Cp4.1LG20g02180 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG20g02180
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionanaphase-promoting complex subunit 8
LocationCp4.1LG20: 1255450 .. 1259689 (-)
RNA-Seq ExpressionCp4.1LG20g02180
SyntenyCp4.1LG20g02180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGCGGGCAAGAGCCGGAGGCTCCAAGTCTGGTGGGGCGAAACGAACAGAAAGCGCATGTTAGCGACGTCCGAAATTTGAAGTTTTGAGTTAACAAGGAAGAGCGCAAGAAAGGCGCCATAGAGACGGAGATTCAATGAATTCAAAAGAAAACTGTAGAAACGAGCTTCGTACTGCAATCCGGCAGCTCAGTGATCGTTGTCTCTACTCTGCTTCCAAATGGTACAGTTCCCATAGCCCTAAAATTTCTCTCTCTCTCTCTTGTTGCTTCTGATCTTTATTTTATACTCAATCTTGTTTGAACTGCTCAAGTGCTGGTTGATGTGCTTAATTTGGATCTGGGGTGTCGAGTTCTCTTGAAGTTTAGATGTTAGTGTTACATGGTTTCTGAGAAAGCGGCGGACGAGGTCGAATTGGTGACTTTGAATCGTGACTTTGTTTACTTGATGAAGGCTCTACTTACACGCGATGTACTTTATGTATGATGAATTTTAAATTGCTGCGGTCGTAATTGATCGGAGTTCAAGTTTTGGTATCTAGTTCTTAAACAATCTGCAAGCTCTTGTAACAATTAATCTCCTCCGTAAATTGTAATATGACTAAAAGATTTAATTGAAGTTTTGAGATTCCTTGCCTCTACAGGGCAGCAGAGCAATTGGTGGGTATCGAGCAAGATCCAGCTAAGTTCACACCTTCAAACACCAGGTTTCAGCGTGGAAGTTCGAGTATTCGTCGGAGATTTCATACAAATGAGAGTTGTTCAACCCCTATTGCTGGCATATCTTATGTTTCTACTCCAGTGATGGAGGAGGATGAGGTTGTGGATGGTGATTTTTACCTTCTGGCCAAATCATACTTCGATTGCCGTGAGTACAAGAGGGCTGCTCATGTTCTTCGAGAGCAAAACGGAAAGAAATCTGTGTTCTTGCGGCAATATGCTCTTTATCTGGTCAGTTGATCCTTACCATCTATGATTTTTTTTTAGTTGCTATGATATCCAACAGTTTCTTAGTTCAGTTTACTAAAGAACTTAGAAATCAAGTGAAGGAATGTAAGTAGCTATAGCTTTCCAAACAAAAATAGTAAAAGAAAGATTTGTTACTCTCCTCTAATATCCTTCTGATTATATATGCTTGATCATGACTTGGATCATATCTATTGATTTCAATCAGAAAGTGATCTTGTTCATGTTATTTTTCATTTCAGACTTCACCTAGAATTTCAGCTTAAATGCAGTGCACATATTTAAATTGTTATTATGAGTATTCTTGATCATGTTAGCTAAGATCACGTGGAAATTTATCAGGTTCTTCCCAATTTGAGCTTTTTTTTTTCTTGTAGAAGAGCATTATTCTAAACCTGTCTGTGACTCATTGGACGTGTTCTTATTTTTCCATTGTCCAATGTCCATGAATTGTTAATAGTTAATAGTCATCAAGGAAAATGATGCATGAAACGTGAAAAATGTCTCTGACAACCTTTTCTATATAGATTCAGTACCATTTGTTTGATTCATAGACGTGAGACTGTTGAGGATTATTGGGAGTGAGTCCCACATTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGGCATACTATCTTCATCGGTATGAGGCTTTTTGGGGAAGTCCAAAGCAAACCCATGAGAGCCTATGCTCAAAATGGACAATATCATATCGTTGTGGAGAGTCGTGATTCCTAACATAGTGGTATCTGGAATAAGTTCCTCAAAAAACTTATTTGGTGCAACTGTAGTAAAGAAAATTTGAGAATAATAGGTGGAAAACGTAACATGTGAGATATGCTTTCTACACTAAACTATTTGCATACAATGCTTACTAATATCTGTTGTTCAGTCCAGTGGCTCAATTCATGTATTTCATTGATAAAAATGAAGAACAGTTAGAAACACAGTAGAACTCTCTTCAAAAAAAATTTGTAATAAACAGATTTCGTCTATGTTCTTGGTTATTTTTCACCTCGACTTTGTGGTTCTCTTTCTCAAGCCAGCAACTCACACTTTGCTTCGTGTAGGCCGGTGAAAAGCGAAAAGAAGAAGAGGTAGTAGAACTTGAGGGATCATTGGGTAAGAGCGATGCTGTAAATCAAGAACTGGTTTCATTGGAAAGGGAGTTATCAACCCTCCGAAAAAATAGCATGATCGACCCGTTTGGACTGTACTTGTATGGACTCGTGCTCAAACAAAAAGGCAGTGAAAATCTTGCTCGCACAGCTCTTATGGAGTCGGTGAACAGCTACCCTTGGAACTGGAGTGCATGGTCGGAGCTGCAATCCTTGTGCACTACAATTGACATATTGAACAGCCTTAATCTCAATAACCATTGGATGAAGGACTTCTTTCTTGCCTATGCTTACCAAGAACTCAGGATGCATAATGAATCCATGGTAAAATATGAGAACTTACAAGCTACTTTTGGTTTTAGTAATTACATACAAGCTCAAATTGCAAAAGCTCAATATAGCTTGAGGGAGTTTGATCAAGTTGAAGCAATATTTGAAGAACTTCTTAGGAACGATCCTTATCGGGTCGAAGACATGGATATGTATTCAAATGTCCTCTATGCAAAGGAATGCTTCTCTGCCTTGAGCTACCTTGCTCATAGAGTTTTCATGACAGATAAGTATAGACCTGAATCTTGCTGCATTATTGGAAATTACTATAGTTTGAAGGGGCAGCACGAGAAGTCAGTTGTATATTTTAGAAGGGCACTCAAATTAAACAAAAACTATTTATCTGCTTGGACTCTGATGGGACACGAATTTGTGGAGATGAAGAACATTCCTGCTGCCATTGATGCATACCGACGAGCTGTGGATATAAATTCGTGTGATTATCGAGCTTGGTATGGGTTAGGGCAAGCTTATGAAATGATGGGCATGCCTTTTTATGCTCTACATTACTTCAAGAAATCTGTATTTTTGCAACCGAACGATTCGAGATTGTGGATTGCCATGGCTCAGTGTTACGAAAGCGAACAGTTACGAATGCTGGAGGAGGCAATCAAGTGTTACAGAAGGGCTGCAAGTTGTAATGATAGAGAAGCCATTGCCCTGCACCAGTTGGCTAAACTGCATTCTGAGTTGGGGCAGTCTGAAGAGGCAGCATTCTACTACAAAAAGGATTTAGAAAGAATGGAAGCTGAAGAGAGGGAAGGACCCAATATGGTTGAAGCTTTGCTTTTTCTTGCTACCTACTATAAAGCACAAAAGAAATTTGATGAAGCAGAAGTGTATTGTACACGCCTTCTGGATTACACTGGTCCGGTGAGTATTATTGGTTTTGTGTGTTTGAAGTTTATTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAATGTGTGTTTGAAGTTTAGTCTTTCATGGCCAACATGTAGTTCAAGTGTCACTCAACCACCATTGTCTTTTTGGTGCTGTCATTCATTGCAGGAGAAAGAAACGGCTAAGGATTTACTCAGAGGAATGAGGCTACCACAGTCTAGCTTTCCATCTATGGATGTTGAGCTCTTCCCTCCCTAAGGTTTCTCCATTGCAACACCTGATTTTCTAACCAACTTTTCCAATATTCGGAAACGAATACAGGTCGGTCGATATCGAGCAATATATATACTGACTGCTAAGTGTTTGGCCTAGAAATCTTCATTTCTTTGAAGTTCCAACATCAAGGTTTGACGTCTTCACTACTCAATGTTTTTATTCAATTGTATTGCCCTTTCTCTCTCGCTCAGTCCACTCTAGGTTGGCTGTGCATTTTGAATTACCCAGTAGAAGGAGCATTTCTGTTGTATCTTTGTTGATTAATTCCAGTAGCCTGAATTTAGAAACCGTTCTAATTATTTCAAACCTTATTTTTTTGTTATCATTTCAATGTGTAATAGAAGTACCCAATTTATGCACTTTGGGAAAGAATGGTGCAAA

mRNA sequence

TGGGCGGGCAAGAGCCGGAGGCTCCAAGTCTGGTGGGGCGAAACGAACAGAAAGCGCATGTTAGCGACGTCCGAAATTTGAAGTTTTGAGTTAACAAGGAAGAGCGCAAGAAAGGCGCCATAGAGACGGAGATTCAATGAATTCAAAAGAAAACTGTAGAAACGAGCTTCGTACTGCAATCCGGCAGCTCAGTGATCGTTGTCTCTACTCTGCTTCCAAATGGGCAGCAGAGCAATTGGTGGGTATCGAGCAAGATCCAGCTAAGTTCACACCTTCAAACACCAGGTTTCAGCGTGGAAGTTCGAGTATTCGTCGGAGATTTCATACAAATGAGAGTTGTTCAACCCCTATTGCTGGCATATCTTATGTTTCTACTCCAGTGATGGAGGAGGATGAGGTTGTGGATGGTGATTTTTACCTTCTGGCCAAATCATACTTCGATTGCCGTGAGTACAAGAGGGCTGCTCATGTTCTTCGAGAGCAAAACGGAAAGAAATCTGTGTTCTTGCGGCAATATGCTCTTTATCTGGCCGGTGAAAAGCGAAAAGAAGAAGAGGTAGTAGAACTTGAGGGATCATTGGGTAAGAGCGATGCTGTAAATCAAGAACTGGTTTCATTGGAAAGGGAGTTATCAACCCTCCGAAAAAATAGCATGATCGACCCGTTTGGACTGTACTTGTATGGACTCGTGCTCAAACAAAAAGGCAGTGAAAATCTTGCTCGCACAGCTCTTATGGAGTCGGTGAACAGCTACCCTTGGAACTGGAGTGCATGGTCGGAGCTGCAATCCTTGTGCACTACAATTGACATATTGAACAGCCTTAATCTCAATAACCATTGGATGAAGGACTTCTTTCTTGCCTATGCTTACCAAGAACTCAGGATGCATAATGAATCCATGGTAAAATATGAGAACTTACAAGCTACTTTTGGTTTTAGTAATTACATACAAGCTCAAATTGCAAAAGCTCAATATAGCTTGAGGGAGTTTGATCAAGTTGAAGCAATATTTGAAGAACTTCTTAGGAACGATCCTTATCGGGTCGAAGACATGGATATGTATTCAAATGTCCTCTATGCAAAGGAATGCTTCTCTGCCTTGAGCTACCTTGCTCATAGAGTTTTCATGACAGATAAGTATAGACCTGAATCTTGCTGCATTATTGGAAATTACTATAGTTTGAAGGGGCAGCACGAGAAGTCAGTTGTATATTTTAGAAGGGCACTCAAATTAAACAAAAACTATTTATCTGCTTGGACTCTGATGGGACACGAATTTGTGGAGATGAAGAACATTCCTGCTGCCATTGATGCATACCGACGAGCTGTGGATATAAATTCGTGTGATTATCGAGCTTGGTATGGGTTAGGGCAAGCTTATGAAATGATGGGCATGCCTTTTTATGCTCTACATTACTTCAAGAAATCTGTATTTTTGCAACCGAACGATTCGAGATTGTGGATTGCCATGGCTCAGTGTTACGAAAGCGAACAGTTACGAATGCTGGAGGAGGCAATCAAGTGTTACAGAAGGGCTGCAAGTTGTAATGATAGAGAAGCCATTGCCCTGCACCAGTTGGCTAAACTGCATTCTGAGTTGGGGCAGTCTGAAGAGGCAGCATTCTACTACAAAAAGGATTTAGAAAGAATGGAAGCTGAAGAGAGGGAAGGACCCAATATGGTTGAAGCTTTGCTTTTTCTTGCTACCTACTATAAAGCACAAAAGAAATTTGATGAAGCAGAAGTGTATTGTACACGCCTTCTGGATTACACTGGTCCGGAGAAAGAAACGGCTAAGGATTTACTCAGAGGAATGAGGCTACCACAGTCTAGCTTTCCATCTATGGATGTTGAGCTCTTCCCTCCCTAAGGTTTCTCCATTGCAACACCTGATTTTCTAACCAACTTTTCCAATATTCGGAAACGAATACAGGTCGGTCGATATCGAGCAATATATATACTGACTGCTAAGTGTTTGGCCTAGAAATCTTCATTTCTTTGAAGTTCCAACATCAAGGTTTGACGTCTTCACTACTCAATGTTTTTATTCAATTGTATTGCCCTTTCTCTCTCGCTCAGTCCACTCTAGGTTGGCTGTGCATTTTGAATTACCCAGTAGAAGGAGCATTTCTGTTGTATCTTTGTTGATTAATTCCAGTAGCCTGAATTTAGAAACCGTTCTAATTATTTCAAACCTTATTTTTTTGTTATCATTTCAATGTGTAATAGAAGTACCCAATTTATGCACTTTGGGAAAGAATGGTGCAAA

Coding sequence (CDS)

ATGAATTCAAAAGAAAACTGTAGAAACGAGCTTCGTACTGCAATCCGGCAGCTCAGTGATCGTTGTCTCTACTCTGCTTCCAAATGGGCAGCAGAGCAATTGGTGGGTATCGAGCAAGATCCAGCTAAGTTCACACCTTCAAACACCAGGTTTCAGCGTGGAAGTTCGAGTATTCGTCGGAGATTTCATACAAATGAGAGTTGTTCAACCCCTATTGCTGGCATATCTTATGTTTCTACTCCAGTGATGGAGGAGGATGAGGTTGTGGATGGTGATTTTTACCTTCTGGCCAAATCATACTTCGATTGCCGTGAGTACAAGAGGGCTGCTCATGTTCTTCGAGAGCAAAACGGAAAGAAATCTGTGTTCTTGCGGCAATATGCTCTTTATCTGGCCGGTGAAAAGCGAAAAGAAGAAGAGGTAGTAGAACTTGAGGGATCATTGGGTAAGAGCGATGCTGTAAATCAAGAACTGGTTTCATTGGAAAGGGAGTTATCAACCCTCCGAAAAAATAGCATGATCGACCCGTTTGGACTGTACTTGTATGGACTCGTGCTCAAACAAAAAGGCAGTGAAAATCTTGCTCGCACAGCTCTTATGGAGTCGGTGAACAGCTACCCTTGGAACTGGAGTGCATGGTCGGAGCTGCAATCCTTGTGCACTACAATTGACATATTGAACAGCCTTAATCTCAATAACCATTGGATGAAGGACTTCTTTCTTGCCTATGCTTACCAAGAACTCAGGATGCATAATGAATCCATGGTAAAATATGAGAACTTACAAGCTACTTTTGGTTTTAGTAATTACATACAAGCTCAAATTGCAAAAGCTCAATATAGCTTGAGGGAGTTTGATCAAGTTGAAGCAATATTTGAAGAACTTCTTAGGAACGATCCTTATCGGGTCGAAGACATGGATATGTATTCAAATGTCCTCTATGCAAAGGAATGCTTCTCTGCCTTGAGCTACCTTGCTCATAGAGTTTTCATGACAGATAAGTATAGACCTGAATCTTGCTGCATTATTGGAAATTACTATAGTTTGAAGGGGCAGCACGAGAAGTCAGTTGTATATTTTAGAAGGGCACTCAAATTAAACAAAAACTATTTATCTGCTTGGACTCTGATGGGACACGAATTTGTGGAGATGAAGAACATTCCTGCTGCCATTGATGCATACCGACGAGCTGTGGATATAAATTCGTGTGATTATCGAGCTTGGTATGGGTTAGGGCAAGCTTATGAAATGATGGGCATGCCTTTTTATGCTCTACATTACTTCAAGAAATCTGTATTTTTGCAACCGAACGATTCGAGATTGTGGATTGCCATGGCTCAGTGTTACGAAAGCGAACAGTTACGAATGCTGGAGGAGGCAATCAAGTGTTACAGAAGGGCTGCAAGTTGTAATGATAGAGAAGCCATTGCCCTGCACCAGTTGGCTAAACTGCATTCTGAGTTGGGGCAGTCTGAAGAGGCAGCATTCTACTACAAAAAGGATTTAGAAAGAATGGAAGCTGAAGAGAGGGAAGGACCCAATATGGTTGAAGCTTTGCTTTTTCTTGCTACCTACTATAAAGCACAAAAGAAATTTGATGAAGCAGAAGTGTATTGTACACGCCTTCTGGATTACACTGGTCCGGAGAAAGAAACGGCTAAGGATTTACTCAGAGGAATGAGGCTACCACAGTCTAGCTTTCCATCTATGGATGTTGAGCTCTTCCCTCCCTAA

Protein sequence

MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKKSVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLYLYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Homology
BLAST of Cp4.1LG20g02180 vs. ExPASy Swiss-Prot
Match: Q9STS3 (Anaphase-promoting complex subunit 8 OS=Arabidopsis thaliana OX=3702 GN=APC8 PE=1 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 7.3e-274
Identity = 457/578 (79.07%), Postives = 518/578 (89.62%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           M SKE CRNE+R AIRQLSDRCLYSA+KWA EQLVGIEQDP+ FTP+NTRFQRGSSSIRR
Sbjct: 1   MVSKECCRNEIRAAIRQLSDRCLYSAAKWAGEQLVGIEQDPSNFTPANTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPI--AGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNG 120
           RF TNES STP+   G S  +TP+ EEDE +DGD YLLAKSYFDCREY+RA+H+LR+Q  
Sbjct: 61  RFSTNESISTPLPSVGFSQAATPLPEEDEAIDGDIYLLAKSYFDCREYRRASHMLRDQVS 120

Query: 121 KKSVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFG 180
           KKS+FLR YALYLAGEKRKEEE++ELEG LGKSDA+N+ELVSLER+LS LR+   ID FG
Sbjct: 121 KKSLFLRYYALYLAGEKRKEEEMIELEGPLGKSDAINRELVSLERDLSALRRTGAIDSFG 180

Query: 181 LYLYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKD 240
           LYLYG+VLK+KG+E+LAR +L+ESVNSYPWNWSAWSELQSLCT+I+ILNSLNLNNHWMK+
Sbjct: 181 LYLYGVVLKEKGNESLARASLVESVNSYPWNWSAWSELQSLCTSIEILNSLNLNNHWMKE 240

Query: 241 FFLAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRN 300
           FFL  AYQELRMH ES+ KYE LQ  F FSNYIQAQ AKAQYSLREFDQVE +FEELLRN
Sbjct: 241 FFLGNAYQELRMHTESLAKYEYLQGIFSFSNYIQAQTAKAQYSLREFDQVEIMFEELLRN 300

Query: 301 DPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVV 360
           DPYRVEDMD+YSNVLYAKE  +ALSYLAH+VF+TDKYRPESCCIIGNYYSLKGQHEK+V+
Sbjct: 301 DPYRVEDMDLYSNVLYAKEACAALSYLAHKVFLTDKYRPESCCIIGNYYSLKGQHEKAVM 360

Query: 361 YFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMM 420
           YFRRALKLNK YLSAWTLMGHE+VEMKN PAAIDAYRRAVDIN  DYRAWYGLGQAYEMM
Sbjct: 361 YFRRALKLNKKYLSAWTLMGHEYVEMKNTPAAIDAYRRAVDINPTDYRAWYGLGQAYEMM 420

Query: 421 GMPFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIAL 480
           GMPFYALHYF+KS+F  PNDSRLWIAMA+CY++EQL MLEEAIKCY+RA +C D E IAL
Sbjct: 421 GMPFYALHYFRKSIFFLPNDSRLWIAMAKCYQTEQLYMLEEAIKCYKRAVNCTDTEGIAL 480

Query: 481 HQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVY 540
           +QLAKLH +LG++EEAA+Y++KDLERM+AE  EGPNM EAL+FLAT++K  KKF+EAEVY
Sbjct: 481 NQLAKLHQKLGRNEEAAYYFEKDLERMDAEGLEGPNMFEALVFLATHFKNHKKFEEAEVY 540

Query: 541 CTRLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFP 577
           CTRLLDY+GPEKE AK LLRG+R+ Q+ FPSMD+E FP
Sbjct: 541 CTRLLDYSGPEKEKAKSLLRGIRMAQTGFPSMDLEHFP 578

BLAST of Cp4.1LG20g02180 vs. ExPASy Swiss-Prot
Match: Q86B11 (Anaphase-promoting complex subunit 8 OS=Dictyostelium discoideum OX=44689 GN=anapc8 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 9.3e-128
Identity = 261/598 (43.65%), Postives = 371/598 (62.04%), Query Frame = 0

Query: 10  ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRRRFHTNESCS 69
           EL  +I  L+ R L  +SKW++EQL G+                 S +I     TNE   
Sbjct: 7   ELIKSINDLNSRGLLLSSKWSSEQLNGL-----------------SPTILATPLTNEE-- 66

Query: 70  TPIAGISYVSTPVMEEDEVVDGDF--YLLAKSYFDCREYKRAAHVLREQNG-KKSVFLRQ 129
                +S +S P +    +   ++  Y+LAK+YFD +EY+R + VL + N  +  +FLR 
Sbjct: 67  ----QLSIISQPSISSPPIGSNEYYKYILAKNYFDLKEYRRCSDVLIDCNKYQLPIFLRS 126

Query: 130 YALYLAGEKRKEEEVVELE------------------------------GSLGKSDAVNQ 189
           YA YLA EKR+EE+++E +                               +  K+D   Q
Sbjct: 127 YATYLAIEKRREEDIIEQQAQQQQQQQQAQQQAQQAQQESQQNDKNNDTNNNNKTDQQQQ 186

Query: 190 --------------ELVSLERELSTLRKNSMIDPFGLYLYGLVLKQKGSENLARTALMES 249
                         +L    ++L    K  M D F LY Y ++LK++    +AR  L+ES
Sbjct: 187 QQQQQKIEQCQEFKQLFQFYKKLYIENKKDM-DGFLLYFYSMLLKKQRDFTMARKVLIES 246

Query: 250 VNSYPWNWSAWSELQSLCT-TIDILNSLNLNNHWMKDFFLAYAYQELRMHNESMVKYENL 309
           V+ YP NWSAWS+L SLC+ + DI+  L+L +H+MKDFFLA+   EL+ +NES+V Y+ L
Sbjct: 247 VHKYPCNWSAWSDLSSLCSDSADIIMQLSLPDHFMKDFFLAHFKLELQQNNESLVIYQQL 306

Query: 310 QAT-FGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFS 369
             T F  S YI AQ A   Y+LR +D  E +FE L+  +P R+E++D+YSN+LY ++  +
Sbjct: 307 SRTLFTQSTYILAQTAIGNYNLRAYDIGEELFERLIELEPNRLENIDIYSNILYVRDKKA 366

Query: 370 ALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHE 429
           +LS LAH+    +KY PE+CCIIGNYYSLK +H+K+++YF+RALKLN  YLSAWTL+GHE
Sbjct: 367 SLSMLAHKAMKIEKYCPETCCIIGNYYSLKLEHDKAILYFQRALKLNDRYLSAWTLIGHE 426

Query: 430 FVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDSR 489
           F+E+KN+ AAI+AYR+AVDIN  DYRAWYGLGQ Y+++ +P Y+L+YFKK+  L+P D R
Sbjct: 427 FLEIKNVSAAINAYRKAVDINPRDYRAWYGLGQTYQLLKLPLYSLYYFKKATTLRPYDPR 486

Query: 490 LWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYKK 549
           +W A   CYE   +  + EAIKCY RA    DRE +A+++LAKL+ E+  +E+AAFYYKK
Sbjct: 487 MWCAAGGCYEF--IERIPEAIKCYERAEENYDRERVAINKLAKLYQEIQNNEKAAFYYKK 546

Query: 550 DLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLLR 559
           +L   + E+ +G  +++ALLFLA +YK Q +  ++E YC RLLDY GPEKE AK +LR
Sbjct: 547 NLYYCDQEKIDGQEIIDALLFLANFYKNQNQ-TQSEQYCLRLLDYAGPEKEEAKSILR 577

BLAST of Cp4.1LG20g02180 vs. ExPASy Swiss-Prot
Match: A1A4R8 (Cell division cycle protein 23 homolog OS=Bos taurus OX=9913 GN=CDC23 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 3.0e-118
Identity = 229/481 (47.61%), Postives = 316/481 (65.70%), Query Frame = 0

Query: 81  PVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKKSVFLRQYALYLAGEKRKEEE 140
           P + E++  D D Y LAK+YFD +EY RAAH L   N KK+ FL  Y+ YL+GEK+K++E
Sbjct: 74  PPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDE 133

Query: 141 VVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLYLYGLVLKQKGSENLARTALM 200
            V+  G L K    N+ L  L  ELS   +   +D FGLYLYG+VL++      A    +
Sbjct: 134 TVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFV 193

Query: 201 ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAYAYQELRMHNESMVKYEN 260
           E+ +  P +W AW EL +L T  ++L  L+L + WMK+FFLA+ Y EL++  E++ KY+N
Sbjct: 194 EATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQN 253

Query: 261 L-QATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECF 320
           L    F  S+YI +QIA A +++R+ D+  +IF EL + DPYR+E+MD +SN+LY +   
Sbjct: 254 LIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMK 313

Query: 321 SALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGH 380
           S LSYLAH +   DKYR E+CC+IGNYYSL+ QHEK+ +YF+RALKLN  YL AWTLMGH
Sbjct: 314 SELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGH 373

Query: 381 EFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDS 440
           E++EMKN  AAI AYR A+++N  DYRAWYGLGQ YE++ MPFY L+Y++++  L+PNDS
Sbjct: 374 EYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDS 433

Query: 441 RLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYK 500
           R+ +A+ +CY  E+L  L EA KCY RA +  D E +AL +LAKLH +L +SE+AA  Y 
Sbjct: 434 RMLVALGECY--EKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYI 493

Query: 501 KDLERMEA--EEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLL 559
           K ++ + +  E  E      A  +LA YY   K +DEA   C +        +E  K LL
Sbjct: 494 KYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASA-CAQKCCAFNDTREEGKALL 551

BLAST of Cp4.1LG20g02180 vs. ExPASy Swiss-Prot
Match: Q9UJX2 (Cell division cycle protein 23 homolog OS=Homo sapiens OX=9606 GN=CDC23 PE=1 SV=3)

HSP 1 Score: 427.2 bits (1097), Expect = 3.0e-118
Identity = 229/481 (47.61%), Postives = 316/481 (65.70%), Query Frame = 0

Query: 81  PVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKKSVFLRQYALYLAGEKRKEEE 140
           P + E++  D D Y LAK+YFD +EY RAAH L   N KK+ FL  Y+ YL+GEK+K++E
Sbjct: 74  PPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDE 133

Query: 141 VVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLYLYGLVLKQKGSENLARTALM 200
            V+  G L K    N+ L  L  ELS   +   +D FGLYLYG+VL++      A    +
Sbjct: 134 TVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFV 193

Query: 201 ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAYAYQELRMHNESMVKYEN 260
           E+ +  P +W AW EL +L T  ++L  L+L + WMK+FFLA+ Y EL++  E++ KY+N
Sbjct: 194 EATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQN 253

Query: 261 L-QATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECF 320
           L    F  S+YI +QIA A +++R+ D+  +IF EL + DPYR+E+MD +SN+LY +   
Sbjct: 254 LIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMK 313

Query: 321 SALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGH 380
           S LSYLAH +   DKYR E+CC+IGNYYSL+ QHEK+ +YF+RALKLN  YL AWTLMGH
Sbjct: 314 SELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGH 373

Query: 381 EFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDS 440
           E++EMKN  AAI AYR A+++N  DYRAWYGLGQ YE++ MPFY L+Y++++  L+PNDS
Sbjct: 374 EYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDS 433

Query: 441 RLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYK 500
           R+ +A+ +CY  E+L  L EA KCY RA +  D E +AL +LAKLH +L +SE+AA  Y 
Sbjct: 434 RMLVALGECY--EKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYI 493

Query: 501 KDLERMEA--EEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLL 559
           K ++ + +  E  E      A  +LA YY   K +DEA   C +        +E  K LL
Sbjct: 494 KYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEAST-CAQKCCAFNDTREEGKALL 551

BLAST of Cp4.1LG20g02180 vs. ExPASy Swiss-Prot
Match: Q8BGZ4 (Cell division cycle protein 23 homolog OS=Mus musculus OX=10090 GN=Cdc23 PE=1 SV=2)

HSP 1 Score: 424.5 bits (1090), Expect = 2.0e-117
Identity = 228/481 (47.40%), Postives = 315/481 (65.49%), Query Frame = 0

Query: 81  PVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKKSVFLRQYALYLAGEKRKEEE 140
           P + E++  D D Y LAK+YFD +EY RAAH L   N KK+ FL  Y+ YL+GEK+K++E
Sbjct: 74  PPLTEEDAQDVDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDE 133

Query: 141 VVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLYLYGLVLKQKGSENLARTALM 200
            V+  G L K    N+ L  L  ELS   +   +D FGLYLYG+VL++      A    +
Sbjct: 134 TVDSLGPLEKGQVKNEALRELRVELSRKHQARGLDGFGLYLYGVVLRKLDLVKEAIDVFV 193

Query: 201 ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFFLAYAYQELRMHNESMVKYEN 260
           E+ +  P +W AW EL +L T  ++L  L+L + WMK+FFLA+ Y EL++  E++ KY++
Sbjct: 194 EATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQH 253

Query: 261 L-QATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECF 320
           L    F  S+YI +QIA A +++R+ D+  +IF EL + DPYR+E+MD +SN+LY +   
Sbjct: 254 LIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMK 313

Query: 321 SALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGH 380
           S LSYLAH +   DKYR E+CC+IGNYYSL+ QHEK+ +YF+RALKLN  YL AWTLMGH
Sbjct: 314 SELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGH 373

Query: 381 EFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDS 440
           E++EMKN  AAI AYR A+++N  DYRAWYGLGQ YE++ MPFY L+Y++++  L+PNDS
Sbjct: 374 EYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDS 433

Query: 441 RLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYK 500
           R+ +A+ +CY  E+L  L EA KCY RA +  D E  AL +LAKLH +L +SE+AA  Y 
Sbjct: 434 RMLVALGECY--EKLNQLVEAKKCYWRAYAVGDVEKKALVKLAKLHEQLTESEQAAQCYI 493

Query: 501 KDLERMEA--EEREGPNMVEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETAKDLL 559
           K ++ + +  E  E      A  +LA YY   K +DEA   C +        +E  K LL
Sbjct: 494 KYIQDIYSCGETVEHLEESTAFRYLAQYYFKCKLWDEAST-CAQKCCAFNDTREEGKALL 551

BLAST of Cp4.1LG20g02180 vs. NCBI nr
Match: XP_023519242.1 (anaphase-promoting complex subunit 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1157 bits (2993), Expect = 0.0
Identity = 577/577 (100.00%), Postives = 577/577 (100.00%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. NCBI nr
Match: KAG6584242.1 (Anaphase-promoting complex subunit 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019838.1 Anaphase-promoting complex subunit 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 573/577 (99.31%), Postives = 574/577 (99.48%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRK  MIDPFGLY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKKGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LAYAYQELRMHNES+VKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYQELRMHNESLVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSEL QSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELRQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. NCBI nr
Match: XP_022924091.1 (anaphase-promoting complex subunit 8 [Cucurbita moschata])

HSP 1 Score: 1146 bits (2965), Expect = 0.0
Identity = 571/577 (98.96%), Postives = 573/577 (99.31%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRK  MIDPFGLY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKKGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTALMESVNSYPWNWSAW ELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWLELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LAYAYQELRMHNES+VKYENLQATFGFSNYIQAQ+AKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYQELRMHNESLVKYENLQATFGFSNYIQAQVAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSEL QSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELRQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. NCBI nr
Match: XP_023000742.1 (anaphase-promoting complex subunit 8 [Cucurbita maxima])

HSP 1 Score: 1144 bits (2958), Expect = 0.0
Identity = 570/577 (98.79%), Postives = 573/577 (99.31%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLRQYALYLAGEKRKEEEVVELEGSLGKS+AVNQELVSLERELSTLRKN MIDPFGLY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSNAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSE+LARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSESLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LAYAY ELRMHNES+VKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYHELRMHNESLVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDIN CDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINPCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSEL QSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELRQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. NCBI nr
Match: XP_004152456.1 (anaphase-promoting complex subunit 8 [Cucumis sativus] >KGN64281.1 hypothetical protein Csa_014254 [Cucumis sativus])

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 557/577 (96.53%), Postives = 568/577 (98.44%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           M SK+NCR+ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MTSKDNCRHELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFH+NE  STPIAG+SYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHSNEGSSTPIAGMSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLR YALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKN MIDPFGLY
Sbjct: 121 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTAL+ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LA AYQELRMHNES+VKYENLQ TF FSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LASAYQELRMHNESLVKYENLQGTFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLE+AIKCYRRAA+CNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEDAIKCYRRAANCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAE+YCT
Sbjct: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEIYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAK+LLRGMR+ QSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKNLLRGMRIAQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. ExPASy TrEMBL
Match: A0A6J1E8K9 (anaphase-promoting complex subunit 8 OS=Cucurbita moschata OX=3662 GN=LOC111431629 PE=4 SV=1)

HSP 1 Score: 1146 bits (2965), Expect = 0.0
Identity = 571/577 (98.96%), Postives = 573/577 (99.31%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRK  MIDPFGLY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKKGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTALMESVNSYPWNWSAW ELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWLELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LAYAYQELRMHNES+VKYENLQATFGFSNYIQAQ+AKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYQELRMHNESLVKYENLQATFGFSNYIQAQVAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSEL QSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELRQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. ExPASy TrEMBL
Match: A0A6J1KEI3 (anaphase-promoting complex subunit 8 OS=Cucurbita maxima OX=3661 GN=LOC111495101 PE=4 SV=1)

HSP 1 Score: 1144 bits (2958), Expect = 0.0
Identity = 570/577 (98.79%), Postives = 573/577 (99.31%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLRQYALYLAGEKRKEEEVVELEGSLGKS+AVNQELVSLERELSTLRKN MIDPFGLY
Sbjct: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSNAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSE+LARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSESLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LAYAY ELRMHNES+VKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LAYAYHELRMHNESLVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDIN CDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINPCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSEL QSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 481 LAKLHSELRQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. ExPASy TrEMBL
Match: A0A0A0LW67 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045790 PE=4 SV=1)

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 557/577 (96.53%), Postives = 568/577 (98.44%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           M SK+NCR+ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MTSKDNCRHELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFH+NE  STPIAG+SYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHSNEGSSTPIAGMSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLR YALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKN MIDPFGLY
Sbjct: 121 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTAL+ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LA AYQELRMHNES+VKYENLQ TF FSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LASAYQELRMHNESLVKYENLQGTFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLE+AIKCYRRAA+CNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEDAIKCYRRAANCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAE+YCT
Sbjct: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEIYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAK+LLRGMR+ QSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKNLLRGMRIAQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. ExPASy TrEMBL
Match: A0A1S3AVU0 (anaphase-promoting complex subunit 8 OS=Cucumis melo OX=3656 GN=LOC103483216 PE=4 SV=1)

HSP 1 Score: 1121 bits (2899), Expect = 0.0
Identity = 558/577 (96.71%), Postives = 567/577 (98.27%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           M SK+NCR ELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 1   MTSKDNCRYELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFH+NE  STPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK
Sbjct: 61  RFHSNEGSSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLR YALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKN MIDPFGLY
Sbjct: 121 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNGMIDPFGLY 180

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLARTAL+ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 181 LYGLVLKQKGSENLARTALVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LA AYQELRMHNES+VKYENLQ TF FSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 241 LASAYQELRMHNESLVKYENLQGTFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLE+AIKCYRRAA+CNDREAIALHQ
Sbjct: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEDAIKCYRRAANCNDREAIALHQ 480

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAE+YCT
Sbjct: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEIYCT 540

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAK+LLRGMR+ QSSFPSMDVELFPP
Sbjct: 541 RLLDYTGPEKETAKNLLRGMRIAQSSFPSMDVELFPP 577

BLAST of Cp4.1LG20g02180 vs. ExPASy TrEMBL
Match: A0A6J1K5K0 (anaphase-promoting complex subunit 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490258 PE=4 SV=1)

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 556/577 (96.36%), Postives = 564/577 (97.75%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           M+SKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR
Sbjct: 36  MSSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 95

Query: 61  RFHTNESCSTPIAGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNGKK 120
           RFHTNE CSTPI G+SYVSTPVMEEDEVVDGDFYLLAKSYFDCREY RAAHVLREQNGKK
Sbjct: 96  RFHTNEGCSTPITGLSYVSTPVMEEDEVVDGDFYLLAKSYFDCREYNRAAHVLREQNGKK 155

Query: 121 SVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFGLY 180
           SVFLR YALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKN   DPF LY
Sbjct: 156 SVFLRLYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNGTADPFELY 215

Query: 181 LYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 240
           LYGLVLKQKGSENLART L+ESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF
Sbjct: 216 LYGLVLKQKGSENLARTVLVESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKDFF 275

Query: 241 LAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 300
           LA AYQELRMHNES+ KYENLQATF FSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP
Sbjct: 276 LASAYQELRMHNESLAKYENLQATFSFSNYIQAQIAKAQYSLREFDQVEAIFEELLRNDP 335

Query: 301 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 360
           YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF
Sbjct: 336 YRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVVYF 395

Query: 361 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 420
           RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM
Sbjct: 396 RRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMMGM 455

Query: 421 PFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIALHQ 480
           PFYALHYFKK+VFLQPNDSR+WIAMAQC+ESEQLRMLEEAIKCYRRAA+CNDREAIALHQ
Sbjct: 456 PFYALHYFKKAVFLQPNDSRVWIAMAQCFESEQLRMLEEAIKCYRRAANCNDREAIALHQ 515

Query: 481 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 540
           LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT
Sbjct: 516 LAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVYCT 575

Query: 541 RLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFPP 577
           RLLDYTGPEKETAKDLLRGMRL QSSFPSMDVELFPP
Sbjct: 576 RLLDYTGPEKETAKDLLRGMRLAQSSFPSMDVELFPP 612

BLAST of Cp4.1LG20g02180 vs. TAIR 10
Match: AT3G48150.1 (anaphase-promoting complex subunit 8 )

HSP 1 Score: 944.1 bits (2439), Expect = 5.2e-275
Identity = 457/578 (79.07%), Postives = 518/578 (89.62%), Query Frame = 0

Query: 1   MNSKENCRNELRTAIRQLSDRCLYSASKWAAEQLVGIEQDPAKFTPSNTRFQRGSSSIRR 60
           M SKE CRNE+R AIRQLSDRCLYSA+KWA EQLVGIEQDP+ FTP+NTRFQRGSSSIRR
Sbjct: 1   MVSKECCRNEIRAAIRQLSDRCLYSAAKWAGEQLVGIEQDPSNFTPANTRFQRGSSSIRR 60

Query: 61  RFHTNESCSTPI--AGISYVSTPVMEEDEVVDGDFYLLAKSYFDCREYKRAAHVLREQNG 120
           RF TNES STP+   G S  +TP+ EEDE +DGD YLLAKSYFDCREY+RA+H+LR+Q  
Sbjct: 61  RFSTNESISTPLPSVGFSQAATPLPEEDEAIDGDIYLLAKSYFDCREYRRASHMLRDQVS 120

Query: 121 KKSVFLRQYALYLAGEKRKEEEVVELEGSLGKSDAVNQELVSLERELSTLRKNSMIDPFG 180
           KKS+FLR YALYLAGEKRKEEE++ELEG LGKSDA+N+ELVSLER+LS LR+   ID FG
Sbjct: 121 KKSLFLRYYALYLAGEKRKEEEMIELEGPLGKSDAINRELVSLERDLSALRRTGAIDSFG 180

Query: 181 LYLYGLVLKQKGSENLARTALMESVNSYPWNWSAWSELQSLCTTIDILNSLNLNNHWMKD 240
           LYLYG+VLK+KG+E+LAR +L+ESVNSYPWNWSAWSELQSLCT+I+ILNSLNLNNHWMK+
Sbjct: 181 LYLYGVVLKEKGNESLARASLVESVNSYPWNWSAWSELQSLCTSIEILNSLNLNNHWMKE 240

Query: 241 FFLAYAYQELRMHNESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRN 300
           FFL  AYQELRMH ES+ KYE LQ  F FSNYIQAQ AKAQYSLREFDQVE +FEELLRN
Sbjct: 241 FFLGNAYQELRMHTESLAKYEYLQGIFSFSNYIQAQTAKAQYSLREFDQVEIMFEELLRN 300

Query: 301 DPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVV 360
           DPYRVEDMD+YSNVLYAKE  +ALSYLAH+VF+TDKYRPESCCIIGNYYSLKGQHEK+V+
Sbjct: 301 DPYRVEDMDLYSNVLYAKEACAALSYLAHKVFLTDKYRPESCCIIGNYYSLKGQHEKAVM 360

Query: 361 YFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMM 420
           YFRRALKLNK YLSAWTLMGHE+VEMKN PAAIDAYRRAVDIN  DYRAWYGLGQAYEMM
Sbjct: 361 YFRRALKLNKKYLSAWTLMGHEYVEMKNTPAAIDAYRRAVDINPTDYRAWYGLGQAYEMM 420

Query: 421 GMPFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLRMLEEAIKCYRRAASCNDREAIAL 480
           GMPFYALHYF+KS+F  PNDSRLWIAMA+CY++EQL MLEEAIKCY+RA +C D E IAL
Sbjct: 421 GMPFYALHYFRKSIFFLPNDSRLWIAMAKCYQTEQLYMLEEAIKCYKRAVNCTDTEGIAL 480

Query: 481 HQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEAEVY 540
           +QLAKLH +LG++EEAA+Y++KDLERM+AE  EGPNM EAL+FLAT++K  KKF+EAEVY
Sbjct: 481 NQLAKLHQKLGRNEEAAYYFEKDLERMDAEGLEGPNMFEALVFLATHFKNHKKFEEAEVY 540

Query: 541 CTRLLDYTGPEKETAKDLLRGMRLPQSSFPSMDVELFP 577
           CTRLLDY+GPEKE AK LLRG+R+ Q+ FPSMD+E FP
Sbjct: 541 CTRLLDYSGPEKEKAKSLLRGIRMAQTGFPSMDLEHFP 578

BLAST of Cp4.1LG20g02180 vs. TAIR 10
Match: AT2G20000.1 (CDC27 family protein )

HSP 1 Score: 136.0 bits (341), Expect = 9.9e-32
Identity = 92/338 (27.22%), Postives = 163/338 (48.22%), Query Frame = 0

Query: 216 LQSLCTTIDILNSLNLNNHWMKDFFLAYAYQELRMHNESMVKYENLQATFGFSNYIQAQI 275
           + S   TI +   LNL     +   L+Y Y+      E++  Y  L      + ++ +Q+
Sbjct: 401 MSSQTITIGVSEILNLLRTLGEGCRLSYMYR----CQEALDTYMKLPHKHYNTGWVLSQV 460

Query: 276 AKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKY 335
            KA + L ++ + E  F       PY +E MD+YS VLY  +    LSYLA  +  TD+ 
Sbjct: 461 GKAYFELIDYLEAEKAFRLARLASPYCLEGMDIYSTVLYHLKEDMKLSYLAQELISTDRL 520

Query: 336 RPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYR 395
            P+S C +GN YSL+  HE ++  F RA++LN  +  A TL GHE+  +++    + +Y+
Sbjct: 521 APQSWCAMGNCYSLQKDHETALKNFLRAVQLNPRFAYAHTLCGHEYTTLEDFENGMKSYQ 580

Query: 396 RAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQLR 455
            A+ +++  Y AWYGLG  Y       ++ H+F+ +  + P+ S +   +     +  L+
Sbjct: 581 NALRVDTRHYNAWYGLGMIYLRQEKLEFSEHHFRMAFLINPSSSVIMSYLGTSLHA--LK 640

Query: 456 MLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNM 515
             EEA++   +A   + +  + ++Q A +   L + +EA       LE +E  +   P+ 
Sbjct: 641 RSEEALEIMEQAIVADRKNPLPMYQKANILVCLERLDEA-------LEVLEELKEYAPSE 700

Query: 516 VEALLFLATYYKAQKKFDEAEVYCTRLLDYTGPEKETA 554
                 +   YK +   D+A ++    LD   P  + A
Sbjct: 701 SSVYALMGRIYKRRNMHDKAMLHFGLALDMKPPATDVA 725

BLAST of Cp4.1LG20g02180 vs. TAIR 10
Match: AT3G16320.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.9e-30
Identity = 86/319 (26.96%), Postives = 152/319 (47.65%), Query Frame = 0

Query: 241 LAYAYQELRMH--NESMVKYENLQATFGFSNYIQAQIAKAQYSLREFDQVEAIFEELLRN 300
           L   ++ L M+   E+++ Y+ L      ++++  Q+ KA + L+++   ++ F    + 
Sbjct: 391 LGDGHRHLHMYKCQEALLAYQKLSQKQYNTHWVLMQVGKAYFELQDYFNADSSFTLAHQK 450

Query: 301 DPYRVEDMDMYSNVLYAKECFSALSYLAHRVFMTDKYRPESCCIIGNYYSLKGQHEKSVV 360
            PY +E MD YS VLY  +    L YLA  +   D+  PES C +GN YSL+  H+ ++ 
Sbjct: 451 YPYALEGMDTYSTVLYHLKEEMRLGYLAQELISVDRLSPESWCAVGNCYSLRKDHDTALK 510

Query: 361 YFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDINSCDYRAWYGLGQAYEMM 420
            F+RA++LN+ +  A TL GHEF  ++    A   YR+A+ I++  Y AWYGLG  Y   
Sbjct: 511 MFQRAIQLNERFTYAHTLCGHEFAALEEFEDAERCYRKALGIDTRHYNAWYGLGMTYLRQ 570

Query: 421 GMPFYALHYFKKSVFLQPNDSRLWIAMAQCYES---EQLRMLEEAIKCYRRAASCNDREA 480
               +A H F+ ++ + P  S     +  CY      + +  +EA+    +A   + +  
Sbjct: 571 EKFEFAQHQFQLALQINPRSS-----VIMCYYGIALHESKRNDEALMMMEKAVLTDAKNP 630

Query: 481 IALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGPNMVEALLFLATYYKAQKKFDEA 540
           +  +  A + + LG        Y K  + +E  +   P        L   Y   K++D+A
Sbjct: 631 LPKYYKAHILTSLGD-------YHKAQKVLEELKECAPQESSVHASLGKIYNQLKQYDKA 690

Query: 541 EVYCTRLLDYTGPEKETAK 555
            ++    LD +    +  K
Sbjct: 691 VLHFGIALDLSPSPSDAVK 697

BLAST of Cp4.1LG20g02180 vs. TAIR 10
Match: AT3G04240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-11
Identity = 69/262 (26.34%), Postives = 110/262 (41.98%), Query Frame = 0

Query: 275 IAKAQYSLREFDQVEAIFEELLRNDPYRVEDMDMYSNVLYAK-ECFSALSYLAHRVFMTD 334
           I    Y L+E+D   A  EE LR  P   E     +N    K +   A+ Y    + +  
Sbjct: 93  IGAIYYQLQEYDMCIARNEEALRIQPQFAECYGNMANAWKEKGDTDRAIRYYLIAIELRP 152

Query: 335 KYRPESCCIIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDA 394
            +  ++   + + Y  KG+  ++    ++AL LN   + A + +G+       I  A   
Sbjct: 153 NF-ADAWSNLASAYMRKGRLSEATQCCQQALSLNPLLVDAHSNLGNLMKAQGLIHEAYSC 212

Query: 395 YRRAVDINSCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDSRLWIAMAQCYESEQ 454
           Y  AV I      AW  L   +   G    AL Y+K++V L+P     ++ +   Y++  
Sbjct: 213 YLEAVRIQPTFAIAWSNLAGLFMESGDLNRALQYYKEAVKLKPAFPDAYLNLGNVYKA-- 272

Query: 455 LRMLEEAIKCYRRAASCNDREAIALHQLAKLHSELGQSEEAAFYYKKDLERMEAEEREGP 514
           L    EAI CY+ A       A+A   +A ++ E GQ + A  +YK+ L R        P
Sbjct: 273 LGRPTEAIMCYQHALQMRPNSAMAFGNIASIYYEQGQLDLAIRHYKQALSR-------DP 332

Query: 515 NMVEALLFLATYYKAQKKFDEA 536
             +EA   L    K   + DEA
Sbjct: 333 RFLEAYNNLGNALKDIGRVDEA 344

BLAST of Cp4.1LG20g02180 vs. TAIR 10
Match: AT5G56290.1 (peroxin 5 )

HSP 1 Score: 58.2 bits (139), Expect = 2.6e-08
Identity = 27/101 (26.73%), Postives = 51/101 (50.50%), Query Frame = 0

Query: 342 IIGNYYSLKGQHEKSVVYFRRALKLNKNYLSAWTLMGHEFVEMKNIPAAIDAYRRAVDIN 401
           ++G  Y+L  + ++++  F+ AL+L  N  S W  +G           AI AY++A+D+ 
Sbjct: 595 VLGVLYNLSREFDRAITSFQTALQLKPNDYSLWNKLGATQANSVQSADAISAYQQALDLK 654

Query: 402 SCDYRAWYGLGQAYEMMGMPFYALHYFKKSVFLQPNDSRLW 443
               RAW  +G +Y   GM   ++ Y+ +++ + P     W
Sbjct: 655 PNYVRAWANMGISYANQGMYKESIPYYVRALAMNPKADNAW 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STS37.3e-27479.07Anaphase-promoting complex subunit 8 OS=Arabidopsis thaliana OX=3702 GN=APC8 PE=... [more]
Q86B119.3e-12843.65Anaphase-promoting complex subunit 8 OS=Dictyostelium discoideum OX=44689 GN=ana... [more]
A1A4R83.0e-11847.61Cell division cycle protein 23 homolog OS=Bos taurus OX=9913 GN=CDC23 PE=2 SV=1[more]
Q9UJX23.0e-11847.61Cell division cycle protein 23 homolog OS=Homo sapiens OX=9606 GN=CDC23 PE=1 SV=... [more]
Q8BGZ42.0e-11747.40Cell division cycle protein 23 homolog OS=Mus musculus OX=10090 GN=Cdc23 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_023519242.10.0100.00anaphase-promoting complex subunit 8 [Cucurbita pepo subsp. pepo][more]
KAG6584242.10.099.31Anaphase-promoting complex subunit 8, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022924091.10.098.96anaphase-promoting complex subunit 8 [Cucurbita moschata][more]
XP_023000742.10.098.79anaphase-promoting complex subunit 8 [Cucurbita maxima][more]
XP_004152456.10.096.53anaphase-promoting complex subunit 8 [Cucumis sativus] >KGN64281.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
A0A6J1E8K90.098.96anaphase-promoting complex subunit 8 OS=Cucurbita moschata OX=3662 GN=LOC1114316... [more]
A0A6J1KEI30.098.79anaphase-promoting complex subunit 8 OS=Cucurbita maxima OX=3661 GN=LOC111495101... [more]
A0A0A0LW670.096.53TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045790 ... [more]
A0A1S3AVU00.096.71anaphase-promoting complex subunit 8 OS=Cucumis melo OX=3656 GN=LOC103483216 PE=... [more]
A0A6J1K5K00.096.36anaphase-promoting complex subunit 8-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT3G48150.15.2e-27579.07anaphase-promoting complex subunit 8 [more]
AT2G20000.19.9e-3227.22CDC27 family protein [more]
AT3G16320.11.9e-3026.96Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G04240.11.1e-1126.34Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G56290.12.6e-0826.73peroxin 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..171
NoneNo IPR availablePFAMPF13414TPR_11coord: 378..419
e-value: 7.2E-8
score: 32.0
NoneNo IPR availablePANTHERPTHR12558CELL DIVISION CYCLE 16,23,27coord: 4..576
NoneNo IPR availablePANTHERPTHR12558:SF37BNAA06G16860D PROTEINcoord: 4..576
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 371..404
e-value: 1.5
score: 17.8
coord: 269..302
e-value: 230.0
score: 3.0
coord: 337..370
e-value: 7.6E-5
score: 32.1
coord: 475..508
e-value: 11.0
score: 14.5
coord: 439..474
e-value: 26.0
score: 11.4
coord: 405..438
e-value: 5.6E-4
score: 29.2
coord: 516..549
e-value: 2.8
score: 16.9
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 338..369
e-value: 3.5E-5
score: 23.7
IPR019734Tetratricopeptide repeatPFAMPF13176TPR_7coord: 477..506
e-value: 0.0033
score: 17.4
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 371..404
score: 9.4699
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 405..438
score: 9.8829
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 337..370
score: 8.8504
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 334..544
e-value: 1.5E-56
score: 193.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 2..333
e-value: 5.1E-103
score: 346.2
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 337..503
IPR007192Cdc23PFAMPF04049ANAPC8coord: 7..157
e-value: 2.1E-41
score: 141.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g02180.1Cp4.1LG20g02180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0051301 cell division
biological_process GO:0045842 positive regulation of mitotic metaphase/anaphase transition
biological_process GO:0016567 protein ubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
cellular_component GO:0005680 anaphase-promoting complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding