Cp4.1LG20g02990 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g02990
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description11-beta-hydroxysteroid dehydrogenase 1B-like
LocationCp4.1LG20: 1657742 .. 1663218 (-)
RNA-Seq ExpressionCp4.1LG20g02990
SyntenyCp4.1LG20g02990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGAGAATTCTTAGTCCGGCAGCCATGAATATCCTTCACACCTTTCTGAATTTAGTCGCTCCACCCTTTACTTTCATTTCCCTCTTCCTTTTACTACCGCCTTATCAAGCTTTCAAATCATTTCTCTCTTTGATTGGTTTTCTGTTCACGGAAAATGTCGACGGAAAGGTGGTTCTTATCACCGGCGCTTCCTCCGGCATCGGCGAGGTAACCCATCTGAAAATATGGGGGGAGAAGGATAAAATAGGGATTAATGTTTGGTTTTGATTGCAGCATTTGGCGTATGAGTATGCGAAGAGAGGGGCTTGCTTAGTGCTTGTGGCTAGACGAGAGAATCTACTGGAAGAAGTTGCAGACATAGCTCGGTATTATGGATCACCAGATGTAATCACCATTCGAGCTGATGTTTCGAAGTTTGAAGATTGTCGGCGGGTTATGAACGAGACGATGAATCATTTTGGGAGATGTGAGTGCAAATTGTTCTGAAATTAAGTTGAGAAAAGCAAAAACAGAGTCGTTTTTCTAATAATGATTTGGTGATGCAGTGGATCATTTGGTGAACAATGCGGCGATTACCCATTTGGTGATGTTTGAAGACATTGCCGATATTGCTGCGTTCAGACAAATAATGGTAAGAACAAAGCTTGAATACCTAGATATTTTTAAACATCGACGTCTAATAGAAGAGATTTCTATACCCTTGTTGTGTAATGCTTCATTCCCCTACACCTCTATTAGTGAGAGGTTCGTTCTCTTACGCTTCTACTAGGGAGAGGTTTCTACAAGACGGGTACAAGATCTCGTAATCCACCACTTTGGAGGCTTATTGTCCTTTCTGACGCACCATTCCGTGACCGGCTTTGATACCATTTATAACGGCCCAAACCCATGTCTCAGATGCCATTTGTCTTGGATTTTTTCTTCGGGGCTTCCCCTTAAATTTTTTAAAACGAGCATGTCACGTCTGAATTTCTACTCCAATTGACGTGGGATCTCAGAATCCACTTGTTTGAGCATCATACCCCTTCCCCAATCAATGTGCTATTTTGGATTTTTTTGGGTTTTGAACATAAATTTGGGTAAATTGCAGGATATAAACTACTGGGGAGCAGTGTATCTAACCCATATGGCAGTTCCTTATCTGAGATACAGCAGAGGGAAGATCGTGGCGCTGTCAGCTCCTCCGGCGTGGATGCCGGCGCCGAGAATGAGCATTTACAATGTATGTTTTTAAAAACATGGGTTTTGGTGAAATACTATTGAGAGCATAGCTTATAAACGTGTTTGAAAATTGAATGGCAGTCGAGCAAGGCGGCGATTAAGACCATGTTCGAGACACTGAGAGTGGAGCTGGCACCGGAGATCGGTGTCACGATCGTTACTCCTGGCTTCGTTGAATCTGAGTTAACCAAAGGCAAAGCATTGAACGCTCAAGGCAAAATGGAGCTTCGTCAGGACATGAGGGATGTACGCAATTTCAGTCCTTTTCTTTTTCAAATCCTGTAATTATTTAAACCAATTTGAAAGATGTTTAACAAAATGGTGGTGGATGTCAGGCGTTGATAGGGTTGGTACCAGTGGAGACGGCGGAGGCATGCGCGAAGGCGGTGGTGAAGGGGATATGCAGGGGACAGAGGTACGTGACAGAGCCGTCGTGGTACGATGTCCTGTATTACTGGAAGGCGTTTTGCCCGGAGATAGTGGAGTGGTGCTACCGGATATTGGCTATGCCGGCGGCGGGAGCTTCGGAATCGGACGCCCTGACCAAGCAGGCGCTTGATTATACGGGTGCTAAGTATGTGATGTATCCGTCTTCCATTCGCTCCGCCGGCGGTGGCAAGGCCGATTAATCCATGTACAATTTATGAGCTTTTTTTTTTGTATGTTTTTTTTGTTGGCGTTGGAATAATATTGTATTAATTCTCTTCCCTGTTCTTCGCTTATGATTTTTGTACTTTCGTCTGTGTGCTTGCTTGTTTGTAAAATCAAATCTCTCAGCTTAGTTCTTCTATCCTCTTCTACGTTTGGTATTTTATTCCTCCAAGTGTTCGACTAGTCGATTTTTTCAAGGTTAGGATTAAAACCAAAGAGAAGGATCCGACCGTGTATGAATCACGGGATCCCCACCCTCATAGTATTAGACTATATATATTAGTTCTACATATTGTGATATCCTACATCGGTCGGAGGAGAGAACGAAACATTCTTTATAAGAACATGTAAACGAAACATTCCTCATAAGGGCGTGATTATCATCTCTATATAAAGGGCATGTTGTACAAAGGACATGTTCTAAAATCTTGAGTGGAAGTTTGGAAGGGGAAATTCAAACCGTATAATATCTGTTAGTAGTAACTTTGAGAGCGGTGGGTTATTACAATAGTAGTAACTTTGAGAGCTATTAGTAGTAACTTTGAGAGCGGTGGGTTATTACAAATGGTATCAGAGCTAGACACAAGGTTGTGAGCTATTACAAATGGTATCAAATGGGCTGTGAACTATTACAAATGGTATCAGAGCACAACCCTTCCAAATAGGCAGTGAGCAGAGTTCGGCTGTGAGCTATTACAAATGATACTCTGTGAGCTATGACAAATGGTATCAGAGCCAGACATCGAGTGGTGGATTATTACAAATGGTTTCAAAGTCTGGCTTGTGTGGTGGATTATTACAAATGGTTTCAAAGTCAGGGCGGTGAGCACAAATGGTATCACACCCCTTACAAATGGTATTAGAACCAGGTTGTGAGTTATTATAAATGGTATCAGAGCCAAACATCGAGTGATGGATTATTACAAATAGTATCAGAGTCAGACACCAGGCAATAAGCTATTTCAAATGTGCTAGTGAAGACTCCCCCATAAGCTTCTAGACATTAAGTGGTGGGCTATTACAAATAGTATCACTAAACAGTGTGCCAGCGCCCCCCATGAGTTTCTAGACGTCGAGTGGTGTCGAATTACGAACAAGCCACTTCAAATCTGTCCATTTTTAATGTTTTGAGTTAGTTGACTAAGCAGAAGGTGATATGGCAAGTGGCATTTTAAAATGACGTGGAAATTTCTTTTTATCGATCTTAAATAAAATATATAATAATTAGCAACGTGGACTACAGTTATTTGACCTAATTTATGAGTACTCTTACGTGTCGCTTGTTTGGCATGCAATGCTGCCACGTAACATCATCCCTTCTCCTTGGTTTAAACCTCAACTTTTGTTCTCTTCCAAAATAATCCCATGGATTTCATCAACAGTATTTTGAAGGTCATAATCGACCGCATATCTCAAACAGCCTTCCCCTTCATCTACCCAATTTACTTCTTCTTCAAGTTCATTTCCTTCATAATAAGGTCCATTTCCGCCGAAGATGTCGCCGGAAAAGTCGTTCTCATCACCGGAGCATCCTCCGGCATCGGCGAGGTTCAAACACTAAACATTTCAACAAATTGATTTTAATTTCTGTTTGTTTTTTGTTTTGTGGGTTTTGATTGTATTTTGTGAAGCATCTGGCGTACGAGTACGCTAAAAGAGGAGCTTATTTGGCGCTGGTTGCCAGAAGGGATCATCGGCTCCGGGATGTTGCTGATGTGGCAGAGATTTTGGGGTCGCCCTATGCTCTCGTCATCCATGCCGATGTTTCTAACCTTGATGATTGCCAACGATGTGTCGATACAACTGTCGGTCACTTTGGACAATGTAAGTAATATTTTAATATTTTAAATTATCGTGACAGTAGATATTGTTTTATTTGGGTTTACCTTCTAAGCTTACCCTCAATGTGGAGTCATTTACAGATGGATGGGTCGGTCTCTACTCCAACTGTATCTTGCACTGTTGTAGTGACTGGCTCTTATACCATTTGTAACATCCAAATCCACGGCTAACAATGCCCAAATCCACTACTAACAGATATTGTTTGTTTGTGTGCGATCATACAAGCATTAACCCACTTGATCCTTTATTGTCAGATGGGTCTGTCTCTCCTCCAACTAGTGTATCTGACACTTTTATAGTGATTAACTCTAATACTATTGGTAACAGCTCAAATTCACAGCAAACAATATTGTATTTTTTGTGTGCGATCATACTAGTATTAATCCATCTCATCCCATGAGAGTTTCGCAGTTGAGCGTGCTTAAGCGTGAGTAGTACTAAGTTGGGTTCTCTTTAGGTGCGATCATATCAACAACACTAGATTCCATTAGAACTCCTTGCAGATAAGCGTGCTTGAGCGAAAGTAATACTAGGTTGGGTCCTCTTTGGGTGCCATCATATCAGCCCTAATGCACCGGATTTCATTAGAACTCTCGACAATTAAGCGTGTTTGGGTGAGAGCAGTACTAAGTTGGGTCATCTTTAGGTGCAATCATACCAGCATTAATGCACTAGATCTCATTAAAATTACCCAATTAAGTGGGATTGGGCGAGAGTAGTACTAAGCTGGGTGACCACTTGTGAAGTTCTCGTGTTGCAAGTGTAAAGTGGACAATATTTGCTAGGGGTGGATGTAGGCTATTACAATTATAATCGTGTGGATTTCCGCTGTTATAATTATAATCCTTGTGTTCTTGTCTAAATGTTTGTTTTTGTGTGTCTATAATTGTTGGTTGAAACAGTGGATCACTTGGTTAACAATGCTGGGATTACCAGCATCAACTTGTTTGAAGAATATGTGGATCCCAGAAATATTGCATCAGTAATGGTGATGATCATGAATCTCAACAACCCACTTCACTGATTCAACAAAAATGGTTGCAAAATGATGAAAAATTAATCCTTTTGTTCTTGCACAGGACACAGATTTCTGGGGAACGGTTTATTGCACTTACTACGCAATTCCGTACTTAAAACAAACCCGAGGGAAGATCATCGGAATCGCTTCCTCCGCGGCATGGCTCCCCACGCCCCGCCTGAGCTTTTACAGTGTAAAATCCCGATTTCATATCGAATTTCGTGTTATGTTTCTTATGTTCTGATTTTCTATAAATTACTTTATTTAAATTCAAAGGCAAGTAAAGCAGCTGTGATAAGCTTCTATGAGACATTGAGAGTGGAAATTGGAAGTGAGATTGGAATTACGGTTGTGACGCCGGGGTTGATTGAATCGGAGATGACGAAAGGGAAATTCATGTTCGAAGATGGGAAGATGTACATTGATCAAGAATTGAGAGACGCTGTAATGAGCGTGATGCCGATAGAACCGGTGGGCGGTGCGGTGGAAGCGATGTTAAGGGCGGGTTGTAGAGGAGATGAGTACGTGACGGAACCGCCGTGGTTGAGGATGTCGTTTTACTGGAAGATGTTTTTGCCGGAGGTGGTGGAATGGTTGAATTACTTGTTTATAATGACCGGAAGTTCCCCGACCGACACATTTGGGAAATGGCTGCTTGAGCTCACTGGATTGAAGCCCCTTATTTATCCACCTTCTGTTCGATCGCCGGAACTCGGAATACAAATAGGAGTGTACCCAAATATA

mRNA sequence

CTTGAGAATTCTTAGTCCGGCAGCCATGAATATCCTTCACACCTTTCTGAATTTAGTCGCTCCACCCTTTACTTTCATTTCCCTCTTCCTTTTACTACCGCCTTATCAAGCTTTCAAATCATTTCTCTCTTTGATTGGTTTTCTGTTCACGGAAAATGTCGACGGAAAGGTGGTTCTTATCACCGGCGCTTCCTCCGGCATCGGCGAGCATTTGGCGTATGAGTATGCGAAGAGAGGGGCTTGCTTAGTGCTTGTGGCTAGACGAGAGAATCTACTGGAAGAAGTTGCAGACATAGCTCGGTATTATGGATCACCAGATGTAATCACCATTCGAGCTGATGTTTCGAAGTTTGAAGATTGTCGGCGGGTTATGAACGAGACGATGAATCATTTTGGGAGATTGGATCATTTGGTGAACAATGCGGCGATTACCCATTTGGTGATGTTTGAAGACATTGCCGATATTGCTGCCAGAGGGAAGATCGTGGCGCTGTCAGCTCCTCCGGCGTGGATGCCGGCGCCGAGAATGAGCATTTACAATTCGAGCAAGGCGGCGATTAAGACCATGTTCGAGACACTGAGAGTGGAGCTGGCACCGGAGATCGGTGTCACGATCGTTACTCCTGGCTTCGTTGAATCTGAGTTAACCAAAGGCAAAGCATTGAACGCTCAAGGCAAAATGGAGCTTCGTCAGGACATGAGGGATGCGTTGATAGGGTTGGTACCAGTGGAGACGGCGGAGGCATGCGCGAAGGCGGTGGTGAAGGGGATATGCAGGGGACAGAGGTACGTGACAGAGCCGTCGTGGTACGATGTCCTGTATTACTGGAAGGCGTTTTGCCCGGAGATAGTGGAGTGGTGCTACCGGATATTGGCTATGCCGGCGGCGGGAGCTTCGGAATCGGACGCCCTGACCAAGCAGGCGCTTGATTATACGGGTGCTAATATTTTGAAGGTCATAATCGACCGCATATCTCAAACAGCCTTCCCCTTCATCTACCCAATTTACTTCTTCTTCAAGTTCATTTCCTTCATAATAAGGTCCATTTCCGCCGAAGATGTCGCCGGAAAAGTCGTTCTCATCACCGGAGCATCCTCCGGCATCGGCGAGCATCTGGCGTACGAGTACGCTAAAAGAGGAGCTTATTTGGCGCTGGTTGCCAGAAGGGATCATCGGCTCCGGGATGTTGCTGATGTGGCAGAGATTTTGGGGTCGCCCTATGCTCTCGTCATCCATGCCGATGTTTCTAACCTTGATGATTGCCAACGATTGGATCACTTGGTTAACAATGCTGGGATTACCAGCATCAACTTGTTTGAAGAATATGTGGATCCCAGAAATATTGCATCAGTAATGGACACAGATTTCTGGGGAACGGTTTATTGCACTTACTACGCAATTCCGTACTTAAAACAAACCCGAGGGAAGATCATCGGAATCGCTTCCTCCGCGGCATGGCTCCCCACGCCCCGCCTGAGCTTTTACAGTGCAAGTAAAGCAGCTGTGATAAGCTTCTATGAGACATTGAGAGTGGAAATTGGAAGTGAGATTGGAATTACGGTTGTGACGCCGGGGTTGATTGAATCGGAGATGACGAAAGGGAAATTCATGTTCGAAGATGGGAAGATGTACATTGATCAAGAATTGAGAGACGCTGTAATGAGCGTGATGCCGATAGAACCGGTGGGCGGTGCGGTGGAAGCGATGTTAAGGGCGGGTTGTAGAGGAGATGAGTACGTGACGGAACCGCCGTGGTTGAGGATGTCGTTTTACTGGAAGATGTTTTTGCCGGAGGTGGTGGAATGGTTGAATTACTTGTTTATAATGACCGGAAGTTCCCCGACCGACACATTTGGGAAATGGCTGCTTGAGCTCACTGGATTGAAGCCCCTTATTTATCCACCTTCTGTTCGATCGCCGGAACTCGGAATACAAATAGGAGTGTACCCAAATATA

Coding sequence (CDS)

ATGAATATCCTTCACACCTTTCTGAATTTAGTCGCTCCACCCTTTACTTTCATTTCCCTCTTCCTTTTACTACCGCCTTATCAAGCTTTCAAATCATTTCTCTCTTTGATTGGTTTTCTGTTCACGGAAAATGTCGACGGAAAGGTGGTTCTTATCACCGGCGCTTCCTCCGGCATCGGCGAGCATTTGGCGTATGAGTATGCGAAGAGAGGGGCTTGCTTAGTGCTTGTGGCTAGACGAGAGAATCTACTGGAAGAAGTTGCAGACATAGCTCGGTATTATGGATCACCAGATGTAATCACCATTCGAGCTGATGTTTCGAAGTTTGAAGATTGTCGGCGGGTTATGAACGAGACGATGAATCATTTTGGGAGATTGGATCATTTGGTGAACAATGCGGCGATTACCCATTTGGTGATGTTTGAAGACATTGCCGATATTGCTGCCAGAGGGAAGATCGTGGCGCTGTCAGCTCCTCCGGCGTGGATGCCGGCGCCGAGAATGAGCATTTACAATTCGAGCAAGGCGGCGATTAAGACCATGTTCGAGACACTGAGAGTGGAGCTGGCACCGGAGATCGGTGTCACGATCGTTACTCCTGGCTTCGTTGAATCTGAGTTAACCAAAGGCAAAGCATTGAACGCTCAAGGCAAAATGGAGCTTCGTCAGGACATGAGGGATGCGTTGATAGGGTTGGTACCAGTGGAGACGGCGGAGGCATGCGCGAAGGCGGTGGTGAAGGGGATATGCAGGGGACAGAGGTACGTGACAGAGCCGTCGTGGTACGATGTCCTGTATTACTGGAAGGCGTTTTGCCCGGAGATAGTGGAGTGGTGCTACCGGATATTGGCTATGCCGGCGGCGGGAGCTTCGGAATCGGACGCCCTGACCAAGCAGGCGCTTGATTATACGGGTGCTAATATTTTGAAGGTCATAATCGACCGCATATCTCAAACAGCCTTCCCCTTCATCTACCCAATTTACTTCTTCTTCAAGTTCATTTCCTTCATAATAAGGTCCATTTCCGCCGAAGATGTCGCCGGAAAAGTCGTTCTCATCACCGGAGCATCCTCCGGCATCGGCGAGCATCTGGCGTACGAGTACGCTAAAAGAGGAGCTTATTTGGCGCTGGTTGCCAGAAGGGATCATCGGCTCCGGGATGTTGCTGATGTGGCAGAGATTTTGGGGTCGCCCTATGCTCTCGTCATCCATGCCGATGTTTCTAACCTTGATGATTGCCAACGATTGGATCACTTGGTTAACAATGCTGGGATTACCAGCATCAACTTGTTTGAAGAATATGTGGATCCCAGAAATATTGCATCAGTAATGGACACAGATTTCTGGGGAACGGTTTATTGCACTTACTACGCAATTCCGTACTTAAAACAAACCCGAGGGAAGATCATCGGAATCGCTTCCTCCGCGGCATGGCTCCCCACGCCCCGCCTGAGCTTTTACAGTGCAAGTAAAGCAGCTGTGATAAGCTTCTATGAGACATTGAGAGTGGAAATTGGAAGTGAGATTGGAATTACGGTTGTGACGCCGGGGTTGATTGAATCGGAGATGACGAAAGGGAAATTCATGTTCGAAGATGGGAAGATGTACATTGATCAAGAATTGAGAGACGCTGTAATGAGCGTGATGCCGATAGAACCGGTGGGCGGTGCGGTGGAAGCGATGTTAAGGGCGGGTTGTAGAGGAGATGAGTACGTGACGGAACCGCCGTGGTTGAGGATGTCGTTTTACTGGAAGATGTTTTTGCCGGAGGTGGTGGAATGGTTGAATTACTTGTTTATAATGACCGGAAGTTCCCCGACCGACACATTTGGGAAATGGCTGCTTGAGCTCACTGGATTGAAGCCCCTTATTTATCCACCTTCTGTTCGATCGCCGGAACTCGGAATACAAATAGGAGTGTACCCAAATATA

Protein sequence

MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIGEHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETMNHFGRLDHLVNNAAITHLVMFEDIADIAARGKIVALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALNAQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPEIVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQRLDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI
Homology
BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match: Q93W57 (11-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.8e-102
Identity = 185/335 (55.22%), Postives = 238/335 (71.04%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M+++HTFLNL+APPFTF  L   LPP+Q FK FLS++G LF+E+V GKVV+ITGASSGIG
Sbjct: 1   MDLIHTFLNLIAPPFTFFFLLFFLPPFQIFKFFLSILGTLFSEDVAGKVVVITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E LAYEYAKRGACLVL ARRE  L+EVA+ AR  GSPDV+ +RADVSK EDCR+V+++TM
Sbjct: 61  ESLAYEYAKRGACLVLAARRERSLQEVAERARDLGSPDVVVVRADVSKAEDCRKVVDQTM 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
           N FGRLDHLVNNA I  + M E++ DI                           +RG+IV
Sbjct: 121 NRFGRLDHLVNNAGIMSVSMLEEVEDITGYRETMDINFWGYVYMTRFAAPYLRNSRGRIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
            LS+  +WMP PRMS YN+SKAAI   FETLRVE  P+IG+T+VTPGF+ESELT+GK  N
Sbjct: 181 VLSSSSSWMPTPRMSFYNASKAAISQFFETLRVEFGPDIGITLVTPGFIESELTQGKFYN 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
           A G+  + QDMRD  +   P+   E+ A+++V+   RG+RYVTEP+W+ V Y+WK FCPE
Sbjct: 241 A-GERVIDQDMRDVQVSTTPILRVESAARSIVRSAIRGERYVTEPAWFRVTYWWKLFCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANIL 310
           ++EW +R++ + + G  E +   K+ LDYTG   L
Sbjct: 301 VMEWVFRLMYLASPGEPEKETFGKKVLDYTGVKSL 334

BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match: A7LB60 (11-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.9e-97
Identity = 181/335 (54.03%), Postives = 240/335 (71.64%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M+++HTFLNLVAPPFTF  L L LPPY   K  +S++ +L +ENV GKVV ITGASSGIG
Sbjct: 1   MDLIHTFLNLVAPPFTFFFLCLFLPPYWGLKFMVSILSWLLSENVAGKVVHITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E+LAYEYAKRGACL L ARRE  L +VAD AR+ GSPDVI +RADVSK EDC R++++T+
Sbjct: 61  EYLAYEYAKRGACLALSARRETALHQVADTARHLGSPDVIVMRADVSKPEDCMRLIDQTV 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
           NHFGRLDHLVNNAAI+   +FE+  DI+                          +RGKIV
Sbjct: 121 NHFGRLDHLVNNAAISIATLFEETPDISNLRPIMETNFWGSVYTTRYALQHLRKSRGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
            +S+  +W+PAPR  IY++SKAA+ +++ETLRVE+  EIG+TIVTPG++ESE+TKGK L+
Sbjct: 181 VMSSVDSWLPAPRRHIYSASKAALVSLYETLRVEVGSEIGITIVTPGYIESEITKGKFLS 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
           AQG++++ QD+RD  +  VPV +   CA++++K   RG R +T PSW+ + Y  K  CPE
Sbjct: 241 AQGEVDVDQDLRDVEVSAVPVGSVSGCAESIIKSTLRGDRCLTVPSWFRMTYLIKLLCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANIL 310
           ++EW +R+L + A G   SDAL+K+ LD TGA  L
Sbjct: 301 LLEWTFRLLYLTAPGTPTSDALSKKILDATGAKNL 335

BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match: P0DKC5 (11-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 9.5e-97
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M +++ FLNL AP FTF  L   LPP+  FK   S+   +F+EN+ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E LAYEYA RGACL L ARR+N LEEVA+IAR  GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
            HFGRLDHLVNNA +T + MFE+I DI                           + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           A+S+  AW+ APRMS YN+SKAA+ + FET+R+EL  ++ +TIVTPG++ESELT+GK  +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
            +G++ + QDMRD  +G  PV +A  CAK++V G+CR QRYVTEPSW+ V Y WK  CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
           ++EW  R+L M   G SE  AL K+ +D  G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331

BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match: P0DKC6 (11-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 9.5e-97
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M +++ FLNL AP FTF  L   LPP+  FK   S+   +F+EN+ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E LAYEYA RGACL L ARR+N LEEVA+IAR  GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
            HFGRLDHLVNNA +T + MFE+I DI                           + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           A+S+  AW+ APRMS YN+SKAA+ + FET+R+EL  ++ +TIVTPG++ESELT+GK  +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
            +G++ + QDMRD  +G  PV +A  CAK++V G+CR QRYVTEPSW+ V Y WK  CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
           ++EW  R+L M   G SE  AL K+ +D  G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331

BLAST of Cp4.1LG20g02990 vs. ExPASy Swiss-Prot
Match: Q9LUE4 (11-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=HSD6 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.3e-77
Identity = 149/299 (49.83%), Postives = 200/299 (66.89%), Query Frame = 0

Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
           I+  +   ++  A    YP Y   K    I R++ +E+VAGKVV+ITGA+SGIGE LAYE
Sbjct: 7   IINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIGEALAYE 66

Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
           Y KRGAYLALV  R   L  VA +AE+ GSP  L + ADVS L DC+           RL
Sbjct: 67  YGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATVLHFGRL 126

Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
           DHLV NAG+  +  F +  D    +  MD +FWG+VYCT++A PYLK+ RG+I+ IAS  
Sbjct: 127 DHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIVVIASGC 186

Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
            ++ +PRLSFY ASKAAVI+FYETLR E GS+IG+T+V PG+++SEM++GKFM +DGK+ 
Sbjct: 187 GYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMTKDGKLV 246

Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 596
           +D+ELRD  MSV+P+E      +A++R+ CRGD Y+ EP W+      K+F  E  EW+
Sbjct: 247 VDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSEATEWV 305

BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match: KAG7019754.1 (11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1167 bits (3020), Expect = 0.0
Identity = 601/670 (89.70%), Postives = 605/670 (90.30%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG
Sbjct: 47  MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 106

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM
Sbjct: 107 EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 166

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIAA--------------------------RGKIV 180
           NHFGRLDHLVNNAAITHLVMFEDIADIAA                          RGKIV
Sbjct: 167 NHFGRLDHLVNNAAITHLVMFEDIADIAAFRQIMDINYWGAVYLTHMAIPYLRYSRGKIV 226

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN
Sbjct: 227 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 286

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
           AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE
Sbjct: 287 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 346

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFI 360
           IVEWCYRILAMPAAGASESDALTKQALDYTGA    V IDRIS+T FPFIYPIY  FKFI
Sbjct: 347 IVEWCYRILAMPAAGASESDALTKQALDYTGAKY--VTIDRISETVFPFIYPIYLLFKFI 406

Query: 361 SFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEI 420
           SFIIRS SAEDVAGKVVLITGASSGIGEHLAYEYAK GAYLALVARRDHRLR VADVAEI
Sbjct: 407 SFIIRSTSAEDVAGKVVLITGASSGIGEHLAYEYAKTGAYLALVARRDHRLRHVADVAEI 466

Query: 421 LGSPYALVIHADVSNLDDCQRLDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYC 480
           LGSPYALVIHADVSNL+DC+R                   VD        DTDFWGTVYC
Sbjct: 467 LGSPYALVIHADVSNLEDCKRC------------------VDTT------DTDFWGTVYC 526

Query: 481 TYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVV 540
           TYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVV
Sbjct: 527 TYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVV 586

Query: 541 TPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTE 600
           TPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTE
Sbjct: 587 TPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTE 646

Query: 601 PPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPEL 644
           PPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPEL
Sbjct: 647 PPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPEL 690

BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match: KAE8652630.1 (hypothetical protein Csa_013319 [Cucumis sativus])

HSP 1 Score: 724 bits (1870), Expect = 5.42e-254
Identity = 375/678 (55.31%), Postives = 485/678 (71.53%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M++++ FLNL+    +  +   + P Y  FK  L  I   F E+V GKVVLITGASSGIG
Sbjct: 1   MDLINNFLNLIIDRISQTAFSFVFPLYFFFKLLLFPIRSSFAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           EH+AYEYAKRGA L LVARRE+ L EVA++A   GSP  + I ADVS  +DC+R +  T+
Sbjct: 61  EHVAYEYAKRGAYLALVARREHRLREVAEVAEILGSPFALLIHADVSNVDDCKRCVQTTL 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIAD---IAA-----------------------RGKIV 180
            HFGRLDHLVNNA IT + +FE+  D   +A+                       RGKI+
Sbjct: 121 THFGRLDHLVNNAGITSINLFEEYDDPRNVASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
            +++  AW+P PR+S Y++SKAA+ + +ETLRVE+  +IG+TIVTPG  ESE+T+GK + 
Sbjct: 181 GIASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGRDIGITIVTPGLTESEMTQGKFMF 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
             GKM L Q++RDA++ ++P+E   + AKA+V G CRG  YVTEP W  + +YWK F PE
Sbjct: 241 EDGKMYLDQELRDAVMSVMPIEAVGSAAKAIVNGGCRGDEYVTEPPWIRMTFYWKMFLPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYP--IYFFFK 360
           +VEW   +  M   G+S +++  K+ L++TG   LK           PFIYP  +     
Sbjct: 301 MVEWLNYLFIM--TGSSPTESFGKRVLEFTG---LK-----------PFIYPPSLRDGGD 360

Query: 361 FISFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVA 420
            +  I +S+ +EDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARR++RLR+VA VA
Sbjct: 361 IVGSIRKSVFSEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRENRLREVAAVA 420

Query: 421 EILGSPYALVIHADVSNLDDCQR-----------LDHLVNNAGITSINLFEEYVDPRNIA 480
           + LGSPYALVI AD+S ++DC+R           LDHLVNNAG++S+NLFEEY + +N  
Sbjct: 421 QYLGSPYALVIPADISKIEDCKRCIQTTVTHFRRLDHLVNNAGVSSVNLFEEYDNLQNAV 480

Query: 481 SVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETL 540
            VMD +FWG VYC+YY IP+LKQ+RGKIIGIASSAAWLP PRLSFY++SKAAVISFYETL
Sbjct: 481 PVMDVNFWGMVYCSYYGIPHLKQSRGKIIGIASSAAWLPAPRLSFYTSSKAAVISFYETL 540

Query: 541 RVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAM 600
           RVE+G +IGIT+VTPGL+ESEMT+GKFM +DG +Y+DQ+LRDA +S MPI P+  AV+ +
Sbjct: 541 RVEVGRDIGITIVTPGLVESEMTQGKFMSKDGHLYLDQQLRDATVSAMPIMPIDDAVKGI 600

Query: 601 LRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWLNYLFIMTGSS--PTDTFGKWLLELTG 637
           LR+ C+GD Y TEP W++M+FY+K   PE+VEW NYL  M GSS  PTDTFGK L++L+G
Sbjct: 601 LRSVCKGDRYATEPRWMKMAFYYKTLWPELVEWFNYLINMRGSSNSPTDTFGKRLVQLSG 660

BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match: KAG7012327.1 (11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 701 bits (1810), Expect = 1.52e-246
Identity = 367/645 (56.90%), Postives = 448/645 (69.46%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M ++ +FLNLVAPPFT +SL LLLP Y  FK F SL+  LF+E+V GKVVLITGASSGIG
Sbjct: 1   MELIQSFLNLVAPPFTVVSLGLLLPHYVVFKYFFSLVRGLFSEDVTGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E+LAYEYAKRGA L LVARRE+ LEE A+ ARYYGSP+VITI  DVS+  DCRR++++TM
Sbjct: 61  ENLAYEYAKRGAQLALVARRESSLEEAAEAARYYGSPEVITIAGDVSELSDCRRIIDDTM 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIAARGKIVAL----SAPPAWMPAPRMS-----IY 180
           NHFGRLDHLVNNA I ++ +FE+I DI +  KI+      S    W+  P +      I 
Sbjct: 121 NHFGRLDHLVNNAGIANMTLFEEIIDITSFKKIMETNYWGSVYMTWLAIPYLKNRGGKII 180

Query: 181 NSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALNAQGKMELRQDMRDALIG 240
            +SKAA+++ FETLRVE++PEIG+T+VTPGFVESE+TKGKAL + G+ME+ QD+RDAL+G
Sbjct: 181 VASKAALRSFFETLRVEMSPEIGITVVTPGFVESEITKGKALYSHGRMEVHQDVRDALVG 240

Query: 241 LVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPEIVEWCYRILAMPAAGAS 300
            +PVETA ACAKA+V+  C+G RY+TEPSWY+  YY KAFCPE+VEWCYRIL     G S
Sbjct: 241 AIPVETAAACAKAIVRSACQGDRYLTEPSWYNAAYYLKAFCPEVVEWCYRILVYTTPGTS 300

Query: 301 ESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVV 360
            ++AL KQ LD TGA           +TA   +YP                         
Sbjct: 301 AAEALNKQILDLTGA-----------KTA---MYP------------------------- 360

Query: 361 LITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLD 420
                   I  H+AYEYAKRGAYLALVARR++RLR+VA VA   GSP+AL          
Sbjct: 361 ------PSIHSHMAYEYAKRGAYLALVARRENRLREVAGVARFFGSPFAL---------- 420

Query: 421 DCQRLDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIG 480
                                            D +FWG VYCTY+AIP+LKQ+RGKIIG
Sbjct: 421 ---------------------------------DVNFWGMVYCTYFAIPHLKQSRGKIIG 480

Query: 481 IASSAAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFE 540
           I+SSAAWLP PRLSFYSASKAAVISFYETLRVE+G +IGIT+VTPGL+ESEMT+GKFM +
Sbjct: 481 ISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFMSK 540

Query: 541 DGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEV 600
           DG +Y+DQELRDA++S MPI  +  AV  ++R+ CRGD Y TEP W+RM+FY+K   PE+
Sbjct: 541 DGGLYLDQELRDAMVSAMPIMAIDSAVNGIVRSACRGDRYATEPRWMRMAFYYKTLWPEL 557

Query: 601 VEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 636
           VEW+NYL  MT SSPTD+FGK LLELTGLK   YP SVRS +L +
Sbjct: 601 VEWVNYLIGMTSSSPTDSFGKRLLELTGLKTWFYPESVRSQKLWL 557

BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match: XP_023519994.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675 bits (1741), Expect = 2.60e-239
Identity = 337/349 (96.56%), Postives = 338/349 (96.85%), Query Frame = 0

Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
           +ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6   SILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 65

Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
           EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR           
Sbjct: 66  EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQRCVDTTVGHFGQ 125

Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
           LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 185

Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
           AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245

Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
           YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL
Sbjct: 246 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 305

Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 644
           NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 354

BLAST of Cp4.1LG20g02990 vs. NCBI nr
Match: XP_022923773.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata])

HSP 1 Score: 656 bits (1693), Expect = 7.41e-232
Identity = 326/349 (93.41%), Postives = 331/349 (94.84%), Query Frame = 0

Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
           +ILKV IDRISQT FPFIYPIY  FKFISFIIRS SAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6   SILKVTIDRISQTVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIGEHLAY 65

Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
           EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNL+DC+R           
Sbjct: 66  EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLEDCKRCVDTTVSHFGQ 125

Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
           LDHLVNNAGITSINLFEEY DPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 185

Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
           AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245

Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
           YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL
Sbjct: 246 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 305

Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 644
           NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQ+G YPN+
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQVGAYPNM 354

BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match: A0A6J1EAJ7 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111431382 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 3.59e-232
Identity = 326/349 (93.41%), Postives = 331/349 (94.84%), Query Frame = 0

Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
           +ILKV IDRISQT FPFIYPIY  FKFISFIIRS SAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6   SILKVTIDRISQTVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIGEHLAY 65

Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
           EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNL+DC+R           
Sbjct: 66  EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLEDCKRCVDTTVSHFGQ 125

Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
           LDHLVNNAGITSINLFEEY DPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 185

Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
           AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245

Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
           YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL
Sbjct: 246 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 305

Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQIGVYPNI 644
           NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQ+G YPN+
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQVGAYPNM 354

BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match: A0A6J1KHB1 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111495256 PE=3 SV=1)

HSP 1 Score: 643 bits (1659), Expect = 2.89e-227
Identity = 319/343 (93.00%), Postives = 327/343 (95.34%), Query Frame = 0

Query: 307 NILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAY 366
           N+LK+IIDRISQTAFPFIYPIY  FKFI FIIRS SAEDVAGKVVLITGASSGIGEHLAY
Sbjct: 6   NLLKIIIDRISQTAFPFIYPIYLLFKFIFFIIRSTSAEDVAGKVVLITGASSGIGEHLAY 65

Query: 367 EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQR----------- 426
           EYAKRGAYLALVARRDHRLRDVADVAEILGSPYAL+IHADVSNL+DC+R           
Sbjct: 66  EYAKRGAYLALVARRDHRLRDVADVAEILGSPYALLIHADVSNLEDCKRCVDTTVSHFGR 125

Query: 427 LDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASS 486
           LDHLVNNAGITSINLFEEY DPRNIASVMDTDFWGTVYCTY+AIPYLKQTRGKIIGIASS
Sbjct: 126 LDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYFAIPYLKQTRGKIIGIASS 185

Query: 487 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 546
           AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM
Sbjct: 186 AAWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKM 245

Query: 547 YIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 606
           YIDQELRDAV+SVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEW+
Sbjct: 246 YIDQELRDAVISVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWM 305

Query: 607 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQI 638
           NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQI
Sbjct: 306 NYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGIQI 348

BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match: A0A2U1MAX7 (Hydroxysteroid dehydrogenase 6 OS=Artemisia annua OX=35608 GN=CTI12_AA403160 PE=4 SV=1)

HSP 1 Score: 620 bits (1598), Expect = 1.57e-211
Identity = 339/760 (44.61%), Postives = 458/760 (60.26%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M+ + + LNLVAP  T  +LF   P Y  FK    +   +F E+V GKVVLI GASSGIG
Sbjct: 1   MDFVDSVLNLVAPIATICALFCFTPLYIGFKLLWFIFRSIFKEDVAGKVVLIVGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           EH+AYEY+KRGACLVL ARR++ L+EVAD A   G+PD I IRADVS  EDC+R++N+T+
Sbjct: 61  EHVAYEYSKRGACLVLAARRKHSLQEVADTAVSLGAPDAIAIRADVSNIEDCKRLINKTI 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
           +HFG+LDHLVN A IT + M EDI DI                           ++GKIV
Sbjct: 121 SHFGKLDHLVNCAGITSMSMLEDITDITRFTQVLDTNFWGYVYMTHLSIPHLRRSKGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           A+++   WMP PRM+IYN+SKAA+ + +E+LR+EL  E+G+TIVTPG  ESE+++GK ++
Sbjct: 181 AIASSATWMPVPRMNIYNASKAAVVSFYESLRMELGSEVGITIVTPGLTESEMSQGKIMD 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
             G+M + QDMRDA + +VP+E A   A A+V G  RG   +  P W     YW AF PE
Sbjct: 241 KDGEMVIDQDMRDAEMSIVPIELAAKSAVAIVDGASRGDLSLAVPRWVQTTIYWVAFFPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGAN-----------ILK------------- 360
           +++W      +   GAS  DA +K  LD  G             ++K             
Sbjct: 301 MIDWVNHWFLLAEPGASPLDAPSKMLLDVPGLRHIVQPDSVISPVIKEYDYKLVVHTPHK 360

Query: 361 --------------------------------------------VIIDRISQTAFPFI-- 420
                                                       +II  ++Q    F+  
Sbjct: 361 KRSDVVAVLIIPLEMLSRSCVIPSCVPFFVDIALLTDKPDSRHLIIIINLNQPDMDFVDS 420

Query: 421 -----------------YPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 480
                             P+Y  FK + +I RSIS EDVAGKVVLI GASSGIGEH+AYE
Sbjct: 421 VLNLVAPIATICALFCFTPLYIGFKVLWYIFRSISKEDVAGKVVLIVGASSGIGEHVAYE 480

Query: 481 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQRL----------- 540
           YAKRGA L L ARR H L++VAD A  LG+P A+ I ADVSN++DC+RL           
Sbjct: 481 YAKRGACLVLAARRKHSLQEVADTAVSLGAPDAIAIRADVSNIEDCKRLINKTISHFGKL 540

Query: 541 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 600
           DHLVN AGITS+++ E+  D      VMDT+FWG VY TY +IP+L++++GKI+ IASSA
Sbjct: 541 DHLVNCAGITSMSMLEDITDITRFTQVMDTNFWGYVYMTYLSIPHLRRSKGKIVVIASSA 600

Query: 601 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 634
            W+P PR++ Y+ASKAAV+SFYE+LR+E+GSE+GIT+VTPGL ESEM++GK M +DG+M 
Sbjct: 601 TWMPVPRMNIYNASKAAVVSFYESLRMELGSEVGITIVTPGLTESEMSQGKIMDKDGEMV 660

BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match: A0A4Y1RH78 (Hydroxysteroid dehydrogenase 1 OS=Prunus dulcis OX=3755 GN=Prudu_014581 PE=4 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 1.52e-210
Identity = 328/651 (50.38%), Postives = 431/651 (66.21%), Query Frame = 0

Query: 2   NILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIGE 61
           N++H FLNLVAPPFTF SL L LPP+  FKSFLS++  +F+ENV+GKVVLITGASSGIGE
Sbjct: 5   NLIHKFLNLVAPPFTFFSLCLFLPPFYLFKSFLSVLSSIFSENVNGKVVLITGASSGIGE 64

Query: 62  HLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETMN 121
           HLAYEYAKRGA LVLV RRE+ L EV D A   GSPDV+ +RADVSK EDC+R+++ET+N
Sbjct: 65  HLAYEYAKRGARLVLVGRREDSLREVVDRASDRGSPDVLMVRADVSKVEDCKRIVDETIN 124

Query: 122 HFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIVA 181
           HFGRLDHLVNNA IT L   E+  DI                           ++GKIV 
Sbjct: 125 HFGRLDHLVNNAGITSLGRLEEATDITNFRAIMDTNFWGSVYTTRFAIPHLRCSKGKIVV 184

Query: 182 LSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALNA 241
           LS+  +W+P PR SIYN+SKAA+ +M+ETLR+E  P I +TIVTPG++ESELT+GK L +
Sbjct: 185 LSSAASWLPIPRQSIYNASKAALLSMYETLRIEFGPNIQITIVTPGYIESELTQGKFLMS 244

Query: 242 Q-GKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 301
             G+M + QD+RDA +   PV + E CAKA+V  +CRG RY+TEP W+ V Y WK FCPE
Sbjct: 245 DDGEMIVDQDLRDAQVSATPVGSVEGCAKAIVNSVCRGDRYLTEPEWFGVTYIWKVFCPE 304

Query: 302 IVEWCYRILAMPAAGASESDALTKQALDYTGANILKVIIDRISQTAFPFIYPIYFFFKFI 361
           ++EW YR++ +   G+S  +A +K+ LDYTGA    V+      +            +FI
Sbjct: 305 LLEWGYRLMYVTRPGSSAKEAPSKKILDYTGAE--NVLYPETLHSP-----------EFI 364

Query: 362 SFIIRSISAEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRDHRLRDVADVAEI 421
           SF+ RS S E++ GKVVLITGASSGIGEHLAYEYAKRGA LAL ARR+ RLR VAD A  
Sbjct: 365 SFMRRSKSIENMGGKVVLITGASSGIGEHLAYEYAKRGARLALAARREDRLRAVADKARG 424

Query: 422 LGSPYALVIHADVSNLDDCQRL-----------DHLVNNAGITSINLFEEYVDPR--NIA 481
           LGSP A+V+ ADVS L+ C RL           DHLVNNAG+     FE++   +   + 
Sbjct: 425 LGSPDAIVLPADVSILEHCNRLVNETVNHFGQLDHLVNNAGVLQAGSFEDWTQGQFSKLV 484

Query: 482 SVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSAAWLPTPRLSFYSASKAAVISFYETL 541
           S+MD +FWG+VYCT++++PYL++++GKI+ I+S A+W   P+          +  F+ETL
Sbjct: 485 SIMDINFWGSVYCTHFSLPYLRKSKGKIVVISSFASWFSAPK---------QLTCFFETL 544

Query: 542 RVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMYIDQELRDAVMSVMPIEPVGGAVEAM 601
           R E+GS+IGIT+V+PG+IESEMT              Q L     ++MP +   G  +A+
Sbjct: 545 RAELGSDIGITIVSPGVIESEMTGSS-----------QFLSQVRSNLMPSKSTEGCTKAI 604

Query: 602 LRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWLNYLFIMTGSSPTDTFGK 612
           +    RGD Y+TEP W++  F+ ++F PE++E L  L + T  +P  T  K
Sbjct: 605 VDRAWRGDMYLTEPAWVKFGFWLRVFCPELLEKLFQL-MATKKNPAGTSIK 621

BLAST of Cp4.1LG20g02990 vs. ExPASy TrEMBL
Match: A0A6J1EAX4 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111431503 PE=3 SV=1)

HSP 1 Score: 589 bits (1519), Expect = 5.56e-206
Identity = 305/332 (91.87%), Postives = 305/332 (91.87%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG
Sbjct: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM
Sbjct: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIAA--------------------------RGKIV 180
           N FGRLDHLVNNAAITHLVMFEDIADIAA                          RGKIV
Sbjct: 121 NRFGRLDHLVNNAAITHLVMFEDIADIAAFRQIMDINYWGAVYLTHMAIPYLRYSRGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN
Sbjct: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
           AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE
Sbjct: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGA 306
           IVEWCYRILAMPAAGASESDALTKQALDYTGA
Sbjct: 301 IVEWCYRILAMPAAGASESDALTKQALDYTGA 332

BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match: AT5G50600.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 355.9 bits (912), Expect = 6.8e-98
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M +++ FLNL AP FTF  L   LPP+  FK   S+   +F+EN+ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E LAYEYA RGACL L ARR+N LEEVA+IAR  GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
            HFGRLDHLVNNA +T + MFE+I DI                           + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           A+S+  AW+ APRMS YN+SKAA+ + FET+R+EL  ++ +TIVTPG++ESELT+GK  +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
            +G++ + QDMRD  +G  PV +A  CAK++V G+CR QRYVTEPSW+ V Y WK  CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
           ++EW  R+L M   G SE  AL K+ +D  G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331

BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match: AT5G50700.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 355.9 bits (912), Expect = 6.8e-98
Identity = 177/331 (53.47%), Postives = 230/331 (69.49%), Query Frame = 0

Query: 1   MNILHTFLNLVAPPFTFISLFLLLPPYQAFKSFLSLIGFLFTENVDGKVVLITGASSGIG 60
           M +++ FLNL AP FTF  L   LPP+  FK   S+   +F+EN+ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGACLVLVARRENLLEEVADIARYYGSPDVITIRADVSKFEDCRRVMNETM 120
           E LAYEYA RGACL L ARR+N LEEVA+IAR  GSP+V+T+ ADVSK +DCRR++++T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 NHFGRLDHLVNNAAITHLVMFEDIADIA--------------------------ARGKIV 180
            HFGRLDHLVNNA +T + MFE+I DI                           + GKIV
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ALSAPPAWMPAPRMSIYNSSKAAIKTMFETLRVELAPEIGVTIVTPGFVESELTKGKALN 240
           A+S+  AW+ APRMS YN+SKAA+ + FET+R+EL  ++ +TIVTPG++ESELT+GK  +
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 AQGKMELRQDMRDALIGLVPVETAEACAKAVVKGICRGQRYVTEPSWYDVLYYWKAFCPE 300
            +G++ + QDMRD  +G  PV +A  CAK++V G+CR QRYVTEPSW+ V Y WK  CPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 IVEWCYRILAMPAAGASESDALTKQALDYTG 306
           ++EW  R+L M   G SE  AL K+ +D  G
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPG 331

BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match: AT5G50770.1 (hydroxysteroid dehydrogenase 6 )

HSP 1 Score: 292.4 bits (747), Expect = 9.2e-79
Identity = 149/299 (49.83%), Postives = 200/299 (66.89%), Query Frame = 0

Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
           I+  +   ++  A    YP Y   K    I R++ +E+VAGKVV+ITGA+SGIGE LAYE
Sbjct: 7   IINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIGEALAYE 66

Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
           Y KRGAYLALV  R   L  VA +AE+ GSP  L + ADVS L DC+           RL
Sbjct: 67  YGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATVLHFGRL 126

Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
           DHLV NAG+  +  F +  D    +  MD +FWG+VYCT++A PYLK+ RG+I+ IAS  
Sbjct: 127 DHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIVVIASGC 186

Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
            ++ +PRLSFY ASKAAVI+FYETLR E GS+IG+T+V PG+++SEM++GKFM +DGK+ 
Sbjct: 187 GYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMTKDGKLV 246

Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVVEWL 596
           +D+ELRD  MSV+P+E      +A++R+ CRGD Y+ EP W+      K+F  E  EW+
Sbjct: 247 VDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSEATEWV 305

BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match: AT5G50590.1 (hydroxysteroid dehydrogenase 4 )

HSP 1 Score: 250.8 bits (639), Expect = 3.1e-66
Identity = 132/296 (44.59%), Postives = 184/296 (62.16%), Query Frame = 0

Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
           I  +++  ++ +      P   FFK + FI     +E V GKVV+ITG+SSGIGEHLAYE
Sbjct: 7   IFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIGEHLAYE 66

Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
           YA+RGAYL LVARR+ RL+ VAD    LGSP   V+  DVS + DC+           RL
Sbjct: 67  YARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETISRFGRL 126

Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
           DHLVNNAGI     FE+Y +  ++  +++T+FWG VY T++AIP+LK+T+GKII +AS A
Sbjct: 127 DHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGKIIAVASPA 186

Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
            W   PR+S Y+ASKAA+I+FYETLR+E+  E+G+T+V PGLIE+  T    + E     
Sbjct: 187 GWSGVPRMSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLAE----- 246

Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVV 593
                +     V+ IE      +A++   CRG  +V EP W+R+ F+     PE++
Sbjct: 247 -----KQDWSQVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPELL 292

BLAST of Cp4.1LG20g02990 vs. TAIR 10
Match: AT5G50690.1 (hydroxysteroid dehydrogenase 7 )

HSP 1 Score: 250.8 bits (639), Expect = 3.1e-66
Identity = 132/296 (44.59%), Postives = 184/296 (62.16%), Query Frame = 0

Query: 308 ILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIGEHLAYE 367
           I  +++  ++ +      P   FFK + FI     +E V GKVV+ITG+SSGIGEHLAYE
Sbjct: 7   IFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIGEHLAYE 66

Query: 368 YAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQ-----------RL 427
           YA+RGAYL LVARR+ RL+ VAD    LGSP   V+  DVS + DC+           RL
Sbjct: 67  YARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETISRFGRL 126

Query: 428 DHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKIIGIASSA 487
           DHLVNNAGI     FE+Y +  ++  +++T+FWG VY T++AIP+LK+T+GKII +AS A
Sbjct: 127 DHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGKIIAVASPA 186

Query: 488 AWLPTPRLSFYSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFEDGKMY 547
            W   PR+S Y+ASKAA+I+FYETLR+E+  E+G+T+V PGLIE+  T    + E     
Sbjct: 187 GWSGVPRMSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLAE----- 246

Query: 548 IDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEVV 593
                +     V+ IE      +A++   CRG  +V EP W+R+ F+     PE++
Sbjct: 247 -----KQDWSQVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPELL 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93W575.8e-10255.2211-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 S... [more]
A7LB601.9e-9754.0311-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1... [more]
P0DKC59.5e-9753.4711-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 ... [more]
P0DKC69.5e-9753.4711-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 ... [more]
Q9LUE41.3e-7749.8311-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Match NameE-valueIdentityDescription
KAG7019754.10.089.7011-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. ... [more]
KAE8652630.15.42e-25455.31hypothetical protein Csa_013319 [Cucumis sativus][more]
KAG7012327.11.52e-24656.9011-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023519994.12.60e-23996.5611-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo][more]
XP_022923773.17.41e-23293.4111-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1EAJ73.59e-23293.4111-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KHB12.89e-22793.0011-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A2U1MAX71.57e-21144.61Hydroxysteroid dehydrogenase 6 OS=Artemisia annua OX=35608 GN=CTI12_AA403160 PE=... [more]
A0A4Y1RH781.52e-21050.38Hydroxysteroid dehydrogenase 1 OS=Prunus dulcis OX=3755 GN=Prudu_014581 PE=4 SV=... [more]
A0A6J1EAX45.56e-20691.8711-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G50600.16.8e-9853.47hydroxysteroid dehydrogenase 1 [more]
AT5G50700.16.8e-9853.47hydroxysteroid dehydrogenase 1 [more]
AT5G50770.19.2e-7949.83hydroxysteroid dehydrogenase 6 [more]
AT5G50590.13.1e-6644.59hydroxysteroid dehydrogenase 4 [more]
AT5G50690.13.1e-6644.59hydroxysteroid dehydrogenase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 350..367
score: 45.35
coord: 507..524
score: 27.38
coord: 461..477
score: 32.16
coord: 414..425
score: 56.26
coord: 487..506
score: 35.51
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 414..425
score: 55.27
coord: 467..475
score: 44.89
coord: 487..506
score: 39.39
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 48..146
e-value: 3.3E-22
score: 78.9
coord: 349..527
e-value: 6.4E-37
score: 126.9
coord: 149..211
e-value: 5.9E-10
score: 38.9
NoneNo IPR availableGENE3D3.40.50.720coord: 147..285
e-value: 9.1E-18
score: 66.3
coord: 40..146
e-value: 2.7E-30
score: 107.4
coord: 336..602
e-value: 1.3E-54
score: 187.2
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 352..543
e-value: 4.0E-14
score: 50.3
NoneNo IPR availablePANTHERPTHR43391:SF6811-BETA-HYDROXYSTEROID DEHYDROGENASE 1B-LIKE ISOFORM X1coord: 1..148
NoneNo IPR availablePANTHERPTHR43391:SF6811-BETA-HYDROXYSTEROID DEHYDROGENASE 1B-LIKE ISOFORM X1coord: 307..629
NoneNo IPR availablePANTHERPTHR43391RETINOL DEHYDROGENASE-RELATEDcoord: 149..305
NoneNo IPR availablePANTHERPTHR43391:SF6811-BETA-HYDROXYSTEROID DEHYDROGENASE 1B-LIKE ISOFORM X1coord: 149..305
NoneNo IPR availablePANTHERPTHR43391RETINOL DEHYDROGENASE-RELATEDcoord: 307..629
NoneNo IPR availablePANTHERPTHR43391RETINOL DEHYDROGENASE-RELATEDcoord: 1..148
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 474..502
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 158..186
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 345..600
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 43..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g02990.1Cp4.1LG20g02990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity