Cp4.1LG20g05490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g05490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionphospholipase A1-Igamma1, chloroplastic-like
LocationCp4.1LG20: 3203069 .. 3204661 (-)
RNA-Seq ExpressionCp4.1LG20g05490
SyntenyCp4.1LG20g05490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGGAGAATCGTGACGCCGAGAGGAGTCCCACTGAAAAGAGTAACGATGTTAAGAAACGGAACGAAGAAGACGAAATGGTATTGGAAATTGAAATGGGGGATCCGATTAAGAGCCATAAAAAATGCTCTCTCTTCTTCCTTACACAACCAGCGCCGCCGTTTGACATGTGCTACCGCCACCGGCCAACTAACTCCCGTCAACGGTGCAGCGTCGCCGCTTGTAATTCAGCCATCGAAATCAACCTCTAAGAAAAACGACCTCCGTTTGGCAAAGCCACTCGCCTCGCTCCTCCGTATGCCACAACGCGCCGCTGACTTCGTCGACTACGGCAACCACATGACCCCAACATTCTCCCCTCGTGATAAAATCGCCGATGTATGGCGTGACCTCCACGGCGGTTCTAATTGGGAAGGTCTACTAGATCCCCTCCACCCCTTCCTCCGCCGCGAGCTTGTTAAATACGGCGAGTTCGCTCAAGCCACCTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCCGCGGCAGCTGTAGATACAACCGCCATAAGCTCCTCCACGAATTGGGTCTGGCTCAAAACGGCTACAAAGTGACTAGATACATCTACGCCTTGTCCCCTGTTGACGGTCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTGTGGAGCAGAGATTCGAATTGGATGGGGTTTGTAGCCGTTAGTAACGACGAGGAGAGCGAGAGAATCGGGCGGCGGGATATCCTCGTGGCCTGGAGAGGAACGGTAACCCCAACGGAATGGTACATCGATCTGAAAACCAAGCTGAAAAAAATCGACCGGAGCGACAGGAAAATCAAAGTCCAACGTGGGTTCTTAACAATTTACAAATCGAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCGTCAGAGCAGGTAATGGAGGAGCTACATAGACTAATCGATTTCTTCACACAAAAGGGCGACAGAGAAATCAGCCTAACAATTGTCGGACACAGCCTAGGCGGCGCGCTGTCGCTTCTGACAGCCTACGAGGCAGGGGCATCATTTCCCGACATCCACGTGAGCGTAATGTCATTCGGAGCGCCAAGAGTGGGGAATTTAGCATTCAGGGAGAAATTAAACGAGATGGGTGTGAAGACACTCCGCGTAGTGATACGACAGGACATTGTCCCCAAGCTTCCGGGGCTGTTCGTGAATTCGATTGTGAACAAGCTGAGCGCAGTGACAGGGAAGCTGAATTGGCTGTACAGACATGTGGGGACGCAGCTGAAAATGGACATGTACATGTCGCCGTACTTGAAGAGGGAGTCGGACATGTCGGGGAGCCATAATTTGGAGATATATTTGCATTTGGTGGATGGGTTTGTGAGTAGGAGGGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAACAAAGGGAGCGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAGGGATTGGTGAAGAACAGGTTTGGGAGATGGGTTAAGCCTGGGAGGAACGCTGAGGATATTCCTTCTCCCTTCTCTCGTCCTTCCAATATTTGA

mRNA sequence

ATGGAGGGGAGAATCGTGACGCCGAGAGGAGTCCCACTGAAAAGAGTAACGATGTTAAGAAACGGAACGAAGAAGACGAAATGGTATTGGAAATTGAAATGGGGGATCCGATTAAGAGCCATAAAAAATGCTCTCTCTTCTTCCTTACACAACCAGCGCCGCCGTTTGACATGTGCTACCGCCACCGGCCAACTAACTCCCGTCAACGGTGCAGCGTCGCCGCTTGTAATTCAGCCATCGAAATCAACCTCTAAGAAAAACGACCTCCGTTTGGCAAAGCCACTCGCCTCGCTCCTCCGTATGCCACAACGCGCCGCTGACTTCGTCGACTACGGCAACCACATGACCCCAACATTCTCCCCTCGTGATAAAATCGCCGATGTATGGCGTGACCTCCACGGCGGTTCTAATTGGGAAGGTCTACTAGATCCCCTCCACCCCTTCCTCCGCCGCGAGCTTGTTAAATACGGCGAGTTCGCTCAAGCCACCTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCCGCGGCAGCTGTAGATACAACCGCCATAAGCTCCTCCACGAATTGGGTCTGGCTCAAAACGGCTACAAAGTGACTAGATACATCTACGCCTTGTCCCCTGTTGACGGTCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTGTGGAGCAGAGATTCGAATTGGATGGGGTTTGTAGCCGTTAGTAACGACGAGGAGAGCGAGAGAATCGGGCGGCGGGATATCCTCGTGGCCTGGAGAGGAACGGTAACCCCAACGGAATGGTACATCGATCTGAAAACCAAGCTGAAAAAAATCGACCGGAGCGACAGGAAAATCAAAGTCCAACGTGGGTTCTTAACAATTTACAAATCGAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCGTCAGAGCAGGTAATGGAGGAGCTACATAGACTAATCGATTTCTTCACACAAAAGGGCGACAGAGAAATCAGCCTAACAATTGTCGGACACAGCCTAGGCGGCGCGCTGTCGCTTCTGACAGCCTACGAGGCAGGGGCATCATTTCCCGACATCCACGTGAGCGTAATGTCATTCGGAGCGCCAAGAGTGGGGAATTTAGCATTCAGGGAGAAATTAAACGAGATGGGTGTGAAGACACTCCGCGTAGTGATACGACAGGACATTGTCCCCAAGCTTCCGGGGCTGTTCGTGAATTCGATTGTGAACAAGCTGAGCGCAGTGACAGGGAAGCTGAATTGGCTGTACAGACATGTGGGGACGCAGCTGAAAATGGACATGTACATGTCGCCGTACTTGAAGAGGGAGTCGGACATGTCGGGGAGCCATAATTTGGAGATATATTTGCATTTGGTGGATGGGTTTGTGAGTAGGAGGGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAACAAAGGGAGCGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAGGGATTGGTGAAGAACAGGTTTGGGAGATGGGTTAAGCCTGGGAGGAACGCTGAGGATATTCCTTCTCCCTTCTCTCGTCCTTCCAATATTTGA

Coding sequence (CDS)

ATGGAGGGGAGAATCGTGACGCCGAGAGGAGTCCCACTGAAAAGAGTAACGATGTTAAGAAACGGAACGAAGAAGACGAAATGGTATTGGAAATTGAAATGGGGGATCCGATTAAGAGCCATAAAAAATGCTCTCTCTTCTTCCTTACACAACCAGCGCCGCCGTTTGACATGTGCTACCGCCACCGGCCAACTAACTCCCGTCAACGGTGCAGCGTCGCCGCTTGTAATTCAGCCATCGAAATCAACCTCTAAGAAAAACGACCTCCGTTTGGCAAAGCCACTCGCCTCGCTCCTCCGTATGCCACAACGCGCCGCTGACTTCGTCGACTACGGCAACCACATGACCCCAACATTCTCCCCTCGTGATAAAATCGCCGATGTATGGCGTGACCTCCACGGCGGTTCTAATTGGGAAGGTCTACTAGATCCCCTCCACCCCTTCCTCCGCCGCGAGCTTGTTAAATACGGCGAGTTCGCTCAAGCCACCTACGACGCTTTTGACTTCGACCCACTTTCTGAATTCCGCGGCAGCTGTAGATACAACCGCCATAAGCTCCTCCACGAATTGGGTCTGGCTCAAAACGGCTACAAAGTGACTAGATACATCTACGCCTTGTCCCCTGTTGACGGTCCGGATTGGTTCGAGAGTTCCAAAATCGGCGAGGTGTGGAGCAGAGATTCGAATTGGATGGGGTTTGTAGCCGTTAGTAACGACGAGGAGAGCGAGAGAATCGGGCGGCGGGATATCCTCGTGGCCTGGAGAGGAACGGTAACCCCAACGGAATGGTACATCGATCTGAAAACCAAGCTGAAAAAAATCGACCGGAGCGACAGGAAAATCAAAGTCCAACGTGGGTTCTTAACAATTTACAAATCGAAAGACGAGGAATCAAAATTCAACAAAACCAGCGCGTCAGAGCAGGTAATGGAGGAGCTACATAGACTAATCGATTTCTTCACACAAAAGGGCGACAGAGAAATCAGCCTAACAATTGTCGGACACAGCCTAGGCGGCGCGCTGTCGCTTCTGACAGCCTACGAGGCAGGGGCATCATTTCCCGACATCCACGTGAGCGTAATGTCATTCGGAGCGCCAAGAGTGGGGAATTTAGCATTCAGGGAGAAATTAAACGAGATGGGTGTGAAGACACTCCGCGTAGTGATACGACAGGACATTGTCCCCAAGCTTCCGGGGCTGTTCGTGAATTCGATTGTGAACAAGCTGAGCGCAGTGACAGGGAAGCTGAATTGGCTGTACAGACATGTGGGGACGCAGCTGAAAATGGACATGTACATGTCGCCGTACTTGAAGAGGGAGTCGGACATGTCGGGGAGCCATAATTTGGAGATATATTTGCATTTGGTGGATGGGTTTGTGAGTAGGAGGGGGAAGTTCAGGTGGAATTCGAGGAGGGATGTGGCGTTGGTGAACAAAGGGAGCGATATGCTTGTGGAAGAGCTGAGGATTCCGGAGTTTTGGTATCAGTTGCCGCATAAGGGATTGGTGAAGAACAGGTTTGGGAGATGGGTTAAGCCTGGGAGGAACGCTGAGGATATTCCTTCTCCCTTCTCTCGTCCTTCCAATATTTGA

Protein sequence

MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
Homology
BLAST of Cp4.1LG20g05490 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 392.5 bits (1007), Expect = 7.6e-108
Identity = 197/415 (47.47%), Postives = 274/415 (66.02%), Query Frame = 0

Query: 124 KIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNR 183
           ++ D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S++ G+ R+ R
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 184 HKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 243
            +    LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+DE S 
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 244 ERIGRRDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDRKIKVQRGFLTIYKSKDEE 303
            R+GRRDI +AWRGTVT  EW  DLK  LK     KI   D  +KV+ GFL +Y  KD  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTT 279

Query: 304 SKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD-- 363
            KF + SA EQ++ E+ RL++      D ++S+T+ GHSLGGAL++L+AY+      +  
Sbjct: 280 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 339

Query: 364 -----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVN-SIVNKL 423
                I V+V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L
Sbjct: 340 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 399

Query: 424 SAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWN 483
             +   L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +
Sbjct: 400 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLS 459

Query: 484 SRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
           S RD ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 460 SGRDHALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Cp4.1LG20g05490 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 2.9e-107
Identity = 201/474 (42.41%), Postives = 289/474 (60.97%), Query Frame = 0

Query: 73  SPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRD-------KI 132
           S L+  P++S+  +   R  +  +   R+ Q +     YG         RD       ++
Sbjct: 31  STLITFPARSSPARAMSRTDEEASISTRLEQES-----YGLTTAEDIRRRDGEAKESKRL 90

Query: 133 ADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHK 192
            D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S + GSCR+ R  
Sbjct: 91  RDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRH 150

Query: 193 LLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESE-- 252
           L   LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+D E+   
Sbjct: 151 LFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRC 210

Query: 253 RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS-----DRKIKVQRGFLTIYKSKDEES 312
           R+GRRDI +AWRGTVT  EW  DLK  LK +  +     D  +K + GFL +Y  KD   
Sbjct: 211 RLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSC 270

Query: 313 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD--- 372
            F+K SA EQV+ E+ RL++ +  +   E+S+T+ GHSLGGAL++L+AY+      +   
Sbjct: 271 NFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTR 330

Query: 373 ----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNS-IVNKLS 432
               I V+  ++G PRVGN+ F+E++ ++GVK LRVV   D+V K PGLF+N      L 
Sbjct: 331 KGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 390

Query: 433 AVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNS 492
            + G L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +S
Sbjct: 391 KLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSS 450

Query: 493 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
            RD ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R  A+D  +P
Sbjct: 451 GRDPALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAP 499

BLAST of Cp4.1LG20g05490 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.9e-98
Identity = 185/409 (45.23%), Postives = 263/409 (64.30%), Query Frame = 0

Query: 125 IADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRH 184
           + ++WR++ G +NWEG LDP++  LRRE+++YGEFAQA YD+FDFDP S++ GSC+Y+  
Sbjct: 87  LREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPS 146

Query: 185 KLLHELGL-AQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 244
                L L    GY +TRY+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+ DEE  
Sbjct: 147 DFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEV 206

Query: 245 ERIGRRDILVAWRGTVTPTEWYIDLKTKLKKID-RSDRKIKVQRGFLTIYKSKDEESKFN 304
            R+GRRDI++AWRGTVT  EW  DLK  L   +   D  IK++ GF  +Y  K++  KF+
Sbjct: 207 SRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFS 266

Query: 305 KTSASEQVMEELHRLIDFF-TQKGDREISLTIVGHSLGGALSLLTAYEAGA----SFPD- 364
             SA EQV+ E+ RLI+++ T++   + S+T+ GHSLG +L+L++AY+         P+ 
Sbjct: 267 SFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPEN 326

Query: 365 ---IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 424
              I ++V SF  PRVGNL F+E+ +E+GVK LRVV   D VP +PG+F N        V
Sbjct: 327 NYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYV 386

Query: 425 TGKLN--WLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSR----RGKF 484
             K +  W Y HVG +L +D   SP+LK   D+  +HNLE  LHLVDG+  +      +F
Sbjct: 387 EEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRF 446

Query: 485 RWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR 516
              ++RD+ALVNK  D L  E  +P  W Q  +KG+VKN  G+WV P R
Sbjct: 447 CLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDR 495

BLAST of Cp4.1LG20g05490 vs. ExPASy Swiss-Prot
Match: B9EYD3 (Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 PE=2 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 2.8e-70
Identity = 163/409 (39.85%), Postives = 228/409 (55.75%), Query Frame = 0

Query: 118 TFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRG 177
           T +PR  +A+ WR+LHG  +W+GLLDPL   LRR ++ YGE AQAT DAF  +  S   G
Sbjct: 3   TTAPR-AVAERWRELHGEDHWKGLLDPLDADLRRSVIGYGELAQATNDAFIREAWSPHAG 62

Query: 178 SCRYNRHKLLHELGLA---QNGYKVTRYIYALSPVDG-PDWFESSKIGEVWSRDSNWMGF 237
           +CRY+R + L +   +      Y+VT + YA +   G P  F       V +R+SNWMG+
Sbjct: 63  ACRYSRDRFLEKAQASTQLAGLYEVTAFFYATAGAGGVPAPF------MVRNRESNWMGY 122

Query: 238 VAVSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKI-------DRSDRKIKVQRG 297
           VAV+ D     +GRRD++VAWRGTV P EW  DL   L           RS    +V RG
Sbjct: 123 VAVATDAGVAALGRRDVVVAWRGTVRPMEWLNDLDFTLVSAAGVLGAGGRSPAP-RVHRG 182

Query: 298 FLTIYKSKDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTA 357
           +L+IY + D  SK++K SA EQ+ +E+ RL+D +    D E S+T+VGHSLG A++ L A
Sbjct: 183 WLSIYTASDPASKYSKLSAREQISDEIKRLMDKYK---DEETSITVVGHSLGAAVATLNA 242

Query: 358 YEAGASFPDIH----VSVMSFGAPRVGNLAFREKLNEM-GVKTLRVVIRQDIVPKLPGLF 417
            +  ++  + H    V+ ++F  PRVG+  FR+  +E+ G++ LRV    D+VPK P + 
Sbjct: 243 ADIVSNGLNQHGACPVTAVAFACPRVGDSGFRKLFDELPGLRLLRVCNSPDVVPKYPPMG 302

Query: 418 VNSIVNKLSAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVS 477
                             Y  VG +L +D   SPYLK   + +  H+LE Y+H V G   
Sbjct: 303 ------------------YADVGVELPVDTRRSPYLKSPGNQAVWHSLECYMHGVAGAQG 362

Query: 478 RRGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRW 511
           +RG F+    RDVALVNK  D L EE  +P  W     KG+V+   G W
Sbjct: 363 KRGGFKLEVDRDVALVNKNVDALKEEYHVPPSWSVQRDKGMVRGADGHW 382

BLAST of Cp4.1LG20g05490 vs. ExPASy Swiss-Prot
Match: O23522 (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 1.8e-69
Identity = 173/494 (35.02%), Postives = 256/494 (51.82%), Query Frame = 0

Query: 50  HNQRRRLTCATATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFV 109
           H +RRR TC T T  L P      PL + P++ T+K++     + L ++LR    + D +
Sbjct: 12  HAKRRRFTCNTHTSTLIP----TKPLSVSPARKTNKEH----LRNLENVLRTSSNSIDHI 71

Query: 110 D---------------------------------YGNHMTP---------------TFSP 169
           +                                   + M+P                 SP
Sbjct: 72  ENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEERSP 131

Query: 170 RDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRY 229
           + K+   WR+LHG +NW GLLDPL   LRRELV+YGEF QA Y AF  DP     GS R+
Sbjct: 132 KSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDP----EGSPRH 191

Query: 230 NRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVW-SRDSNWMGFVAVSND- 289
                   + L    +KVT+ +YA S V  P W +       W ++ ++W+G+VAV +D 
Sbjct: 192 --------VALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDP 251

Query: 290 EESERIGRRDILVAWRGTVTPTEWYIDLKTKL-----KKIDRSD-RKIKVQRGFLTIYKS 349
            E  R+GRR+I++A RGT T  EW  + +  L      K D+SD  + KV+ GF ++Y +
Sbjct: 252 REIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTT 311

Query: 350 KDEESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASF 409
            D+ +     S +E ++ E+ RL++ +      E+S+++ GHSLG A++LL A +     
Sbjct: 312 GDQHA----PSLAESLVGEISRLVELY---AGEELSISVTGHSLGAAIALLAADDIAERV 371

Query: 410 PDI-HVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 469
           P    V+V SFG PRVGN  F ++L+  GVK LRVV  QD+V K+PG+F ++     S  
Sbjct: 372 PHAPPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRN 431

Query: 470 TGKL------------NWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFV 475
            G+              W Y HVG +L++DM MSPYLK  +D++  H+LE YLHLVDGF+
Sbjct: 432 NGRSPGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFL 478

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: XP_023519232.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: KAG7019492.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 527/530 (99.43%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           +SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 ISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLK+ESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: KAG6583869.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 526/530 (99.25%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNAL+SSLHNQRRRLTCAT
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALASSLHNQRRRLTCAT 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           +SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 ISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLK+ESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: XP_022927629.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1071 bits (2769), Expect = 0.0
Identity = 526/530 (99.25%), Postives = 529/530 (99.81%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGA SPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           +SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 ISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLK+ESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Cp4.1LG20g05490 vs. NCBI nr
Match: XP_023001410.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 519/530 (97.92%), Postives = 526/530 (99.25%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCST 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGAASPLVIQPSKST+KKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHG SNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSE+RGSCR
Sbjct: 121 PRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEYRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS+DE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSDDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD+KIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLI FFTQKGDREISLTIVGHSLGGALSLLTAYEAG SFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVSFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           +SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 ISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPF RPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Cp4.1LG20g05490 vs. ExPASy TrEMBL
Match: A0A6J1EIJ3 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434398 PE=4 SV=1)

HSP 1 Score: 1071 bits (2769), Expect = 0.0
Identity = 526/530 (99.25%), Postives = 529/530 (99.81%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGA SPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAVSPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR
Sbjct: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           +SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 ISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLK+ESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of Cp4.1LG20g05490 vs. ExPASy TrEMBL
Match: A0A6J1KL46 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495553 PE=4 SV=1)

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 519/530 (97.92%), Postives = 526/530 (99.25%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTC+T
Sbjct: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCST 60

Query: 61  ATGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120
           ATGQLTPVNGAASPLVIQPSKST+KKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS
Sbjct: 61  ATGQLTPVNGAASPLVIQPSKSTAKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFS 120

Query: 121 PRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCR 180
           PRDKIADVWRDLHG SNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSE+RGSCR
Sbjct: 121 PRDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEYRGSCR 180

Query: 181 YNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDE 240
           YNRHKLLHELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS+DE
Sbjct: 181 YNRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSDDE 240

Query: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDEESKF 300
           ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD+KIKVQRGFLTIYKSKDEESKF
Sbjct: 241 ESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDKKIKVQRGFLTIYKSKDEESKF 300

Query: 301 NKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHVSV 360
           NKTSASEQVMEELHRLI FFTQKGDREISLTIVGHSLGGALSLLTAYEAG SFPDIHVSV
Sbjct: 301 NKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVSFPDIHVSV 360

Query: 361 MSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420
           +SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY
Sbjct: 361 ISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLY 420

Query: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480
           RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG
Sbjct: 421 RHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKG 480

Query: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPF RPSNI
Sbjct: 481 SDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of Cp4.1LG20g05490 vs. ExPASy TrEMBL
Match: A0A5A7UFJ9 (Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001160 PE=4 SV=1)

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 467/536 (87.13%), Postives = 502/536 (93.66%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           ME ++ TPRG+P+KR TMLRNGTKKTKWYWKLK GIR RAIK ALSSSLHNQRRRLTC+T
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 61  A-----TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHM 120
           A     T QLTP NG  SPLVI  SK  SKKNDLRLAKPLASLLRMP RAADF+D+GNHM
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSKP-SKKNDLRLAKPLASLLRMPLRAADFIDFGNHM 120

Query: 121 TPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF 180
           TPT SPRD I+ VWRDLHG S+WEGLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF
Sbjct: 121 TPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 180

Query: 181 RGSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVA 240
            GSCRYNRHKLL+ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VA
Sbjct: 181 CGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVA 240

Query: 241 VSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKD 300
           VS+DEES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS++K+KVQRGFLTIYKSKD
Sbjct: 241 VSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKD 300

Query: 301 EESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP- 360
           E+SKFNKTSASEQVMEELHRLIDFF +KGDREISLTI GHSLGGALSLLTAYEAG +FP 
Sbjct: 301 EDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPA 360

Query: 361 DIHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTG 420
           D+HVSV+SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKL AVTG
Sbjct: 361 DVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTG 420

Query: 421 KLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDV 480
           KLNW+YRHVG +L+M+MYMSPYLK++SDMSGSHNLEIYLHLVDGFV+++GKFRWNSRRDV
Sbjct: 421 KLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDV 480

Query: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           ALVNKGSDMLVEELRIPEFWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+P  +
Sbjct: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Cp4.1LG20g05490 vs. ExPASy TrEMBL
Match: A0A1S3BBX3 (phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 PE=4 SV=1)

HSP 1 Score: 957 bits (2473), Expect = 0.0
Identity = 467/536 (87.13%), Postives = 502/536 (93.66%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           ME ++ TPRG+P+KR TMLRNGTKKTKWYWKLK GIR RAIK ALSSSLHNQRRRLTC+T
Sbjct: 1   MEAKLATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLTCST 60

Query: 61  A-----TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHM 120
           A     T QLTP NG  SPLVI  SK  SKKNDLRLAKPLASLLRMP RAADF+D+GNHM
Sbjct: 61  AITTTTTPQLTPTNGLVSPLVIHQSKP-SKKNDLRLAKPLASLLRMPLRAADFIDFGNHM 120

Query: 121 TPTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEF 180
           TPT SPRD I+ VWRDLHG S+WEGLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF
Sbjct: 121 TPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEF 180

Query: 181 RGSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVA 240
            GSCRYNRHKLL+ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMG+VA
Sbjct: 181 CGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGYVA 240

Query: 241 VSNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKD 300
           VS+DEES+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS++K+KVQRGFLTIYKSKD
Sbjct: 241 VSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKD 300

Query: 301 EESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP- 360
           E+SKFNKTSASEQVMEELHRLIDFF +KGDREISLTI GHSLGGALSLLTAYEAG +FP 
Sbjct: 301 EDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTAYEAGVTFPA 360

Query: 361 DIHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTG 420
           D+HVSV+SFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKL AVTG
Sbjct: 361 DVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLGAVTG 420

Query: 421 KLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDV 480
           KLNW+YRHVG +L+M+MYMSPYLK++SDMSGSHNLEIYLHLVDGFV+++GKFRWNSRRDV
Sbjct: 421 KLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGKFRWNSRRDV 480

Query: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           ALVNKGSDMLVEELRIPEFWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+P  +
Sbjct: 481 ALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQPLQV 535

BLAST of Cp4.1LG20g05490 vs. ExPASy TrEMBL
Match: A0A0A0M111 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE=4 SV=1)

HSP 1 Score: 949 bits (2452), Expect = 0.0
Identity = 464/535 (86.73%), Postives = 501/535 (93.64%), Query Frame = 0

Query: 1   MEGRIVTPRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCAT 60
           ME +  TPRG+P+KR TMLRNGTKKTKWYWKLK GI+ +AIK ALSSSLHNQRRRLTC+T
Sbjct: 1   MEPKFATPRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCST 60

Query: 61  A----TGQLTPVNGAASPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMT 120
           A    T Q TP NG  SPLVI  SKS SKKNDLRLAKPLASLLRMP RA+DF+D+GNHMT
Sbjct: 61  AITTTTPQHTPTNGLVSPLVIHQSKS-SKKNDLRLAKPLASLLRMPLRASDFIDFGNHMT 120

Query: 121 PTFSPRDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFR 180
           PT SPRDKI+ VWRDLHG S+WEGLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLSEF 
Sbjct: 121 PTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSEFC 180

Query: 181 GSCRYNRHKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240
           GSCRYNR KLL+ELGLAQNGYKVT+YIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV
Sbjct: 181 GSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAV 240

Query: 241 SNDEESERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSDRKIKVQRGFLTIYKSKDE 300
           S+DEES+RIGRRDILV+WRGTVTPTEWYIDLKTKLKKIDRS++K+KVQRGFLTIYKSKDE
Sbjct: 241 SSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDRSNKKVKVQRGFLTIYKSKDE 300

Query: 301 ESKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFP-D 360
           +SKFNKTSASEQVMEELHRLIDFF QKGDREISLTI GHSLGGALSLLTAYEAG +FP D
Sbjct: 301 DSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGVTFPAD 360

Query: 361 IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGK 420
           +HVSV+SFGAPRVGNLAFREKLNEMGVK LRVVI QDIVPKLPGLFVNSIVNKLSAVTGK
Sbjct: 361 VHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLSAVTGK 420

Query: 421 LNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVA 480
           LNW+YRHVG +L+M+M+MSPYLK++SDMSGSHNLEIYLHLVDGFV+++GKFRWNSRRDVA
Sbjct: 421 LNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNSRRDVA 480

Query: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530
           LVNKGSDMLVEELRIPEFWYQLPHKGL+KNRFGRWVKPGRN EDIPSPFS+PS +
Sbjct: 481 LVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of Cp4.1LG20g05490 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 585.5 bits (1508), Expect = 4.3e-167
Identity = 291/513 (56.73%), Postives = 385/513 (75.05%), Query Frame = 0

Query: 24  KKTKWYWKLKWGIRL--RAIKNALSSSLH---NQRRRLTCATATGQLTPVNGAASPLVIQ 83
           ++TK  W+LK  ++L  ++IK  + S L    + ++ L    +  +   ++  A  +   
Sbjct: 16  RRTKRVWRLKQKLKLAWKSIKIRVKSHLPGFLSTKKHLFHIKSRKEEQDLSQVAQRICKI 75

Query: 84  PSKSTSKKNDLRLAKPLASLLRMPQRAA-DFVDYGNHMTPTFSPRDKIADVWRDLHGGSN 143
            + ST         K LA LL++P+ +A DF+D G+ MTP  SPR+KI+ +WR+LHG +N
Sbjct: 76  SNDST---------KSLAFLLQLPKYSADDFLDRGDLMTPAASPREKISKMWRELHGSNN 135

Query: 144 WEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHKLLHELGLAQNGY 203
           WE LLDPLHP+LRRE+ KYGEF ++ YD+ DFDPLSEF GS RYNR+KL  ELGL ++GY
Sbjct: 136 WENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRNKLFEELGLTRHGY 195

Query: 204 KVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESERIGRRDILVAWRGT 263
           KVT+YIYA+S VD P WF SS +GE WS+DSNWMGFVAVS D ES RIGRRDI+VAWRGT
Sbjct: 196 KVTKYIYAMSRVDVPQWFLSSALGETWSKDSNWMGFVAVSGDRESLRIGRRDIVVAWRGT 255

Query: 264 VTPTEWYIDLKTKLKKIDRSDRK----IKVQRGFLTIYKSKDEESKFNKTSASEQVMEEL 323
           VTPTEW++DL+T ++  D   +     +KVQ GFL+IY SK E +++NK SASEQ M+E+
Sbjct: 256 VTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSIYNSKSELTRYNKESASEQTMDEV 315

Query: 324 HRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDI--HVSVMSFGAPRVGNL 383
            RL++FF  +G+ E+SLTI GHSLGGAL+L+ AYEA    P +  ++SV+SFGAPRVGNL
Sbjct: 316 KRLVNFFKDRGE-EVSLTITGHSLGGALALMNAYEAARDVPALSGNISVISFGAPRVGNL 375

Query: 384 AFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNWLYRHVGTQLKMDM 443
           AF+EKLN +GVK LRVV +QDIVPKLPG+  N ++NKL+ +T +LNW+YRHVGTQLK+D+
Sbjct: 376 AFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNKLNPITSRLNWVYRHVGTQLKLDV 435

Query: 444 YMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVNKGSDMLVEELRIP 503
           + SPY+KR+SD+  +HNLE+YLH++DGF  ++  FR N+RRDVA VNK +DML++ LRIP
Sbjct: 436 FSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRVNARRDVASVNKSTDMLLDHLRIP 495

Query: 504 EFWYQLPHKGLVKNR-FGRWVKPGRNAEDIPSP 524
           EFWYQ+ HKGL+ N+  GRWVKP R  EDIPSP
Sbjct: 496 EFWYQVAHKGLILNKQTGRWVKPVRAPEDIPSP 518

BLAST of Cp4.1LG20g05490 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 392.5 bits (1007), Expect = 5.4e-109
Identity = 197/415 (47.47%), Postives = 274/415 (66.02%), Query Frame = 0

Query: 124 KIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNR 183
           ++ D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S++ G+ R+ R
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 184 HKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 243
            +    LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+DE S 
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 244 ERIGRRDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDRKIKVQRGFLTIYKSKDEE 303
            R+GRRDI +AWRGTVT  EW  DLK  LK     KI   D  +KV+ GFL +Y  KD  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTT 279

Query: 304 SKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD-- 363
            KF + SA EQ++ E+ RL++      D ++S+T+ GHSLGGAL++L+AY+      +  
Sbjct: 280 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 339

Query: 364 -----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVN-SIVNKL 423
                I V+V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L
Sbjct: 340 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 399

Query: 424 SAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWN 483
             +   L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +
Sbjct: 400 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYHGKGERFVLS 459

Query: 484 SRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
           S RD ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 460 SGRDHALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of Cp4.1LG20g05490 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 390.6 bits (1002), Expect = 2.0e-108
Identity = 201/474 (42.41%), Postives = 289/474 (60.97%), Query Frame = 0

Query: 73  SPLVIQPSKSTSKKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSPRD-------KI 132
           S L+  P++S+  +   R  +  +   R+ Q +     YG         RD       ++
Sbjct: 31  STLITFPARSSPARAMSRTDEEASISTRLEQES-----YGLTTAEDIRRRDGEAKESKRL 90

Query: 133 ADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRHK 192
            D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S + GSCR+ R  
Sbjct: 91  RDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRH 150

Query: 193 LLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEESE-- 252
           L   LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+D E+   
Sbjct: 151 LFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRC 210

Query: 253 RIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRS-----DRKIKVQRGFLTIYKSKDEES 312
           R+GRRDI +AWRGTVT  EW  DLK  LK +  +     D  +K + GFL +Y  KD   
Sbjct: 211 RLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSC 270

Query: 313 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD--- 372
            F+K SA EQV+ E+ RL++ +  +   E+S+T+ GHSLGGAL++L+AY+      +   
Sbjct: 271 NFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTR 330

Query: 373 ----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNS-IVNKLS 432
               I V+  ++G PRVGN+ F+E++ ++GVK LRVV   D+V K PGLF+N      L 
Sbjct: 331 KGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALM 390

Query: 433 AVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNS 492
            + G L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+  +  +F  +S
Sbjct: 391 KLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGYHGKGQRFVLSS 450

Query: 493 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR-NAEDIPSP 524
            RD ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R  A+D  +P
Sbjct: 451 GRDPALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDRIRADDQHAP 499

BLAST of Cp4.1LG20g05490 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 1.3e-99
Identity = 185/409 (45.23%), Postives = 263/409 (64.30%), Query Frame = 0

Query: 125 IADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNRH 184
           + ++WR++ G +NWEG LDP++  LRRE+++YGEFAQA YD+FDFDP S++ GSC+Y+  
Sbjct: 87  LREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPS 146

Query: 185 KLLHELGL-AQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 244
                L L    GY +TRY+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+ DEE  
Sbjct: 147 DFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEEV 206

Query: 245 ERIGRRDILVAWRGTVTPTEWYIDLKTKLKKID-RSDRKIKVQRGFLTIYKSKDEESKFN 304
            R+GRRDI++AWRGTVT  EW  DLK  L   +   D  IK++ GF  +Y  K++  KF+
Sbjct: 207 SRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFS 266

Query: 305 KTSASEQVMEELHRLIDFF-TQKGDREISLTIVGHSLGGALSLLTAYEAGA----SFPD- 364
             SA EQV+ E+ RLI+++ T++   + S+T+ GHSLG +L+L++AY+         P+ 
Sbjct: 267 SFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPEN 326

Query: 365 ---IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAV 424
              I ++V SF  PRVGNL F+E+ +E+GVK LRVV   D VP +PG+F N        V
Sbjct: 327 NYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYV 386

Query: 425 TGKLN--WLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSR----RGKF 484
             K +  W Y HVG +L +D   SP+LK   D+  +HNLE  LHLVDG+  +      +F
Sbjct: 387 EEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAEKRF 446

Query: 485 RWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGR 516
              ++RD+ALVNK  D L  E  +P  W Q  +KG+VKN  G+WV P R
Sbjct: 447 CLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDR 495

BLAST of Cp4.1LG20g05490 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 341.7 bits (875), Expect = 1.1e-93
Identity = 168/352 (47.73%), Postives = 236/352 (67.05%), Query Frame = 0

Query: 124 KIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRYNR 183
           ++ D WR + G  +W GL+DP+ P LR EL++YGE AQA YDAFDFDP S++ G+ R+ R
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 184 HKLLHELGLAQNGYKVTRYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEES- 243
            +    LG+  +GY+V RY+YA S ++ P++F  S+  +VWS+++NWMG+VAVS+DE S 
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 244 ERIGRRDILVAWRGTVTPTEWYIDLKTKLK-----KIDRSDRKIKVQRGFLTIYKSKDEE 303
            R+GRRDI +AWRGTVT  EW  DLK  LK     KI   D  +KV+ GFL +Y  KD  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTT 279

Query: 304 SKFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPD-- 363
            KF + SA EQ++ E+ RL++      D ++S+T+ GHSLGGAL++L+AY+      +  
Sbjct: 280 CKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRS 339

Query: 364 -----IHVSVMSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVN-SIVNKL 423
                I V+V+++G PRVGN+ FRE++ E+GVK +RVV   D+VPK PGLF+N S  + L
Sbjct: 340 KKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHAL 399

Query: 424 SAVTGKLNWLYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVS 462
             +   L W Y HVG +L +D   SP+LK   D+S +HNLE  LHL+DG+VS
Sbjct: 400 MKIAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3EBR67.6e-10847.47Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3... [more]
Q941F12.9e-10742.41Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4... [more]
Q9C8J61.9e-9845.23Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2... [more]
B9EYD32.8e-7039.85Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 P... [more]
O235221.8e-6935.02Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16... [more]
Match NameE-valueIdentityDescription
XP_023519232.10.0100.00phospholipase A1-Igamma1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7019492.10.099.43Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
KAG6583869.10.099.25Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022927629.10.099.25phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata][more]
XP_023001410.10.097.92phospholipase A1-Igamma1, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1EIJ30.099.25phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KL460.097.92phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5A7UFJ90.087.13Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3BBX30.087.13phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 ... [more]
A0A0A0M1110.086.73Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE... [more]
Match NameE-valueIdentityDescription
AT1G30370.14.3e-16756.73alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.25.4e-10947.47alpha/beta-Hydrolases superfamily protein [more]
AT1G06800.12.0e-10842.41alpha/beta-Hydrolases superfamily protein [more]
AT1G51440.11.3e-9945.23alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.11.1e-9347.73alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 128..522
e-value: 1.2E-125
score: 421.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 222..457
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 252..400
e-value: 1.2E-32
score: 112.7
NoneNo IPR availablePANTHERPTHR31403:SF13ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 12..527
NoneNo IPR availablePANTHERPTHR31403PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTICcoord: 12..527
NoneNo IPR availableCDDcd00519Lipase_3coord: 182..457
e-value: 1.81177E-46
score: 160.334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g05490.1Cp4.1LG20g05490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004620 phospholipase activity