Homology
BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match:
Q93W57 (11-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 SV=1)
HSP 1 Score: 373.2 bits (957), Expect = 5.8e-102
Identity = 185/335 (55.22%), Postives = 238/335 (71.04%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M+++HTFLNL+APPFTF L LPP+Q FK FLS++G LF+E+V GKVV+ITGASSGIG
Sbjct: 1 MDLIHTFLNLIAPPFTFFFLLFFLPPFQIFKFFLSILGTLFSEDVAGKVVVITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E LAYEYAKRGACLVL ARRE L+EVA+ AR GSPDV+ +RADVSK EDCR+V+++TM
Sbjct: 61 ESLAYEYAKRGACLVLAARRERSLQEVAERARDLGSPDVVVVRADVSKAEDCRKVVDQTM 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
N FGRLDHLVNNA I + M E++ DI +RG+IV
Sbjct: 121 NRFGRLDHLVNNAGIMSVSMLEEVEDITGYRETMDINFWGYVYMTRFAAPYLRNSRGRIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
LS+ +WMP PRMS YN+SKAAI FETLRVE P+IG+T+VTPGF+ESELT+GK N
Sbjct: 181 VLSSSSSWMPTPRMSFYNASKAAISQFFETLRVEFGPDIGITLVTPGFIESELTQGKFYN 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
A G+ + QDMRD + P+ E+ A+++V+ RG+RYVTEP+W+ V Y+WK FCPE
Sbjct: 241 A-GERVIDQDMRDVQVSTTPILRVESAARSIVRSAIRGERYVTEPAWFRVTYWWKLFCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANIL 310
++EW +R++ + + G E + K+ LDYTG L
Sbjct: 301 VMEWVFRLMYLASPGEPEKETFGKKVLDYTGVKSL 334
BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match:
A7LB60 (11-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1 SV=1)
HSP 1 Score: 358.2 bits (918), Expect = 1.9e-97
Identity = 181/335 (54.03%), Postives = 240/335 (71.64%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M+++HTFLNLVAPPFTF L L LPPY K +S++ +L +ENV GKVV ITGASSGIG
Sbjct: 1 MDLIHTFLNLVAPPFTFFFLCLFLPPYWGLKFMVSILSWLLSENVAGKVVHITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E+LAYEYAKRGACL L ARRE L +VAD AR+ GSPDVI +RADVSK EDC R++++T+
Sbjct: 61 EYLAYEYAKRGACLALSARRETALHQVADTARHLGSPDVIVMRADVSKPEDCMRLIDQTV 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
NHFGRLDHLVNNAAI+ +FE+ DI+ +RGKIV
Sbjct: 121 NHFGRLDHLVNNAAISIATLFEETPDISNLRPIMETNFWGSVYTTRYALQHLRKSRGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
+S+ +W+PAPR IY++SKAA+ +++ETLRVE+ EIG+TIVTPG++ESE+TKGK L+
Sbjct: 181 VMSSVDSWLPAPRRHIYSASKAALVSLYETLRVEVGSEIGITIVTPGYIESEITKGKFLS 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
AQG++++ QD+RD + VPV + CA++++K RG R +T PSW+ + Y K CPE
Sbjct: 241 AQGEVDVDQDLRDVEVSAVPVGSVSGCAESIIKSTLRGDRCLTVPSWFRMTYLIKLLCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANIL 310
++EW +R+L + A G SDAL+K+ LD TGA L
Sbjct: 301 LLEWTFRLLYLTAPGTPTSDALSKKILDATGAKNL 335
BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match:
P0DKC5 (11-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)
HSP 1 Score: 355.9 bits (912), Expect = 9.5e-97
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M +++ FLNL AP FTF L LPP+ FK S+ +F+EN+ GKVVLITGASSGIG
Sbjct: 1 MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E LAYEYA RGACL L ARR+N LEEVA+IAR GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61 EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
HFGRLDHLVNNA +T + MFE+I DI + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
A+S+ AW+ APRMS YN+SKAA+ + FET+R+EL ++ +TIVTPG++ESELT+GK +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
+G++ + QDMRD +G PV +A CAK++V G+CR QRYVTEPSW+ V Y WK CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
++EW R+L M G SE AL K+ +D G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331
BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match:
P0DKC6 (11-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)
HSP 1 Score: 355.9 bits (912), Expect = 9.5e-97
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M +++ FLNL AP FTF L LPP+ FK S+ +F+EN+ GKVVLITGASSGIG
Sbjct: 1 MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E LAYEYA RGACL L ARR+N LEEVA+IAR GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61 EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
HFGRLDHLVNNA +T + MFE+I DI + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
A+S+ AW+ APRMS YN+SKAA+ + FET+R+EL ++ +TIVTPG++ESELT+GK +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
+G++ + QDMRD +G PV +A CAK++V G+CR QRYVTEPSW+ V Y WK CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
++EW R+L M G SE AL K+ +D G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331
BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match:
Q9LUE4 (11-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=HSD6 PE=2 SV=1)
HSP 1 Score: 292.4 bits (747), Expect = 1.3e-77
Identity = 149/299 (49.83%), Postives = 200/299 (66.89%), Query Frame = 0
Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
I+ + ++ A YP Y K I R++ +E+VAGKVV+ITGA+SGIGE LAYE
Sbjct: 7 IINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIGEALAYE 66
Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
Y KRGAYLALV R L VA +AE+ GSP L + ADVS L DC+ RL
Sbjct: 67 YGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATVLHFGRL 126
Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
DHLV NAG+ + F + D + MD +FWG+VYCT++A PYLK+ RG+I+ IAS
Sbjct: 127 DHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIVVIASGC 186
Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
++ +PRLSFY ASKAAVI+FYETLR E GS+IG+T+V PG+++SEM++GKFM +DGK+
Sbjct: 187 GYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMTKDGKLV 246
Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 596
+D+ELRD MSV+P+E +A++R+ CRGD Y+ EP W+ K+F E EW+
Sbjct: 247 VDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSEATEWV 305
BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match:
KAG7019754.1 (11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1167 bits (3020), Expect = 0.0
Identity = 601/670 (89.70%), Postives = 605/670 (90.30%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG
Sbjct: 47 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 106
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM
Sbjct: 107 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 166
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIAA--------------------------RGKIV 180
NHFGRLDHLVNNAAITHLVMFEDIADIAA RGKIV
Sbjct: 167 NHFGRLDHLVNNAAITHLVMFEDIADIAAFRQIMDINYWGAVYLTHMAIPYLRYSRGKIV 226
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN
Sbjct: 227 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 286
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE
Sbjct: 287 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 346
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFI 360
IVEWCYRILAMPAAGASESDALTKQALDYTGA V IDRIS+T FPFIYPIY FKFI
Sbjct: 347 IVEWCYRILAMPAAGASESDALTKQALDYTGAKY--VTIDRISETVFPFIYPIYLLFKFI 406
Query: 361 SFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEI 420
SFIIRS SAEDVAGKVVLITGASSGIGEHLAYEYAK GAYLALVARRDHRLR VADVAEI
Sbjct: 407 SFIIRSTSAEDVAGKVVLITGASSGIGEHLAYEYAKTGAYLALVARRDHRLRHVADVAEI 466
Query: 421 LGSPYALVIHADVSNLDDCQRLDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYC 480
LGSPYALVIHADVSNL+DC+R VD DTDFWGTVYC
Sbjct: 467 LGSPYALVIHADVSNLEDCKRC------------------VDTT------DTDFWGTVYC 526
Query: 481 TYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVV 540
TYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVV
Sbjct: 527 TYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVV 586
Query: 541 TPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTE 600
TPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTE
Sbjct: 587 TPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTE 646
Query: 601 PPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPEL 644
PPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPEL
Sbjct: 647 PPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPEL 690
BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match:
KAE8652630.1 (hypothetical protein Csa_013319 [Cucumis sativus])
HSP 1 Score: 724 bits (1870), Expect = 5.42e-254
Identity = 375/678 (55.31%), Postives = 485/678 (71.53%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M++++ FLNL+ + + + P Y FK L I F E+V GKVVLITGASSGIG
Sbjct: 1 MDLINNFLNLIIDRISQTAFSFVFPLYFFFKLLLFPIRSSFAEDVAGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
EH+AYEYAKRGA L LVARRE+ L EVA++A GSP + I ADVS +DC+R + T+
Sbjct: 61 EHVAYEYAKRGAYLALVARREHRLREVAEVAEILGSPFALLIHADVSNVDDCKRCVQTTL 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIAD---IAA-----------------------RGKIV 180
HFGRLDHLVNNA IT + +FE+ D +A+ RGKI+
Sbjct: 121 THFGRLDHLVNNAGITSINLFEEYDDPRNVASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
+++ AW+P PR+S Y++SKAA+ + +ETLRVE+ +IG+TIVTPG ESE+T+GK +
Sbjct: 181 GIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGRDIGITIVTPGLTESEMTQGKFMF 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
GKM L Q++RDA++ ++P+E + AKA+V G CRG YVTEP W + +YWK F PE
Sbjct: 241 EDGKMYLDQELRDAVMSVMPIEAVGSAAKAIVNGGCRGDEYVTEPPWIRMTFYWKMFLPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYP--IYFFFK 360
+VEW + M G+S +++ K+ L++TG LK PFIYP +
Sbjct: 301 MVEWLNYLFIM--TGSSPTESFGKRVLEFTG---LK-----------PFIYPPSLRDGGD 360
Query: 361 FISFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVA 420
+ I +S+ +EDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARR++RLR+VA VA
Sbjct: 361 IVGSIRKSVFSEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRENRLREVAAVA 420
Query: 421 EILGSPYALVIHADVSNLDDCQR-----------LDHLVNNAGITSINLFEEYVDPRNIA 480
+ LGSPYALVI AD+S ++DC+R LDHLVNNAG++S+NLFEEY + +N
Sbjct: 421 QYLGSPYALVIPADISKIEDCKRCIQTTVTHFRRLDHLVNNAGVSSVNLFEEYDNLQNAV 480
Query: 481 SVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETL 540
VMD +FWG VYC+YY IP+LKQ+RGKIIGIASSAAWLP PRLSFY++SKAAVISFYETL
Sbjct: 481 PVMDVNFWGMVYCSYYGIPHLKQSRGKIIGIASSAAWLPAPRLSFYTSSKAAVISFYETL 540
Query: 541 RVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAM 600
RVE+G +IGIT+VTPGL+ESEMT+GKFM +DG +Y+DQ+LRDA +S MPI P+ AV+ +
Sbjct: 541 RVEVGRDIGITIVTPGLVESEMTQGKFMSKDGHLYLDQQLRDATVSAMPIMPIDDAVKGI 600
Query: 601 LRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWLNYLFIMTGSS--PTDTFGKWLLELTG 637
LR+ C+GD Y TEP W++M+FY+K PE+VEW NYL M GSS PTDTFGK L++L+G
Sbjct: 601 LRSVCKGDRYATEPRWMKMAFYYKTLWPELVEWFNYLINMRGSSNSPTDTFGKRLVQLSG 660
BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match:
KAG7012327.1 (11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 701 bits (1810), Expect = 1.52e-246
Identity = 367/645 (56.90%), Postives = 448/645 (69.46%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M ++ +FLNLVAPPFT +SL LLLP Y FK F SL+ LF+E+V GKVVLITGASSGIG
Sbjct: 1 MELIQSFLNLVAPPFTVVSLGLLLPHYVVFKYFFSLVRGLFSEDVTGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E+LAYEYAKRGA L LVARRE+ LEE A+ ARYYGSP+VITI DVS+ DCRR++++TM
Sbjct: 61 ENLAYEYAKRGAQLALVARRESSLEEAAEAARYYGSPEVITIAGDVSELSDCRRIIDDTM 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIAARGKIVAL----SAPPAWMPAPRMS-----IY 180
NHFGRLDHLVNNA I ++ +FE+I DI + KI+ S W+ P + I
Sbjct: 121 NHFGRLDHLVNNAGIANMTLFEEIIDITSFKKIMETNYWGSVYMTWLAIPYLKNRGGKII 180
Query: 181 NSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALNAQGKMELRQDMRDALIG 240
+SKAA+++ FETLRVE++PEIG+T+VTPGFVESE+TKGKAL + G+ME+ QD+RDAL+G
Sbjct: 181 VASKAALRSFFETLRVEMSPEIGITVVTPGFVESEITKGKALYSHGRMEVHQDVRDALVG 240
Query: 241 LVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPEIVEWCYRILAMPAAGAS 300
+PVETA ACAKA+V+ C+G RY+TEPSWY+ YY KAFCPE+VEWCYRIL G S
Sbjct: 241 AIPVETAAACAKAIVRSACQGDRYLTEPSWYNAAYYLKAFCPEVVEWCYRILVYTTPGTS 300
Query: 301 ESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVV 360
++AL KQ LD TGA +TA +YP
Sbjct: 301 AAEALNKQILDLTGA-----------KTA---MYP------------------------- 360
Query: 361 LITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLD 420
I H+AYEYAKRGAYLALVARR++RLR+VA VA GSP+AL
Sbjct: 361 ------PSIHSHMAYEYAKRGAYLALVARRENRLREVAGVARFFGSPFAL---------- 420
Query: 421 DCQRLDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIG 480
D +FWG VYCTY+AIP+LKQ+RGKIIG
Sbjct: 421 ---------------------------------DVNFWGMVYCTYFAIPHLKQSRGKIIG 480
Query: 481 IASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFE 540
I+SSAAWLP PRLSFYSASKAAVISFYETLRVE+G +IGIT+VTPGL+ESEMT+GKFM +
Sbjct: 481 ISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFMSK 540
Query: 541 DGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEV 600
DG +Y+DQELRDA++S MPI + AV ++R+ CRGD Y TEP W+RM+FY+K PE+
Sbjct: 541 DGGLYLDQELRDAMVSAMPIMAIDSAVNGIVRSACRGDRYATEPRWMRMAFYYKTLWPEL 557
Query: 601 VEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 636
VEW+NYL MT SSPTD+FGK LLELTGLK YP SVRS +L +
Sbjct: 601 VEWVNYLIGMTSSSPTDSFGKRLLELTGLKTWFYPESVRSQKLWL 557
BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match:
XP_023519994.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 675 bits (1741), Expect = 2.60e-239
Identity = 337/349 (96.56%), Postives = 338/349 (96.85%), Query Frame = 0
Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
+ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6 SILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 65
Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR
Sbjct: 66 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQRCVDTTVGHFGQ 125
Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 185
Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245
Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL
Sbjct: 246 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 305
Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 644
NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 354
BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match:
XP_022923773.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata])
HSP 1 Score: 656 bits (1693), Expect = 7.41e-232
Identity = 326/349 (93.41%), Postives = 331/349 (94.84%), Query Frame = 0
Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
+ILKV IDRISQT FPFIYPIY FKFISFIIRS SAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6 SILKVTIDRISQTVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIGEHLAY 65
Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNL+DC+R
Sbjct: 66 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLEDCKRCVDTTVSHFGQ 125
Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
LDHLVNNAGITSINLFEEY DPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 185
Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245
Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL
Sbjct: 246 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 305
Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 644
NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQ+G YPN+
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQVGAYPNM 354
BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match:
A0A6J1EAJ7 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111431382 PE=3 SV=1)
HSP 1 Score: 656 bits (1693), Expect = 3.59e-232
Identity = 326/349 (93.41%), Postives = 331/349 (94.84%), Query Frame = 0
Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
+ILKV IDRISQT FPFIYPIY FKFISFIIRS SAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6 SILKVTIDRISQTVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIGEHLAY 65
Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNL+DC+R
Sbjct: 66 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLEDCKRCVDTTVSHFGQ 125
Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
LDHLVNNAGITSINLFEEY DPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 185
Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245
Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL
Sbjct: 246 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 305
Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 644
NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQ+G YPN+
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQVGAYPNM 354
BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match:
A0A6J1KHB1 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111495256 PE=3 SV=1)
HSP 1 Score: 643 bits (1659), Expect = 2.89e-227
Identity = 319/343 (93.00%), Postives = 327/343 (95.34%), Query Frame = 0
Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
N+LK+IIDRISQTAFPFIYPIY FKFI FIIRS SAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6 NLLKIIIDRISQTAFPFIYPIYLLFKFIFFIIRSTSAEDVAGKVVLITGASSGIGEHLAY 65
Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
EYAKRGAYLALVARRDHRLRDVADVAEILGSPYAL+IHADVSNL+DC+R
Sbjct: 66 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALLIHADVSNLEDCKRCVDTTVSHFGR 125
Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
LDHLVNNAGITSINLFEEY DPRNIASVMDTDFWGTVYCTY+AIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYFAIPYLKQTRGKIIGIASS 185
Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245
Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
YIDQELRDAV+SVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEW+
Sbjct: 246 YIDQELRDAVISVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWM 305
Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQI 638
NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQI
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQI 348
BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match:
A0A2U1MAX7 (Hydroxysteroid dehydrogenase 6 OS=Artemisia annua OX=35608 GN=CTI12_AA403160 PE=4 SV=1)
HSP 1 Score: 620 bits (1598), Expect = 1.57e-211
Identity = 339/760 (44.61%), Postives = 458/760 (60.26%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M+ + + LNLVAP T +LF P Y FK + +F E+V GKVVLI GASSGIG
Sbjct: 1 MDFVDSVLNLVAPIATICALFCFTPLYIGFKLLWFIFRSIFKEDVAGKVVLIVGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
EH+AYEY+KRGACLVL ARR++ L+EVAD A G+PD I IRADVS EDC+R++N+T+
Sbjct: 61 EHVAYEYSKRGACLVLAARRKHSLQEVADTAVSLGAPDAIAIRADVSNIEDCKRLINKTI 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
+HFG+LDHLVN A IT + M EDI DI ++GKIV
Sbjct: 121 SHFGKLDHLVNCAGITSMSMLEDITDITRFTQVLDTNFWGYVYMTHLSIPHLRRSKGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
A+++ WMP PRM+IYN+SKAA+ + +E+LR+EL E+G+TIVTPG ESE+++GK ++
Sbjct: 181 AIASSATWMPVPRMNIYNASKAAVVSFYESLRMELGSEVGITIVTPGLTESEMSQGKIMD 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
G+M + QDMRDA + +VP+E A A A+V G RG + P W YW AF PE
Sbjct: 241 KDGEMVIDQDMRDAEMSIVPIELAAKSAVAIVDGASRGDLSLAVPRWVQTTIYWVAFFPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGAN-----------ILK------------- 360
+++W + GAS DA +K LD G ++K
Sbjct: 301 MIDWVNHWFLLAEPGASPLDAPSKMLLDVPGLRHIVQPDSVISPVIKEYDYKLVVHTPHK 360
Query: 361 --------------------------------------------VIIDRISQTAFPFI-- 420
+II ++Q F+
Sbjct: 361 KRSDVVAVLIIPLEMLSRSCVIPSCVPFFVDIALLTDKPDSRHLIIIINLNQPDMDFVDS 420
Query: 421 -----------------YPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 480
P+Y FK + +I RSIS EDVAGKVVLI GASSGIGEH+AYE
Sbjct: 421 VLNLVAPIATICALFCFTPLYIGFKVLWYIFRSISKEDVAGKVVLIVGASSGIGEHVAYE 480
Query: 481 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQRL----------- 540
YAKRGA L L ARR H L++VAD A LG+P A+ I ADVSN++DC+RL
Sbjct: 481 YAKRGACLVLAARRKHSLQEVADTAVSLGAPDAIAIRADVSNIEDCKRLINKTISHFGKL 540
Query: 541 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 600
DHLVN AGITS+++ E+ D VMDT+FWG VY TY +IP+L++++GKI+ IASSA
Sbjct: 541 DHLVNCAGITSMSMLEDITDITRFTQVMDTNFWGYVYMTYLSIPHLRRSKGKIVVIASSA 600
Query: 601 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 634
W+P PR++ Y+ASKAAV+SFYE+LR+E+GSE+GIT+VTPGL ESEM++GK M +DG+M
Sbjct: 601 TWMPVPRMNIYNASKAAVVSFYESLRMELGSEVGITIVTPGLTESEMSQGKIMDKDGEMV 660
BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match:
A0A4Y1RH78 (Hydroxysteroid dehydrogenase 1 OS=Prunus dulcis OX=3755 GN=Prudu_014581 PE=4 SV=1)
HSP 1 Score: 612 bits (1577), Expect = 1.52e-210
Identity = 328/651 (50.38%), Postives = 431/651 (66.21%), Query Frame = 0
Query: 2 NILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIGE 61
N++H FLNLVAPPFTF SL L LPP+ FKSFLS++ +F+ENV+GKVVLITGASSGIGE
Sbjct: 5 NLIHKFLNLVAPPFTFFSLCLFLPPFYLFKSFLSVLSSIFSENVNGKVVLITGASSGIGE 64
Query: 62 HLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETMN 121
HLAYEYAKRGA LVLV RRE+ L EV D A GSPDV+ +RADVSK EDC+R+++ET+N
Sbjct: 65 HLAYEYAKRGARLVLVGRREDSLREVVDRASDRGSPDVLMVRADVSKVEDCKRIVDETIN 124
Query: 122 HFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIVA 181
HFGRLDHLVNNA IT L E+ DI ++GKIV
Sbjct: 125 HFGRLDHLVNNAGITSLGRLEEATDITNFRAIMDTNFWGSVYTTRFAIPHLRCSKGKIVV 184
Query: 182 LSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALNA 241
LS+ +W+P PR SIYN+SKAA+ +M+ETLR+E P I +TIVTPG++ESELT+GK L +
Sbjct: 185 LSSAASWLPIPRQSIYNASKAALLSMYETLRIEFGPNIQITIVTPGYIESELTQGKFLMS 244
Query: 242 Q-GKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 301
G+M + QD+RDA + PV + E CAKA+V +CRG RY+TEP W+ V Y WK FCPE
Sbjct: 245 DDGEMIVDQDLRDAQVSATPVGSVEGCAKAIVNSVCRGDRYLTEPEWFGVTYIWKVFCPE 304
Query: 302 IVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFI 361
++EW YR++ + G+S +A +K+ LDYTGA V+ + +FI
Sbjct: 305 LLEWGYRLMYVTRPGSSAKEAPSKKILDYTGAE--NVLYPETLHSP-----------EFI 364
Query: 362 SFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEI 421
SF+ RS S E++ GKVVLITGASSGIGEHLAYEYAKRGA LAL ARR+ RLR VAD A
Sbjct: 365 SFMRRSKSIENMGGKVVLITGASSGIGEHLAYEYAKRGARLALAARREDRLRAVADKARG 424
Query: 422 LGSPYALVIHADVSNLDDCQRL-----------DHLVNNAGITSINLFEEYVDPR--NIA 481
LGSP A+V+ ADVS L+ C RL DHLVNNAG+ FE++ + +
Sbjct: 425 LGSPDAIVLPADVSILEHCNRLVNETVNHFGQLDHLVNNAGVLQAGSFEDWTQGQFSKLV 484
Query: 482 SVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETL 541
S+MD +FWG+VYCT++++PYL++++GKI+ I+S A+W P+ + F+ETL
Sbjct: 485 SIMDINFWGSVYCTHFSLPYLRKSKGKIVVISSFASWFSAPK---------QLTCFFETL 544
Query: 542 RVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAM 601
R E+GS+IGIT+V+PG+IESEMT Q L ++MP + G +A+
Sbjct: 545 RAELGSDIGITIVSPGVIESEMTGSS-----------QFLSQVRSNLMPSKSTEGCTKAI 604
Query: 602 LRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGK 612
+ RGD Y+TEP W++ F+ ++F PE++E L L + T +P T K
Sbjct: 605 VDRAWRGDMYLTEPAWVKFGFWLRVFCPELLEKLFQL-MATKKNPAGTSIK 621
BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match:
A0A6J1EAX4 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111431503 PE=3 SV=1)
HSP 1 Score: 589 bits (1519), Expect = 5.56e-206
Identity = 305/332 (91.87%), Postives = 305/332 (91.87%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG
Sbjct: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM
Sbjct: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIAA--------------------------RGKIV 180
N FGRLDHLVNNAAITHLVMFEDIADIAA RGKIV
Sbjct: 121 NRFGRLDHLVNNAAITHLVMFEDIADIAAFRQIMDINYWGAVYLTHMAIPYLRYSRGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN
Sbjct: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE
Sbjct: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGA 306
IVEWCYRILAMPAAGASESDALTKQALDYTGA
Sbjct: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGA 332
BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match:
AT5G50600.1 (hydroxysteroid dehydrogenase 1 )
HSP 1 Score: 355.9 bits (912), Expect = 6.8e-98
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M +++ FLNL AP FTF L LPP+ FK S+ +F+EN+ GKVVLITGASSGIG
Sbjct: 1 MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E LAYEYA RGACL L ARR+N LEEVA+IAR GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61 EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
HFGRLDHLVNNA +T + MFE+I DI + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
A+S+ AW+ APRMS YN+SKAA+ + FET+R+EL ++ +TIVTPG++ESELT+GK +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
+G++ + QDMRD +G PV +A CAK++V G+CR QRYVTEPSW+ V Y WK CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
++EW R+L M G SE AL K+ +D G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331
BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match:
AT5G50700.1 (hydroxysteroid dehydrogenase 1 )
HSP 1 Score: 355.9 bits (912), Expect = 6.8e-98
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0
Query: 1 MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
M +++ FLNL AP FTF L LPP+ FK S+ +F+EN+ GKVVLITGASSGIG
Sbjct: 1 MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60
Query: 61 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
E LAYEYA RGACL L ARR+N LEEVA+IAR GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61 EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120
Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
HFGRLDHLVNNA +T + MFE+I DI + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180
Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
A+S+ AW+ APRMS YN+SKAA+ + FET+R+EL ++ +TIVTPG++ESELT+GK +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240
Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
+G++ + QDMRD +G PV +A CAK++V G+CR QRYVTEPSW+ V Y WK CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300
Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
++EW R+L M G SE AL K+ +D G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331
BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match:
AT5G50770.1 (hydroxysteroid dehydrogenase 6 )
HSP 1 Score: 292.4 bits (747), Expect = 9.2e-79
Identity = 149/299 (49.83%), Postives = 200/299 (66.89%), Query Frame = 0
Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
I+ + ++ A YP Y K I R++ +E+VAGKVV+ITGA+SGIGE LAYE
Sbjct: 7 IINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIGEALAYE 66
Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
Y KRGAYLALV R L VA +AE+ GSP L + ADVS L DC+ RL
Sbjct: 67 YGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATVLHFGRL 126
Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
DHLV NAG+ + F + D + MD +FWG+VYCT++A PYLK+ RG+I+ IAS
Sbjct: 127 DHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIVVIASGC 186
Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
++ +PRLSFY ASKAAVI+FYETLR E GS+IG+T+V PG+++SEM++GKFM +DGK+
Sbjct: 187 GYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMTKDGKLV 246
Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 596
+D+ELRD MSV+P+E +A++R+ CRGD Y+ EP W+ K+F E EW+
Sbjct: 247 VDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSEATEWV 305
BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match:
AT5G50590.1 (hydroxysteroid dehydrogenase 4 )
HSP 1 Score: 250.8 bits (639), Expect = 3.1e-66
Identity = 132/296 (44.59%), Postives = 184/296 (62.16%), Query Frame = 0
Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
I +++ ++ + P FFK + FI +E V GKVV+ITG+SSGIGEHLAYE
Sbjct: 7 IFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIGEHLAYE 66
Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
YA+RGAYL LVARR+ RL+ VAD LGSP V+ DVS + DC+ RL
Sbjct: 67 YARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETISRFGRL 126
Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
DHLVNNAGI FE+Y + ++ +++T+FWG VY T++AIP+LK+T+GKII +AS A
Sbjct: 127 DHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGKIIAVASPA 186
Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
W PR+S Y+ASKAA+I+FYETLR+E+ E+G+T+V PGLIE+ T + E
Sbjct: 187 GWSGVPRMSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLAE----- 246
Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVV 593
+ V+ IE +A++ CRG +V EP W+R+ F+ PE++
Sbjct: 247 -----KQDWSQVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPELL 292
BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match:
AT5G50690.1 (hydroxysteroid dehydrogenase 7 )
HSP 1 Score: 250.8 bits (639), Expect = 3.1e-66
Identity = 132/296 (44.59%), Postives = 184/296 (62.16%), Query Frame = 0
Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
I +++ ++ + P FFK + FI +E V GKVV+ITG+SSGIGEHLAYE
Sbjct: 7 IFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIGEHLAYE 66
Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
YA+RGAYL LVARR+ RL+ VAD LGSP V+ DVS + DC+ RL
Sbjct: 67 YARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETISRFGRL 126
Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
DHLVNNAGI FE+Y + ++ +++T+FWG VY T++AIP+LK+T+GKII +AS A
Sbjct: 127 DHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGKIIAVASPA 186
Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
W PR+S Y+ASKAA+I+FYETLR+E+ E+G+T+V PGLIE+ T + E
Sbjct: 187 GWSGVPRMSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLAE----- 246
Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVV 593
+ V+ IE +A++ CRG +V EP W+R+ F+ PE++
Sbjct: 247 -----KQDWSQVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPELL 292
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q93W57 | 5.8e-102 | 55.22 | 11-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 S... | [more] |
A7LB60 | 1.9e-97 | 54.03 | 11-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1... | [more] |
P0DKC5 | 9.5e-97 | 53.47 | 11-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 ... | [more] |
P0DKC6 | 9.5e-97 | 53.47 | 11-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 ... | [more] |
Q9LUE4 | 1.3e-77 | 49.83 | 11-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=H... | [more] |
Match Name | E-value | Identity | Description | |
KAG7019754.1 | 0.0 | 89.70 | 11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. ... | [more] |
KAE8652630.1 | 5.42e-254 | 55.31 | hypothetical protein Csa_013319 [Cucumis sativus] | [more] |
KAG7012327.1 | 1.52e-246 | 56.90 | 11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. ... | [more] |
XP_023519994.1 | 2.60e-239 | 96.56 | 11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022923773.1 | 7.41e-232 | 93.41 | 11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1EAJ7 | 3.59e-232 | 93.41 | 11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A6J1KHB1 | 2.89e-227 | 93.00 | 11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A2U1MAX7 | 1.57e-211 | 44.61 | Hydroxysteroid dehydrogenase 6 OS=Artemisia annua OX=35608 GN=CTI12_AA403160 PE=... | [more] |
A0A4Y1RH78 | 1.52e-210 | 50.38 | Hydroxysteroid dehydrogenase 1 OS=Prunus dulcis OX=3755 GN=Prudu_014581 PE=4 SV=... | [more] |
A0A6J1EAX4 | 5.56e-206 | 91.87 | 11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO... | [more] |