Cp4.1LG20g03420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g03420
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Protein kinase domain-containing protein
Location	Cp4.1LG20: 1931784 .. 1935501 (+)
RNA-Seq Expression	Cp4.1LG20g03420
Synteny	Cp4.1LG20g03420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCCGCAACGTGGACGGTTGGATTTCCTCTCATCCTCCATATGCTTCGTTGTTAGGTTAAAATTAGGGTTGCGTTTCGATTCCATTCCACAAATTTCTTTCTGCGGAAGAATATTTCTTCTCATCTCCCTCTTCAAAGCCCCCGGAAATCTGGTTCTTCAATTCTCTTCTTCTTTTCAAGGCTCTGCTTCGTTGTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTCGATCGCAGGCCCCGAAAGAGGGCTCGCTTGGGCTGGGACGTTGTCCCCGAGGCCGCTAAGGTATAATCTCCGATCCTCTCTTTCAGATCTGATTTTTATGTTGATTTTGGTTTTGTAGAGCTACATCTGCTGGGTTTTTCTTTGATTAGGTATGTTTTAGTGTCGCGCACCGAGGTATAATCTTTTTGTTATTAGATTTACCTTGGTTGATTTACGTTTTTTTTTAAATTTTTTTTTCATTCAGTATTCTTGATAGGTTCTATGAGTCGAGGTTATTTTCTTGAAGTTTATTTACTGATCTGTTGTGTTAACGTATTTTTATTTTATTTGTAAGCTCAAAAGAAGATCAGGTAAGTATTGTTCTTTATTCAGATGGATGGTTAATTCCGAATTGCCTCTTCTTATTGATTATCTGTTGTTGATCCATTCCACGATTTGTTTTACTTAGGTGTCTCCCTTTATTTTCTACCTCATCCGTGTCTGTCTACCATGCATCTGGCTTATATGTATTTCTTTTTTCATTGTTCCAACAAAATCTTATAATTTCTTTTGTGTTGTACAAAATGAAGACTTTGGGTGAGGATATTAGGTTTAGGAAGGGCAAACTTTTACATGAATGGGTGACTGTGTGTAGGCTCAGGTAGGAATTTGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCACGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGACATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTGTAATTACACTTGACTATGATTAAACAAATTTTTATGCGCTATCAAAATTGATCACTGTAGAAATTTTCTCTTATAATTTAGTTCCTCATCTCATTTTTAATGTTGGATATTTCCTCAATTTTTTCTTACTCCCATAAATTGTATTTAAAATAATATCCATCATGATCACCCATGTTTATTTATTTTTGAACTGAACATTCAGACATAAGTTATGTTTTTATTTTCATAATCATTACTTGTTTAGGATGGTTAAAGGTTGGTTAGATTTTGGGAAATATCTATTTTCTTACTCTGTCAATTCTTTTTCTTCTGTGGCAGATAAAATACACAGTAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGCGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGGTGAGTCTGTCTCTCCTTTATGCCATTGTTGTCACTCTGTGGCTTTTGTCATCTTTGTGGCTATGGTTAAATTGCTCTATTCCTTGCAGTTGTGTTCAAATACGGAACTGGTTTGACTATCGTAACCATATTTGTATTGTAAGTATAAGGCTGTTTTATCATGGATTAATTTCCCCGATTCACCAAATATGGGGAGTTAAAATTTGTGGGTGAACTTAAATGGTGTCAACAGGTGTTTGAGAAGCTTGGGCCAAGCTTATACGATTTTCTACGGAAAAACAATTATCGCTCCTTTCCCATTGACCTAGTTCGTGACATTGGCAGACAACTGTTGGAATGTGTAGCATGTGTGTAGTATTTTATTGAACCAAGTCCTTTCTTTTCCCATTCTTATTGTCCTTTTGTGTGTCACAAAACATTAACTCCACAGATTTCTTTAAGCTAAAATGATTATTTGCATCCGCTTCATGTTCCATTTTTGTAATTTCATTTAAATGGTATGATGAGTCGGTCTCCTTTTATTTTGTGTCTTGTATCCTAATAATTTCTTTGACCCTTGAACTGCAGTCATGCATGATCTACGAATGATACATACTGACTTGAAGCCAGAGAACATACTGTTGGTCTCTGCAGATTATATGAAAGTACATGACTACAAGGTACTTATAGCTTGTTCTGGATCTTGTTTGTAATCTGTATTTCTGGTGGTTCTTATTGTGAAAGACCAATTACTAGCTAAATGATGTTCTTGTTACTCATTATTTATACTTGTGTCAGAATTTTTCTCGATCACCAAGGGATAGTTCCAACTTCAAAAGAGTGCCCAAGTCAAGTTCTATTAAGGTGATTGATTTTGGCAGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCAACCCGGCATTATAGAGCTCCAGAGGTTATTCTTGGTAAGGAATTTTGATCATGTTTCATAGTTCACTTTTCGATTGTTCTGATTTGTGTTGCGTGGCATGACTTAACCACGTTTATCGTTTAGGTATTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGTTGTATCCTTGTCGAACTGTGCTCGGTATGTGCTTCTTTTGTCTTGTAATTTGTCTATTTCAATATATTCTTCTTTTGTTTATTCATCCAAGTGTTGTATTGTGTAATTGAATTTTCAATACGAAATAATTTTTAGGGTGAAGCACTGTTTCAAACTCATGAGAACTTGGAGCACTTGGCTATGATGGAGCGGGTGCTTGGTCCATTGCCGCAAGAGATGCTGAAACGAGTAGAGTGAGTTATTTCTTATCTTTTATTTTCTTGGTTTTAAGTAGTGAAGAAGCAAAATTCTGATTATTTTGTTTGTTTGAATGTGTCCATAGTCGGCATGCTGAAAAATACGTAAGAAGAGGTAGATTAGATTGGCCGGAAGGTGCTACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGCTTCGTTTACCGGTTTGTATTTTTAGTAGTGGCTTATCGGTTTGATTGGACTGAGCCTTCTTGTCGCAACTTTAGACTAATTATCTTGTGAAATCGAATGCAGAACTTGATAATGCAGCATGTGGACCATTCTGCTGGGGAATTGATTCATCTCGTGCAAGGGTTGCTTCGATATGATCCCACGCATAGACTAACGGCTCGTGAAGCCCTAAGGCACCCCTTCTTTACAAGGGATCCCTTGAGGAGATAGTAGCTGTGCTGTGTTTAATTAACCAACTGGTATTTGGGTTTCTTTTTAAACACTCACAACGTCTTCATGCAACTGTACAGCTGCGTATCTGTTGATCGATGATCGTGATGTGCCTTTGTATGGCTCTAAGAGACCGAGTATTTTGCCGTGATGTTGTAAATAGATACGCTTACTCCTATCAGAGGCTTGCAATGCAAGCACTATTTGTGCTTAATTGCAGTTCAGTTGATTACTAATGTTTTAACTTGTTTAGAAGAATATACACTAACATTTTATGACATTTGCTGTCCATTTCTTCAAGATTATCATAAAAAACTATTGGAATTGTGATACATAATATTATAGGGTGATGATGTTATTAATTTTTATGAGTTACTATCGTGAAAATGTATCCTAGAAACGTTAATAGAAACTTATA

mRNA sequence

TCTCCGCAACGTGGACGGTTGGATTTCCTCTCATCCTCCATATGCTTCGTTGTTAGGTTAAAATTAGGGTTGCGTTTCGATTCCATTCCACAAATTTCTTTCTGCGGAAGAATATTTCTTCTCATCTCCCTCTTCAAAGCCCCCGGAAATCTGGTTCTTCAATTCTCTTCTTCTTTTCAAGGCTCTGCTTCGTTGTGCGAGATCATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTCGATCGCAGGCCCCGAAAGAGGGCTCGCTTGGGCTGGGACGTTGTCCCCGAGGCCGCTAAGGCTCAGGTAGGAATTTGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCACGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGACATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACACAGTAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGCGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCAACCCGGCATTATAGAGCTCCAGAGGTTATTCTTGGTATTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGTTGTATCCTTGTCGAACTGTGCTCGGGTGAAGCACTGTTTCAAACTCATGAGAACTTGGAGCACTTGGCTATGATGGAGCGGGTGCTTGGTCCATTGCCGCAAGAGATGCTGAAACGAGTAGATCGGCATGCTGAAAAATACGTAAGAAGAGGTAGATTAGATTGGCCGGAAGGTGCTACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGCTTCGTTTACCGAACTTGATAATGCAGCATGTGGACCATTCTGCTGGGGAATTGATTCATCTCGTGCAAGGGTTGCTTCGATATGATCCCACGCATAGACTAACGGCTCGTGAAGCCCTAAGGCACCCCTTCTTTACAAGGGATCCCTTGAGGAGATAGTAGCTGTGCTGTGTTTAATTAACCAACTGGTATTTGGGTTTCTTTTTAAACACTCACAACGTCTTCATGCAACTGTACAGCTGCGTATCTGTTGATCGATGATCGTGATGTGCCTTTGTATGGCTCTAAGAGACCGAGTATTTTGCCGTGATGTTGTAAATAGATACGCTTACTCCTATCAGAGGCTTGCAATGCAAGCACTATTTGTGCTTAATTGCAGTTCAGTTGATTACTAATGTTTTAACTTGTTTAGAAGAATATACACTAACATTTTATGACATTTGCTGTCCATTTCTTCAAGATTATCATAAAAAACTATTGGAATTGTGATACATAATATTATAGGGTGATGATGTTATTAATTTTTATGAGTTACTATCGTGAAAATGTATCCTAGAAACGTTAATAGAAACTTATA

Coding sequence (CDS)

ATGGAGATGGAGCGCATGATCGAGTTTCCTCACACACATCTCGATCGCAGGCCCCGAAAGAGGGCTCGCTTGGGCTGGGACGTTGTCCCCGAGGCCGCTAAGGCTCAGGTAGGAATTTGTTGTGGACAAGAGATTGCGAATATGCCAAGCTTTGCATCTTCAAGAGCACCTTCAGATCATTCTTCTAATCCACTATTTGTTAAGGGAGTGGCACGAAATGGTTCTCCCCCCTGGCGAGAAGATGACAAAGATGGACATTACATGTTTGCGCTTGGAGAAAATTTAACTTCTCGCTATAAAATACACAGTAAAATGGGAGAAGGTACTTTTGGACAGGTGTTGGAATGCTGGGACAGAGAAAGAAAGGAAATGGTTGCAATAAAAATTGTGCGCGGAATCAGGAAATATCGTGATGCAGCTATGATAGAAATTGAAGTGTTGCAACAGCTTGGAAAGCATGATAAAGGTGGCAACCGCACCACTTATGATCGTCAAGATCAGAATTACATTGTATCAACCCGGCATTATAGAGCTCCAGAGGTTATTCTTGGTATTGGATGGAGTTTTCCTTGTGATATATGGAGTGTTGGTTGTATCCTTGTCGAACTGTGCTCGGGTGAAGCACTGTTTCAAACTCATGAGAACTTGGAGCACTTGGCTATGATGGAGCGGGTGCTTGGTCCATTGCCGCAAGAGATGCTGAAACGAGTAGATCGGCATGCTGAAAAATACGTAAGAAGAGGTAGATTAGATTGGCCGGAAGGTGCTACTTCGAGGGATAGTATCAAGGCCGTTCAAAAGCTGCTTCGTTTACCGAACTTGATAATGCAGCATGTGGACCATTCTGCTGGGGAATTGATTCATCTCGTGCAAGGGTTGCTTCGATATGATCCCACGCATAGACTAACGGCTCGTGAAGCCCTAAGGCACCCCTTCTTTACAAGGGATCCCTTGAGGAGATAG

Protein sequence

MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRTTYDRQDQNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGRLDWPEGATSRDSIKAVQKLLRLPNLIMQHVDHSAGELIHLVQGLLRYDPTHRLTAREALRHPFFTRDPLRR

Homology

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BLAST of Cp4.1LG20g03420 vs. ExPASy Swiss-Prot
Match: P51567 (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 3.2e-146
Identity = 265/426 (62.21%), Postives = 298/426 (69.95%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEI N+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RYKI+SKMGEGTFGQVLECWDRE
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 ------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGWSF 300
                                         TTY+RQDQ YIVSTRHYRAPEVILG+GWS+
Sbjct: 241 PEYKGSRLQRDVCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWSY 300

Query: 301 PCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGR 316
           PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVLGP PQ+MLK+VDRH+EKYVRRGR
Sbjct: 301 PCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRGR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy Swiss-Prot
Match: P51566 (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=2 SV=2)

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-109
Identity = 216/430 (50.23%), Postives = 252/430 (58.60%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR-------------------- 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+R                    
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 ---------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIG 300
                                            TT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 316
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy Swiss-Prot
Match: P51568 (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=1 SV=2)

HSP 1 Score: 304.3 bits (778), Expect = 1.6e-81
Identity = 167/385 (43.38%), Postives = 210/385 (54.55%), Query Frame = 0


Query: 43  QEIANMPSFASSRAPSDHSSNPLFVKGVARNG---SPPWREDDKDGHYMFALGENLTSRY 102
           + +   P      AP++  +    +KG   +G   SPP R+DD+DGHY+F+L +NLT RY
Sbjct: 12  ERVRKRPRMTWDEAPAEPEAKRAVIKGHGSDGRILSPPLRDDDRDGHYVFSLRDNLTPRY 71

Query: 103 KIHSKMGEGTFGQVLECWDRERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR 162
           KI SKMGEGTFG+VLECWDR+ KE VAIKI+R I+KYRDAAMIEI+VLQ+L K DKG  R
Sbjct: 72  KILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTR 131

Query: 163 ------------------------------------------------------------ 222
                                                                       
Sbjct: 132 CVQMKNWFDYRNHICIVFEKLGPSLFDFLKRNKYSAFPLALVRDFGCQLLESVAYMHELQ 191

Query: 223 --------------------------------------------------TTYDRQDQNY 282
                                                             T  D +  + 
Sbjct: 192 LVHTDLKPENILLVSSENVKLPDNKRSAANETHFRCLPKSSAIKLIDFGSTVCDNRIHHS 251

Query: 283 IVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPL 314
           IV TRHYR+PEVILG+GWS+ CD+WS+GCIL ELC+GEALFQTH+NLEHLAMMER LGPL
Sbjct: 252 IVQTRHYRSPEVILGLGWSYQCDLWSIGCILFELCTGEALFQTHDNLEHLAMMERALGPL 311

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy Swiss-Prot
Match: P49760 (Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.5e-46
Identity = 122/369 (33.06%), Postives = 171/369 (46.34%), Query Frame = 0


Query: 54  SRAPSDHSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQV 113
           SR+ S HSS     K V         EDD +GH ++ +G+ L  RY+I S +GEGTFG+V
Sbjct: 129 SRSSSQHSSRR--AKSV---------EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRV 188

Query: 114 LECWDRER-KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHD------------------ 173
           ++C D  R    VA+KI++ + KY++AA +EI VL+++ + D                  
Sbjct: 189 VQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHG 248

Query: 174 -----------------KGGN--------------------------------------- 233
                            K  N                                       
Sbjct: 249 HMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENIL 308

Query: 234 --------------------------------RTTYDRQDQNYIVSTRHYRAPEVILGIG 293
                                             T+D +  + IVSTRHYRAPEVIL +G
Sbjct: 309 FVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELG 368

Query: 294 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 316
           WS PCD+WS+GCI+ E   G  LFQTH+N EHLAMMER+LGP+P  M+++  +  +KY  
Sbjct: 369 WSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRK--QKYFY 428

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy Swiss-Prot
Match: O35491 (Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 114/341 (33.43%), Postives = 161/341 (47.21%), Query Frame = 0


Query: 80  EDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRER-KEMVAIKIVRGIRKYRD 139
           EDD +GH ++ +G+ L  RY+I S +GEGTFG+V++C D  R    VA+KI++ + KY++
Sbjct: 143 EDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKE 202

Query: 140 AAMIEIEVLQQLGKHD-----------------------------------KGGN----- 199
           AA +EI VL+++ + D                                   K  N     
Sbjct: 203 AARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYP 262

Query: 200 ------------------------------------------------------------ 259
                                                                       
Sbjct: 263 IHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAV 322

Query: 260 ------RTTYDRQDQNYIVSTRHYRAPEVILGIGWSFPCDIWSVGCILVELCSGEALFQT 314
                   T+D +  + IVSTRHYRAPEVIL +GWS PCD+WS+GCI+ E   G  LFQT
Sbjct: 323 RVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQT 382

[Close]

BLAST of Cp4.1LG20g03420 vs. NCBI nr
Match: XP_023001260.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 617 bits (1591), Expect = 1.30e-220
Identity = 320/433 (73.90%), Postives = 320/433 (73.90%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. NCBI nr
Match: XP_023519374.1 (serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617 bits (1591), Expect = 1.30e-220
Identity = 320/433 (73.90%), Postives = 320/433 (73.90%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. NCBI nr
Match: XP_022923920.1 (serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116.1 Serine/threonine-protein kinase AFC2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019705.1 Serine/threonine-protein kinase AFC2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 616 bits (1588), Expect = 3.71e-220
Identity = 319/433 (73.67%), Postives = 320/433 (73.90%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDR+DQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDREDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. NCBI nr
Match: XP_038894261.1 (serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida])

HSP 1 Score: 604 bits (1557), Expect = 1.95e-215
Identity = 312/433 (72.06%), Postives = 316/433 (72.98%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           +KE VAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 KKETVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPRSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. NCBI nr
Match: XP_008439081.1 (PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA0055279.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa] >TYJ99202.1 serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 603 bits (1555), Expect = 3.93e-215
Identity = 310/433 (71.59%), Postives = 317/433 (73.21%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy TrEMBL
Match: A0A6J1KKP8 (serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495446 PE=4 SV=1)

HSP 1 Score: 617 bits (1591), Expect = 6.28e-221
Identity = 320/433 (73.90%), Postives = 320/433 (73.90%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSFIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy TrEMBL
Match: A0A6J1EDB4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431493 PE=4 SV=1)

HSP 1 Score: 616 bits (1588), Expect = 1.80e-220
Identity = 319/433 (73.67%), Postives = 320/433 (73.90%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSADYMKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDR+DQNYIVSTRHYRAPEVILGIGW
Sbjct: 241 HDYKNFSRSPRDSSNFKRVPKSSSIKVIDFGSTTYDREDQNYIVSTRHYRAPEVILGIGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy TrEMBL
Match: A0A5A7UP87 (Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004120 PE=4 SV=1)

HSP 1 Score: 603 bits (1555), Expect = 1.90e-215
Identity = 310/433 (71.59%), Postives = 317/433 (73.21%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy TrEMBL
Match: A0A1S3AXH4 (serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483972 PE=4 SV=1)

HSP 1 Score: 603 bits (1555), Expect = 1.90e-215
Identity = 310/433 (71.59%), Postives = 317/433 (73.21%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIAN+PSFAS+RAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANIPSFASTRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALG+NLTSRYKIHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGDNLTSRYKIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           +KEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 KKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIARQLLECVAFMHDLRMIHTDLKPENILLVSGDYVKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSSNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQ+MLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQDMLKKVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. ExPASy TrEMBL
Match: A0A6J1C5J1 (serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008590 PE=4 SV=1)

HSP 1 Score: 597 bits (1538), Expect = 7.37e-213
Identity = 309/433 (71.36%), Postives = 315/433 (72.75%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMERMIEFPHTHLDRRPRKRARLGWDVVPEA KAQVGICCGQEIANMPSFASSRAPSDH
Sbjct: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAPKAQVGICCGQEIANMPSFASSRAPSDH 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRY+IHSKMGEGTFGQVLECWDRE
Sbjct: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYRIHSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GPSLYDFLRKNNYRSFPIDLVRDIGRQLLECVAFMHDLRMIHTDLKPENILLVSVDYVKV 240

Query: 241 --------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGW 300
                                           TTYDRQDQNYIVSTRHYRAPEVILG+GW
Sbjct: 241 HDYKNLSRSPRDSTNFKRVPKSSAIKVIDFGSTTYDRQDQNYIVSTRHYRAPEVILGLGW 300

Query: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRR 320
           SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLK+VDRHAEKYVRR
Sbjct: 301 SFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKKVDRHAEKYVRR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. TAIR 10
Match: AT4G24740.1 (FUS3-complementing gene 2 )

HSP 1 Score: 519.2 bits (1336), Expect = 2.3e-147
Identity = 265/426 (62.21%), Postives = 298/426 (69.95%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQEIANMPSFASSRAPSDH 60
           MEMER+ EFPHTH+DRRPRKRARLGWDV+P+A KAQVG+ CGQEI N+ SFASS APSD+
Sbjct: 1   MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQVGMFCGQEIGNISSFASSGAPSDN 60

Query: 61  SSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDRE 120
           SS+ L VKGVARNGSPPWREDDKDGHY+F LG++LT RYKI+SKMGEGTFGQVLECWDRE
Sbjct: 61  SSS-LCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRE 120

Query: 121 RKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR--------------------- 180
           RKEMVA+KIVRG++KYR+AAMIEIE+LQQLGKHDKGGNR                     
Sbjct: 121 RKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIVFEKL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 GSSLYDFLRKNNYRSFPIDLVREIGWQLLECVAFMHDLRMIHTDLKPENILLVSSDYVKI 240

Query: 241 ------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIGWSF 300
                                         TTY+RQDQ YIVSTRHYRAPEVILG+GWS+
Sbjct: 241 PEYKGSRLQRDVCYKRVPKSSAIKVIDFGSTTYERQDQTYIVSTRHYRAPEVILGLGWSY 300

Query: 301 PCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVRRGR 316
           PCD+WSVGCI+VELC+GEALFQTHENLEHLAMMERVLGP PQ+MLK+VDRH+EKYVRRGR
Sbjct: 301 PCDVWSVGCIIVELCTGEALFQTHENLEHLAMMERVLGPFPQQMLKKVDRHSEKYVRRGR 360

[Close]

BLAST of Cp4.1LG20g03420 vs. TAIR 10
Match: AT3G53570.3 (FUS3-complementing gene 1 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.0e-110
Identity = 216/430 (50.23%), Postives = 252/430 (58.60%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FAS   P+ 
Sbjct: 3   LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 62

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 63  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 122

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR-------------------- 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+R                    
Sbjct: 123 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 182

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 183 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 242

Query: 241 ---------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIG 300
                                            TT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 243 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 302

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 316
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 303 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 362

[Close]

BLAST of Cp4.1LG20g03420 vs. TAIR 10
Match: AT3G53570.1 (FUS3-complementing gene 1 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.0e-110
Identity = 216/430 (50.23%), Postives = 252/430 (58.60%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR-------------------- 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+R                    
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 ---------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIG 300
                                            TT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 316
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

[Close]

BLAST of Cp4.1LG20g03420 vs. TAIR 10
Match: AT3G53570.2 (FUS3-complementing gene 1 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.0e-110
Identity = 216/430 (50.23%), Postives = 252/430 (58.60%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR-------------------- 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+R                    
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 ---------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIG 300
                                            TT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 316
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

[Close]

BLAST of Cp4.1LG20g03420 vs. TAIR 10
Match: AT3G53570.4 (FUS3-complementing gene 1 )

HSP 1 Score: 397.5 bits (1020), Expect = 1.0e-110
Identity = 216/430 (50.23%), Postives = 252/430 (58.60%), Query Frame = 0


Query: 1   MEMERMIEFPHTHLDRRPRKRARLGWDVVPEAAKAQVGICCGQ-EIANMPSFASSRAPSD 60
           +E +R +EFPH  +D+RPRKR RL WD  P            Q  +   P FAS   P+ 
Sbjct: 17  LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNF 76

Query: 61  HSSNPLFVKGVARNGSPPWREDDKDGHYMFALGENLTSRYKIHSKMGEGTFGQVLECWDR 120
              N +F  G+ R GSPPWR DDKDGHY+F +G+ LT RY+I SKMGEGTFGQVLEC+D 
Sbjct: 77  VYPN-MFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDN 136

Query: 121 ERKEMVAIKIVRGIRKYRDAAMIEIEVLQQLGKHDKGGNR-------------------- 180
           + KE+VAIK++R I KYR+AAMIEI+VLQ+L +HD GG+R                    
Sbjct: 137 KNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIVFEK 196

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 197 LGPSLYDFLRKNSYRSFPIDLVRELGRQLLESVAYMHDLRLIHTDLKPENILLVSSEYIK 256

Query: 241 ---------------------------------TTYDRQDQNYIVSTRHYRAPEVILGIG 300
                                            TT++ QD NYIVSTRHYRAPEVILG+G
Sbjct: 257 IPDYKFLSRPTKDGSYFKNLPKSSAIKLIDFGSTTFEHQDHNYIVSTRHYRAPEVILGVG 316

Query: 301 WSFPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGPLPQEMLKRVDRHAEKYVR 316
           W++PCD+WS+GCILVELCSGEALFQTHENLEHLAMMERVLGPLP  M+ R DR +EKY R
Sbjct: 317 WNYPCDLWSIGCILVELCSGEALFQTHENLEHLAMMERVLGPLPPHMVLRADRRSEKYFR 376

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g03420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51567	3.2e-146	62.21	Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana OX=3702 GN=AFC2 PE=...	[more]
P51566	1.4e-109	50.23	Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana OX=3702 GN=AFC1 PE=...	[more]
P51568	1.6e-81	43.38	Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana OX=3702 GN=AFC3 PE=...	[more]
P49760	6.5e-46	33.06	Dual specificity protein kinase CLK2 OS=Homo sapiens OX=9606 GN=CLK2 PE=1 SV=1	[more]
O35491	1.1e-45	33.43	Dual specificity protein kinase CLK2 OS=Mus musculus OX=10090 GN=Clk2 PE=1 SV=2	[more]

BLAST of Cp4.1LG20g03420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023001260.1	1.30e-220	73.90	serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita maxima]	[more]
XP_023519374.1	1.30e-220	73.90	serine/threonine-protein kinase AFC2-like isoform X1 [Cucurbita pepo subsp. pepo...	[more]
XP_022923920.1	3.71e-220	73.67	serine/threonine-protein kinase AFC2 isoform X1 [Cucurbita moschata] >KAG6584116...	[more]
XP_038894261.1	1.95e-215	72.06	serine/threonine-protein kinase AFC2 isoform X1 [Benincasa hispida]	[more]
XP_008439081.1	3.93e-215	71.59	PREDICTED: serine/threonine-protein kinase AFC2 isoform X1 [Cucumis melo] >KAA00...	[more]

BLAST of Cp4.1LG20g03420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KKP8	6.28e-221	73.90	serine/threonine-protein kinase AFC2-like isoform X1 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EDB4	1.80e-220	73.67	serine/threonine-protein kinase AFC2 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5A7UP87	1.90e-215	71.59	Serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3AXH4	1.90e-215	71.59	serine/threonine-protein kinase AFC2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1C5J1	7.37e-213	71.36	serine/threonine-protein kinase AFC2 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Cp4.1LG20g03420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24740.1	2.3e-147	62.21	FUS3-complementing gene 2	[more]
AT3G53570.3	1.0e-110	50.23	FUS3-complementing gene 1	[more]
AT3G53570.1	1.0e-110	50.23	FUS3-complementing gene 1	[more]
AT3G53570.2	1.0e-110	50.23	FUS3-complementing gene 1	[more]
AT3G53570.4	1.0e-110	50.23	FUS3-complementing gene 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 99..313 e-value: 2.3E-13 score: 57.9
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 163..313 e-value: 1.1E-19 score: 70.8
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..313 score: 14.661919
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 72..154 e-value: 1.8E-21 score: 77.5
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 161..313 e-value: 4.6E-45 score: 156.2
None	No IPR available	PANTHER	PTHR45646	SERINE/THREONINE-PROTEIN KINASE DOA-RELATED	coord: 1..160
None	No IPR available	PANTHER	PTHR45646	SERINE/THREONINE-PROTEIN KINASE DOA-RELATED	coord: 160..320
None	No IPR available	PANTHER	PTHR45646:SF16	KINASE AFC1	coord: 160..320 coord: 1..160
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 84..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g03420.1	Cp4.1LG20g03420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity