Cp4.1LG20g03470 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g03470
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionHigh-affinity nitrate transporter
LocationCp4.1LG20: 1957893 .. 1959234 (-)
RNA-Seq ExpressionCp4.1LG20g03470
SyntenyCp4.1LG20g03470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGTCATCGTCGTCGTCGTCCTCAAGTCTGCTGAAATATGGAGGGTTCAATCAATATGTCTGCTAATGGGTTTCTCTTGGCTGCTCTGTTTCTGTTCTGTTTCTTCGGATTCTCTCATGGACATATCCACTTTTCGTCTCTTCCAAGAAGTTTGGAGGTCACTGCTTCGCCTAAACCTGGACAAGGTAAGATTTTTCAGCCCCACCTTTAGATATTTGTAACACCTTTAGATATCTTGTAACATTTTCCTAATAGACACGTTGTGTGGAAGCCAAGAGGGAAAGCCAAAAAGAAGAATATCTACGTTGATGAGTTTGGGCAGTTACAAATAGTAATAGAGTCAGTGATAAGGCTGAGTTCCGAAGGGGGATGAATAGGGGGCGGTGTGACAACAAGAACGCTGCCCGAAGGGGGATGGATAGGGAGCGGTGTGACAACAAGAACGCTGGACCCAAAGGGATGGATTTGGGGTGGACGGTGAAATCACACCTCGAAGAGAGAAAAGTTTTGAAAACTCGAAAGGGAAAGCATATCGATCTGAATCCAAAATTGGAACTTTACCCATTTCTAACAACTGGGTTGTCTTTTTTTTCTGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGATTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAGTGGACCGGGCATGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGCGAACAAGACGGTACAAAAGTTTGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACAAACTTGTTTCAAATCGAATCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTGGCCGGAAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGGACAGTAAGTTAACAAAGATGGAAACTTGCTCTGTTTCTGAGTTCTTTTTTTTAAGTCTTTCTGCATTTTGGGTTTCTCTTGTACTGTAATTTTCTAGGTGAAAAGATGGAAAATAAAGTGTATTAAATTATGAATGTGTTGATTAATGACAATATTTGAATATATTGGACTTTGGGGTTTCATTCATTAGGGTAGATACATAGCTTGAATGGAACACAAAAGGTTGCAACACTCCAACCTTAGAACA

mRNA sequence

ATGAGTGTCATCGTCGTCGTCGTCCTCAAGTCTGCTGAAATATGGAGGGTTCAATCAATATTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGATTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAGTGGACCGGGCATGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGCGAACAAGACGGTACAAAAGTTTGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACAAACTTGTTTCAAATCGAATCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTGGCCGGAAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGGACAGTAAGTTAACAAAGATGGAAACTTGCTCTGTTTCTGAGTTCTTTTTTTTAAGTCTTTCTGCATTTTGGGTTTCTCTTGTACTGTAATTTTCTAGGTGAAAAGATGGAAAATAAAGTGTATTAAATTATGAATGTGTTGATTAATGACAATATTTGAATATATTGGACTTTGGGGTTTCATTCATTAGGGTAGATACATAGCTTGAATGGAACACAAAAGGTTGCAACACTCCAACCTTAGAACA

Coding sequence (CDS)

ATGAGTGTCATCGTCGTCGTCGTCCTCAAGTCTGCTGAAATATGGAGGGTTCAATCAATATTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGATTCTAAACGGGACTGTCAAATCTGGGTCGGATTCGAGCTACAAGACCGTCGAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAAGTGGACCGGGCATGGAGAAAAACAGAGGACGATCTCTCAAAGGACAAGACCTGCCAGTTCCTCATTGTGGGAAAGCCGTACAATCCGGCGAACAAGACGGTACAAAAGTTTGAGTGGACGGTGGCGCGTGACATTCCAACGGGAACTTTCTTCGTCCGGGCCTACGCTTTCAATTCCGCCGGCGAGGAAGTGGGCTACGGGCAAACGACTGACAACAAAAAAGGCACAAACTTGTTTCAAATCGAATCGATCACAGGTCGCCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTGCTGTCTTTGTCCGGATTCTTCTTGGCCGGAAAGAGAAAGGCCAAGAAGGCGGAGGCGCCGGGACAGTAA

Protein sequence

MSVIVVVVLKSAEIWRVQSIFLKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ
Homology
[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 2.9e-46
Identity = 90/160 (56.25%), Postives = 120/160 (75.00%), Query Frame = 0 

Query: 20  IFLKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTC 79
           + L AG D +++TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC
Sbjct: 50  VVLDAGKDTLNITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTC 109

Query: 80  QFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNL 139
              I+ KPY   +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNL
Sbjct: 110 PHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNL 169

Query: 140 FQIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
           F +++I+GRH SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 170 FSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.5e-42
Identity = 84/159 (52.83%), Postives = 114/159 (71.70%), Query Frame = 0 

Query: 22  LKAGVDKISVTWIL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQ 81
           L+AG D +++TW L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C 
Sbjct: 49  LEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCP 108

Query: 82  FLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLF 141
             IV K Y   +KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ+TD  + TNLF
Sbjct: 109 HEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLF 168

Query: 142 QIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
            + +I+G H+ LDIAS  FS FSV+SL  FF+  KRKAK
Sbjct: 169 SVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.1e-35
Identity = 73/157 (46.50%), Postives = 105/157 (66.88%), Query Frame = 0 

Query: 24  AGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLI 83
           AG + ++VTW LN +  +G+D+++K+V+ KLCYAP S+ DR WRK  DDL KDK CQF +
Sbjct: 57  AGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPASRTDRGWRKASDDLHKDKACQFKV 116

Query: 84  VGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIE 143
             +PY        +F++ VARDIPT ++FVRAYA +++G EV YGQ++ +      F + 
Sbjct: 117 TVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDASGTEVAYGQSSPDA----AFDVA 176

Query: 144 SITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKK 181
            ITG H SL +A+  FS FS+ +L+ FF+  KRK  K
Sbjct: 177 GITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 4.3e-34
Identity = 77/162 (47.53%), Postives = 103/162 (63.58%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGT---VKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKT 81
           L AG D I+VTW LN T     +G+D+ YK V+  LCYAP SQV R WRK  DDLSKDK 
Sbjct: 57  LHAGEDAITVTWSLNATAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKA 116

Query: 82  CQFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTN 141
           CQF I  +PY+ A     KFE+TVARD+PT +++VRAYA +++G  V YG+T  +     
Sbjct: 117 CQFKIAQQPYDGAG----KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS--- 176

Query: 142 LFQIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKK 181
            F +  ITG   S+++A+   SAFSV +L+ F +   +K  K
Sbjct: 177 -FAVAGITGVTASIEVAAGVLSAFSVAALAVFLVLENKKKNK 210

[Close]
BLAST of Cp4.1LG20g03470 vs. NCBI nr
Match: XP_023520111.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333 bits (853), Expect = 5.91e-114
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF
Sbjct: 51  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 110

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
           LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ
Sbjct: 111 LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 170

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ
Sbjct: 171 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]
BLAST of Cp4.1LG20g03470 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 328 bits (842), Expect = 2.80e-112
Identity = 161/165 (97.58%), Postives = 164/165 (99.39%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTW+LNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF
Sbjct: 51  LKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 110

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
           LIVGKPYNP NKTVQKFEWTVARDIPTGTFFVRAYAFNSAG+EVGYGQTTDNKKGTNLFQ
Sbjct: 111 LIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQ 170

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IE+ITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ
Sbjct: 171 IEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]
BLAST of Cp4.1LG20g03470 vs. NCBI nr
Match: XP_023001304.1 (high-affinity nitrate transporter 3.1-like [Cucurbita maxima])

HSP 1 Score: 327 bits (837), Expect = 1.62e-111
Identity = 160/165 (96.97%), Postives = 164/165 (99.39%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTW+LNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF
Sbjct: 51  LKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 110

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
           LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQT+DNKKGTNLFQ
Sbjct: 111 LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQ 170

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           I++ITGRHISLDIASVCFSAFSVLSLSGFFLAGKRK KKAEAPGQ
Sbjct: 171 IKAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKTKKAEAPGQ 215

[Close]
BLAST of Cp4.1LG20g03470 vs. NCBI nr
Match: KAG6584111.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019702.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327 bits (837), Expect = 1.62e-111
Identity = 161/165 (97.58%), Postives = 163/165 (98.79%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTW+LNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF
Sbjct: 51  LKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 110

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
           LIVGKPYNP NKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ
Sbjct: 111 LIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 170

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IE+ITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAP Q
Sbjct: 171 IEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPEQ 215

[Close]
BLAST of Cp4.1LG20g03470 vs. NCBI nr
Match: XP_008439011.1 (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa] >TYJ99190.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 286 bits (732), Expect = 1.48e-95
Identity = 138/165 (83.64%), Postives = 148/165 (89.70%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKI VTW+LNGTVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF
Sbjct: 45  LKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQF 104

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
            IV KPYNPANKT Q FEWTV RD+PTGTFFVRAY FNSAG+EV YGQTTD+KKGTNLFQ
Sbjct: 105 DIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQ 164

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IESITGRH+SLDIASVCFSAFSV+SL GFF   KRKAKKA A  +
Sbjct: 165 IESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 328 bits (842), Expect = 1.36e-112
Identity = 161/165 (97.58%), Postives = 164/165 (99.39%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTW+LNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF
Sbjct: 51  LKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 110

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
           LIVGKPYNP NKTVQKFEWTVARDIPTGTFFVRAYAFNSAG+EVGYGQTTDNKKGTNLFQ
Sbjct: 111 LIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQ 170

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IE+ITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ
Sbjct: 171 IEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy TrEMBL
Match: A0A6J1KMC9 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE=3 SV=1)

HSP 1 Score: 327 bits (837), Expect = 7.84e-112
Identity = 160/165 (96.97%), Postives = 164/165 (99.39%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTW+LNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF
Sbjct: 51  LKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 110

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
           LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQT+DNKKGTNLFQ
Sbjct: 111 LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQ 170

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           I++ITGRHISLDIASVCFSAFSVLSLSGFFLAGKRK KKAEAPGQ
Sbjct: 171 IKAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKTKKAEAPGQ 215

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 286 bits (732), Expect = 7.17e-96
Identity = 138/165 (83.64%), Postives = 148/165 (89.70%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKI VTW+LNGTVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF
Sbjct: 45  LKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQF 104

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
            IV KPYNPANKT Q FEWTV RD+PTGTFFVRAY FNSAG+EV YGQTTD+KKGTNLFQ
Sbjct: 105 DIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQ 164

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IESITGRH+SLDIASVCFSAFSV+SL GFF   KRKAKKA A  +
Sbjct: 165 IESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 286 bits (732), Expect = 7.17e-96
Identity = 138/165 (83.64%), Postives = 148/165 (89.70%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKI VTW+LNGTVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF
Sbjct: 45  LKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQF 104

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
            IV KPYNPANKT Q FEWTV RD+PTGTFFVRAY FNSAG+EV YGQTTD+KKGTNLFQ
Sbjct: 105 DIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQ 164

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 186
           IESITGRH+SLDIASVCFSAFSV+SL GFF   KRKAKKA A  +
Sbjct: 165 IESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]
BLAST of Cp4.1LG20g03470 vs. ExPASy TrEMBL
Match: C8CHG8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2)

HSP 1 Score: 283 bits (723), Expect = 1.57e-94
Identity = 142/167 (85.03%), Postives = 147/167 (88.02%), Query Frame = 0 

Query: 22  LKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQF 81
           LKAGVDKISVTW+LN TVK+GSDSSYK ++AKLCYAPVSQVDRAWRKTEDDL KDKTCQF
Sbjct: 45  LKAGVDKISVTWVLNETVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQF 104

Query: 82  LIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQ 141
            IV K YNPANKTVQ FEWTV RDIPTGTFFVRAY  NSAGEEV YGQTTD KKGTNLFQ
Sbjct: 105 SIVEKQYNPANKTVQSFEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQ 164

Query: 142 IESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEA--PGQ 186
           IESITGRHISLDIAS CFSAFSV+SL GFF   KRKAKKA A  PGQ
Sbjct: 165 IESITGRHISLDIASACFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

[Close]
BLAST of Cp4.1LG20g03470 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 186.4 bits (472), Expect = 2.0e-47
Identity = 90/160 (56.25%), Postives = 120/160 (75.00%), Query Frame = 0 

Query: 20  IFLKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTC 79
           + L AG D +++TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC
Sbjct: 50  VVLDAGKDTLNITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTC 109

Query: 80  QFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNL 139
              I+ KPY   +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNL
Sbjct: 110 PHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNL 169

Query: 140 FQIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
           F +++I+GRH SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 170 FSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]
BLAST of Cp4.1LG20g03470 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 186.4 bits (472), Expect = 2.0e-47
Identity = 90/160 (56.25%), Postives = 120/160 (75.00%), Query Frame = 0 

Query: 20  IFLKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTC 79
           + L AG D +++TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC
Sbjct: 50  VVLDAGKDTLNITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTC 109

Query: 80  QFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNL 139
              I+ KPY   +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNL
Sbjct: 110 PHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNL 169

Query: 140 FQIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
           F +++I+GRH SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 170 FSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]
BLAST of Cp4.1LG20g03470 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 186.4 bits (472), Expect = 2.0e-47
Identity = 90/160 (56.25%), Postives = 120/160 (75.00%), Query Frame = 0 

Query: 20  IFLKAGVDKISVTWILNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTC 79
           + L AG D +++TW L+ ++ S  ++ +K ++ KLCYAP SQVDR WRKT D+L KDKTC
Sbjct: 50  VVLDAGKDTLNITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTC 109

Query: 80  QFLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNL 139
              I+ KPY   +KT+Q   WT+ RDIPTGT+FVRAYA ++ G EV YGQ+TD+ K TNL
Sbjct: 110 PHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNL 169

Query: 140 FQIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
           F +++I+GRH SLDIAS+CFS FSV++L  FF+  KRKAK
Sbjct: 170 FSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]
BLAST of Cp4.1LG20g03470 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-43
Identity = 84/159 (52.83%), Postives = 114/159 (71.70%), Query Frame = 0 

Query: 22  LKAGVDKISVTWIL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQ 81
           L+AG D +++TW L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C 
Sbjct: 283 LEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCP 342

Query: 82  FLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLF 141
             IV K Y   +KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ+TD  + TNLF
Sbjct: 343 HEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLF 402

Query: 142 QIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
            + +I+G H+ LDIAS  FS FSV+SL  FF+  KRKAK
Sbjct: 403 SVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 438

[Close]
BLAST of Cp4.1LG20g03470 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-43
Identity = 84/159 (52.83%), Postives = 114/159 (71.70%), Query Frame = 0 

Query: 22  LKAGVDKISVTWIL-NGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQ 81
           L+AG D +++TW L + + K  +D+++KT++ KLCYAP+SQVDR WRKT++ L KD++C 
Sbjct: 71  LEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCP 130

Query: 82  FLIVGKPYNPANKTVQKFEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLF 141
             IV K Y   +KT Q  +WT+  DIPTGT+FVRAY  +  G EV YGQ+TD  + TNLF
Sbjct: 131 HEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLF 190

Query: 142 QIESITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAK 180
            + +I+G H+ LDIAS  FS FSV+SL  FF+  KRKAK
Sbjct: 191 SVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 226

The following BLAST results are available for this feature:
BLAST of Cp4.1LG20g03470 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FGS52.9e-4656.25High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ... [more]
Q9SB671.5e-4252.83High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ... [more]
Q6ZI505.1e-3546.50High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ... [more]
Q7XK124.3e-3447.53Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ... [more]
BLAST of Cp4.1LG20g03470 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023520111.15.91e-114100.00high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo][more]
XP_022924070.12.80e-11297.58high-affinity nitrate transporter 3.1 [Cucurbita moschata][more]
XP_023001304.11.62e-11196.97high-affinity nitrate transporter 3.1-like [Cucurbita maxima][more]
KAG6584111.11.62e-11197.58High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_008439011.11.48e-9583.64PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266... [more]
BLAST of Cp4.1LG20g03470 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EDS11.36e-11297.58High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ... [more]
A0A6J1KMC97.84e-11296.97High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE... [more]
A0A5A7ULV77.17e-9683.64High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3AXC17.17e-9683.64High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S... [more]
C8CHG81.57e-9485.03High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2[more]
BLAST of Cp4.1LG20g03470 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G50200.32.0e-4756.25nitrate transmembrane transporters [more]
AT5G50200.22.0e-4756.25nitrate transmembrane transporters [more]
AT5G50200.12.0e-4756.25nitrate transmembrane transporters [more]
AT4G24730.11.1e-4352.83Calcineurin-like metallo-phosphoesterase superfamily protein [more]
AT4G24730.41.1e-4352.83Calcineurin-like metallo-phosphoesterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016605High-affinity nitrate transporterPFAMPF16974NAR2coord: 19..178
e-value: 6.0E-67
score: 224.4
IPR016605High-affinity nitrate transporterPIRSFPIRSF012939NAR2coord: 3..182
e-value: 9.0E-74
score: 245.0
IPR016605High-affinity nitrate transporterPANTHERPTHR34806HIGH-AFFINITY NITRATE TRANSPORTER 3.2coord: 18..181
NoneNo IPR availablePANTHERPTHR34806:SF1HIGH-AFFINITY NITRATE TRANSPORTER 3.2coord: 18..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g03470.1Cp4.1LG20g03470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity