Cp4.1LG20g04260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g04260
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	TIR domain-containing protein
Location	Cp4.1LG20: 2406482 .. 2407135 (-)
RNA-Seq Expression	Cp4.1LG20g04260
Synteny	Cp4.1LG20g04260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATGCAACGTTATTTTTCATCTCTCCCCATAAACCATCTAATCCCCTCCACAATCCTCACTCGTCGCAACCACGTCCGATCCATGTCGCAGCGGTGCGACGTGTTCATAAACTACCGTGGTGTCGACACCCGACGGAGCATCGCTGGCTTGCTCTATGACCATTTTACTAGAATAGGGCTCCGCCCATTCTTAGACAGCGCCAACATGAAACCAGGAGACAAACTTTTTGGGGAGATTGAGGAAAGTATTAGGAGTTGTAAGGTGGGAATTGCGGTGTTTTCGCCGCGTTATTGTCAGTCTTATTTTTGTTTGCATGAACTTGCTTTAATGGTCGAGAATAAAAAGAAGATCATACCGATATTCGTCGACGTTCGGCCGTCTGAGCTTCGTGTCGAATTTAACCGAAACTGTCCCGAAAAAGAGATGGAGAGGTTCAATTGGGCACTTGGAGAAGCCAAATACACCGTTGGACTCACCTTCGACTCCATTAACGGGTAATTATTTCTCAATAAAATCGTGGCGTAATTTGTACCCAAAAGCACTCATGTTGTGTGATTATCTTGTTAGGGATTGGTCAGAGCTGCTCAGAAAAGCTTCAACCGCCGTCATTGATAATCTGGTCGCCGGAGAAGCGGTAGAGATGCCGGGAAAT

mRNA sequence

CCATGCAACGTTATTTTTCATCTCTCCCCATAAACCATCTAATCCCCTCCACAATCCTCACTCGTCGCAACCACGTCCGATCCATGTCGCAGCGGTGCGACGTGTTCATAAACTACCGTGGTGTCGACACCCGACGGAGCATCGCTGGCTTGCTCTATGACCATTTTACTAGAATAGGGCTCCGCCCATTCTTAGACAGCGCCAACATGAAACCAGGAGACAAACTTTTTGGGGAGATTGAGGAAAGTATTAGGAGTTGTAAGGTGGGAATTGCGGTGTTTTCGCCGCGTTATTGTCAGTCTTATTTTTGTTTGCATGAACTTGCTTTAATGGTCGAGAATAAAAAGAAGATCATACCGATATTCGTCGACGTTCGGCCGTCTGAGCTTCGTGTCGAATTTAACCGAAACTGTCCCGAAAAAGAGATGGAGAGGTTCAATTGGGCACTTGGAGAAGCCAAATACACCGTTGGACTCACCTTCGACTCCATTAACGGGGATTGGTCAGAGCTGCTCAGAAAAGCTTCAACCGCCGTCATTGATAATCTGGTCGCCGGAGAAGCGGTAGAGATGCCGGGAAAT

Coding sequence (CDS)

ATGCAACGTTATTTTTCATCTCTCCCCATAAACCATCTAATCCCCTCCACAATCCTCACTCGTCGCAACCACGTCCGATCCATGTCGCAGCGGTGCGACGTGTTCATAAACTACCGTGGTGTCGACACCCGACGGAGCATCGCTGGCTTGCTCTATGACCATTTTACTAGAATAGGGCTCCGCCCATTCTTAGACAGCGCCAACATGAAACCAGGAGACAAACTTTTTGGGGAGATTGAGGAAAGTATTAGGAGTTGTAAGGTGGGAATTGCGGTGTTTTCGCCGCGTTATTGTCAGTCTTATTTTTGTTTGCATGAACTTGCTTTAATGGTCGAGAATAAAAAGAAGATCATACCGATATTCGTCGACGTTCGGCCGTCTGAGCTTCGTGTCGAATTTAACCGAAACTGTCCCGAAAAAGAGATGGAGAGGTTCAATTGGGCACTTGGAGAAGCCAAATACACCGTTGGACTCACCTTCGACTCCATTAACGGGGATTGGTCAGAGCTGCTCAGAAAAGCTTCAACCGCCGTCATTGATAATCTGGTCGCCGGAGAAGCGGTAGAGATGCCGGGAAAT

Protein sequence

MQRYFSSLPINHLIPSTILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN

Homology

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BLAST of Cp4.1LG20g04260 vs. ExPASy Swiss-Prot
Match: I1GTC2 (TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.9e-40
Identity = 81/163 (49.69%), Postives = 116/163 (71.17%), Query Frame = 0


Query: 31  RCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGI 90
           R +VFIN+RGVDT+R++A LLYD   + GLR FLD+ +M+PGD+L   I  +IR C V +
Sbjct: 52  RYEVFINHRGVDTKRTVARLLYDRLAQAGLRGFLDNMSMRPGDRLEERIGSAIRECTVAV 111

Query: 91  AVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSEL----RVEFNRNCPEKEMERFN 150
           A+FSP YC S +CL ELA++VE++K IIPIF D++PS+L     V  +     +++ERF 
Sbjct: 112 AIFSPSYCDSEYCLRELAMLVESRKAIIPIFYDIKPSDLLLPQAVADSEVYLPRDLERFK 171

Query: 151 WALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVE 190
           +AL EAK+TVG+T+DS  GD +EL+  A+ AV+ N+   E V+
Sbjct: 172 FALREAKHTVGITYDSATGDMAELVSAAADAVMYNMEKMETVQ 214

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 35/102 (34.31%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 33  DVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAV 92
           DVF+++RG DTR++  G L++     G+  F+D   +K G  +  E+ ++I   +  + V
Sbjct: 13  DVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVV 72

Query: 93  FSPRYCQSYFCLHELALMVENKKK----IIPIFVDVRPSELR 131
           FS  Y  S +CL EL  ++E  +K    ++P+F DV PS +R
Sbjct: 73  FSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVR 114

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.7e-13
Identity = 38/102 (37.25%), Postives = 60/102 (58.82%), Query Frame = 0


Query: 33  DVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAV 92
           DVF+++RG DTR++    LY+     G++ F D   ++ G  + GE+ ++I   +  I V
Sbjct: 13  DVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVV 72

Query: 93  FSPRYCQSYFCLHELALMVENKKK----IIPIFVDVRPSELR 131
           FS  Y  S +CL+EL  ++E K +    +IPIF DV PS +R
Sbjct: 73  FSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVR 114

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy Swiss-Prot
Match: F4KIF3 (Disease resistance-like protein CSA1 OS=Arabidopsis thaliana OX=3702 GN=CSA1 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 45/142 (31.69%), Postives = 75/142 (52.82%), Query Frame = 0


Query: 34  VFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAVF 93
           VFIN+RGV+ R++    L     R G+  F+D+ + + G +L   + E I   ++ +A+F
Sbjct: 19  VFINFRGVELRKNFVSHLEKGLKRKGINAFIDT-DEEMGQEL-SVLLERIEGSRIALAIF 78

Query: 94  SPRYCQSYFCLHELALMVENKKK----IIPIFVDVRP---SELRVEFN-------RNCPE 153
           SPRY +S +CL ELA M E  ++    +IPIF  V+P    EL+ +F        ++  +
Sbjct: 79  SPRYTESKWCLKELAKMKERTEQKELVVIPIFYKVQPVTVKELKGDFGDKFRELVKSTDK 138

Query: 154 KEMERFNWALGEAKYTVGLTFD 162
           K  + +  AL    +  G+  D
Sbjct: 139 KTKKEWKEALQYVPFLTGIVLD 158

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy Swiss-Prot
Match: Q9SSN3 (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.1e-11
Identity = 42/148 (28.38%), Postives = 72/148 (48.65%), Query Frame = 0


Query: 33  DVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAV 92
           DVF+++RG DTR +    LY    R  +R F D   ++ G +   E++  I   +  + V
Sbjct: 10  DVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKELENGQRFSPELKSPIEVSRFAVVV 69

Query: 93  FSPRYCQSYFCLHELALMVENKKK----IIPIFVDVRPSELR-------VEFNRNCPEKE 152
            S  Y  S +CL EL  +++ +KK    ++PIF  V P+ +R        +F ++   ++
Sbjct: 70  VSENYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVRWQTGVLAEQFKKHASRED 129

Query: 153 MERFNWALGEAKYTVGLT-FDSINGDWS 169
            E+        K+   LT F  ++GD S
Sbjct: 130 PEKV------LKWRQALTNFAQLSGDCS 151

[Close]

BLAST of Cp4.1LG20g04260 vs. NCBI nr
Match: KAG7019631.1 (TMV resistance protein N, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390 bits (1003), Expect = 4.91e-137
Identity = 190/193 (98.45%), Postives = 191/193 (98.96%), Query Frame = 0


Query: 1   MQRYFSSLPINHLIPSTILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGL 60
           MQRYFSSLP NHL+PSTILTRRNHVRSMSQRCDVFINYRGVDTR SIAGLLYDHFTRIGL
Sbjct: 1   MQRYFSSLPRNHLVPSTILTRRNHVRSMSQRCDVFINYRGVDTRPSIAGLLYDHFTRIGL 60

Query: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120
           RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI
Sbjct: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120

Query: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID 180
           FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID
Sbjct: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID 180

Query: 181 NLVAGEAVEMPGN 193
           NLVAGEAVEMPGN
Sbjct: 181 NLVAGEAVEMPGN 193

[Close]

BLAST of Cp4.1LG20g04260 vs. NCBI nr
Match: KAG6584009.1 (TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 387 bits (995), Expect = 8.15e-136
Identity = 188/193 (97.41%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 1   MQRYFSSLPINHLIPSTILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGL 60
           MQRYFSSLP NHL+PSTILTRRNHV SMSQRCDVFINYRGVDTR SIAGLLYDHFTRIGL
Sbjct: 1   MQRYFSSLPRNHLVPSTILTRRNHVGSMSQRCDVFINYRGVDTRPSIAGLLYDHFTRIGL 60

Query: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120
           RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI
Sbjct: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120

Query: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID 180
           FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAV+D
Sbjct: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVVD 180

Query: 181 NLVAGEAVEMPGN 193
           NLVAGEAVEMPGN
Sbjct: 181 NLVAGEAVEMPGN 193

[Close]

BLAST of Cp4.1LG20g04260 vs. NCBI nr
Match: XP_023520219.1 (TMV resistance protein N-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343 bits (879), Expect = 1.53e-118
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 28  MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK 87
           MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK
Sbjct: 1   MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK 60

Query: 88  VGIAVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNW 147
           VGIAVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNW
Sbjct: 61  VGIAVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNW 120

Query: 148 ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 193
           ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN
Sbjct: 121 ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 166

[Close]

BLAST of Cp4.1LG20g04260 vs. NCBI nr
Match: XP_022927124.1 (toll/interleukin-1 receptor-like protein [Cucurbita moschata])

HSP 1 Score: 339 bits (870), Expect = 3.61e-117
Identity = 164/166 (98.80%), Postives = 165/166 (99.40%), Query Frame = 0


Query: 28  MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK 87
           MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK
Sbjct: 1   MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK 60

Query: 88  VGIAVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNW 147
           VGIAVFSPRYC+SYFCLHELALMVENKKKIIPIFVDVRPSEL VEFNRNCPEKEMERFNW
Sbjct: 61  VGIAVFSPRYCRSYFCLHELALMVENKKKIIPIFVDVRPSELHVEFNRNCPEKEMERFNW 120

Query: 148 ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 193
           ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN
Sbjct: 121 ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 166

[Close]

BLAST of Cp4.1LG20g04260 vs. NCBI nr
Match: XP_038893500.1 (TIR-only protein-like [Benincasa hispida])

HSP 1 Score: 330 bits (846), Expect = 4.23e-113
Identity = 155/193 (80.31%), Postives = 176/193 (91.19%), Query Frame = 0


Query: 1   MQRYFSSLPINHLIPSTILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGL 60
           MQRYFS++  N+L    IL+RRN +RS+S++CDVFIN+RGVDT+R+IAGLLYDHFTRIGL
Sbjct: 1   MQRYFSTVAKNYLTSCKILSRRNDIRSISRQCDVFINHRGVDTKRNIAGLLYDHFTRIGL 60

Query: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120
            PFLDS NMKPGDKLFGEIEE IRSCKVGIAVFSPRYC+SYFCLHELALMVENKKKIIPI
Sbjct: 61  HPFLDSKNMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALMVENKKKIIPI 120

Query: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID 180
           FVDVRPS+LRVE++ NCP+KE++RFNWALGEAKYTVGLTFDSINGDWSELLRKAS AV+D
Sbjct: 121 FVDVRPSQLRVEYSHNCPDKELQRFNWALGEAKYTVGLTFDSINGDWSELLRKASNAVVD 180

Query: 181 NLVAGEAVEMPGN 193
           NL+ G   +MP N
Sbjct: 181 NLIDGGGRKMPDN 193

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy TrEMBL
Match: A0A6J1EH47 (toll/interleukin-1 receptor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111434063 PE=4 SV=1)

HSP 1 Score: 339 bits (870), Expect = 1.75e-117
Identity = 164/166 (98.80%), Postives = 165/166 (99.40%), Query Frame = 0


Query: 28  MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK 87
           MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK
Sbjct: 1   MSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCK 60

Query: 88  VGIAVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNW 147
           VGIAVFSPRYC+SYFCLHELALMVENKKKIIPIFVDVRPSEL VEFNRNCPEKEMERFNW
Sbjct: 61  VGIAVFSPRYCRSYFCLHELALMVENKKKIIPIFVDVRPSELHVEFNRNCPEKEMERFNW 120

Query: 148 ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 193
           ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN
Sbjct: 121 ALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 166

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy TrEMBL
Match: A0A6J1KH70 (toll/interleukin-1 receptor-like protein OS=Cucurbita maxima OX=3661 GN=LOC111495714 PE=4 SV=1)

HSP 1 Score: 325 bits (832), Expect = 1.71e-111
Identity = 157/163 (96.32%), Postives = 158/163 (96.93%), Query Frame = 0


Query: 31  RCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGI 90
           RCDVFINYRGVDTR SIAGLLYDHF RIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGI
Sbjct: 17  RCDVFINYRGVDTRGSIAGLLYDHFARIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGI 76

Query: 91  AVFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNWALG 150
           AVFSPRYC+SYFCLHELALMVENKKKIIPIFVDVRPSEL VE NRNCPEKEMERFNWALG
Sbjct: 77  AVFSPRYCRSYFCLHELALMVENKKKIIPIFVDVRPSELHVELNRNCPEKEMERFNWALG 136

Query: 151 EAKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEAVEMPGN 193
           EAKYTVGLTFDS NGDWSELLRKASTAVIDNLVAGEAVEMPGN
Sbjct: 137 EAKYTVGLTFDSSNGDWSELLRKASTAVIDNLVAGEAVEMPGN 179

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy TrEMBL
Match: A0A5D3DRR9 (Disease resistance-like protein CSA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00490 PE=4 SV=1)

HSP 1 Score: 324 bits (830), Expect = 6.03e-111
Identity = 155/195 (79.49%), Postives = 181/195 (92.82%), Query Frame = 0


Query: 1   MQRYFSSLPINHLIPST-ILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIG 60
           MQRYFS++  N+L  S+ IL+RRN +RS+S++CDVFIN+RGVDT+R+IAGLL+DHF+RIG
Sbjct: 1   MQRYFSTVAKNYLTSSSKILSRRNDIRSLSRQCDVFINHRGVDTKRNIAGLLHDHFSRIG 60

Query: 61  LRPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIP 120
           LRPFLDS +MKPGDKLFGEIEE IRSCKVG+AVFSPRYC+SYFCLHELALMVENKKKIIP
Sbjct: 61  LRPFLDSKSMKPGDKLFGEIEEGIRSCKVGLAVFSPRYCESYFCLHELALMVENKKKIIP 120

Query: 121 IFVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVI 180
           IFVDVRPS+LRVE+N +CP+KE++RFNWALGEAKYTVGLTFD++NGDWSELLRKAS AVI
Sbjct: 121 IFVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVI 180

Query: 181 DNLVAGE-AVEMPGN 193
           DNL+ GE A EMP N
Sbjct: 181 DNLIVGEGAGEMPDN 195

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy TrEMBL
Match: A0A1S3BG53 (disease resistance-like protein CSA1 OS=Cucumis melo OX=3656 GN=LOC103489293 PE=4 SV=1)

HSP 1 Score: 324 bits (830), Expect = 6.03e-111
Identity = 155/195 (79.49%), Postives = 181/195 (92.82%), Query Frame = 0


Query: 1   MQRYFSSLPINHLIPST-ILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIG 60
           MQRYFS++  N+L  S+ IL+RRN +RS+S++CDVFIN+RGVDT+R+IAGLL+DHF+RIG
Sbjct: 1   MQRYFSTVAKNYLTSSSKILSRRNDIRSLSRQCDVFINHRGVDTKRNIAGLLHDHFSRIG 60

Query: 61  LRPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIP 120
           LRPFLDS +MKPGDKLFGEIEE IRSCKVG+AVFSPRYC+SYFCLHELALMVENKKKIIP
Sbjct: 61  LRPFLDSKSMKPGDKLFGEIEEGIRSCKVGLAVFSPRYCESYFCLHELALMVENKKKIIP 120

Query: 121 IFVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVI 180
           IFVDVRPS+LRVE+N +CP+KE++RFNWALGEAKYTVGLTFD++NGDWSELLRKAS AVI
Sbjct: 121 IFVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVI 180

Query: 181 DNLVAGE-AVEMPGN 193
           DNL+ GE A EMP N
Sbjct: 181 DNLIVGEGAGEMPDN 195

[Close]

BLAST of Cp4.1LG20g04260 vs. ExPASy TrEMBL
Match: A0A0A0LV24 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073070 PE=4 SV=1)

HSP 1 Score: 317 bits (812), Expect = 3.22e-108
Identity = 151/194 (77.84%), Postives = 177/194 (91.24%), Query Frame = 0


Query: 1   MQRYFSSLPINHLIPSTILTRRNHVRSMSQRCDVFINYRGVDTRRSIAGLLYDHFTRIGL 60
           MQRYFS++  ++L  S IL+RRN++RS+S++CDVFIN+RG DT+R+IAGLL+DHF+RIGL
Sbjct: 1   MQRYFSTVAKSYLTSSKILSRRNNIRSISRQCDVFINHRGADTKRNIAGLLHDHFSRIGL 60

Query: 61  RPFLDSANMKPGDKLFGEIEESIRSCKVGIAVFSPRYCQSYFCLHELALMVENKKKIIPI 120
             FLDS +MKPGDKLFGEIEE IRSCKVGIAVFSPRYC+SYFCLHELALM+ENKKKIIPI
Sbjct: 61  HSFLDSKSMKPGDKLFGEIEEGIRSCKVGIAVFSPRYCESYFCLHELALMMENKKKIIPI 120

Query: 121 FVDVRPSELRVEFNRNCPEKEMERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVID 180
           FVDVRPS+LRVE+N +CP+KE++RFNWALGEAKYTVGLTFD++NGDWSELLRKAS AVID
Sbjct: 121 FVDVRPSQLRVEYNYSCPKKELQRFNWALGEAKYTVGLTFDTVNGDWSELLRKASNAVID 180

Query: 181 NL-VAGEAVEMPGN 193
           NL V G A EMP N
Sbjct: 181 NLIVGGGAGEMPDN 194

[Close]

BLAST of Cp4.1LG20g04260 vs. TAIR 10
Match: AT1G61105.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 212.2 bits (539), Expect = 3.6e-55
Identity = 100/155 (64.52%), Postives = 125/155 (80.65%), Query Frame = 0


Query: 32  CDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIA 91
           CD+FIN+RG+DT+++I+GLLYD FTR  L  FLDS ++KPGD+LF EI+ +I++C VGIA
Sbjct: 29  CDIFINHRGIDTKKTISGLLYDCFTRQRLTAFLDSKSLKPGDRLFVEIDMAIKACGVGIA 88

Query: 92  VFSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNRNCPEKEMERFNWALGE 151
           VFSPRYC SYFCLHELAL+V+NKK++IPIF DV+PSEL V+ ++  P  E+ RF  AL E
Sbjct: 89  VFSPRYCDSYFCLHELALLVKNKKRVIPIFCDVKPSELCVKDDKTRPAAEIRRFQLALEE 148

Query: 152 AKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGE 187
           AKYTVGLTFD+ NGDWSE L  AS AV  NL+  E
Sbjct: 149 AKYTVGLTFDTSNGDWSEFLAMASDAVTKNLLEVE 183

[Close]

BLAST of Cp4.1LG20g04260 vs. TAIR 10
Match: AT1G52900.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 187.6 bits (475), Expect = 9.5e-48
Identity = 90/156 (57.69%), Postives = 115/156 (73.72%), Query Frame = 0


Query: 33  DVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAV 92
           DVFIN+RG DT+R+IA LLYD+     LRPFLDS NMKPGDKLF  I  +I + KV + V
Sbjct: 39  DVFINHRGSDTKRNIATLLYDNLKSRNLRPFLDSKNMKPGDKLFDHINNAILTSKVAVTV 98

Query: 93  FSPRYCQSYFCLHELALMVENKKKIIPIFVDVRPSELRVEFNR-NCPEKEMERFNWALGE 152
           FSP YC SYFCLHELAL++E++K++IPIF D++PS+L V   R  C + E++RF WAL E
Sbjct: 99  FSPNYCDSYFCLHELALIMESRKRVIPIFCDIKPSQLDVMIERVTCSDDEIQRFRWALQE 158

Query: 153 AKYTVGLTFDSINGDWSELLRKASTAVIDNLVAGEA 188
           AK  VGLTFDS  G+ SE++  AS  +++ LV  EA
Sbjct: 159 AKDIVGLTFDSYKGNLSEVVTVASDVIVERLVELEA 194

[Close]

BLAST of Cp4.1LG20g04260 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 83.2 bits (204), Expect = 2.5e-16
Identity = 49/162 (30.25%), Postives = 89/162 (54.94%), Query Frame = 0


Query: 31  RCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGI 90
           + DVF+++RG D R++    L+  F R+G++ F D  +++ G  +  E+ ++I+  +  I
Sbjct: 17  KTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELIDAIKGSRFAI 76

Query: 91  AVFSPRYCQSYFCLHELALMVE-NKKKIIPIFVDVRPSELR-------VEFNRNCPEKEM 150
            V S  Y  S +CL EL  ++E NK  I+PIF +V PS++R        +   +  ++++
Sbjct: 77  VVVSRNYAASSWCLDELLKIMECNKDTIVPIFYEVDPSDVRRQRGSFGEDVESHSDKEKV 136

Query: 151 ERFNWALGEAKYTVGLTFDSINGDWSELLRKASTAVIDNLVA 185
            ++  AL +     G   DS N D S+L++K    + D LV+
Sbjct: 137 GKWKEALKKLAAISG--EDSRNWDDSKLIKKIVKDISDKLVS 176

[Close]

BLAST of Cp4.1LG20g04260 vs. TAIR 10
Match: AT1G17615.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 79.3 bits (194), Expect = 3.7e-15
Identity = 34/105 (32.38%), Postives = 63/105 (60.00%), Query Frame = 0


Query: 30  QRCDVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVG 89
           Q+ DVF+++RG DTRR+    LY     +G+R F D   ++ G ++  ++  +I + K+ 
Sbjct: 13  QKFDVFLSFRGKDTRRTFISFLYKELIEMGIRTFKDDVELQSGRRIASDLLAAIENSKIA 72

Query: 90  IAVFSPRYCQSYFCLHELALMVENKKK----IIPIFVDVRPSELR 131
           + + S  Y  S +CL EL ++++ +KK    ++PIF +V P+ +R
Sbjct: 73  VVIISKNYSASPWCLQELVMIMDVEKKGSIIVMPIFYNVEPAHVR 117

[Close]

BLAST of Cp4.1LG20g04260 vs. TAIR 10
Match: AT5G48770.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-14
Identity = 38/102 (37.25%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 33  DVFINYRGVDTRRSIAGLLYDHFTRIGLRPFLDSANMKPGDKLFGEIEESIRSCKVGIAV 92
           DVF+++RG DTRR+I   LY      G+  F D  +++ GD +   +  +I   K  + V
Sbjct: 15  DVFLSFRGEDTRRTIVSHLYAALGAKGIITFKDDQDLEVGDHISSHLRRAIEGSKFAVVV 74

Query: 93  FSPRYCQSYFCLHELALMVE----NKKKIIPIFVDVRPSELR 131
            S RY  S +CL EL L++E     K K++P+F +V PS++R
Sbjct: 75  LSERYTTSRWCLMELQLIMELYNLGKLKVLPLFYEVDPSDVR 116

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g04260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1GTC2	1.9e-40	49.69	TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=...	[more]
A0A290U7C4	3.3e-13	34.31	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	9.7e-13	37.25	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
F4KIF3	3.1e-11	31.69	Disease resistance-like protein CSA1 OS=Arabidopsis thaliana OX=3702 GN=CSA1 PE=...	[more]
Q9SSN3	3.1e-11	28.38	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR ...	[more]

BLAST of Cp4.1LG20g04260 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019631.1	4.91e-137	98.45	TMV resistance protein N, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6584009.1	8.15e-136	97.41	TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023520219.1	1.53e-118	100.00	TMV resistance protein N-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927124.1	3.61e-117	98.80	toll/interleukin-1 receptor-like protein [Cucurbita moschata]	[more]
XP_038893500.1	4.23e-113	80.31	TIR-only protein-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG20g04260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EH47	1.75e-117	98.80	toll/interleukin-1 receptor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1KH70	1.71e-111	96.32	toll/interleukin-1 receptor-like protein OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A5D3DRR9	6.03e-111	79.49	Disease resistance-like protein CSA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BG53	6.03e-111	79.49	disease resistance-like protein CSA1 OS=Cucumis melo OX=3656 GN=LOC103489293 PE=...	[more]
A0A0A0LV24	3.22e-108	77.84	TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073070 PE=4 SV...	[more]

BLAST of Cp4.1LG20g04260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61105.1	3.6e-55	64.52	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G52900.1	9.5e-48	57.69	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT5G17680.1	2.5e-16	30.25	disease resistance protein (TIR-NBS-LRR class), putative	[more]
AT1G17615.1	3.7e-15	32.38	Disease resistance protein (TIR-NBS class)	[more]
AT5G48770.1	2.4e-14	37.25	Disease resistance protein (TIR-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 31..159 e-value: 2.9E-23 score: 93.3
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF13676	TIR_2	coord: 34..126 e-value: 9.3E-18 score: 65.0
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 30..175 score: 24.064095
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 14..151 e-value: 4.5E-32 score: 112.7
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 27..136
None	No IPR available	PANTHER	PTHR31008	COP1-INTERACTING PROTEIN-RELATED	coord: 26..185
None	No IPR available	PANTHER	PTHR31008:SF19	TOLL-INTERLEUKIN-RESISTANCE (TIR) DOMAIN FAMILY PROTEIN	coord: 26..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g04260.1	Cp4.1LG20g04260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity