Cp4.1LG20g05920 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g05920 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 8.5e-301
Identity = 511/884 (57.81%), Postives = 651/884 (73.64%), Query Frame = 0

Query: 60  AAGEQRENSAVGYGYRVRSGRVDP-TGKSLTADLDLIEKSSVYGPDVERLSIQASFETKD 119
           +  E      +GYGY+V+S +VD  T +SLTA   L++ SSVYGPD++ LSI AS E+ D
Sbjct: 26  STSENDPEGVIGYGYKVKSVKVDSGTRRSLTALPQLVKNSSVYGPDIQLLSITASLESND 85

Query: 120 RLRVRITDSNRERWEVPDEIIPRRS-----NSRIRSLPERDVGSPETS----VISDPASD 179
           RLRVRITD+   RWE+PD I+ R          + SL    + SP T+    ++S P SD
Sbjct: 86  RLRVRITDAKHRRWEIPDNILHRHQPPPPPPHSLSSLYRTLLSSPTTNRRKILLSHPNSD 145

Query: 180 LIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFG 239
           L FSL +T PFGF++ R+S+ DVLFD +P+ ++  TFL+F DQY+ L+SSLP  R+ ++G
Sbjct: 146 LTFSLINTTPFGFTISRKSTHDVLFDATPDPTNPNTFLIFIDQYLHLTSSLPGTRAHIYG 205

Query: 240 IGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTH 299
           +GE ++ +F+L    ++TLT+  ADI S N DVNLYG+HPFY+DVRS        AG+TH
Sbjct: 206 LGEHSKPTFQLA--HNQTLTMRAADIPSSNPDVNLYGSHPFYMDVRS-----SPVAGSTH 265

Query: 300 GVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSF 359
           GVLLLNSNGMD+ Y+G+RI+YK IGGIIDLYFFAGPSP  V++Q+T +IGRPAP+PYW+F
Sbjct: 266 GVLLLNSNGMDVEYTGNRITYKVIGGIIDLYFFAGPSPGQVVEQFTRVIGRPAPMPYWAF 325

Query: 360 GFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNF 419
           GF QCRYGY +V ++++VVA YAKA IPLEVMWTDIDYMD YKDFT DP+NFP++KMK F
Sbjct: 326 GFQQCRYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYKDFTLDPVNFPLDKMKKF 385

Query: 420 VDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFL 479
           V+NLH+NGQKYV+ILDPGISTNKTY TYIRG++ D+F+K +G PYLG VWPGPVYFPDFL
Sbjct: 386 VNNLHKNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKRNGKPYLGSVWPGPVYFPDFL 445

Query: 480 HPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRP 539
            P++  FW DEIK F +++P DGLWIDMNEISNFI+S     S LDNPPYKINN+GV  P
Sbjct: 446 KPSALTFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPIPGSTLDNPPYKINNSGVMLP 505

Query: 540 INNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYT 599
           I N+T+P +++H+G++ EYN HNL+G+LE++ T A+L+K+T KRPFVLSRSTF GSGKYT
Sbjct: 506 IINKTIPPTAMHYGDIPEYNVHNLFGYLEARVTRAALIKLTEKRPFVLSRSTFSGSGKYT 565

Query: 600 AHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR----------- 659
           AHWTGDNAATWNDL Y+IPS+L+FGLFGIPMVGADICGF  +TTEELCR           
Sbjct: 566 AHWTGDNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFLGNTTEELCRRWIQLGAFYPF 625

Query: 660 -------------------LAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSF 719
                              +AASARKVL LRY+LLPY YTLMYEA   G PIARPLFFSF
Sbjct: 626 SRDHSSLGTTYQELYRWESVAASARKVLGLRYTLLPYFYTLMYEAQLNGIPIARPLFFSF 685

Query: 720 PQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQIT 779
           P DI+TY I+SQFLLG GV++SPVLK G +SV AYFP GNWF LF+Y+ SV  ++G+ +T
Sbjct: 686 PDDIKTYGISSQFLLGKGVMVSPVLKPGVVSVTAYFPRGNWFDLFDYTRSVTASTGRYVT 745

Query: 780 LDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVE 839
           L AP DHINVH++EGNILA+ G+A TTQAARKT F LLVV+S+  +S GE+FLDDG  V 
Sbjct: 746 LSAPPDHINVHIQEGNILAMQGKAMTTQAARKTPFHLLVVMSDCGASFGELFLDDGVEVT 805

Query: 840 MGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGL 899
           MG   G W+ V+F + +     +I S V++G FA+SQK +IDKVTI+G  +   + G  +
Sbjct: 806 MGVNRGKWTFVKFIAASAKQTCIITSDVVSGEFAVSQKWVIDKVTILGLRKGTKINGYTV 865

Query: 900 DISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVMEL 904
                      S ++ T +   +F+  EISGL++ +  EF + L
Sbjct: 866 RTGAVTRKGDKSKLKSTPDRKGEFIVAEISGLNLLLGREFKLVL 902

BLAST of Cp4.1LG20g05920 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 5.3e-295
Identity = 501/902 (55.54%), Postives = 659/902 (73.06%), Query Frame = 0

Query: 41  AVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVD-PTGKSLTADLDLIEKSS 100
           AVVL L  C     A+    +    +  A+GYGY+V++ +VD  TGKSLTA L LI  S 
Sbjct: 15  AVVLPLVLCMVVEGAT---TSKNDNQGEAIGYGYQVKNAKVDNSTGKSLTALLQLIRNSP 74

Query: 101 VYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSN-------SRIRSLPE 160
           VYGPD+  LS  ASFE  D LR+R TD+N  RWE+P+E++PR          S ++ LP+
Sbjct: 75  VYGPDIHFLSFTASFEEDDTLRIRFTDANNRRWEIPNEVLPRPPPPPSPPPLSSLQHLPK 134

Query: 161 R-DVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKD 220
                 P T+V+S P SDL F+L  T PFGF+++R+S+ DVLFD +P  S+  TFL++KD
Sbjct: 135 PIPQNQPTTTVLSHPHSDLAFTLFHTTPFGFTIYRKSTHDVLFDATPIPSNPTTFLIYKD 194

Query: 221 QYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFY 280
           QY+QLSSSLP  ++ L+G+GE T+ +F+L    ++ LTLWNADI S N D+NLYG+HPFY
Sbjct: 195 QYLQLSSSLPAQQAHLYGLGEHTKPTFQLA--HNQILTLWNADIASFNRDLNLYGSHPFY 254

Query: 281 IDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVI 340
           +DVRS         G+THGV LLNSNGMD+ Y+GDRI+YK IGGIIDLY FAG +P  V+
Sbjct: 255 MDVRS-----SPMVGSTHGVFLLNSNGMDVEYTGDRITYKVIGGIIDLYIFAGRTPEMVL 314

Query: 341 DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGY 400
           DQYT+LIGRPAP+PYW+FGFHQCR+GY++V++IETVV +YA+A IPLEVMWTDIDYMD +
Sbjct: 315 DQYTKLIGRPAPMPYWAFGFHQCRWGYRDVNEIETVVDKYAEARIPLEVMWTDIDYMDAF 374

Query: 401 KDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDG 460
           KDFT DP++FP++KM+ FV  LH+NGQ+YV ILDPGI+TNK+YGT+IRG+++++FIK +G
Sbjct: 375 KDFTLDPVHFPLDKMQQFVTKLHRNGQRYVPILDPGINTNKSYGTFIRGMQSNVFIKRNG 434

Query: 461 VPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSF 520
            PYLG VWPGPVY+PDFL P +  FW DEIK FRDI+P DG+WIDMNE SNFITS+ +  
Sbjct: 435 NPYLGSVWPGPVYYPDFLDPAARSFWVDEIKRFRDILPIDGIWIDMNEASNFITSAPTPG 494

Query: 521 SNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTG 580
           S LDNPPYKINN+G + PIN++T+PA+++H+GN+TEYN HNLYGFLES+AT  +LV+   
Sbjct: 495 STLDNPPYKINNSGGRVPINSKTIPATAMHYGNVTEYNAHNLYGFLESQATREALVRPAT 554

Query: 581 KRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRD 640
           + PF+LSRSTF GSGKYTAHWTGDNAA W+DL Y+IP++LNFGLFG+PM+GADICGF+  
Sbjct: 555 RGPFLLSRSTFAGSGKYTAHWTGDNAARWDDLQYSIPTMLNFGLFGMPMIGADICGFAES 614

Query: 641 TTEELC------------------------------RLAASARKVLALRYSLLPYIYTLM 700
           TTEELC                               +AASAR VL LRY LLPY YTLM
Sbjct: 615 TTEELCCRWIQLGAFYPFSRDHSARDTTHQELYLWESVAASARTVLGLRYELLPYYYTLM 674

Query: 701 YEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWF 760
           Y+A+ +G+PIARPL F+FP D+ TY I+SQFL+G G+++SPVL+ G+  V+AY P GNW 
Sbjct: 675 YDANLRGSPIARPLSFTFPDDVATYGISSQFLIGRGIMVSPVLQPGSSIVNAYSPRGNWV 734

Query: 761 SLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVS 820
           SL NY+ SV+V++G  ++L AP DHINVH+ EGNI+A+ GEA TTQAAR T F LLVV+S
Sbjct: 735 SLSNYTSSVSVSAGTYVSLSAPPDHINVHIHEGNIVAMQGEAMTTQAARSTPFHLLVVMS 794

Query: 821 NGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIID 880
           +  +S+GE+FLD+G  +++G  GG W+LVRF++E+  + L I S+V+N G+A+SQ+ ++D
Sbjct: 795 DHVASTGELFLDNGIEMDIGGPGGKWTLVRFFAESGINNLTISSEVVNRGYAMSQRWVMD 854

Query: 881 KVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVM 904
           K+TI+G +R   +    +    GA      G R +      F    IS L   + + F +
Sbjct: 855 KITILGLKRRVKIKEYTVQKDAGAIKVKGLGRRTSSHNQGGFFVSVISDLRQLVGQAFKL 906

BLAST of Cp4.1LG20g05920 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 4.2e-268
Identity = 460/840 (54.76%), Postives = 605/840 (72.02%), Query Frame = 0

Query: 102 GPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETS 161
           GPDV RLS+ AS ET  RL VRITD++  RWEVP ++IPR S     +      G     
Sbjct: 65  GPDVRRLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPSPDSFLAATRPGGG----R 124

Query: 162 VISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSL- 221
           V+S   SDL F++H T+PF F+V RRS+GDVLFDT+PN       LVFKD+Y++L+SSL 
Sbjct: 125 VLSTATSDLTFAIH-TSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRYLELTSSLP 184

Query: 222 PKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRD 281
           P  R+SL+G+GEQT+++F+L   ++ T TLWN+DI + N D+NLYG+HPFY+DVRS    
Sbjct: 185 PPGRASLYGLGEQTKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDVRSGGGG 244

Query: 282 GKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGR 341
           G    G  HGVLLLNSNGMD+IY G  ++YK IGG++D YFFAGPSP++V+DQYT+LIGR
Sbjct: 245 G---GGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQYTQLIGR 304

Query: 342 PAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPIN 401
           PAP+PYWSFGFHQCRYGYKNV+D+E VVA YAKA IPLEVMWTDIDYMD YKDFT DP+N
Sbjct: 305 PAPMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDFTLDPVN 364

Query: 402 FPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWP 461
           FP ++M+ FVD LH+NGQK+V+I+DPGI+ N TYGT++RG++ DIF+K++G  YLG VWP
Sbjct: 365 FPADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNYLGVVWP 424

Query: 462 GPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYK 521
           G VYFPDFL+P +  FW  EI  FR  +P DGLW+DMNEISNF+       + +D+PPY+
Sbjct: 425 GNVYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLNAIDDPPYR 484

Query: 522 INNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRS 581
           INN+GV+RPINN+TVPAS++H+G + EY+ HNL+GFLE++ATH +L++ TG+RPFVLSRS
Sbjct: 485 INNSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRPFVLSRS 544

Query: 582 TFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELC--- 641
           TFVGSG+YTAHWTGDNAATW DL Y+I ++L+FGLFGIPM+GADICGF  +TTEELC   
Sbjct: 545 TFVGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTEELCSRW 604

Query: 642 ---------------------------RLAASARKVLALRYSLLPYIYTLMYEAHKKGTP 701
                                       +A SARK L LRY LLPY+YTLMYEAH  G P
Sbjct: 605 IQLGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEAHTTGAP 664

Query: 702 IARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESV 761
           IARPLFFS+P D+ TY I+ QFLLG GVL+SPVL+ GA +V AYFPAG WFSL+++S +V
Sbjct: 665 IARPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLYDFSLAV 724

Query: 762 AVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEV 821
           A  +G+++TL APAD +NVHV  GNIL L   A T+   R++   LLV +++  +++G++
Sbjct: 725 ATKTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDGTATGDL 784

Query: 822 FLDDGEVVEMGEEGGNWSLVRF--YSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGF 881
           FLDDGE  EM      WS ++F   +E+ G  + ++S V++  +A S+ M I KV ++G 
Sbjct: 785 FLDDGESPEMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAIGKVVLMGL 844

Query: 882 ER---PKNMG--GLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVMEL 904
                PK       G+ ++    + G +G   + E  A  V   +SGL++ + +EF +++
Sbjct: 845 RSAAPPKGFAVYANGVQVNASTAVGGAAG---SPEKGALGV-AHVSGLTLVVGQEFDLKV 881

BLAST of Cp4.1LG20g05920 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 3.4e-257
Identity = 442/838 (52.74%), Postives = 587/838 (70.05%), Query Frame = 0

Query: 104 DVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRS-NSRIRSLPERDVGSPETSV 163
           DV+RL++ AS ET  RLRVRITD++  RWEVP +IIPR +    +   P       +  V
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPASSAPLQGRV 118

Query: 164 ISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPK 223
           +S   SDL+ ++H  +PF F+V RRS+GD LFDT+P        LVF+D+Y++++S+LP 
Sbjct: 119 LSPAGSDLVLTVH-ASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSALPA 178

Query: 224 DRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGK 283
            R+SL+G+GE T+ SF+L  + S   TLWNADIG+   DVNLYG+HPFY+DVR+P     
Sbjct: 179 GRASLYGLGEHTKSSFRLRHNDS--FTLWNADIGASYVDVNLYGSHPFYMDVRAP----- 238

Query: 284 VAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPA 343
              GT HGVLLL+SNGMD++Y G  ++YK IGG++D YFFAGP+P++V+DQYT+LI RPA
Sbjct: 239 ---GTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPA 298

Query: 344 PVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFP 403
           P+PYWSFGFHQCRYGY NVSD+E VVARYAKA IPLEVMWTDIDYMDG+KDFT D +NF 
Sbjct: 299 PMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFT 358

Query: 404 VEKMKNFVDNLHQNGQKYVLILDPGIST---NKTYGTYIRGIEADIFIKYDGVPYLGEVW 463
             +++ FVD LH+N QKYVLILDPGI     + TYGT++RG++ DIF+K +G  ++G VW
Sbjct: 359 AAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNFVGNVW 418

Query: 464 PGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPY 523
           PG VYFPDF+HP +  FW  EI LFR  +P DGLWIDMNEISNF   +    + LD+PPY
Sbjct: 419 PGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNALDDPPY 478

Query: 524 KINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSR 583
           +INN G  RPINN+TV   ++H+G +TEY  HNL+G LE++AT   +++ TG+RPFVLSR
Sbjct: 479 RINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRPFVLSR 538

Query: 584 STFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELC-- 643
           STFVGSG+YTA+WTGDNAATW DL Y+I ++L+FGLFG+PM+GADICGF+ +TTEELC  
Sbjct: 539 STFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGR 598

Query: 644 ----------------------------RLAASARKVLALRYSLLPYIYTLMYEAHKKGT 703
                                        +AAS RK L LRY LLPY YTLMYEAH  G 
Sbjct: 599 WIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEAHMTGA 658

Query: 704 PIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSES 763
           PIARPLFFS+P D+ TY ++ QFLLG GVL+SPVL+ G  +VDAYFPAG W+ L++YS +
Sbjct: 659 PIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLYDYSLA 718

Query: 764 VAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGE 823
           VA  +G+ + L APAD +NVH+  G IL L   A TT  AR+TAF LLV ++   ++SG 
Sbjct: 719 VATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDGTASGY 778

Query: 824 VFLDDGEVVEMGEEGGNWSLVRFYSEAVGSK--LLIKSQVINGGFALSQKMIIDKVTIVG 883
           +FLDDG+  E G    +WS+VRF  +   +K  + +KS+V++  +A S+ ++I KV ++G
Sbjct: 779 LFLDDGDSPEYGRR-SDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISKVVLMG 838

Query: 884 FERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNV-EISGLSISIWEEFVMELS 905
              P     L + ++  A ++ +S     Y+ +     V  I GLS+ + EEF ++++
Sbjct: 839 HRSPAAPKKLTVHVN-SAEVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKVA 874

BLAST of Cp4.1LG20g05920 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.2e-217
Identity = 403/944 (42.69%), Postives = 572/944 (60.59%), Query Frame = 0

Query: 22  MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 81
           MA+ S SL   L       +++L L  CF              +    +G GYR+ S   
Sbjct: 1   MASSSSSLAFSL-------SLLLALILCF-----------SPTQSYKTIGKGYRLVSIEE 60

Query: 82  DPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPR 141
            P G      L + +K+ +YG D+  L +    ET  RLRV ITD+ ++RWEVP  ++PR
Sbjct: 61  SPDG-GFIGYLQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPR 120

Query: 142 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 201
               ++  +  +   SP T V     S+LIFS + T PF F+V RRS+ + LF+T+ +  
Sbjct: 121 EQPPQVGKVIGKSRKSPIT-VQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTTSS-- 180

Query: 202 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTR-KSFKLVPDKSKTLTLWNADIGSVNT 261
                LVFKDQY+++S+SLPK+ +SL+G+GE ++    KLVP  ++  TL+  D+ ++N 
Sbjct: 181 -----LVFKDQYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINL 240

Query: 262 DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLY 321
           + +LYG+HP Y+D+R+    GK  A   H VLLLNSNGMD+ Y GD ++YK IGG+ D Y
Sbjct: 241 NTDLYGSHPMYMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFY 300

Query: 322 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEV 381
           F AGPSP++V+DQYT+LIGRPAP+PYWS GFHQCR+GY N+S +E VV  Y KA IPL+V
Sbjct: 301 FIAGPSPLNVVDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDV 360

Query: 382 MWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRG 441
           +W D D+MDG+KDFT +P+ +P  K+  F+D +H+ G KY++I DPGI  N +YGT+ R 
Sbjct: 361 IWNDDDHMDGHKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRA 420

Query: 442 IEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEI 501
           + AD+FIKY+G P+L +VWPGPVYFPDFL+P +  +WGDEIK F D+VP DGLWIDMNE+
Sbjct: 421 MAADVFIKYEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEV 480

Query: 502 SNF------------------------ITSSTSSFSNLDNPPYKINNAGVQRPINNRTVP 561
           SNF                        +     + +  D+PPYKIN  GV  P+  +T+ 
Sbjct: 481 SNFCSGLCTIPEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIA 540

Query: 562 ASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN 621
            S+ H+  + EY+ H++YGF E+ ATH  L+ V GKRPF+LSRSTFVGSG+Y AHWTGDN
Sbjct: 541 TSATHYNGVREYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDN 600

Query: 622 AATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR------------------ 681
             TW  L  +I ++LNFG+FG+PMVG+DICGF    TEELC                   
Sbjct: 601 QGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANY 660

Query: 682 ------------LAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTY 741
                       +A SAR  L +RY +LP++YTL YEAH  G PIARPLFFSFP+    Y
Sbjct: 661 YSPRQELYQWDTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECY 720

Query: 742 EINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADH 801
             + QFLLG+  +ISPVL++G   V+A FP G+W+ +F+ +++V   +G+++TL AP + 
Sbjct: 721 GNSRQFLLGSSFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNF 780

Query: 802 INVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQS---SSGEVFLDDGEVVEMGEE 861
           +NVH+ +  IL        ++ AR T F L++    G S   ++G+++LD+ E+ EM   
Sbjct: 781 VNVHLYQNTILPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLG 840

Query: 862 GGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISK 902
            G  + V FY+      + + SQV  G FALS+  +I+KV+++G      +  + ++ S 
Sbjct: 841 NGQSTYVDFYASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSP 900

BLAST of Cp4.1LG20g05920 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1810 bits (4688), Expect = 0.0
Identity = 906/937 (96.69%), Postives = 907/937 (96.80%), Query Frame = 0

Query: 1   MDSDIFCKKIRRRTHPYSSTTMATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFA 60
           MDSDIFCKKIRRRTHPYSSTTMATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFA
Sbjct: 1   MDSDIFCKKIRRRTHPYSSTTMATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFA 60

Query: 61  AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRL 120
           AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRL
Sbjct: 61  AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRL 120

Query: 121 RVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPF 180
           RVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPF
Sbjct: 121 RVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPF 180

Query: 181 GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKL 240
           GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKL
Sbjct: 181 GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKL 240

Query: 241 VPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD 300
           VPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD
Sbjct: 241 VPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD 300

Query: 301 IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN 360
           IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN
Sbjct: 301 IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN 360

Query: 361 VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKY 420
           VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKY
Sbjct: 361 VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKY 420

Query: 421 VLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDE 480
           VLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDE
Sbjct: 421 VLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDE 480

Query: 481 IKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSL 540
           IKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSL
Sbjct: 481 IKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSL 540

Query: 541 HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW 600
           HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW
Sbjct: 541 HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW 600

Query: 601 NDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR---------------------- 660
           NDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR                      
Sbjct: 601 NDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCRRWIQLGAFYPFARDHSDKGSIR 660

Query: 661 --------LAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS 720
                   +AASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS
Sbjct: 661 QELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS 720

Query: 721 QFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVH 780
           QFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVH
Sbjct: 721 QFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVH 780

Query: 781 VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV 840
           VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV
Sbjct: 781 VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV 840

Query: 841 RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGN 900
           RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGN
Sbjct: 841 RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGN 900

Query: 901 SGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 907
           SGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN
Sbjct: 901 SGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 937

BLAST of Cp4.1LG20g05920 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1764 bits (4570), Expect = 0.0
Identity = 880/937 (93.92%), Postives = 895/937 (95.52%), Query Frame = 0

Query: 1   MDSDIFCKKIRRRTHPYSSTTMATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFA 60
           MDSDIFCK+IRRR HPY STTMATGSKSLQAFLRRRRLLSAVVLFLF+CFFRL ASLPFA
Sbjct: 1   MDSDIFCKEIRRRPHPYISTTMATGSKSLQAFLRRRRLLSAVVLFLFSCFFRLIASLPFA 60

Query: 61  AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRL 120
           AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLS+QASFETKDRL
Sbjct: 61  AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRL 120

Query: 121 RVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPF 180
           RVRITDSNRERWE+PDEIIPRRSNSRIRSLPERDVGSPETS+ISDPASDLIFSLHDTAPF
Sbjct: 121 RVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPF 180

Query: 181 GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKL 240
           GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFK+
Sbjct: 181 GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKI 240

Query: 241 VPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD 300
           VPDKSKTLTLW+ADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD
Sbjct: 241 VPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD 300

Query: 301 IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN 360
           IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN
Sbjct: 301 IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN 360

Query: 361 VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKY 420
           VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVD+LHQNGQKY
Sbjct: 361 VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKY 420

Query: 421 VLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDE 480
           VLI+DPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWG E
Sbjct: 421 VLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGE 480

Query: 481 IKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSL 540
           IKLFRDIVPFDGLWIDMNE+SNFITSSTS FSNLDNPPYKI+NAGVQRPINNRTVPASSL
Sbjct: 481 IKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSL 540

Query: 541 HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW 600
           HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW
Sbjct: 541 HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW 600

Query: 601 NDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR---------------------- 660
           NDLGYTIPSILNFGLFGIPMVGADICGFS DTTEELCR                      
Sbjct: 601 NDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIR 660

Query: 661 --------LAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS 720
                   +AASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS
Sbjct: 661 QELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS 720

Query: 721 QFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVH 780
           QFLLG GVL+SPVLKEGA+SVDAYFPAGNWFSLFNYSESVAV SGQQITLDAPADHINVH
Sbjct: 721 QFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVH 780

Query: 781 VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV 840
           VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV
Sbjct: 781 VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV 840

Query: 841 RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGN 900
           RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVT VGFERPK MGGLGLDISKGANL+GN
Sbjct: 841 RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGN 900

Query: 901 SGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 907
           SGIRKTYEYSAKFVNVEISGLSI I EEFVMELSPLN
Sbjct: 901 SGIRKTYEYSAKFVNVEISGLSIPICEEFVMELSPLN 937

BLAST of Cp4.1LG20g05920 vs. NCBI nr
Match: KAG6583815.1 (hypothetical protein SDJN03_19747, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019441.1 hypothetical protein SDJN02_18402 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1738 bits (4500), Expect = 0.0
Identity = 867/916 (94.65%), Postives = 878/916 (95.85%), Query Frame = 0

Query: 22  MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 81
           MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGE+RENSAVGYGYRVRSGRV
Sbjct: 1   MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEKRENSAVGYGYRVRSGRV 60

Query: 82  DPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPR 141
           DPTGKSLTADLDLIEKSSVYGPDVERLS+QASFETKDRLRVRITDSNRERWE+PDEIIPR
Sbjct: 61  DPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEIPDEIIPR 120

Query: 142 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 201
           RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS
Sbjct: 121 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 180

Query: 202 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD 261
           D ETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD
Sbjct: 181 DLETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD 240

Query: 262 VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYF 321
           VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIY+GDRISYKAIGGIIDLYF
Sbjct: 241 VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYF 300

Query: 322 FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM 381
           FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVV+RYAKAGIPLEVM
Sbjct: 301 FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVSRYAKAGIPLEVM 360

Query: 382 WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI 441
           WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI
Sbjct: 361 WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI 420

Query: 442 EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEIS 501
           EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFR+IVPFDGLWIDMNEIS
Sbjct: 421 EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFREIVPFDGLWIDMNEIS 480

Query: 502 NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA 561
           NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA
Sbjct: 481 NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA 540

Query: 562 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV 621
           THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV
Sbjct: 541 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV 600

Query: 622 GADICGFSRDTTEELCR------------------------------LAASARKVLALRY 681
           GADICGFS DTTEELCR                              +AASARKVLALRY
Sbjct: 601 GADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRY 660

Query: 682 SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV 741
           SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV
Sbjct: 661 SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV 720

Query: 742 DAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARK 801
           DAYFPAGNWFSLFNYSESVA+ SGQQITLDAPADHINVHVREGNILALHGEATTTQAARK
Sbjct: 721 DAYFPAGNWFSLFNYSESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARK 780

Query: 802 TAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG 861
           T FKLLVVVSNGQSSSGEVFLDDGE +EMGEEGGNWSLVRFY EAVGSKLLIKSQVINGG
Sbjct: 781 TEFKLLVVVSNGQSSSGEVFLDDGEEMEMGEEGGNWSLVRFYGEAVGSKLLIKSQVINGG 840

Query: 862 FALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGL 907
           FALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANL+GNSGIRKTYE+SAKFVNVEISGL
Sbjct: 841 FALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLNGNSGIRKTYEHSAKFVNVEISGL 900

BLAST of Cp4.1LG20g05920 vs. NCBI nr
Match: XP_022927400.1 (alpha-glucosidase [Cucurbita moschata])

HSP 1 Score: 1728 bits (4476), Expect = 0.0
Identity = 867/916 (94.65%), Postives = 875/916 (95.52%), Query Frame = 0

Query: 22  MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 81
           MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV
Sbjct: 1   MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 60

Query: 82  DPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPR 141
           DPTGKSLTADLDLI+KSSVYGPDVERLS+QASFETKDRLRVRITDSNRERWEVPDEIIPR
Sbjct: 61  DPTGKSLTADLDLIDKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDEIIPR 120

Query: 142 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 201
           RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS
Sbjct: 121 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 180

Query: 202 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD 261
           DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD
Sbjct: 181 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD 240

Query: 262 VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYF 321
           VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIY+GDRISYKAIGGIIDLYF
Sbjct: 241 VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYF 300

Query: 322 FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM 381
           FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM
Sbjct: 301 FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM 360

Query: 382 WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI 441
           WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI
Sbjct: 361 WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI 420

Query: 442 EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEIS 501
           EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKL RDIVPFDGLWIDMNEIS
Sbjct: 421 EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEIS 480

Query: 502 NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA 561
           NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA
Sbjct: 481 NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA 540

Query: 562 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV 621
           THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV
Sbjct: 541 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV 600

Query: 622 GADICGFSRDTTEELCR------------------------------LAASARKVLALRY 681
           GADICGFS DTTEELCR                              +AASARKVLALRY
Sbjct: 601 GADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRY 660

Query: 682 SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV 741
           SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV
Sbjct: 661 SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV 720

Query: 742 DAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARK 801
           DAYFPAGNWFSLFNYSESVA+ SGQQITLDAPADHINVHVREGNILALHGEATTTQAARK
Sbjct: 721 DAYFPAGNWFSLFNYSESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARK 780

Query: 802 TAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG 861
           T FKLLVVVSNGQSSSGEVFLDDGE +EMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG
Sbjct: 781 TEFKLLVVVSNGQSSSGEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG 840

Query: 862 FALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGL 907
           FALSQKMIIDKVTIVGFERPKNMG LGLDISKGANL+GNSGIR TYEYSAKFVNV+ISGL
Sbjct: 841 FALSQKMIIDKVTIVGFERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKFVNVQISGL 900

BLAST of Cp4.1LG20g05920 vs. NCBI nr
Match: XP_038894574.1 (alpha-glucosidase [Benincasa hispida])

HSP 1 Score: 1496 bits (3873), Expect = 0.0
Identity = 749/920 (81.41%), Postives = 803/920 (87.28%), Query Frame = 0

Query: 22  MATGSKSLQA----FLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVR 81
           M +GSK+++      L+   L  A++ FLF  FF         AG     SAVGYGYR+R
Sbjct: 1   MGSGSKAMRTPFSQSLQPPLLFPALLFFLFPSFFP-----NLVAGSL---SAVGYGYRIR 60

Query: 82  SGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDE 141
           S  VDP GKSLTADLDLI  S V GPDV RL++QA+FETKDRLR+RITDS RERWE+PD+
Sbjct: 61  SSHVDPAGKSLTADLDLIRTSPVNGPDVPRLTLQATFETKDRLRIRITDSTRERWEIPDQ 120

Query: 142 IIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTS 201
           IIPRRS+SRIRSLPE  VGSP TS ISDPASDLIF+LH TAPFGFSV RRSSGDVLFDTS
Sbjct: 121 IIPRRSSSRIRSLPENHVGSPRTSFISDPASDLIFTLHYTAPFGFSVLRRSSGDVLFDTS 180

Query: 202 PNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGS 261
           P+ SDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFKL+PDK+KTLTLWNADIGS
Sbjct: 181 PDLSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLMPDKNKTLTLWNADIGS 240

Query: 262 VNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGII 321
           VN DVNLYGAHPFYIDVRSPS DGKVAAGTTHGVLLLNSNGMDIIYSG RI YK IGGII
Sbjct: 241 VNLDVNLYGAHPFYIDVRSPSGDGKVAAGTTHGVLLLNSNGMDIIYSGHRIMYKVIGGII 300

Query: 322 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIP 381
           DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSD+E+VVARYAKAGIP
Sbjct: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDLESVVARYAKAGIP 360

Query: 382 LEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTY 441
           LEVMWTDIDYMDGYKDFTFDP+NFP EKMK FVDNLH+NGQKYVLILDPGISTN TYGT+
Sbjct: 361 LEVMWTDIDYMDGYKDFTFDPVNFPAEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTF 420

Query: 442 IRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDM 501
           IRG  ADIFI+YDGVPYLGEVWPGPVYFPDFLHPNSE FWG EI+LFRDIVPFDGLWIDM
Sbjct: 421 IRGKRADIFIQYDGVPYLGEVWPGPVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDM 480

Query: 502 NEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFL 561
           NEISNFITSSTS FSNLDNPPY INNAGV+RP+NN+TVPAS LHFGNLTEYNTHNLYGFL
Sbjct: 481 NEISNFITSSTSPFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFL 540

Query: 562 ESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFG 621
           ESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDL  TIPSILNFGLFG
Sbjct: 541 ESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLRNTIPSILNFGLFG 600

Query: 622 IPMVGADICGFSRDTTEELC------------------------------RLAASARKVL 681
           IPMVGADICGFS DTTEELC                               +AASARKVL
Sbjct: 601 IPMVGADICGFSGDTTEELCGRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVL 660

Query: 682 ALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEG 741
           ALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TY INSQFLLG GVL+SPVLKEG
Sbjct: 661 ALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYGINSQFLLGEGVLVSPVLKEG 720

Query: 742 AISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQ 801
           A+SVDAYFP GNWFSLFNYSE VAV SGQ+ITLDAPADHINVHVREGNILALHG+A TT+
Sbjct: 721 AVSVDAYFPTGNWFSLFNYSELVAVKSGQRITLDAPADHINVHVREGNILALHGKAMTTR 780

Query: 802 AARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQV 861
           AAR+TA++LLVVVSN QSS GEVFLDDGEVVEMG EGGNWSLVRFYSEAVGSKL++KSQV
Sbjct: 781 AARETAYELLVVVSNDQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEAVGSKLVVKSQV 840

Query: 862 INGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVE 907
           INGGFALSQ +IIDKVT VGFERPK M  LGL+ISKG  L+GNS IRKTY+Y AK +NVE
Sbjct: 841 INGGFALSQNLIIDKVTFVGFERPKKMVDLGLNISKGVKLNGNSTIRKTYKYFAKVMNVE 900

BLAST of Cp4.1LG20g05920 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1764 bits (4570), Expect = 0.0
Identity = 880/937 (93.92%), Postives = 895/937 (95.52%), Query Frame = 0

Query: 1   MDSDIFCKKIRRRTHPYSSTTMATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFA 60
           MDSDIFCK+IRRR HPY STTMATGSKSLQAFLRRRRLLSAVVLFLF+CFFRL ASLPFA
Sbjct: 1   MDSDIFCKEIRRRPHPYISTTMATGSKSLQAFLRRRRLLSAVVLFLFSCFFRLIASLPFA 60

Query: 61  AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRL 120
           AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLS+QASFETKDRL
Sbjct: 61  AGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRL 120

Query: 121 RVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPF 180
           RVRITDSNRERWE+PDEIIPRRSNSRIRSLPERDVGSPETS+ISDPASDLIFSLHDTAPF
Sbjct: 121 RVRITDSNRERWEIPDEIIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPF 180

Query: 181 GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKL 240
           GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFK+
Sbjct: 181 GFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKI 240

Query: 241 VPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD 300
           VPDKSKTLTLW+ADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD
Sbjct: 241 VPDKSKTLTLWSADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMD 300

Query: 301 IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN 360
           IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN
Sbjct: 301 IIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKN 360

Query: 361 VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKY 420
           VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVD+LHQNGQKY
Sbjct: 361 VSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKY 420

Query: 421 VLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDE 480
           VLI+DPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWG E
Sbjct: 421 VLIVDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGE 480

Query: 481 IKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSL 540
           IKLFRDIVPFDGLWIDMNE+SNFITSSTS FSNLDNPPYKI+NAGVQRPINNRTVPASSL
Sbjct: 481 IKLFRDIVPFDGLWIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSL 540

Query: 541 HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW 600
           HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW
Sbjct: 541 HFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATW 600

Query: 601 NDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR---------------------- 660
           NDLGYTIPSILNFGLFGIPMVGADICGFS DTTEELCR                      
Sbjct: 601 NDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIR 660

Query: 661 --------LAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS 720
                   +AASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS
Sbjct: 661 QELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINS 720

Query: 721 QFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVH 780
           QFLLG GVL+SPVLKEGA+SVDAYFPAGNWFSLFNYSESVAV SGQQITLDAPADHINVH
Sbjct: 721 QFLLGEGVLVSPVLKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVH 780

Query: 781 VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV 840
           VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV
Sbjct: 781 VREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLV 840

Query: 841 RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGN 900
           RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVT VGFERPK MGGLGLDISKGANL+GN
Sbjct: 841 RFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGN 900

Query: 901 SGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 907
           SGIRKTYEYSAKFVNVEISGLSI I EEFVMELSPLN
Sbjct: 901 SGIRKTYEYSAKFVNVEISGLSIPICEEFVMELSPLN 937

BLAST of Cp4.1LG20g05920 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1728 bits (4476), Expect = 0.0
Identity = 867/916 (94.65%), Postives = 875/916 (95.52%), Query Frame = 0

Query: 22  MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 81
           MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV
Sbjct: 1   MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 60

Query: 82  DPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPR 141
           DPTGKSLTADLDLI+KSSVYGPDVERLS+QASFETKDRLRVRITDSNRERWEVPDEIIPR
Sbjct: 61  DPTGKSLTADLDLIDKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDEIIPR 120

Query: 142 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 201
           RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS
Sbjct: 121 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 180

Query: 202 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD 261
           DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD
Sbjct: 181 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTD 240

Query: 262 VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYF 321
           VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIY+GDRISYKAIGGIIDLYF
Sbjct: 241 VNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYF 300

Query: 322 FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM 381
           FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM
Sbjct: 301 FAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVM 360

Query: 382 WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI 441
           WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI
Sbjct: 361 WTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGI 420

Query: 442 EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEIS 501
           EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKL RDIVPFDGLWIDMNEIS
Sbjct: 421 EADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEIS 480

Query: 502 NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA 561
           NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA
Sbjct: 481 NFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKA 540

Query: 562 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV 621
           THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV
Sbjct: 541 THASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMV 600

Query: 622 GADICGFSRDTTEELCR------------------------------LAASARKVLALRY 681
           GADICGFS DTTEELCR                              +AASARKVLALRY
Sbjct: 601 GADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRY 660

Query: 682 SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV 741
           SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV
Sbjct: 661 SLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISV 720

Query: 742 DAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARK 801
           DAYFPAGNWFSLFNYSESVA+ SGQQITLDAPADHINVHVREGNILALHGEATTTQAARK
Sbjct: 721 DAYFPAGNWFSLFNYSESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARK 780

Query: 802 TAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG 861
           T FKLLVVVSNGQSSSGEVFLDDGE +EMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG
Sbjct: 781 TEFKLLVVVSNGQSSSGEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGG 840

Query: 862 FALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGL 907
           FALSQKMIIDKVTIVGFERPKNMG LGLDISKGANL+GNSGIR TYEYSAKFVNV+ISGL
Sbjct: 841 FALSQKMIIDKVTIVGFERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKFVNVQISGL 900

BLAST of Cp4.1LG20g05920 vs. ExPASy TrEMBL
Match: A0A0A0M3Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G716250 PE=3 SV=1)

HSP 1 Score: 1488 bits (3852), Expect = 0.0
Identity = 743/928 (80.06%), Postives = 805/928 (86.75%), Query Frame = 0

Query: 14  TH-PYSSTTMATGSKSLQAFLRRR--RLLSAVVLFLFTCFFRL-TASLPFAAGEQRENSA 73
           TH P    TMA+GS  +   +R      L  ++LFLFT F  L  ASLP          A
Sbjct: 6   THFPLPQKTMASGSSKV---IRTPFPHSLPILILFLFTSFLPLPAASLP----------A 65

Query: 74  VGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNR 133
           VG GYR+RS  VDP GK+LTADLDLI  S VYGPD+  L++QA+FE+KDRLRVRITDS R
Sbjct: 66  VGLGYRIRSSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTR 125

Query: 134 ERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSS 193
           ERWEVP  I+PR S+S IRSLPE  V SP+ S IS PASDLIF+LHDTAPFGFSV RRSS
Sbjct: 126 ERWEVPHHILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSS 185

Query: 194 GDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLT 253
           GDVLFDTSP FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTRKSFKLVPDK+KTLT
Sbjct: 186 GDVLFDTSPAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLT 245

Query: 254 LWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRIS 313
           LWNADIGSVN DVNLYGAHPFYID+RSPS+DGKVAAGTTHGVLLLNSNGMDI+YSGDRI+
Sbjct: 246 LWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRIT 305

Query: 314 YKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVA 373
           YK IGGIIDLYFFAGPSPISV+DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVA
Sbjct: 306 YKVIGGIIDLYFFAGPSPISVVDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVA 365

Query: 374 RYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGIS 433
           RYAKA IPLE MWTDIDYMDGYKDFTFDPINFP +KMK FVDNLH+NGQKYVLILDPGIS
Sbjct: 366 RYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGIS 425

Query: 434 TNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVP 493
           TN TYG YIRG +ADIF+KY+GVPYLG+VWPGPVYFPDF HP+SE FWG EI++FRDIVP
Sbjct: 426 TNNTYGPYIRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVP 485

Query: 494 FDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYN 553
           FDGLWIDMNEISNFITSSTS  SNLDNPPY INNA VQRP+NN+TVPAS LHFGNLTEYN
Sbjct: 486 FDGLWIDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYN 545

Query: 554 THNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPS 613
           THNLYGFLES+ATHASLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGYTIPS
Sbjct: 546 THNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPS 605

Query: 614 ILNFGLFGIPMVGADICGFSRDTTEELCR------------------------------L 673
           ILNFGLFGIPMVG+DICGFS DTTEELCR                              +
Sbjct: 606 ILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSV 665

Query: 674 AASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVL 733
           AASARKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+T+EI+SQFLLG GVL
Sbjct: 666 AASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVL 725

Query: 734 ISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILAL 793
           +SPVLKEGA SVDAYFPAGNWFSLFNYSE VAVNSGQQI LDAPADHINVHVREGNILAL
Sbjct: 726 VSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILAL 785

Query: 794 HGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGS 853
           HGEA TT+AA++T +KLLVV+SNGQSS GEVFLDDGEVVEMG EGGNWS+VRFYSE VGS
Sbjct: 786 HGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGS 845

Query: 854 KLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEY 907
           KL++KSQVINGGFALSQK+IIDKVT VGF+RPK M  LGL+ISKG NL+GNS IRKTY+Y
Sbjct: 846 KLVVKSQVINGGFALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQY 905

BLAST of Cp4.1LG20g05920 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1479 bits (3829), Expect = 0.0
Identity = 730/898 (81.29%), Postives = 790/898 (87.97%), Query Frame = 0

Query: 42  VVLFLFTCFF--RLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSS 101
           ++LFLFT F      ASLP          AVG+GYR+RS  +DP GKSLTADL LI  S 
Sbjct: 22  LILFLFTSFLIPPPAASLP----------AVGFGYRIRSSHIDPAGKSLTADLQLIRTSQ 81

Query: 102 VYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPE 161
           VYGPD+  L++QA+FE+KDRLRVRITDS RERWE+PD IIPR SNS IRSLPE  V SP+
Sbjct: 82  VYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIRSLPENHVASPK 141

Query: 162 TSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSS 221
            S ISDPASDLIF+L+DTAPFGFSV RRSSGDVLFDTSP+FS+SETF+VFKDQYIQLSS 
Sbjct: 142 PSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIVFKDQYIQLSSL 201

Query: 222 LPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSR 281
           LPKDRSS+FGIGEQTRKSFKLVPDK+KTLTLWNADIGSVN DVNLYGAHPFYIDVRS SR
Sbjct: 202 LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDVRSRSR 261

Query: 282 DGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIG 341
           DGKV AGTTHGVLLLNSNGMDIIYSGDRI+YK IGGIIDLYFFAGPSPISVIDQYTELIG
Sbjct: 262 DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIG 321

Query: 342 RPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPI 401
           RPAPVPYWSFGFHQCRYGYKNVS++E+VVARYAKA IPLEVMWTDIDYMDGYKDFTFDPI
Sbjct: 322 RPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYMDGYKDFTFDPI 381

Query: 402 NFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVW 461
           NFP EKMK FVDNLH+NGQKYV+ILDPGISTN TYG YIRG +ADIF+K+DGVPYLG+VW
Sbjct: 382 NFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMKHDGVPYLGDVW 441

Query: 462 PGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPY 521
           PGPVYFPDFLHP+SE FWG EI++FRDIVPFDGLWIDMNEISNFITSSTS  SNLDNPPY
Sbjct: 442 PGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPY 501

Query: 522 KINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSR 581
            INNA V+RP+NN+TVPAS LHFGNLTEYNTHNLYGFLES+ATHASLVKVTGKRPFVLSR
Sbjct: 502 MINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGKRPFVLSR 561

Query: 582 STFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR- 641
           STF GSGKYTAHWTGD  ATWND+GYTIPSILNFGLFGIPMVG+DICGFS DTTEELCR 
Sbjct: 562 STFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 621

Query: 642 -----------------------------LAASARKVLALRYSLLPYIYTLMYEAHKKGT 701
                                        +AASARKVLALRY LLPY YTLMYEAHKKGT
Sbjct: 622 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 681

Query: 702 PIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSES 761
           PIARPLFFSFPQDI+TYEI+SQFLLG GVL+SPVLKEGA SVDAYFPAGNWFSLFNYSE 
Sbjct: 682 PIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEF 741

Query: 762 VAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGE 821
           VAVNSGQQI LDAPADHINVHVREGNILALHGEA TTQAAR+TA+KLLVV+SNGQ S GE
Sbjct: 742 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVVISNGQRSFGE 801

Query: 822 VFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFE 881
           VFLDDGEV EMG EGGNWS+VRF SE VGSKL++KSQVINGGFALSQK+IIDKVT VGFE
Sbjct: 802 VFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFE 861

Query: 882 RPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 907
           RPK M GLGL+ISKG +L+GNS IRKTY+Y AKF+NVEISGLSI IWEEF++E++P++
Sbjct: 862 RPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMTPIS 909

BLAST of Cp4.1LG20g05920 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1479 bits (3829), Expect = 0.0
Identity = 730/898 (81.29%), Postives = 790/898 (87.97%), Query Frame = 0

Query: 42  VVLFLFTCFF--RLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSS 101
           ++LFLFT F      ASLP          AVG+GYR+RS  +DP GKSLTADL LI  S 
Sbjct: 22  LILFLFTSFLIPPPAASLP----------AVGFGYRIRSSHIDPAGKSLTADLQLIRTSQ 81

Query: 102 VYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGSPE 161
           VYGPD+  L++QA+FE+KDRLRVRITDS RERWE+PD IIPR SNS IRSLPE  V SP+
Sbjct: 82  VYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIRSLPENHVASPK 141

Query: 162 TSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSS 221
            S ISDPASDLIF+L+DTAPFGFSV RRSSGDVLFDTSP+FS+SETF+VFKDQYIQLSS 
Sbjct: 142 PSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIVFKDQYIQLSSL 201

Query: 222 LPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSR 281
           LPKDRSS+FGIGEQTRKSFKLVPDK+KTLTLWNADIGSVN DVNLYGAHPFYIDVRS SR
Sbjct: 202 LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDVRSRSR 261

Query: 282 DGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIG 341
           DGKV AGTTHGVLLLNSNGMDIIYSGDRI+YK IGGIIDLYFFAGPSPISVIDQYTELIG
Sbjct: 262 DGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIG 321

Query: 342 RPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPI 401
           RPAPVPYWSFGFHQCRYGYKNVS++E+VVARYAKA IPLEVMWTDIDYMDGYKDFTFDPI
Sbjct: 322 RPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYMDGYKDFTFDPI 381

Query: 402 NFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVW 461
           NFP EKMK FVDNLH+NGQKYV+ILDPGISTN TYG YIRG +ADIF+K+DGVPYLG+VW
Sbjct: 382 NFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMKHDGVPYLGDVW 441

Query: 462 PGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPY 521
           PGPVYFPDFLHP+SE FWG EI++FRDIVPFDGLWIDMNEISNFITSSTS  SNLDNPPY
Sbjct: 442 PGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPY 501

Query: 522 KINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSR 581
            INNA V+RP+NN+TVPAS LHFGNLTEYNTHNLYGFLES+ATHASLVKVTGKRPFVLSR
Sbjct: 502 MINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGKRPFVLSR 561

Query: 582 STFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR- 641
           STF GSGKYTAHWTGD  ATWND+GYTIPSILNFGLFGIPMVG+DICGFS DTTEELCR 
Sbjct: 562 STFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 621

Query: 642 -----------------------------LAASARKVLALRYSLLPYIYTLMYEAHKKGT 701
                                        +AASARKVLALRY LLPY YTLMYEAHKKGT
Sbjct: 622 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 681

Query: 702 PIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSES 761
           PIARPLFFSFPQDI+TYEI+SQFLLG GVL+SPVLKEGA SVDAYFPAGNWFSLFNYSE 
Sbjct: 682 PIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEF 741

Query: 762 VAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGE 821
           VAVNSGQQI LDAPADHINVHVREGNILALHGEA TTQAAR+TA+KLLVV+SNGQ S GE
Sbjct: 742 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVVISNGQRSFGE 801

Query: 822 VFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFE 881
           VFLDDGEV EMG EGGNWS+VRF SE VGSKL++KSQVINGGFALSQK+IIDKVT VGFE
Sbjct: 802 VFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFE 861

Query: 882 RPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKFVNVEISGLSISIWEEFVMELSPLN 907
           RPK M GLGL+ISKG +L+GNS IRKTY+Y AKF+NVEISGLSI IWEEF++E++P++
Sbjct: 862 RPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMTPIS 909

BLAST of Cp4.1LG20g05920 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1123.2 bits (2904), Expect = 0.0e+00
Identity = 550/896 (61.38%), Postives = 688/896 (76.79%), Query Frame = 0

Query: 43  VLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYG 102
           +  +   FF L +S      E+ E++ VGYGY VRS  VD   + LTA LDLI+ SSVY 
Sbjct: 10  IFIVVVVFFSLRSSQ--VVLEEEESTVVGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVYA 69

Query: 103 PDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSN-SRIRSLPERDVG-SPET 162
           PD++ L++  S ET +RLR+RITDS+++RWE+P+ +IPR  N S  R   E D G SPE 
Sbjct: 70  PDIKSLNLHVSLETSERLRIRITDSSQQRWEIPETVIPRAGNHSPRRFSTEEDGGNSPEN 129

Query: 163 SVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLSSSL 222
           + ++DP+SDL+F+LH+T PFGFSV RRSSGD+LFDTSP+ SDS T+ +FKDQ++QLSS+L
Sbjct: 130 NFLADPSSDLVFTLHNTTPFGFSVSRRSSGDILFDTSPDSSDSNTYFIFKDQFLQLSSAL 189

Query: 223 PKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSPSRD 282
           P++RS+L+GIGE T++SF+L+P   +T+TLWNADIGS N DVNLYG+HPFY+DVR     
Sbjct: 190 PENRSNLYGIGEHTKRSFRLIP--GETMTLWNADIGSENPDVNLYGSHPFYMDVRGSK-- 249

Query: 283 GKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGR 342
           G   AGTTHGVLLLNSNGMD+ Y G RI+Y  IGG+IDLY FAGPSP  V++QYTELIGR
Sbjct: 250 GNEEAGTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPSPEMVMNQYTELIGR 309

Query: 343 PAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPIN 402
           PAP+PYWSFGFHQCRYGYKNVSD+E VV  YAKAGIPLEVMWTDIDYMDGYKDFT DP+N
Sbjct: 310 PAPMPYWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVN 369

Query: 403 FPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGEVWP 462
           FP +KM++FVD LH+NGQKYVLILDPGI  + +YGTY RG+EAD+FIK +G PYLGEVWP
Sbjct: 370 FPEDKMQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGEVWP 429

Query: 463 GPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYK 522
           G VYFPDFL+P +  FW +EIK+F++I+P DGLWIDMNE+SNFITS  SS S+LD+PPYK
Sbjct: 430 GKVYFPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPYK 489

Query: 523 INNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRS 582
           INN+G +RPINN+TVPA+S+HFGN++EY+ HNLYG LE+KATH ++V +TGKRPF+LSRS
Sbjct: 490 INNSGDKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFILSRS 549

Query: 583 TFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR-- 642
           TFV SGKYTAHWTGDNAA W DL Y+IP ILNFGLFGIPMVGADICGFS DTTEELCR  
Sbjct: 550 TFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRRW 609

Query: 643 ----------------------------LAASARKVLALRYSLLPYIYTLMYEAHKKGTP 702
                                       +A+SARKVL LR  LLP++YTLMYEAH  G P
Sbjct: 610 IQLGAFYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGNP 669

Query: 703 IARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESV 762
           IARPLFFSFPQD +TYEI+SQFL+G  +++SP LK+GA++VDAYFPAGNWF LFNYS +V
Sbjct: 670 IARPLFFSFPQDTKTYEIDSQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYSFAV 729

Query: 763 AVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQSSSGEV 822
             +SG+ + LD PADH+NVHVREG+I+A+ GEA TT+ ARKT ++LLVV S  ++ SGE+
Sbjct: 730 GGDSGKHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKTPYQLLVVASRLENISGEL 789

Query: 823 FLDDGEVVEMGEEGGN--WSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGF 882
           FLDDGE + MG  GGN  W+LV+F     G  ++++S+V+N  +A   K  I KVT VGF
Sbjct: 790 FLDDGENLRMGAGGGNRDWTLVKFRCYVTGKSVVLRSEVVNPEYASKMKWSIGKVTFVGF 849

Query: 883 ERPKNMGGLGLDISKGANLDGNSGIRKTYEY-SAKFVNVEISGLSISIWEEFVMEL 904
           E  +N+    +  S+       S I+   +    +F++VE+S LS+ + ++F M L
Sbjct: 850 ENVENVKTYEVRTSERLRSPRISLIKTVSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of Cp4.1LG20g05920 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 758.1 bits (1956), Expect = 8.3e-219
Identity = 403/944 (42.69%), Postives = 572/944 (60.59%), Query Frame = 0

Query: 22  MATGSKSLQAFLRRRRLLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRV 81
           MA+ S SL   L       +++L L  CF              +    +G GYR+ S   
Sbjct: 1   MASSSSSLAFSL-------SLLLALILCF-----------SPTQSYKTIGKGYRLVSIEE 60

Query: 82  DPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPR 141
            P G      L + +K+ +YG D+  L +    ET  RLRV ITD+ ++RWEVP  ++PR
Sbjct: 61  SPDG-GFIGYLQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPR 120

Query: 142 RSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFS 201
               ++  +  +   SP T V     S+LIFS + T PF F+V RRS+ + LF+T+ +  
Sbjct: 121 EQPPQVGKVIGKSRKSPIT-VQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTTSS-- 180

Query: 202 DSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTR-KSFKLVPDKSKTLTLWNADIGSVNT 261
                LVFKDQY+++S+SLPK+ +SL+G+GE ++    KLVP  ++  TL+  D+ ++N 
Sbjct: 181 -----LVFKDQYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINL 240

Query: 262 DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLY 321
           + +LYG+HP Y+D+R+    GK  A   H VLLLNSNGMD+ Y GD ++YK IGG+ D Y
Sbjct: 241 NTDLYGSHPMYMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFY 300

Query: 322 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEV 381
           F AGPSP++V+DQYT+LIGRPAP+PYWS GFHQCR+GY N+S +E VV  Y KA IPL+V
Sbjct: 301 FIAGPSPLNVVDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDV 360

Query: 382 MWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRG 441
           +W D D+MDG+KDFT +P+ +P  K+  F+D +H+ G KY++I DPGI  N +YGT+ R 
Sbjct: 361 IWNDDDHMDGHKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRA 420

Query: 442 IEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEI 501
           + AD+FIKY+G P+L +VWPGPVYFPDFL+P +  +WGDEIK F D+VP DGLWIDMNE+
Sbjct: 421 MAADVFIKYEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEV 480

Query: 502 SNF------------------------ITSSTSSFSNLDNPPYKINNAGVQRPINNRTVP 561
           SNF                        +     + +  D+PPYKIN  GV  P+  +T+ 
Sbjct: 481 SNFCSGLCTIPEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIA 540

Query: 562 ASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDN 621
            S+ H+  + EY+ H++YGF E+ ATH  L+ V GKRPF+LSRSTFVGSG+Y AHWTGDN
Sbjct: 541 TSATHYNGVREYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDN 600

Query: 622 AATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTEELCR------------------ 681
             TW  L  +I ++LNFG+FG+PMVG+DICGF    TEELC                   
Sbjct: 601 QGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANY 660

Query: 682 ------------LAASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTY 741
                       +A SAR  L +RY +LP++YTL YEAH  G PIARPLFFSFP+    Y
Sbjct: 661 YSPRQELYQWDTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECY 720

Query: 742 EINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADH 801
             + QFLLG+  +ISPVL++G   V+A FP G+W+ +F+ +++V   +G+++TL AP + 
Sbjct: 721 GNSRQFLLGSSFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNF 780

Query: 802 INVHVREGNILALHGEATTTQAARKTAFKLLVVVSNGQS---SSGEVFLDDGEVVEMGEE 861
           +NVH+ +  IL        ++ AR T F L++    G S   ++G+++LD+ E+ EM   
Sbjct: 781 VNVHLYQNTILPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLG 840

Query: 862 GGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISK 902
            G  + V FY+      + + SQV  G FALS+  +I+KV+++G      +  + ++ S 
Sbjct: 841 NGQSTYVDFYASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSP 900

BLAST of Cp4.1LG20g05920 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 734.9 bits (1896), Expect = 7.5e-212
Identity = 397/905 (43.87%), Postives = 550/905 (60.77%), Query Frame = 0

Query: 38  LLSAVVLFLFTCFFRLTASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIEK 97
           LL A++L    CF  L  S           +A+G GYR+ S    P   S    L + + 
Sbjct: 7   LLVAIIL----CFSSLQCS-----------NAIGKGYRLISMEKSPDDGSFIGYLQVKQS 66

Query: 98  SSVYGPDVERLSIQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGS 157
           + +YG D+  L +  ++ T  RLRV ITD+ ++RWEVP  ++ R     +     +   S
Sbjct: 67  NKIYGSDITILRLFINYRTDHRLRVHITDAKKQRWEVPYNLLRREQPPNVIGKSRK---S 126

Query: 158 PET-SVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETF--LVFKDQYI 217
           P T   IS P   LIF++    PF F+V RRS+G+ +F+TS   S  E+F  +VFKDQY+
Sbjct: 127 PVTVQEISGPELILIFTVD---PFSFAVRRRSNGETIFNTS---SSDESFGEMVFKDQYL 186

Query: 218 QLSSSLPKDRSSLFGIGEQTR-KSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYID 277
           ++S+SLPKD +SL+G GE ++    KLVP  ++  TL+  D+ + N + +LYG+HP Y+D
Sbjct: 187 EISTSLPKD-ASLYGFGENSQANGIKLVP--NEPYTLFTEDVSAFNLNTDLYGSHPVYMD 246

Query: 278 VRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQ 337
           +R+ S  GK  A   H VLLLNS+GMD+ Y GD ++YK IGG+ D YFFAGPSP++V+DQ
Sbjct: 247 LRNVS--GKAYA---HSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQ 306

Query: 338 YTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKD 397
           YT LIGRPAP+PYWS GFHQCR+GY+NVS ++ VV  Y KA IPL+V+W D DYMDGYKD
Sbjct: 307 YTSLIGRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKD 366

Query: 398 FTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVP 457
           FT D +NFP  K+ +F+D +H+ G KYV+I DPGI  N +YG Y RG+ +D+FIKY+G P
Sbjct: 367 FTLDLVNFPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKP 426

Query: 458 YLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSN 517
           +L +VWPGPVYFPDFL+P +  +WGDEI+ F ++VP DGLWIDMNE              
Sbjct: 427 FLAQVWPGPVYFPDFLNPKTVSWWGDEIRRFHELVPIDGLWIDMNE-------------- 486

Query: 518 LDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKR 577
                  IN  G +  +  +T+P S+ H+  + EY+ H++YGF E+ ATH +L+ V GKR
Sbjct: 487 -------INATGHKASLGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKR 546

Query: 578 PFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTT 637
           PF+LSRSTFVGSG+Y AHWTGDN  TW  L  +I ++LNFG+FG+PMVG+DICGF   T 
Sbjct: 547 PFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTP 606

Query: 638 EELCR------------------------------LAASARKVLALRYSLLPYIYTLMYE 697
           EELC                               +A SAR  L +RY LLP++YTL YE
Sbjct: 607 EELCNRWIEVGAFYPFSRDHADYYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYE 666

Query: 698 AHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSL 757
           AH  G PIARPLFFSFP+    Y ++ QFLLG+ ++ISPVL++G   V+A FP G+W+ +
Sbjct: 667 AHMSGAPIARPLFFSFPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHM 726

Query: 758 FNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKLLVVVSNG 817
           F+ ++ V   +G+  TL AP + +NVH+ +  IL +         A           S G
Sbjct: 727 FDMTQVVVSKNGRLFTLPAPFNVVNVHLYQNAILPMQQVVAFPAGA-----------SEG 786

Query: 818 QSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKV 877
             +SG++FLDD E+ EM    G  + + FY+      + I SQV  G FALSQ ++I+KV
Sbjct: 787 Y-ASGKLFLDDDELPEMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKV 846

Query: 878 TIVGFERPKNMGGL---GLDISKGANLDGNSGIRKTY------EYSAKFVNVEISGLSIS 900
            ++G +    +  +   G  IS        S   + Y      E  +K   VE+ GL + 
Sbjct: 847 IVLGLKGTWKVSEILLNGSSISNETKTIEVSSKEQMYVVGSEDEGESKSFMVELKGLEML 846

BLAST of Cp4.1LG20g05920 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 279.6 bits (714), Expect = 8.7e-75
Identity = 210/690 (30.43%), Postives = 315/690 (45.65%), Query Frame = 0

Query: 223 DRSSLFGIGEQTRKSFKLVPDK------SKTLTLWNADIGSVN--TDVNLYGAHPFYIDV 282
           D S ++GI E    SF L P K      S+   L+N D+   +  +   LYG+ PF +  
Sbjct: 222 DSSFVYGIPEHA-TSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVS- 281

Query: 283 RSPSRDGKVAAGTTHGVLLLNS---------------NGMDIIYSGDRIS--YKAIGGII 342
                 GK  +G T G   LN+               +G+ +  S  RI   + +  GI+
Sbjct: 282 -----HGK--SGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 343 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIP 402
           D +FF GP P  V+ QY  + G  A    ++ G+HQCR+ YK+  D+  V +++ +  IP
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIP 401

Query: 403 LEVMWTDIDYMDGYKDFTFDPINFP-VEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGT 462
            +V+W DI++ DG + FT+D + FP  E+M+     L   G+K V I+DP I  + +Y  
Sbjct: 402 YDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQK---KLAAKGRKMVTIVDPHIKRDDSYFL 461

Query: 463 YIRGIEADIFIK-YDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEI--KLFRDIVPFDGL 522
           +    +   ++K   G  + G  WPG   + D L P    +WG     K +    P    
Sbjct: 462 HKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYT 521

Query: 523 WIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNL 582
           W DMNE S F            N P               T+P  +LH G +     HN 
Sbjct: 522 WNDMNEPSVF------------NGP-------------EVTMPRDALHVGGVEHREVHNA 581

Query: 583 YGFLESKATHASLV-KVTGK-RPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSIL 642
           YG+    AT   LV +  GK RPFVLSR+ F G+ +Y A WTGDN A W  L  +IP IL
Sbjct: 582 YGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMIL 641

Query: 643 NFGLFGIPMVGADICGFSRDTTEELC-------------------------------RLA 702
             GL GI   GADI GF  +   EL                                R  
Sbjct: 642 TLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNT 701

Query: 703 ASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLI 762
              R  +  RY+LLPY YTL  EA+  G P+ RPL+  FPQD  T+  +  F++G+G+L+
Sbjct: 702 ELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLLV 761

Query: 763 SPVLKEGAISVDAYFPA-GNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILAL 822
             V  +G      Y P   +W+ L N    V    G+   +DAP + I    + G I+  
Sbjct: 762 QGVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGTIIPR 821

Query: 823 HGE-ATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRF-YSEAV 848
                 ++       + L+V +++ Q + GE+++DDG+  E     G++   RF +S+ V
Sbjct: 822 KDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEF--RRGSYIHRRFVFSKGV 865

BLAST of Cp4.1LG20g05920 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 270.0 bits (689), Expect = 6.9e-72
Identity = 196/654 (29.97%), Postives = 300/654 (45.87%), Query Frame = 0

Query: 225 SSLFGIGEQTRKSFKLVPDKSKTLTLWNADI-GSVNTDVNLYGAHPFYIDVRSPSRDGKV 284
           +S +G GE + +    +    K +  WN D  G  +   +LY +HP+ + V        V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 285 AAGTTHGVLL-LNSNGMDIIYSGDRISYKAIGGIIDLYFFAGPSPISVIDQYTELIGRPA 344
            A TT    + L   G+  I S    SY  I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 345 PVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFP 404
             P W+ G+HQCR+ Y +   +  +   +    IP +V+W DIDYMDG++ FTFD   FP
Sbjct: 206 MPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFP 265

Query: 405 VEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFI-KYDGVPYLGEVWPG 464
                    +LH NG K + +LDPGI   + Y  Y  G + D++I + DG P+ GEVWPG
Sbjct: 266 --DPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPG 325

Query: 465 PVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNPPYKI 524
           P  FPD+ +  +  +W + +K F      DG+W DMNE + F                  
Sbjct: 326 PCVFPDYTNSKARSWWANLVKEFVS-NGVDGIWNDMNEPAVF------------------ 385

Query: 525 NNAGVQRPINNRTVPASSLHFGN------LTEYNTHNLYGFLESKATHASL-VKVTGKRP 584
                   +  +T+P +++H G+          + HN+YG L +++T+  + +    KRP
Sbjct: 386 -------KVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRP 445

Query: 585 FVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSRDTTE 644
           FVL+R+ F+GS +Y A WTGDN + W  L  +I  +L  GL G P+ G DI GF+ + T 
Sbjct: 446 FVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATP 505

Query: 645 EL-------------CRLAASA------------------RKVLALRYSLLPYIYTLMYE 704
            L             CR  + A                  R  L  RY LLP+ YTL Y 
Sbjct: 506 RLFGRWMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYI 565

Query: 705 AHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVL-KEGAISVDAYFPAGNWFS 764
           AH  G P+A P+FF+ P D R   + + FLLG  ++ +  L  +G+  +    P G W  
Sbjct: 566 AHTTGAPVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHR 625

Query: 765 LFNYSES------VAVNSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTAFKL 824
            F++++S      + +  G  I+L  P    ++HV E    +L  + T           L
Sbjct: 626 -FDFADSHPDLPTLYLQGGSIISLAPP----HLHVGE---FSLSDDLT-----------L 678

Query: 825 LVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLIKSQVING 831
           LV +     + G +F DDG+    G   G + +  + +E   S + +K     G
Sbjct: 686 LVSLDENGKAKGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678