Cp4.1LG20g03220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g03220 vs. ExPASy Swiss-Prot
Match: Q9STM6 (GDSL esterase/lipase At3g48460 OS=Arabidopsis thaliana OX=3702 GN=At3g48460 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.9e-131
Identity = 225/376 (59.84%), Postives = 280/376 (74.47%), Query Frame = 0

Query: 5   LTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 64
           LT+  ++ ILL   +  + T    H R FNKIYAFGDSFTDTGN+RS  GPAGFGH+S+ 
Sbjct: 9   LTTAISVAILLFSTISTAATIPNIH-RPFNKIYAFGDSFTDTGNSRSGEGPAGFGHLSSP 68

Query: 65  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDS------FHGVNFAVAG 124
           PYG TFF   TNRYSDGRL IDFVA+S++LPFLPPY  LK  ++       HGVNFAV+G
Sbjct: 69  PYGMTFFRRPTNRYSDGRLTIDFVAESMNLPFLPPYLSLKTTNANGTATDTHGVNFAVSG 128

Query: 125 STAINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVG 184
           ST I H FFV+NNLSLD+TPQSI+T+L WF K+LET G        Q  + F D+LFW+G
Sbjct: 129 STVIKHAFFVKNNLSLDMTPQSIETELAWFEKYLETLG------TNQKVSLFKDSLFWIG 188

Query: 185 EIGVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSL 244
           EIGVNDYAY +GS+++ DTIR+L +++ T  L++LL KG KY++VQG P +GCL LAMSL
Sbjct: 189 EIGVNDYAYTLGSTVSSDTIRELSISTFTRFLETLLNKGVKYMLVQGHPATGCLTLAMSL 248

Query: 245 APVTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSK 304
           A   DRD +GCV+S NNQ+Y HN+ALQ+ L+ LR ++P A I+YADYWNAYR V+K+PSK
Sbjct: 249 AAEDDRDSLGCVQSANNQSYTHNLALQSKLKQLRIKYPSATIVYADYWNAYRAVIKHPSK 308

Query: 305 YGFRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFI 364
           YG  E+FKACCG+GEPYNF +F  CG  + + CK P +YINWDGVHLTEAMYK + DMF+
Sbjct: 309 YGITEKFKACCGIGEPYNFQVFQTCGTDAATVCKDPNQYINWDGVHLTEAMYKVMADMFL 368

Query: 365 KGGLTHPALSNLLDMK 375
            G  T P  S+LL  K
Sbjct: 369 DGTFTRPRFSDLLIKK 377

BLAST of Cp4.1LG20g03220 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.4e-62
Identity = 135/369 (36.59%), Postives = 199/369 (53.93%), Query Frame = 0

Query: 5   LTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 64
           + S  ++ I L   +  +  ++ET    F  I +FGDS  DTGN   +S        +  
Sbjct: 1   MASLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFP 60

Query: 65  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAINH 124
           PYG TFFHH T R  DGR+++DF+A+ + LP++PPY   K  +   GVNFAVAG+TA+  
Sbjct: 61  PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKS 120

Query: 125 GFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGVND 184
            F  +  +    T  S+  QL  F K L         + + C+    +AL  +GEIG ND
Sbjct: 121 SFLKKRGIQ-PHTNVSLGVQLKSFKKSLPNL----CGSPSDCRDMIGNALILMGEIGGND 180

Query: 185 YAYAVGSSITDDTIRKL---GVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPV 244
           Y +   +      + +L    +AS++  +  L+  G K  +V G  P GC  + ++L   
Sbjct: 181 YNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKT 240

Query: 245 TDRDD----IGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPS 304
           +++D+     GC+K LN     H+  L+  L  LR+ +P   IIYADY+N+   + K P+
Sbjct: 241 SNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPA 300

Query: 305 KYGFRER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDM 364
           K+GF ER F ACCG+G PYNF+    CG   V SCK P +Y+ WDGVH+TEA YK + D 
Sbjct: 301 KFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADG 360

Query: 365 FIKGGLTHP 366
            + G   +P
Sbjct: 361 ILNGPYANP 364

BLAST of Cp4.1LG20g03220 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 141/370 (38.11%), Postives = 202/370 (54.59%), Query Frame = 0

Query: 5   LTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 64
           + S    F+L+  +      ++E+  R +  I +FGDS  DTGN   +S        +  
Sbjct: 7   MASLIVSFLLILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFL 66

Query: 65  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFH-GVNFAVAGSTAIN 124
           PYG +FFH  + RYSDGRLVIDF+A+ L LP++PPY +   N SF+ G+NFAV G+TA++
Sbjct: 67  PYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPY-FGSQNVSFNQGINFAVYGATALD 126

Query: 125 HGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGVN 184
             F V+  +  D T  S+  QL  F + L    C  A +   C+    D+L  +GEIG N
Sbjct: 127 RAFLVKQGIKSDFTNISLSVQLNTFKQILPNL-C--ASSTRDCREMLGDSLILMGEIGGN 186

Query: 185 DYAYAV--GSSITD-DTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSL-- 244
           DY Y    G SI +   +  L + +++  +  L+  G K  +V G  P GC    ++L  
Sbjct: 187 DYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQ 246

Query: 245 -APVTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPS 304
            A V      GC+  LN     HN  L+  L+ L++ +P   IIYADY+N+   + + P+
Sbjct: 247 TATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPA 306

Query: 305 KYGFRER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDM 364
           KYGF+ R   ACCGVG  YNF +   CG   VS C++P EY+NWDG HLTEA Y+ +   
Sbjct: 307 KYGFKNRPLAACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQG 366

Query: 365 FIKGGLTHPA 367
            + G  T PA
Sbjct: 367 LLNGRYTTPA 372

BLAST of Cp4.1LG20g03220 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.1e-60
Identity = 135/337 (40.06%), Postives = 185/337 (54.90%), Query Frame = 0

Query: 33  FNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSL 92
           F+ IY  GDSF+DTGN   +         ++ PYG TF    T R SDGRL+IDF+A +L
Sbjct: 27  FDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIATAL 86

Query: 93  SLPFLPPYKYLKGNDSF-HGVNFAVAGSTAINHGFFVRNNLSLDITPQSIQTQLLWFNKF 152
           +LP L P  YL+ N SF HGVNFAVAG+TA++  F     + +      +  QL WF  +
Sbjct: 87  NLPLLNP--YLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTY 146

Query: 153 LETQGCRGAETKTQCKAAFDDALFWVGEIGVNDYAYAVGSSITDDTIR---KLGVASVTG 212
           L +       T  +C     +ALF +G IG ND  YA  +  T + IR        +V  
Sbjct: 147 LGSI----CSTPKECSNKLKNALFILGNIGNNDVNYAFPNR-TIEEIRAYVPFITEAVAN 206

Query: 213 VLQSLLKKGAKYVVVQGLPPSGCLALAMSLA---PVTDRDDIGCVKSLNNQTYVHNIALQ 272
             + +++ G   V+V G+ P GC+A  ++     P  D+DD+GC+ SLNN +   N   Q
Sbjct: 207 ATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQ 266

Query: 273 ASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGFRER--FKACCGVGEPYNFDMFTVC 332
            +L SL  +FPQAVIIYADY+NA+R + +N    G       K CCG+G PYN+D    C
Sbjct: 267 RALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDREC 326

Query: 333 GMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 361
           G   V  C +P +YI WDG H T+A Y+ V +  I G
Sbjct: 327 GSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPG 356

BLAST of Cp4.1LG20g03220 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.9e-60
Identity = 135/364 (37.09%), Postives = 199/364 (54.67%), Query Frame = 0

Query: 11  IFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNSPYGSTF 70
           +FI L  FV+ +  ++ET  R F  I +FGDS  DTGN   +S P    H++  PYG  F
Sbjct: 14  VFIFLSTFVVTN-VSSETKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENF 73

Query: 71  FHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFH-GVNFAVAGSTAINHGFFVR 130
           FHH T R+S+GRL+IDF+A+ L LP +PP+ Y   N +F  GVNFAV G+TA+   F   
Sbjct: 74  FHHPTGRFSNGRLIIDFIAEFLGLPLVPPF-YGSHNANFEKGVNFAVGGATALERSFLED 133

Query: 131 NNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGVNDYAYA- 190
             +    T  S+  QL  F + L +       + + C+   ++AL  +GEIG NDY YA 
Sbjct: 134 RGIHFPYTNVSLGVQLNSFKESLPSI----CGSPSDCRDMIENALILMGEIGGNDYNYAF 193

Query: 191 -VGSSITD-DTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPVTDRDD 250
            V   I +   +  L + +++  +  L+  G +  +V G  P GC  L ++    ++ ++
Sbjct: 194 FVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEE 253

Query: 251 ----IGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGFR 310
                GC+K LN     H   L+A L  L++ +P   IIYADY+NA   + + P+K+GF 
Sbjct: 254 YDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFGFM 313

Query: 311 ER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKGG 366
            R   ACCG G PYN+ +   CG   V SC  P +Y+ WDGVH+TEA Y+ + +  + G 
Sbjct: 314 NRPLSACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMAEGILNGP 371

BLAST of Cp4.1LG20g03220 vs. NCBI nr
Match: XP_023520155.1 (GDSL esterase/lipase At3g48460 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 769 bits (1985), Expect = 1.38e-280
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI
Sbjct: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP
Sbjct: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 361 GLTHPALSNLLDMKHH 376

BLAST of Cp4.1LG20g03220 vs. NCBI nr
Match: XP_022923808.1 (GDSL esterase/lipase At3g48460 [Cucurbita moschata])

HSP 1 Score: 765 bits (1976), Expect = 3.25e-279
Identity = 373/376 (99.20%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI
Sbjct: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLALAMSLAP
Sbjct: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAP 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+SLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 241 VTDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCK+PPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKG 360

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 361 GLTHPALSNLLDMKHH 376

BLAST of Cp4.1LG20g03220 vs. NCBI nr
Match: KAG6584128.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 763 bits (1970), Expect = 2.67e-278
Identity = 372/376 (98.94%), Postives = 375/376 (99.73%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQ CRGAETKTQCKAAFDDALFWVGEI
Sbjct: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQACRGAETKTQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLALAMSLAP
Sbjct: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAP 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+SLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 241 VTDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCK+PPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKG 360

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 361 GLTHPALSNLLDMKHH 376

BLAST of Cp4.1LG20g03220 vs. NCBI nr
Match: XP_023001531.1 (GDSL esterase/lipase At3g48460-like [Cucurbita maxima])

HSP 1 Score: 753 bits (1943), Expect = 3.23e-274
Identity = 369/376 (98.14%), Postives = 372/376 (98.94%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVA  SCTAIFILLC FVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 1   MMVA--SCTAIFILLCSFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFN FLETQGCRGAETKTQCKAAFDDALFWVGEI
Sbjct: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNMFLETQGCRGAETKTQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLALAMSLAP
Sbjct: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAP 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+SLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 241 VTDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCK+PPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKG 360

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 361 GLTHPALSNLLDMKHH 374

BLAST of Cp4.1LG20g03220 vs. NCBI nr
Match: KAG7019716.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 763 bits (1970), Expect = 5.11e-274
Identity = 372/376 (98.94%), Postives = 375/376 (99.73%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 268 MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 327

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 328 VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 387

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQ CRGAETKTQCKAAFDDALFWVGEI
Sbjct: 388 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQACRGAETKTQCKAAFDDALFWVGEI 447

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLALAMSLAP
Sbjct: 448 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAP 507

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+SLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 508 VTDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 567

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCK+PPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 568 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKG 627

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 628 GLTHPALSNLLDMKHH 643

BLAST of Cp4.1LG20g03220 vs. ExPASy TrEMBL
Match: A0A6J1EAM5 (GDSL esterase/lipase At3g48460 OS=Cucurbita moschata OX=3662 GN=LOC111431411 PE=3 SV=1)

HSP 1 Score: 765 bits (1976), Expect = 1.57e-279
Identity = 373/376 (99.20%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI
Sbjct: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLALAMSLAP
Sbjct: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAP 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+SLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 241 VTDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCK+PPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKG 360

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 361 GLTHPALSNLLDMKHH 376

BLAST of Cp4.1LG20g03220 vs. ExPASy TrEMBL
Match: A0A6J1KIW4 (GDSL esterase/lipase At3g48460-like OS=Cucurbita maxima OX=3661 GN=LOC111495644 PE=3 SV=1)

HSP 1 Score: 753 bits (1943), Expect = 1.56e-274
Identity = 369/376 (98.14%), Postives = 372/376 (98.94%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVA  SCTAIFILLC FVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH
Sbjct: 1   MMVA--SCTAIFILLCSFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINHGFFVRNNLSLDITPQSIQTQLLWFN FLETQGCRGAETKTQCKAAFDDALFWVGEI
Sbjct: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNMFLETQGCRGAETKTQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLALAMSLAP
Sbjct: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAP 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+SLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG
Sbjct: 241 VTDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNFDMFTVCGMPSVSSCK+PPEYINWDGVHLTEAMYKAVHDMFIKG
Sbjct: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKG 360

Query: 361 GLTHPALSNLLDMKHH 376
           GLTHPALSNLLDMKHH
Sbjct: 361 GLTHPALSNLLDMKHH 374

BLAST of Cp4.1LG20g03220 vs. ExPASy TrEMBL
Match: A0A6J1C9C3 (GDSL esterase/lipase At3g48460 OS=Momordica charantia OX=3673 GN=LOC111008661 PE=3 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 2.00e-244
Identity = 327/374 (87.43%), Postives = 348/374 (93.05%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           M+ +LT  TA+++LLC F  ASPTATETHPRLFNKIYAFGDSFTDTGNTRS+SGP GFGH
Sbjct: 2   MVPSLTYYTAVYVLLCAFASASPTATETHPRLFNKIYAFGDSFTDTGNTRSMSGPTGFGH 61

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VS+ PYGSTFFHH TNRYSDGRLVIDFVA++LSLPFLPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 62  VSSPPYGSTFFHHPTNRYSDGRLVIDFVAETLSLPFLPPYKYLKGNDSFHGVNFAVAGST 121

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINH FFVRNNLSLDITPQSIQTQLLWFN+FLETQGCRGAET+ QCKAA DDALFWVGEI
Sbjct: 122 AINHDFFVRNNLSLDITPQSIQTQLLWFNEFLETQGCRGAETEKQCKAALDDALFWVGEI 181

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAY +GS I DDTIRKLGVASVTG LQSLLKKGAKYVVVQGLPPSGCLALAMSLAP
Sbjct: 182 GVNDYAYDIGSPIPDDTIRKLGVASVTGFLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 241

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           VTDRDDIGCV+S++NQTYVHN+ALQASLQSLR QFPQAVIIYADYWNAYR V+KNPS YG
Sbjct: 242 VTDRDDIGCVRSVSNQTYVHNVALQASLQSLRGQFPQAVIIYADYWNAYRTVIKNPSNYG 301

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           F+ERFKACCGVGEPYNFD+FTVCGM SVSSCK+P EYINWDGVHLTEAMYK VHDMFI+G
Sbjct: 302 FKERFKACCGVGEPYNFDVFTVCGMSSVSSCKNPSEYINWDGVHLTEAMYKIVHDMFIEG 361

Query: 361 GLTHPALSNLLDMK 374
           GLT P  S LLDMK
Sbjct: 362 GLTRPPFSYLLDMK 375

BLAST of Cp4.1LG20g03220 vs. ExPASy TrEMBL
Match: A0A0A0LWI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050100 PE=3 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 5.45e-244
Identity = 327/376 (86.97%), Postives = 347/376 (92.29%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMV LT C+AIFILL  F  ASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGP+GFGH
Sbjct: 31  MMVTLTYCSAIFILLFAFASASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPSGFGH 90

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VSN PYGSTFFHH TNRYSDGRLVIDFVAQSLSLP LPPY+YLKGNDSFHGVNFAVAGST
Sbjct: 91  VSNPPYGSTFFHHPTNRYSDGRLVIDFVAQSLSLPLLPPYRYLKGNDSFHGVNFAVAGST 150

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINH F+VRNNLS+DITPQSIQTQLLWFNKFLETQGCRG ETK QC+AAFDDAL WVGEI
Sbjct: 151 AINHEFYVRNNLSIDITPQSIQTQLLWFNKFLETQGCRGEETKAQCEAAFDDALLWVGEI 210

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAY+ GS I+ DTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCLAL+MSLA 
Sbjct: 211 GVNDYAYSFGSPISPDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALSMSLAS 270

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           V DRDDIGCV+SLNNQTYVH++ALQASLQSLR QFP+AVIIYADYWNAYR V+KNP+KYG
Sbjct: 271 VDDRDDIGCVRSLNNQTYVHSMALQASLQSLRRQFPEAVIIYADYWNAYRTVIKNPNKYG 330

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           F ERFKACCGVGEPYNF++FTVCGM SVSSCK+P EYINWDGVHLTEAMYK VHDM I+G
Sbjct: 331 FSERFKACCGVGEPYNFELFTVCGMSSVSSCKTPSEYINWDGVHLTEAMYKVVHDMLIEG 390

Query: 361 GLTHPALSNLLDMKHH 376
           G THP  SNLLDMK H
Sbjct: 391 GFTHPPFSNLLDMKRH 406

BLAST of Cp4.1LG20g03220 vs. ExPASy TrEMBL
Match: A0A1S3AYE5 (GDSL esterase/lipase At3g48460 OS=Cucumis melo OX=3656 GN=LOC103484109 PE=3 SV=1)

HSP 1 Score: 674 bits (1738), Expect = 3.18e-243
Identity = 328/376 (87.23%), Postives = 345/376 (91.76%), Query Frame = 0

Query: 1   MMVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGH 60
           MMVALT C+AIFILL  F  ASPTA ETHPRLFNKIYAFGDSFTDTGNTRS SGPAGFGH
Sbjct: 1   MMVALTYCSAIFILLFAFASASPTAIETHPRLFNKIYAFGDSFTDTGNTRSASGPAGFGH 60

Query: 61  VSNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGST 120
           VS+ PYGSTFFHH TNRYSDGRLVIDFVAQSLSLP LPPYKYLKGNDSFHGVNFAVAGST
Sbjct: 61  VSDPPYGSTFFHHPTNRYSDGRLVIDFVAQSLSLPLLPPYKYLKGNDSFHGVNFAVAGST 120

Query: 121 AINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEI 180
           AINH F+VRNNLS+D TPQSIQTQLLWFNKFLETQGCRG ETK QCKAAFDDALFWVGEI
Sbjct: 121 AINHEFYVRNNLSIDNTPQSIQTQLLWFNKFLETQGCRGEETKAQCKAAFDDALFWVGEI 180

Query: 181 GVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAP 240
           GVNDYAY+ GS I+ DTIRKL VASVTGVLQSLLKKGAKY+VVQGLPPSGCLAL+MSLA 
Sbjct: 181 GVNDYAYSFGSPISADTIRKLAVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALSMSLAS 240

Query: 241 VTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYG 300
           V DRDDIGCV+SLNNQTYVH++ALQASLQSLR QFP+AVIIYADYWNAYR V+KNPSKYG
Sbjct: 241 VDDRDDIGCVRSLNNQTYVHSMALQASLQSLRRQFPEAVIIYADYWNAYRTVIKNPSKYG 300

Query: 301 FRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKG 360
           FRERFKACCGVGEPYNF++FTVCGM SVSSCK+P EYINWDGVHLTEAMYK VHDM I+G
Sbjct: 301 FRERFKACCGVGEPYNFELFTVCGMSSVSSCKTPSEYINWDGVHLTEAMYKVVHDMLIEG 360

Query: 361 GLTHPALSNLLDMKHH 376
           G THP  S LLDMK H
Sbjct: 361 GFTHPPFSYLLDMKRH 376

BLAST of Cp4.1LG20g03220 vs. TAIR 10
Match: AT3G48460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 4.2e-132
Identity = 225/376 (59.84%), Postives = 280/376 (74.47%), Query Frame = 0

Query: 5   LTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 64
           LT+  ++ ILL   +  + T    H R FNKIYAFGDSFTDTGN+RS  GPAGFGH+S+ 
Sbjct: 9   LTTAISVAILLFSTISTAATIPNIH-RPFNKIYAFGDSFTDTGNSRSGEGPAGFGHLSSP 68

Query: 65  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDS------FHGVNFAVAG 124
           PYG TFF   TNRYSDGRL IDFVA+S++LPFLPPY  LK  ++       HGVNFAV+G
Sbjct: 69  PYGMTFFRRPTNRYSDGRLTIDFVAESMNLPFLPPYLSLKTTNANGTATDTHGVNFAVSG 128

Query: 125 STAINHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVG 184
           ST I H FFV+NNLSLD+TPQSI+T+L WF K+LET G        Q  + F D+LFW+G
Sbjct: 129 STVIKHAFFVKNNLSLDMTPQSIETELAWFEKYLETLG------TNQKVSLFKDSLFWIG 188

Query: 185 EIGVNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSL 244
           EIGVNDYAY +GS+++ DTIR+L +++ T  L++LL KG KY++VQG P +GCL LAMSL
Sbjct: 189 EIGVNDYAYTLGSTVSSDTIRELSISTFTRFLETLLNKGVKYMLVQGHPATGCLTLAMSL 248

Query: 245 APVTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSK 304
           A   DRD +GCV+S NNQ+Y HN+ALQ+ L+ LR ++P A I+YADYWNAYR V+K+PSK
Sbjct: 249 AAEDDRDSLGCVQSANNQSYTHNLALQSKLKQLRIKYPSATIVYADYWNAYRAVIKHPSK 308

Query: 305 YGFRERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFI 364
           YG  E+FKACCG+GEPYNF +F  CG  + + CK P +YINWDGVHLTEAMYK + DMF+
Sbjct: 309 YGITEKFKACCGIGEPYNFQVFQTCGTDAATVCKDPNQYINWDGVHLTEAMYKVMADMFL 368

Query: 365 KGGLTHPALSNLLDMK 375
            G  T P  S+LL  K
Sbjct: 369 DGTFTRPRFSDLLIKK 377

BLAST of Cp4.1LG20g03220 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.4e-63
Identity = 135/369 (36.59%), Postives = 199/369 (53.93%), Query Frame = 0

Query: 5   LTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 64
           + S  ++ I L   +  +  ++ET    F  I +FGDS  DTGN   +S        +  
Sbjct: 1   MASLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFP 60

Query: 65  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAINH 124
           PYG TFFHH T R  DGR+++DF+A+ + LP++PPY   K  +   GVNFAVAG+TA+  
Sbjct: 61  PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKS 120

Query: 125 GFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGVND 184
            F  +  +    T  S+  QL  F K L         + + C+    +AL  +GEIG ND
Sbjct: 121 SFLKKRGIQ-PHTNVSLGVQLKSFKKSLPNL----CGSPSDCRDMIGNALILMGEIGGND 180

Query: 185 YAYAVGSSITDDTIRKL---GVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPV 244
           Y +   +      + +L    +AS++  +  L+  G K  +V G  P GC  + ++L   
Sbjct: 181 YNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKT 240

Query: 245 TDRDD----IGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPS 304
           +++D+     GC+K LN     H+  L+  L  LR+ +P   IIYADY+N+   + K P+
Sbjct: 241 SNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPA 300

Query: 305 KYGFRER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDM 364
           K+GF ER F ACCG+G PYNF+    CG   V SCK P +Y+ WDGVH+TEA YK + D 
Sbjct: 301 KFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADG 360

Query: 365 FIKGGLTHP 366
            + G   +P
Sbjct: 361 ILNGPYANP 364

BLAST of Cp4.1LG20g03220 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 9.2e-63
Identity = 141/370 (38.11%), Postives = 202/370 (54.59%), Query Frame = 0

Query: 5   LTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 64
           + S    F+L+  +      ++E+  R +  I +FGDS  DTGN   +S        +  
Sbjct: 7   MASLIVSFLLILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFL 66

Query: 65  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFH-GVNFAVAGSTAIN 124
           PYG +FFH  + RYSDGRLVIDF+A+ L LP++PPY +   N SF+ G+NFAV G+TA++
Sbjct: 67  PYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPY-FGSQNVSFNQGINFAVYGATALD 126

Query: 125 HGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGVN 184
             F V+  +  D T  S+  QL  F + L    C  A +   C+    D+L  +GEIG N
Sbjct: 127 RAFLVKQGIKSDFTNISLSVQLNTFKQILPNL-C--ASSTRDCREMLGDSLILMGEIGGN 186

Query: 185 DYAYAV--GSSITD-DTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSL-- 244
           DY Y    G SI +   +  L + +++  +  L+  G K  +V G  P GC    ++L  
Sbjct: 187 DYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQ 246

Query: 245 -APVTDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPS 304
            A V      GC+  LN     HN  L+  L+ L++ +P   IIYADY+N+   + + P+
Sbjct: 247 TATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPA 306

Query: 305 KYGFRER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDM 364
           KYGF+ R   ACCGVG  YNF +   CG   VS C++P EY+NWDG HLTEA Y+ +   
Sbjct: 307 KYGFKNRPLAACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQG 366

Query: 365 FIKGGLTHPA 367
            + G  T PA
Sbjct: 367 LLNGRYTTPA 372

BLAST of Cp4.1LG20g03220 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-61
Identity = 135/364 (37.09%), Postives = 199/364 (54.67%), Query Frame = 0

Query: 11  IFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNSPYGSTF 70
           +FI L  FV+ +  ++ET  R F  I +FGDS  DTGN   +S P    H++  PYG  F
Sbjct: 14  VFIFLSTFVVTN-VSSETKCREFKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENF 73

Query: 71  FHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFH-GVNFAVAGSTAINHGFFVR 130
           FHH T R+S+GRL+IDF+A+ L LP +PP+ Y   N +F  GVNFAV G+TA+   F   
Sbjct: 74  FHHPTGRFSNGRLIIDFIAEFLGLPLVPPF-YGSHNANFEKGVNFAVGGATALERSFLED 133

Query: 131 NNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGVNDYAYA- 190
             +    T  S+  QL  F + L +       + + C+   ++AL  +GEIG NDY YA 
Sbjct: 134 RGIHFPYTNVSLGVQLNSFKESLPSI----CGSPSDCRDMIENALILMGEIGGNDYNYAF 193

Query: 191 -VGSSITD-DTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPVTDRDD 250
            V   I +   +  L + +++  +  L+  G +  +V G  P GC  L ++    ++ ++
Sbjct: 194 FVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEE 253

Query: 251 ----IGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGFR 310
                GC+K LN     H   L+A L  L++ +P   IIYADY+NA   + + P+K+GF 
Sbjct: 254 YDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFGFM 313

Query: 311 ER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKGG 366
            R   ACCG G PYN+ +   CG   V SC  P +Y+ WDGVH+TEA Y+ + +  + G 
Sbjct: 314 NRPLSACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLMAEGILNGP 371

BLAST of Cp4.1LG20g03220 vs. TAIR 10
Match: AT2G27360.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.0e-61
Identity = 129/381 (33.86%), Postives = 196/381 (51.44%), Query Frame = 0

Query: 3   VALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVS 62
           +A   C  +        L +   ++T  R F  I +FGDS TDTGN   +S P      +
Sbjct: 1   MASQDCHMLLSFFISTFLITVVTSQTRCRNFKSIISFGDSITDTGNLLGLSSPNDLPESA 60

Query: 63  NSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAI 122
             PYG TFFHH + R+SDGRL+IDF+A+ L +P +PP+   K  +   GVNFAV G+TA+
Sbjct: 61  FPPYGETFFHHPSGRFSDGRLIIDFIAEFLGIPHVPPFYGSKNGNFEKGVNFAVGGATAL 120

Query: 123 NHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIGV 182
                         +  S+  QL     F E+       +   C+   ++A   +GEIG 
Sbjct: 121 ECSVLEEKGTHCSQSNISLGNQL---KSFKESLPYLCGSSSPDCRDMIENAFILIGEIGG 180

Query: 183 NDYAYAVGSSITDDTIRK---LGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLA 242
           NDY + +      + +++   L + +++  +  L+  GA+  +V G  P GC    ++L 
Sbjct: 181 NDYNFPLFDRKNIEEVKELVPLVITTISSAISELVDMGARTFLVPGNFPLGCSVAYLTLY 240

Query: 243 PVTDRDD----IGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKN 302
              ++++     GC+  LN+ +  HN  LQA L+ LR  +P   IIY DY+N    +M+ 
Sbjct: 241 ETPNKEEYNPLTGCLTWLNDFSVYHNEQLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQE 300

Query: 303 PSKYGFRER-FKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVH 362
           PSK+G  +R   ACCG+G PYNF     CG   V  C  P +Y+NWDG+H+TEA YK + 
Sbjct: 301 PSKFGLMDRPLPACCGLGGPYNFTFSIKCGSKGVEYCSDPSKYVNWDGIHMTEAAYKWIS 360

Query: 363 DMFIKGGLTHPALS-NLLDMK 375
           +  + G    P  + + LD K
Sbjct: 361 EGVLTGPYAIPPFNWSCLDSK 378