Cp4.1LG20g03280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g03280 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 7.9e-71
Identity = 162/423 (38.30%), Postives = 241/423 (56.97%), Query Frame = 0

Query: 8   VEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCRKL 67
           ++I+S E I+PSSPTP   +  KLS LDQV  + + P++ FY  +QL    D  +R   L
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFY-PNQLDSNLDRAQRSENL 64

Query: 68  KESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRLLP 127
           K SL+  L +FY LAG +     VDCND GV F EARV  ++SE + K   +  L + LP
Sbjct: 65  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAI-KNVAIDELNQYLP 124

Query: 128 FD-----HDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGD 187
           F       ++ L+++  LAV+ + FECGG  + +C++HKI D  S   F  +W A C+ +
Sbjct: 125 FQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQ-E 184

Query: 188 DKYPVVPTFD-ATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFLN 247
           +   V P FD  +  FPPME           + IV +RF+F+K  + ALK Q  S A   
Sbjct: 185 ETDIVQPNFDLGSHHFPPMESIPAPEFLPD-ENIVMKRFVFDKEKLEALKAQLASSA-TE 244

Query: 248 QRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWWF 307
            +  SRV+ V   +WK+F+ + + K  TK K  A  QAVNLR+RMNPP P  + GN+   
Sbjct: 245 VKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAA-QAVNLRSRMNPPFPQSAMGNIATM 304

Query: 308 ATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGE-LEL 367
           A A A  +E++DF  LV  ++ ++ +I+ +++K LQ           +  + Y  E  EL
Sbjct: 305 AYAVA--EEDKDFSDLVGPLKTSLAKIDDEHVKELQ---------KGVTYLDYEAEPQEL 364

Query: 368 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 424
           +SF+SWC    Y+ DFGWGKP  VC+   P KN+V  ++T + +G+EAW+++ E +M   
Sbjct: 365 FSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDEMSML 410

BLAST of Cp4.1LG20g03280 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 1.5e-66
Identity = 151/419 (36.04%), Postives = 231/419 (55.13%), Query Frame = 0

Query: 8   VEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCRKL 67
           +E +S E I PSSPTP   +  K+S LDQ+  + + P + FY  + L    D  +  + L
Sbjct: 5   MEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFY-PNPLDSNLDPAQTSQHL 64

Query: 68  KESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRLLP 127
           K+SL++ L  FY LAG +     VDCND GV F EARV  ++S+ ++   ++  L + LP
Sbjct: 65  KQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLP 124

Query: 128 F----DHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDD 187
                     +  +  LAV+ + FECGG  + + ++HKI D  S   F  AW A CRG+ 
Sbjct: 125 SAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGET 184

Query: 188 KYPVVPTFD-ATELFPPMEIRGGNRRQSRM---DKIVTRRFIFNKSNIAALKNQANSEAF 247
           +  V+P FD A   FPP++    N     +   + +V +RF+F+K  I AL+ QA+S + 
Sbjct: 185 EI-VLPNFDLAARHFPPVD----NTPSPELVPDENVVMKRFVFDKEKIGALRAQASSAS- 244

Query: 248 LNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLW 307
             ++  SRV+ V  ++WK  + + + K   K K F V+QAVNLR+RMNPPLP ++ GN+ 
Sbjct: 245 -EEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNK-FVVVQAVNLRSRMNPPLPHYAMGNIA 304

Query: 308 WFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGE-L 367
               A    + ++DFP L+  +R ++++   D+   L          +K    +Y  E  
Sbjct: 305 TLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDDHNHEL----------LKGMTCLYELEPQ 364

Query: 368 ELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDM 418
           EL SFTSWC    Y+ DFGWGKP   C+   P +N  L ++T  G+G+EAW+ + E +M
Sbjct: 365 ELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAEDEM 404

BLAST of Cp4.1LG20g03280 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.7e-63
Identity = 161/467 (34.48%), Postives = 233/467 (49.89%), Query Frame = 0

Query: 3   MNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTA--DQLHGGDDH 62
           M++  VE+IS E+IKP +PTP + R   +S LDQ +   Y P++ FY A  D    G  H
Sbjct: 4   MSSAAVEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKH 63

Query: 63  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 122
            +    LK SL+ETL  FY +AG + +   VDCND+G+DF E R+ GRM + M K    +
Sbjct: 64  HDDLHLLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDAHL 123

Query: 123 SLLRLLPFDHDAV-LERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAA-- 182
           SL  LLP +  +    +E  + V+ N+F+CGG  +  C+++KI D  +   F ++ A   
Sbjct: 124 SL--LLPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTT 183

Query: 183 --ACRGDD------KYPVVPTFDATELFPPME-----IRGGNRRQSRMDKIVTRRFIFNK 242
             A RG           +VP+FD+T LFPP E     +    +  + +DK+V++RF+F+ 
Sbjct: 184 NIARRGSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSVDKLVSKRFVFDA 243

Query: 243 SNIAALKNQANS---EAFLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVN 302
           + I + + +  S   + +   R P+RVE VS  +WK   +  +  PP       V  A+N
Sbjct: 244 AKITSAREKLQSLMHDKYKCHR-PTRVEVVSALIWK---SAVKSAPPGSIS--TVTHAMN 303

Query: 303 LRNRMNPPLPPHSFGNLWWFATADAP------------------------IDEEQDFPSL 362
            R +M+PPL   SFGNL    TA  P                        +DE  DF +L
Sbjct: 304 FRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDFVAL 363

Query: 363 VAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELELYSFTSWCSFPLYESDFG 422
           + +  + +K   G   K +Q       A +     V      L+  TSWC F  YE+DFG
Sbjct: 364 LRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALF-MTSWCKFGFYEADFG 423

BLAST of Cp4.1LG20g03280 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 2.2e-60
Identity = 153/430 (35.58%), Postives = 236/430 (54.88%), Query Frame = 0

Query: 8   VEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCRKL 67
           VEI+S   IKPSSPTP   R   LS +DQ   S  T L FFYT +  H      +    L
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFYTYNPSHESSKIGD---LL 65

Query: 68  KESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRLLP 127
           K SL++TL  +Y  AG L+E   +DCND+GV+F E R+ GRM++++++     + L +LP
Sbjct: 66  KNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDL-VLP 125

Query: 128 FDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKYPV 187
                 L  + +L V+ + F+CGG+ +    +HK+ DG S  MF K WA++      +  
Sbjct: 126 -TRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKP 185

Query: 188 VPTFDATELFPPMEIR-GGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFLNQRPPS 247
            P   A  +FPP + +   N+      + + +RF+F+   I  LK++A        + PS
Sbjct: 186 TPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYGI---QKPS 245

Query: 248 RVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNL--WWFATA 307
           RVE V+ FL +     A    P K K +A+I AVNLR  +   LP +S GN+  ++F   
Sbjct: 246 RVEVVTAFLCQ---CAANCDLPRK-KPYAIISAVNLRPYL--ALPQNSIGNIFSFYFCIN 305

Query: 308 DAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDT-EKSMKAKMKMGQMVYSGELELYSF 367
           D  +D +  F +L++K+R   +++  + I   + T E  M+   K    +    L+ Y  
Sbjct: 306 DEGMDNQ--FSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQLNISSLDTYFC 365

Query: 368 TSWCSFPLYESDFGWGKPAWVCSPGRPY-KNVVLFVNTSDGEGIEAWVNLTETDMKDFET 427
           +SWC FP+Y+ DFGWGKP  V SP +P+ K+++L +++ +G+GIEA + + E  M  FE 
Sbjct: 366 SSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITMEEKKMAAFEK 418

Query: 428 DSELLSFASL 433
           + EL SFA L
Sbjct: 426 NEELRSFAYL 418

BLAST of Cp4.1LG20g03280 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.2e-60
Identity = 161/479 (33.61%), Postives = 239/479 (49.90%), Query Frame = 0

Query: 3   MNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAP-SSYTPLLFFY--TADQLHGGDD 62
           M +  VE+IS E+IKP++PTPS+ +   LS LDQ  P   Y P++ FY  TA    G  +
Sbjct: 4   MYSAAVEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSN 63

Query: 63  HRERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDV 122
           H +    LK SL++TL  FY +AG +++  LVDC+D G++F + ++ G+M E M +P   
Sbjct: 64  HHDDLDLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQPD-- 123

Query: 123 VSLLRLLPFD-HDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAA 182
           V L +LLP +   A + +E ++ V+ N+F+CGG  +   V+HKI D  + + F ++WA+ 
Sbjct: 124 VPLSQLLPSEVVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWAST 183

Query: 183 CRGD---------DKYPVVPTFDATELFPPMEIRGGNRRQSRM-----------DKIVTR 242
            +               ++P+FD+  LFPP E        S +           DK V++
Sbjct: 184 TKTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSK 243

Query: 243 RFIFNKSNIAAL--KNQANSEAFLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAV 302
           RF+F+ + I ++  K Q         R  +RVE V+  +WK  M        T A    V
Sbjct: 244 RFVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVM------KSTPAGFLPV 303

Query: 303 I-QAVNLRNRMNPPLPPHSFGNLWWFATADAPI-----------------DEEQ----DF 362
           +  AVNLR +M+PPL   SFGNL    +A  P                   E Q    + 
Sbjct: 304 VHHAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHEL 363

Query: 363 PSLVAKIREAIKEINGD---YIKTLQDTEKSMKAKMKMGQMVYSGELELYSFTSWCSFPL 422
              +A++R  I ++ GD     K +Q+      A +K    +   E+  +  +SWC   L
Sbjct: 364 HDFIAQMRSEIDKVKGDKGSLEKVIQNFASGHDASIK---KINDVEVINFWISSWCRMGL 423

Query: 423 YESDFGWGKPAWV-CSPG-RPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETD-SELL 428
           YE DFGWGKP WV   P  +P KN     +T  GEGIE W +  E DM  FE   SE+L
Sbjct: 424 YEIDFGWGKPIWVTVDPNIKPNKNCFFMNDTKCGEGIEVWASFLEDDMAKFELHLSEIL 471

BLAST of Cp4.1LG20g03280 vs. NCBI nr
Match: XP_023520398.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 876 bits (2264), Expect = 0.0
Identity = 433/433 (100.00%), Postives = 433/433 (100.00%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH
Sbjct: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120
           RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV
Sbjct: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120

Query: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180
           SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR
Sbjct: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180

Query: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240
           GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL
Sbjct: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240

Query: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW 300
           NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW
Sbjct: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW 300

Query: 301 FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL 360
           FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL
Sbjct: 301 FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL 360

Query: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420
           YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF
Sbjct: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420

Query: 421 ETDSELLSFASLS 433
           ETDSELLSFASLS
Sbjct: 421 ETDSELLSFASLS 433

BLAST of Cp4.1LG20g03280 vs. NCBI nr
Match: XP_022923632.1 (vinorine synthase-like [Cucurbita moschata] >KAG6584134.1 Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019724.1 Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863 bits (2229), Expect = 0.0
Identity = 425/433 (98.15%), Postives = 433/433 (100.00%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH
Sbjct: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120
           RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGV+FSEARVSGRMSEVMEKPHDVV
Sbjct: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSEARVSGRMSEVMEKPHDVV 120

Query: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180
           SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR
Sbjct: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180

Query: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240
           GDDKYPVVPTFDATELFPPMEIRGGNRRQSRM+KIVTRRFIFNKSNIAALK+QANSEAFL
Sbjct: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMEKIVTRRFIFNKSNIAALKHQANSEAFL 240

Query: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW 300
           NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRM+PPLPP+SFGNLWW
Sbjct: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMDPPLPPNSFGNLWW 300

Query: 301 FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL 360
           FATADAPIDEEQDFPSLVAKIREAIKEI+GDYIKT+QDTEKSMKAKMKMGQMVYSGELEL
Sbjct: 301 FATADAPIDEEQDFPSLVAKIREAIKEIDGDYIKTIQDTEKSMKAKMKMGQMVYSGELEL 360

Query: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420
           YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF
Sbjct: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420

Query: 421 ETDSELLSFASLS 433
           ETD+ELLSFASLS
Sbjct: 421 ETDAELLSFASLS 433

BLAST of Cp4.1LG20g03280 vs. NCBI nr
Match: XP_023000804.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 860 bits (2223), Expect = 7.10e-315
Identity = 425/433 (98.15%), Postives = 430/433 (99.31%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH
Sbjct: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120
           RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGV+FSE RVS RMSEVMEKPHDVV
Sbjct: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSETRVSARMSEVMEKPHDVV 120

Query: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180
           SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR
Sbjct: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180

Query: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240
           GDDKYPVVPTF+ATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL
Sbjct: 181 GDDKYPVVPTFNATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240

Query: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW 300
           NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRM+PPLPP+SFGNLWW
Sbjct: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMDPPLPPNSFGNLWW 300

Query: 301 FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL 360
           FATADAPIDEEQDFPSLVAKIREAIKEI+GDYIKTLQD EKSMKAKMKMGQMVYSGELEL
Sbjct: 301 FATADAPIDEEQDFPSLVAKIREAIKEIDGDYIKTLQDAEKSMKAKMKMGQMVYSGELEL 360

Query: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420
           YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF
Sbjct: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420

Query: 421 ETDSELLSFASLS 433
           ETDSELLSFASLS
Sbjct: 421 ETDSELLSFASLS 433

BLAST of Cp4.1LG20g03280 vs. NCBI nr
Match: XP_038895261.1 (LOW QUALITY PROTEIN: stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 741 bits (1912), Expect = 2.09e-267
Identity = 368/441 (83.45%), Postives = 393/441 (89.12%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           MEMNNL VEIISTE IKPSSPTPS+H++ KLS LDQ APSSYTPLLFFY ADQLHG DDH
Sbjct: 1   MEMNNLKVEIISTEIIKPSSPTPSDHQQHKLSLLDQFAPSSYTPLLFFYPADQLHGADDH 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120
           R+RCRKLKESLAETL+RFYLLAGTLVEAYLV+CNDDGV F+EARVS R+SEVME PHDVV
Sbjct: 61  RDRCRKLKESLAETLSRFYLLAGTLVEAYLVECNDDGVAFTEARVSRRLSEVMENPHDVV 120

Query: 121 SLLRLLPFDHDAVL-------ERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTK 180
            L RLLPF  DAVL       ERECILAVRYNVFECGGVV+ALCVTHKIVDGTSAT+FTK
Sbjct: 121 LLHRLLPFQPDAVLXXXXAVLERECILAVRYNVFECGGVVIALCVTHKIVDGTSATLFTK 180

Query: 181 AWAAACRGDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQ 240
           AWAA CRGD++YPVVPTF+A ELFPPME  GGN+RQSRM KIVTRRFIFNKSNIAALK Q
Sbjct: 181 AWAATCRGDNEYPVVPTFNAAELFPPMENHGGNKRQSRMQKIVTRRFIFNKSNIAALKKQ 240

Query: 241 ANS-EAFLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPP 300
           ANS E FLNQRPPSRVE+VSGF WKRF+A+A QKPPTKAKRFAVIQAVNLRNR NPPLPP
Sbjct: 241 ANSDELFLNQRPPSRVESVSGFFWKRFIALAHQKPPTKAKRFAVIQAVNLRNRTNPPLPP 300

Query: 301 HSFGNLWWFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQM 360
           HSFGN+WWFATADAPIDEE +FPSLVAKIR AI EI+G YIKTLQ  EK+M+ KMKMGQM
Sbjct: 301 HSFGNIWWFATADAPIDEEPEFPSLVAKIRNAINEIDGHYIKTLQHAEKTMREKMKMGQM 360

Query: 361 VYSGELELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNL 420
           VYSGE+EL SFT WC+FPLYESDFGWGKP WVCSPGRPYKNVVLFVNTSDGEGIEAWVNL
Sbjct: 361 VYSGEVELISFTGWCNFPLYESDFGWGKPMWVCSPGRPYKNVVLFVNTSDGEGIEAWVNL 420

Query: 421 TETDMKDFETDSELLSFASLS 433
            E DMK FETD +LLSF S S
Sbjct: 421 EENDMKVFETDPQLLSFTSSS 441

BLAST of Cp4.1LG20g03280 vs. NCBI nr
Match: KAA0055309.1 (vinorine synthase-like [Cucumis melo var. makuwa] >TYJ99233.1 vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 701 bits (1810), Expect = 4.91e-252
Identity = 346/435 (79.54%), Postives = 383/435 (88.05%), Query Frame = 0

Query: 1   MEMNN-LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDD 60
           MEMNN  NVEIISTE IKPSSPTPS H+  KLSFLDQ AP SYTPLLFFY      GGDD
Sbjct: 1   MEMNNSFNVEIISTEIIKPSSPTPSTHQHHKLSFLDQFAPGSYTPLLFFYPG----GGDD 60

Query: 61  HRERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDV 120
           H +RCRKLKESLAETL+RFYLLAGTLVEAYLV+CND+GV FSEARVSG +SEVME P+DV
Sbjct: 61  HIDRCRKLKESLAETLSRFYLLAGTLVEAYLVECNDEGVPFSEARVSGHLSEVMENPNDV 120

Query: 121 VSLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAAC 180
           VSL RLLPF  D VLERECIL V+YNVFECGG V+A+CVTHK+VDGTS TMFTKAWA+ C
Sbjct: 121 VSLHRLLPFHPDVVLERECILGVQYNVFECGGAVIAVCVTHKVVDGTSTTMFTKAWASTC 180

Query: 181 RGDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEA- 240
           RGD+KYP+VPTFD+T+LFP MEIRG N+R   M KIVTRRFIFNKSNIAALK QANS A 
Sbjct: 181 RGDNKYPIVPTFDSTKLFPAMEIRGRNKRHPPMHKIVTRRFIFNKSNIAALKKQANSAAL 240

Query: 241 FLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNL 300
           FLNQRPPSRVE+VSGFLW RF+A++ +K PTKAKRFAVIQAVNLRNRMNPPLP HSFGN+
Sbjct: 241 FLNQRPPSRVESVSGFLWNRFIALSHKKTPTKAKRFAVIQAVNLRNRMNPPLPQHSFGNI 300

Query: 301 WWFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGEL 360
           WWFATAD PIDE+QDFPSLV KIREAIKEI+ +Y KTL+DTEKS++AKMKMG+ VYSGE+
Sbjct: 301 WWFATADVPIDEKQDFPSLVGKIREAIKEIDDEYTKTLEDTEKSLRAKMKMGERVYSGEV 360

Query: 361 ELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMK 420
           EL+SFTSWC+FP+YE+DFGWGKP WVCSPGRP+KNVVLFVNTSDGEGIEAWVNL + DM 
Sbjct: 361 ELFSFTSWCNFPVYETDFGWGKPTWVCSPGRPFKNVVLFVNTSDGEGIEAWVNLEKNDMA 420

Query: 421 DFETDSELLSFASLS 433
            FE DSELLSF S S
Sbjct: 421 LFENDSELLSFTSSS 431

BLAST of Cp4.1LG20g03280 vs. ExPASy TrEMBL
Match: A0A6J1E6N5 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111431282 PE=3 SV=1)

HSP 1 Score: 863 bits (2229), Expect = 0.0
Identity = 425/433 (98.15%), Postives = 433/433 (100.00%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH
Sbjct: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120
           RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGV+FSEARVSGRMSEVMEKPHDVV
Sbjct: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSEARVSGRMSEVMEKPHDVV 120

Query: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180
           SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR
Sbjct: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180

Query: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240
           GDDKYPVVPTFDATELFPPMEIRGGNRRQSRM+KIVTRRFIFNKSNIAALK+QANSEAFL
Sbjct: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMEKIVTRRFIFNKSNIAALKHQANSEAFL 240

Query: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW 300
           NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRM+PPLPP+SFGNLWW
Sbjct: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMDPPLPPNSFGNLWW 300

Query: 301 FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL 360
           FATADAPIDEEQDFPSLVAKIREAIKEI+GDYIKT+QDTEKSMKAKMKMGQMVYSGELEL
Sbjct: 301 FATADAPIDEEQDFPSLVAKIREAIKEIDGDYIKTIQDTEKSMKAKMKMGQMVYSGELEL 360

Query: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420
           YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF
Sbjct: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420

Query: 421 ETDSELLSFASLS 433
           ETD+ELLSFASLS
Sbjct: 421 ETDAELLSFASLS 433

BLAST of Cp4.1LG20g03280 vs. ExPASy TrEMBL
Match: A0A6J1KKZ8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111495142 PE=3 SV=1)

HSP 1 Score: 860 bits (2223), Expect = 3.44e-315
Identity = 425/433 (98.15%), Postives = 430/433 (99.31%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH
Sbjct: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 120
           RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGV+FSE RVS RMSEVMEKPHDVV
Sbjct: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVEFSETRVSARMSEVMEKPHDVV 120

Query: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180
           SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR
Sbjct: 121 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 180

Query: 181 GDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240
           GDDKYPVVPTF+ATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL
Sbjct: 181 GDDKYPVVPTFNATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFL 240

Query: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLWW 300
           NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRM+PPLPP+SFGNLWW
Sbjct: 241 NQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMDPPLPPNSFGNLWW 300

Query: 301 FATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELEL 360
           FATADAPIDEEQDFPSLVAKIREAIKEI+GDYIKTLQD EKSMKAKMKMGQMVYSGELEL
Sbjct: 301 FATADAPIDEEQDFPSLVAKIREAIKEIDGDYIKTLQDAEKSMKAKMKMGQMVYSGELEL 360

Query: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420
           YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF
Sbjct: 361 YSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDF 420

Query: 421 ETDSELLSFASLS 433
           ETDSELLSFASLS
Sbjct: 421 ETDSELLSFASLS 433

BLAST of Cp4.1LG20g03280 vs. ExPASy TrEMBL
Match: A0A5A7UPB9 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004440 PE=3 SV=1)

HSP 1 Score: 701 bits (1810), Expect = 2.38e-252
Identity = 346/435 (79.54%), Postives = 383/435 (88.05%), Query Frame = 0

Query: 1   MEMNN-LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDD 60
           MEMNN  NVEIISTE IKPSSPTPS H+  KLSFLDQ AP SYTPLLFFY      GGDD
Sbjct: 1   MEMNNSFNVEIISTEIIKPSSPTPSTHQHHKLSFLDQFAPGSYTPLLFFYPG----GGDD 60

Query: 61  HRERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDV 120
           H +RCRKLKESLAETL+RFYLLAGTLVEAYLV+CND+GV FSEARVSG +SEVME P+DV
Sbjct: 61  HIDRCRKLKESLAETLSRFYLLAGTLVEAYLVECNDEGVPFSEARVSGHLSEVMENPNDV 120

Query: 121 VSLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAAC 180
           VSL RLLPF  D VLERECIL V+YNVFECGG V+A+CVTHK+VDGTS TMFTKAWA+ C
Sbjct: 121 VSLHRLLPFHPDVVLERECILGVQYNVFECGGAVIAVCVTHKVVDGTSTTMFTKAWASTC 180

Query: 181 RGDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEA- 240
           RGD+KYP+VPTFD+T+LFP MEIRG N+R   M KIVTRRFIFNKSNIAALK QANS A 
Sbjct: 181 RGDNKYPIVPTFDSTKLFPAMEIRGRNKRHPPMHKIVTRRFIFNKSNIAALKKQANSAAL 240

Query: 241 FLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNL 300
           FLNQRPPSRVE+VSGFLW RF+A++ +K PTKAKRFAVIQAVNLRNRMNPPLP HSFGN+
Sbjct: 241 FLNQRPPSRVESVSGFLWNRFIALSHKKTPTKAKRFAVIQAVNLRNRMNPPLPQHSFGNI 300

Query: 301 WWFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGEL 360
           WWFATAD PIDE+QDFPSLV KIREAIKEI+ +Y KTL+DTEKS++AKMKMG+ VYSGE+
Sbjct: 301 WWFATADVPIDEKQDFPSLVGKIREAIKEIDDEYTKTLEDTEKSLRAKMKMGERVYSGEV 360

Query: 361 ELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMK 420
           EL+SFTSWC+FP+YE+DFGWGKP WVCSPGRP+KNVVLFVNTSDGEGIEAWVNL + DM 
Sbjct: 361 ELFSFTSWCNFPVYETDFGWGKPTWVCSPGRPFKNVVLFVNTSDGEGIEAWVNLEKNDMA 420

Query: 421 DFETDSELLSFASLS 433
            FE DSELLSF S S
Sbjct: 421 LFENDSELLSFTSSS 431

BLAST of Cp4.1LG20g03280 vs. ExPASy TrEMBL
Match: A0A0A0LUA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050210 PE=3 SV=1)

HSP 1 Score: 694 bits (1790), Expect = 2.64e-249
Identity = 339/435 (77.93%), Postives = 382/435 (87.82%), Query Frame = 0

Query: 1   MEMNN--LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGD 60
           MEMNN    ++IISTE IKPSSPTPS H+  KLSFLDQ AP SYTPLLFFY      GG 
Sbjct: 1   MEMNNNSFKIDIISTEIIKPSSPTPSTHQHHKLSFLDQFAPGSYTPLLFFYP-----GGG 60

Query: 61  DHRERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHD 120
           +HR+RCR+LKESLAETL+RFY LAGTLVEAYLV+CND+GV FSEARVSGR+SEVME P+D
Sbjct: 61  NHRDRCRRLKESLAETLSRFYPLAGTLVEAYLVECNDEGVAFSEARVSGRLSEVMENPND 120

Query: 121 VVSLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAA 180
           VV+  RLLPF  DAVLERECIL V+YNVFECGG V+ALC+THKIVDGTS TMFTKAWA+ 
Sbjct: 121 VVTYHRLLPFHPDAVLERECILGVQYNVFECGGAVIALCITHKIVDGTSTTMFTKAWAST 180

Query: 181 CRGDDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEA 240
           CRGD++YP+VP FDAT+LFP MEIRGGN+R  RM KIVTRRF+FNKSNIAALK QA+S A
Sbjct: 181 CRGDNEYPIVPNFDATDLFPAMEIRGGNKRHPRMQKIVTRRFVFNKSNIAALKKQASSAA 240

Query: 241 -FLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGN 300
            FLNQRPPSRVE+VSGFLWKRF+A+  +K PTKAKRFAVIQAVNLRNRMNPPLPP+SFGN
Sbjct: 241 LFLNQRPPSRVESVSGFLWKRFIALYHKKTPTKAKRFAVIQAVNLRNRMNPPLPPNSFGN 300

Query: 301 LWWFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGE 360
           +WWFATAD PIDEEQDFPSLV K+REAI+EI+ +Y KTLQDTEKS++AKMKMG+ VYSGE
Sbjct: 301 IWWFATADVPIDEEQDFPSLVGKVREAIREIDDEYTKTLQDTEKSLRAKMKMGERVYSGE 360

Query: 361 LELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDM 420
           +E+  FTSWC+FP+YE+DFGWGKP WVC+PGRPYKNVVLFVNTSDGEGIEAWVNL E+DM
Sbjct: 361 VEMACFTSWCNFPVYETDFGWGKPTWVCTPGRPYKNVVLFVNTSDGEGIEAWVNLEESDM 420

Query: 421 KDFETDSELLSFASL 432
             FE D ELLSF SL
Sbjct: 421 ALFENDCELLSFTSL 430

BLAST of Cp4.1LG20g03280 vs. ExPASy TrEMBL
Match: A0A6J1CAK2 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111008950 PE=3 SV=1)

HSP 1 Score: 694 bits (1790), Expect = 2.64e-249
Identity = 339/429 (79.02%), Postives = 382/429 (89.04%), Query Frame = 0

Query: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65
           L VEIISTESIKPSSPTP E RELKLS LDQVAP SYTPLLFFY A QL  GD H  RCR
Sbjct: 3   LKVEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGD-HGGRCR 62

Query: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRL 125
           KLKESLAETL RFYLLAGTLVEAYLV+CND+GV+FSEARV+ ++++VM+KP DV +L RL
Sbjct: 63  KLKESLAETLTRFYLLAGTLVEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRL 122

Query: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRGDDKY 185
           LPFD DAVL+R+CILAVRYN F+CGGVV+ALCVTHKIVDGTSAT FTKAWAAACRGD + 
Sbjct: 123 LPFDPDAVLDRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGDHEL 182

Query: 186 PVVPTFDATELFPPMEIRGGNRRQSRMDKIVTRRFIFNKSNIAALKNQANSEAFLNQRPP 245
            VVPTFDA ELFPPMEIRGGN+RQSRMDKIVT+RFIF+KSNIAALK +ANS+AFLNQRPP
Sbjct: 183 TVVPTFDAAELFPPMEIRGGNKRQSRMDKIVTKRFIFSKSNIAALKQEANSDAFLNQRPP 242

Query: 246 SRVETVSGFLWKRFMAIAQQKPPTKA-KRFAVIQAVNLRNRMNPPLPPHSFGNLWWFATA 305
           SRVETVS F+WKRFMA+A+ KP T A KRFA++ AVN+RNR++PPLPPHSFGNLWWFA A
Sbjct: 243 SRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWWFAVA 302

Query: 306 DAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQMVYSGELELYSFT 365
           +API+EE+D+P LV+KIR AI EI+G+Y+  LQD EK+M+AKMKMGQMVYSGE+ELYSFT
Sbjct: 303 EAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVYSGEVELYSFT 362

Query: 366 SWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTETDMKDFETDS 425
           SWCSFP+YESDFGWG+PAWVCSPGRPYKNVVL VNT DGEGIEAWVNL E DM  FE+D 
Sbjct: 363 SWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMPVFESDP 422

Query: 426 ELLSFASLS 433
           ELL+F S S
Sbjct: 423 ELLAFTSSS 430

BLAST of Cp4.1LG20g03280 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.8e-79
Identity = 170/440 (38.64%), Postives = 261/440 (59.32%), Query Frame = 0

Query: 6   LNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRERCR 65
           + V+++S + IKPSSPTP+  ++ KLS L+Q+ P+ + P++FFY+A   +      E+ +
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSA---NNSIKPTEQLQ 60

Query: 66  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRL 125
            LK+SL+ETL  FY LAG L     +DCND G DF EARV+  +S ++ +P    SL +L
Sbjct: 61  MLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSS-DSLQQL 120

Query: 126 LPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAA-ACRGDDK 185
           +P   D++  R  +L  + + FECG + + +C++HK+ D TS  +F K+WAA + RG  K
Sbjct: 121 IPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIK 180

Query: 186 YPVVPTFDATELFPPMEIRGGN-----RRQSRMDKIVTRRFIFNKSNIAALKNQANSEAF 245
               P FD  ++FPP      +       +  M++ +++RFIF+ S+I AL+ +A+S   
Sbjct: 181 TIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASS--- 240

Query: 246 LNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNL- 305
                P+RVE VS  +WK  M   +    T +K   +  +V+LR+R++PP   +S GNL 
Sbjct: 241 FEVNQPTRVEAVSALIWKSAMKATRTVSGT-SKPSILANSVSLRSRVSPPFTKNSIGNLV 300

Query: 306 -WWFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTL----QDTEKSMKAKMKMGQMV 365
            ++ A A+  I++ +   +LV+KIR+A +     +I  L      TE     + + G M+
Sbjct: 301 SYFAAKAEEGINQTK-LQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMI 360

Query: 366 YSGELELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLT 425
            SG+ + Y F+S C F LYE+DFGWGKP WV  P    KN+V  ++T +  GIEAWVNL 
Sbjct: 361 ASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNLN 420

Query: 426 ETDMKDFETDSELLSFASLS 434
           E +M  FE D ELL FASL+
Sbjct: 421 EQEMNLFEQDRELLQFASLN 431

BLAST of Cp4.1LG20g03280 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 286.6 bits (732), Expect = 3.4e-77
Identity = 166/436 (38.07%), Postives = 251/436 (57.57%), Query Frame = 0

Query: 6   LNVEIISTESIKPS----SPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHR 65
           L + + S E +KPS    +  P  H    LSFLDQ+AP  + P LFFY  ++ +  D  +
Sbjct: 3   LEITVTSQELVKPSPRNLNHPPCHHH---LSFLDQLAPPIFMPFLFFY-HNKTNLSD--K 62

Query: 66  ERCRKLKESLAETLNRFYLLAGTLVEA-YLVDCNDDGVDFSEARVSGRMSEVMEKPHDVV 125
           ER   +K SL+E LN +Y LAG +  +  +V CND GV F EA+    MS+++E P+   
Sbjct: 63  ERSDHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNP-N 122

Query: 126 SLLRLLPFDHDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACR 185
            L +L PF+   V   +  L V+   FECGG+ + + ++HK+ D  S  +F  +WAA  R
Sbjct: 123 ELNKLHPFEFHEV--SDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFAR 182

Query: 186 GDDKYPVVPTFDATELFPPMEIRGGNRRQS-RMDKIVTRRFIFNKSNIAALKNQANSEAF 245
           G     + P+FD  ++FPP +I   N       + IVTRRF+F +S++ +L+ + +    
Sbjct: 183 GQTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFSGNKK 242

Query: 246 LNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPHSFGNLW 305
           +     +RVE +S F+W RFMA       T  K + +I  VNLR + +P +P + FGN+ 
Sbjct: 243 IR---ATRVEVLSVFIWSRFMASTNHDDKT-GKIYTLIHPVNLRRQADPDIPDNMFGNIM 302

Query: 306 WFATADAPI---DEEQDFPSLVAKIREAIKEINGDYIKTLQ-DTEKSMKAKMKMGQMVYS 365
            F+     +   + +++  SLV ++RE I++I+  Y+K LQ D    ++   K      +
Sbjct: 303 RFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFVN 362

Query: 366 GELELYSFTSWCSFPLYESDFGWGKPAWVCSPGRPYKNVVLFVNTSDGEGIEAWVNLTET 425
           GE+  +SFTS C FP+YE+DFGWGKP WV S    YKN+V F++T +G+GIEAW+NL + 
Sbjct: 363 GEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQN 422

Query: 426 DMKDFETDSELLSFAS 432
           DM  FE D ELL + S
Sbjct: 423 DMSRFEADEELLRYVS 425

BLAST of Cp4.1LG20g03280 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-67
Identity = 172/444 (38.74%), Postives = 250/444 (56.31%), Query Frame = 0

Query: 7   NVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTAD-QLHGGDDHRERCR 66
           NVEI+S E +KPSSPTP + R L LS LD ++   YT  L FY AD Q   G    E   
Sbjct: 4   NVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSL 63

Query: 67  KLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSGRMSEVMEKPHDVVSLLRL 126
           KLK+SL++TL  FY LAG ++ ++ V+CND+G  F EARV   +SE ++ P    SL  L
Sbjct: 64  KLKKSLSKTLPIFYPLAGRIIGSF-VECNDEGAVFIEARVDHLLSEFLKCPVP-ESLELL 123

Query: 127 LPFDHDAVLEREC----ILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWAAACRG 186
           +P +      RE     +L ++ N F CGG+V+ +CV+HKI D TS  MF + WA + RG
Sbjct: 124 IPVE---AKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 183

Query: 187 DDKYPVVPTFDATELFPPMEIRGGNRRQSRMDKI-----VTRRFIFNKSNIAALKNQANS 246
                ++P+F A+E+FP       ++   R +++     VT+RF+F+ S I  L+ +A+ 
Sbjct: 184 LG-ITLIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKASR 243

Query: 247 EAFLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQ-AVNLRNRMNPPLPPHSF 306
               N   P+RVE V+   W+    +++    T   R +V+Q  VNLR +++  L  ++ 
Sbjct: 244 NLVKN---PTRVEAVTALFWRCVTKVSRLSSLT--PRTSVLQILVNLRGKVD-SLCENTI 303

Query: 307 GN---LWWFATADAPIDEEQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAKMKMGQM 366
           GN   L      +A I+  QD   +V +IR A KEI     K +  +   +   ++    
Sbjct: 304 GNMLSLMILKNEEAAIERIQD---VVDEIRRA-KEIFSLNCKEMSKSSSRIFELLEEIGK 363

Query: 367 VY--SGELELYSFTSWCSFPLYESDFGWGKPAWVCSPGRP-YKNVVLFVNTSDGEGIEAW 426
           VY    E++L+   SWC   LY++DFGWGKP WV   G   +KN++L ++T DGEGIEAW
Sbjct: 364 VYGRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAW 423

Query: 427 VNLTETDMKDFETDSELLSFASLS 434
           + LTE  M  FE D ELL  ASL+
Sbjct: 424 ITLTEEQMSLFECDQELLESASLN 431

BLAST of Cp4.1LG20g03280 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 6.6e-65
Identity = 159/453 (35.10%), Postives = 246/453 (54.30%), Query Frame = 0

Query: 3   MNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDHRE 62
           M  + VE I+ E IKPSS TP++ + L+LS  D + P  YT    FYT D L   +   +
Sbjct: 1   METMKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDLISPE---Q 60

Query: 63  RCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSG-RMSEVMEKPHDVVS 122
              KLK SLAETL +FY LAG  ++   +DCND+G  F +ARV+   +S+ +  P D  +
Sbjct: 61  SSHKLKTSLAETLTKFYPLAGR-IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSP-DFKT 120

Query: 123 LLRLLPFD-----HDAVLERECILAVRYNVFECGGVVVALCVTHKIVDGTSATMFTKAWA 182
           L +LLP D     ++A      +L V+   F CGG+ + LC+THKI D TS + F ++WA
Sbjct: 121 LQQLLPLDVIDDPYEAATTWP-LLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWA 180

Query: 183 AACRGDDKYPVV-PTFDATELFPPMEIRGGNRRQSRMDKI--VTRRFIFNKSNIAALKNQ 242
           A  RG+    V  P F A   +PP             +KI  +T+RF+F+ S +  L+ +
Sbjct: 181 AMARGEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTK 240

Query: 243 ANSEAFLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNPPLPPH 302
           A SE F+ +  P RVE+V+  LWK F+A A            +IQ  NLR ++   L   
Sbjct: 241 AASEDFVGR--PKRVESVTALLWKAFVAAASSN-TNACDMKVLIQPSNLRPKIPSLLAES 300

Query: 303 SFGNLWWFATADAPIDEEQDFPSLVAKIREAIKEI--NGDYIKTLQDTEKSMKAKM---K 362
             GN+  F++    I ++++      KI +A++++   GD ++ +   E    + M   K
Sbjct: 301 LIGNI-MFSSVVLGISQQEEI-----KIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSK 360

Query: 363 MGQMVYSGELEL-------YSFTSWCSFPLYESDFGWGKPAWVCSPGRP-YKNVVLFVNT 422
           +  ++ +   +L       Y+ +SWC  PLYE+ FGWG P W+     P  +NV + +++
Sbjct: 361 LANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDS 420

Query: 423 SDGEGIEAWVNLTETDMKDFETDSELLSFASLS 434
            DG+GIEA+V L E +M   E ++ELL+FAS++
Sbjct: 421 KDGQGIEAFVTLPEENMLSLEQNTELLAFASVN 438

BLAST of Cp4.1LG20g03280 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 232.6 bits (592), Expect = 5.8e-61
Identity = 152/455 (33.41%), Postives = 236/455 (51.87%), Query Frame = 0

Query: 1   MEMNNLNVEIISTESIKPSSPTPSEHRELKLSFLDQVAPSSYTPLLFFYTADQLHGGDDH 60
           ME   + VE IS E IKPSSPTP+  + L+LS  D + P  YT    FYT + L      
Sbjct: 1   METMTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDL---ISQ 60

Query: 61  RERCRKLKESLAETLNRFYLLAGTLVEAYLVDCNDDGVDFSEARVSG-RMSEVMEKPHDV 120
                KLK SL+ETL +FY LAG  +    VDC D+G  F +ARV+   ++E ++ P D 
Sbjct: 61  EHTSHKLKTSLSETLTKFYPLAGR-ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCP-DF 120

Query: 121 VSLLRLLPFDHDAVLERECI-------LAVRYNVFECGGVVVALCVTHKIVDGTSATMFT 180
            +L +LLP D   V++   +       L V+   F CGG+ + +C+THKI D  S + F 
Sbjct: 121 DALQQLLPLD---VVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFI 180

Query: 181 KAWAAACRGDDKYPVV--PTFDATELFPPME--IRGGNRRQSRMDKIVTRRFIFNKSNIA 240
           ++WAA  RG++    +  P F     +PP     +     Q+     +T+RF+F  S + 
Sbjct: 181 RSWAATARGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVE 240

Query: 241 ALKNQANSEAFLNQRPPSRVETVSGFLWKRFMAIAQQKPPTKAKRFAVIQAVNLRNRMNP 300
            L+ +A SE  ++Q  P+RVE+V+  +WK F+A ++    T      ++Q  NLR+++  
Sbjct: 241 DLRTKAASEETVDQ--PTRVESVTALIWKCFVASSK---TTTCDHKVLVQLANLRSKIPS 300

Query: 301 PLPPHSFGNLWWFATADAPIDE--EQDFPSLVAKIREAIKEINGDYIKTLQDTEKSMKAK 360
            L   S GNL  F++    I    E      V  +R+  +E+    +     ++ S    
Sbjct: 301 LLQESSIGNL-MFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIG 360

Query: 361 MKMGQMVYSGELEL-------YSFTSWCSFPLYESDFGWGKPAWVCSPGRP-YKNVVLFV 420
            K+  ++ +    L       Y+ +SWC  PLYE+ FGW  P WV     P   N+ + +
Sbjct: 361 SKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLI 420

Query: 421 NTSDGEGIEAWVNLTETDMKDFETDSELLSFASLS 434
           ++ DG+GIEA+V L E +M  FE + ELL+FA+++
Sbjct: 421 DSKDGQGIEAFVTLPEENMSSFEQNPELLAFATMN 441