Cp4.1LG20g05050 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g05050 vs. ExPASy Swiss-Prot
Match: Q39128 (Potassium channel KAT1 OS=Arabidopsis thaliana OX=3702 GN=KAT1 PE=1 SV=2)

HSP 1 Score: 753.8 bits (1945), Expect = 1.5e-216
Identity = 394/620 (63.55%), Postives = 467/620 (75.32%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPF+FAF+ YK++A+FIID+ VN FFA+DI+LTFFVAYLDS SYLLV
Sbjct: 64  MWLVLLVIYSAWICPFQFAFITYKKDAIFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLV 123

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           D PKKIA+RYLSTWF FDVCSTAP Q +S LF     E+GF++L+MLRLWRLRRVSSLFA
Sbjct: 124 DSPKKIAIRYLSTWFAFDVCSTAPFQPLSLLFNYNGSELGFRILSMLRLWRLRRVSSLFA 183

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFW RCTKLISVTLFA+HCAGCFN+LIAD+YP+P++TWIGAV PNFKE SL
Sbjct: 184 RLEKDIRFNYFWIRCTKLISVTLFAIHCAGCFNYLIADRYPNPRKTWIGAVYPNFKEASL 243

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WN Y+TA+YWSITTLTTTGYGD HAENPREMLF IF+M+FNLGLT+YLIGNMTNLVVHWT
Sbjct: 244 WNRYVTALYWSITTLTTTGYGDFHAENPREMLFDIFFMMFNLGLTAYLIGNMTNLVVHWT 303

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTR FRDSVRAA EFASRNQLP+ IQDQMLSHICLKF+TEGLKQQ+TLN+LPKAIR SI
Sbjct: 304 SRTRTFRDSVRAASEFASRNQLPHDIQDQMLSHICLKFKTEGLKQQETLNNLPKAIRSSI 363

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           A+YLF+PIV   YLF+GVS +FLFQLVSD++AEYFPPKED+ILQ+EA TDLYILVSG+VD
Sbjct: 364 ANYLFFPIVHNIYLFQGVSRNFLFQLVSDIDAEYFPPKEDIILQNEAPTDLYILVSGAVD 423

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
               +DGHDQ  G+A  G+TFGE GVL  +PQP TVRTT LSQILRI R SL+  + ++ 
Sbjct: 424 FTVYVDGHDQFQGKAVIGETFGEVGVLYYRPQPFTVRTTELSQILRISRTSLMSAMHAHA 483

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGG-----LKKLGDEDENLGSLDEHRIEF----- 480
           DDG +IMNN FMK++  + I  D     G      K +G E+      D + ++      
Sbjct: 484 DDGRVIMNNLFMKLRGQQSIAIDDSNTSGHENRDFKSMGWEEWRDSRKDGYGLDVTNPTS 543

Query: 481 DEQLEGGENDINLSHNNKLPARDGGFRSDFISSSVENLPTS---------SYSNVSRQI- 540
           D  L    +  +     K+       R+    SS E    S          Y + S QI 
Sbjct: 544 DTALMDAIHKEDTEMVKKILKEQKIERAKVERSSSETAGRSYANDSSKKDPYCSSSNQII 603

Query: 541 ------KKRVTVFIQPRDRSTVESRYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN 595
                 +KRVT+ +        ES+ GKL+LLP+SIEEL ++A EKFG    TK+ NADN
Sbjct: 604 KPCKREEKRVTIHMMS------ESKNGKLILLPSSIEELLRLASEKFGGCNFTKITNADN 663

BLAST of Cp4.1LG20g05050 vs. ExPASy Swiss-Prot
Match: Q38849 (Potassium channel KAT2 OS=Arabidopsis thaliana OX=3702 GN=KAT2 PE=1 SV=3)

HSP 1 Score: 752.7 bits (1942), Expect = 3.3e-216
Identity = 401/640 (62.66%), Postives = 472/640 (73.75%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPFEFAF+ YK++ALFIID+ VN FFA+DI+LTFFVAYLDS SYLLV
Sbjct: 64  MWLVILVIYSAWICPFEFAFITYKKDALFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLV 123

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           D PKKIA+RYLSTWF FDVCSTAP QS+S LF     E+GF++L+MLRLWRLRRVSSLFA
Sbjct: 124 DKPKKIAIRYLSTWFAFDVCSTAPFQSLSLLFKYNGSEIGFRVLSMLRLWRLRRVSSLFA 183

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCF +LIAD+Y DP +TWIGAV PNFKE S+
Sbjct: 184 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFAYLIADQYHDPTKTWIGAVYPNFKETSV 243

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           W+ Y+TA+YWSITTLTTTGYGDLHAENPREMLF +F+MLFNLG TSYLIGNMTNLVVHWT
Sbjct: 244 WSRYVTALYWSITTLTTTGYGDLHAENPREMLFFVFFMLFNLGFTSYLIGNMTNLVVHWT 303

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRD+VRAA EFASRNQLP  IQDQMLSHICLKF+TEGLKQQ+ LN LPKAIR SI
Sbjct: 304 SRTRNFRDTVRAASEFASRNQLPPNIQDQMLSHICLKFKTEGLKQQEALNGLPKAIRSSI 363

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           A+YLF+PIVQ  YLF GVS +FLFQLVSD++AEYFPP+EDVILQ+EA TDLYILVSG+VD
Sbjct: 364 ANYLFFPIVQNVYLFHGVSRNFLFQLVSDIDAEYFPPREDVILQNEAPTDLYILVSGAVD 423

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
               +   DQV G+A  GD FGE GVLC  PQP TVRTT LSQILRI + SL+  ++++ 
Sbjct: 424 FTVYVGEEDQVQGKAVVGDAFGEIGVLCYTPQPFTVRTTELSQILRISKKSLMSAMRAHV 483

Query: 421 DDGNIIMNNFFMKMKEYERIMGD-----------VWCDGGLK----------------KL 480
           +DG +IMNN FMK++  + I  D            W  GG K                +L
Sbjct: 484 EDGRVIMNNLFMKLRGQQSIAIDDPNSEPESLLKEWLVGGSKTGEGNASDQGHGHKYLQL 543

Query: 481 GD-EDENLGSL---DEHRIEFDEQLEGGENDINLSHNNKLPARD----GGFRSDFISSSV 540
            D E+ ++GS    D  R  + E     E+ I +    K P ++    G   +D  S   
Sbjct: 544 HDSENIDMGSTEWRDSRRSGYGETKRVREHTIEIEEGEK-PNKEFDGKGCSDADLTSFEF 603

Query: 541 ENLPTSSYSNVSRQIK---------KRVTVFIQPRDRSTVESRYGKLMLLPNSIEELCKV 597
            +     Y   + QIK         KRVT+ ++ RD+        KL++LP SIEEL ++
Sbjct: 604 HSQEAYPYCRSNIQIKQHEAAKPKDKRVTIHLKSRDKD-----LSKLIILPASIEELLRL 663

BLAST of Cp4.1LG20g05050 vs. ExPASy Swiss-Prot
Match: Q6K3T2 (Potassium channel KAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0245800 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 3.1e-182
Identity = 342/659 (51.90%), Postives = 450/659 (68.29%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           ++L+ LV+YSAWICPFE AFL    + L ++++ V+ FFA+DIVLTFFVAY+DS+++LLV
Sbjct: 61  LFLIVLVVYSAWICPFELAFLRDLPSKLLLVENIVDIFFAIDIVLTFFVAYVDSKTHLLV 120

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           D  K+IA+RYLSTWFIFDVCSTAP Q I  LFT++  ++ FK+LN+LRLWRL RVSSLFA
Sbjct: 121 DDRKRIAMRYLSTWFIFDVCSTAPFQPIILLFTHKGNDIAFKVLNLLRLWRLHRVSSLFA 180

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRC+KLISVTLFAVHCAGCFN++IAD+YP+P++TWIGAV   F+ +SL
Sbjct: 181 RLEKDIRFNYFWTRCSKLISVTLFAVHCAGCFNYMIADRYPNPEKTWIGAVMSTFRSESL 240

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           W  YITA+YWSITTLTTTGYGDLHAENP EMLF I YM+FNLGLT+YLIGNMTNLVVH T
Sbjct: 241 WTRYITALYWSITTLTTTGYGDLHAENPTEMLFDIVYMMFNLGLTAYLIGNMTNLVVHGT 300

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTR FRDS++AA EFA+RNQLP  I+ Q+LSH CL+F+TEGL QQ  L+ LPK IR SI
Sbjct: 301 SRTRKFRDSIQAASEFAARNQLPENIKQQVLSHFCLQFKTEGLNQQVMLDCLPKGIRSSI 360

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           A+ LF+PI+++AYLF GVS +F+ +LV +V+AEYFPPKED+ILQ+E + D+YI+VSG+V+
Sbjct: 361 AYSLFFPIIRQAYLFNGVSGNFIAELVMEVQAEYFPPKEDIILQNEGEADVYIVVSGAVN 420

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           +I+ I G++QV  +   G+ FGE G LC  PQP T RT  LSQ+LRI +  L  II+ N 
Sbjct: 421 IITTIHGNEQVYEKIAEGEMFGEVGSLCNIPQPFTCRTAELSQLLRISKTRLREIIEENR 480

Query: 421 DDGNIIMNNFFMKMK-----------------EYE--RIMGDVWCDGGLKK--------- 480
           +D NI+MNN   K+K                 +YE   +  + W    LKK         
Sbjct: 481 EDSNILMNNLVQKLKLRESLPDMNQPDRRFLSKYELFHVPREAWL---LKKSQLHYTEHT 540

Query: 481 --------------------LGDEDENLGSLDEHRIEFDEQLEGGENDINL-------SH 540
                               LG+   +  S +E+    D++    E   N         H
Sbjct: 541 SRDSSNNTPVFGGDRYSRQLLGEATRSSASENENSSMTDKEENHDEVHTNCEIKKRTEEH 600

Query: 541 NNKLPARDGG--FRSDFISSSVE-NLPTSSYSNVSRQI---------KKRVTVFIQPRDR 593
             ++ + D    +    ++++V+   P  +  N++R+I          KRVT+     + 
Sbjct: 601 CIQINSEDSSSTYSQRTMNATVQTGSPHKTEENITRRIPDEYYIKEANKRVTIHKYRHNS 660

BLAST of Cp4.1LG20g05050 vs. ExPASy Swiss-Prot
Match: Q5JM04 (Potassium channel KAT3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0756700 PE=2 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 2.0e-173
Identity = 296/428 (69.16%), Postives = 358/428 (83.64%), Query Frame = 0

Query: 2   WLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLVD 61
           +L+ LV+YSAWICP EFAFL Y  +A F++D  VN FFAVDI+LTFFV ++D +SYLLV+
Sbjct: 69  FLIILVVYSAWICPLEFAFLRYLPSAPFVVDDVVNGFFAVDIMLTFFVPFVDKKSYLLVN 128

Query: 62  HPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFAR 121
            PKKIAVRYLS+WF+FDVCST P  SIS LF     ++GFK LN+LRLWRLRRVSS+FAR
Sbjct: 129 DPKKIAVRYLSSWFVFDVCSTVPFHSISLLFNEHGHDLGFKFLNVLRLWRLRRVSSMFAR 188

Query: 122 LEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSLW 181
           LEKDIRFNY   RCTKLISVTLFA+HCAGC N+LIAD+YPDP+RTWIGAV PNF+ED LW
Sbjct: 189 LEKDIRFNYAVIRCTKLISVTLFAIHCAGCINYLIADRYPDPRRTWIGAVMPNFREDGLW 248

Query: 182 NLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWTS 241
             Y+TA+YWSITTLTTTGYGDLHAEN REMLF I YMLFNL LT+YLIGNMTNLVVH TS
Sbjct: 249 IRYVTAMYWSITTLTTTGYGDLHAENAREMLFGICYMLFNLWLTAYLIGNMTNLVVHSTS 308

Query: 242 RTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSIA 301
           RTR+FRD V+AA EFA+RNQLP  I++QML+HICL+++T+GLKQQ+TL+ LPKA+R SI+
Sbjct: 309 RTRDFRDVVQAASEFAARNQLPQQIEEQMLNHICLRYKTDGLKQQETLDVLPKAMRSSIS 368

Query: 302 HYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVDL 361
           HYLF+ +VQ AYLF+GVS  F+ QLV++++AEYF PKED+ILQ+++ +DLY+LVSG+VD+
Sbjct: 369 HYLFFRVVQGAYLFKGVSSRFIQQLVTEMQAEYFAPKEDIILQNDSPSDLYLLVSGAVDI 428

Query: 362 ISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNTD 421
           +  +DG +QV  RA  G+  GE GVLC KPQ  T RTT LSQILRI R  LL IIQ N +
Sbjct: 429 LVFLDGTEQVYRRAAEGELLGEIGVLCNKPQSFTFRTTKLSQILRISRTKLLGIIQENRE 488

Query: 422 DGNIIMNN 430
           DG+II +N
Sbjct: 489 DGDIIRSN 496

BLAST of Cp4.1LG20g05050 vs. ExPASy Swiss-Prot
Match: Q5QNI1 (Potassium channel KAT2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0210700 PE=2 SV=2)

HSP 1 Score: 600.5 bits (1547), Expect = 2.1e-170
Identity = 308/591 (52.12%), Postives = 414/591 (70.05%), Query Frame = 0

Query: 2   WLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLVD 61
           +L+ LV+YSAWICPFE A+L      + ++D+ +++FFA+DI+LTFF+AYLD +SYLLVD
Sbjct: 44  FLLVLVVYSAWICPFELAYLRNLSWKVSLVDNIIDSFFAIDIILTFFLAYLDQKSYLLVD 103

Query: 62  HPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFAR 121
            PK+I  RY S+WF+FDVCST P Q +  +F      + ++LL+MLRLWRLRR+S LFAR
Sbjct: 104 DPKRIVARYFSSWFLFDVCSTIPYQLLGQIFKKHENGLAYRLLSMLRLWRLRRLSELFAR 163

Query: 122 LEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSLW 181
           LEKDIR NY+W RCTKLISVTLFAVHC+GCFN+LIAD+YP+P RTWIGA  PN++  +LW
Sbjct: 164 LEKDIRLNYYWIRCTKLISVTLFAVHCSGCFNYLIADRYPNPARTWIGAAIPNYRSQNLW 223

Query: 182 NLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWTS 241
             Y+TAIYWSITTLTTTGYGDLHAEN REMLF+I YMLFNLGLT+YLIGNMTNLVV  + 
Sbjct: 224 VRYVTAIYWSITTLTTTGYGDLHAENQREMLFSICYMLFNLGLTAYLIGNMTNLVVQGSC 283

Query: 242 RTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSIA 301
           RTRNFRD++ AA +FA+RNQLP  I+D+MLSHICL+++TEGLKQ++TL+ LPK IR SIA
Sbjct: 284 RTRNFRDTIHAASQFAARNQLPGHIKDEMLSHICLRYKTEGLKQKETLDSLPKGIRSSIA 343

Query: 302 HYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVDL 361
             LF P+++K YLF GVS   + QLV+++EAEY+PP+E VILQ+EA  D+YILVSG+V+ 
Sbjct: 344 CNLFLPVIEKVYLFHGVSFTCMIQLVTEMEAEYYPPREVVILQNEAPRDVYILVSGAVEE 403

Query: 362 ISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNTD 421
             +IDG ++V      G+ FGE GV+C  PQP    T  +SQ+LR+  A L  II+ N+D
Sbjct: 404 RVEIDGTEKVQEVLCNGEIFGEIGVICSIPQPCAFHTIKVSQLLRLNTAVLKNIIKENSD 463

Query: 422 DGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGENDI 481
           D  +I+NN   KM +  R   +V            +++L  + +H  E++      ++  
Sbjct: 464 DRRVILNNLSQKMNQDHRFSTEVM-----------EKSLQMMHQHFGEYNRCSALNQD-- 523

Query: 482 NLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRYG 541
             +  N+L A +G       S ++E               KRVT+ +  +     E+   
Sbjct: 524 --NEKNELKANNGH------SMALE--------------WKRVTIHMYSQRNKRPEAPLA 583

Query: 542 KLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLL 593
           K++ LP S+++L  +A +KF     TK++N + +EIDDI VIRDGDHLF +
Sbjct: 584 KVINLPGSLDKLFAIACQKFNNYRLTKLVNPEFAEIDDITVIRDGDHLFFM 599

BLAST of Cp4.1LG20g05050 vs. NCBI nr
Match: XP_023519285.1 (potassium channel KAT1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1212 bits (3135), Expect = 0.0
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 480
           DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND
Sbjct: 485 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 544

Query: 481 INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 540
           INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY
Sbjct: 545 INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 604

Query: 541 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC 600
           GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC
Sbjct: 605 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC 664

Query: 601 GLK 603
           GLK
Sbjct: 665 GLK 667

BLAST of Cp4.1LG20g05050 vs. NCBI nr
Match: XP_022927077.1 (potassium channel KAT1-like [Cucurbita moschata])

HSP 1 Score: 1194 bits (3090), Expect = 0.0
Identity = 595/603 (98.67%), Postives = 599/603 (99.34%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFN+LIADKYPDPKRTWIGAV+PNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNYLIADKYPDPKRTWIGAVNPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAA EFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAATEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 480
           DDGNIIMNNFFMKMKEYE IMG+VWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND
Sbjct: 485 DDGNIIMNNFFMKMKEYEGIMGNVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 544

Query: 481 INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 540
           INLSHNNKL ARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY
Sbjct: 545 INLSHNNKLLARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 604

Query: 541 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC 600
           GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN+EIDDINVIRDGDHLFLLYNNDIEKC
Sbjct: 605 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNAEIDDINVIRDGDHLFLLYNNDIEKC 664

Query: 601 GLK 603
           GLK
Sbjct: 665 GLK 667

BLAST of Cp4.1LG20g05050 vs. NCBI nr
Match: KAG7019531.1 (Potassium channel KAT1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1190 bits (3078), Expect = 0.0
Identity = 593/603 (98.34%), Postives = 596/603 (98.84%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFN+LIADKYPDPKRTWIGAV+PNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNYLIADKYPDPKRTWIGAVNPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAA EFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAATEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 480
           DDGNIIMNNFFMKMKEYE IMGDVWCDGGLKKLGDEDENLG  DEHRIEFDEQLEGGEND
Sbjct: 485 DDGNIIMNNFFMKMKEYEGIMGDVWCDGGLKKLGDEDENLGCSDEHRIEFDEQLEGGEND 544

Query: 481 INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 540
           INLSHNNKL ARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRS VESRY
Sbjct: 545 INLSHNNKLLARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSKVESRY 604

Query: 541 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC 600
           GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN+EIDDINVIRDGDHLFLLYNNDIEKC
Sbjct: 605 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNAEIDDINVIRDGDHLFLLYNNDIEKC 664

Query: 601 GLK 603
           GLK
Sbjct: 665 GLK 667

BLAST of Cp4.1LG20g05050 vs. NCBI nr
Match: KAG6583917.1 (Potassium channel KAT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1186 bits (3067), Expect = 0.0
Identity = 591/603 (98.01%), Postives = 595/603 (98.67%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRC KLISVTLFAVHCAGCFN+LIADKYPDPKRTWIGAV+PNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCIKLISVTLFAVHCAGCFNYLIADKYPDPKRTWIGAVNPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAA EFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAATEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 480
           DDGNIIMNNFFMKMKEYE IMGDVWCDGGLKKLGDEDENLG LDEHRIEFDEQLEGGEND
Sbjct: 485 DDGNIIMNNFFMKMKEYEGIMGDVWCDGGLKKLGDEDENLGCLDEHRIEFDEQLEGGEND 544

Query: 481 INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 540
           INLSHNNKL ARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRS VESRY
Sbjct: 545 INLSHNNKLLARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSKVESRY 604

Query: 541 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC 600
           GKLMLLPNSIEELCKVAGEKFGR MPTKVMNADN+EIDDINVIRDGDHLFLLYNNDI+KC
Sbjct: 605 GKLMLLPNSIEELCKVAGEKFGRGMPTKVMNADNAEIDDINVIRDGDHLFLLYNNDIKKC 664

Query: 601 GLK 603
           GLK
Sbjct: 665 GLK 667

BLAST of Cp4.1LG20g05050 vs. NCBI nr
Match: XP_023001404.1 (potassium channel KAT3-like [Cucurbita maxima])

HSP 1 Score: 1171 bits (3029), Expect = 0.0
Identity = 585/607 (96.38%), Postives = 594/607 (97.86%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQN+LFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNSLFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAV+PNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVNPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAA EFASRNQLPNPIQ+QMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAATEFASRNQLPNPIQNQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQL SDVEAEYFPPKEDVILQ+EAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLASDVEAEYFPPKEDVILQNEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGE GVLCQKPQP TVRTTNLSQILRIKRASLLYIIQSN 
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGELGVLCQKPQPFTVRTTNLSQILRIKRASLLYIIQSNA 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGD----EDENLGSLDEHRIEFDEQLEG 480
           +DGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGD    EDENLGSLDEHRIEFDEQLEG
Sbjct: 485 EDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDQCEDEDENLGSLDEHRIEFDEQLEG 544

Query: 481 GENDINLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTV 540
           GENDINLSHNNKL AR+GG RSDFISSS+ENLPTSSYSNVSRQIKKRVTVFIQPRDRS V
Sbjct: 545 GENDINLSHNNKLLARNGGGRSDFISSSLENLPTSSYSNVSRQIKKRVTVFIQPRDRSKV 604

Query: 541 ESRYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNND 600
           ES+YGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN+EIDDINVIRDGDHLFLLYNND
Sbjct: 605 ESQYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNAEIDDINVIRDGDHLFLLYNND 664

Query: 601 IEKCGLK 603
           IEKCGLK
Sbjct: 665 IEKCGLK 671

BLAST of Cp4.1LG20g05050 vs. ExPASy TrEMBL
Match: A0A6J1EG46 (potassium channel KAT1-like OS=Cucurbita moschata OX=3662 GN=LOC111434011 PE=3 SV=1)

HSP 1 Score: 1194 bits (3090), Expect = 0.0
Identity = 595/603 (98.67%), Postives = 599/603 (99.34%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFN+LIADKYPDPKRTWIGAV+PNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNYLIADKYPDPKRTWIGAVNPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAA EFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAATEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 480
           DDGNIIMNNFFMKMKEYE IMG+VWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND
Sbjct: 485 DDGNIIMNNFFMKMKEYEGIMGNVWCDGGLKKLGDEDENLGSLDEHRIEFDEQLEGGEND 544

Query: 481 INLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 540
           INLSHNNKL ARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY
Sbjct: 545 INLSHNNKLLARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTVESRY 604

Query: 541 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNNDIEKC 600
           GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN+EIDDINVIRDGDHLFLLYNNDIEKC
Sbjct: 605 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNAEIDDINVIRDGDHLFLLYNNDIEKC 664

Query: 601 GLK 603
           GLK
Sbjct: 665 GLK 667

BLAST of Cp4.1LG20g05050 vs. ExPASy TrEMBL
Match: A0A6J1KMM4 (potassium channel KAT3-like OS=Cucurbita maxima OX=3661 GN=LOC111495547 PE=3 SV=1)

HSP 1 Score: 1171 bits (3029), Expect = 0.0
Identity = 585/607 (96.38%), Postives = 594/607 (97.86%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLVALVIYSAWICPFEFAFLPYKQN+LFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV
Sbjct: 65  MWLVALVIYSAWICPFEFAFLPYKQNSLFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 124

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 125 DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 184

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAV+PNFKEDSL
Sbjct: 185 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVNPNFKEDSL 244

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYITAIYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLGLTSYLIGNMTNLVVHWT
Sbjct: 245 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGLTSYLIGNMTNLVVHWT 304

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSVRAA EFASRNQLPNPIQ+QMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI
Sbjct: 305 SRTRNFRDSVRAATEFASRNQLPNPIQNQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 364

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQL SDVEAEYFPPKEDVILQ+EAQTDLYILVSGSVD
Sbjct: 365 AHYLFYPIVQKAYLFEGVSHDFLFQLASDVEAEYFPPKEDVILQNEAQTDLYILVSGSVD 424

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
           LISKIDGHDQVIGRATGGDTFGE GVLCQKPQP TVRTTNLSQILRIKRASLLYIIQSN 
Sbjct: 425 LISKIDGHDQVIGRATGGDTFGELGVLCQKPQPFTVRTTNLSQILRIKRASLLYIIQSNA 484

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGD----EDENLGSLDEHRIEFDEQLEG 480
           +DGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGD    EDENLGSLDEHRIEFDEQLEG
Sbjct: 485 EDGNIIMNNFFMKMKEYERIMGDVWCDGGLKKLGDQCEDEDENLGSLDEHRIEFDEQLEG 544

Query: 481 GENDINLSHNNKLPARDGGFRSDFISSSVENLPTSSYSNVSRQIKKRVTVFIQPRDRSTV 540
           GENDINLSHNNKL AR+GG RSDFISSS+ENLPTSSYSNVSRQIKKRVTVFIQPRDRS V
Sbjct: 545 GENDINLSHNNKLLARNGGGRSDFISSSLENLPTSSYSNVSRQIKKRVTVFIQPRDRSKV 604

Query: 541 ESRYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDINVIRDGDHLFLLYNND 600
           ES+YGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN+EIDDINVIRDGDHLFLLYNND
Sbjct: 605 ESQYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNAEIDDINVIRDGDHLFLLYNND 664

Query: 601 IEKCGLK 603
           IEKCGLK
Sbjct: 665 IEKCGLK 671

BLAST of Cp4.1LG20g05050 vs. ExPASy TrEMBL
Match: Q0R4Q4 (Inward rectifying potassium channel OS=Cucumis melo OX=3656 GN=mirk PE=2 SV=2)

HSP 1 Score: 1010 bits (2612), Expect = 0.0
Identity = 521/633 (82.31%), Postives = 568/633 (89.73%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFA+DI+LTFFVAYLDSQSYLLV
Sbjct: 70  MWLVVLVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAIDIILTFFVAYLDSQSYLLV 129

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIA+RYLSTWFIFDVCSTAPLQSISFLFTNQ+GEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 130 DHPKKIALRYLSTWFIFDVCSTAPLQSISFLFTNQTGEVGFKLLNMLRLWRLRRVSSLFA 189

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFN++IADKYPD K+TWIGAV+PNFKE+SL
Sbjct: 190 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNYVIADKYPDSKQTWIGAVNPNFKEESL 249

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYIT+IYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLG TSYLIGNMTNLVVHWT
Sbjct: 250 WNLYITSIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGFTSYLIGNMTNLVVHWT 309

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSV+AA EFA RNQLP  IQDQMLSHICLKFRTEGLKQQDTLN+LPKAIR SI
Sbjct: 310 SRTRNFRDSVQAATEFALRNQLPERIQDQMLSHICLKFRTEGLKQQDTLNELPKAIRASI 369

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKED+ILQ+EAQTDLYILVSGSVD
Sbjct: 370 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDIILQNEAQTDLYILVSGSVD 429

Query: 361 LI-SKIDGHD---QVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYII 420
           LI S +DGHD   QVIGR T GD FGE+GVLCQKPQP T+RTT LSQILR+KR SLLYII
Sbjct: 430 LILSHVDGHDDHDQVIGRGTSGDMFGEYGVLCQKPQPFTIRTTKLSQILRLKRTSLLYII 489

Query: 421 QSNTDDGNIIMNNFFMKMKEYERIMGDVWCDGG-LKKL--------GDEDENLG----SL 480
           QSNT+DGNIIMNNFFMKMKEYER++G++WCD G LKKL         DE+ N+G    +L
Sbjct: 490 QSNTEDGNIIMNNFFMKMKEYERMLGNIWCDNGELKKLENRYSKEDDDENYNMGINDNNL 549

Query: 481 DEHRIEFD--EQLEGGE-NDINLSHNNKL-----PARDGGFRSDFISSSVENLPT---SS 540
           DEHRIEFD  E++EGGE N I  SHNNKL        +GG  SD ISSS+ENLPT   S+
Sbjct: 550 DEHRIEFDDHEEVEGGEKNIIKFSHNNKLLQGRNSNNNGGQTSDIISSSLENLPTISSSN 609

Query: 541 YSNVSRQIKKRVTVFIQPRDRST-VESRYGKLMLLPNSIEELCKVAGEKFGRE-MPTKVM 600
           Y N S+QIKKRVTV++QP+DRS  V+S+ GKL+LLP++IEELCK+AGEKFG + MPTKVM
Sbjct: 610 YLNSSKQIKKRVTVYMQPQDRSCKVKSQLGKLILLPDTIEELCKIAGEKFGGQIMPTKVM 669

Query: 601 NADNSEIDDINVIRDGDHLFLLYNNDIEKCGLK 603
           +ADN+EIDDINVIRDGDHLFLLYN+ I+ CGL+
Sbjct: 670 SADNAEIDDINVIRDGDHLFLLYNH-IQNCGLQ 701

BLAST of Cp4.1LG20g05050 vs. ExPASy TrEMBL
Match: A0A7R6LUC6 (Low affinity inward rectifying potassium channel KAT2 OS=Citrullus lanatus OX=3654 GN=KAT2 PE=2 SV=1)

HSP 1 Score: 1010 bits (2612), Expect = 0.0
Identity = 519/622 (83.44%), Postives = 564/622 (90.68%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFA+DI+LTFFVAYLDSQSYLL+
Sbjct: 71  MWLVVLVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAIDIILTFFVAYLDSQSYLLI 130

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIA+RYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 131 DHPKKIALRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 190

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFN++IADKYPD K+TWIGAV+PNFKEDS+
Sbjct: 191 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNYVIADKYPDSKQTWIGAVNPNFKEDSV 250

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYIT++YWSITTLTTTGYGDLHAENPREMLF IFYMLFNLG TSYLIGNMTNLVVHWT
Sbjct: 251 WNLYITSLYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGFTSYLIGNMTNLVVHWT 310

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSV+AA EFA RNQLP  IQDQMLSHICLKFRTEGLKQQDTLNDLPKAIR SI
Sbjct: 311 SRTRNFRDSVQAATEFALRNQLPERIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRASI 370

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPI+QKAYLFEGVSHDFLFQLVSDVEAEYFPPKED+ILQ+EAQTDLYILVSGSVD
Sbjct: 371 AHYLFYPILQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDIILQNEAQTDLYILVSGSVD 430

Query: 361 LI--SKIDGHD-QVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQ 420
           LI  +  DG D QVIG AT G+ FGEFGVLCQKPQP TVRTT LSQILR+KR SLLYI+Q
Sbjct: 431 LILSNNEDGQDEQVIGGATAGEMFGEFGVLCQKPQPFTVRTTKLSQILRLKRTSLLYILQ 490

Query: 421 SNTDDGNIIMNNFFMKMKEYERIMGDVWCDGG--LKKLG----DEDEN-LGS----LDEH 480
           SNT+DGNIIMNNFFMKMKEY RIMG++WCD G  LKK+     ++DEN +GS    LDEH
Sbjct: 491 SNTEDGNIIMNNFFMKMKEYGRIMGNIWCDNGELLKKVENYSKEDDENYVGSNNNNLDEH 550

Query: 481 RIEFDEQLEGGENDI-NLSHNNKLPARDGGFRSDFISSSVENLPT--SSY-SNVSRQIKK 540
           RIEFDE++EGGE +I   SHNNKL  R+ G +      S+EN PT  S+Y +NVSRQIKK
Sbjct: 551 RIEFDEEVEGGEKEIIKFSHNNKLQGRNNGGQP-----SLENFPTVSSNYLNNVSRQIKK 610

Query: 541 RVTVFIQ-PRDRSTVESRYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADNSEIDDIN 600
           RVTV++Q P+DRS V+S+YGKLMLLPNSIEELCK+AGEKFG +MPTKVM+ADN+EIDDIN
Sbjct: 611 RVTVYMQHPQDRSKVKSQYGKLMLLPNSIEELCKIAGEKFGGQMPTKVMSADNAEIDDIN 670

Query: 601 VIRDGDHLFLLYNNDIEKCGLK 603
           VIRDGDHLFLLY+ DIE CGL+
Sbjct: 671 VIRDGDHLFLLYS-DIENCGLQ 686

BLAST of Cp4.1LG20g05050 vs. ExPASy TrEMBL
Match: A0A7R6LUC7 (Low affnity inward rectifying potassium channel KAT2 OS=Cucumis sativus OX=3659 GN=KAT2 PE=2 SV=1)

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 506/632 (80.06%), Postives = 555/632 (87.82%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPFEFAFLPYKQNALF+ID FVNAFFA+DI+LTFFVAYLDSQSYLLV
Sbjct: 69  MWLVVLVIYSAWICPFEFAFLPYKQNALFLIDQFVNAFFAIDIILTFFVAYLDSQSYLLV 128

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           DHPKKIA+RYLSTWFIFDVCSTAPLQSISFLFTN++GEVGFKLLNMLRLWRLRRVSSLFA
Sbjct: 129 DHPKKIALRYLSTWFIFDVCSTAPLQSISFLFTNETGEVGFKLLNMLRLWRLRRVSSLFA 188

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFN++IADKYPD K+TWIGAV+PNFKE+SL
Sbjct: 189 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNYVIADKYPDSKQTWIGAVNPNFKEESL 248

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WNLYIT+IYWSITTLTTTGYGDLHAENPREMLF IFYMLFNLG TSYLIGNMTNLVVHWT
Sbjct: 249 WNLYITSIYWSITTLTTTGYGDLHAENPREMLFDIFYMLFNLGFTSYLIGNMTNLVVHWT 308

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRDSV AA EFA RNQLP  IQDQMLSHICLKFRTEGLKQQD LN+LPKAIR SI
Sbjct: 309 SRTRNFRDSVEAATEFALRNQLPERIQDQMLSHICLKFRTEGLKQQDPLNELPKAIRASI 368

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKED+ILQ+EAQTDLYILVSGSVD
Sbjct: 369 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDIILQNEAQTDLYILVSGSVD 428

Query: 361 LI-SKIDGHD--QVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQ 420
           LI S +DGHD  QVIGRAT GD FGEFGVLCQKPQP T++TT LSQILR+KR SLLYI Q
Sbjct: 429 LILSHVDGHDHDQVIGRATSGDMFGEFGVLCQKPQPFTIKTTKLSQILRLKRTSLLYIFQ 488

Query: 421 SNTDDGNIIMNNFFMKMKEYERIMGDVWCD-GGLKKLG-------DEDENLG------SL 480
           SNT+DGNIIMNNFFMKMKEYERI+G++WCD GGLK+L        D+DEN+        L
Sbjct: 489 SNTEDGNIIMNNFFMKMKEYERILGNIWCDNGGLKELDQNHYSKEDDDENINMGIDDNEL 548

Query: 481 DEHRIEFDEQLEGGEND--INLSHNNKLPA------RDGGFRSDFISSSVENLP---TSS 540
           DEHRIEF+E+  GG+    I  SHNN+L        ++GG  SD ++SS ENL    +  
Sbjct: 549 DEHRIEFEEEEVGGDEKEIIKYSHNNRLLEGRNNNNKEGGKTSDLMNSSSENLGRMRSCK 608

Query: 541 YSNVSRQIKKRVTVFIQPRDRSTVESRYGKLMLLPNSIEELCKVAGEKFGRE-MPTKVMN 600
             NVS+QIKKRVTV++QP DRS V+S  GKL+LLP+SI++LCK+AGEKFG E M TKVM+
Sbjct: 609 NLNVSKQIKKRVTVYVQPEDRSKVKSEIGKLILLPDSIQQLCKIAGEKFGGEIMATKVMS 668

Query: 601 ADNSEIDDINVIRDGDHLFLLYNNDIEKCGLK 603
           ADN+EIDDINVIRDGD LFLLYN+ I+ CGL+
Sbjct: 669 ADNAEIDDINVIRDGDRLFLLYNH-IQNCGLQ 699

BLAST of Cp4.1LG20g05050 vs. TAIR 10
Match: AT5G46240.1 (potassium channel in Arabidopsis thaliana 1 )

HSP 1 Score: 753.8 bits (1945), Expect = 1.0e-217
Identity = 394/620 (63.55%), Postives = 467/620 (75.32%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPF+FAF+ YK++A+FIID+ VN FFA+DI+LTFFVAYLDS SYLLV
Sbjct: 64  MWLVLLVIYSAWICPFQFAFITYKKDAIFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLV 123

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           D PKKIA+RYLSTWF FDVCSTAP Q +S LF     E+GF++L+MLRLWRLRRVSSLFA
Sbjct: 124 DSPKKIAIRYLSTWFAFDVCSTAPFQPLSLLFNYNGSELGFRILSMLRLWRLRRVSSLFA 183

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFW RCTKLISVTLFA+HCAGCFN+LIAD+YP+P++TWIGAV PNFKE SL
Sbjct: 184 RLEKDIRFNYFWIRCTKLISVTLFAIHCAGCFNYLIADRYPNPRKTWIGAVYPNFKEASL 243

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           WN Y+TA+YWSITTLTTTGYGD HAENPREMLF IF+M+FNLGLT+YLIGNMTNLVVHWT
Sbjct: 244 WNRYVTALYWSITTLTTTGYGDFHAENPREMLFDIFFMMFNLGLTAYLIGNMTNLVVHWT 303

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTR FRDSVRAA EFASRNQLP+ IQDQMLSHICLKF+TEGLKQQ+TLN+LPKAIR SI
Sbjct: 304 SRTRTFRDSVRAASEFASRNQLPHDIQDQMLSHICLKFKTEGLKQQETLNNLPKAIRSSI 363

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           A+YLF+PIV   YLF+GVS +FLFQLVSD++AEYFPPKED+ILQ+EA TDLYILVSG+VD
Sbjct: 364 ANYLFFPIVHNIYLFQGVSRNFLFQLVSDIDAEYFPPKEDIILQNEAPTDLYILVSGAVD 423

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
               +DGHDQ  G+A  G+TFGE GVL  +PQP TVRTT LSQILRI R SL+  + ++ 
Sbjct: 424 FTVYVDGHDQFQGKAVIGETFGEVGVLYYRPQPFTVRTTELSQILRISRTSLMSAMHAHA 483

Query: 421 DDGNIIMNNFFMKMKEYERIMGDVWCDGG-----LKKLGDEDENLGSLDEHRIEF----- 480
           DDG +IMNN FMK++  + I  D     G      K +G E+      D + ++      
Sbjct: 484 DDGRVIMNNLFMKLRGQQSIAIDDSNTSGHENRDFKSMGWEEWRDSRKDGYGLDVTNPTS 543

Query: 481 DEQLEGGENDINLSHNNKLPARDGGFRSDFISSSVENLPTS---------SYSNVSRQI- 540
           D  L    +  +     K+       R+    SS E    S          Y + S QI 
Sbjct: 544 DTALMDAIHKEDTEMVKKILKEQKIERAKVERSSSETAGRSYANDSSKKDPYCSSSNQII 603

Query: 541 ------KKRVTVFIQPRDRSTVESRYGKLMLLPNSIEELCKVAGEKFGREMPTKVMNADN 595
                 +KRVT+ +        ES+ GKL+LLP+SIEEL ++A EKFG    TK+ NADN
Sbjct: 604 KPCKREEKRVTIHMMS------ESKNGKLILLPSSIEELLRLASEKFGGCNFTKITNADN 663

BLAST of Cp4.1LG20g05050 vs. TAIR 10
Match: AT4G18290.1 (potassium channel in Arabidopsis thaliana 2 )

HSP 1 Score: 752.7 bits (1942), Expect = 2.3e-217
Identity = 401/640 (62.66%), Postives = 472/640 (73.75%), Query Frame = 0

Query: 1   MWLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLV 60
           MWLV LVIYSAWICPFEFAF+ YK++ALFIID+ VN FFA+DI+LTFFVAYLDS SYLLV
Sbjct: 64  MWLVILVIYSAWICPFEFAFITYKKDALFIIDNIVNGFFAIDIILTFFVAYLDSHSYLLV 123

Query: 61  DHPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFA 120
           D PKKIA+RYLSTWF FDVCSTAP QS+S LF     E+GF++L+MLRLWRLRRVSSLFA
Sbjct: 124 DKPKKIAIRYLSTWFAFDVCSTAPFQSLSLLFKYNGSEIGFRVLSMLRLWRLRRVSSLFA 183

Query: 121 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSL 180
           RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCF +LIAD+Y DP +TWIGAV PNFKE S+
Sbjct: 184 RLEKDIRFNYFWTRCTKLISVTLFAVHCAGCFAYLIADQYHDPTKTWIGAVYPNFKETSV 243

Query: 181 WNLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWT 240
           W+ Y+TA+YWSITTLTTTGYGDLHAENPREMLF +F+MLFNLG TSYLIGNMTNLVVHWT
Sbjct: 244 WSRYVTALYWSITTLTTTGYGDLHAENPREMLFFVFFMLFNLGFTSYLIGNMTNLVVHWT 303

Query: 241 SRTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRTEGLKQQDTLNDLPKAIRVSI 300
           SRTRNFRD+VRAA EFASRNQLP  IQDQMLSHICLKF+TEGLKQQ+ LN LPKAIR SI
Sbjct: 304 SRTRNFRDTVRAASEFASRNQLPPNIQDQMLSHICLKFKTEGLKQQEALNGLPKAIRSSI 363

Query: 301 AHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSVD 360
           A+YLF+PIVQ  YLF GVS +FLFQLVSD++AEYFPP+EDVILQ+EA TDLYILVSG+VD
Sbjct: 364 ANYLFFPIVQNVYLFHGVSRNFLFQLVSDIDAEYFPPREDVILQNEAPTDLYILVSGAVD 423

Query: 361 LISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSNT 420
               +   DQV G+A  GD FGE GVLC  PQP TVRTT LSQILRI + SL+  ++++ 
Sbjct: 424 FTVYVGEEDQVQGKAVVGDAFGEIGVLCYTPQPFTVRTTELSQILRISKKSLMSAMRAHV 483

Query: 421 DDGNIIMNNFFMKMKEYERIMGD-----------VWCDGGLK----------------KL 480
           +DG +IMNN FMK++  + I  D            W  GG K                +L
Sbjct: 484 EDGRVIMNNLFMKLRGQQSIAIDDPNSEPESLLKEWLVGGSKTGEGNASDQGHGHKYLQL 543

Query: 481 GD-EDENLGSL---DEHRIEFDEQLEGGENDINLSHNNKLPARD----GGFRSDFISSSV 540
            D E+ ++GS    D  R  + E     E+ I +    K P ++    G   +D  S   
Sbjct: 544 HDSENIDMGSTEWRDSRRSGYGETKRVREHTIEIEEGEK-PNKEFDGKGCSDADLTSFEF 603

Query: 541 ENLPTSSYSNVSRQIK---------KRVTVFIQPRDRSTVESRYGKLMLLPNSIEELCKV 597
            +     Y   + QIK         KRVT+ ++ RD+        KL++LP SIEEL ++
Sbjct: 604 HSQEAYPYCRSNIQIKQHEAAKPKDKRVTIHLKSRDKD-----LSKLIILPASIEELLRL 663

BLAST of Cp4.1LG20g05050 vs. TAIR 10
Match: AT2G26650.1 (K+ transporter 1 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-152
Identity = 266/437 (60.87%), Postives = 332/437 (75.97%), Query Frame = 0

Query: 2   WLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLVD 61
           +LV LV+Y+AW+ PFEF FL   +  L I D+ VNAFFA+DI++TFFV YLD  +YL+VD
Sbjct: 63  FLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLIVD 122

Query: 62  HPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFAR 121
             K+IA +YL +WF+ D+ ST P ++ +   ++QS    + L NMLRLWRLRRV +LFAR
Sbjct: 123 DRKQIAFKYLRSWFLLDLVSTIPSEA-AMRISSQS----YGLFNMLRLWRLRRVGALFAR 182

Query: 122 LEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSLW 181
           LEKD  FNYFW RC KL+ VTLFAVHCA CF +LIA +  +P +TWIGA   NF E+SLW
Sbjct: 183 LEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLW 242

Query: 182 NLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWTS 241
             Y+T++YWSITTLTT GYGDLH  N +EM+F IFYMLFNLGLT+YLIGNMTNLVVH TS
Sbjct: 243 MRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTS 302

Query: 242 RTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRT--EGLKQQDTLNDLPKAIRVS 301
           RTRNFRD+++AA  FA RN LP  +QDQML+H+CLK+RT  EGL+QQ+TL+ LPKAIR S
Sbjct: 303 RTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSS 362

Query: 302 IAHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSV 361
           I+H+LFY ++ K YLF GVS+D LFQLVS+++AEYFPPKEDVILQ+EA TD YILV+G+ 
Sbjct: 363 ISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTA 422

Query: 362 DLISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSN 421
           DL+    G + ++     GD  GE GVLC +PQ  TVRT  L Q+LR+ R + L IIQ+N
Sbjct: 423 DLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQAN 482

Query: 422 TDDGNIIMNNFFMKMKE 437
             DG IIMNN    +KE
Sbjct: 483 VGDGTIIMNNLLQHLKE 494

HSP 2 Score: 59.3 bits (142), Expect = 1.2e-08
Identity = 31/50 (62.00%), Postives = 39/50 (78.00%), Query Frame = 0

Query: 541 GKLMLLPNSIEELCKVAGEKFGREMPTKVMNAD-NSEIDDINVIRDGDHL 590
           GKL+LLP S +EL ++   KFG  + TKVMN D N+EIDD++VIRDGDHL
Sbjct: 803 GKLVLLPGSFKELLELGSNKFG-IVATKVMNKDNNAEIDDVDVIRDGDHL 851

BLAST of Cp4.1LG20g05050 vs. TAIR 10
Match: AT2G25600.1 (Shaker pollen inward K+ channel )

HSP 1 Score: 521.9 bits (1343), Expect = 6.7e-148
Identity = 256/439 (58.31%), Postives = 329/439 (74.94%), Query Frame = 0

Query: 2   WLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLVD 61
           +LV LV+Y+AW  PFEF FL   +  L I+D+ VN FFAVDIVLTFFVA+LD  +YLLVD
Sbjct: 86  FLVFLVLYTAWASPFEFGFLQKPRPPLSILDNIVNGFFAVDIVLTFFVAFLDKVTYLLVD 145

Query: 62  HPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFAR 121
            PK+IA RY STW IFDV ST P +    L        G+ + +MLRLWRLRRVS+ FAR
Sbjct: 146 DPKRIAWRYASTWLIFDVVSTFPYEIFGSLL--HESIQGYGIFSMLRLWRLRRVSNCFAR 205

Query: 122 LEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSLW 181
           LEKD +++YFW RC+KL+ VTLF +HC  CF + IA  YPDP +T++   D N+KE  + 
Sbjct: 206 LEKDRKYSYFWVRCSKLLLVTLFVIHCGACFLYSIAAHYPDPSKTFMALTDENWKESPIA 265

Query: 182 NLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWTS 241
             Y TA+YWSITT +TTGYGD+H  N REM F +FYM+FNLGL++Y+IGNMTNLVVH T 
Sbjct: 266 VRYNTAMYWSITTFSTTGYGDIHGVNSREMTFILFYMVFNLGLSAYIIGNMTNLVVHVTG 325

Query: 242 RTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRT--EGLKQQDTLNDLPKAIRVS 301
           RTR FRD+++AA  F  RN LP  +QDQM++H+CL++RT  EGL+QQ+ ++ LPKAIR S
Sbjct: 326 RTRKFRDTIQAASGFGQRNNLPVRLQDQMVAHLCLRYRTDSEGLQQQEIIDSLPKAIRSS 385

Query: 302 IAHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSV 361
           I+HYLFY +V K YLF G+S+D LFQLV++++AEYFPPKEDVILQ+EA TD YILV+G+V
Sbjct: 386 ISHYLFYEVVDKIYLFHGISNDLLFQLVTEMKAEYFPPKEDVILQNEAPTDFYILVTGAV 445

Query: 362 DLISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSN 421
           D+I++++G +QV+  A  G  FGE GVLC +PQ  TVRT  LSQ+LR+ R  LL ++Q+N
Sbjct: 446 DIIARVNGVEQVVSEAQRGHVFGEVGVLCYRPQLFTVRTKRLSQLLRLNRTVLLNLVQAN 505

Query: 422 TDDGNIIMNNFFMKMKEYE 439
             DG IIMNN    +K+ E
Sbjct: 506 VGDGAIIMNNLLQHLKDSE 522

BLAST of Cp4.1LG20g05050 vs. TAIR 10
Match: AT4G32500.1 (K+ transporter 5 )

HSP 1 Score: 521.5 bits (1342), Expect = 8.7e-148
Identity = 255/437 (58.35%), Postives = 328/437 (75.06%), Query Frame = 0

Query: 2   WLVALVIYSAWICPFEFAFLPYKQNALFIIDHFVNAFFAVDIVLTFFVAYLDSQSYLLVD 61
           +LV LV+Y+AW  PFEF FL   +  L I+D+ VN FFAVDIVLTFFVA+LD  +YLLVD
Sbjct: 84  FLVILVLYTAWASPFEFGFLQTPRAPLSILDNVVNGFFAVDIVLTFFVAFLDKATYLLVD 143

Query: 62  HPKKIAVRYLSTWFIFDVCSTAPLQSISFLFTNQSGEVGFKLLNMLRLWRLRRVSSLFAR 121
            PK+IA RY STW IFDV ST P +    L  N     G+ + +MLRLWRL RVS  FAR
Sbjct: 144 DPKRIAWRYTSTWLIFDVVSTVPYELFGSLLHNTI--QGYGIFSMLRLWRLHRVSKCFAR 203

Query: 122 LEKDIRFNYFWTRCTKLISVTLFAVHCAGCFNFLIADKYPDPKRTWIGAVDPNFKEDSLW 181
           LEKD ++NYFW RCTKL+ V+LF VHC  CF + IA  YPDP  T++   + N+K+ SL 
Sbjct: 204 LEKDRKYNYFWIRCTKLLLVSLFVVHCGACFCYSIAAHYPDPSMTFMALAEANWKQKSLL 263

Query: 182 NLYITAIYWSITTLTTTGYGDLHAENPREMLFAIFYMLFNLGLTSYLIGNMTNLVVHWTS 241
             Y+TA+YWSITT +TTGYGD+H  N  E  F +FYM+FNLGL +Y+IGNMTNLVVH TS
Sbjct: 264 IRYVTAMYWSITTFSTTGYGDIHGNNAEERAFILFYMIFNLGLLAYIIGNMTNLVVHVTS 323

Query: 242 RTRNFRDSVRAAMEFASRNQLPNPIQDQMLSHICLKFRT--EGLKQQDTLNDLPKAIRVS 301
           RTRNFRD+++AA  FA RN LP  +Q+QM++H+ L++RT  EGL+QQ+ ++ LPKAIR S
Sbjct: 324 RTRNFRDTIQAASAFAQRNNLPLGLQEQMVAHLSLRYRTDSEGLQQQEIIDSLPKAIRSS 383

Query: 302 IAHYLFYPIVQKAYLFEGVSHDFLFQLVSDVEAEYFPPKEDVILQSEAQTDLYILVSGSV 361
           I+HYLFY +V K YLF G+S+D LFQLVS+++AEYFPPKEDVIL++EA +D YI+V+G+V
Sbjct: 384 ISHYLFYEVVDKTYLFHGISNDLLFQLVSEMKAEYFPPKEDVILRNEAPSDFYIMVTGAV 443

Query: 362 DLISKIDGHDQVIGRATGGDTFGEFGVLCQKPQPLTVRTTNLSQILRIKRASLLYIIQSN 421
           D+I++++G DQV+G A  G  FGE GVLC +PQ  TVRT  LSQ+LR+ R + L ++Q+N
Sbjct: 444 DIIARVNGVDQVVGEAQTGHVFGEVGVLCYRPQLFTVRTKRLSQLLRLNRTAFLNLVQAN 503

Query: 422 TDDGNIIMNNFFMKMKE 437
             DG IIMNN    +K+
Sbjct: 504 VGDGAIIMNNLLQHLKD 518