Cp4.1LG20g03710 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g03710
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	gibberellin 2-beta-dioxygenase 8
Location	Cp4.1LG20: 2164518 .. 2166018 (+)
RNA-Seq Expression	Cp4.1LG20g03710
Synteny	Cp4.1LG20g03710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTATAATAATATTATTTACATAAAATGGGTTGTTTGGAAGGTACAGACTATATGTTAAAATAAATATTAATTTTGGAGGGTGAAAATATTCTATATAAATCTAAACATTACAAACCAAGAGCATCAACAACAAAGCTTTCCTTTTAAATCGTCGTAATTTTTTTGGGATAATTATATCAGTCTGCCAACGTGGCTATGGACCCACCATTTCACGAGGCTTACAAGAGTCTGTTGGCGGAAACGGCGGTGGATAGAGGAGGCGACGCTGTGGCGGTGAAGGAATGGGAGCTTCCGGTGGTTGATTTGAGGCGTTTGAGGGACGGAGCGGAGGTCGGGGAAGTGGAACAGTGCAAAAATGATATAATTAGAGCTTCTCAGGAATGGGGGTTTTTTCAAGTTGTGAATCATGGGGTTTCGAGTGAGCTTTTGGGTAAGATGAGAGCCAAACAAATTGAGCTTTTTAAACAGCCGTTTGAGCGTAAGAGTAAGGAGGACATGTTCTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACACCCACCGCCACTTCTCTTACCCAACTCTCTTGGTCGGAAGCCTTCCATGTTTCTCTCTCCGATATTCTTGGATCCAACGGCTCCGATGATCTCAGGTTCCTTCCAAACATGCAAATCCATGCATTATTCCAAATTATTAAAAATTATATTTAAATAATTATATTCTGGTGTTAAATATTAAGCAGGTCAACAATGGAAGAATTCGCGGGGAAAGTTTCGAGGTTGGCGCAAGAGCTAGCGGAGATTTTGGGAGAGAATTTGGGGAGAAGCTCTAAATTCTTCGTTGACAATTGTGTGCCAAGCACGTGCTATCTCCGGATGAACCGGTACCCGCCATGCTCGGTTCCGGGTCGGGTATTCGGACTCATGCCTCACACAGACAGCGATTTTTTGACCATTCTTCACCAAGACCAAGTCGGTGGCTTGGAGTTGGTCAAGGACGGGGAGTGGATCGCCGTTAAACCCAACCCCGAGGCTCTTATAATCAACATTGGCGACTTGTTTCAGGTCAAATATATTTATTATTATTATTATTATTTTATTAATGGAATAATTATAATAATTTTAAACTTAATCAACTATAGGCTTGGAGCAACGATGTTTACAAGAGTGTCGAACATAGAGTTGTGACGAACTCGAAGTTGGAGAGATTTTCGATAGCTTATTTCTTGTGTCCTTGGAGCGAGACGGTTATAAAAAGCAAATGCGAGCCTGGGGTTTATAGAAGGTTTAGTTTTAGAGAATTTCGGAATCAGGTTCAAGAGGATGTTCGTAAGCATGGTTTCAAGATTGGGTTGCCCAAATTTGTCTTATGACGAAACAAAAAGACAATAAATGTGTTTTCTTTTTAAATAAATAAGTTATAAAGTAAAATCGATACTGGTAATCGTAGTTTTGTGATGTACATCGAGATATATCAATCTTACTCTAGAGAAGAAATGACAAATATTGTTCAAATTACT

mRNA sequence

AATTATAATAATATTATTTACATAAAATGGGTTGTTTGGAAGGTACAGACTATATGTTAAAATAAATATTAATTTTGGAGGGTGAAAATATTCTATATAAATCTAAACATTACAAACCAAGAGCATCAACAACAAAGCTTTCCTTTTAAATCGTCGTAATTTTTTTGGGATAATTATATCAGTCTGCCAACGTGGCTATGGACCCACCATTTCACGAGGCTTACAAGAGTCTGTTGGCGGAAACGGCGGTGGATAGAGGAGGCGACGCTGTGGCGGTGAAGGAATGGGAGCTTCCGGTGGTTGATTTGAGGCGTTTGAGGGACGGAGCGGAGGTCGGGGAAGTGGAACAGTGCAAAAATGATATAATTAGAGCTTCTCAGGAATGGGGGTTTTTTCAAGTTGTGAATCATGGGGTTTCGAGTGAGCTTTTGGGTAAGATGAGAGCCAAACAAATTGAGCTTTTTAAACAGCCGTTTGAGCGTAAGAGTAAGGAGGACATGTTCTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACACCCACCGCCACTTCTCTTACCCAACTCTCTTGGTCGGAAGCCTTCCATGTTTCTCTCTCCGATATTCTTGGATCCAACGGCTCCGATGATCTCAGGTCAACAATGGAAGAATTCGCGGGGAAAGTTTCGAGGTTGGCGCAAGAGCTAGCGGAGATTTTGGGAGAGAATTTGGGGAGAAGCTCTAAATTCTTCGTTGACAATTGTGTGCCAAGCACGTGCTATCTCCGGATGAACCGGTACCCGCCATGCTCGGTTCCGGGTCGGGTATTCGGACTCATGCCTCACACAGACAGCGATTTTTTGACCATTCTTCACCAAGACCAAGTCGGTGGCTTGGAGTTGGTCAAGGACGGGGAGTGGATCGCCGTTAAACCCAACCCCGAGGCTCTTATAATCAACATTGGCGACTTGTTTCAGGCTTGGAGCAACGATGTTTACAAGAGTGTCGAACATAGAGTTGTGACGAACTCGAAGTTGGAGAGATTTTCGATAGCTTATTTCTTGTGTCCTTGGAGCGAGACGGTTATAAAAAGCAAATGCGAGCCTGGGGTTTATAGAAGGTTTAGTTTTAGAGAATTTCGGAATCAGGTTCAAGAGGATGTTCGTAAGCATGGTTTCAAGATTGGGTTGCCCAAATTTGTCTTATGACGAAACAAAAAGACAATAAATGTGTTTTCTTTTTAAATAAATAAGTTATAAAGTAAAATCGATACTGGTAATCGTAGTTTTGTGATGTACATCGAGATATATCAATCTTACTCTAGAGAAGAAATGACAAATATTGTTCAAATTACT

Coding sequence (CDS)

ATGGACCCACCATTTCACGAGGCTTACAAGAGTCTGTTGGCGGAAACGGCGGTGGATAGAGGAGGCGACGCTGTGGCGGTGAAGGAATGGGAGCTTCCGGTGGTTGATTTGAGGCGTTTGAGGGACGGAGCGGAGGTCGGGGAAGTGGAACAGTGCAAAAATGATATAATTAGAGCTTCTCAGGAATGGGGGTTTTTTCAAGTTGTGAATCATGGGGTTTCGAGTGAGCTTTTGGGTAAGATGAGAGCCAAACAAATTGAGCTTTTTAAACAGCCGTTTGAGCGTAAGAGTAAGGAGGACATGTTCTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACACCCACCGCCACTTCTCTTACCCAACTCTCTTGGTCGGAAGCCTTCCATGTTTCTCTCTCCGATATTCTTGGATCCAACGGCTCCGATGATCTCAGGTCAACAATGGAAGAATTCGCGGGGAAAGTTTCGAGGTTGGCGCAAGAGCTAGCGGAGATTTTGGGAGAGAATTTGGGGAGAAGCTCTAAATTCTTCGTTGACAATTGTGTGCCAAGCACGTGCTATCTCCGGATGAACCGGTACCCGCCATGCTCGGTTCCGGGTCGGGTATTCGGACTCATGCCTCACACAGACAGCGATTTTTTGACCATTCTTCACCAAGACCAAGTCGGTGGCTTGGAGTTGGTCAAGGACGGGGAGTGGATCGCCGTTAAACCCAACCCCGAGGCTCTTATAATCAACATTGGCGACTTGTTTCAGGCTTGGAGCAACGATGTTTACAAGAGTGTCGAACATAGAGTTGTGACGAACTCGAAGTTGGAGAGATTTTCGATAGCTTATTTCTTGTGTCCTTGGAGCGAGACGGTTATAAAAAGCAAATGCGAGCCTGGGGTTTATAGAAGGTTTAGTTTTAGAGAATTTCGGAATCAGGTTCAAGAGGATGTTCGTAAGCATGGTTTCAAGATTGGGTTGCCCAAATTTGTCTTATGA

Protein sequence

MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL

Homology

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 429.9 bits (1104), Expect = 2.6e-119
Identity = 207/338 (61.24%), Postives = 254/338 (75.15%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGD----------AVAVKEWELPVVDLRRLRDGAEVGEVE 60
           MDPPF+E Y +LL      +  D             V+E ELPV+D+ RL DGAE  E E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAE-EERE 60

Query: 61  QCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSY 120
           +CK  I RAS+EWGFFQV+NHG+S ++L KMR +QI +F++PF++KSK +    FSAGSY
Sbjct: 61  KCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSE---KFSAGSY 120

Query: 121 RWGTPTATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGEN 180
           RWGTP+ATS+ QLSWSEAFHV ++DI  +     L STME+FA +   LA  LAE+L E 
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEK 180

Query: 181 LGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVK 240
            G++S FF +NCV +TCYLRMNRYPPC  P  V+GLMPHTDSDFLTIL+QDQVGGL+L+K
Sbjct: 181 SGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIK 240

Query: 241 DGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVI 300
           D  WIAVKPNP+ALIINIGDLFQAWSN +YKSVEHRV+TN K+ERFS AYF+CP  + VI
Sbjct: 241 DNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVI 300

Query: 301 KSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFV 329
           +   +   YR FSFREFR QVQEDV+K GFK+GLP+F+
Sbjct: 301 ECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.6e-92
Identity = 178/348 (51.15%), Postives = 229/348 (65.80%), Query Frame = 0


Query: 1   MDPPFHEAYKSL----------LAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVE 60
           +DPP  ++Y++L          +A  AV   G   AV E +LP+VDL RL  G   GE +
Sbjct: 9   IDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVLERDLPMVDLERLTRGG-AGERK 68

Query: 61  QCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSY 120
            C   + RA+ EWGFFQ+ NHGV  EL+ +MR +Q  LF+ PFE K K  + LN   GSY
Sbjct: 69  ACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAGL-LN---GSY 128

Query: 121 RWGTPTATSLTQLSWSEAFHVSLSDILGSN----GSDDLRSTMEEFAGKVSRLAQELAEI 180
           RWG PTATSL  LSWSEAFHV L+ I G++        LR  M+E A  +SR+A  +A  
Sbjct: 129 RWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANTVAAA 188

Query: 181 LGENL-GR------SSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILH 240
           L E L GR      ++ +F   C  +TC+LR+NRYP C      FGL+PHTDSDFLT+L 
Sbjct: 189 LAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLC 248

Query: 241 QDQVGGLELVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIA 300
           QDQVGGL L+KD  W+AV+P P+AL++NIGDLFQAWSN+ YKSVEH+VV N+K +R S+A
Sbjct: 249 QDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDRLSVA 308

Query: 301 YFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKF 328
           YFLCP  ++++ +  EP  YR F+F E+R +VQEDVR  G KIGLP F
Sbjct: 309 YFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNF 351

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.1e-84
Identity = 166/335 (49.55%), Postives = 215/335 (64.18%), Query Frame = 0


Query: 2   DPPFHEAYKSLLAET-AVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 61
           +PP    YK L  E   +D    A+ V E ELPV+DL  L +  +V     C+ D++RA+
Sbjct: 11  NPPLMSTYKHLFVEQHRLDMDMGAIDVDECELPVIDLAGLMEAEQV-----CRADMVRAA 70

Query: 62  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 121
            EWGFFQV NHGV   LL ++   Q+ +F++PF+ K  E + L FS  SYRWGTPTA  L
Sbjct: 71  SEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTERL-LGFSPESYRWGTPTAKCL 130

Query: 122 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENL-GRSSKFFV 181
            QLSWSEA+H+ ++    S  S   R+ +EE +  +  LAQ+LAEIL   L G      +
Sbjct: 131 EQLSWSEAYHIPMTTPRPST-SIRARAVIEEVSRAMYELAQKLAEILMRGLPGAGEGETM 190

Query: 182 DNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILH--QDQVGGLELVKDGEWIAV 241
                 TC+LR+NRYPPC++    FGL PHTDSD LTI+H  QD VGGL+L+K G W+AV
Sbjct: 191 VTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAV 250

Query: 242 KPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKC--- 301
           KP+P  LI+N+GDL QAWSNDVYKSVEHRV+ N+ LERFS+A+FLCP   T+I       
Sbjct: 251 KPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSHV 310

Query: 302 --EPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKF 328
             +   YR F+F E+R Q+ EDVR  G KIGL +F
Sbjct: 311 HDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRF 338

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.6e-77
Identity = 142/300 (47.33%), Postives = 208/300 (69.33%), Query Frame = 0


Query: 31  ELPVVDLRRLRDGAEVGEVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFK 90
           +LPV+DL  L  G EV + ++C   ++ A++EWGFFQ+VNHG+  ++   M  ++ +LF 
Sbjct: 39  KLPVIDLSHLTSGEEV-KRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 98

Query: 91  QPFERKSKEDMFLNFSAGSYRWGTPTATSLTQLSWSEAFHVSLSDILG-SNGSDDLRSTM 150
           QPF  K +E  F + S  SYRWG P+ATS  Q S SEAFH+ LS++   S+  ++LR+ +
Sbjct: 99  QPFSVKVRE-RFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIV 158

Query: 151 EEFAGKVSRLAQELAEILGENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPH 210
           E +  +++R+AQ + EILG+ +  SS++F +       +LR+N+Y P      VFGL+PH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 211 TDSDFLTILHQDQVGGLELVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVT 270
           TD+ FLTIL QDQ+GGLEL  +G+WI+VKP  EAL +NIGD+FQA SN VY+SV HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 271 NSKLERFSIAYFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 330
            + +ER SIA+F+CP+ ET I     P  YRRFSFRE++ Q + DV++ G K+GL +F++
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy Swiss-Prot
Match: Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 2.1e-39
Identity = 93/281 (33.10%), Postives = 140/281 (49.82%), Query Frame = 0


Query: 24  AVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRA 83
           ++ V E ++P++DL+ L   ++          I  A ++ GFF VVNHG+S EL+     
Sbjct: 53  SINVLELDVPLIDLQNLL--SDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHE 112

Query: 84  KQIELFKQPFERKSKEDMFLNFSAGSYRWGTP-TATSLTQLSWSEAFHVSLSDILG---- 143
                F  P   K +    L  S  S  + +  T    T+L W E       D +     
Sbjct: 113 YTSRFFDMPLSEKQR---VLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKS 172

Query: 144 ---------SNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVDNCVPSTCYL 203
                     +G        +E+   +S L+ ++ E+LG +LG    +F +    +   +
Sbjct: 173 VQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIM 232

Query: 204 RMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPNPEALIINIG 263
           R+N YPPC  P    G  PH D   LTILHQD V GL++  + +W +++PNP+A ++NIG
Sbjct: 233 RLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIG 292

Query: 264 DLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVI 291
           D F A SND YKS  HR V NS+ ER S+A+FLCP  + V+
Sbjct: 293 DTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVV 328

[Close]

BLAST of Cp4.1LG20g03710 vs. NCBI nr
Match: XP_023520137.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674 bits (1738), Expect = 2.48e-244
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60
           MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS
Sbjct: 13  MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 72

Query: 61  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120
           QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL
Sbjct: 73  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 132

Query: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180
           TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD
Sbjct: 133 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 192

Query: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240
           NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN
Sbjct: 193 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 252

Query: 241 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 300
           PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR
Sbjct: 253 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 312

Query: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RFSFREFRNQVQEDVRKHGFKIGLPKFVL
Sbjct: 313 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of Cp4.1LG20g03710 vs. NCBI nr
Match: KAG6584068.1 (Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019669.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 667 bits (1722), Expect = 4.34e-242
Identity = 325/329 (98.78%), Postives = 328/329 (99.70%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60
           MDPPFHEAYKSLLAETAV+RGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS
Sbjct: 1   MDPPFHEAYKSLLAETAVERGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60

Query: 61  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120
           QEWGFFQVVNHGVSS+LLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL
Sbjct: 61  QEWGFFQVVNHGVSSQLLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120

Query: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180
           TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD
Sbjct: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180

Query: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240
           NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN
Sbjct: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240

Query: 241 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 300
           PEALI+NIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCE GVYR
Sbjct: 241 PEALIVNIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCESGVYR 300

Query: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RFSFREFRNQVQEDVRKHGFKIGLPKFVL
Sbjct: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329

[Close]

BLAST of Cp4.1LG20g03710 vs. NCBI nr
Match: XP_023001285.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita maxima])

HSP 1 Score: 665 bits (1716), Expect = 5.59e-241
Identity = 323/329 (98.18%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60
           MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAE GEVEQCKNDIIRAS
Sbjct: 13  MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEAGEVEQCKNDIIRAS 72

Query: 61  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120
           QEWGFFQVVNHGVSS++LGKMRAKQIELFKQPFERKSKEDMFLNFSAGSY WGTPTATSL
Sbjct: 73  QEWGFFQVVNHGVSSQVLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYCWGTPTATSL 132

Query: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180
           TQLSWSEAFH+SLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD
Sbjct: 133 TQLSWSEAFHISLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 192

Query: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240
           NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN
Sbjct: 193 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 252

Query: 241 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 300
           PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVY+
Sbjct: 253 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYK 312

Query: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RFSFREFRNQVQEDVRKHGFKIGLPKFVL
Sbjct: 313 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of Cp4.1LG20g03710 vs. NCBI nr
Match: XP_022933193.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita moschata])

HSP 1 Score: 665 bits (1715), Expect = 7.94e-241
Identity = 324/329 (98.48%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60
           MDPPFHEAYKSLLAETAV+RGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS
Sbjct: 13  MDPPFHEAYKSLLAETAVERGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 72

Query: 61  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120
           QEWGFFQVVNHGVSS+LLGKMRAKQI LFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL
Sbjct: 73  QEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 132

Query: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180
           TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD
Sbjct: 133 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 192

Query: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240
           NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN
Sbjct: 193 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 252

Query: 241 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 300
           PEALIINIGDLFQAWSN+VYKSVEHRVVTNSKLERFSIAYF CPWSETVIKSKCEPGVYR
Sbjct: 253 PEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFCPWSETVIKSKCEPGVYR 312

Query: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RFSFREFRNQVQEDVRKHGFKIGLPKFVL
Sbjct: 313 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of Cp4.1LG20g03710 vs. NCBI nr
Match: XP_038894099.1 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Benincasa hispida])

HSP 1 Score: 599 bits (1544), Expect = 9.16e-215
Identity = 287/330 (86.97%), Postives = 314/330 (95.15%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETA-VDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRA 60
           MDPPFHEAYK+LL ETA V RG + V V+EWELPVVDLRRL+  A V EVE+CK DII+A
Sbjct: 13  MDPPFHEAYKALLGETAAVPRGSEVVTVEEWELPVVDLRRLKAVAAV-EVEECKKDIIKA 72

Query: 61  SQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATS 120
           S+EWGFFQV+NHG+S++LL KMRAKQ+ELFKQPFERKSKED FLNFSAGSYRWGTP+ATS
Sbjct: 73  SKEWGFFQVLNHGISNQLLAKMRAKQMELFKQPFERKSKEDQFLNFSAGSYRWGTPSATS 132

Query: 121 LTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFV 180
           +TQLSWSEAFHVSLSDILGSNGSD+LRSTMEEFAGKVSRLAQ+LA ILGENLGRS+KFFV
Sbjct: 133 ITQLSWSEAFHVSLSDILGSNGSDELRSTMEEFAGKVSRLAQKLAAILGENLGRSAKFFV 192

Query: 181 DNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKP 240
           +NCVPSTCYLRMNRYPPC VPG++FGLMPHTDSDFLTILHQDQVGGLELVKDG+W+AVKP
Sbjct: 193 ENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWVAVKP 252

Query: 241 NPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVY 300
           NP+ALI+NIGDLFQAWSNDVYKSVEHRVVTNSK+ER+SIAYFLCPWSETVIKSKCEPGVY
Sbjct: 253 NPDALIVNIGDLFQAWSNDVYKSVEHRVVTNSKVERYSIAYFLCPWSETVIKSKCEPGVY 312

Query: 301 RRFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RRFSFREFRNQVQEDVRKHG KIGLP+FVL
Sbjct: 313 RRFSFREFRNQVQEDVRKHGHKIGLPRFVL 341

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy TrEMBL
Match: A0A6J1KKS1 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita maxima OX=3661 GN=LOC111495461 PE=3 SV=1)

HSP 1 Score: 665 bits (1716), Expect = 2.71e-241
Identity = 323/329 (98.18%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60
           MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAE GEVEQCKNDIIRAS
Sbjct: 13  MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEAGEVEQCKNDIIRAS 72

Query: 61  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120
           QEWGFFQVVNHGVSS++LGKMRAKQIELFKQPFERKSKEDMFLNFSAGSY WGTPTATSL
Sbjct: 73  QEWGFFQVVNHGVSSQVLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYCWGTPTATSL 132

Query: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180
           TQLSWSEAFH+SLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD
Sbjct: 133 TQLSWSEAFHISLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 192

Query: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240
           NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN
Sbjct: 193 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 252

Query: 241 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 300
           PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVY+
Sbjct: 253 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYK 312

Query: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RFSFREFRNQVQEDVRKHGFKIGLPKFVL
Sbjct: 313 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy TrEMBL
Match: A0A6J1F422 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 PE=3 SV=1)

HSP 1 Score: 665 bits (1715), Expect = 3.85e-241
Identity = 324/329 (98.48%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 60
           MDPPFHEAYKSLLAETAV+RGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS
Sbjct: 13  MDPPFHEAYKSLLAETAVERGGDAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRAS 72

Query: 61  QEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 120
           QEWGFFQVVNHGVSS+LLGKMRAKQI LFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL
Sbjct: 73  QEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNFSAGSYRWGTPTATSL 132

Query: 121 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 180
           TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD
Sbjct: 133 TQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVD 192

Query: 181 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 240
           NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN
Sbjct: 193 NCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPN 252

Query: 241 PEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEPGVYR 300
           PEALIINIGDLFQAWSN+VYKSVEHRVVTNSKLERFSIAYF CPWSETVIKSKCEPGVYR
Sbjct: 253 PEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFCPWSETVIKSKCEPGVYR 312

Query: 301 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           RFSFREFRNQVQEDVRKHGFKIGLPKFVL
Sbjct: 313 RFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy TrEMBL
Match: A0A5A7UM12 (Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006030 PE=3 SV=1)

HSP 1 Score: 578 bits (1491), Expect = 3.78e-207
Identity = 282/333 (84.68%), Postives = 307/333 (92.19%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGG--DAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIR 60
           MDPPFHEAYK+LLAETA +  G  D V V+EW+LPVVDL RL  G +V E E+CKNDIIR
Sbjct: 1   MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLT-GGKVEEEEECKNDIIR 60

Query: 61  ASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTAT 120
           AS+EWGFFQVVNHG+S++LL KMRAKQIELFKQPFERKSKED F NFSAGSYRWGTP+AT
Sbjct: 61  ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSAT 120

Query: 121 SLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSK 180
           S+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQ+LAEILGENLGRSSK
Sbjct: 121 SITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSK 180

Query: 181 FFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIA 240
           FFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLELVKDG+WIA
Sbjct: 181 FFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIA 240

Query: 241 VKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEP 300
           VKPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCPWSETVIKSK EP
Sbjct: 241 VKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEP 300

Query: 301 GVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           GVYRRFSFREFRNQVQEDVRK+G+KIGLP+FVL
Sbjct: 301 GVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy TrEMBL
Match: A0A1S3B309 (gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 578 bits (1491), Expect = 6.11e-207
Identity = 282/333 (84.68%), Postives = 307/333 (92.19%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGG--DAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIR 60
           MDPPFHEAYK+LLAETA +  G  D V V+EW+LPVVDL RL  G +V E E+CKNDIIR
Sbjct: 14  MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLT-GGKVEEEEECKNDIIR 73

Query: 61  ASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTAT 120
           AS+EWGFFQVVNHG+S++LL KMRAKQIELFKQPFERKSKED F NFSAGSYRWGTP+AT
Sbjct: 74  ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSAT 133

Query: 121 SLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSK 180
           S+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQ+LAEILGENLGRSSK
Sbjct: 134 SITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSK 193

Query: 181 FFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIA 240
           FFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLELVKDG+WIA
Sbjct: 194 FFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIA 253

Query: 241 VKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEP 300
           VKPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCPWSETVIKSK EP
Sbjct: 254 VKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEP 313

Query: 301 GVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           GVYRRFSFREFRNQVQEDVRK+G+KIGLP+FVL
Sbjct: 314 GVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

[Close]

BLAST of Cp4.1LG20g03710 vs. ExPASy TrEMBL
Match: A0A1S3B3F3 (gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 578 bits (1491), Expect = 1.98e-206
Identity = 282/333 (84.68%), Postives = 307/333 (92.19%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGG--DAVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIR 60
           MDPPFHEAYK+LLAETA +  G  D V V+EW+LPVVDL RL  G +V E E+CKNDIIR
Sbjct: 46  MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLT-GGKVEEEEECKNDIIR 105

Query: 61  ASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPTAT 120
           AS+EWGFFQVVNHG+S++LL KMRAKQIELFKQPFERKSKED F NFSAGSYRWGTP+AT
Sbjct: 106 ASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSAT 165

Query: 121 SLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSK 180
           S+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQ+LAEILGENLGRSSK
Sbjct: 166 SITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSK 225

Query: 181 FFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIA 240
           FFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLELVKDG+WIA
Sbjct: 226 FFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIA 285

Query: 241 VKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIKSKCEP 300
           VKPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCPWSETVIKSK EP
Sbjct: 286 VKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEP 345

Query: 301 GVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 329
           GVYRRFSFREFRNQVQEDVRK+G+KIGLP+FVL
Sbjct: 346 GVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

[Close]

BLAST of Cp4.1LG20g03710 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 429.9 bits (1104), Expect = 1.9e-120
Identity = 207/338 (61.24%), Postives = 254/338 (75.15%), Query Frame = 0


Query: 1   MDPPFHEAYKSLLAETAVDRGGD----------AVAVKEWELPVVDLRRLRDGAEVGEVE 60
           MDPPF+E Y +LL      +  D             V+E ELPV+D+ RL DGAE  E E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAE-EERE 60

Query: 61  QCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSY 120
           +CK  I RAS+EWGFFQV+NHG+S ++L KMR +QI +F++PF++KSK +    FSAGSY
Sbjct: 61  KCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSE---KFSAGSY 120

Query: 121 RWGTPTATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEILGEN 180
           RWGTP+ATS+ QLSWSEAFHV ++DI  +     L STME+FA +   LA  LAE+L E 
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEK 180

Query: 181 LGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVK 240
            G++S FF +NCV +TCYLRMNRYPPC  P  V+GLMPHTDSDFLTIL+QDQVGGL+L+K
Sbjct: 181 SGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIK 240

Query: 241 DGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVI 300
           D  WIAVKPNP+ALIINIGDLFQAWSN +YKSVEHRV+TN K+ERFS AYF+CP  + VI
Sbjct: 241 DNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVI 300

Query: 301 KSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFV 329
           +   +   YR FSFREFR QVQEDV+K GFK+GLP+F+
Sbjct: 301 ECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFL 334

[Close]

BLAST of Cp4.1LG20g03710 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 289.3 bits (739), Expect = 4.0e-78
Identity = 142/300 (47.33%), Postives = 208/300 (69.33%), Query Frame = 0


Query: 31  ELPVVDLRRLRDGAEVGEVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFK 90
           +LPV+DL  L  G EV + ++C   ++ A++EWGFFQ+VNHG+  ++   M  ++ +LF 
Sbjct: 39  KLPVIDLSHLTSGEEV-KRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 98

Query: 91  QPFERKSKEDMFLNFSAGSYRWGTPTATSLTQLSWSEAFHVSLSDILG-SNGSDDLRSTM 150
           QPF  K +E  F + S  SYRWG P+ATS  Q S SEAFH+ LS++   S+  ++LR+ +
Sbjct: 99  QPFSVKVRE-RFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIV 158

Query: 151 EEFAGKVSRLAQELAEILGENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPH 210
           E +  +++R+AQ + EILG+ +  SS++F +       +LR+N+Y P      VFGL+PH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 211 TDSDFLTILHQDQVGGLELVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVT 270
           TD+ FLTIL QDQ+GGLEL  +G+WI+VKP  EAL +NIGD+FQA SN VY+SV HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 271 NSKLERFSIAYFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 330
            + +ER SIA+F+CP+ ET I     P  YRRFSFRE++ Q + DV++ G K+GL +F++
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of Cp4.1LG20g03710 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 6.4e-44
Identity = 125/356 (35.11%), Postives = 171/356 (48.03%), Query Frame = 0


Query: 7   EAYKSLLAETAVDRGGDAVAV----KEWELPVVDLRRLRDGAEVGEVEQCKNDIIRASQE 66
           E+Y         D    A  V    K+  +PV+DL RL            K  +  A +E
Sbjct: 5   ESYPPAFRRVTNDENPPATPVIVQGKDINIPVIDLERLD-----------KEILREACKE 64

Query: 67  WGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSAGSYRWGTPT------ 126
           WG F++ NHGV   L  +++     L   PFE+K +E      S  SY WGTP       
Sbjct: 65  WGIFRLENHGVPLALTSRLQEISESLLSLPFEKK-RELFAAVKSPLSYFWGTPALNRSGD 124

Query: 127 ----ATSLTQLSWSEAFHV------SLSDILGSNGSDD----------LRSTMEEFAGKV 186
                   + L+  E F+V      SLS +  S   DD           R  MEE+   +
Sbjct: 125 ALKRGAQASNLTMLEGFNVPLSSLSSLSKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHI 184

Query: 187 SRLAQELAEILGE--NLGRSSKFFVDNCVPSTCYLRMNRYPPCS--VPGRVFGLMPHTDS 246
           +R+A  L E + +  NL  S     +    ST  +R+ RYP  S        G+  HTDS
Sbjct: 185 TRIAVSLFEAIAQTLNLELSGNRRSEYLSESTGLIRVYRYPQSSEEAAREALGMEVHTDS 244

Query: 247 DFLTILHQDQVGGLELVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRV-VTNS 306
             ++IL +D+ GGLE++K  EW  VKP    LI+N+GD+ QA S+D YKSV HRV   N 
Sbjct: 245 SVISILREDESGGLEIMKGEEWFCVKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNR 304

Query: 307 KLERFSIAYFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKF 328
           K ER S+ YF+ P  + VIKS      Y+ F++ +F  QVQ DV+  G KIGLP+F
Sbjct: 305 KTERHSVCYFVFPKRDCVIKS----SNYKLFTYSDFEAQVQADVQSLGTKIGLPRF 344

[Close]

BLAST of Cp4.1LG20g03710 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-40
Identity = 93/281 (33.10%), Postives = 140/281 (49.82%), Query Frame = 0


Query: 24  AVAVKEWELPVVDLRRLRDGAEVGEVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRA 83
           ++ V E ++P++DL+ L   ++          I  A ++ GFF VVNHG+S EL+     
Sbjct: 53  SINVLELDVPLIDLQNLL--SDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHE 112

Query: 84  KQIELFKQPFERKSKEDMFLNFSAGSYRWGTP-TATSLTQLSWSEAFHVSLSDILG---- 143
                F  P   K +    L  S  S  + +  T    T+L W E       D +     
Sbjct: 113 YTSRFFDMPLSEKQR---VLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKS 172

Query: 144 ---------SNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVDNCVPSTCYL 203
                     +G        +E+   +S L+ ++ E+LG +LG    +F +    +   +
Sbjct: 173 VQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREFFEENDSIM 232

Query: 204 RMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVKDGEWIAVKPNPEALIINIG 263
           R+N YPPC  P    G  PH D   LTILHQD V GL++  + +W +++PNP+A ++NIG
Sbjct: 233 RLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNPKAFVVNIG 292

Query: 264 DLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVI 291
           D F A SND YKS  HR V NS+ ER S+A+FLCP  + V+
Sbjct: 293 DTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVV 328

[Close]

BLAST of Cp4.1LG20g03710 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 4.3e-40
Identity = 103/315 (32.70%), Postives = 153/315 (48.57%), Query Frame = 0


Query: 31  ELPVVDLRRLRDGAEVGEVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFK 90
           E+PV+DL RL D  +V  V    ++I  A ++WGFFQV+NHGV S+   ++       F 
Sbjct: 27  EIPVIDLSRLDDPEDVQNV---ISEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFD 86

Query: 91  QPFERKSKEDMFLNFSAGSYRWGTPTATSLTQLSWSEAFHVSLSDIL------------- 150
            P E K K     + +   Y  G  T        W E F +   D +             
Sbjct: 87  LPMEEKIKVKRD-DVNPVGYHDGEHTK---NVKDWKEVFDIYFKDPMVIPSTTDPEDEGL 146

Query: 151 ------GSNGSDDLRSTMEEFAGKVSRLAQELAEILGENLGRSSKFFVDNCVPSTCYLRM 210
                       D R   E +A    +LA +L E++  +LG   + F D       + R+
Sbjct: 147 RLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRI 206

Query: 211 NRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLELVK--DGEWIAVKPNPEALIINIG 270
           NRYPPC  P    G+  H D+D +++L QD VGGL++ +  DG W  ++P P AL+INIG
Sbjct: 207 NRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIG 266

Query: 271 DLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSETVIK------SKCEPGVYRRFS 318
           +  + W+ND Y S EHRVV N+  ER+SI +FL P  +  +K      S   P  Y+ + 
Sbjct: 267 NCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSPENPPKYKGYK 326

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g03710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	2.6e-119	61.24	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q7XP65	1.6e-92	51.15	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	8.1e-84	49.55	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9C6I4	5.6e-77	47.33	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q39110	2.1e-39	33.10	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]

BLAST of Cp4.1LG20g03710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520137.1	2.48e-244	100.00	gibberellin 2-beta-dioxygenase 8 [Cucurbita pepo subsp. pepo]	[more]
KAG6584068.1	4.34e-242	98.78	Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_023001285.1	5.59e-241	98.18	gibberellin 2-beta-dioxygenase 8 [Cucurbita maxima]	[more]
XP_022933193.1	7.94e-241	98.48	gibberellin 2-beta-dioxygenase 8 [Cucurbita moschata]	[more]
XP_038894099.1	9.16e-215	86.97	gibberellin 2-beta-dioxygenase 8 isoform X1 [Benincasa hispida]	[more]

BLAST of Cp4.1LG20g03710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KKS1	2.71e-241	98.18	gibberellin 2-beta-dioxygenase 8 OS=Cucurbita maxima OX=3661 GN=LOC111495461 PE=...	[more]
A0A6J1F422	3.85e-241	98.48	gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 P...	[more]
A0A5A7UM12	3.78e-207	84.68	Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B309	6.11e-207	84.68	gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A1S3B3F3	1.98e-206	84.68	gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]

BLAST of Cp4.1LG20g03710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	1.9e-120	61.24	gibberellin 2-oxidase 8	[more]
AT1G50960.1	4.0e-78	47.33	gibberellin 2-oxidase 7	[more]
AT5G58660.1	6.4e-44	35.11	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G25420.1	1.5e-40	33.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	4.3e-40	32.70	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 190..284 e-value: 1.6E-27 score: 96.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 30..322 e-value: 1.2E-77 score: 263.3
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 32..133 e-value: 4.5E-15 score: 56.4
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 18..322
None	No IPR available	PANTHER	PTHR47990:SF13	GIBBERELLIN 2-BETA-DIOXYGENASE 8-LIKE	coord: 18..322
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 27..313
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 185..284 score: 15.101486

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g03710.1	Cp4.1LG20g03710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding