Cp4.1LG20g03110 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g03110
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionUbiquitin-like modifier-activating enzyme 5
LocationCp4.1LG20: 1712984 .. 1720400 (-)
RNA-Seq ExpressionCp4.1LG20g03110
SyntenyCp4.1LG20g03110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCTGAACAGAAAGGCGATCTGGGCAGCACGCCGATATCTTTGAGCGCATTGTTTATGTATGGCTGATTGTCATAAATAAAATAAAACGGTGAATCTAATTTAAGAGGATACCATCTTAATCTTGAGAATTTCGTTGAATTTCTTTGACTTTGGAGGATTTTTGTATAGAGTCAATTGGAGCTCCGTCAGAGAGAGCGAGAGTGGGAGCAGAAATGGAGGTGGAATTGGAAGAGATGTGGAACGATCTCGAATCCCTGAAGCAGTCGCTAACCGATCCGTCTCATCGGGGACCGGTTGATAAGGTACTTCTGCTTTTCAGTTCTGCATCATTTCTTTTGATCATTTAGTTCGGAATTAACTTCCTGAACAATTGTGCCTATGTTCTGGCTGGATTTTTTTTTGTTTTTTGAACTGAATCATTTCATTGTCGAATTCTTTCTGCGTGTGGTTGAACCTGTTTTACGTCCTTTTAATTTACCCGCGAATCCTGCATGTATGGTTTTGTTTTGCATGAATATTCAAGTGTATCTTTCACTATGGAGAACTGAGAACGTTCCTCTTTCATAATGTAAAGGAGATTACGTTAGTGAAGATAGTTTGTAAATGTGTTGAGTGTTTGATTATTCTAACACTGAATTCAATTGCTCTCTGGCTATGTCCAATCCAGCTTCAATTGCATTTAGAGCGTCTCACAAATCTTTCGAAGTCAGGACCAGTACGGCGATCAAAAGTTAAGGTGTCTGTTCTATGCCCTGTCATATGTTGATTTCTTCCGTTGCTTTTTAATTGAAGCTTATGATTATATTTATGAACACATGCATCAATGAAATGGTGGCAGGACATGAGTGCTGAGGTGGTAGATAGCAACCCTTACAGTAGGCTTATGGCTCTTCAGAGGATGGGCATTGTAGAAAACTATGAAAGAATACGGGAGTTTTCAGTTGCCATAGTTGTATGTACTCATCTCCTCTTTCTTTATGTGTGTTCTAGAGTTTCAATTTGAGAAGTTGACATACATGGTTATATGTCTTCCCCTCATATGATATGTTTTCACTGTGGTTTTCAACTTTCAGACATTTTGTTGTTTAATTTTTATAATGAATTTTTATGTCATTTCATTCTTCATCCACAGGGAATTGGAGGTGTTGGAAGTGTTGCAGCTGAGATGTTGACAAGGTGTGGAATAGGTCGCTTGTTGCTATATGATTACGATAAAGTGGAGCTAGCTAACATGAATAGACTCTTCTTTCGTCCCGAGCAGGTAATCATTTCAGTATTTCGTCCTCCTTTCTTACCTGGGGGTCTTGTGTGGTTTAAGAATTGGATGTGCTTGCTCTTAATTTTGATCCCTTCCTGCATTTAGTTGTATGCCTGGCTGTAAATCTACATTTTCATGTTGCTACACTCTTCATGTTCTGGAAGGTCTTTTAGTTCTAAGTTTGTACCCACATATAAGGCAGCTGAATGTAAAGTTGCTTGTTTGTTCACTAGTTTACCTTCCCAATATATTTTCAGGTTGGTATGACAAAAACTGATGCTGCAGTGCAGACTCTTGCAGATATAAACCCCGATGTTGTACTTGAGGTTAGCCTTTTATACTACTGGTCTATTACTTGCACCTTTGTTGATGAATAATAACCACAGATGACCAGTAATAGTTTGTGCATAAAAACGAATTTTCTTTGTACTTGACGCAAAATGAATGGAACTTTTATGTTTTTTTTTTCCTAATATTATGTATAATTTTCTATAATATTCATGCTATCACTAAGAATAGTTCTAAGAAAGAATATAGCTTTGGAACATTTAATTTTCTCTTCATTCTCACTATAAATCACAACATTAAACTATGCAAAATATCTGTTTCATTTTGTTCTTTTATTCCTCATTCTGATGGGTTCATTTTGTTCACAGAGCTATACGCTGAACATCACAACTGTACAAGGTTTTGAAACTTTCATGTCAAGTTTGAGAAACAAATCATTCTCCCCTACTAAAGAAGGCAGTGGAGTGGATCTTGTCTTAAGTTGTGTTGATAACTATGAAGCAAGGATGGCTGTTAATCAGGCAAGTTTCTTCAGTGTCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTGAGACTATGTACGTTGTTGTACTTTGACGTTAATATTTAAAATTTGATAGACAGGCTTGCAATGAGCTAAATCAGACATGGATGGAATCCGGTAAGCAAGAAATATTCTTATTTAGACCATTCGCCTAAGATCGAATTTTTATACGTAGTCCTCCCTTTATGCATTTATTTAGATAGATTTCAATAGAGTTAGCTTTCATAAAAAATAAAAAATTGAAAAGATGAAAAAAAATCAATAAAGTTAGCTTGAAACTGCCAAATTACGGACAGATGCATGTGGCATAGTTATATTTTCACTAACTGACTACATAACACACACAAGTGAGTTAGAACACCTAAAATCTTAATTTTGATGTATTGGATAAGTACTAGGAATTGTAAATTGATGTCAATAGAAATTTAGAAGTACAACACTCAAATTTATGAAATCATTGATTAAATGTCTCCACAGATATTTGAGGAAAACATGGAAATCTCAAGAATTGATAAAATTGTTTGAATTAATTTGTTAAGCATCATCATTAGTTTATATTAATATTATTGGTGGTTGTCACAATGACATGTCTAAGTTTTCTTGACATTTCACTGACATATTGAAGAAAAAAAATATATTCTTGCATGTCAATGTTATCGATATAATTTTTATTGACACATCAACAAAAATTTTACTTCATGGATGAGAATGATTTAAGTTACTACTGGAAAAGGTCTGGGAAGCATCTCAACGTAGATATGGGTTTTAAGTTACGACTGGAAAAGGTCTCAGAATGATTTGAATGTGAATGCGTGACAAGTGCTAATGCCTGGTACCATGGGCAGGATGATTTTCAAACTCCTTGTTAAAATAATCCGCTCTTAGGAATAGTTCTACTTTGATTTTTTAAATAAATTTATAAAGTTATTATAAAGGTGATAAATGTGAACTTACAATCACAGCTACTGTTATTTTGACTAGCCTAGTGATATTTTGAAAGCATATTTTATGCTAGCTTCTTAGTTCAAATCTAAGTAGGGCATGCGGGCGACCATTCTTTGATTAACAAAATCATACTCATATTTTATACGAAAAGATAAAAAGAATATTAAATTGTAAGTTTAGTCCTTTGACAATTTCTTTCTAATAGGTGCATGACATTTTAATTTTGAGTCAAATGTCTGTGAGTTTCTAATATGTTTAATGGGTTAGAAACCTTTAGTTACAAGCTTTCGAGAGTTTAGGAGCTTATTAGACACACTTTAAATGTTCAAAATCCTATTAGACACAAATTTGAGTTTGTGACTTATTAGATACTTTTTAAATTTTAGAGTCCTCAAGAAGTAAACTTATAACTTAAAACCTATGAAAAGAAATAATTACTTTGCGCGTTAGCACTCATTATTTACAAATTGGAGATTTGTTAAGATATTTCTTTGCACATATATTGCTAAGACTTCGCAAATTGGAGCTTTGTTTAGGTGTTTCCGAGGATGCTGTCTCTGGTCATATTCAGTTGCTTATTCCTGGTGAAACCGCCTGTTTTGCTTGTGCACCACCTCTGGTATTCCTCCTCTCTAATCAAGATCTTGAATGTTTGGAGATTTCTCAATTTTAGTATAGATGTTGTATACTTATATTAACTACAAAACCCATGTTCTGCTGTTTCTTAACTGCAATAACTTTGTTTATGTGGTTCACCTGACGGTGTACCTTCCTCATCATCTCTTTTTTAATGTCATTGCTCATTGGTGGAAGGATTTATTCATTTATTTTCTTTGTTCAGGTTGTAGCATCTGGCATAGATGAACGAACACTCAAACGAGAAGGGGTTTGTGCTGCATCTCTACCTACAACAATGGTAAGGATTTTCTCGTTTCTGATACAAGTCCCAAACTGCACGTGTTAATAGGGAAGCCGGAAGGCTGTTCCTGATCCTGTCAGGGGAGATACACACCATCTCTCCTTTAATCGAACACAGAATTCCTAGGTTTTCTTATACAAATTATGTTCTAAAACCATCAACGAACATGTTTACATGTTTAGCATTTAATAAGTAACAAAAAGGAAATGTGGAAATAATTATTGTTTTGGAAATTATTTAAGAACTAGTCCAAGGTTAGCTAGGTCTGTCAATATGTTGGCTGCTTGCGTCCGTAGGCATCAACATTTGTATGGAGGGAACAAAAAAAAATAATTGCTGTTACTTGCATCAGATAATTTGTTTTGCTAATATGTTTTTTTCTTCGGTACAAGTGCCGTTGCTAACTTGCATACAGAACCAGGGGTGGAAACTGTACTAGATCCATGAGAAAACCATAAACCTTTTTGTAATGACGTTTTTTGTTACTAGGTCCACATTGCCTATTCAATACACGGTATATTTTGGGAATTGTTATTAAGACGCTCCCATCATAGGAAAATCGCTGTTAGCATAGCAAATGTGTGTTTGGAAAGTCAAAAGCACTTTCAAACAATATTTCATGTTTTTCAGCTTCGTTCTGAAAAAATTTCAGCAAATTTGAAAGTAGTTGAAAAGGTGTCAAGTATTTTTTGAGAAAGAACTTAATTCTTGTTTTCTTCTTCCAAAAATATCTGCCAGTAGGTATATAAGAACACTACTCTAAATACCACTCTTATTAGAACTTCAAAATATGTTCCACAAATGATGACATAATTCAAGATTGGCTAACACTAACTAATGTATCATATTGACATCTATGATTTTGCTTTTTTATTATATTTTTCTAATTGTATAGACTAATTTGAAATGATTTTCAGGGAGTTGTTGCAGGCCTATTGGTGCAAAACACCCTCAAGTTCCTGCTAAAATTTGGGCATGTTTCTCCTTACTTGGTGAGTATAACTTGCTCCTCTAATCTTTATTCATTAAAAAGGCATGATTACGAAGACAGAGACCATATTAATGGGCATTGTCAATCAATCAAAAACTAACCAACCATTTGATCAACTAGAAAGCAACTTAACAATTCTAAAACTAATTAGCATTAAGTGAATTGATTCAACAAAATAGAAAAGCTCAAACAGTAAATGTTAAGAGAATTTGAATGTTCCCCGCTGGTCATCAATTCCCTACCCTAAAAAGAGCTCATGTTAGAGTTTAAGATACAGCTGCGGAAAATACCTAAAACAATCAAGGATCCTTCTAAACAGAACTTCCTCTTATAATTTGGGAAGATTAATAAAAGTCTACCTTCAGCGCATTCAGTTTATGATATTCGCATTTGAAGGACAATATGAAAGAAGTTGAAAGGTTGAAGGCACGAACTGCACATTTATGATGATGCGTGCAATGGCAGTGCCTTGCGTCAAAATCTTTTAAAAAATTCTGGAACCAAACTAATGGCAGTTTCTTCCCCTAAACTTTGGGTTGAACACAAAAAAAAAAAAAAAAAAACNACTAATCATTGTATCAATTAAAACCTTAAACTTTGTAAGTGAATCAATTTGAATTCTCGGTTATGATTCTACTTTAAAATCTATAATGTATAATTTTCACACAAATTAAAAGACTTTTAAATGCATGGATTAAGCGTAATATTATGTGATAAATTTTCTTCCTATTTTGGTTGGATTTTGCGAGAAAATTACTCTTTATTTAGGTGGTTTTGAGTTAGATTTTAGATGCAATCAAGTGAAAATAGTTATAAATCGGTTTAAATTGATGCAACTATTAGTTTGAGATTTTAATTGATGTATCCTCCAAAATGTAGGAGCACAAATTGATTTGTTTCCGAAATAAGTCGAGTATGTTTGTGCTTGTTTGGCTCCCTCGTCCTTTTTTTCTTACTGGTTATATTCATTGCAGGGATATAATTCTCTAAAAGACTTCTTCCCGACCATGGAAATGAGGCCCAATCCTCAGTGTTCAAATGTGGTTTGTCTGGAAAGACAGGTAACTCCGACCGTTTTTTAAGTCGGTTAATCTCAATTTTTGAATTCCTATGGATTATTAATAATATGTGTGGTGCAGAAGGAATACGTTATTGCAAAACCTGCACGTGATGCTGCAGCTAAGGCAACGATGGAAGCAGAAGCATCGACGGTTGAAGAAATCCCACTGCATGCTGATAATGAATGGAACATAAGGTGTTTGCTCTAACTCCTAACATTATACATGATGTAGTCTTGATTCCTCACTTGATTATCTTTTCAATCATAGATTAAATACAGTTGAGAAATTCTAGTTAAAATTTGATATAGATATTTTAAATCGATAGCCTTGTTTTAACTTTGAAGAGATAAAATCTCTGCCTTTTGGTCTTGTCAGCGCTACCAAAATTCATCAAGAATTGATGCAGAGTCCTACAGATTATAGACACTTAGATTCGAGATTTAGCATTATCTAAAGAGTCTATTATGATTCCACCTCCGGTGTCTTAATTTATTTACTGTTGATTTTCAGTGTTGTTGATGAAGTTGAGATTGAAATCGTGGGAGCAACCACTTCAGGTGATCTTTTATGCATTTATTTTAGTTTCAGAATTTTCTTTCCGTTCTCTTTGCCTCCCGCCCAGATCATCGTCTTCTCGGAGCCTTGGTTTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAAATCTTGAGGGAAAGCCTGAAGGAAAAGTCCAAAGAGGACAATATCTGCTAGTGGTGGGTTTAGGCCGTTACAGGCTTTGTTTCACTTGGTAATACAACTTGATATTGATCTTGAAGTTAGATTCAACTGGTAGAAATTAGACGTCATTTGTTGATTATTTTTTATGGCAGGAATTTGGTTCTTTGCTGTGGCAAGGATATATTTAATCTATTTGATATTCACTTCGATTTGTTTTTCCTGGCAGATGCCCTTCCCGAAGGTCTTGTTCGTGAGCTTCCAATTGCAGATGAATCGCAGCATCCGGCTCCCACAGAATCCGCAGCCCCTTCTTCAGATGATCTTGAAGATCTGAGGAGACAACTTGAGGCTCTTAATTCTTGATCTCCAAATGTTTTCAGCTCTCGATCTCGTTTGGATATCACCATTTTGCTCCCCTCTGCAAATTGTATGTGTTCATCCAATCTGAATTATAGCTTACAATAGCACTCTAGGGAGTTCAAAGAGAGAGAAGCTATTCCATTTCAGGGCTAATGCTTCAAATAAACTATTTTCTCGACTGGATTCCTCCTTGTTTTTCTTTTGACAACAAATATTATTCTTCAAATTAAGAGGCTTACTTCTTCCAGCATTTTTTTTGGTTGGGAATTATACAACCATCAAAGATCCGTCCCACGTCGAACATTAGAGCAGC

mRNA sequence

CATTCTGAACAGAAAGGCGATCTGGGCAGCACGCCGATATCTTTGAGCGCATTGTTTATAGTCAATTGGAGCTCCGTCAGAGAGAGCGAGAGTGGGAGCAGAAATGGAGGTGGAATTGGAAGAGATGTGGAACGATCTCGAATCCCTGAAGCAGTCGCTAACCGATCCGTCTCATCGGGGACCGGTTGATAAGCTTCAATTGCATTTAGAGCGTCTCACAAATCTTTCGAAGTCAGGACCAGTACGGCGATCAAAAGTTAAGGACATGAGTGCTGAGGTGGTAGATAGCAACCCTTACAGTAGGCTTATGGCTCTTCAGAGGATGGGCATTGTAGAAAACTATGAAAGAATACGGGAGTTTTCAGTTGCCATAGTTGGAATTGGAGGTGTTGGAAGTGTTGCAGCTGAGATGTTGACAAGGTGTGGAATAGGTCGCTTGTTGCTATATGATTACGATAAAGTGGAGCTAGCTAACATGAATAGACTCTTCTTTCGTCCCGAGCAGGTTGGTATGACAAAAACTGATGCTGCAGTGCAGACTCTTGCAGATATAAACCCCGATGTTGTACTTGAGAGCTATACGCTGAACATCACAACTGTACAAGGTTTTGAAACTTTCATGTCAAGTTTGAGAAACAAATCATTCTCCCCTACTAAAGAAGGCAGTGGAGTGGATCTTGTCTTAAGTTGTGTTGATAACTATGAAGCAAGGATGGCTGCTTGCAATGAGCTAAATCAGACATGGATGGAATCCGGTGTTTCCGAGGATGCTGTCTCTGGTCATATTCAGTTGCTTATTCCTGGTGAAACCGCCTGTTTTGCTTGTGCACCACCTCTGGTTGTAGCATCTGGCATAGATGAACGAACACTCAAACGAGAAGGGGTTTGTGCTGCATCTCTACCTACAACAATGGGAGTTGTTGCAGGCCTATTGGTGCAAAACACCCTCAAGTTCCTGCTAAAATTTGGGCATGTTTCTCCTTACTTGGGATATAATTCTCTAAAAGACTTCTTCCCGACCATGGAAATGAGGCCCAATCCTCAGTGTTCAAATGTGGTTTGTCTGGAAAGACAGAAGGAATACGTTATTGCAAAACCTGCACGTGATGCTGCAGCTAAGGCAACGATGGAAGCAGAAGCATCGACGGTTGAAGAAATCCCACTGCATGCTGATAATGAATGGAACATAAGTGTTGTTGATGAAGTTGAGATTGAAATCGTGGGAGCAACCACTTCAGATGCCCTTCCCGAAGGTCTTGTTCGTGAGCTTCCAATTGCAGATGAATCGCAGCATCCGGCTCCCACAGAATCCGCAGCCCCTTCTTCAGATGATCTTGAAGATCTGAGGAGACAACTTGAGGCTCTTAATTCTTGATCTCCAAATGTTTTCAGCTCTCGATCTCGTTTGGATATCACCATTTTGCTCCCCTCTGCAAATTGTATGTGTTCATCCAATCTGAATTATAGCTTACAATAGCACTCTAGGGAGTTCAAAGAGAGAGAAGCTATTCCATTTCAGGGCTAATGCTTCAAATAAACTATTTTCTCGACTGGATTCCTCCTTGTTTTTCTTTTGACAACAAATATTATTCTTCAAATTAAGAGGCTTACTTCTTCCAGCATTTTTTTTGGTTGGGAATTATACAACCATCAAAGATCCGTCCCACGTCGAACATTAGAGCAGC

Coding sequence (CDS)

ATGGAGGTGGAATTGGAAGAGATGTGGAACGATCTCGAATCCCTGAAGCAGTCGCTAACCGATCCGTCTCATCGGGGACCGGTTGATAAGCTTCAATTGCATTTAGAGCGTCTCACAAATCTTTCGAAGTCAGGACCAGTACGGCGATCAAAAGTTAAGGACATGAGTGCTGAGGTGGTAGATAGCAACCCTTACAGTAGGCTTATGGCTCTTCAGAGGATGGGCATTGTAGAAAACTATGAAAGAATACGGGAGTTTTCAGTTGCCATAGTTGGAATTGGAGGTGTTGGAAGTGTTGCAGCTGAGATGTTGACAAGGTGTGGAATAGGTCGCTTGTTGCTATATGATTACGATAAAGTGGAGCTAGCTAACATGAATAGACTCTTCTTTCGTCCCGAGCAGGTTGGTATGACAAAAACTGATGCTGCAGTGCAGACTCTTGCAGATATAAACCCCGATGTTGTACTTGAGAGCTATACGCTGAACATCACAACTGTACAAGGTTTTGAAACTTTCATGTCAAGTTTGAGAAACAAATCATTCTCCCCTACTAAAGAAGGCAGTGGAGTGGATCTTGTCTTAAGTTGTGTTGATAACTATGAAGCAAGGATGGCTGCTTGCAATGAGCTAAATCAGACATGGATGGAATCCGGTGTTTCCGAGGATGCTGTCTCTGGTCATATTCAGTTGCTTATTCCTGGTGAAACCGCCTGTTTTGCTTGTGCACCACCTCTGGTTGTAGCATCTGGCATAGATGAACGAACACTCAAACGAGAAGGGGTTTGTGCTGCATCTCTACCTACAACAATGGGAGTTGTTGCAGGCCTATTGGTGCAAAACACCCTCAAGTTCCTGCTAAAATTTGGGCATGTTTCTCCTTACTTGGGATATAATTCTCTAAAAGACTTCTTCCCGACCATGGAAATGAGGCCCAATCCTCAGTGTTCAAATGTGGTTTGTCTGGAAAGACAGAAGGAATACGTTATTGCAAAACCTGCACGTGATGCTGCAGCTAAGGCAACGATGGAAGCAGAAGCATCGACGGTTGAAGAAATCCCACTGCATGCTGATAATGAATGGAACATAAGTGTTGTTGATGAAGTTGAGATTGAAATCGTGGGAGCAACCACTTCAGATGCCCTTCCCGAAGGTCTTGTTCGTGAGCTTCCAATTGCAGATGAATCGCAGCATCCGGCTCCCACAGAATCCGCAGCCCCTTCTTCAGATGATCTTGAAGATCTGAGGAGACAACTTGAGGCTCTTAATTCTTGA

Protein sequence

MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVVDSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSFSPTKEGSGVDLVLSCVDNYEARMAACNELNQTWMESGVSEDAVSGHIQLLIPGETACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGYNSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHADNEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQLEALNS
Homology
BLAST of Cp4.1LG20g03110 vs. ExPASy Swiss-Prot
Match: O23034 (Ubiquitin-like modifier-activating enzyme 5 OS=Arabidopsis thaliana OX=3702 GN=At1g05350 PE=3 SV=2)

HSP 1 Score: 636.7 bits (1641), Expect = 1.8e-181
Identity = 337/433 (77.83%), Postives = 376/433 (86.84%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEV  + + +DL+ L++SL+DP+    ++KL+ H+E L  LSK  P RRSKVK++S+EVV
Sbjct: 1   MEVGFKALLDDLDVLEKSLSDPA---LINKLRSHVENLATLSKCNPHRRSKVKELSSEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIV+NYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYD V
Sbjct: 61  DSNPYSRLMALQRMGIVDNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRP+QVGMTKTDAAVQTLA+INPDVVLES+T+NITTVQGFETF SSL NKS
Sbjct: 121 ELANMNRLFFRPDQVGMTKTDAAVQTLAEINPDVVLESFTMNITTVQGFETFTSSLTNKS 180

Query: 181 FSPTKE-GSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGET 240
           F P+KE GSGVDLVLSCVDNYEARMA   ACNEL QTWMESGVSEDAVSGHIQLL+PGET
Sbjct: 181 FCPSKEGGSGVDLVLSCVDNYEARMAVNQACNELRQTWMESGVSEDAVSGHIQLLVPGET 240

Query: 241 ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLG 300
           ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQN+LKFLL FG VSPYLG
Sbjct: 241 ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNSLKFLLNFGEVSPYLG 300

Query: 301 YNSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEAS-TVEEIPLH 360
           YNSLKDFFPTM+MRPNPQCSNV CLERQKEY++AKP RDAAAKA MEA+AS T++E PLH
Sbjct: 301 YNSLKDFFPTMKMRPNPQCSNVACLERQKEYMLAKPERDAAAKAKMEADASTTIDEGPLH 360

Query: 361 ADNEWNISVVDEVEIE--IVGATTSDALPEGLVRELPIADESQHPAPTESAA---PSSDD 420
            DNEWNISVVD+   +     A++SD LPEGL RELP+ADE +      S +      DD
Sbjct: 361 DDNEWNISVVDDENEKDTTKAASSSDTLPEGLTRELPVADEYEKAIAIASGSGETEEEDD 420

Query: 421 LEDLRRQLEALNS 424
           LEDL++QLEALN+
Sbjct: 421 LEDLKKQLEALNA 430

BLAST of Cp4.1LG20g03110 vs. ExPASy Swiss-Prot
Match: Q6K6K7 (Ubiquitin-like modifier-activating enzyme 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0506500 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.6e-167
Identity = 309/427 (72.37%), Postives = 356/427 (83.37%), Query Frame = 0

Query: 2   EVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVVD 61
           E +L  +  DL++LKQ   DP+    +D+++   ER+  +       RSK+KDMS+EVVD
Sbjct: 3   EEQLRALLRDLDALKQR-PDPA---AIDRMR---ERVAGMVTPAAAARSKIKDMSSEVVD 62

Query: 62  SNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVE 121
           SNPYSRLMALQRMGIV+NYERIR++S+AIVGIGGVGSVAAEMLTRCGIGRLLLYDYD VE
Sbjct: 63  SNPYSRLMALQRMGIVDNYERIRDYSIAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTVE 122

Query: 122 LANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSF 181
           LANMNRLFFRP+QVGMTKTDAAVQTL+ INPDV LESY+LNITTV+GFETF+ SL  K+ 
Sbjct: 123 LANMNRLFFRPDQVGMTKTDAAVQTLSGINPDVTLESYSLNITTVKGFETFLGSL--KAR 182

Query: 182 SPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETAC 241
           S     +GVDLVLSCVDNYEARM    ACNEL QTWMESGVSEDAVSGHIQLL+PGETAC
Sbjct: 183 SSDGRNTGVDLVLSCVDNYEARMVVNQACNELGQTWMESGVSEDAVSGHIQLLVPGETAC 242

Query: 242 FACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGYN 301
           FACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQN LK+LLKFG VSPYLGYN
Sbjct: 243 FACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNALKYLLKFGQVSPYLGYN 302

Query: 302 SLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHADN 361
           SLKD+FPTMEM+PNPQCSN  C++RQKEY+ +KPARDAAAKA MEAEAS  +E P+H DN
Sbjct: 303 SLKDYFPTMEMKPNPQCSNPACVQRQKEYMQSKPARDAAAKAKMEAEASAADECPVHLDN 362

Query: 362 EWNISVVDEVE--IEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRR 421
           +WNISVVD+ +     + +T +D+LPEGLVRELP AD  Q P    ++    DDLE+L+R
Sbjct: 363 DWNISVVDDSDTVTPSILSTGADSLPEGLVRELPTADSYQEPVAPVTSGAIDDDLEELQR 420

Query: 422 QLEALNS 424
           QL+ALNS
Sbjct: 423 QLDALNS 420

BLAST of Cp4.1LG20g03110 vs. ExPASy Swiss-Prot
Match: Q8VE47 (Ubiquitin-like modifier-activating enzyme 5 OS=Mus musculus OX=10090 GN=Uba5 PE=1 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 5.6e-122
Identity = 238/402 (59.20%), Postives = 297/402 (73.88%), Query Frame = 0

Query: 25  RGPVDKLQLHL--ERLTNLSKSGPVRRSKVKDMSAEVVDSNPYSRLMALQRMGIVENYER 84
           R  V++L+  L  ER       G   R+++++MS EV+DSNPYSRLMAL+RMGIV +Y++
Sbjct: 9   RQRVEELEQELARERTRRSGGDGHCGRTRIQEMSDEVLDSNPYSRLMALKRMGIVSDYKK 68

Query: 85  IREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNRLFFRPEQVGMTKTDA 144
           IR ++VAIVG+GGVGSV AEMLTRCGIG+LLL+DYDKVELANMNRLFF+P Q G++K  A
Sbjct: 69  IRTYAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPYQAGLSKVHA 128

Query: 145 AVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSFSPTKEGSGVDLVLSCVDNYEA 204
           A  TL +INPDV+ E +  NITTV+ FE FM+ + N      +EG  VDLVLSCVDN+EA
Sbjct: 129 AEHTLRNINPDVLFEVHNYNITTVEHFEHFMNRISNGGL---EEGQPVDLVLSCVDNFEA 188

Query: 205 RMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETACFACAPPLVVASGIDERTLKRE 264
           RMA   ACNEL QTWMESGVSE+AVSGHIQL+IPGE+ACFACAPPLVVAS IDE+TLKRE
Sbjct: 189 RMAINTACNELGQTWMESGVSENAVSGHIQLMIPGESACFACAPPLVVASNIDEKTLKRE 248

Query: 265 GVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGYNSLKDFFPTMEMRPNPQCSNVV 324
           GVCAASLPTTMGVVAG+LVQN LKFLLKFG VS YLGYN+++DFFPTM M+PNPQC +  
Sbjct: 249 GVCAASLPTTMGVVAGILVQNVLKFLLKFGTVSFYLGYNAMQDFFPTMFMKPNPQCDDKN 308

Query: 325 CLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHADNEWNISVVDEVEIEIVGATTS- 384
           C ++Q+EY      + AAA  T EAE     E+ +H DNEW I +V EV  E +  ++  
Sbjct: 309 CRKQQEEY-----KKRAAALPTQEAEPQEEAEV-VHEDNEWGIELVSEVSEEELKNSSGP 368

Query: 385 -DALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQLE 420
              LPEG+     +  +++  A   +   S + LEDL  +++
Sbjct: 369 VPTLPEGITVAYTVPKKTEDSASEVTVEDSGESLEDLMARMK 401

BLAST of Cp4.1LG20g03110 vs. ExPASy Swiss-Prot
Match: Q5M7A4 (Ubiquitin-like modifier-activating enzyme 5 OS=Rattus norvegicus OX=10116 GN=Uba5 PE=1 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 7.3e-122
Identity = 233/395 (58.99%), Postives = 288/395 (72.91%), Query Frame = 0

Query: 32  QLHLERLTNLSKSGPVRRSKVKDMSAEVVDSNPYSRLMALQRMGIVENYERIREFSVAIV 91
           +L  ER   ++  G   R++++ MS EVVDSNPYSRLMAL+RMG+V +YE+IR ++VAIV
Sbjct: 18  ELARERSRRIAGDGHCGRTRIQKMSDEVVDSNPYSRLMALKRMGVVSDYEKIRTYAVAIV 77

Query: 92  GIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNRLFFRPEQVGMTKTDAAVQTLADIN 151
           G+GGVGSV AEMLTRCGIG+LLL+DYDKVELANMNRLFF+P Q GM+K  AA  TL  IN
Sbjct: 78  GVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPYQAGMSKVQAAEHTLRSIN 137

Query: 152 PDVVLESYTLNITTVQGFETFMSSLRNKSFSPTKEGSGVDLVLSCVDNYEARMA---ACN 211
           PDV+ E +  NITTV+ FE FM+ + N      +EG  VDLVLSCVDN+EARMA   ACN
Sbjct: 138 PDVLFEVHNYNITTVEHFEHFMNRISNGGL---EEGQPVDLVLSCVDNFEARMAINTACN 197

Query: 212 ELNQTWMESGVSEDAVSGHIQLLIPGETACFACAPPLVVASGIDERTLKREGVCAASLPT 271
           EL QTWMESGVSE+AVSGHIQL++PGE+ACFACAPPLVVAS IDE+TLKREGVCAASLPT
Sbjct: 198 ELGQTWMESGVSENAVSGHIQLMVPGESACFACAPPLVVASNIDEKTLKREGVCAASLPT 257

Query: 272 TMGVVAGLLVQNTLKFLLKFGHVSPYLGYNSLKDFFPTMEMRPNPQCSNVVCLERQKEYV 331
           TMGVVAG+LVQN LKFLLKFG VS YLGYN+++DFFPTM M+PNPQC +  C ++Q+EY 
Sbjct: 258 TMGVVAGILVQNVLKFLLKFGTVSFYLGYNAMQDFFPTMFMKPNPQCDDKNCRKQQEEYK 317

Query: 332 IAKPARDAAAKATMEAEASTVEEIPLHADNEWNISVVDEVEIEIVGATTS--DALPEGLV 391
              PA+     A  E      EE  +H DNEW I +V EV  E +  ++     LPEG+ 
Sbjct: 318 KRAPAQPTQETAPQE------EEEVVHEDNEWGIELVSEVSEEELKNSSGPVPTLPEGIT 377

Query: 392 RELPIADESQHPAPTESAAPSSDDLEDLRRQLEAL 422
               +  + +      +   S + LEDL  +++ +
Sbjct: 378 VAYTVPKKREDSVSEVTVEDSGESLEDLMARMKKM 403

BLAST of Cp4.1LG20g03110 vs. ExPASy Swiss-Prot
Match: Q3KQ23 (Ubiquitin-like modifier-activating enzyme 5 OS=Xenopus laevis OX=8355 GN=uba5 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.1e-120
Identity = 237/404 (58.66%), Postives = 292/404 (72.28%), Query Frame = 0

Query: 25  RGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVVDSNPYSRLMALQRMGIVENYERIR 84
           R  V +L+  L+R+ N    G   R+K++ MSAEVVDSNPYSRLMAL+RMGIVENYE+IR
Sbjct: 9   RSRVRELEEELDRVRNGQHEG--HRTKIEKMSAEVVDSNPYSRLMALKRMGIVENYEKIR 68

Query: 85  EFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNRLFFRPEQVGMTKTDAAV 144
            F+VA+VG+GGVGSV AEMLTRCGIG+LLL+DYDKVELANMNRLFF+P Q G++K +AA 
Sbjct: 69  TFTVAVVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVEAAE 128

Query: 145 QTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSFSPTKEGSGVDLVLSCVDNYEARM 204
            TL +INPDV  E +  NITT+  F+ FM  +   S    KEGS VDLVLSCVDN+EARM
Sbjct: 129 HTLRNINPDVQFEVHNYNITTLDNFQHFMDRI---SKGGLKEGSPVDLVLSCVDNFEARM 188

Query: 205 A---ACNELNQTWMESGVSEDAVSGHIQLLIPGETACFACAPPLVVASGIDERTLKREGV 264
           A   ACNEL Q WMESGVSE+AVSGHIQL+ PGETACFACAPPLVVA+ IDE+TLKREGV
Sbjct: 189 AINTACNELGQVWMESGVSENAVSGHIQLIKPGETACFACAPPLVVAANIDEKTLKREGV 248

Query: 265 CAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGYNSLKDFFPTMEMRPNPQCSNVVCL 324
           CAASLPTTMGVVAG+LVQN LK+LL FG VS YLGYN+++DFFPTM M+PNPQC +  C 
Sbjct: 249 CAASLPTTMGVVAGILVQNVLKYLLNFGTVSFYLGYNAMQDFFPTMAMKPNPQCDDKYCR 308

Query: 325 ERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHADNEWNISVVDEVEIEIVGATTSDA- 384
           ++Q+E+ + + A+       +E      EE  +H DN+W I +V EV  E + A +    
Sbjct: 309 KQQEEFKLKEAAKPKQETVVVE------EEEVVHEDNDWGIELVSEVSEEELKAASGPVP 368

Query: 385 -LPEGL--VRELPIADESQHPAPTESAAPSSDDLEDLRRQLEAL 422
            LPEG+     +PI      P    +   S   L++L  Q++ L
Sbjct: 369 DLPEGIKVAYTIPIT----KPTSGFTVEDSEQSLDELMAQMKNL 397

BLAST of Cp4.1LG20g03110 vs. NCBI nr
Match: XP_023520116.1 (ubiquitin-like modifier-activating enzyme 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 822 bits (2122), Expect = 8.03e-300
Identity = 423/426 (99.30%), Postives = 423/426 (99.30%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV
Sbjct: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. NCBI nr
Match: XP_022924151.1 (ubiquitin-like modifier-activating enzyme 5 [Cucurbita moschata])

HSP 1 Score: 812 bits (2098), Expect = 3.65e-296
Identity = 419/426 (98.36%), Postives = 419/426 (98.36%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMW DLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV
Sbjct: 1   MEVELEEMWKDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKA MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEI GATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIEGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. NCBI nr
Match: KAG7019743.1 (Ubiquitin-like modifier-activating enzyme 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 811 bits (2094), Expect = 1.49e-295
Identity = 418/426 (98.12%), Postives = 419/426 (98.36%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMW DLESLKQSLTDPSH+GPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV
Sbjct: 1   MEVELEEMWKDLESLKQSLTDPSHQGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKA MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEI GATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIEGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. NCBI nr
Match: XP_023001092.1 (ubiquitin-like modifier-activating enzyme 5 [Cucurbita maxima])

HSP 1 Score: 810 bits (2093), Expect = 2.11e-295
Identity = 418/426 (98.12%), Postives = 419/426 (98.36%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRS+VKDMSAEVV
Sbjct: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSRVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFET MSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETCMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKA MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEI GATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIEGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. NCBI nr
Match: KAG6584152.1 (Ubiquitin-like modifier-activating enzyme 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808 bits (2086), Expect = 2.46e-294
Identity = 417/426 (97.89%), Postives = 418/426 (98.12%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMW DLESLKQSLTDPSH+GPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV
Sbjct: 1   MEVELEEMWKDLESLKQSLTDPSHQGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIG LLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGCLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKA MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEI GATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIEGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. ExPASy TrEMBL
Match: A0A6J1E8S4 (Ubiquitin-like modifier-activating enzyme 5 OS=Cucurbita moschata OX=3662 GN=LOC111431678 PE=3 SV=1)

HSP 1 Score: 812 bits (2098), Expect = 1.77e-296
Identity = 419/426 (98.36%), Postives = 419/426 (98.36%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMW DLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV
Sbjct: 1   MEVELEEMWKDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKA MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEI GATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIEGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. ExPASy TrEMBL
Match: A0A6J1KFH9 (Ubiquitin-like modifier-activating enzyme 5 OS=Cucurbita maxima OX=3661 GN=LOC111495339 PE=3 SV=1)

HSP 1 Score: 810 bits (2093), Expect = 1.02e-295
Identity = 418/426 (98.12%), Postives = 419/426 (98.36%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRS+VKDMSAEVV
Sbjct: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSRVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFET MSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETCMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKA MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIEI GATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIEIEGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. ExPASy TrEMBL
Match: A0A0A0LR68 (Ubiquitin-like modifier-activating enzyme 5 OS=Cucumis sativus OX=3659 GN=Csa_1G050490 PE=3 SV=1)

HSP 1 Score: 778 bits (2009), Expect = 6.46e-283
Identity = 400/426 (93.90%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           ME EL++MWNDLESLK SLTDPS RGPVDKLQLH+ERLTNLSKS PVRRSKVKDMSAEVV
Sbjct: 1   MEAELKDMWNDLESLKHSLTDPSLRGPVDKLQLHVERLTNLSKSVPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYT NITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTFNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKD+FPTMEMRPNPQCSNV CLERQKE++IAKPARDAAA A MEAEA TVEEIPLHAD
Sbjct: 301 NSLKDYFPTMEMRPNPQCSNVACLERQKEFIIAKPARDAAAMAKMEAEALTVEEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIE VGAT+SDALPEGLVRELP ADESQHP P E AAPS DDLEDL+RQ
Sbjct: 361 NEWNISVVDEVEIETVGATSSDALPEGLVRELPNADESQHPPPAEPAAPSLDDLEDLKRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. ExPASy TrEMBL
Match: A0A5D3BJG6 (Ubiquitin-like modifier-activating enzyme 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004690 PE=3 SV=1)

HSP 1 Score: 777 bits (2007), Expect = 1.30e-282
Identity = 399/426 (93.66%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           ME EL++MWNDLES+KQSLTDPS RGPVDKLQLH+ERLTNLSKS PVRRSKVKDMSAEVV
Sbjct: 1   MEAELKDMWNDLESMKQSLTDPSLRGPVDKLQLHVERLTNLSKSVPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRP+QVGMTKTDAAVQTL DINPDVVLESYTLNITTVQGFETFMSSLRNKS
Sbjct: 121 ELANMNRLFFRPDQVGMTKTDAAVQTLEDINPDVVLESYTLNITTVQGFETFMSSLRNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNEL QTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELKQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKD+FPTMEMRPNPQCSNV CLERQKE++IAKPARDAAAKA MEAEA TVEE PLHAD
Sbjct: 301 NSLKDYFPTMEMRPNPQCSNVACLERQKEFIIAKPARDAAAKAKMEAEALTVEEFPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDEVEIE VGAT+SDALPEGLVRELP ADESQHP P E AAPS DDLEDLRRQ
Sbjct: 361 NEWNISVVDEVEIETVGATSSDALPEGLVRELPNADESQHPPPVEPAAPSLDDLEDLRRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. ExPASy TrEMBL
Match: A0A6J1C7T0 (Ubiquitin-like modifier-activating enzyme 5 OS=Momordica charantia OX=3673 GN=LOC111008772 PE=3 SV=1)

HSP 1 Score: 776 bits (2004), Expect = 3.73e-282
Identity = 399/426 (93.66%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEVEL+EMWNDLESLKQSLTDP+ RGPVDKLQLHLERLTNLSK+GPVRRSKVKDMSAEVV
Sbjct: 1   MEVELKEMWNDLESLKQSLTDPALRGPVDKLQLHLERLTNLSKAGPVRRSKVKDMSAEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIVENYERIR FSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV
Sbjct: 61  DSNPYSRLMALQRMGIVENYERIRGFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRPEQVGMTKTDAAVQTL DINPDVVLESYTLNITTVQGFETFMSSL+NKS
Sbjct: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLEDINPDVVLESYTLNITTVQGFETFMSSLKNKS 180

Query: 181 FSPTKEGSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240
           FSPTKEGSGVDLVLSCVDNYEARMA   ACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181 FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300
           CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY
Sbjct: 241 CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 301 NSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEASTVEEIPLHAD 360
           NSLKD+FPTMEMRPNPQCSNV CLERQKEY+IAKPARDAAAKA MEAE  TV EIPLHAD
Sbjct: 301 NSLKDYFPTMEMRPNPQCSNVACLERQKEYIIAKPARDAAAKAKMEAEKLTVVEIPLHAD 360

Query: 361 NEWNISVVDEVEIEIVGATTSDALPEGLVRELPIADESQHPAPTESAAPSSDDLEDLRRQ 420
           NEWNISVVDE+E E VGAT+SDALPEGLVRELP ADES HP P ESAAPS DDLEDL+RQ
Sbjct: 361 NEWNISVVDELETENVGATSSDALPEGLVRELPNADESHHPPPAESAAPSIDDLEDLKRQ 420

Query: 421 LEALNS 423
           LEALNS
Sbjct: 421 LEALNS 426

BLAST of Cp4.1LG20g03110 vs. TAIR 10
Match: AT1G05350.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 636.7 bits (1641), Expect = 1.3e-182
Identity = 337/433 (77.83%), Postives = 376/433 (86.84%), Query Frame = 0

Query: 1   MEVELEEMWNDLESLKQSLTDPSHRGPVDKLQLHLERLTNLSKSGPVRRSKVKDMSAEVV 60
           MEV  + + +DL+ L++SL+DP+    ++KL+ H+E L  LSK  P RRSKVK++S+EVV
Sbjct: 1   MEVGFKALLDDLDVLEKSLSDPA---LINKLRSHVENLATLSKCNPHRRSKVKELSSEVV 60

Query: 61  DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120
           DSNPYSRLMALQRMGIV+NYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYD V
Sbjct: 61  DSNPYSRLMALQRMGIVDNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTV 120

Query: 121 ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKS 180
           ELANMNRLFFRP+QVGMTKTDAAVQTLA+INPDVVLES+T+NITTVQGFETF SSL NKS
Sbjct: 121 ELANMNRLFFRPDQVGMTKTDAAVQTLAEINPDVVLESFTMNITTVQGFETFTSSLTNKS 180

Query: 181 FSPTKE-GSGVDLVLSCVDNYEARMA---ACNELNQTWMESGVSEDAVSGHIQLLIPGET 240
           F P+KE GSGVDLVLSCVDNYEARMA   ACNEL QTWMESGVSEDAVSGHIQLL+PGET
Sbjct: 181 FCPSKEGGSGVDLVLSCVDNYEARMAVNQACNELRQTWMESGVSEDAVSGHIQLLVPGET 240

Query: 241 ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLG 300
           ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQN+LKFLL FG VSPYLG
Sbjct: 241 ACFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNSLKFLLNFGEVSPYLG 300

Query: 301 YNSLKDFFPTMEMRPNPQCSNVVCLERQKEYVIAKPARDAAAKATMEAEAS-TVEEIPLH 360
           YNSLKDFFPTM+MRPNPQCSNV CLERQKEY++AKP RDAAAKA MEA+AS T++E PLH
Sbjct: 301 YNSLKDFFPTMKMRPNPQCSNVACLERQKEYMLAKPERDAAAKAKMEADASTTIDEGPLH 360

Query: 361 ADNEWNISVVDEVEIE--IVGATTSDALPEGLVRELPIADESQHPAPTESAA---PSSDD 420
            DNEWNISVVD+   +     A++SD LPEGL RELP+ADE +      S +      DD
Sbjct: 361 DDNEWNISVVDDENEKDTTKAASSSDTLPEGLTRELPVADEYEKAIAIASGSGETEEEDD 420

Query: 421 LEDLRRQLEALNS 424
           LEDL++QLEALN+
Sbjct: 421 LEDLKKQLEALNA 430

BLAST of Cp4.1LG20g03110 vs. TAIR 10
Match: AT5G37530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-09
Identity = 41/135 (30.37%), Postives = 67/135 (49.63%), Query Frame = 0

Query: 77  VENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNR-LFFRPEQV 136
           +E+  ++    V ++G+GGVGS AA ML R G+G+LLL D+D+V L+++NR        V
Sbjct: 85  LESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSLNRHAVATRADV 144

Query: 137 GMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSFSPTKEGSGVDLVLS 196
           G+ K     +  + I P+  +E+  +              L + S          D VL 
Sbjct: 145 GIPKAMCLKKHFSSIFPECHIEAKVM--------------LYDSSSEEEILSGKPDFVLD 204

Query: 197 CVDNYEAR---MAAC 208
           C+DN + +   +AAC
Sbjct: 205 CIDNIDTKVGLLAAC 205

BLAST of Cp4.1LG20g03110 vs. TAIR 10
Match: AT5G37530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-09
Identity = 41/135 (30.37%), Postives = 67/135 (49.63%), Query Frame = 0

Query: 77  VENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNR-LFFRPEQV 136
           +E+  ++    V ++G+GGVGS AA ML R G+G+LLL D+D+V L+++NR        V
Sbjct: 84  LESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSLNRHAVATRADV 143

Query: 137 GMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSFSPTKEGSGVDLVLS 196
           G+ K     +  + I P+  +E+  +              L + S          D VL 
Sbjct: 144 GIPKAMCLKKHFSSIFPECHIEAKVM--------------LYDSSSEEEILSGKPDFVLD 203

Query: 197 CVDNYEAR---MAAC 208
           C+DN + +   +AAC
Sbjct: 204 CIDNIDTKVGLLAAC 204

BLAST of Cp4.1LG20g03110 vs. TAIR 10
Match: AT5G55130.1 (co-factor for nitrate, reductase and xanthine dehydrogenase 5 )

HSP 1 Score: 60.1 bits (144), Expect = 5.0e-09
Identity = 49/183 (26.78%), Postives = 78/183 (42.62%), Query Frame = 0

Query: 65  YSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELAN 124
           YSR + L     VE    + + SV ++G GG+GS A   L  CG+G+L + D+D VEL N
Sbjct: 72  YSRQLLLPSFA-VEGQSNLLKSSVLVIGAGGLGSPALLYLAACGVGQLGIIDHDVVELNN 131

Query: 125 MNRLFFRPEQ-VGMTKTDAAVQTLADINPDVVLESYTLNITTVQGFETFMSSLRNKSFSP 184
           M+R     E  +G  K  +A      IN  + ++ Y   + T    E             
Sbjct: 132 MHRQIIHTEAFIGHPKVKSAAAACRSINSTIKVDEYVEALRTSNALEIL----------- 191

Query: 185 TKEGSGVDLVLSCVDNYEAR--MAACNELNQTWMESGVSEDAVSGHIQLLIPGETACFAC 244
               S  D+++   DN  +R  ++ C  L    + SG +   + G + +       C+ C
Sbjct: 192 ----SQYDIIVDATDNPPSRYMISDCCVLLGKPLVSGAAL-GMEGQLTVYNHNGGPCYRC 237

BLAST of Cp4.1LG20g03110 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 49.7 bits (117), Expect = 6.8e-06
Identity = 45/174 (25.86%), Postives = 79/174 (45.40%), Query Frame = 0

Query: 83  IREF-SVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKVELANMNRLF-FRPEQVGMTKT 142
           IR++  + ++G GG+G    + L   G   L + D D++E+ N+NR F FR E VG  K 
Sbjct: 43  IRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKA 102

Query: 143 DAAVQTLAD--INPDVVLESYTLNITTVQGFETF-MSSLRNKSFSPTKEGSGVDLVLSC- 202
           + A + + +     ++V     +    ++ +  F + +L   S    K  +GV    +C 
Sbjct: 103 EVAAKRVMERVSGVEIVPHFSRIEDKEIEFYNDFNIIALGLDSIEARKYINGV----ACG 162

Query: 203 VDNYEARMAACNELNQTWMESGVSEDAVSGHIQLLIPGETACFACA----PPLV 247
              Y        E  +  ++ G   +   GH ++++PG T CF C     PP V
Sbjct: 163 FLEYNEDDTPKRETIKPMVDGGT--EGFKGHARVILPGVTPCFECTIYLFPPQV 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O230341.8e-18177.83Ubiquitin-like modifier-activating enzyme 5 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q6K6K72.6e-16772.37Ubiquitin-like modifier-activating enzyme 5 OS=Oryza sativa subsp. japonica OX=3... [more]
Q8VE475.6e-12259.20Ubiquitin-like modifier-activating enzyme 5 OS=Mus musculus OX=10090 GN=Uba5 PE=... [more]
Q5M7A47.3e-12258.99Ubiquitin-like modifier-activating enzyme 5 OS=Rattus norvegicus OX=10116 GN=Uba... [more]
Q3KQ231.1e-12058.66Ubiquitin-like modifier-activating enzyme 5 OS=Xenopus laevis OX=8355 GN=uba5 PE... [more]
Match NameE-valueIdentityDescription
XP_023520116.18.03e-30099.30ubiquitin-like modifier-activating enzyme 5 [Cucurbita pepo subsp. pepo][more]
XP_022924151.13.65e-29698.36ubiquitin-like modifier-activating enzyme 5 [Cucurbita moschata][more]
KAG7019743.11.49e-29598.12Ubiquitin-like modifier-activating enzyme 5 [Cucurbita argyrosperma subsp. argyr... [more]
XP_023001092.12.11e-29598.12ubiquitin-like modifier-activating enzyme 5 [Cucurbita maxima][more]
KAG6584152.12.46e-29497.89Ubiquitin-like modifier-activating enzyme 5, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
A0A6J1E8S41.77e-29698.36Ubiquitin-like modifier-activating enzyme 5 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KFH91.02e-29598.12Ubiquitin-like modifier-activating enzyme 5 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0LR686.46e-28393.90Ubiquitin-like modifier-activating enzyme 5 OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A5D3BJG61.30e-28293.66Ubiquitin-like modifier-activating enzyme 5 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1C7T03.73e-28293.66Ubiquitin-like modifier-activating enzyme 5 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G05350.11.3e-18277.83NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G37530.11.3e-0930.37NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G37530.21.3e-0930.37NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G55130.15.0e-0926.78co-factor for nitrate, reductase and xanthine dehydrogenase 5 [more]
AT5G19180.16.8e-0625.86E1 C-terminal related 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 411..423
NoneNo IPR availableGENE3D3.40.50.720coord: 82..341
e-value: 1.4E-69
score: 236.3
NoneNo IPR availablePIRSRPIRSR039133-2PIRSR039133-2coord: 67..243
e-value: 8.1E-15
score: 52.2
NoneNo IPR availablePIRSRPIRSR039133-3PIRSR039133-3coord: 67..243
e-value: 8.1E-15
score: 52.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 386..423
NoneNo IPR availablePANTHERPTHR10953UBIQUITIN-ACTIVATING ENZYME E1coord: 1..422
NoneNo IPR availablePANTHERPTHR10953:SF212BNAANNG16230D PROTEINcoord: 1..422
NoneNo IPR availableCDDcd00757ThiF_MoeB_HesA_familycoord: 64..309
e-value: 3.57852E-76
score: 234.679
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 65..316
e-value: 8.3E-46
score: 156.3
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 88..116
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 60..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g03110.1Cp4.1LG20g03110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071569 protein ufmylation
cellular_component GO:0005829 cytosol
molecular_function GO:0046872 metal ion binding
molecular_function GO:0071566 UFM1 activating enzyme activity
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity