Cp4.1LG20g03200 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g03200 vs. ExPASy Swiss-Prot
Match: Q84P23 (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 568.5 bits (1464), Expect = 7.7e-161
Identity = 323/565 (57.17%), Postives = 398/565 (70.44%), Query Frame = 0

Query: 24  NAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNA------ 83
           N +  +D  SGF  +T I+ SLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 84  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 143
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 144 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKL--PKLPLGTVLIDSPAFLSM 203
           S+GVV+SPANPIGS+SE+SHQ+ +S+PVIAFATS T  KL    LPLGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 204 MTEINRSDGIA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 263
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 264 SEVNNGETEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVT 323
             VN       +V L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK+ VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 324 YIPVSPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 383
            +PVSPPL+VAL KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 384 ESTAGAARSMGPEESSNTKSVGRLSENMEAMI---------------------------- 443
           ES+  AA + GPEE     SVGR+SENMEA I                            
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 444 -----ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 503
                A+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 504 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEA--------QVAPYKKIRRVSFIDAI 532
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EA        QV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

BLAST of Cp4.1LG20g03200 vs. ExPASy Swiss-Prot
Match: Q7F1X5 (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.0e-144
Identity = 288/544 (52.94%), Postives = 373/544 (68.57%), Query Frame = 0

Query: 16  MADRNPSLNAAHF-VDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPP 75
           MADR P        +D RSGFC  T+IF S R P  +PP S P++   +A SLL SS  P
Sbjct: 1   MADRAPPPPPPPAGIDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLP 60

Query: 76  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 135
              A LVD+ +G+ +SY  FL  +R+LA  L     L  G VA ++AP+ LEVPVL FAL
Sbjct: 61  GRPA-LVDAATGIAISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFAL 120

Query: 136 LSLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSM- 195
           +S+G V+SPANP+ +  E +HQ+ LS+PV+AFA    A+KLP+  +  V+I S  +  + 
Sbjct: 121 MSIGAVVSPANPVSTPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEYGRLA 180

Query: 196 MTEINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNG 255
            ++  R+   A V V QSD+AA+LYSSGTTGRVK V ++HRNLI + S       +V   
Sbjct: 181 ASDGRRAAAPAAVAVKQSDTAAVLYSSGTTGRVKAVAITHRNLIALMSLHADNREKVARE 240

Query: 256 ETE------PRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVT 315
             E      P  V+L  +PLFHVFGF+M+LR++S GET VLM++FDF+  L A+E++ VT
Sbjct: 241 AAEAGEEPPPPAVTLLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVT 300

Query: 316 YIPVSPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 375
            +P +PP++VA+ K E   + DLSSL V+G GGAPLG+EV E+F    P VE++QGYGLT
Sbjct: 301 LLPAAPPVLVAMVKYEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLT 360

Query: 376 ESTAGAARSMGPEESSNTKSVGRLSENMEAMI------------ATTETLHPDGWLKTGD 435
           ES+   A ++GPEES    SVG+L  +++A I            AT  T+  +GWLKTGD
Sbjct: 361 ESSGAVAATVGPEESKAYGSVGKLGSHLQAKIVDPSTGYVGDDEATAATVDSEGWLKTGD 420

Query: 436 LCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPDEDAGEIPM 495
           LCYF+ DGFLYIVDRLKELIKYK YQVPPAELEH+LQS+P I DAAVIPYPDE+AGE+PM
Sbjct: 421 LCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSHPGIADAAVIPYPDEEAGELPM 480

Query: 496 AYVVRKPGSNITE--------AQVAPYKKIRRVSFIDAIPKSPAGKILRRELTQHARSHG 532
           A++VR+PGSNIT+         QVAPYKK+RRV+F+ AIPKSPAGKILRREL Q A S G
Sbjct: 481 AFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVTAIPKSPAGKILRRELVQQALSMG 540

BLAST of Cp4.1LG20g03200 vs. ExPASy Swiss-Prot
Match: Q8RU95 (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 461.5 bits (1186), Expect = 1.3e-128
Identity = 267/558 (47.85%), Postives = 347/558 (62.19%), Query Frame = 0

Query: 32  RSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPP---PSNAAVLVDSNSGVH 91
           RSGFC  T+ F SLR    +PP   PL+V  +A SLL S+PP         LVD+ +G+ 
Sbjct: 41  RSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGIA 100

Query: 92  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 151
           +SY  F+ ++R LA  L     L  G VA +++P+ L+V VLYFAL+S+GVV+SPANP  
Sbjct: 101 VSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPAS 160

Query: 152 SDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDGIA---D 211
           +  E +HQ+RLS+P IAF     A++LP+  +  V+I S  F   +   + + G A    
Sbjct: 161 TADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVF-DRLASASAAGGWAAPPA 220

Query: 212 VKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNN------------- 271
           V + Q  +AA+LYSSGTTGRVK V ++HRNLI   S+ N +   V               
Sbjct: 221 VAMKQPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKPPP 280

Query: 272 -GETEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPV 331
              + P  V+L  LPLFHV GF +L R IS GET V+M++FD      AVE++ VT +  
Sbjct: 281 PSPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKLSA 340

Query: 332 SPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTA 391
           +PP+VVAL KS+   + DLSSL  +  GGAPLG+EV ++F    P V+I+Q YGLTEST 
Sbjct: 341 APPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTESTG 400

Query: 392 GAARSMGPEESSNTKSVGRLSENMEAMI-------------------------------- 451
             A   GPEES+   SVGRL+  ++A I                                
Sbjct: 401 PVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDP 460

Query: 452 -ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEII 511
            AT  T+ PDGWLKTGDLCYF+ DG+LY+VDRLKELIKYK YQVPPAELEH+LQS PEI 
Sbjct: 461 EATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIA 520

Query: 512 DAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQ--------VAPYKKIRRVSFIDAIPKSP 529
           DAAV+PYPDE+AG++PMA+VVR+PG+ +TE Q        VAPYKK+RRV+F++AIPKSP
Sbjct: 521 DAAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVRRVAFVNAIPKSP 580

BLAST of Cp4.1LG20g03200 vs. ExPASy Swiss-Prot
Match: Q69RG7 (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 9.2e-114
Identity = 243/547 (44.42%), Postives = 336/547 (61.43%), Query Frame = 0

Query: 32  RSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNAAV------LVDSNS 91
           R+G+C  TK FRSLRPP+ +PP   PLS  E A SLL  S   S++++      LVD+ +
Sbjct: 16  RTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSSSSSSLLPANPALVDAAT 75

Query: 92  GVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPAN 151
           G  +S+  FL ++R LA  L++   L  G VAF+LAP  L+VPVLYFALLS+G V+SPAN
Sbjct: 76  GEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPAN 135

Query: 152 PIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDG--- 211
           P  + +E+S  + LS   +AFA SSTA+KLP      VL+DSP F S++ +  ++ G   
Sbjct: 136 PALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQEP 195

Query: 212 IADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRQVSL 271
           +  V V QS++AAI YSSGTTGRVK   L HR+ I + +  + L ++     T      L
Sbjct: 196 LPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAREVRT------L 255

Query: 272 CLLPLFHVFGFVMLLRAISRGETLVL----MQKFDFVEMLSAVEKFGVTYIPVSPPLVVA 331
              P+FH  GF+ +L+ ++ G T V+    + +     ++ A E++ V  +  SPP+V+ 
Sbjct: 256 LGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVVLG 315

Query: 332 LAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMG 391
           + K     +  L +L+ + CGGAPL    IE+F ++ P V++  GYG TE+  G +R + 
Sbjct: 316 MTKQ----RCRLPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEA-GGISRMIS 375

Query: 392 PEESSNTKSVGRLSENMEAMI---------------------------------ATTETL 451
            EE ++  S GR++EN+E  I                                 A   T 
Sbjct: 376 QEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANATTF 435

Query: 452 HPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPY 511
           + +GWLKTGDLCY D DGFL++VDRLKELIKYKAYQVPPAELE +L S P+I+DAAV+PY
Sbjct: 436 NSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELELVLHSLPQIVDAAVMPY 495

Query: 512 PDEDAGEIPMAYVVRKPGSNITEA--------QVAPYKKIRRVSFIDAIPKSPAGKILRR 525
           P E+AG+IP+A VV++PGS +TEA        QVAPYKKIR+V F+D+IPKSP+GKILRR
Sbjct: 496 PHEEAGQIPVALVVKQPGSKLTEAEVMYNVAKQVAPYKKIRKVLFVDSIPKSPSGKILRR 551

BLAST of Cp4.1LG20g03200 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.3e-104
Identity = 231/553 (41.77%), Postives = 328/553 (59.31%), Query Frame = 0

Query: 29  VDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 88
           VD RSG+C    IF S R P+ +P  +  + VT        SS         +D+ +G H
Sbjct: 9   VDERSGYCKSNSIFYSKREPVQLPQ-NHSVDVTTFI-----SSRAHHGKIAFIDAATGRH 68

Query: 89  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 148
           L++    R + ++A+ L A+  +  G V  +L+P S+  PV+  A++SLG +I+  NP+ 
Sbjct: 69  LTFPQLWRAVDSVATCLSAM-GIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLN 128

Query: 149 SDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDGIADV-- 208
           +  EI+ QI  S+PV+AF      SK+    L  V+ID     S+   +N    + ++  
Sbjct: 129 TPREIAKQITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMR 188

Query: 209 ----------KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETE 268
                     +V+Q D+A +LYSSGTTG  KGV+ SH+NLI +  +   +LS    G  +
Sbjct: 189 KEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQT---ILSRF--GTED 248

Query: 269 PRQVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 328
                +C +P+FH++G     +  +S G T+V++ KF+  EMLSA+EK+  TY+P+ PP+
Sbjct: 249 GEHTFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPI 308

Query: 329 VVALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGA 388
           ++AL K  + + AKYDLSSLQ +  GGAPL KEVIE F +  P V I+QGYGLTEST   
Sbjct: 309 LMALLKNANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIG 368

Query: 389 ARSMGPEESSNTKSVGRLSENMEAMI---------------------------------A 448
           A +   +ES    + G LS +MEA I                                 A
Sbjct: 369 ASTDCLQESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEA 428

Query: 449 TTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDA 508
           T+ T+  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL S+PEI DA
Sbjct: 429 TSSTIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDA 488

Query: 509 AVIPYPDEDAGEIPMAYVVRKPGSNITEAQ--------VAPYKKIRRVSFIDAIPKSPAG 526
           AVIPYPD++AG+ PMAYVVRK GSN++E+         VAPYK+IR+V+F+ +IPK+P+G
Sbjct: 489 AVIPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSG 548

BLAST of Cp4.1LG20g03200 vs. NCBI nr
Match: XP_023519270.1 (4-coumarate--CoA ligase-like 9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 516/557 (92.64%), Postives = 516/557 (92.64%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSNITEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. NCBI nr
Match: XP_023000795.1 (4-coumarate--CoA ligase-like 9 [Cucurbita maxima])

HSP 1 Score: 957 bits (2474), Expect = 0.0
Identity = 508/557 (91.20%), Postives = 514/557 (92.28%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVD+RSGFCPQTKIFRSLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGV+ISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. NCBI nr
Match: KAG6584140.1 (4-coumarate--CoA ligase-like 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 509/557 (91.38%), Postives = 513/557 (92.10%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSNITEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. NCBI nr
Match: KAG7019731.1 (4-coumarate--CoA ligase-like 9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 508/557 (91.20%), Postives = 513/557 (92.10%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSNITEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. NCBI nr
Match: XP_022923628.1 (4-coumarate--CoA ligase-like 9 [Cucurbita moschata])

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 508/557 (91.20%), Postives = 512/557 (91.92%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSNITEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. ExPASy TrEMBL
Match: A0A6J1KJC1 (4-coumarate--CoA ligase-like 9 OS=Cucurbita maxima OX=3661 GN=LOC111495139 PE=4 SV=1)

HSP 1 Score: 957 bits (2474), Expect = 0.0
Identity = 508/557 (91.20%), Postives = 514/557 (92.28%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVD+RSGFCPQTKIFRSLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGV+ISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. ExPASy TrEMBL
Match: A0A6J1EA56 (4-coumarate--CoA ligase-like 9 OS=Cucurbita moschata OX=3662 GN=LOC111431280 PE=4 SV=1)

HSP 1 Score: 953 bits (2463), Expect = 0.0
Identity = 508/557 (91.20%), Postives = 512/557 (91.92%), Query Frame = 0

Query: 16  MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 75
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 76  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 135
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 136 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 195
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 196 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 255
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 256 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 315
           EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 316 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 375
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 376 SMGPEESSNTKSVGRLSENMEAMI---------------------------------ATT 435
           SMGPEESSNTKSVGRLSENMEAMI                                 ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 436 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 495
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 496 IPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGKI 531
           IPYPDEDAGEIPMAYVVRKPGSNITEAQV        APYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

BLAST of Cp4.1LG20g03200 vs. ExPASy TrEMBL
Match: A0A1S4DV63 (4-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1)

HSP 1 Score: 811 bits (2094), Expect = 7.27e-292
Identity = 430/558 (77.06%), Postives = 468/558 (83.87%), Query Frame = 0

Query: 16  MADRNPSLNAA-HFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPP 75
           MA RN + NAA H +D+RSGFCP TKIF SLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 76  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 135
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 136 LSLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 195
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 196 TEINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 255
           TE N SDG+ D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 256 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPP 315
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKF V YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 316 LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 375
           LVVA+AKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 376 RSMGPEESSNTKSVGRLSENMEAMI---------------------------------AT 435
           R+M PEE SNTKSVGRLS +MEA I                                 AT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 436 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 495
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 496 VIPYPDEDAGEIPMAYVVRKPGSNITEAQV--------APYKKIRRVSFIDAIPKSPAGK 531
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQV        APYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

BLAST of Cp4.1LG20g03200 vs. ExPASy TrEMBL
Match: A0A5D3BKJ5 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004520 PE=4 SV=1)

HSP 1 Score: 806 bits (2083), Expect = 5.14e-290
Identity = 430/569 (75.57%), Postives = 468/569 (82.25%), Query Frame = 0

Query: 16  MADRNPSLNAA-HFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPP 75
           MA RN + NAA H +D+RSGFCP TKIF SLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 76  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 135
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 136 LSLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 195
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 196 TEINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 255
           TE N SDG+ D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 256 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPP 315
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKF V YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 316 LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 375
           LVVA+AKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 376 RSMGPEESSNTKSVGRLSENMEAMI---------------------------------AT 435
           R+M PEE SNTKSVGRLS +MEA I                                 AT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 436 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 495
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 496 VIPYPDEDAGEIPMAYVVRKPGSNITEAQV-------------------APYKKIRRVSF 531
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQV                   APYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

BLAST of Cp4.1LG20g03200 vs. ExPASy TrEMBL
Match: A0A5A7UM03 (4-coumarate-CoA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001590 PE=4 SV=1)

HSP 1 Score: 803 bits (2073), Expect = 1.71e-288
Identity = 429/569 (75.40%), Postives = 467/569 (82.07%), Query Frame = 0

Query: 16  MADRNPSLNAA-HFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPP 75
           MA RN + NAA H +D+RSGFCP TKIF SLRPPL +PPLSQPLSVT+HA SLLQSSP P
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSPLP 60

Query: 76  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 135
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 136 LSLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 195
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 196 TEINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 255
           TE N SDG+ D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 256 TEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPP 315
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKF V YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 316 LVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 375
           LVVA+AKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 376 RSMGPEESSNTKSVGRLSENMEAMI---------------------------------AT 435
           R+M PEE SNTKSVGRLS +MEA I                                 AT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 436 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 495
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN EIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNSEIIDAA 480

Query: 496 VIPYPDEDAGEIPMAYVVRKPGSNITEAQV-------------------APYKKIRRVSF 531
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQV                   APYKKIRRVSF
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 540

BLAST of Cp4.1LG20g03200 vs. TAIR 10
Match: AT5G63380.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 5.5e-162
Identity = 323/565 (57.17%), Postives = 398/565 (70.44%), Query Frame = 0

Query: 24  NAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNA------ 83
           N +  +D  SGF  +T I+ SLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 84  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 143
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 144 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKL--PKLPLGTVLIDSPAFLSM 203
           S+GVV+SPANPIGS+SE+SHQ+ +S+PVIAFATS T  KL    LPLGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 204 MTEINRSDGIA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 263
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 264 SEVNNGETEPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVT 323
             VN       +V L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK+ VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 324 YIPVSPPLVVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 383
            +PVSPPL+VAL KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 384 ESTAGAARSMGPEESSNTKSVGRLSENMEAMI---------------------------- 443
           ES+  AA + GPEE     SVGR+SENMEA I                            
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 444 -----ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 503
                A+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 504 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEA--------QVAPYKKIRRVSFIDAI 532
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EA        QV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

BLAST of Cp4.1LG20g03200 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 379.0 bits (972), Expect = 6.2e-105
Identity = 241/554 (43.50%), Postives = 322/554 (58.12%), Query Frame = 0

Query: 29  VDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 88
           VD +SGFC  T IF S R P+ +PP +Q L VT        +S P     V VD+ +G  
Sbjct: 21  VDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVTGRR 80

Query: 89  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 148
           LS+      +  +A  L AL  +  G V  IL+P S+  P++  +++SLG +I+ ANPI 
Sbjct: 81  LSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPIN 140

Query: 149 SDSEISHQIRLSQPVIAFATSSTASKL---PKLPLGTVLID-----SPAF---------L 208
           +  EIS QI  S+PV+AF T    SKL       L  VL+D     S ++         L
Sbjct: 141 TSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRL 200

Query: 209 SMMTEINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVN 268
             M E   S+     +V+Q D+AA+LYSSGTTG  KGV+LSHRNLI         L +  
Sbjct: 201 ETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--------LVQAY 260

Query: 269 NGETEPRQVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIP 328
                  Q ++C +P+ H+FGF       I+ G T+V++ KFD  ++LSAVE    +Y+ 
Sbjct: 261 RARFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLS 320

Query: 329 VSPPLVVALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTE 388
           + PP+VVA+    +E+ +KYDLSSL  +  GGAPL +EV EKF +  PKV+I+QGYGLTE
Sbjct: 321 LVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTE 380

Query: 389 STAGAARSMGPEESSNTKSVGRLSENMEAMI----------------------------- 448
           STA AA     EE+    + G L+ N+E  I                             
Sbjct: 381 STAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYF 440

Query: 449 ----ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNP 508
               AT  T+  +GWLKTGDLCY D DGF+++VDRLKELIK   YQV PAELE LL ++P
Sbjct: 441 KNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHP 500

Query: 509 EIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEA--------QVAPYKKIRRVSFIDAIP 522
           EI DAAVIP PD  AG+ PMAY+VRK GSN++E+        QV+PYKKIR+V+F+ +IP
Sbjct: 501 EIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIP 559

BLAST of Cp4.1LG20g03200 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 378.3 bits (970), Expect = 1.0e-104
Identity = 225/553 (40.69%), Postives = 329/553 (59.49%), Query Frame = 0

Query: 29  VDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 88
           V+ RSGFC     F S R P+ +PP +  L VT        SS         +D+++G +
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQN 63

Query: 89  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 148
           L++    R + ++A  L  +  +  G V  +L+P S+  PV+  +++SLG +I+  NP+ 
Sbjct: 64  LTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 149 SDSEISHQIRLSQPVIAFAT-------SSTASKLPKLPLGTVLIDSPAFLSMMTEINR-- 208
           + +EI+ QI+ S PV+AF T       S+ A KLP + +    +DS   +  + E+ +  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 183

Query: 209 -SDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNN-GETEP 268
            S      +VDQ D+A +LYSSGTTG  KGV+ SHRNLI +      + + VN  G  + 
Sbjct: 184 PSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAM------VQTIVNRFGSDDG 243

Query: 269 RQVSLCLLPLFHVFGFVMLLRA-ISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPLV 328
            Q  +C +P+FH++G        ++ G T++++ KF+  EM+SA+ K+  T +P+ PP++
Sbjct: 244 EQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPIL 303

Query: 329 VALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 388
           VA+     ++ AKYDLSS+  + CGGAPL KEV E F +K P V+I+QGYGLTEST   A
Sbjct: 304 VAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGA 363

Query: 389 RSMGPEESSNTKSVGRLSENMEAMI---------------------------------AT 448
            +   EES    + G+LS +ME  I                                 AT
Sbjct: 364 STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEAT 423

Query: 449 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 508
           + TL  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++PEI DAA
Sbjct: 424 SSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAA 483

Query: 509 VIPYPDEDAGEIPMAYVVRKPGSNITE--------AQVAPYKKIRRVSFIDAIPKSPAGK 527
           VIP+PD++ G+ PMAYVVRK GS+++E         QVAPYK+IR+V+F+ +IPK+P+GK
Sbjct: 484 VIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGK 543

BLAST of Cp4.1LG20g03200 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 365.9 bits (938), Expect = 5.4e-101
Identity = 225/554 (40.61%), Postives = 318/554 (57.40%), Query Frame = 0

Query: 29  VDIRSGFCPQTKIFRSLRPPLIIPP-LSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGV 88
           VD RSGFC     F S R PL +PP LS+ ++          SS P       +D+ +G 
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTT-------FISSQPHRGKTAFIDAATGQ 69

Query: 89  HLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPI 148
            L+++   R +  +A  L     +  G V  IL+P S+ +PV+  +++SLG V + AN +
Sbjct: 70  CLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTL 129

Query: 149 GSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAF------------LSMMT 208
            +  EIS QI  S P + F T   A KLP + +  VL D   +            LS M 
Sbjct: 130 NTSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMV 189

Query: 209 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 268
           +   S      +V+Q D+A +LYSSGTTG  KGV+ SHRNL          ++   +   
Sbjct: 190 KKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNL-------TAHVARFISDNL 249

Query: 269 EPRQVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPP 328
           +   + +C +P+FH +G +   +  ++ G T+V++++F   +M+ AVEK   T + ++PP
Sbjct: 250 KRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPP 309

Query: 329 LVVALAKSE--LVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAG 388
           ++VA+      + AKYDLSSL+ + CGGAPL KEV E F +K P V+I+QGY LTES  G
Sbjct: 310 VLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGG 369

Query: 389 AARSMGPEESSNTKSVGRLSENMEAMI--------------------------------- 448
            A +   EES    + G L+ ++EA I                                 
Sbjct: 370 GAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQE 429

Query: 449 ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIID 508
           AT ET++ +GWLKTGDLCY D DGFL++VDRLKELIKYK YQVPPAELE LL ++P+I+D
Sbjct: 430 ATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILD 489

Query: 509 AAVIPYPDEDAGEIPMAYVVRKPGSNITE--------AQVAPYKKIRRVSFIDAIPKSPA 526
           AAVIP+PD++AG+ PMAYVVRK  SN++E         QVAPYKKIR+VSFI++IPK+ +
Sbjct: 490 AAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTAS 548

BLAST of Cp4.1LG20g03200 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 354.0 bits (907), Expect = 2.1e-97
Identity = 217/557 (38.96%), Postives = 315/557 (56.55%), Query Frame = 0

Query: 22  SLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPSNAAVLV 81
           S  ++  +D R+GFC     F S R PL +P   + L +T        SS         +
Sbjct: 4   SQRSSSLIDPRNGFCTSNSTFYSKRKPLALPS-KESLDITTFI-----SSQTYRGKTAFI 63

Query: 82  DSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVI 141
           D+ +   +S++     +  +A  L     +  G V  +L+P ++ +P++  +++SLG V+
Sbjct: 64  DAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVL 123

Query: 142 SPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVL------IDSPAFLSM-- 201
           + ANP+ + SEI  QI  S P +AF T   A K+    +  VL      +  P  L +  
Sbjct: 124 TTANPLNTASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVG 183

Query: 202 -MTEINRSDGIADV---KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSE 261
            +TE+ + +        +V + D+A +LYSSGTTGR KGV  SH NLI        +   
Sbjct: 184 NLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIA------HVARY 243

Query: 262 VNNGETEPRQVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTY 321
           +     +P+Q  +C +PLFH FG +  +L  ++ G T+V++ +FD  EM++AVEK+  T 
Sbjct: 244 IAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATT 303

Query: 322 IPVSPPLVVALAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGL 381
           + + PP++V +     +++ KYD+S L+ + CGGAPL KEV + F +K P V++ QGY L
Sbjct: 304 LILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYAL 363

Query: 382 TESTAGAARSMGPEESSNTKSVGRLSENMEAMIATTET---------------------- 441
           TES    A     EES    +VG LS  +EA I    T                      
Sbjct: 364 TESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKG 423

Query: 442 --------LHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPE 501
                   +  +GWLKTGDLCY D+DGFL+IVDRLKELIKYK YQVPPAELE LL ++P+
Sbjct: 424 YFRNEEEIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPD 483

Query: 502 IIDAAVIPYPDEDAGEIPMAYVVRKPGSNITE--------AQVAPYKKIRRVSFIDAIPK 526
           I+DAAVIP+PD++AG+ PMAYV RKP SN+ E         QVAPYKKIR+V+FID+IPK
Sbjct: 484 ILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPK 543