Cp4.1LG20g02610 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g02610
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Cp4.1LG20: 1440347 .. 1443813 (-)
RNA-Seq Expression	Cp4.1LG20g02610
Synteny	Cp4.1LG20g02610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGAGATTGAAAAGGGAACACCCAAAAGTTGGTTAGGGTAACTATTTTAGGGGAGAGCAGGGAAATGGGATGGTCTTGGGAGTAGGAGATAAAACTTTCAGGGTTTGTTTTTCTTTTATCTTTCATTTCTTGGTGGTATAAATATTAAGGTTTGATGTTTTTATGTGGAAGAGAGAGAGAGAGAGAGCGAGAGGTTGAAGGGTAGGGTGATGAAGGAGACTCTAAGGTATTTGGTTGGAATTGCAGGCCCAAGTGGGTATGGCTCTAAGTCAAGTGCAGAGCAAGTTACTGAACATTTGCAGTGTTCTTCTCCTTTGACTGCCATTATCACTGGTAAGCTTTTATTTTGAACGATAGTAACCTAATTGTGGTCTTTTCTTACTGTTTTGTTCTATATTCACTTTGTTCTGCAAGTTGTATCCTAACTAATATATCGTACTTTAAACGGTCCCCAACTTCAGTGGTTGACTTTTTTTTTTTCTAAAAGAATGAGAAACATAAGTCAATTACGGTTAAGCTATGCTCGAATTATCTTTAAAGTTGTAGTCAGTTAAAAAAAGAATCACAAAAAGGGTTTGAACCAAAAGTTGCAAGAATTTAGGTTTTTCTTTCTATTTTCCTAAATTTTATTAGCAAGAAATTAAATCTATAAAATCTTGTTCGATAATCATATATTTTAGCTTTTTTTTATTTTATTTTATTTTTATTTTTAATTAAGCTTATAAATATTACTGAACCCCTAATTTCTTTGTTTCGTTATATATATATTTTAATGAGCTAGAATTTAATTTATTAAAGAGAGACAAAAATAAAAAAGAAATCGTGAGAAAACAAGCCCAATTTTCAAAAATAAAAATCATTATAAAATGGAGTCTTACCTTTTGATCAATTGAATTATGGTCGGCTTGGTTGTCTTCAGCTCGAGGCTTCATATCTCTAAATTTGAGTCATAAGGATGTTCGAAACTAAATGGATTTAACCAAATCGTTTCTTGTATCCACCTCCAAACCTTAATCCGTACATCCTATACATATATACACATACTTATATGAGAAGCTTAATTCTATAAATATAAGGAAGGAAATAAGGAGAGAAACCACTCAGCATGATCAATTAAACCATATAATGTTACCAAAGAATAATAGATGGGTGTTAAAACAAGACAAAACAATCCTGAAGAATGATGAGAATTTGATCTTATTTGTGGGTGCAAAAAGGAGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTTTTAGCAAAAAGAGGAATGAGAATAGTAATGGCAGCAAGAGACATGAAAAAGGCAGCAAAAGTGAAAGAGAGGATCAAAAGAGAAGGTCCAGAAGCAGAGATTTTAGTGTCTGAGATAGACCTCAGCTCTTTGGCTTCTGTTAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATGTGAGTTTGCCCCCACTTTCCCCAACCAATATCCCATTTTTCCCTCTCAAATTTCGCCCTTTTTGGACACATAAACTGTTTTTGCAGAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGATGACAAGCTTGAATTGACATTTGCTACTAATTATTTGGGTAATAGTTTTTCCTTCTCTCTTTTTTCTTCTCATTCTGAAACTCAAACACAGAACAACAGTTGGCTTGCAGAAGAATAGATGACTCTTAGCTCTACAAACTCCTTAACTTAATTACTTTTTCAGAGTCAGAGAAAGTTGAATCTACTCAATAATGGCTAAAGAAGAATGAAGTTACGTCAAAAAAAGGATACAAAATGAATTTCAAAATTCTTTTTCCTGCAGGACATTACATTATGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAAGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTGAAGAAGGACGATCTCTGCTTCACTCACCTGCTCAACCCAAACAAGTTAGAAAAACTCGAACCCATTACAGATTATTTTCGTTTAGGTTTCAATTTTTCGAGTATGTGTTTATTTGATTCATGAATGGTCGAAACGCAAACTTTCAATTTTGTGTCCAATAAGTCAATAAACTTTCATTTAACAAATCCCTTTTGGGTAACTAAAACTTTTAAGAAATCCCTTTTGAGTAACTAAAACTTGTAATTGAACCCTTTCTTTCTCCCTTTTCTCAAAACACAAAAAGTTACCAGGGTGTTCACTAGCTGGCTACAGGTCAATAGCAATCCATGGTCAGAGAATCAAACTAAACCCTGTTTGGCGTTTTGGATTTGGAATTTCTGCAGGTACAATGGCACGCGTGCTTATGCTCAATCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGTAATCTTGCTTCTTCACCTTCTACGTTTCCTGCAAACAGTTAACAAAACAGTCCATATTCTGAAATTCCAGCAATTTTTACAGGAAAGAGATGCCAATGTGACAATTAATGCAGTGCATCCAGGAATTGTAAAGACTGCAATTCTCAAGGCACATCGAGGATTTATCACAGGCATGAACTGTTGACTCTTTCTAATTCCTGATGTACAGCCATCTTGAGCCAGTTCTACTTCTACCAAAAGGAAAATGTACAAGAGGCTACTGATTTATCATAACTATATTCTAACATCTCTTGCAGATTCTCTCTTTTTCATGGCATCAAAGTTACTAAAATCCATTCCTCAGGTATTTTCTTATGTAAAATCGATTCACAGCGATTGGATTTGCCTAGTGTTACTCATACAAAACAAATAGTAACAATTATTGTGCCTGCAGGGTGCTGCTACGACATGCTATGTGGCTCTAAGCCCACAAACACGTAGAAAAACAGGAAGATATTTTGCAGATTGTAATGAGACTAAGTGTTCATCTCTAGCCAACGATGAGTTAGCAGCACGAAAGCTATGGATACAAACTCGTGCCCTAATTCTCAGAGAACTTGATGAACCAGTTTGAGCTACATCATCAATAAATTTTCAACCAAGAGAGAGAGAGAACAGTATATATAAATCTATGCATTTGTAAATGAACAGAGATCCTGCAGAAGTTCATCAAATAAGATCTCGTAATAAAAATACGCCTAAATTTTTGAACAATCACTCGATTTTTTAGAAGTTGAACTTTATATATGAAGGATCATTTCTAAACGATAACGGAGACAATATGAAATTAACACCGTGTTATCAGGATCTCCGATTGATCAGAGAAAAAAAAAAACAAGAATAATCTTTCATGGAACAAATTACAACCAGTTAGTTAATTTCAAATTCGAACAGGTTACAGTACAGTAAGAAAGAATTGTCCCGGCGTAGACATTGTCGCGCCAGCCAGCTTCGCCGCTGAAGTGAAGTCTCTCCCTCCACCGCCATTTACGATCTCCTCGCATTTTAAGCCCA

mRNA sequence

TTGAGATTGAAAAGGGAACACCCAAAAGTTGGTTAGGGTAACTATTTTAGGGGAGAGCAGGGAAATGGGATGGTCTTGGGAGTAGGAGATAAAACTTTCAGGGTTTGTTTTTCTTTTATCTTTCATTTCTTGGTGGTATAAATATTAAGGTTTGATGTTTTTATGTGGAAGAGAGAGAGAGAGAGAGCGAGAGGTTGAAGGGTAGGGTGATGAAGGAGACTCTAAGGTATTTGGTTGGAATTGCAGGCCCAAGTGGGTATGGCTCTAAGTCAAGTGCAGAGCAAGTTACTGAACATTTGCAGTGTTCTTCTCCTTTGACTGCCATTATCACTGGAGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTTTTAGCAAAAAGAGGAATGAGAATAGTAATGGCAGCAAGAGACATGAAAAAGGCAGCAAAAGTGAAAGAGAGGATCAAAAGAGAAGGTCCAGAAGCAGAGATTTTAGTGTCTGAGATAGACCTCAGCTCTTTGGCTTCTGTTAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGATGACAAGCTTGAATTGACATTTGCTACTAATTATTTGGGACATTACATTATGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAAGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTACAATGGCACGCGTGCTTATGCTCAATCAAAACTGGCTTGCATTTTACATGCCAAGGAGATTCTCTCTTTTTCATGGCATCAAAGTTACTAAAATCCATTCCTCAGGGTGCTGCTACGACATGCTATGTGGCTCTAAGCCCACAAACACGTAGAAAAACAGGAAGATATTTTGCAGATTGTAATGAGACTAAGTGTTCATCTCTAGCCAACGATGAGTTAGCAGCACGAAAGCTATGGATACAAACTCGTGCCCTAATTCTCAGAGAACTTGATGAACCAGTTTGAGCTACATCATCAATAAATTTTCAACCAAGAGAGAGAGAGAACAGTATATATAAATCTATGCATTTGTAAATGAACAGAGATCCTGCAGAAGTTCATCAAATAAGATCTCGTAATAAAAATACGCCTAAATTTTTGAACAATCACTCGATTTTTTAGAAGTTGAACTTTATATATGAAGGATCATTTCTAAACGATAACGGAGACAATATGAAATTAACACCGTGTTATCAGGATCTCCGATTGATCAGAGAAAAAAAAAAACAAGAATAATCTTTCATGGAACAAATTACAACCAGTTAGTTAATTTCAAATTCGAACAGGTTACAGTACAGTAAGAAAGAATTGTCCCGGCGTAGACATTGTCGCGCCAGCCAGCTTCGCCGCTGAAGTGAAGTCTCTCCCTCCACCGCCATTTACGATCTCCTCGCATTTTAAGCCCA

Coding sequence (CDS)

ATGAAGGAGACTCTAAGGTATTTGGTTGGAATTGCAGGCCCAAGTGGGTATGGCTCTAAGTCAAGTGCAGAGCAAGTTACTGAACATTTGCAGTGTTCTTCTCCTTTGACTGCCATTATCACTGGAGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTTTTAGCAAAAAGAGGAATGAGAATAGTAATGGCAGCAAGAGACATGAAAAAGGCAGCAAAAGTGAAAGAGAGGATCAAAAGAGAAGGTCCAGAAGCAGAGATTTTAGTGTCTGAGATAGACCTCAGCTCTTTGGCTTCTGTTAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGATGACAAGCTTGAATTGACATTTGCTACTAATTATTTGGGACATTACATTATGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAAGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTACAATGGCACGCGTGCTTATGCTCAATCAAAACTGGCTTGCATTTTACATGCCAAGGAGATTCTCTCTTTTTCATGGCATCAAAGTTACTAAAATCCATTCCTCAGGGTGCTGCTACGACATGCTATGTGGCTCTAAGCCCACAAACACGTAGAAAAACAGGAAGATATTTTGCAGATTGTAATGAGACTAAGTGTTCATCTCTAGCCAACGATGAGTTAGCAGCACGAAAGCTATGGATACAAACTCGTGCCCTAATTCTCAGAGAACTTGATGAACCAGTTTGA

Protein sequence

MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGMRIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHCWVQWHACLCSIKTGLHFTCQGDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSSLANDELAARKLWIQTRALILRELDEPV

Homology

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.1e-49
Identity = 125/304 (41.12%), Postives = 165/304 (54.28%), Query Frame = 0


Query: 8   LVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGMRIVMAAR 67
           + G AG SGYGS S+AE VT  +  +  LTAIITG TSGIG E ARVL  RG  +++A+R
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSID-AKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASR 66

Query: 68  DMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 127
           + K A   KE I +  P A I   ++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHCWVQWHAC 187
             + S+D +E  FATN++GH+++T LLLDK+  +++ +GI+GRI+N+SS+ H +      
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 LC-------------------------------------------SIKTGL-------HF 247
           +                                            S+  GL       H 
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHS 246

Query: 248 TCQGDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSSLANDELAA 262
                 L  M+  L K+IPQGAATTCYVAL P  +  TG+YFADCN T  S+ A D   A
Sbjct: 247 GLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTLA 306

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.4e-49
Identity = 125/304 (41.12%), Postives = 164/304 (53.95%), Query Frame = 0


Query: 8   LVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGMRIVMAAR 67
           + G AG SGYGS S+AE VT  +  +  LTAIITG TSGIG E ARVL  RG  +++AAR
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSID-AKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAAR 66

Query: 68  DMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 127
           + K A   KE I +  P A I   ++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHCWVQWHAC 187
             + S+D +E  FATN++GH+++T LLLDK+  +++ +GI+GRI+N+SS+ H +      
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 LC-------------------------------------------SIKTGL-------HF 247
           +                                            S+  GL       H 
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHS 246

Query: 248 TCQGDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSSLANDELAA 262
                 L  M+  L K+IPQGAATTCYVAL P  +  TG+YF DCN T  S+ A D   A
Sbjct: 247 GLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTLA 306

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.1e-49
Identity = 121/306 (39.54%), Postives = 173/306 (56.54%), Query Frame = 0


Query: 7   YLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGMRIVMAA 66
           +  G  G SG+ S+S+AE+VT H    + LTAI+TGA+SGIG ETARVL+ RG+ +VMA 
Sbjct: 2   WFFGSKGASGFSSRSTAEEVT-HGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 67  RDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFS 126
           R+    AKVKE I ++ P A++ V E+DLSS+ SVR+F  E+ + GLPLN+LINN G+ +
Sbjct: 62  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 121

Query: 127 PNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHCW----- 186
                S D +EL FATN+LGH+++T+LLLD +  TS+ +  +GRI+N+SS  H +     
Sbjct: 122 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 181

Query: 187 ----------------VQWHACLCSI-----------KTGLHFTCQ-------------- 246
                               + LC++           + G++ T                
Sbjct: 182 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 241

Query: 247 -----GDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSSLANDEL 262
                  ++  +A  +LKS+PQGAATTCYVAL+PQ    +G YF D N  K   L  D  
Sbjct: 242 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 301

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.1e-45
Identity = 115/299 (38.46%), Postives = 165/299 (55.18%), Query Frame = 0


Query: 13  GPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGMRIVMAARDMKKA 72
           G SG+   S+AEQVT  +  +  LTAI+TGA+SGIG ET RVLA RG  ++M  R+M  A
Sbjct: 9   GVSGFSGSSTAEQVTHGIDATG-LTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  AKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYS 132
             VK+ I ++ P A++   E+DLSSL SV++F  EF + G PLNILINN G+ +   + S
Sbjct: 69  KDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLS 128

Query: 133 DDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVH------------- 192
            D +EL FATN++GH+++T LLLD + +T++ +  +GRI+NV+S  H             
Sbjct: 129 KDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKI 188

Query: 193 ----CWVQWHAC----LCSI-----------KTGLHFTCQG-----------------DS 252
                +  W A     L ++           + G++ T                    + 
Sbjct: 189 NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNSAVNG 248

Query: 253 LFFMASKL-LKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSSLANDELAARKLW 262
           L  +  KL LK++ QGAATTCYVAL PQ +  +G YF+D N  K +    D   A+KLW
Sbjct: 249 LINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLW 306

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy Swiss-Prot
Match: Q8BYK4 (Retinol dehydrogenase 12 OS=Mus musculus OX=10090 GN=Rdh12 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.8e-26
Identity = 86/267 (32.21%), Postives = 127/267 (47.57%), Query Frame = 0


Query: 39  IITGATSGIGEETARVLAKRGMRIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSL 98
           +ITGA +GIG+ETAR LA+RG R+ +A RD+ K       I+ +   +++LV ++DLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIRADTKNSQVLVRKLDLSDT 102

Query: 99  ASVRRFCMEFAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKL 158
            S+R F   F A    L+ILINN GV       + D  E  F  N+LGH+++T LLL++L
Sbjct: 103 KSIRAFAERFLAEEKKLHILINNAGVMMCPYSKTTDGFETHFGVNHLGHFLLTYLLLERL 162

Query: 159 IETSKRTGIQGRIINVSSVVHCW-------VQWHACLCSI-------KTGLHFT------ 218
            E++       R++N+SS+ H         +Q     CS           L FT      
Sbjct: 163 KESA-----PARVVNLSSIAHLIGKIRFHDLQGQKRYCSAFAYGHSKLANLLFTRELAKR 222

Query: 219 CQGDS------------------------LFFMASKLLKSIPQGAATTCYVALSPQTRRK 262
            QG                          L+ + S   KS  QGA T+ + AL+      
Sbjct: 223 LQGTGVTAYAVHPGVVLSEITRNSYLLCLLWRLFSPFFKSTSQGAQTSLHCALAEDLEPL 282

[Close]

BLAST of Cp4.1LG20g02610 vs. NCBI nr
Match: XP_023519347.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476 bits (1226), Expect = 1.81e-167
Identity = 262/327 (80.12%), Postives = 267/327 (81.65%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM
Sbjct: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240

Query: 241 TGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 276
           T +    +G   DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS
Sbjct: 241 TAILKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 300

[Close]

BLAST of Cp4.1LG20g02610 vs. NCBI nr
Match: XP_023000696.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 463 bits (1191), Expect = 6.14e-162
Identity = 253/327 (77.37%), Postives = 265/327 (81.04%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKV+ERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSDDKLELTFATNYLGHY++TELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 276
           T +    +G   DSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTGRYFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

[Close]

BLAST of Cp4.1LG20g02610 vs. NCBI nr
Match: XP_022924073.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 459 bits (1180), Expect = 2.90e-160
Identity = 251/327 (76.76%), Postives = 263/327 (80.43%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVG+AGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKV+ERIKR GPEAEIL+SEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSDDKLELTFATNYLGHY++TELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 276
           T +    +G   DSLFFMASKLLKSIPQGAATTCYVALSPQT RKTGRYFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

[Close]

BLAST of Cp4.1LG20g02610 vs. NCBI nr
Match: KAG7019803.1 (Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443 bits (1139), Expect = 1.44e-153
Identity = 251/370 (67.84%), Postives = 261/370 (70.54%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVG+AGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 1   MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 60

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKV+ERIKR GPEAEIL+SEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSDDKLELTFATNYLGHY++TELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 180

Query: 181 WVQWH------------------------ACLCSIK------------------------ 240
           WV+                          AC+   K                        
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKVILLLQLLRFLQTVDKT 240

Query: 241 -------------------TGLH---------------------------FTCQGDSLFF 276
                                +H                            T   DSLFF
Sbjct: 241 VHILKFQPFLQERDANVTINAVHPGIPSSASSTSTKRKMYKRLLIYHNYILTSLADSLFF 300

[Close]

BLAST of Cp4.1LG20g02610 vs. NCBI nr
Match: XP_038895224.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 435 bits (1119), Expect = 3.51e-151
Identity = 240/326 (73.62%), Postives = 256/326 (78.53%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVGIAGPSGYGSK+SAE+VTE  Q SSP TAIITGATSGIGEETARVLAKRG+
Sbjct: 1   MKETLRYLVGIAGPSGYGSKASAEKVTESFQFSSPFTAIITGATSGIGEETARVLAKRGV 60

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKVKERIKRE PEAEILVSEIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKRESPEAEILVSEIDLSSLASVRRFCVEFAALGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYS+DK ELTFATNYLGHY++TELLLDKLIETSKRTGIQGRIINVSSVVH 
Sbjct: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHY 180

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 181 WVKREDLCFIHLLNPNKYNGTRAYAQSKLACILHAKEVARKLKEQDANVTINAVHPGIVK 240

Query: 241 TGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 275
           T +    +G   DSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTG+YFADCN+TKCSS
Sbjct: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGKYFADCNKTKCSS 300

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy TrEMBL
Match: A0A6J1KGJ5 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495058 PE=3 SV=1)

HSP 1 Score: 463 bits (1191), Expect = 2.97e-162
Identity = 253/327 (77.37%), Postives = 265/327 (81.04%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKV+ERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSDDKLELTFATNYLGHY++TELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 276
           T +    +G   DSLFFMASKLLKSIPQGAATTCYVALSPQT+RKTGRYFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy TrEMBL
Match: A0A6J1EBB9 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431615 PE=3 SV=1)

HSP 1 Score: 459 bits (1180), Expect = 1.40e-160
Identity = 251/327 (76.76%), Postives = 263/327 (80.43%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYLVG+AGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIVMAARDMKKAAKV+ERIKR GPEAEIL+SEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           NGGVFSPNLEYSDDKLELTFATNYLGHY++TELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 276
           T +    +G   DSLFFMASKLLKSIPQGAATTCYVALSPQT RKTGRYFADCNETKCSS
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy TrEMBL
Match: A0A6J1C5L3 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008680 PE=3 SV=1)

HSP 1 Score: 416 bits (1068), Expect = 1.05e-143
Identity = 228/325 (70.15%), Postives = 250/325 (76.92%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           MKETLRYL GIAG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRG+
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           RIV+AARD+KKAAKVKERIKRE  EAEILVSEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHY++TE+LLD LIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVQWH------------------------ACLCS------------------------IK 240
           WV+                          AC+                          +K
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 241 TGL---HFTCQGDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 274
           T +   H     DSLFFMASKLLKSIPQGAATTCYVAL+PQT RKTG+YF+DCNETKCS+
Sbjct: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy TrEMBL
Match: A0A6J1BYZ0 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006882 PE=3 SV=1)

HSP 1 Score: 359 bits (921), Expect = 4.26e-121
Identity = 201/331 (60.73%), Postives = 237/331 (71.60%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSS-----PLTAIITGATSGIGEETARVL 60
           MKETLRYL GIAGPSGYGS S+AEQVT++   SS     PLTAIITGATSGIG ETARVL
Sbjct: 15  MKETLRYLAGIAGPSGYGSNSTAEQVTQNYSISSSSTSHPLTAIITGATSGIGAETARVL 74

Query: 61  AKRGMRIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPL 120
           AKRG+RIVMAARD+KKAA+VKE I++E PEAEI+V EIDLSSLASV+RFC +F ALGLPL
Sbjct: 75  AKRGVRIVMAARDLKKAAQVKEGIQKETPEAEIIVCEIDLSSLASVQRFCNQFLALGLPL 134

Query: 121 NILINNGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVS 180
           NILINN GVFS NLE+S+DK+ELTFATNYLGHY +TE LL+K+IET+ +TGI+GRIINVS
Sbjct: 135 NILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAKTGIEGRIINVS 194

Query: 181 SVVHCWVQ-----------------------------WHACLCS---------------- 240
           SV+HCWV+                              HA   S                
Sbjct: 195 SVIHCWVKKDGLRFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQARNARVTINAVH 254

Query: 241 ---IKTGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNE 275
              +KT +    +G   DSLFFMASKLLK+  QGA+TTCYVALS QT  K+G+++ADCNE
Sbjct: 255 PGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNE 314

[Close]

BLAST of Cp4.1LG20g02610 vs. ExPASy TrEMBL
Match: A0A6P5Y9E7 (short-chain dehydrogenase TIC 32, chloroplastic OS=Durio zibethinus OX=66656 GN=LOC111289924 PE=3 SV=1)

HSP 1 Score: 353 bits (906), Expect = 1.20e-118
Identity = 193/330 (58.48%), Postives = 237/330 (71.82%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQC---SSPLTAIITGATSGIGEETARVLAK 60
           MKETLRYL GIAGPSGYGSKS+AEQV E+  C      LTAIITGATSGIG ETARVLAK
Sbjct: 28  MKETLRYLAGIAGPSGYGSKSTAEQVAENCSCLVDPQHLTAIITGATSGIGAETARVLAK 87

Query: 61  RGMRIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNI 120
           RG+R+V+ ARD+KKAA++KE I+RE P AEI++SEIDLSSLASV+RFC EF ALGLPLNI
Sbjct: 88  RGVRVVIPARDLKKAAELKEGIQRENPNAEIILSEIDLSSLASVKRFCTEFLALGLPLNI 147

Query: 121 LINNGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSV 180
           LINN G+FS NLE+S+DK+E+TFATNYLGHY++TE+LL+++IET+++TGIQGRIIN+SSV
Sbjct: 148 LINNAGIFSQNLEFSEDKIEMTFATNYLGHYLLTEMLLERMIETAEQTGIQGRIINLSSV 207

Query: 181 VHCWVQW-----------------------------------------HACLCS------ 240
           +H WV+                                          HA +        
Sbjct: 208 IHSWVKRECFSFTQMLNPNNYNGTRAYAQSKLANILHAKEIARQLKAKHAKVTINAVHPG 267

Query: 241 -IKTGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETK 276
            +KTG+    +G   DS+FF+ASKLLKS  QGA+T+CYVALSPQ +  +G+YFADCNET 
Sbjct: 268 IVKTGIIRAHKGFITDSIFFIASKLLKSTSQGASTSCYVALSPQAQGISGKYFADCNETN 327

[Close]

BLAST of Cp4.1LG20g02610 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-90
Identity = 187/328 (57.01%), Postives = 229/328 (69.82%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEH--LQCSSPLTAIITGATSGIGEETARVLAKR 60
           MK TLRYL GIAGP+G+GS+S+AEQVT+H    CS  LTAIITG TSGIG ETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSH-LTAIITGGTSGIGAETARVLAKR 60

Query: 61  GMRIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNIL 120
           G+R+VMA RDMKKA  VKERI RE PEA+I++ EIDLSSL+SV RFC +F +  LPLNIL
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 121 INNGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVV 180
           INN GVFSPNLE+S++K+ELTFATN+LGHY++TE+L++K+I+T++++GI+GRIIN+SSV+
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 181 HCWVQ------------------------------WHACLCS------------------ 240
           H WV+                               HA   S                  
Sbjct: 181 HNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPG 240

Query: 241 -IKTGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETK 275
            +KTG+    +G   DSLF +ASKLLKSI QGAATTCYVALS +T+  +G+YFADCNET 
Sbjct: 241 IVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNETN 300

[Close]

BLAST of Cp4.1LG20g02610 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 6.3e-69
Identity = 150/321 (46.73%), Postives = 200/321 (62.31%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           M ET+++L+G  GPSG+GS+S+A+ VT +    S LTAIITGATSGIG ETARVLAKRG 
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRS-LTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           R+V+ AR +K A + K RI  E P+AEI+V  +DLSSL SVRRF  +F +L LPLNILIN
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILIN 120

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           N G ++     S+D +E+TFATNYLGH+++T+LLL K+IET+ +TG+QGRI+NV+SVVH 
Sbjct: 121 NAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHS 180

Query: 181 W-----VQWHACLCS--------------------------------------------- 240
           W     +Q+ A +                                               
Sbjct: 181 WFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGI 240

Query: 241 IKTGLHFTCQG---DSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKC 269
           +KT L    +G   D +FF+ SKLLKS+PQ AATTCYVA SP+ R   G+YF+DCNE + 
Sbjct: 241 VKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEARS 300

[Close]

BLAST of Cp4.1LG20g02610 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 6.3e-69
Identity = 129/185 (69.73%), Postives = 162/185 (87.57%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEH--LQCSSPLTAIITGATSGIGEETARVLAKR 60
           MK TLRYL GIAGP+G+GS+S+AEQVT+H    CS  LTAIITG TSGIG ETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSH-LTAIITGGTSGIGAETARVLAKR 60

Query: 61  GMRIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNIL 120
           G+R+VMA RDMKKA  VKERI RE PEA+I++ EIDLSSL+SV RFC +F +  LPLNIL
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 121 INNGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVV 180
           INN GVFSPNLE+S++K+ELTFATN+LGHY++TE+L++K+I+T++++GI+GRIIN+SSV+
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 181 HCWVQ 184
           H WV+
Sbjct: 181 HNWVK 184

[Close]

BLAST of Cp4.1LG20g02610 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 3.5e-67
Identity = 146/321 (45.48%), Postives = 198/321 (61.68%), Query Frame = 0


Query: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60
           M ET +YL+G AG SG+GSKS+AE+VTE+    S +TA+ITGATSGIG ETARVLAKRG 
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRS-ITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120
           R++  AR++K A + KERI  E PE EI+V ++DLSS+ASVR F  +F +L LPLN+LIN
Sbjct: 61  RLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLIN 120

Query: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180
           N G  +     S+D +E+TFATNYLGH+++T LLL+K+I+T++ TG+QGRI+NV+S +H 
Sbjct: 121 NAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHG 180

Query: 181 W----------------VQWHAC-------LCSI-----------KTGLHFTCQ------ 240
           W                 Q+ A        L ++           K G + T        
Sbjct: 181 WFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGV 240

Query: 241 -------------GDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKC 269
                         D +FF+ASKL+K++PQ AATTCYVA +P+    +G+YF DCNET  
Sbjct: 241 VRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNETTP 300

[Close]

BLAST of Cp4.1LG20g02610 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 218.4 bits (555), Expect = 7.2e-57
Identity = 130/304 (42.76%), Postives = 179/304 (58.88%), Query Frame = 0


Query: 8   LVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGMRIVMAAR 67
           + G  GPSG+GS S+AE+VT+ +  ++ LTAIITG T GIG ETARVL+KRG  +V+ AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGIDATN-LTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  DMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 127
           +M  A   K  I R+   A + + ++DLSS+ S++ F  EF AL LPLN+LINN GV   
Sbjct: 67  NMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFC 126

Query: 128 NLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHCW-----V 187
             + S+D +EL FATN++GH+++T LLLD +  T+K +G++GRI+NVSSV H +     +
Sbjct: 127 PYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGI 186

Query: 188 QWHAC--LCSI-------------------------KTGLHFTCQG-------DSLF--- 247
           Q+ +   +CS                          + G++ T           +LF   
Sbjct: 187 QFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQHT 246

Query: 248 --------FMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSSLANDELAA 262
                   F +  L K+IPQGAATTCYVAL P  +  TG+YFADCNE   S LA DE  A
Sbjct: 247 ALLMRFLKFFSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETLA 306

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g02610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	1.1e-49	41.12	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	1.4e-49	41.12	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	4.1e-49	39.54	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	8.1e-45	38.46	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q8BYK4	3.8e-26	32.21	Retinol dehydrogenase 12 OS=Mus musculus OX=10090 GN=Rdh12 PE=2 SV=1	[more]

BLAST of Cp4.1LG20g02610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023519347.1	1.81e-167	80.12	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo...	[more]
XP_023000696.1	6.14e-162	77.37	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima]	[more]
XP_022924073.1	2.90e-160	76.76	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata]	[more]
KAG7019803.1	1.44e-153	67.84	Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_038895224.1	3.51e-151	73.62	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his...	[more]

BLAST of Cp4.1LG20g02610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KGJ5	2.97e-162	77.37	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EBB9	1.40e-160	76.76	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1C5L3	1.05e-143	70.15	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A6J1BYZ0	4.26e-121	60.73	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A6P5Y9E7	1.20e-118	58.48	short-chain dehydrogenase TIC 32, chloroplastic OS=Durio zibethinus OX=66656 GN=...	[more]

BLAST of Cp4.1LG20g02610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50130.1	1.3e-90	57.01	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	6.3e-69	46.73	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	6.3e-69	69.73	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24050.1	3.5e-67	45.48	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	7.2e-57	42.76	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 37..54 score: 44.95 coord: 163..179 score: 32.79 coord: 113..124 score: 39.38
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..178 e-value: 2.9E-27 score: 95.4
None	No IPR available	GENE3D	3.40.50.720		coord: 27..272 e-value: 1.2E-48 score: 167.7
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 199..271 coord: 4..184
None	No IPR available	PANTHER	PTHR24320:SF198	NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN	coord: 199..271 coord: 4..184
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g02610.1	Cp4.1LG20g02610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity