Cp4.1LG20g04200 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g04200
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionSphingolipid delta(4)-desaturase DES1-like
LocationCp4.1LG20: 2461484 .. 2463802 (+)
RNA-Seq ExpressionCp4.1LG20g04200
SyntenyCp4.1LG20g04200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGAGGAATCCATTGTCCACAGTGGCAAGTTGGGAGGCATAGGAAGATGGGTACAGCCGGAATACATCAGAGTCCACTCGAACGAAACGCCCTTCTCTAAATATAGCAGTCTCCTCCATTTCCATTTTCATATTTGGTCGGTCTTCTTCAATTCAATTCCAGGCCGTTCCTCTGCAACCAGTTTCCTGTATAAGTAACCGTTTCTTTCTCCTCCTTAATCCACAGCCAATTTCAACGCCAGGAGCCGCCAGGAGAGACAGAATCCGCCCAGGCATATTCCGGGAGCGAAGGTCAGCCGCAAGCAGAGGCAATGCGAGGAGGTGAAGAGAGAGATGAGGGTGTAATGGCCACTGACTTCTTCTGGTCATACACCGACGAGCCTCACGCTTCAAGGAGACGCCAAATTCTCTCGCAGTACCCTCAGATTCGGGAGCTCTTCGGTCCTGACCCGTGGGCTTGCTTCAAGGTTTCATTTGTTTCTCTATGATTTTACTGTTGTTGGTTCTGAGAATGTGGAGAGTCCTTGTCATTTTCTTGTCAAGATTTTTTGATTTCGTGTGTAGATCTGAATTGAGGTATTGCTGGTCGCACCACATGTCAATGGCTGAATCTGATCGGTGTTTGTTTGTTGTTCTGTTTGATTTTGAACGCCACCTCTGCCACATTGCTGCATTGGACATTGTTTTTCATTTTCTATTCACCACCAGATTACATCTCGCAAGTTGTATTGATCGAAAATCTACATTCTGAGATTCTGAAATTTTGTCGTTCTTATTGCTCTGGTTCAGTTTTTCTGCTAGATTCCTGTATTCTATCTTTCCCCCTTTTTTCCATCTATCGCTTGTTTGTTTGCTTTTTCCTTCCTTGAACATTTGTCTGAACCTTCCGATTGTTTAGGTCTAAAATTGATTTAGACCTAAACAATCATTATTTTGAACTTTCCTGTGTTGTTTGCTCAACGAATCATAATTTGGATTCATCTGATGAAGAAAGTGAAGTTGCTAAGAATGTGAACAACCATTTTACACATTCACAAGTGACCTTGTAGTCTTAGAAATTAGCTATTGGAGTTTGAAAAACCAATCAATGATCTGTTCTTGTAATATAAAGTAATCCATTTGTTCTTCTCATTCTTGCAATCTTGCAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCCACGACCCTTCACAATGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTGAACCACAATCTGTTCTTAGCTATTCACGAGCTCAGCCACAACCTTGCCTTCTCAACACCTGATCACAACAGGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAGAAGTATCACCTAGAGCACCATCGCTTCCAGGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACTGAAGCTCGTCTGGTGACTAATGTCATCTCAAAAGCTATATGGGTTGTCCTCCAACTCTTCTTCTATGCTCTCCGGCCATTGTTTCTAAAACCAAAGCCCCCAGGCCGGTGGGAGTTCATCAACTTCTTTATTCAGATAACTCTTGATGTTGCCATGGTTTACTTTTGGAGCTGGAAGTCTTTTGCTTACCTGATTCTCTCCACCTTCGTCGGTGGCGGGATGCATCCGATGGCTGGTCACTTCATTTCCGAACATTACGTATTCAATCCCAAACAAGAGACGTACTCTTACTACGGTCCTCTTAATCTTCTTACATGGAGTGTGGGTTACCATAATGAGCACCATGACTTCCCCAGGATCCCAGGAAACAAGCTTTACAAGGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCGTATAAATCATGGAGCCAGGTGATATACATGTACATTATGGACAGGTCAGTGGGGCCATTCAGTAGGATGAAGAGACTGTCAAAGAAACCCGAGTAGTCCATCCTTTTCTCTCCCCCTTTATATTCTTTTGTTTCATTCTTTTTCCATCTTTCTCTCTTGAGCTGGTTTTGCCATTGTGTTTTTGCCTACGTTCGTGGAGAGTTTATTTGTTCTACTTAGATTGTATAATAGCCGGGAATGTATGTTGACTGTACTGTAATCCATTTTCAGAGCTTTTTGAGGAACTGATTGTAAAAGGAGCCGAACATTGGCGAACTTCGATCCTCATAGGCTTGGTAATTGGTTTGCTTACAACTATACTTTCAGGGCTGTGCCTCCATATTTTCATCTTAATGTCTAAATTCACTATCTTATCTAATCTTCATTCATGAACCATTTTATCCCTTTCT

mRNA sequence

TAGAGGAATCCATTGTCCACAGTGGCAAGTTGGGAGGCATAGGAAGATGGGTACAGCCGGAATACATCAGAGTCCACTCGAACGAAACGCCCTTCTCTAAATATAGCAGTCTCCTCCATTTCCATTTTCATATTTGCCAATTTCAACGCCAGGAGCCGCCAGGAGAGACAGAATCCGCCCAGGCATATTCCGGGAGCGAAGGTCAGCCGCAAGCAGAGGCAATGCGAGGAGGTGAAGAGAGAGATGAGGGTGTAATGGCCACTGACTTCTTCTGGTCATACACCGACGAGCCTCACGCTTCAAGGAGACGCCAAATTCTCTCGCAGTACCCTCAGATTCGGGAGCTCTTCGGTCCTGACCCGTGGGCTTGCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCCACGACCCTTCACAATGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTGAACCACAATCTGTTCTTAGCTATTCACGAGCTCAGCCACAACCTTGCCTTCTCAACACCTGATCACAACAGGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAGAAGTATCACCTAGAGCACCATCGCTTCCAGGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACTGAAGCTCGTCTGGTGACTAATGTCATCTCAAAAGCTATATGGGTTGTCCTCCAACTCTTCTTCTATGCTCTCCGGCCATTGTTTCTAAAACCAAAGCCCCCAGGCCGGTGGGAGTTCATCAACTTCTTTATTCAGATAACTCTTGATGTTGCCATGGTTTACTTTTGGAGCTGGAAGTCTTTTGCTTACCTGATTCTCTCCACCTTCGTCGGTGGCGGGATGCATCCGATGGCTGGTCACTTCATTTCCGAACATTACGTATTCAATCCCAAACAAGAGACGTACTCTTACTACGGTCCTCTTAATCTTCTTACATGGAGTGTGGGTTACCATAATGAGCACCATGACTTCCCCAGGATCCCAGGAAACAAGCTTTACAAGGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCGTATAAATCATGGAGCCAGGTGATATACATGTACATTATGGACAGGTCAGTGGGGCCATTCAGTAGGATGAAGAGACTGTCAAAGAAACCCGAGTAGTCCATCCTTTTCTCTCCCCCTTTATATTCTTTTGTTTCATTCTTTTTCCATCTTTCTCTCTTGAGCTGGTTTTGCCATTGTGTTTTTGCCTACGTTCGTGGAGAGTTTATTTGTTCTACTTAGATTGTATAATAGCCGGGAATGTATGTTGACTGTACTGTAATCCATTTTCAGAGCTTTTTGAGGAACTGATTGTAAAAGGAGCCGAACATTGGCGAACTTCGATCCTCATAGGCTTGGTAATTGGTTTGCTTACAACTATACTTTCAGGGCTGTGCCTCCATATTTTCATCTTAATGTCTAAATTCACTATCTTATCTAATCTTCATTCATGAACCATTTTATCCCTTTCT

Coding sequence (CDS)

ATGCGAGGAGGTGAAGAGAGAGATGAGGGTGTAATGGCCACTGACTTCTTCTGGTCATACACCGACGAGCCTCACGCTTCAAGGAGACGCCAAATTCTCTCGCAGTACCCTCAGATTCGGGAGCTCTTCGGTCCTGACCCGTGGGCTTGCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACTGCCACGACCCTTCACAATGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTGAACCACAATCTGTTCTTAGCTATTCACGAGCTCAGCCACAACCTTGCCTTCTCAACACCTGATCACAACAGGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAGAAGTATCACCTAGAGCACCATCGCTTCCAGGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACTGAAGCTCGTCTGGTGACTAATGTCATCTCAAAAGCTATATGGGTTGTCCTCCAACTCTTCTTCTATGCTCTCCGGCCATTGTTTCTAAAACCAAAGCCCCCAGGCCGGTGGGAGTTCATCAACTTCTTTATTCAGATAACTCTTGATGTTGCCATGGTTTACTTTTGGAGCTGGAAGTCTTTTGCTTACCTGATTCTCTCCACCTTCGTCGGTGGCGGGATGCATCCGATGGCTGGTCACTTCATTTCCGAACATTACGTATTCAATCCCAAACAAGAGACGTACTCTTACTACGGTCCTCTTAATCTTCTTACATGGAGTGTGGGTTACCATAATGAGCACCATGACTTCCCCAGGATCCCAGGAAACAAGCTTTACAAGGTGAAGGAGATTGCACCTGAATACTACAATGAATTAGATTCGTATAAATCATGGAGCCAGGTGATATACATGTACATTATGGACAGGTCAGTGGGGCCATTCAGTAGGATGAAGAGACTGTCAAAGAAACCCGAGTAG

Protein sequence

MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDRSVGPFSRMKRLSKKPE
Homology
[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy Swiss-Prot
Match: Q9ZPH4 (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.0e-160
Identity = 266/320 (83.12%), Postives = 289/320 (90.31%), Query Frame = 0 

Query: 5   EERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLWTA 64
           EE  EGVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWA  KI++VV+LQL TA
Sbjct: 13  EEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTA 72

Query: 65  TTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPM 124
             LHN+ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP +NR LGIFANLPIGVPM
Sbjct: 73  AILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPM 132

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMDVP+ TEA LVTN+ +K IWV LQLFFYALRP+F+KPK
Sbjct: 133 SVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPK 192

Query: 185 PPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG WEFINF IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP Q
Sbjct: 193 PPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ 252

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKL+ VKEIA EYY  L+SYKSWSQVIYMY
Sbjct: 253 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMY 312

Query: 305 IMDRSVGPFSRMKRLSKKPE 325
           IMD +VGP+SRMKR   K +
Sbjct: 313 IMDTTVGPYSRMKRKLSKSD 332

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy Swiss-Prot
Match: Q6H5U3 (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 5.2e-160
Identity = 259/322 (80.43%), Postives = 286/322 (88.82%), Query Frame = 0 

Query: 3   GGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLW 62
           G    +EGVMATDFFWSYTDEPHA+RRR+IL+++PQI+ELFGPDP A  KI+ VV LQLW
Sbjct: 6   GDGREEEGVMATDFFWSYTDEPHATRRREILAKHPQIKELFGPDPLAFLKIAAVVSLQLW 65

Query: 63  TATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGV 122
           TAT L +A W+KIL +AYFFGSFLNHNLFLAIHELSHNLAF+TP +NRWLGIFANLPIGV
Sbjct: 66  TATLLRDASWVKILTVAYFFGSFLNHNLFLAIHELSHNLAFTTPSYNRWLGIFANLPIGV 125

Query: 123 PMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLK 182
           PMSITFQKYHLEHHRFQGVDG+DMD+PSQ EA  V N +SK++WVV QLFFYALRPLFLK
Sbjct: 126 PMSITFQKYHLEHHRFQGVDGIDMDIPSQAEAHAVKNTLSKSVWVVFQLFFYALRPLFLK 185

Query: 183 PKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 242
           PKPPG WEF N  IQI LD +MVYF+ WKS AYLILSTFVGGGMHPMAGHFISEHYVFNP
Sbjct: 186 PKPPGLWEFTNLIIQIALDASMVYFFGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 245

Query: 243 KQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIY 302
            QETYSYYGPLNL+TW VGYHNEHHDFPRIPG +LYKV+EIAPEYYN L SYKSWSQVIY
Sbjct: 246 DQETYSYYGPLNLMTWHVGYHNEHHDFPRIPGTRLYKVREIAPEYYNNLKSYKSWSQVIY 305

Query: 303 MYIMDRSVGPFSRMKRLSKKPE 325
           MYIMD++VGPFSRMKR + K +
Sbjct: 306 MYIMDQTVGPFSRMKRKAPKKD 327

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy Swiss-Prot
Match: O15121 (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.7e-94
Identity = 160/313 (51.12%), Postives = 217/313 (69.33%), Query Frame = 0 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLWTATTLHNA 70
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP   + I ++VL QL     + + 
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFST--PDHNRWLGIFANLPIGVPMSITF 130
           DW  ++  AY FGS +NH++ LAIHE++HN AF       NRW G+FANLPIG+P SI+F
Sbjct: 65  DWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLKPKPPGR 190
           ++YH++HHR+ G DG+D+D+P+  E         K IWV+LQ  FYA RPLF+ PKP   
Sbjct: 125 KRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184

Query: 191 WEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN   Q+T D+ + YF   KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDR 310
           YYGPLNLLT++VGYHNEHHDFP IPG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDD 304

Query: 311 SVGPFSRMKRLSK 322
           ++ P+SRMKR  K
Sbjct: 305 TISPYSRMKRHQK 317

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy Swiss-Prot
Match: Q6UQ04 (Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.0e-94
Identity = 158/316 (50.00%), Postives = 219/316 (69.30%), Query Frame = 0 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLWTATTLHNA 70
           V   +F W YTD+PH  RRR+IL++YP+I+ L  PDP   + I+ + L+QL +   + + 
Sbjct: 5   VSREEFEWVYTDQPHTDRRREILAKYPEIKSLMKPDPNLIWIITAMFLVQLISLYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDHNRWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F       NRW GIFANLPIGVP S++F
Sbjct: 65  DWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKAVWNRWFGIFANLPIGVPYSVSF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLKPKPPGR 190
           ++YH++HHR+ G DG+D+D+P+  E         K +WV+LQ  FYALRPLF+ PKP   
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTFRKLVWVILQPLFYALRPLFINPKPITY 184

Query: 191 WEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN   QI LD+ + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEIINTVTQIILDIIVYYVFGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDR 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY++L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIAAEYYDKLPQYNSWIKVLYDFVMDD 304

Query: 311 SVGPFSRMKRLSKKPE 325
           ++ P+SRMKR  K  E
Sbjct: 305 TISPYSRMKRPPKGSE 320

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy Swiss-Prot
Match: Q5RE51 (Sphingolipid delta(4)-desaturase DES1 OS=Pongo abelii OX=9601 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.4e-93
Identity = 158/313 (50.48%), Postives = 215/313 (68.69%), Query Frame = 0 

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLWTATTLHNA 70
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP   + I ++VL QL     + + 
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLAAFYIVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFST--PDHNRWLGIFANLPIGVPMSITF 130
           DW  ++  AY FGS +NH++ L IHE++HN AF       NRW G+FANLPIG+P S++F
Sbjct: 65  DWKWVIFGAYAFGSCINHSMTLGIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSVSF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLKPKPPGR 190
           + YH++HHR+ G DG+D+D+P+  E         K IWV+LQ  FYA RPLF+ PKP   
Sbjct: 125 KSYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184

Query: 191 WEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN   Q+T D+ + YF   KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDR 310
           YYGPLNLLT++VGYHNEHHDFP IPG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDD 304

Query: 311 SVGPFSRMKRLSK 322
           ++ P+SRMKR  K
Sbjct: 305 TISPYSRMKRHQK 317

[Close]
BLAST of Cp4.1LG20g04200 vs. NCBI nr
Match: XP_023519136.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 683 bits (1763), Expect = 1.64e-248
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ
Sbjct: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMDRSVGPFSRMKRLSKKPE
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. NCBI nr
Match: XP_022927006.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >XP_022927007.1 sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG7019623.1 Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 677 bits (1748), Expect = 3.19e-246
Identity = 322/324 (99.38%), Postives = 323/324 (99.69%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLHNA+WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNAEWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMDRSVGPFSRMKRLSKKPE
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. NCBI nr
Match: XP_023001733.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita maxima])

HSP 1 Score: 674 bits (1738), Expect = 1.07e-244
Identity = 319/324 (98.46%), Postives = 322/324 (99.38%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           MRGG ERDEGV+ATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MRGGGERDEGVIATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWALFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNV+SKAIWVVLQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVVSKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGRWEFINFFIQITLDVAMVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMDRSVGPFSRMKRLSKKPE
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. NCBI nr
Match: XP_038893930.1 (sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida])

HSP 1 Score: 649 bits (1673), Expect = 8.67e-235
Identity = 306/324 (94.44%), Postives = 315/324 (97.22%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           M+GGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MQGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH+ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+NRWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTNV+SKAIWVV QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSLTEAHLVTNVVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG+WEFINF IQI LDVAMVY WSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGQWEFINFIIQIALDVAMVYLWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMDR+VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDRTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. NCBI nr
Match: XP_004146595.1 (sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothetical protein Csa_014335 [Cucumis sativus])

HSP 1 Score: 648 bits (1672), Expect = 1.23e-234
Identity = 304/324 (93.83%), Postives = 315/324 (97.22%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           M GGEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH+ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy TrEMBL
Match: A0A6J1EFX3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111433967 PE=3 SV=1)

HSP 1 Score: 677 bits (1748), Expect = 1.54e-246
Identity = 322/324 (99.38%), Postives = 323/324 (99.69%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLHNA+WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNAEWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMDRSVGPFSRMKRLSKKPE
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy TrEMBL
Match: A0A6J1KRE0 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita maxima OX=3661 GN=LOC111495782 PE=3 SV=1)

HSP 1 Score: 674 bits (1738), Expect = 5.16e-245
Identity = 319/324 (98.46%), Postives = 322/324 (99.38%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           MRGG ERDEGV+ATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MRGGGERDEGVIATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWALFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNV+SKAIWVVLQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVVSKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGRWEFINFFIQITLDVAMVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMDRSVGPFSRMKRLSKKPE
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy TrEMBL
Match: A0A0A0LSB9 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G073750 PE=3 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 5.97e-235
Identity = 304/324 (93.83%), Postives = 315/324 (97.22%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           M GGEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWA FKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH+ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy TrEMBL
Match: A0A5D3DSS5 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00680 PE=3 SV=1)

HSP 1 Score: 644 bits (1662), Expect = 2.00e-233
Identity = 301/324 (92.90%), Postives = 315/324 (97.22%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           M+GG ERD+GVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWA FKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH+ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. ExPASy TrEMBL
Match: A0A1S3B5L3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC103486276 PE=3 SV=1)

HSP 1 Score: 644 bits (1662), Expect = 2.00e-233
Identity = 301/324 (92.90%), Postives = 315/324 (97.22%), Query Frame = 0 

Query: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQ 60
           M+GG ERD+GVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWA FKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH+ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

[Close]
BLAST of Cp4.1LG20g04200 vs. TAIR 10
Match: AT4G04930.1 (fatty acid desaturase family protein )

HSP 1 Score: 567.4 bits (1461), Expect = 7.4e-162
Identity = 266/320 (83.12%), Postives = 289/320 (90.31%), Query Frame = 0 

Query: 5   EERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWACFKISVVVLLQLWTA 64
           EE  EGVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWA  KI++VV+LQL TA
Sbjct: 13  EEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQLSTA 72

Query: 65  TTLHNADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPM 124
             LHN+ WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP +NR LGIFANLPIGVPM
Sbjct: 73  AILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPIGVPM 132

Query: 125 SITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLFLKPK 184
           S+TFQKYHLEHHRFQGVDG+DMDVP+ TEA LVTN+ +K IWV LQLFFYALRP+F+KPK
Sbjct: 133 SVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIFIKPK 192

Query: 185 PPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQ 244
           PPG WEFINF IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP Q
Sbjct: 193 PPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQ 252

Query: 245 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQVIYMY 304
           ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKL+ VKEIA EYY  L+SYKSWSQVIYMY
Sbjct: 253 ETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQVIYMY 312

Query: 305 IMDRSVGPFSRMKRLSKKPE 325
           IMD +VGP+SRMKR   K +
Sbjct: 313 IMDTTVGPYSRMKRKLSKSD 332

The following BLAST results are available for this feature:
BLAST of Cp4.1LG20g04200 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZPH41.0e-16083.13Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q6H5U35.2e-16080.43Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39... [more]
O151211.7e-9451.12Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1[more]
Q6UQ045.0e-9450.00Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2... [more]
Q5RE511.4e-9350.48Sphingolipid delta(4)-desaturase DES1 OS=Pongo abelii OX=9601 GN=DEGS1 PE=2 SV=1[more]
BLAST of Cp4.1LG20g04200 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023519136.11.64e-248100.00sphingolipid delta(4)-desaturase DES1-like [Cucurbita pepo subsp. pepo][more]
XP_022927006.13.19e-24699.38sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >XP_022927007.1 ... [more]
XP_023001733.11.07e-24498.46sphingolipid delta(4)-desaturase DES1-like [Cucurbita maxima][more]
XP_038893930.18.67e-23594.44sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida][more]
XP_004146595.11.23e-23493.83sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothe... [more]
BLAST of Cp4.1LG20g04200 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EFX31.54e-24699.38Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KRE05.16e-24598.46Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LSB95.97e-23593.83Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G0... [more]
A0A5D3DSS52.00e-23392.90Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B5L32.00e-23392.90Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC1034862... [more]
BLAST of Cp4.1LG20g04200 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G04930.17.4e-16283.13fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013866Sphingolipid delta4-desaturase, N-terminalSMARTSM01269Lipid_DES_2coord: 11..49
e-value: 7.2E-21
score: 85.3
IPR013866Sphingolipid delta4-desaturase, N-terminalPFAMPF08557Lipid_DEScoord: 14..48
e-value: 6.7E-20
score: 70.3
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 72..290
e-value: 7.5E-23
score: 81.7
IPR011388Sphingolipid delta4-desaturasePIRSFPIRSF017228Sphnglp_dlt4_descoord: 4..324
e-value: 2.2E-149
score: 495.0
IPR011388Sphingolipid delta4-desaturaseCDDcd03508Delta4-sphingolipid-FADS-likecoord: 32..318
e-value: 7.20911E-171
score: 474.055
NoneNo IPR availablePANTHERPTHR12879:SF17SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKEcoord: 5..322
NoneNo IPR availablePANTHERPTHR12879SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2coord: 5..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g04200.1Cp4.1LG20g04200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046513 ceramide biosynthetic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0030148 sphingolipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042284 sphingolipid delta-4 desaturase activity