLHRAHPEEQPLVATILLQLDLVDDPLNCCSIFREEAIVTIITALNSQISREKTLENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGF  NSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFVANSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSM PSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMTPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEAR HLENLSLVTWTAEE
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARVHLENLSLVTWTAEE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQ NS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQNNSDTQRSTRM 928

BLAST of Cp4.1LG20g05970 vs. ExPASy TrEMBL
Match: A0A6J1EHK0 (putative E3 ubiquitin-protein ligase LIN-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434366 PE=4 SV=1)

HSP 1 Score: 1737 bits (4499), Expect = 0.0
Identity = 895/928 (96.44%), Postives = 914/928 (98.49%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           M+SLKELLTQEG+EGSDFSRAQKHS SKARSRAAP+DSITLPIYICHDKK TDSSKKKVE
Sbjct: 1   MSSLKELLTQEGLEGSDFSRAQKHSGSKARSRAAPNDSITLPIYICHDKKTTDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDG SLYSSNRLGSISETLLCKSMEA AIDEI+IRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGSSLYSSNRLGSISETLLCKSMEAPAIDEIAIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+KKSKNCAQSHLSACAHLYLA VYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSAKKSKNCAQSHLSACAHLYLATVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPS SI+RASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSHSIRRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNL+TNHLLSNELCKAI+TI SSDILSECEIAIRVV+KAWLDVDGDTTIEVALS
Sbjct: 361 KKSDYFRNLDTNHLLSNELCKAIATISSSDILSECEIAIRVVTKAWLDVDGDTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPPF+EGM+EVLLASNDDEILELVIS+LAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPFIEGMMEVLLASNDDEILELVISVLAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKN +SMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNAISMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKER NGSSIMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERLNGSSIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGFE NSGDSSYSSRHVYD+LVQ YEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFEANSGDSSYSSRHVYDELVQLYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALST+MTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTAMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASY+LLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTA+E
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYSLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAKE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSDTQRSTRM 928

BLAST of Cp4.1LG20g05970 vs. ExPASy TrEMBL
Match: A0A6J1ELD9 (putative E3 ubiquitin-protein ligase LIN-2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434366 PE=4 SV=1)

HSP 1 Score: 1731 bits (4482), Expect = 0.0
Identity = 894/928 (96.34%), Postives = 913/928 (98.38%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           M+SLKELLTQEG+EGSDFSRAQKHS SKARSRAAP+DSITLPIYICHDKK TDSSKKKVE
Sbjct: 1   MSSLKELLTQEGLEGSDFSRAQKHSGSKARSRAAPNDSITLPIYICHDKKTTDSSKKKVE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDG SLYSSNRLGSISETLLCKSMEA AIDEI+IRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGSSLYSSNRLGSISETLLCKSMEAPAIDEIAIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+KKSKNCAQSHLSACAHLYLA VYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSAKKSKNCAQSHLSACAHLYLATVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPS SI+RASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSHSIRRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYFRNL+TNHLLSNELCKAI+TI SSDILSECEIAIRVV+KAWLDVDGDTTIEVALS
Sbjct: 361 KKSDYFRNLDTNHLLSNELCKAIATISSSDILSECEIAIRVVTKAWLDVDGDTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPPF+EGM+EVLLASNDDEILELVIS+LAELAARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPFIEGMMEVLLASNDDEILELVISVLAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKN +SMISCCIRADGTCRNYLAEN
Sbjct: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNAISMISCCIRADGTCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSR TRITKLLDELKER NGSSIMNILSV
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSR-TRITKLLDELKERLNGSSIMNILSV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGFE NSGDSSYSSRHVYD+LVQ YEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFEANSGDSSYSSRHVYDELVQLYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFNHGHKSLLSALST+MTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNHGHKSLLSALSTAMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASY+LLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTA+E
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYSLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAKE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSDTQRSTRM 927

BLAST of Cp4.1LG20g05970 vs. ExPASy TrEMBL
Match: A0A6J1KK68 (putative E3 ubiquitin-protein ligase LIN-2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495354 PE=4 SV=1)

HSP 1 Score: 1698 bits (4397), Expect = 0.0
Identity = 880/928 (94.83%), Postives = 903/928 (97.31%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           M SLKELLTQ G+EG DF+R  KHSSSKARSRAAP+DSITLPIYICHDKKITDSSKKK+E
Sbjct: 1   MVSLKELLTQ-GLEGRDFTRTHKHSSSKARSRAAPNDSITLPIYICHDKKITDSSKKKIE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDGFSLYSSNRLGSISETLLCKSMEA AIDEI+IRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEAPAIDEIAIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMK VDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKRVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+KKSKNC QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSAKKSKNCVQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYF+NLE NHLLSNELCKAI+TI SSDILSECEIAI VV+KAWLDV+G TTIEVALS
Sbjct: 361 KKSDYFQNLERNHLLSNELCKAIATISSSDILSECEIAIHVVTKAWLDVEGKTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPP VEGMLEVLLASNDDEILELVIS+LAE+AARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPVVEGMLEVLLASNDDEILELVISVLAEIAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVR AP+EAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRFAPHEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLEN RHLIALGGLSLLLR  E+GE+EERKN+VSMISCCIRADG+CRNYLAEN
Sbjct: 541 KGFDEDRNLENCRHLIALGGLSLLLRRFESGEVEERKNSVSMISCCIRADGSCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKER NGSSIMNIL V
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERWNGSSIMNILLV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLV+TILL+LDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVSTILLKLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGF+ NSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFKANSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFN GHKSLLSALSTSMTN IPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNQGHKSLLSALSTSMTNSIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASY+LLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYSLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSDTQRSTRM 927

BLAST of Cp4.1LG20g05970 vs. ExPASy TrEMBL
Match: A0A6J1KHQ5 (putative E3 ubiquitin-protein ligase LIN-2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495354 PE=4 SV=1)

HSP 1 Score: 1691 bits (4380), Expect = 0.0
Identity = 879/928 (94.72%), Postives = 902/928 (97.20%), Query Frame = 0

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           M SLKELLTQ G+EG DF+R  KHSSSKARSRAAP+DSITLPIYICHDKKITDSSKKK+E
Sbjct: 1   MVSLKELLTQ-GLEGRDFTRTHKHSSSKARSRAAPNDSITLPIYICHDKKITDSSKKKIE 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
           KSIMRDGFSLYSSNRLGSISETLLCKSMEA AIDEI+IRSVVSILSGYVGRYSKDDKFRE
Sbjct: 61  KSIMRDGFSLYSSNRLGSISETLLCKSMEAPAIDEIAIRSVVSILSGYVGRYSKDDKFRE 120

Query: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
           MVRKICSSCLLRNGGMESGVCSNFEMGMK VDRLIEERHGNERELRVKASR+SLGF N V
Sbjct: 121 MVRKICSSCLLRNGGMESGVCSNFEMGMKRVDRLIEERHGNERELRVKASRNSLGFLNMV 180

Query: 181 ISSLNSSKKSKNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLLP 240
           ISSLNS+KKSKNC QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVF+DSPFFARTHLLP
Sbjct: 181 ISSLNSAKKSKNCVQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFSDSPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300
           ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFMSNDRKIKALSKLYNDHMNRGTLKFALYYEQQLNDG 300

Query: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360
           AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV
Sbjct: 301 AKAPHVPLVPLPSQSIKRASRKSLDSSNNKNLYHAVFGPNLDQQLAEKIASVHDEHHETV 360

Query: 361 KKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALS 420
           KKSDYF+NLE NHLLSNELCKAI+TI SSDILSECEIAI VV+KAWLDV+G TTIEVALS
Sbjct: 361 KKSDYFQNLERNHLLSNELCKAIATISSSDILSECEIAIHVVTKAWLDVEGKTTIEVALS 420

Query: 421 TPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSS 480
           TPP VEGMLEVLLASNDDEILELVIS+LAE+AARSEAIRLMILNSDPQLQVFLKLLKSSS
Sbjct: 421 TPPVVEGMLEVLLASNDDEILELVISVLAEIAARSEAIRLMILNSDPQLQVFLKLLKSSS 480

Query: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLL 540
           LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVR AP+EAAFYLLDQLL
Sbjct: 481 LFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRFAPHEAAFYLLDQLL 540

Query: 541 KGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAEN 600
           KGFDEDRNLEN RHLIALGGLSLLLR  E+GE+EERKN+VSMISCCIRADG+CRNYLAEN
Sbjct: 541 KGFDEDRNLENCRHLIALGGLSLLLRRFESGEVEERKNSVSMISCCIRADGSCRNYLAEN 600

Query: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSV 660
           LNKAALLELVVHESNKNSDRCGLALLVELLCLSR TRITKLLDELKER NGSSIMNIL V
Sbjct: 601 LNKAALLELVVHESNKNSDRCGLALLVELLCLSR-TRITKLLDELKERWNGSSIMNILLV 660

Query: 661 DLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720
           DLHRARPEEQPLV+TILL+LDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR
Sbjct: 661 DLHRARPEEQPLVSTILLKLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLAR 720

Query: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780
           ALLILGGRFSCTGEPCPEIWLLQQAGF+ NSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL
Sbjct: 721 ALLILGGRFSCTGEPCPEIWLLQQAGFKANSGDSSYSSRHVYDDLVQSYEEEEDLVNWQL 780

Query: 781 KASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840
           KASTVFFN GHKSLLSALSTSMTN IPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL
Sbjct: 781 KASTVFFNQGHKSLLSALSTSMTNSIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSIL 840

Query: 841 VPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900
           VPSLIKYLNYDKDVEDRVLASY+LLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE
Sbjct: 841 VPSLIKYLNYDKDVEDRVLASYSLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEE 900

Query: 901 LISIVKSGSMHRYDTEQKNSYTQRSTRM 928
           LISIVKSGSMHRYDTEQKNS TQRSTRM
Sbjct: 901 LISIVKSGSMHRYDTEQKNSDTQRSTRM 926

BLAST of Cp4.1LG20g05970 vs. ExPASy TrEMBL
Match: A0A0A0LYR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G713660 PE=4 SV=1)

HSP 1 Score: 1328 bits (3437), Expect = 0.0
Identity = 732/1011 (72.40%), Postives = 821/1011 (81.21%), Query Frame = 0

Query: 1    MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
            MASL+ELLT+EG EGS++   +K S  K RSR APDDS+TLPIYICHDKK  DSSKKK +
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KSIMRDGFSLYSSNRLGSISETLLCKSMEASAIDEISIRSVVSILSGYVGRYSKDDKFRE 120
            K ++R+G S+YSS R+GS+SETL CKSME  AIDEI+IR+VVSILSGYVGRYSKD+ FRE
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  MVRKICSSCLLRNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLGFFNTV 180
            +VRK C+ CL+R G MESG+CSN EMGMKSVDRL+EE HGNERELR+KASR+S+G  N V
Sbjct: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  ISSLNSSKKS-KNCAQSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSPFFARTHLL 240
            I+SL+S+K S KN A SHLSACA LYLAIV KIEKNE+VSAKH+LQVF DSP+FARTHLL
Sbjct: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240

Query: 241  PELWEHFFLPHLLHLKVWYNQELEFMSN------DRKIKALSKLYNDHMNRGTLKFALYY 300
            P+LWEHFFLPHLLHLKVWYNQELEF+SN      DRKIKAL+K+YNDHM+RGT++FALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300

Query: 301  EQQLNDGAKAPHVPLVPLPSQSIKRASRKSLDS-----SNNKNLYHAVFGPNLDQQLAE- 360
             Q L DGA+AP VP+VP PS+SI +ASR+S DS     S+NKNLYHAVFGP+LDQQLAE 
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  --------------------------KIASVHDEH-------------------HETVKK 420
                                        ASV DEH                    ETVK 
Sbjct: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVK- 420

Query: 421  SDYFR---------------NLET----------NHLLSNELCKAISTICSSDILSECEI 480
            SDYFR               N+ T          NHLLSN+  KAI+ ICSSDILSECEI
Sbjct: 421  SDYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEI 480

Query: 481  AIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEA 540
            A+RVV+KAWLD  GD+ IEVALS PP VEGMLEVLLAS+DDEILELVIS+LAELAARSE 
Sbjct: 481  AVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEI 540

Query: 541  IRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGS 600
            I+ MILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGG 
Sbjct: 541  IQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQ 600

Query: 601  LQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERK 660
            LQ LFSV+C P+EAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLR LE GEIEERK
Sbjct: 601  LQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERK 660

Query: 661  NTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTR 720
            N+VS+ISCCI+ADG+CRNYLAENLNKA+LLELVVHESNKNSDR GLALLV+LLCLSRRTR
Sbjct: 661  NSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTR 720

Query: 721  ITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEA 780
            ITKLLD LKE  +G  IMNILSV L RA PEEQPLVAT LLQLD M+D LNC SIFREEA
Sbjct: 721  ITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNC-SIFREEA 780

Query: 781  IVTIITALNSQISREKTSENLARALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYS 840
            IVTIITALN++I  EK  +NLARALLILGGRFSCTGEP  E WLL+ AGF+ NSGDSS+S
Sbjct: 781  IVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHS 840

Query: 841  SRHVYDDLVQSYEEEEDLVNWQLKASTVFFNHGHKSLLSALSTSMTNGI-PRLAKASLVT 900
             +H+YDD+VQ YEEEE+++NWQLKA++V FNHGHKSLLS+LSTSMT+ I P LAKA L+T
Sbjct: 841  -KHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLIT 900

Query: 901  VSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHI 927
            +SWMSRYLFVIR+EKLC MAPSILVP LIKYLN+DK VED+VLASY+LLNL KYTECKHI
Sbjct: 901  LSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHI 960

BLAST of Cp4.1LG20g05970 vs. TAIR 10
Match: AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 3.9e-22
Identity = 110/430 (25.58%), Postives = 204/430 (47.44%), Query Frame = 0

Query: 484 KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSV-----RCAPYEAAFYLLDQ 543
           +A+IL+YL KP   ++ S+E LP ++ V+    S    ++         P  A+  +++ 
Sbjct: 544 EAAILIYLIKPSPTEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAASLMIIEV 603

Query: 544 LLKGFDEDRNLENGRHLIALGGLSL---LLRTLENGEIEERKNTVSMISCCIRADGTCRN 603
           L+  FD   +  N  HL A+   S+   LL   ++G   E  +  S++  C++ DG  R 
Sbjct: 604 LITAFD---HATNTMHLAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGLHRK 663

Query: 604 YLAENLNKAALLELVVHESNKNSDRC-GLALLVELLCLSRRTRITKLLDELKERSNGSSI 663
           Y+ ++   A    L+  +S    + C  L  L E+L + R + I K+L ++K+  +    
Sbjct: 664 YIYQHTRVAPFAHLL--QSKDQEEICIALQFLHEVLKIPRSSAI-KILQQIKKEGSFDIK 723

Query: 664 MNILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKT 723
           + +L    H  + + +   A ILLQL+ +D P      +R EA   ++ A+    S    
Sbjct: 724 VTLLHCIKH-LQGDHKLFAADILLQLNALDSPPE-NKKYRNEATRALLDAVT--YSEGSN 783

Query: 724 SENLARALLI-LGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEE 783
            + L+  +L  +GG +S TGEP    WL+++ G  + S  +   + +  D+ +Q    + 
Sbjct: 784 MQLLSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMSHMNMIRNINWSDECLQ----DT 843

Query: 784 DLVNWQLKASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLC 843
            +  W  K +    + G K+    L   + +    ++KA L+ ++W+S  +       L 
Sbjct: 844 GIDGWCCKIARRIIDTG-KATFCGLQEGLKSKNKSVSKACLIAIAWLSIEISK-GPNSLK 903

Query: 844 SMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGL--FDEEARDHLENL 902
             A  +L+  + ++L+   ++E+R+LA   + N   ++  K I  L  F E  R+ L  L
Sbjct: 904 YSACEVLLDEVAQFLHPGLELEERLLACICIYN---FSSGKGIHKLVNFSEGVRESLRRL 954

BLAST of Cp4.1LG20g05970 vs. TAIR 10
Match: AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 3.9e-22
Identity = 110/430 (25.58%), Postives = 204/430 (47.44%), Query Frame = 0

Query: 484 KASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSV-----RCAPYEAAFYLLDQ 543
           +A+IL+YL KP   ++ S+E LP ++ V+    S    ++         P  A+  +++ 
Sbjct: 544 EAAILIYLIKPSPTEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAASLMIIEV 603

Query: 544 LLKGFDEDRNLENGRHLIALGGLSL---LLRTLENGEIEERKNTVSMISCCIRADGTCRN 603
           L+  FD   +  N  HL A+   S+   LL   ++G   E  +  S++  C++ DG  R 
Sbjct: 604 LITAFD---HATNTMHLAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGLHRK 663

Query: 604 YLAENLNKAALLELVVHESNKNSDRC-GLALLVELLCLSRRTRITKLLDELKERSNGSSI 663
           Y+ ++   A    L+  +S    + C  L  L E+L + R + I K+L ++K+  +    
Sbjct: 664 YIYQHTRVAPFAHLL--QSKDQEEICIALQFLHEVLKIPRSSAI-KILQQIKKEGSFDIK 723

Query: 664 MNILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKT 723
           + +L    H  + + +   A ILLQL+ +D P      +R EA   ++ A+    S    
Sbjct: 724 VTLLHCIKH-LQGDHKLFAADILLQLNALDSPPE-NKKYRNEATRALLDAVT--YSEGSN 783

Query: 724 SENLARALLI-LGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEE 783
            + L+  +L  +GG +S TGEP    WL+++ G  + S  +   + +  D+ +Q    + 
Sbjct: 784 MQLLSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMSHMNMIRNINWSDECLQ----DT 843

Query: 784 DLVNWQLKASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLC 843
            +  W  K +    + G K+    L   + +    ++KA L+ ++W+S  +       L 
Sbjct: 844 GIDGWCCKIARRIIDTG-KATFCGLQEGLKSKNKSVSKACLIAIAWLSIEISK-GPNSLK 903

Query: 844 SMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGL--FDEEARDHLENL 902
             A  +L+  + ++L+   ++E+R+LA   + N   ++  K I  L  F E  R+ L  L
Sbjct: 904 YSACEVLLDEVAQFLHPGLELEERLLACICIYN---FSSGKGIHKLVNFSEGVRESLRRL 954